Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,126	T→C	M252V (ATG→GTG)	b1341 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,126	0	T	C	99.7%	1002.3 / ‑5.3	310	M252V (ATG→GTG)	b1341	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (164/145); total (164/146)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 322 total aligned reads displayed.

GTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACATGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG  >  NC_000913/1405036‑1405216

gTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGa                                                                                          <  2:7877286/93‑1 (MQ=255)

gTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGa                                                                                          >  1:5644442/1‑93 (MQ=255)

 tAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAg                                                                                         >  2:100715/1‑93 (MQ=255)

  aaTCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGc                                                                                        >  1:7024504/1‑93 (MQ=255)

   aTCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCt                                                                                       <  1:3637790/93‑1 (MQ=255)

   aTCCGGTCATCGCGTAATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCt                                                                                       <  2:4260097/93‑1 (MQ=255)

    tCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCtt                                                                                      >  1:1226079/1‑93 (MQ=255)

     ccGGTCATCGCGTCATGGTGAGCAGCTTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTg                                                                                     <  2:9333601/93‑1 (MQ=255)

     ccGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTg                                                                                     >  2:2513126/1‑93 (MQ=255)

         tCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGc                                                                                 >  1:7459601/1‑93 (MQ=255)

         tCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGc                                                                                 >  2:6416845/1‑93 (MQ=255)

           aTCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCa                                                                               >  2:5078087/1‑93 (MQ=255)

            tCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCAt                                                                              >  2:3874423/1‑93 (MQ=255)

            tCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCAt                                                                              >  1:353823/1‑93 (MQ=255)

             cgcgTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATa                                                                             <  2:2556989/93‑1 (MQ=255)

              gcgTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATaa                                                                            >  1:9968309/1‑93 (MQ=255)

               cgTCATGGTGAGCAGCATGTTCCTGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATaaa                                                                           <  2:7231859/93‑1 (MQ=255)

                   aTGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTc                                                                       >  1:4413211/1‑93 (MQ=255)

                     ggTTAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCaa                                                                     <  2:3979563/93‑1 (MQ=255)

                     ggTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCaa                                                                     <  2:1837436/93‑1 (MQ=255)

                       tGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATc                                                                   >  2:9638511/1‑93 (MQ=255)

                          gcagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGt                                                                >  2:862096/1‑93 (MQ=255)

                          gcagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGt                                                                >  2:9217152/1‑93 (MQ=255)

                          gcagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGt                                                                >  1:2634546/1‑93 (MQ=255)

                           cagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTc                                                               <  2:5993346/93‑1 (MQ=255)

                           cagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTc                                                               >  2:129001/1‑93 (MQ=255)

                           cagcaTGTTCCAGCTGCTCCTCCAGCCGTTGTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTGACTCGGTc                                                               >  1:2952093/1‑93 (MQ=255)

                             gcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATACATTTCAATCGGTCCg                                                             >  1:2199284/1‑93 (MQ=255)

                             gcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCg                                                             >  1:6691997/1‑93 (MQ=255)

                             gcaTGTTACAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCg                                                             <  1:9296091/93‑1 (MQ=255)

                              caTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCgg                                                            <  2:55696/93‑1 (MQ=255)

                               aTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGc                                                           >  1:6019389/1‑93 (MQ=255)

                               aTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGc                                                           <  2:3941292/93‑1 (MQ=255)

                                tGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          >  2:6717024/1‑93 (MQ=255)

                                tGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          <  2:569297/93‑1 (MQ=255)

                                tGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          >  1:7631075/1‑93 (MQ=255)

                                tGTTCCAGCTGCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          <  1:4960078/93‑1 (MQ=255)

                                  ttCCAGCTCCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATa                                                        <  1:3758922/93‑1 (MQ=255)

                                    ccAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAgg                                                      >  1:7706273/1‑93 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    <  1:7969664/93‑1 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    <  2:7400622/93‑1 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    <  1:9490080/93‑1 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    >  2:6527282/1‑93 (MQ=255)

                                         tgctCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGc                                                 <  2:5835953/93‑1 (MQ=255)

                                         tgctCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGc                                                 <  2:6135418/93‑1 (MQ=255)

