Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,962	C→T	R194H (CGT→CAT)	sapC ←	SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,962	0	C	T	99.6%	847.5 / NA	230	R194H (CGT→CAT)	sapC	SapC
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (113/116); minor base G (0/1); total (113/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

AGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  NC_000913/1351872‑1352051

 gTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATggg                                                                                        >  1:1619524/1‑93 (MQ=255)

  tGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGggc                                                                                       <  1:2262492/93‑1 (MQ=255)

      cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGgcggcg                                                                                   <  1:4425906/93‑1 (MQ=255)

       gggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGa                                                                                  >  1:1443782/1‑93 (MQ=255)

       gggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGa                                                                                  <  1:6361130/93‑1 (MQ=255)

         gTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATa                                                                                <  2:5589106/93‑1 (MQ=255)

         gTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATa                                                                                <  1:9925154/93‑1 (MQ=255)

         gTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATa                                                                                <  1:8827928/93‑1 (MQ=255)

          tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATaa                                                                               <  1:5610158/93‑1 (MQ=255)

            aTCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACg                                                                             <  2:8222490/93‑1 (MQ=255)

             tctcGGTGACCAGCCCGGCGGTGATGTTTGGCCTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGt                                                                            <  2:7951607/93‑1 (MQ=255)

             tctcGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGt                                                                            >  1:10016027/1‑93 (MQ=255)

             tctcGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGt                                                                            <  1:2935692/93‑1 (MQ=255)

               tcGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTAc                                                                          >  2:4772639/1‑93 (MQ=255)

                cGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTAct                                                                         <  2:5227422/93‑1 (MQ=255)

                 ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActc                                                                        <  2:9692624/93‑1 (MQ=255)

                 ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActc                                                                        >  1:3784684/1‑93 (MQ=255)

                  gTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActct                                                                       <  2:6291266/93‑1 (MQ=255)

                   tGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCtt                                                                      >  2:7534767/1‑93 (MQ=255)

                     aCCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCtttt                                                                    <  2:8785340/93‑1 (MQ=255)

                       cAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTc                                                                  >  1:3841670/1‑93 (MQ=255)

                         gCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                                >  2:5684911/1‑93 (MQ=255)

                         gCCCGGCGGTGATGTATGGCATCCCAGCAAACCTGAGAATATTCAACGTTGATGCGCCATACAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                                <  1:8979566/93‑1 (MQ=255)

                            cggcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGtt                                                             >  1:8520052/1‑93 (MQ=255)

                             ggcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTc                                                            <  1:9810447/93‑1 (MQ=255)

                              gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGTCGGCGATAACGTACTTTTTTTCCAGTTCg                                                           >  1:8412254/1‑93 (MQ=255)

                              gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCg                                                           >  1:9445651/1‑93 (MQ=255)

                              gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCg                                                           <  2:4900572/93‑1 (MQ=255)

                               cggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCAAGTTCGt                                                          >  2:1914995/1‑93 (MQ=255)

                                ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTTGTc                                                         >  2:5692712/1‑93 (MQ=255)

                                ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTc                                                         >  1:2491621/1‑93 (MQ=255)

                                ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTc                                                         <  2:9082538/93‑1 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        >  1:2661526/1‑93 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        <  2:5853060/93‑1 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        >  2:8996545/1‑93 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        <  2:964901/93‑1 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       >  2:2864614/1‑93 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       >  2:8202229/1‑93 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       <  1:8224936/93‑1 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       <  1:7108942/93‑1 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       <  1:5430579/93‑1 (MQ=255)

                                    aTGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGc                                                     >  1:4071086/1‑93 (MQ=255)

                                     tGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCa                                                    >  1:4851265/1‑93 (MQ=255)

                                     tGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCa                                                    >  2:3098494/1‑93 (MQ=255)

                                     tGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCa                                                    >  2:2219753/1‑93 (MQ=255)

                                      gTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAc                                                   >  1:6400388/1‑93 (MQ=255)

                                      gTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAc                                                   <  2:8008001/93‑1 (MQ=255)

                                       tttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAcc                                                  >  1:2810476/1‑93 (MQ=255)

