Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,347,664 A→G T182T (ACT→ACC)  yciW ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,347,6640AG99.6% 846.7 / ‑4.9 262T182T (ACT→ACC) yciWputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (116/144);  total (118/144)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

Only 100 of 267 total aligned reads displayed.

ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG  >  NC_000913/1347574‑1347753
                                                                                          |                                                                                         
 tGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTa                                                                                        <  1:9763926/93‑1 (MQ=255)
 tGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTa                                                                                        >  2:7296468/1‑93 (MQ=255)
   cccGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGCTCACACAGTCGCAGGCGGTAAg                                                                                      >  1:3996609/1‑93 (MQ=255)
     cGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAc                                                                                    <  2:2775345/93‑1 (MQ=255)
      gagaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAcc                                                                                   >  2:3771175/1‑93 (MQ=255)
       agaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCg                                                                                  >  1:2249656/1‑93 (MQ=255)
       agaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCg                                                                                  <  1:2723528/93‑1 (MQ=255)
         aTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAc                                                                                >  1:1627902/1‑93 (MQ=255)
         aTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAc                                                                                <  2:8849318/93‑1 (MQ=255)
         aTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAc                                                                                >  1:6605233/1‑93 (MQ=255)
          tAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACg                                                                               >  1:6733916/1‑93 (MQ=255)
          tAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACg                                                                               >  2:6698641/1‑93 (MQ=255)
            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             >  1:7801894/1‑93 (MQ=255)
            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             <  1:3334420/93‑1 (MQ=255)
             gCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCa                                                                            <  2:9060037/93‑1 (MQ=255)
              ccTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCAt                                                                           >  1:158026/1‑93 (MQ=255)
               cTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTGCCAGGCGGTAAGACCGACGTCAtt                                                                          >  2:982283/1‑93 (MQ=255)
                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         >  2:620286/1‑93 (MQ=255)
                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         <  1:7244828/93‑1 (MQ=255)
                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         >  1:7311368/1‑93 (MQ=255)
                 gAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCa                                                                        <  1:8040998/93‑1 (MQ=255)
                   aaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGa                                                                      <  2:1641721/93‑1 (MQ=255)
                   aaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGa                                                                      >  2:6578972/1‑93 (MQ=255)
                    aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     <  2:26144/93‑1 (MQ=255)
                    aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     >  2:6449886/1‑93 (MQ=255)
                      gTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGc                                                                   <  1:2500776/93‑1 (MQ=255)
                         gCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTgg                                                                >  1:5877878/1‑93 (MQ=255)
                         gCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTgg                                                                <  2:7221903/93‑1 (MQ=255)
                          cAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgc                                                               <  1:9238021/93‑1 (MQ=255)
                            aTTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgcgc                                                             <  1:6968166/93‑1 (MQ=255)
                             ttGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGcgcg                                                            >  2:7129819/1‑93 (MQ=255)
                               gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGAt                                                          <  2:590819/93‑1 (MQ=255)
                               gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGAt                                                          <  1:7405603/93‑1 (MQ=255)
                               gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGAt                                                          <  1:1057688/93‑1 (MQ=255)
                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  1:6013658/1‑93 (MQ=255)
                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         <  2:8094588/93‑1 (MQ=255)
                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  2:2017695/1‑93 (MQ=255)
                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  2:7475814/1‑93 (MQ=255)
                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCAAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  2:3002227/1‑93 (MQ=255)
                                  cgcgCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGTGCGATCgg                                                       <  1:5596675/93‑1 (MQ=255)
                                  cgcgCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCgg                                                       <  1:3965480/93‑1 (MQ=255)
                                   gcgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGt                                                      <  1:9593532/93‑1 (MQ=255)
                                   gcgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGt                                                      <  1:5751258/93‑1 (MQ=255)
                                     gcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTg                                                    >  1:9480932/1‑93 (MQ=255)
                                      ccTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGt                                                   <  1:4502404/93‑1 (MQ=255)