                                         tgctCCCCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGc                                                 <  2:4106644/93‑1 (MQ=255)

                                          gctCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGca                                                >  2:1503361/1‑93 (MQ=255)

                                            tcctccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGcaca                                              <  2:8226211/93‑1 (MQ=255)

                                            ccctccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGcaca                                              <  1:3380384/92‑1 (MQ=255)

                                             cctccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACAt                                             >  1:2011377/1‑93 (MQ=255)

                                             catccAGCCTTTTTTGCTCAGTAATATCAGGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACAt                                             <  2:4408778/91‑1 (MQ=255)

                                               tccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGa                                           >  2:287896/1‑93 (MQ=255)

                                               tccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGa                                           >  1:2357935/1‑93 (MQ=255)

                                                ccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAc                                          >  2:7806126/1‑93 (MQ=255)

                                                ccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAc                                          >  1:6842207/1‑93 (MQ=255)

                                                ccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAc                                          >  2:2768285/1‑93 (MQ=255)

                                                  aGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGa                                        >  1:8684959/1‑93 (MQ=255)

                                                  aGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGa                                        >  1:6522220/1‑93 (MQ=255)

                                                   gCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAg                                       <  2:2158377/93‑1 (MQ=255)

                                                   gCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAg                                       >  1:4564354/1‑93 (MQ=255)

                                                   gCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAg                                       <  2:1354923/93‑1 (MQ=255)

                                                     cGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTc                                     <  2:4186865/93‑1 (MQ=255)

                                                      gTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCg                                    >  2:9848230/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  1:5199345/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  1:2511042/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  2:6168843/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  1:9713594/1‑93 (MQ=255)

                                                         tttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAc                                 <  1:4830352/93‑1 (MQ=255)

                                                         tttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAc                                 <  1:6371571/93‑1 (MQ=255)

                                                          ttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAcc                                >  2:5301185/1‑93 (MQ=255)

                                                          ttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAcc                                <  2:3067685/93‑1 (MQ=255)

                                                           tGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCa                               <  2:4706254/93‑1 (MQ=255)

                                                            gCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAt                              <  1:3181402/93‑1 (MQ=255)

                                                             cTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATc                             >  2:3286296/1‑93 (MQ=255)

                                                              tCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATcc                            >  2:8336785/1‑93 (MQ=255)

                                                               cAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCg                           >  1:1577695/1‑93 (MQ=255)

                                                                 gTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGcc                         >  2:9132436/1‑93 (MQ=255)

                                                                   aaTATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAg                       >  1:8461853/1‑93 (MQ=255)

                                                                   aaTATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAg                       >  1:307967/1‑93 (MQ=255)

                                                                    atatCAAGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGt                      >  2:3116039/1‑93 (MQ=255)

                                                                     tatCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGtt                     <  2:3799342/93‑1 (MQ=255)

                                                                     tatCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGtt                     >  2:4886549/1‑93 (MQ=255)

                                                                        cATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTAt                  <  1:2806376/93‑1 (MQ=255)

                                                                        cATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTAt                  >  1:1278690/1‑93 (MQ=255)

                                                                         aTGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATg                 <  1:1007499/93‑1 (MQ=255)

                                                                             acaATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACa             <  1:7839234/93‑1 (MQ=255)

                                                                             acaATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACa             >  2:899285/1‑93 (MQ=255)

                                                                              caATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACaa            >  1:6599213/1‑93 (MQ=255)

                                                                               aaTACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACaaa           <  1:573769/93‑1 (MQ=255)

                                                                                aTACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACaaaa          >  2:9240149/1‑93 (MQ=255)

                                                                                aTACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAGCCGCCAGTTTATGAACaaaa          <  2:9683861/93‑1 (MQ=255)

                                                                                 tACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAAt         >  2:7711089/1‑93 (MQ=255)

                                                                                   cATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAACATTc       >  2:8125229/1‑93 (MQ=255)

                                                                                   cATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTc       >  1:1423256/1‑93 (MQ=255)

                                                                                    aTAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCa      >  2:6987416/1‑93 (MQ=255)

