Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,385,769	T→C	N342N (AAT→AAC)	tyrR →	TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,385,769	0	T	C	99.6%	847.4 / ‑5.6	265	N342N (AAT→AAC)	tyrR	TyrR
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (117/146); total (118/147)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 269 total aligned reads displayed.

GCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGA  >  NC_000913/1385679‑1385858

 ggTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTg                                                                                        <  2:7437119/92‑1 (MQ=255)

   tttCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGt                                                                                      <  1:1353224/93‑1 (MQ=255)

   tttCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGt                                                                                      <  1:8580520/93‑1 (MQ=255)

   tttCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGt                                                                                      >  2:1226732/1‑93 (MQ=255)

   tttCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGt                                                                                      >  2:165213/1‑93 (MQ=255)

   tttCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGt                                                                                      >  2:6975989/1‑93 (MQ=255)

   tttCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGt                                                                                      <  2:6451810/93‑1 (MQ=255)

     tCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCg                                                                                    >  2:492160/1‑93 (MQ=255)

      ccTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGa                                                                                   <  1:10043793/93‑1 (MQ=255)

      ccTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGa                                                                                   >  1:5558450/1‑93 (MQ=255)

        ttAATGATGGCGCTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAAc                                                                                 >  1:7213052/1‑93 (MQ=255)

        ttAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAAc                                                                                 <  2:3836662/93‑1 (MQ=255)

         tAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACt                                                                                >  2:4584743/1‑93 (MQ=255)

         tAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACt                                                                                >  2:8307059/1‑93 (MQ=255)

          aaTGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTg                                                                               >  2:9566238/1‑93 (MQ=255)

            tgatgGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGGGATTTGCGCTCCGCAGAAGAACCTGGTCGAACTggg                                                                             >  2:2908865/1‑92 (MQ=255)

              atgGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGc                                                                           <  1:3037378/93‑1 (MQ=255)

              atgGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGGGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGc                                                                           >  1:2696732/1‑93 (MQ=255)

               tgGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCa                                                                          >  2:9904394/1‑93 (MQ=255)

               tgGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCa                                                                          >  1:1083255/1‑93 (MQ=255)

                ggCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCaa                                                                         >  2:5443659/1‑93 (MQ=255)

                  cACTTTCCGTCGGGTTAGCGAAGACCATGAGGTGCATGTCGATGTGCGGGGGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCaaaa                                                                       >  2:5802892/1‑93 (MQ=255)

                   aCTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGCACTGGTGCaaaaa                                                                      >  1:3378091/1‑93 (MQ=255)

                   aCTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCaaaaa                                                                      >  1:9340565/1‑93 (MQ=255)

                   aCTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCaaaaa                                                                      <  2:3633666/93‑1 (MQ=255)

                   aCTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCaaaaa                                                                      <  1:5497382/93‑1 (MQ=255)

                     tttCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAgg                                                                    >  2:201666/1‑93 (MQ=255)

                     tttCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGCCGAACTGGGGCAAAAAgg                                                                    >  2:8005922/1‑93 (MQ=255)

                      ttCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGc                                                                   <  1:5879850/93‑1 (MQ=255)

                       tCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCa                                                                  <  1:7969132/93‑1 (MQ=255)

                       tCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCa                                                                  <  1:4520352/93‑1 (MQ=255)

                       tCCGTCGGGTTGGCGAAGACCATGAGGGGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCa                                                                  >  1:7103414/1‑93 (MQ=255)

                        ccGTCGGGTTGTCGCAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGACCCTGGTCGAACTGGTGCAAAAAGGCAt                                                                 <  1:8930715/93‑1 (MQ=255)

                          gTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGt                                                               >  2:4544386/1‑93 (MQ=255)

                          gTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGt                                                               <  2:849957/93‑1 (MQ=255)

                            cGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTc                                                             <  1:6097441/93‑1 (MQ=255)

                            cGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTc                                                             <  2:6059809/93‑1 (MQ=255)

                            cGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTc                                                             <  2:8680275/93‑1 (MQ=255)

                              ggTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCg                                                           <  1:3344755/93‑1 (MQ=255)