                                         gAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTTTCGa                                                >  2:3546648/1‑93 (MQ=255)
                                         gAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGa                                                <  1:1007625/93‑1 (MQ=255)
                                          aaaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGat                                               >  1:9866323/1‑93 (MQ=255)
                                           aaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGata                                              <  1:3511452/93‑1 (MQ=255)
                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             >  2:434486/1‑93 (MQ=255)
                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             <  1:5023370/93‑1 (MQ=255)
                                              ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataata                                           <  1:4810998/93‑1 (MQ=255)
                                               cAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAg                                          <  1:1900075/93‑1 (MQ=255)
                                               cAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAg                                          <  1:9867008/93‑1 (MQ=255)
                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        <  1:1757706/93‑1 (MQ=255)
                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        >  2:1351391/1‑93 (MQ=255)
                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        <  2:8419113/93‑1 (MQ=255)
                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       <  2:4398579/93‑1 (MQ=255)
                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       <  1:6569071/93‑1 (MQ=255)
                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAACGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       >  2:4935629/1‑93 (MQ=255)
                                                     aaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCg                                    <  2:7367604/93‑1 (MQ=255)
                                                       ccAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAg                                  >  1:9190617/1‑93 (MQ=255)
                                                       ccAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAg                                  <  2:2500083/93‑1 (MQ=255)
                                                        cAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGc                                 >  1:7972503/1‑93 (MQ=255)
                                                         aaTGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCt                                >  2:8434688/1‑93 (MQ=255)
                                                            gATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGc                             >  1:5474530/1‑93 (MQ=255)
                                                             aTTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGcc                            <  2:181823/93‑1 (MQ=255)
                                                             aTTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGcc                            <  1:8860431/93‑1 (MQ=255)
                                                              tttGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCa                           <  1:6343439/93‑1 (MQ=255)
                                                                 ggCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGtt                        <  2:8865822/93‑1 (MQ=255)
                                                                 ggCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGtt                        <  1:5436472/93‑1 (MQ=255)
                                                                  gCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTg                       >  1:8746903/1‑93 (MQ=255)
                                                                  gCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTg                       <  2:5133368/93‑1 (MQ=255)
                                                                   cTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTgg                      <  1:1389618/93‑1 (MQ=255)
                                                                     aaTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCg                    >  2:9458472/1‑93 (MQ=255)
                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:3319324/1‑93 (MQ=255)
                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:3612126/1‑93 (MQ=255)
                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:367013/1‑93 (MQ=255)
                                                                       tGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCggg                  >  1:6864472/1‑93 (MQ=255)
                                                                       tGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCggg                  >  2:2303122/1‑93 (MQ=255)
                                                                        gATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGc                 >  2:1523794/1‑93 (MQ=255)
                                                                         aTCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCa                >  2:3035712/1‑93 (MQ=255)
                                                                           cacacaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCata              >  2:4076332/1‑93 (MQ=255)
                                                                             cacaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGTGGGCATAtt            <  2:6737520/93‑1 (MQ=255)
                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           <  1:6174347/93‑1 (MQ=255)
                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           <  1:8295292/93‑1 (MQ=255)
                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           >  2:1726221/1‑93 (MQ=255)
                                                                               caGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGc          >  2:855447/1‑93 (MQ=255)
                                                                                aGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCg         <  2:7579671/93‑1 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGGCCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  2:3353322/93‑1 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  2:5046639/93‑1 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:1155858/1‑93 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:9428449/1‑93 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:4638430/1‑93 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:297609/1‑93 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  1:3202615/93‑1 (MQ=255)
                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  2:5004224/1‑93 (MQ=255)
                                                                                    ccAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAt     >  1:7509519/1‑93 (MQ=255)