                                                                                    aTAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCa      <  1:3975154/93‑1 (MQ=255)

                                                                                     tAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCaa     >  1:8094385/1‑93 (MQ=255)

                                                                                      aaCACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCaaa    <  2:9775617/93‑1 (MQ=255)

                                                                                       acacGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAg   <  1:5480593/93‑1 (MQ=255)

                                                                                       acacGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAg   >  1:9028438/1‑93 (MQ=255)

GTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACATGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG  >  NC_000913/1405036‑1405216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACATGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGGTTT  >  NC_000913/1405033‑1405219

TCAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACAC                                                                                               <  SRR4302540.7631186/94‑1 (MQ=60)

TCAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACAC                                                                                               <  SRR4302540.5793665/94‑1 (MQ=60)

TCAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACAC                                                                                               <  SRR4302540.1547995/94‑1 (MQ=60)

 CAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACG                                                                                              >  SRR4302540.2902528/1‑94 (MQ=60)

 CAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACG                                                                                              >  SRR4302540.1099562/1‑94 (MQ=60)

  AGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGA                                                                                             <  SRR4302540.3136765/94‑1 (MQ=60)

  AGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGA                                                                                             >  SRR4302540.5706563/1‑94 (MQ=60)

   GTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAG                                                                                            <  SRR4302540.7756506/94‑1 (MQ=60)

   GTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAG                                                                                            <  SRR4302540.5693040/94‑1 (MQ=60)

    TAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGC                                                                                           <  SRR4302540.2762135/94‑1 (MQ=60)

    TAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGC                                                                                           >  SRR4302540.7102535/1‑94 (MQ=60)

     AATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCT                                                                                          >  SRR4302540.2479591/1‑94 (MQ=60)

      ATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTT                                                                                         >  SRR4302540.1238611/1‑94 (MQ=60)

      ATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTT                                                                                         <  SRR4302540.3677618/94‑1 (MQ=60)

        ccgggcatcgtgtcatggtcgttgtCATGTTCCAGCTGCTCCGCCAGCCTTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGT                                                                                       <  SRR4302540.8616208/69‑1 (MQ=60)

        CCGCTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGT                                                                                       <  SRR4302540.281549/94‑1 (MQ=60)

         CGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTC                                                                                      <  SRR4302540.5782024/94‑1 (MQ=60)

         CGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTC                                                                                      >  SRR4302540.6583692/1‑94 (MQ=60)

         CGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTC                                                                                      <  SRR4302540.3086755/94‑1 (MQ=60)

          GGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCG                                                                                     <  SRR4302540.4519664/94‑1 (MQ=60)

          GGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCG                                                                                     >  SRR4302540.2897819/1‑94 (MQ=60)

          GGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCG                                                                                     >  SRR4302540.3507010/1‑94 (MQ=60)

           GTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGC                                                                                    >  SRR4302540.7542776/1‑94 (MQ=60)

           GTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGC                                                                                    >  SRR4302540.114450/1‑94 (MQ=60)

             CATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCA                                                                                  <  SRR4302540.2236087/94‑1 (MQ=60)

              ATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCAT                                                                                 >  SRR4302540.357451/1‑94 (MQ=60)

                CGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAA                                                                               >  SRR4302540.10084599/1‑94 (MQ=60)

                 GCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAA                                                                              >  SRR4302540.3748771/1‑94 (MQ=60)

                  CGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAAT                                                                             <  SRR4302540.4581587/94‑1 (MQ=60)

                   GTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATT                                                                            >  SRR4302540.1444536/1‑94 (MQ=60)

                    TCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTT                                                                           >  SRR4302540.64843/1‑94 (MQ=60)

                     CATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTC                                                                          >  SRR4302540.4461043/1‑94 (MQ=60)

                     CATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTC                                                                          >  SRR4302540.2632070/1‑94 (MQ=60)

                       TGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAA                                                                        >  SRR4302540.662563/1‑94 (MQ=60)

                         GTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATC                                                                      <  SRR4302540.5473004/94‑1 (MQ=60)

                         GTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATC                                                                      <  SRR4302540.4804306/94‑1 (MQ=60)