                              ggTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCg                                                           >  1:8469359/1‑93 (MQ=255)

                              ggTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCg                                                           >  2:2529813/1‑93 (MQ=255)

                               gTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCgg                                                          >  1:6830990/1‑92 (MQ=255)

                                ttGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTg                                                         >  2:681040/1‑93 (MQ=255)

                                   gCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAg                                                      <  2:9446658/93‑1 (MQ=255)

                                   gCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAg                                                      <  1:9643953/93‑1 (MQ=255)

                                     gAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGAt                                                    >  2:3591068/1‑93 (MQ=255)

                                         aCCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATctct                                                <  1:732915/93‑1 (MQ=255)

                                            aTGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatt                                             <  1:1525489/93‑1 (MQ=255)

                                            aTGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatt                                             <  2:5725747/93‑1 (MQ=255)

                                            aTGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGAAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatt                                             >  2:7759894/1‑93 (MQ=255)

                                             tGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatta                                            <  2:3993299/93‑1 (MQ=255)

                                             tGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatta                                            >  1:2090294/1‑93 (MQ=255)

                                             tGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatta                                            >  2:7079804/1‑93 (MQ=255)

                                             tGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatta                                            <  2:1390809/93‑1 (MQ=255)

                                             tGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtatta                                            >  2:1267561/1‑93 (MQ=255)

                                              gAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCtattat                                           <  1:4969214/93‑1 (MQ=255)

                                               tggTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGCACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATc                                          >  1:3144580/2‑93 (MQ=255)

                                                  tGCATGTCGATGTGCGGGTGCTTTGCGCTACGCAGAAGAATCTGGTCGCACTGGTGCAAAAAGGCATGTTCCGTGAAGGTCTCTATTATCGtt                                       >  1:3892393/1‑92 (MQ=255)

                                                  tGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTc                                       <  2:3047294/93‑1 (MQ=255)

                                                  tGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTc                                       <  2:2258225/93‑1 (MQ=255)

                                                   gCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCt                                      <  1:291167/93‑1 (MQ=255)

                                                   gCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCt                                      <  1:1863883/93‑1 (MQ=255)

                                                   gCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCt                                      >  1:5191626/1‑93 (MQ=255)

                                                    cATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTg                                     <  1:1410822/93‑1 (MQ=255)

                                                      tGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGaa                                   <  2:3347142/93‑1 (MQ=255)

                                                      tGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGaa                                   <  2:6501038/93‑1 (MQ=255)

                                                      tGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGaa                                   <  2:9182626/93‑1 (MQ=255)

                                                      tGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGaa                                   <  2:975861/93‑1 (MQ=255)

                                                        tCGATGTGCGGGCGATTTGCGCTACGCAGCAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGACCg                                 >  1:4069305/1‑93 (MQ=255)

                                                          gATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACgtg                               <  1:228815/93‑1 (MQ=255)

                                                            tgtgCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGtt                             >  1:7584724/1‑93 (MQ=255)

                                                            tgtgCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACGGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGtt                             <  2:2369970/93‑1 (MQ=255)

                                                             gtgCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTg                            <  2:5598127/93‑1 (MQ=255)

                                                              tgCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGa                           <  1:6596338/93‑1 (MQ=255)

                                                              tgCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGa                           <  1:3049512/93‑1 (MQ=255)

                                                                cGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACg                         <  2:3869996/93‑1 (MQ=255)

                                                                   gTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTc                      >  2:5130347/1‑93 (MQ=255)

                                                                   gTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTc                      <  2:5278326/93‑1 (MQ=255)

                                                                     gATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCaa                    >  1:5481203/1‑93 (MQ=255)

                                                                     gATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCaa                    >  1:3663790/1‑93 (MQ=255)

                                                                       tttGTGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATc                  <  2:1069763/93‑1 (MQ=255)

                                                                       tttGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATc                  <  2:8386234/93‑1 (MQ=255)

                                                                         tGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTg                >  2:5891654/1‑93 (MQ=255)

                                                                         tGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGGCTGAACGTGTTGACGCTCAATCTg                >  2:2167708/1‑93 (MQ=255)

                                                                          gcgcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgc               <  1:8673353/93‑1 (MQ=255)