                                                                                     cAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAtt    >  2:6018529/1‑93 (MQ=255)
                                                                                      aGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAttt   <  1:6898886/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG  >  NC_000913/1347574‑1347753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCC  >  NC_000913/1347571‑1347757
                                                                                             |                                                                                             
CGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCG                                                                                               <  SRR4302540.2760618/94‑1 (MQ=60)
CGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCG                                                                                               >  SRR4302540.1566161/1‑94 (MQ=60)
 GGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGG                                                                                              >  SRR4302540.4718081/1‑94 (MQ=60)
  GATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGT                                                                                             <  SRR4302540.5157095/94‑1 (MQ=60)
   ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTA                                                                                            <  SRR4302540.6230684/94‑1 (MQ=60)
    TGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAA                                                                                           <  SRR4302540.9878433/94‑1 (MQ=60)
     GCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGCTCACACAGTCGCAGGCGGTAAG                                                                                          >  SRR4302540.4039619/1‑94 (MQ=60)
     GCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAG                                                                                          <  SRR4302540.6671302/94‑1 (MQ=60)
     GCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAG                                                                                          <  SRR4302540.991057/94‑1 (MQ=60)
     GCCCGAGATAAGCCTGAAATGTCGCAATTGTCCCCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAA                                                                                          >  SRR4302540.4039618/1‑94 (MQ=60)
       CCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAC                                                                                        >  SRR4302540.8730564/1‑94 (MQ=60)
        CGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACC                                                                                       <  SRR4302540.2404874/94‑1 (MQ=60)
         GAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCG                                                                                      <  SRR4302540.5916825/94‑1 (MQ=60)
         GAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCG                                                                                      >  SRR4302540.2273810/1‑94 (MQ=60)
         GAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCG                                                                                      >  SRR4302540.6459903/1‑94 (MQ=60)
          AGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGA                                                                                     <  SRR4302540.2752894/94‑1 (MQ=60)
           GATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACCCAGTCCCAGGCGGGAAGACCGAC                                                                                    <  SRR4302540.6922210/94‑1 (MQ=60)
           GATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAC                                                                                    >  SRR4302540.1645729/1‑94 (MQ=60)
           GATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAC                                                                                    >  SRR4302540.6678375/1‑94 (MQ=60)
           GATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAC                                                                                    >  SRR4302540.3189931/1‑94 (MQ=60)
            ATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACG                                                                                   >  SRR4302540.5616085/1‑94 (MQ=60)
            ATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACG                                                                                   >  SRR4302540.6808285/1‑94 (MQ=60)
             TAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGT                                                                                  >  SRR4302540.9498112/1‑94 (MQ=60)
             TAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGT                                                                                  >  SRR4302540.1294753/1‑94 (MQ=60)
              AAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTC                                                                                 >  SRR4302540.7893078/1‑94 (MQ=60)
               AGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCA                                                                                <  SRR4302540.3371036/94‑1 (MQ=60)
                GCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCAT                                                                               <  SRR4302540.6642093/94‑1 (MQ=60)
                GCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCAT                                                                               <  SRR4302540.7392779/94‑1 (MQ=60)
                GCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCAT                                                                               >  SRR4302540.159624/1‑94 (MQ=60)
                  CTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTC                                                                             >  SRR4302540.7393000/1‑94 (MQ=60)
                  CTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAAAGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTC                                                                             >  SRR4302540.8209595/1‑94 (MQ=60)
                   TGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCA                                                                            >  SRR4302540.7371973/1‑94 (MQ=60)
                   TGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCA                                                                            <  SRR4302540.7325655/94‑1 (MQ=60)
                    GAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAG                                                                           <  SRR4302540.8134430/94‑1 (MQ=60)
                    GAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAG                                                                           <  SRR4302540.7223377/94‑1 (MQ=60)
                    GAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAG                                                                           <  SRR4302540.6754828/94‑1 (MQ=60)
                     AAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGA                                                                          >  SRR4302540.3822958/1‑94 (MQ=60)