                                       tttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAcc                                                  >  1:5099204/1‑93 (MQ=255)

                                       tttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAcc                                                  >  1:7234920/1‑93 (MQ=255)

                                         tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAt                                                >  1:9174929/1‑93 (MQ=255)

                                         tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAt                                                <  1:1089383/93‑1 (MQ=255)

                                          tgCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATg                                               <  1:1998043/92‑1 (MQ=255)

                                           gCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGTCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGc                                              >  2:4168026/1‑93 (MQ=255)

                                            cATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCt                                             <  2:3657205/93‑1 (MQ=255)

                                            cATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCt                                             >  2:3821065/1‑93 (MQ=255)

                                             aTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTg                                            >  1:7893799/1‑93 (MQ=255)

                                             aTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTg                                            <  1:1266803/93‑1 (MQ=255)

                                              tCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGt                                           >  2:4053701/1‑93 (MQ=255)

                                                  aGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAAt                                       <  2:6665821/93‑1 (MQ=255)

                                                   gCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATc                                      <  2:2923082/93‑1 (MQ=255)

                                                    cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                     >  2:328229/1‑93 (MQ=255)

                                                    cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                     <  2:6745638/93‑1 (MQ=255)

                                                    cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                     <  2:7624036/93‑1 (MQ=255)

                                                     aaaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGa                                    >  1:9064065/1‑93 (MQ=255)

                                                      aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                   >  2:5564/1‑93 (MQ=255)

                                                      aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                   <  1:1037377/93‑1 (MQ=255)

                                                      aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                   >  2:2494466/1‑93 (MQ=255)

                                                       aCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAc                                  >  1:6292799/1‑93 (MQ=255)

                                                          agagAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTa                               <  2:2770634/93‑1 (MQ=255)

                                                           gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                              <  2:7895397/93‑1 (MQ=255)

                                                           gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                              >  2:1030051/1‑93 (MQ=255)

                                                           gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                              <  1:670915/93‑1 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            >  1:1407048/1‑93 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            <  2:5452048/93‑1 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            >  2:7432048/1‑93 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            <  1:2176189/93‑1 (MQ=255)

                                                               atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                          <  1:3486019/93‑1 (MQ=255)

                                                               atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                          <  2:8782758/93‑1 (MQ=255)

                                                               atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                          <  2:7229359/93‑1 (MQ=255)

                                                                tatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAc                         <  1:7647458/93‑1 (MQ=255)

                                                                   tCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAcgcg                      >  2:8577543/1‑93 (MQ=255)

                                                                      gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgca                   <  1:5016001/93‑1 (MQ=255)

                                                                      gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgca                   >  2:3327235/1‑93 (MQ=255)

                                                                       cGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGCTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgcag                  >  1:7599971/1‑93 (MQ=255)

                                                                        gTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgcagc                 <  1:841230/93‑1 (MQ=255)

                                                                          ttaTGCCCCAACCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCaa               <  2:8654738/91‑1 (MQ=255)

                                                                          tGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCaa               <  1:8176428/93‑1 (MQ=255)

                                                                          tGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCaa               <  1:597009/93‑1 (MQ=255)

                                                                             tGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgc            >  2:1438461/1‑93 (MQ=255)

                                                                                  cATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcc       >  1:4650901/1‑93 (MQ=255)

                                                                                   aTCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcca      >  2:2367062/1‑93 (MQ=255)

                                                                                   aTCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcca      >  1:1237843/1‑93 (MQ=255)

                                                                                    tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag     >  2:6519107/1‑93 (MQ=255)

                                                                                    tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag     >  1:5825126/1‑93 (MQ=255)

                                                                                    tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag     >  2:395881/1‑93 (MQ=255)

                                                                                      cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc   >  1:1175842/1‑93 (MQ=255)

                                                                                      cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc   <  1:5185149/93‑1 (MQ=255)

                                                                                      cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc   <  2:4981857/93‑1 (MQ=255)

                                                                                       aGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACa  >  2:4061155/1‑93 (MQ=255)

AGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  NC_000913/1351872‑1352051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  NC_000913/1351869‑1352051

GACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGAT                                                                                           >  SRR4302540.5397195/1‑94 (MQ=60)

GACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGAT                                                                                           >  SRR4302540.7693662/1‑94 (MQ=60)

 ACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATG                                                                                          >  SRR4302540.5186437/1‑94 (MQ=60)

   AGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGTCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGG                                                                                        <  SRR4302540.125759/94‑1 (MQ=60)

   AGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGG                                                                                        >  SRR4302540.1637285/1‑94 (MQ=60)

     TGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCG                                                                                      <  SRR4302540.2286772/94‑1 (MQ=60)

     TGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCG                                                                                      >  SRR4302540.2123879/1‑94 (MQ=60)

      GCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGG                                                                                     <  SRR4302540.6392140/94‑1 (MQ=60)

         CGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGA                                                                                  >  SRR4302540.1459475/1‑94 (MQ=60)

         CGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGA                                                                                  <  SRR4302540.4473858/94‑1 (MQ=60)

          GGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAT                                                                                 <  SRR4302540.3832701/94‑1 (MQ=60)

          GGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAT                                                                                 >  SRR4302540.5824300/1‑94 (MQ=60)

          GGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAT                                                                                 <  SRR4302540.6431970/94‑1 (MQ=60)

          GGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGAT                                                                                 <  SRR4302540.6747883/94‑1 (MQ=60)

            GTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAA                                                                               <  SRR4302540.8930018/94‑1 (MQ=60)

            GTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAA                                                                               <  SRR4302540.10041034/94‑1 (MQ=60)

            GTGATCTCGGTGACCAGCCCGGCGGTGATGGTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAA                                                                               <  SRR4302540.4361973/94‑1 (MQ=60)

             TGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAAC                                                                              <  SRR4302540.5671875/94‑1 (MQ=60)

              GATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACG                                                                             <  SRR4302540.1235461/94‑1 (MQ=60)

               ATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGT                                                                            <  SRR4302540.2524423/94‑1 (MQ=60)

               ATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGT                                                                            >  SRR4302540.10132618/1‑94 (MQ=60)

                TCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTA                                                                           <  SRR4302540.2967913/94‑1 (MQ=60)

                 CTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTAC                                                                          <  SRR4302540.1364859/94‑1 (MQ=60)

                  TCGGTGGCCAGCCCGGCGGTGATGTTGGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGGACT                                                                         <  SRR4302540.4939338/94‑1 (MQ=60)

                   CGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTC                                                                        >  SRR4302540.3825957/1‑94 (MQ=60)

                    GGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCT                                                                       <  SRR4302540.7743776/94‑1 (MQ=60)

                    GGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCT                                                                       >  SRR4302540.8611794/1‑94 (MQ=60)

                       GACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTT                                                                    >  SRR4302540.4797464/1‑94 (MQ=60)

                       GACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTT                                                                    <  SRR4302540.9843084/94‑1 (MQ=60)

                         CCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTC                                                                  >  SRR4302540.3883449/1‑94 (MQ=60)

                         CCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTC                                                                  <  SRR4302540.2734924/94‑1 (MQ=60)

                           AGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCA                                                                >  SRR4302540.4018908/1‑94 (MQ=60)

                            GCCCGGCGGTGATGTATGGCATCCCAGCAAACCTGAGAATATTCAACGTTGATGCGCCATACAGATGGGCGGCGATAACGTACTCTTTTTCCAG                                                               <  SRR4302540.9086545/94‑1 (MQ=60)

                              CCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTT                                                             >  SRR4302540.8619070/1‑94 (MQ=60)

                              CCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTT                                                             <  SRR4302540.9974546/94‑1 (MQ=60)

                              CCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTT                                                             >  SRR4302540.2978722/1‑94 (MQ=60)

                                GGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGTCGGCGATAACGTACTTTTTTTCCAGTTCG                                                           >  SRR4302540.8509894/1‑94 (MQ=60)

                                GGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCG                                                           <  SRR4302540.9925262/94‑1 (MQ=60)

                                GGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCG                                                           >  SRR4302540.9557786/1‑94 (MQ=60)

                                GGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCG                                                           >  SRR4302540.8509899/1‑94 (MQ=60)