                                                                          gcgcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgc               <  1:7019577/93‑1 (MQ=255)

                                                                           cgcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgcc              <  1:1588722/93‑1 (MQ=255)

                                                                           cgcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgcc              <  1:2614698/93‑1 (MQ=255)

                                                                           cgcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgcc              <  2:7558482/93‑1 (MQ=255)

                                                                           cgcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgcc              >  1:3832489/1‑93 (MQ=255)

                                                                            gcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgccg             >  1:5788682/1‑93 (MQ=255)

                                                                            gcTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTgccg             <  2:3697662/93‑1 (MQ=255)

                                                                                cGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCcgccgc         <  1:492160/93‑1 (MQ=255)

                                                                                 gCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCt        >  1:6375095/1‑93 (MQ=255)

                                                                                  cAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTa       <  1:5405896/93‑1 (MQ=255)

                                                                                    gaagaaCCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACg     >  1:9773908/1‑93 (MQ=255)

                                                                                    gaagaaCCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACg     <  1:6253043/93‑1 (MQ=255)

                                                                                     aagaaCCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGt    <  2:1847680/93‑1 (MQ=255)

                                                                                      agaaCCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGCTGACGCTCAATCTGCCGCCGCTACGTg   >  2:8879205/1‑93 (MQ=255)

                                                                                       gaaCCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGa  <  1:769855/93‑1 (MQ=255)

GCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGA  >  NC_000913/1385679‑1385858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT  >  NC_000913/1385676‑1385862

ACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGGTTTGCGCTACGCAGAAGAAC                                                                                               >  SRR4302540.9244235/1‑94 (MQ=60)

ACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAAC                                                                                               >  SRR4302540.3665052/1‑94 (MQ=60)

ACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAAC                                                                                               <  SRR4302540.4063503/94‑1 (MQ=60)

ACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAAC                                                                                               >  SRR4302540.1997992/1‑94 (MQ=60)

 CTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACC                                                                                              <  SRR4302540.1202632/94‑1 (MQ=60)

 CTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGNGNTTTGCGCTACNCANANGAACC                                                                                              >  SRR4302540.9928700/1‑94 (MQ=60)

  TGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCT                                                                                             <  SRR4302540.241283/94‑1 (MQ=60)

  TGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCT                                                                                             <  SRR4302540.4807255/94‑1 (MQ=60)

   GCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTG                                                                                            <  SRR4302540.3414384/94‑1 (MQ=60)

   GCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTG                                                                                            <  SRR4302540.5761181/94‑1 (MQ=60)

    CGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGG                                                                                           >  SRR4302540.4217027/1‑94 (MQ=60)

      TTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTC                                                                                         <  SRR4302540.8680266/94‑1 (MQ=60)

      TTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTC                                                                                         <  SRR4302540.1367465/94‑1 (MQ=60)

      TTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTC                                                                                         >  SRR4302540.6241877/1‑94 (MQ=60)

      TTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTC                                                                                         >  SRR4302540.3902943/1‑94 (MQ=60)

      TTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTC                                                                                         <  SRR4302540.6765257/94‑1 (MQ=60)

        TCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGA                                                                                       >  SRR4302540.5619670/1‑94 (MQ=60)

         CCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAA                                                                                      <  SRR4302540.10160562/94‑1 (MQ=60)

          CTTAATGATGGCGCTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAAC                                                                                     >  SRR4302540.7293460/1‑94 (MQ=60)

          CTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAAC                                                                                     <  SRR4302540.7102226/94‑1 (MQ=60)

          CTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAAC                                                                                     <  SRR4302540.8726384/94‑1 (MQ=60)

           TTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACT                                                                                    >  SRR4302540.4752464/1‑94 (MQ=60)

              ATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGT                                                                                 <  SRR4302540.7522171/94‑1 (MQ=60)

              ATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGG                                                                                 >  SRR4302540.7770358/1‑94 (MQ=60)

                GATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGC                                                                               >  SRR4302540.7091224/1‑94 (MQ=60)

                GATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGC                                                                               <  SRR4302540.7743913/94‑1 (MQ=60)

                GATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGGGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGC                                                                               >  SRR4302540.2725839/1‑94 (MQ=60)

                 ATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCA                                                                              >  SRR4302540.1094363/1‑94 (MQ=60)

                 ATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCA                                                                              <  SRR4302540.555531/94‑1 (MQ=60)

                 ATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCA                                                                              <  SRR4302540.3070718/94‑1 (MQ=60)

                   GGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAA                                                                            <  SRR4302540.4329098/94‑1 (MQ=60)

                    GCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAA                                                                           >  SRR4302540.305015/1‑94 (MQ=60)

                    GCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAA                                                                           <  SRR4302540.6862350/94‑1 (MQ=60)

                     CACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGCACTGGTGCAAAAA                                                                          >  SRR4302540.3414986/1‑94 (MQ=60)

                     CACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAA                                                                          >  SRR4302540.9451879/1‑94 (MQ=60)

                      ACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAG                                                                         <  SRR4302540.5557912/94‑1 (MQ=60)

                      ACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAG                                                                         <  SRR4302540.5557914/94‑1 (MQ=60)

                       CTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGG                                                                        >  SRR4302540.7321207/1‑94 (MQ=60)

                         TTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCA                                                                      <  SRR4302540.5944832/94‑1 (MQ=60)

                         TTCCGTCGGGTTGGCGAAGACCATGAGGGGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCA                                                                      >  SRR4302540.7182448/1‑94 (MQ=60)

                          TCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCAT                                                                     >  SRR4302540.6362530/1‑94 (MQ=60)

                          TCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCAT                                                                     <  SRR4302540.9907221/94‑1 (MQ=60)

                          TCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCAT                                                                     <  SRR4302540.4569310/94‑1 (MQ=60)

                          TCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCAT                                                                     <  SRR4302540.8061993/94‑1 (MQ=60)

                           CCGTCGGGTTGTCGCAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGACCCTGGTCGAACTGGTGCAAAAAGGCATG                                                                    <  SRR4302540.9034910/94‑1 (MQ=60)

                           CCGTCGGGTTGGCGACGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATG                                                                    <  SRR4302540.9034909/94‑1 (MQ=60)

                            CGTCGGGTTGGCTAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGT                                                                   <  SRR4302540.6040399/94‑1 (MQ=60)

                               CGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCC                                                                <  SRR4302540.5193121/94‑1 (MQ=60)

                               CGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCC                                                                <  SRR4302540.6165316/94‑1 (MQ=60)

                               CGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCC                                                                <  SRR4302540.2845261/94‑1 (MQ=60)

                                GGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCG                                                               >  SRR4302540.8567705/1‑94 (MQ=60)

                                GGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCG                                                               <  SRR4302540.1533425/94‑1 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGATCTGGTGCAAAAAGGCATGTTCCGT                                                              <  SRR4302540.5526582/94‑1 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGT                                                              <  SRR4302540.5805900/94‑1 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGT                                                              <  SRR4302540.8360403/94‑1 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGT                                                              >  SRR4302540.6572730/1‑94 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGT                                                              >  SRR4302540.3176585/1‑94 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGT                                                              <  SRR4302540.3381451/94‑1 (MQ=60)

                                 GGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGG                                                              >  SRR4302540.6906626/1‑94 (MQ=60)

                                  GTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTG                                                             <  SRR4302540.1185412/94‑1 (MQ=60)

                                    TGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAA                                                           <  SRR4302540.4111219/94‑1 (MQ=60)

                                    TGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAA                                                           >  SRR4302540.1908046/1‑94 (MQ=60)

                                      GCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGA                                                         <  SRR4302540.9859143/94‑1 (MQ=60)

                                      GCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGA                                                         <  SRR4302540.9757540/94‑1 (MQ=60)

                                       CGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGAT                                                        >  SRR4302540.3160593/1‑94 (MQ=60)

                                        GAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATC                                                       >  SRR4302540.5617386/1‑94 (MQ=60)

                                          AGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTC                                                     <  SRR4302540.5069423/94‑1 (MQ=60)

                                           AACCATGGGGTGCATGTCGATGTGCGGGCGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCT                                                    <  SRR4302540.3181982/94‑1 (MQ=60)

                                            ACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTA                                                   <  SRR4302540.740754/94‑1 (MQ=60)