                     AAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGA                                                                          <  SRR4302540.1647844/94‑1 (MQ=60)
                      AATGTCGCAATTGTCCGCGCCTGAAATCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAT                                                                         <  SRR4302540.6838506/94‑1 (MQ=60)
                      AATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAT                                                                         <  SRR4302540.2953432/94‑1 (MQ=60)
                        AGTCGCAATTGTCCGCGCCTGAAAGCCAATAAACCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGACATTCAGATGC                                                                       <  SRR4302540.9034340/94‑1 (MQ=60)
                         GTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCT                                                                      >  SRR4302540.6944430/1‑94 (MQ=60)
                         GTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCT                                                                      <  SRR4302540.2527759/94‑1 (MQ=60)
                         GTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCT                                                                      >  SRR4302540.439657/1‑94 (MQ=60)
                          TCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTG                                                                     >  SRR4302540.3459141/1‑94 (MQ=60)
                           CGGAAGTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACTCAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGG                                                                    <  SRR4302540.4233658/94‑1 (MQ=60)
                           CGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGG                                                                    >  SRR4302540.5942846/1‑94 (MQ=60)
                           CGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGG                                                                    <  SRR4302540.4233659/94‑1 (MQ=60)
                           CGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACAGACGTCATTCAGATGCTGG                                                                    >  SRR4302540.7514210/1‑94 (MQ=60)
                            GCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGC                                                                   <  SRR4302540.9179022/94‑1 (MQ=60)
                            GCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGC                                                                   >  SRR4302540.2027491/1‑94 (MQ=60)
                             CAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCG                                                                  <  SRR4302540.9348690/94‑1 (MQ=60)
                              AATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGC                                                                 >  SRR4302540.993005/1‑94 (MQ=60)
                               ATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCG                                                                <  SRR4302540.7045633/94‑1 (MQ=60)
                                  GTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATC                                                             <  SRR4302540.1068455/94‑1 (MQ=60)
                                  GTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATC                                                             >  SRR4302540.6080346/1‑94 (MQ=60)
                                  GTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATC                                                             <  SRR4302540.7488105/94‑1 (MQ=60)
                                  GTCCGCGCCTGAAAGCCAATAAAACCAATGATTCGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATC                                                             <  SRR4302540.3579515/94‑1 (MQ=60)
                                   TCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCG                                                            <  SRR4302540.1505057/94‑1 (MQ=60)
                                   TCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCG                                                            <  SRR4302540.3687182/94‑1 (MQ=60)
                                   TCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCG                                                            <  SRR4302540.7850230/94‑1 (MQ=60)
                                    CCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGG                                                           <  SRR4302540.3773094/94‑1 (MQ=60)
                                     CGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGTGCGATCGGT                                                          <  SRR4302540.5658249/94‑1 (MQ=60)
                                     CGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGT                                                          <  SRR4302540.4008232/94‑1 (MQ=60)
                                      GCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTT                                                         <  SRR4302540.5814735/94‑1 (MQ=60)
                                      GCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTT                                                         <  SRR4302540.2483706/94‑1 (MQ=60)
                                      GCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTT                                                         <  SRR4302540.9706744/94‑1 (MQ=60)
                                       CGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTG                                                        >  SRR4302540.9593359/1‑94 (MQ=60)
                                         CCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTC                                                      <  SRR4302540.4551210/94‑1 (MQ=60)
                                          CTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCG                                                     >  SRR4302540.7401275/1‑94 (MQ=60)
                                          CTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCG                                                     <  SRR4302540.1922356/94‑1 (MQ=60)
                                          CTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCG                                                     >  SRR4302540.954795/1‑94 (MQ=60)
                                          CTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTAG                                                     <  SRR4302540.9348799/94‑1 (MQ=60)
                                           TGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGA                                                    <  SRR4302540.8122585/94‑1 (MQ=60)
                                            GAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGAT                                                   <  SRR4302540.5711556/94‑1 (MQ=60)
                                            GAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGAT                                                   >  SRR4302540.9981574/1‑94 (MQ=60)