                          TGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCG                                                                     <  SRR4302540.4301910/94‑1 (MQ=60)

                           GAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGG                                                                    >  SRR4302540.6989762/1‑94 (MQ=60)

                           GAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGG                                                                    <  SRR4302540.2411935/94‑1 (MQ=60)

                            AGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGT                                                                   >  SRR4302540.2663073/1‑94 (MQ=60)

                             GCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTC                                                                  >  SRR4302540.2984594/1‑94 (MQ=60)

                             GCAGCATGTTCCAGCTGCTCCTCCAGCCGTTGTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTGACTCGGTC                                                                  >  SRR4302540.2984595/1‑94 (MQ=60)

                              CAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCC                                                                 >  SRR4302540.8480171/1‑94 (MQ=60)

                               AGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATACATTTCAATCGGTCCG                                                                >  SRR4302540.2223034/1‑94 (MQ=60)

                               AGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCG                                                                >  SRR4302540.6765882/1‑94 (MQ=60)

                               AGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCG                                                                >  SRR4302540.3600617/1‑94 (MQ=60)

                               AGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCG                                                                >  SRR4302540.3600616/1‑94 (MQ=60)

                               AGCATGTTCCAGCTGCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCG                                                                <  SRR4302540.8780032/94‑1 (MQ=60)

                                GCATGTTACAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGG                                                               <  SRR4302540.9407156/94‑1 (MQ=60)

                                 CATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGC                                                              >  SRR4302540.6086155/1‑94 (MQ=60)

                                 CATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGC                                                              >  SRR4302540.6086153/1‑94 (MQ=60)

                                  ATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCA                                                             >  SRR4302540.7716913/1‑94 (MQ=60)

                                   TGTTCCAGCTGCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCAT                                                            <  SRR4302540.5013705/94‑1 (MQ=60)

                                     TTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAG                                                          >  SRR4302540.3427592/1‑94 (MQ=60)

                                     TTCCAGCTCCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAG                                                          <  SRR4302540.3799926/94‑1 (MQ=60)

                                     GTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAG                                                          <  SRR4302540.5136542/94‑1 (MQ=60)

                                      TCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGG                                                         >  SRR4302540.7794589/1‑94 (MQ=60)

                                       CCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGT                                                        >  SRR4302540.4383603/1‑94 (MQ=60)

                                         AGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCT                                                      <  SRR4302540.9602583/94‑1 (MQ=60)

                                         AGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCT                                                      <  SRR4302540.8062532/94‑1 (MQ=60)

                                          GCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTG                                                     <  SRR4302540.1540001/94‑1 (MQ=60)

                                            TGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCA                                                   <  SRR4302540.9158474/94‑1 (MQ=60)

                                             GCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCAC                                                  <  SRR4302540.4641903/94‑1 (MQ=60)

                                              CTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACA                                                 >  SRR4302540.5965953/1‑94 (MQ=60)

                                              CTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACA                                                 >  SRR4302540.795456/1‑94 (MQ=60)

                                               TCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACAT                                                >  SRR4302540.2033130/1‑94 (MQ=60)

                                               CCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACAT                                                <  SRR4302540.3417293/94‑1 (MQ=60)

                                                 CTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGC                                              >  SRR4302540.6134939/1‑94 (MQ=60)

                                                 CTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGA                                              >  SRR4302540.2383332/1‑94 (MQ=60)

                                                 CTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGA                                              <  SRR4302540.2787206/94‑1 (MQ=60)

                                                 CTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGA                                              <  SRR4302540.4982127/94‑1 (MQ=60)

                                                 CTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGA                                              <  SRR4302540.1949070/94‑1 (MQ=60)

                                                 CTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGA                                              >  SRR4302540.5914824/1‑94 (MQ=60)

                                                  TCCCGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAC                                             <  SRR4302540.122713/94‑1 (MQ=60)

                                                  TCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAC                                             >  SRR4302540.6917997/1‑94 (MQ=60)

                                                  CCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAC                                             <  SRR4302540.2067862/94‑1 (MQ=60)

                                                    CAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGA                                           >  SRR4302540.8785808/1‑94 (MQ=60)

                                                    CAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGA                                           >  SRR4302540.7305451/1‑94 (MQ=60)