                                  CGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTC                                                         >  SRR4302540.4871917/1‑94 (MQ=60)

                                  CGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTC                                                         >  SRR4302540.2518456/1‑94 (MQ=60)

                                   GGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCA                                                        >  SRR4302540.2690396/1‑94 (MQ=60)

                                    GTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAT                                                       <  SRR4302540.2517584/94‑1 (MQ=60)

                                    GTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAT                                                       >  SRR4302540.1146596/1‑94 (MQ=60)

                                     TGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATG                                                      <  SRR4302540.8320550/94‑1 (MQ=60)

                                     TGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATG                                                      <  SRR4302540.5490302/94‑1 (MQ=60)

                                     TGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATG                                                      <  SRR4302540.7188059/94‑1 (MQ=60)

                                      GATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGC                                                     >  SRR4302540.4114973/1‑94 (MQ=60)

                                       ATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCA                                                    >  SRR4302540.4903249/1‑94 (MQ=60)

                                       ATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCA                                                    >  SRR4302540.3525823/1‑94 (MQ=60)

                                        TGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAC                                                   >  SRR4302540.6471633/1‑94 (MQ=60)

                                        TGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAC                                                   >  SRR4302540.8478019/1‑94 (MQ=60)

                                        TGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAC                                                   >  SRR4302540.6471632/1‑94 (MQ=60)

                                         GTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACC                                                  >  SRR4302540.2840829/1‑94 (MQ=60)

                                         GTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACC                                                  >  SRR4302540.7315622/1‑94 (MQ=60)

                                         GTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACC                                                  >  SRR4302540.1430023/1‑94 (MQ=60)

                                         GTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACC                                                  >  SRR4302540.6175862/1‑94 (MQ=60)

                                         GTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACC                                                  >  SRR4302540.5154386/1‑94 (MQ=60)

                                           TTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAT                                                >  SRR4302540.9284980/1‑94 (MQ=60)

                                           TTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAT                                                <  SRR4302540.4578608/94‑1 (MQ=60)

                                            TGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATG                                               <  SRR4302540.1100564/94‑1 (MQ=60)

                                             TGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGC                                              <  SRR4302540.2019688/94‑1 (MQ=60)

                                              GCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCT                                             <  SRR4302540.5095295/94‑1 (MQ=60)

                                               CATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTG                                            >  SRR4302540.1699137/1‑94 (MQ=60)

                                               CATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTG                                            >  SRR4302540.7985912/1‑94 (MQ=60)

                                                ATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATCACGTAGTCTTTTTCCAGTTCGTCACGCACCAcgctca                                           >  SRR4302540.5371173/1‑88 (MQ=60)

                                                ATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGT                                           >  SRR4302540.3724536/1‑94 (MQ=60)

                                                ATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGT                                           >  SRR4302540.8764620/1‑94 (MQ=60)

                                                ATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGT                                           <  SRR4302540.1279830/94‑1 (MQ=60)

                                                 TCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTA                                          <  SRR4302540.220097/94‑1 (MQ=60)

                                                 TCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTA                                          <  SRR4302540.220096/94‑1 (MQ=60)

                                                  CACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAA                                         >  SRR4302540.2863560/1‑94 (MQ=60)

                                                  CACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAA                                         <  SRR4302540.10137073/94‑1 (MQ=60)

                                                      GCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCG                                     <  SRR4302540.9454875/94‑1 (MQ=60)

                                                       CAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGA                                    >  SRR4302540.9172897/1‑94 (MQ=60)

                                                        AAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAA                                   >  SRR4302540.1134529/1‑94 (MQ=60)

                                                         AACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTATATCGCAC                                  >  SRR4302540.4930670/1‑94 (MQ=60)

                                                         AACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAC                                  <  SRR4302540.7214437/94‑1 (MQ=60)

                                                         AACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAC                                  >  SRR4302540.6362861/1‑94 (MQ=60)

                                                         AACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAC                                  <  SRR4302540.5742081/94‑1 (MQ=60)

                                                         AACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAC                                  <  SRR4302540.1047926/94‑1 (MQ=60)

                                                          ACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACG                                 <  SRR4302540.2702559/94‑1 (MQ=60)