                                            GAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGAT                                                   <  SRR4302540.1017846/94‑1 (MQ=60)
                                            GAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGAA                                                   >  SRR4302540.7773414/1‑94 (MQ=60)
                                             AAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATA                                                  >  SRR4302540.1059827/1‑94 (MQ=60)
                                              AAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAA                                                 <  SRR4302540.3549705/94‑1 (MQ=60)
                                               AGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAAT                                                <  SRR4302540.5077746/94‑1 (MQ=60)
                                                GCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATA                                               <  SRR4302540.9550506/94‑1 (MQ=60)
                                                 CCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAG                                              <  SRR4302540.4862493/94‑1 (MQ=60)
                                                 CCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAG                                              <  SRR4302540.1636689/94‑1 (MQ=60)
                                                  CAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGA                                             >  SRR4302540.9333273/1‑94 (MQ=60)
                                                  CAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGA                                             <  SRR4302540.9982262/94‑1 (MQ=60)
                                                  CAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGA                                             <  SRR4302540.1920857/94‑1 (MQ=60)
                                                  CAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGA                                             >  SRR4302540.3154312/1‑94 (MQ=60)
                                                  CAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGA                                             <  SRR4302540.9982261/94‑1 (MQ=60)
                                                   AATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAA                                            <  SRR4302540.6053014/94‑1 (MQ=60)
                                                    ATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAG                                           <  SRR4302540.164732/94‑1 (MQ=60)
                                                    ATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAG                                           <  SRR4302540.1776756/94‑1 (MQ=60)
                                                    ATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAG                                           <  SRR4302540.7505810/94‑1 (MQ=60)
                                                    ATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAG                                           <  SRR4302540.5520607/94‑1 (MQ=60)
                                                    ATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAG                                           <  SRR4302540.5033139/94‑1 (MQ=60)
                                                    ATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAG                                           <  SRR4302540.4633331/94‑1 (MQ=60)
                                                     TAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGG                                          <  SRR4302540.6641861/94‑1 (MQ=60)
                                                     AAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGG                                          <  SRR4302540.3526240/94‑1 (MQ=60)
                                                       AAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCG                                        <  SRR4302540.1173861/94‑1 (MQ=60)
                                                       AAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCG                                        <  SRR4302540.3368261/94‑1 (MQ=60)
                                                        AACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGA                                       <  SRR4302540.4791018/94‑1 (MQ=60)
                                                         ACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAG                                      >  SRR4302540.9300845/1‑94 (MQ=60)
                                                         ACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAG                                      >  SRR4302540.629426/1‑94 (MQ=60)
                                                         ACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAG                                      <  SRR4302540.6999207/94‑1 (MQ=60)
                                                          CCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGC                                     >  SRR4302540.8065395/1‑94 (MQ=60)
                                                          CCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGC                                     <  SRR4302540.7973324/94‑1 (MQ=60)
                                                             ATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAG                                  <  SRR4302540.5764181/94‑1 (MQ=60)
                                                              TGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGC                                 >  SRR4302540.5534789/1‑94 (MQ=60)
                                                                ATTTGGTTAATGATCACACAGTCCTAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCA                               <  SRR4302540.8962955/94‑1 (MQ=60)
                                                                ATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCA                               <  SRR4302540.8962956/94‑1 (MQ=60)
                                                                 TTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAG                              <  SRR4302540.6414078/94‑1 (MQ=60)
                                                                  TTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGT                             >  SRR4302540.9830662/1‑94 (MQ=60)
                                                                  TTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGT                             <  SRR4302540.522602/94‑1 (MQ=60)
                                                                   TGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTT                            <  SRR4302540.5366607/94‑1 (MQ=60)
                                                                    GGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTG                           >  SRR4302540.8848406/1‑94 (MQ=60)
                                                                    GGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTG                           <  SRR4302540.3358631/94‑1 (MQ=60)
                                                                    GGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTG                           <  SRR4302540.5496270/94‑1 (MQ=60)