                                            ACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTA                                                   <  SRR4302540.740752/94‑1 (MQ=60)

                                             CCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTAT                                                  <  SRR4302540.1604651/94‑1 (MQ=60)

                                             CCATGAGGTGCATGTCGATGTGCGGGTGATGTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTAT                                                  >  SRR4302540.3818494/1‑94 (MQ=60)

                                              CATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATT                                                 <  SRR4302540.388257/94‑1 (MQ=60)

                                               ATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAACGGCATGTTCCGTGAAGATCTCTATTA                                                >  SRR4302540.5324379/1‑94 (MQ=60)

                                               ATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTA                                                <  SRR4302540.1542383/94‑1 (MQ=60)

                                               ATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTA                                                >  SRR4302540.2112747/1‑94 (MQ=60)

                                                 GTGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGCACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATC                                              >  SRR4302540.3178849/1‑94 (MQ=60)

                                                 GAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATC                                              <  SRR4302540.2996124/94‑1 (MQ=60)

                                                 GAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATC                                              <  SRR4302540.5022979/94‑1 (MQ=60)

                                                   GGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGT                                            >  SRR4302540.863462/1‑94 (MQ=60)

                                                    GTGCATGTCGATGTGCGGGTGCTTTGCGCTACGCAGAAGAATCTGGTCGCACTGGTGCAAAAAGGCATGTTCCGTGAAGGTCTCTATTATCGTT                                           >  SRR4302540.3934649/1‑94 (MQ=60)

                                                    GTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTC                                           >  SRR4302540.3872831/1‑94 (MQ=60)

                                                     TGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCT                                          >  SRR4302540.5248156/1‑94 (MQ=60)

                                                     TGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCT                                          >  SRR4302540.1698738/1‑94 (MQ=60)

                                                     TGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCT                                          <  SRR4302540.6163027/94‑1 (MQ=60)

                                                      GCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTG                                         <  SRR4302540.1884315/94‑1 (MQ=60)

                                                      GCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTG                                         <  SRR4302540.294046/94‑1 (MQ=60)

                                                       CATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGA                                        <  SRR4302540.1426030/94‑1 (MQ=60)

                                                       CATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGA                                        >  SRR4302540.5932362/1‑94 (MQ=60)

                                                       CATGTCGATGTGCGGGTGAGTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGA                                        <  SRR4302540.1136901/94‑1 (MQ=60)

                                                        ATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAA                                       >  SRR4302540.1387554/1‑94 (MQ=60)

                                                        ATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAA                                       >  SRR4302540.1387555/1‑94 (MQ=60)

                                                        ATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAA                                       >  SRR4302540.1794619/1‑94 (MQ=60)

                                                          GTCGATGTGCGGGCGATTTGCGCTACGCAGCAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGACCG                                     >  SRR4302540.4113170/1‑94 (MQ=60)

                                                            CGATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTG                                   <  SRR4302540.2279160/94‑1 (MQ=60)

                                                             GATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGT                                  >  SRR4302540.5013870/1‑94 (MQ=60)

                                                             GATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGT                                  <  SRR4302540.231028/94‑1 (MQ=60)

                                                             GATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGATCCGTGAAGATCTATCTTATCGTCTGAACGTAT                                  >  SRR4302540.5013871/1‑94 (MQ=60)

                                                              ATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTT                                 <  SRR4302540.6656486/94‑1 (MQ=60)

                                                              ATGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTT                                 >  SRR4302540.7669878/1‑94 (MQ=60)

                                                               TGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTG                                >  SRR4302540.9638641/1‑94 (MQ=60)

                                                               TGTGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGGGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGACAGTGTTG                                >  SRR4302540.5259674/1‑94 (MQ=60)

                                                                 TGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGAC                              <  SRR4302540.6669384/94‑1 (MQ=60)

                                                                 TGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGAC                              <  SRR4302540.2288277/94‑1 (MQ=60)

                                                                 TGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGAC                              <  SRR4302540.3082964/94‑1 (MQ=60)

                                                                 TGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGAC                              <  SRR4302540.285844/94‑1 (MQ=60)