                                                    CAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGA                                           >  SRR4302540.6594657/1‑94 (MQ=60)

                                                     AGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAG                                          >  SRR4302540.7611715/1‑94 (MQ=60)

                                                     AGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAG                                          >  SRR4302540.1675782/1‑94 (MQ=60)

                                                     AGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAG                                          >  SRR4302540.4613649/1‑94 (MQ=60)

                                                     AGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAG                                          >  SRR4302540.4882180/1‑94 (MQ=60)

                                                     AGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAG                                          <  SRR4302540.5687207/94‑1 (MQ=60)

                                                        CGTTTTTGCTCTGTAATATCTTGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGTCATATGTCTGCACATGACGAGTCG                                       >  SRR4302540.9074937/1‑94 (MQ=60)

                                                        CGTTGTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCG                                       <  SRR4302540.3946897/94‑1 (MQ=60)

                                                         GTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGA                                      >  SRR4302540.2538164/1‑94 (MQ=60)

                                                         GTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGA                                      >  SRR4302540.9827741/1‑94 (MQ=60)

                                                         GTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGA                                      <  SRR4302540.9640780/94‑1 (MQ=60)

                                                         GTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGA                                      >  SRR4302540.4373107/1‑94 (MQ=60)

                                                         GTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGA                                      >  SRR4302540.6865093/1‑94 (MQ=60)

                                                         GTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGA                                      >  SRR4302540.5255968/1‑94 (MQ=60)

                                                           TTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAC                                    <  SRR4302540.7960846/94‑1 (MQ=60)

                                                            TTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACC                                   <  SRR4302540.4882088/94‑1 (MQ=60)

                                                            TTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACC                                   <  SRR4302540.6442517/94‑1 (MQ=60)

                                                              TGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAT                                 >  SRR4302540.3171808/1‑94 (MQ=60)

                                                              TGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAT                                 <  SRR4302540.1380050/94‑1 (MQ=60)

                                                              TGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAT                                 <  SRR4302540.9477448/94‑1 (MQ=60)

                                                               GCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATC                                <  SRR4302540.3215967/94‑1 (MQ=60)

                                                                CTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCC                               >  SRR4302540.7777299/1‑94 (MQ=60)

                                                                 TCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCG                              >  SRR4302540.1594950/1‑94 (MQ=60)

                                                                 TCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCG                              >  SRR4302540.3379645/1‑94 (MQ=60)

                                                                 TCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCG                              >  SRR4302540.8073581/1‑94 (MQ=60)

                                                                   AGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCC                            <  SRR4302540.9108208/94‑1 (MQ=60)

                                                                   AGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCC                            >  SRR4302540.1006569/1‑94 (MQ=60)

                                                                   AGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCC                            >  SRR4302540.10019243/1‑94 (MQ=60)

                                                                   AGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCC                            >  SRR4302540.3205096/1‑94 (MQ=60)

                                                                    GTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGCCGAGTCGAACCATCCGCCA                           >  SRR4302540.6997345/1‑94 (MQ=60)

                                                                    GTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCA                           >  SRR4302540.8092511/1‑94 (MQ=60)

                                                                    GTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCA                           >  SRR4302540.2291230/1‑94 (MQ=60)

                                                                     TTATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTACGGCATAGGTCTGCACATGACGCGTCGAACCATCCGCCCG                          >  SRR4302540.8560110/1‑94 (MQ=60)

                                                                     TAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAG                          >  SRR4302540.311024/1‑94 (MQ=60)

                                                                     TAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAG                          >  SRR4302540.8560108/1‑94 (MQ=60)

                                                                       ATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTT                        >  SRR4302540.3679195/1‑94 (MQ=60)

                                                                       ATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTT                        >  SRR4302540.9602175/1‑94 (MQ=60)

                                                                       ATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTT                        >  SRR4302540.1793500/1‑94 (MQ=60)

                                                                       ATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTT                        >  SRR4302540.2042030/1‑94 (MQ=60)

                                                                        TATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTT                       <  SRR4302540.7927871/94‑1 (MQ=60)

                                                                        TATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTT                       <  SRR4302540.7927872/94‑1 (MQ=60)

                                                                        TATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTT                       >  SRR4302540.6830519/1‑94 (MQ=60)