                                                                     GCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGG                          <  SRR4302540.8091811/94‑1 (MQ=60)
                                                                      CTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGC                         <  SRR4302540.1404487/94‑1 (MQ=60)
                                                                      CTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGC                         >  SRR4302540.4930800/1‑94 (MQ=60)
                                                                       TAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCG                        <  SRR4302540.7184436/94‑1 (MQ=60)
                                                                        AATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGG                       >  SRR4302540.2732880/1‑94 (MQ=60)
                                                                        AATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGG                       >  SRR4302540.3355736/1‑94 (MQ=60)
                                                                        AATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGG                       >  SRR4302540.370793/1‑94 (MQ=60)
                                                                        AATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGG                       >  SRR4302540.3651615/1‑94 (MQ=60)
                                                                         ATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGG                      >  SRR4302540.6940841/1‑94 (MQ=60)
                                                                         ATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGG                      >  SRR4302540.6940546/1‑94 (MQ=60)
                                                                         ATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGG                      <  SRR4302540.9627702/94‑1 (MQ=60)
                                                                         ATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGG                      <  SRR4302540.1834683/94‑1 (MQ=60)
                                                                           GATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCA                    <  SRR4302540.6045916/94‑1 (MQ=60)
                                                                            ATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCAT                   <  SRR4302540.9831315/94‑1 (MQ=60)
                                                                            ATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCAT                   >  SRR4302540.8899480/1‑94 (MQ=60)
                                                                            ATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATACTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCAT                   >  SRR4302540.2913535/1‑94 (MQ=60)
                                                                             TCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATA                  >  SRR4302540.4626852/1‑94 (MQ=60)
                                                                               ACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATT                <  SRR4302540.1071128/94‑1 (MQ=60)
                                                                               ACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATT                <  SRR4302540.1071126/94‑1 (MQ=60)
                                                                                 ACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGC              >  SRR4302540.3707278/1‑94 (MQ=60)
                                                                                 ACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGC              <  SRR4302540.6243140/94‑1 (MQ=60)
                                                                                 ACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGC              <  SRR4302540.8391615/94‑1 (MQ=60)
                                                                                 ACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGC              >  SRR4302540.6415420/1‑94 (MQ=60)
                                                                                   AGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGT            >  SRR4302540.300557/1‑94 (MQ=60)
                                                                                   AGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGT            >  SRR4302540.1167888/1‑94 (MQ=60)
                                                                                   AGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGT            >  SRR4302540.9540465/1‑94 (MQ=60)
                                                                                   AGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGT            >  SRR4302540.4688093/1‑94 (MQ=60)
                                                                                    GTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTG           >  SRR4302540.5541256/1‑94 (MQ=60)
                                                                                    GTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTG           <  SRR4302540.3237398/94‑1 (MQ=60)
                                                                                      CCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAT         >  SRR4302540.7593456/1‑94 (MQ=60)
                                                                                         AGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG      <  SRR4302540.1644416/94‑1 (MQ=60)
                                                                                         AGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG      >  SRR4302540.8314070/1‑94 (MQ=60)
                                                                                         AGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG      <  SRR4302540.6975505/94‑1 (MQ=60)
                                                                                          GGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGT     <  SRR4302540.3237546/94‑1 (MQ=60)
                                                                                          GGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCACATTGCGTGATTTGT     <  SRR4302540.6239264/94‑1 (MQ=60)
                                                                                           GCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTG    <  SRR4302540.742689/94‑1 (MQ=60)
                                                                                            CGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGC   <  SRR4302540.2305959/94‑1 (MQ=60)
                                                                                             GGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCC  <  SRR4302540.148329/94‑1 (MQ=60)
                                                                                             GGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCC  <  SRR4302540.1376395/94‑1 (MQ=60)
                                                                                             GGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCC  >  SRR4302540.7349390/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCC  >  NC_000913/1347571‑1347757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