                                                                 TGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGAC                              <  SRR4302540.8652055/94‑1 (MQ=60)

                                                                    GGGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCT                           >  SRR4302540.7230806/1‑94 (MQ=60)

                                                                     GGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTC                          >  SRR4302540.1568460/1‑94 (MQ=60)

                                                                     GGTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTA                          >  SRR4302540.2415112/1‑94 (MQ=60)

                                                                      GTGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCA                         >  SRR4302540.6261554/1‑94 (MQ=60)

                                                                       TGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAA                        >  SRR4302540.4690048/1‑94 (MQ=60)

                                                                       TGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAA                        >  SRR4302540.5541536/1‑94 (MQ=60)

                                                                       TGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAA                        >  SRR4302540.3401761/1‑94 (MQ=60)

                                                                       TGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAA                        >  SRR4302540.9339735/1‑94 (MQ=60)

                                                                       TGATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAA                        >  SRR4302540.3703889/1‑94 (MQ=60)

                                                                        GATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAAT                       >  SRR4302540.2509902/1‑94 (MQ=60)

                                                                         ATTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATC                      >  SRR4302540.3535132/1‑94 (MQ=60)

                                                                          TTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCT                     <  SRR4302540.950393/94‑1 (MQ=60)

                                                                          TTTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCT                     <  SRR4302540.7989046/94‑1 (MQ=60)

                                                                           TTGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTG                    <  SRR4302540.7104396/94‑1 (MQ=60)

                                                                            TGCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGC                   >  SRR4302540.3072115/1‑94 (MQ=60)

                                                                             GCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCC                  <  SRR4302540.7097541/94‑1 (MQ=60)

                                                                             GCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCC                  <  SRR4302540.8774099/94‑1 (MQ=60)

                                                                             GCGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCC                  >  SRR4302540.3874195/1‑94 (MQ=60)

                                                                              tcCTACGCAGAAGAACCTGGGCGAACTTGTGCAAAAAGGCATGGTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACCCTCAATCTGCCG                 <  SRR4302540.2642946/92‑1 (MQ=60)

                                                                              CGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCG                 <  SRR4302540.1063085/94‑1 (MQ=60)

                                                                              CGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCG                 <  SRR4302540.1606078/94‑1 (MQ=60)

                                                                              CGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCG                 <  SRR4302540.2642940/94‑1 (MQ=60)

                                                                              CGCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCG                 >  SRR4302540.5852640/1‑94 (MQ=60)

                                                                              CGCTACGCAGAAGAACCTGGTCGAAATGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCG                 <  SRR4302540.3228482/94‑1 (MQ=60)

                                                                               GCTACGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGC                >  SRR4302540.718326/1‑94 (MQ=60)

                                                                                   CGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCT            <  SRR4302540.497429/94‑1 (MQ=60)

                                                                                   CGCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCT            >  SRR4302540.6446074/1‑94 (MQ=60)

                                                                                    GCAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTA           <  SRR4302540.8733954/94‑1 (MQ=60)

                                                                                     CAGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTAC          <  SRR4302540.5465337/94‑1 (MQ=60)

                                                                                      AGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACG         >  SRR4302540.9888488/1‑94 (MQ=60)

                                                                                      AGAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACG         >  SRR4302540.5551518/1‑94 (MQ=60)

                                                                                       GAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGT        <  SRR4302540.6322617/94‑1 (MQ=60)

                                                                                       GAAGAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGT        <  SRR4302540.9226326/94‑1 (MQ=60)

                                                                                          GAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGAC     <  SRR4302540.778049/94‑1 (MQ=60)

                                                                                          GAACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGAC     <  SRR4302540.8436540/94‑1 (MQ=60)

                                                                                           AACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACT    <  SRR4302540.5725651/94‑1 (MQ=60)

                                                                                           AACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACT    <  SRR4302540.6800723/94‑1 (MQ=60)

                                                                                           AACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACT    >  SRR4302540.345586/1‑94 (MQ=60)

                                                                                           AACCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACG    >  SRR4302540.1920349/1‑94 (MQ=60)

                                                                                             CCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGATCGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT  <  SRR4302540.3945859/94‑1 (MQ=60)

ACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT  >  NC_000913/1385676‑1385862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`