                                                            CAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTA                               >  SRR4302540.8934943/1‑94 (MQ=60)

                                                             AGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAC                              >  SRR4302540.6337931/1‑94 (MQ=60)

                                                              GAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACC                             <  SRR4302540.7548418/94‑1 (MQ=60)

                                                              GAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACC                             <  SRR4302540.678106/94‑1 (MQ=60)

                                                               AGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCA                            >  SRR4302540.1422200/1‑94 (MQ=60)

                                                               AGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCA                            <  SRR4302540.5986722/94‑1 (MQ=60)

                                                                GAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAT                           <  SRR4302540.1331594/94‑1 (MQ=60)

                                                                GAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAT                           >  SRR4302540.561606/1‑94 (MQ=60)

                                                                GAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAT                           >  SRR4302540.1917410/1‑94 (MQ=60)

                                                                GAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAT                           <  SRR4302540.2199659/94‑1 (MQ=60)

                                                                  ATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAC                         <  SRR4302540.9116310/94‑1 (MQ=60)

                                                                  ATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAC                         <  SRR4302540.3523984/94‑1 (MQ=60)

                                                                  ATATTCAGCGTTGATGCGACATCCAGATGGGCGGCGATAACGTACTCTTTTTCAAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAG                         >  SRR4302540.7095105/1‑94 (MQ=60)

                                                                   TATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACG                        <  SRR4302540.7733543/94‑1 (MQ=60)

                                                                   TATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACG                        >  SRR4302540.523107/1‑94 (MQ=60)

                                                                   TATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACG                        <  SRR4302540.8419747/94‑1 (MQ=60)

                                                                       CAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGC                    >  SRR4302540.9203990/1‑94 (MQ=60)

                                                                         GCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAG                  <  SRR4302540.5070310/94‑1 (MQ=60)

                                                                         GCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGCTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAG                  >  SRR4302540.7685369/1‑94 (MQ=60)

                                                                           GTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCA                >  SRR4302540.6446116/1‑94 (MQ=60)

                                                                           GTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCA                <  SRR4302540.850152/94‑1 (MQ=60)

                                                                           GTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCA                >  SRR4302540.8281590/1‑94 (MQ=60)

                                                                             TGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAAT              <  SRR4302540.5197877/94‑1 (MQ=60)

                                                                             TGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAAT              <  SRR4302540.8271400/94‑1 (MQ=60)

                                                                             TGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAAT              <  SRR4302540.8271401/94‑1 (MQ=60)

                                                                             TGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAAT              <  SRR4302540.603419/94‑1 (MQ=60)

                                                                             TGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAAT              <  SRR4302540.752452/94‑1 (MQ=60)

                                                                               ATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGC            >  SRR4302540.4073514/1‑94 (MQ=60)

                                                                                  ccaCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAG         <  SRR4302540.10047581/91‑1 (MQ=60)

                                                                                  CGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAG         <  SRR4302540.1594453/94‑1 (MQ=60)

                                                                                    CCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCC       >  SRR4302540.5018922/1‑94 (MQ=60)

                                                                                    CCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCC       >  SRR4302540.4700676/1‑94 (MQ=60)

                                                                                    CCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCC       >  SRR4302540.1096738/1‑94 (MQ=60)

                                                                                     CATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCA      >  SRR4302540.769084/1‑94 (MQ=60)

                                                                                     CATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCA      >  SRR4302540.1250479/1‑94 (MQ=60)

                                                                                     CATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCA      >  SRR4302540.7813272/1‑94 (MQ=60)

                                                                                      ATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAG     >  SRR4302540.5889523/1‑94 (MQ=60)

                                                                                      ATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAG     <  SRR4302540.9112051/94‑1 (MQ=60)

                                                                                      ATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAG     <  SRR4302540.8671354/94‑1 (MQ=60)

                                                                                        CCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAC   >  SRR4302540.1187992/1‑94 (MQ=60)

                                                                                         CAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  <  SRR4302540.5241595/94‑1 (MQ=60)

                                                                                         CAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  SRR4302540.9592665/1‑94 (MQ=60)

GACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  NC_000913/1351869‑1352051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`