                                                                         ACCATGCACAATACATAACCCCAGCTGGTCGCCATGAATTTCAATCCGGCCGGCATAGGTCCGCCCATGACGAGTCGAACCATCCGCCAGTTCA                      <  SRR4302540.861721/94‑1 (MQ=44)

                                                                          TCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCAgcggacgagtagaaatttccgcgagtgtat                     >  SRR4302540.1291868/1‑64 (MQ=60)

                                                                          TCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTAT                     <  SRR4302540.3030919/94‑1 (MQ=60)

                                                                          TCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTAT                     >  SRR4302540.1291869/1‑94 (MQ=60)

                                                                           CATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATG                    >  SRR4302540.726146/1‑94 (MQ=60)

                                                                           CATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATG                    <  SRR4302540.2836682/94‑1 (MQ=60)

                                                                            ATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGA                   <  SRR4302540.1017717/94‑1 (MQ=60)

                                                                             AGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAA                  <  SRR4302540.8209711/94‑1 (MQ=60)

                                                                               CACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACA                <  SRR4302540.9899447/94‑1 (MQ=60)

                                                                                ACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAA               <  SRR4302540.6106094/94‑1 (MQ=60)

                                                                                ACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAA               <  SRR4302540.7930823/94‑1 (MQ=60)

                                                                                ACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAA               >  SRR4302540.6672290/1‑94 (MQ=60)

                                                                                 CAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAA              >  SRR4302540.1397852/1‑94 (MQ=60)

                                                                                 CAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAA              <  SRR4302540.9816791/94‑1 (MQ=60)

                                                                                  AATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAA             <  SRR4302540.579994/94‑1 (MQ=60)

                                                                                  AATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAA             <  SRR4302540.579993/94‑1 (MQ=60)

                                                                                  AATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAA             <  SRR4302540.5138504/94‑1 (MQ=60)

                                                                                  AATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAA             <  SRR4302540.1269607/94‑1 (MQ=60)

                                                                                   ATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAAT            <  SRR4302540.3308542/94‑1 (MQ=60)

                                                                                   ATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAAT            <  SRR4302540.5100189/94‑1 (MQ=60)

                                                                                   ATAAATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAAT            <  SRR4302540.3308541/94‑1 (MQ=60)

                                                                                    TACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATT           >  SRR4302540.6710748/1‑94 (MQ=60)

                                                                                     ACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTC          >  SRR4302540.1438663/1‑94 (MQ=60)

                                                                                     ACAGAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTC          <  SRR4302540.2656225/94‑1 (MQ=60)

                                                                                      CATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCA         <  SRR4302540.6639938/94‑1 (MQ=60)

                                                                                      CATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCA         >  SRR4302540.912741/1‑94 (MQ=60)

                                                                                       ATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAA        >  SRR4302540.7823737/1‑94 (MQ=60)

                                                                                       ATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAA        >  SRR4302540.8188341/1‑94 (MQ=60)

                                                                                       ATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAA        >  SRR4302540.7659599/1‑94 (MQ=60)

                                                                                       ATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAA        <  SRR4302540.4017993/94‑1 (MQ=60)

                                                                                       ATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAA        >  SRR4302540.3859910/1‑94 (MQ=60)

                                                                                        TAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAA       >  SRR4302540.6710668/1‑94 (MQ=60)

                                                                                         AACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAG      <  SRR4302540.6783934/94‑1 (MQ=60)

                                                                                         AACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAG      >  SRR4302540.9136774/1‑94 (MQ=60)

                                                                                          ACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG     <  SRR4302540.5540919/94‑1 (MQ=60)

                                                                                          ACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG     <  SRR4302540.5540917/94‑1 (MQ=60)

                                                                                          ACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGCCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG     >  SRR4302540.4607531/1‑94 (MQ=60)

                                                                                           CACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGGT    <  SRR4302540.420777/94‑1 (MQ=60)

                                                                                             CGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGGTTT  >  SRR4302540.4604770/1‑94 (MQ=60)

                                                                                             CGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGGTTT  >  SRR4302540.607562/1‑94 (MQ=60)

TCAGTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACATGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGGTTT  >  NC_000913/1405033‑1405219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`