Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,477,747	A→G	I196V (ATT→GTT)	b1410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,477,747	0	A	G	91.4%	611.7 / 39.6	233	I196V (ATT→GTT)	b1410	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (11/9); new base G (126/87); total (137/96)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 238 total aligned reads displayed.

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

 ttattaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTg                                                                                        >  1:1368872/1‑93 (MQ=255)

  tattaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGc                                                                                       <  2:2134826/93‑1 (MQ=255)

   attaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGcc                                                                                      <  1:6569581/93‑1 (MQ=255)

     taATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGt                                                                                    >  2:4592837/1‑93 (MQ=255)

        ttCCGATGTGAAAGGCTTATAGTTGACCCAGTATCGGCAGCGGGGGGGGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTaa                                                                                 <  2:6421594/93‑1 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               <  2:9040437/93‑1 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               >  1:4611388/1‑93 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               >  2:5758917/1‑93 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               <  1:6582566/93‑1 (MQ=255)

           cTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAatat                                                                              <  1:8516030/93‑1 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  2:9446632/1‑93 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             <  1:6326495/93‑1 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  2:198265/1‑93 (MQ=255)

              tgtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCtt                                                                           >  2:4833591/1‑93 (MQ=255)

               gtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTg                                                                          >  1:784879/1‑93 (MQ=255)

                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         <  2:4385811/93‑1 (MQ=255)

                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         >  2:6966874/1‑93 (MQ=255)

                  aaaGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTc                                                                       <  2:592959/93‑1 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     >  1:8857547/1‑93 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     <  1:5030260/93‑1 (MQ=255)

                     ggACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    <  1:6464655/93‑1 (MQ=255)

                     ggACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    >  1:5124802/1‑93 (MQ=255)

                     ggACGCTATTTGACCCACAAGCGGCACCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    <  1:5338936/93‑1 (MQ=255)

                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGGGAATATCTTGCTCGAtc                                                                   >  1:8970705/1‑93 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 >  1:7908920/1‑93 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 <  2:7823803/93‑1 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                >  1:911141/1‑93 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                <  1:9580801/93‑1 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATctct                                                                >  1:1729805/1‑93 (MQ=255)

                          cTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTa                                                               >  1:9391049/1‑93 (MQ=255)

                           tATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAc                                                              >  1:7116285/1‑93 (MQ=255)

                           tATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAc                                                              >  1:2154851/1‑93 (MQ=255)

                            aTTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACa                                                             >  1:8871425/1‑93 (MQ=255)

                               tGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                          <  1:9574062/93‑1 (MQ=255)

                               tGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                          <  1:1611528/93‑1 (MQ=255)

                                gACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAc                                                         >  2:8609623/1‑93 (MQ=255)

                                  cccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGt                                                       >  1:5317952/1‑93 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTCAAAAACGTc                                                      >  1:2031957/1‑93 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                      <  2:6461367/93‑1 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                      <  2:2022229/93‑1 (MQ=255)

                                    cACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCt                                                     <  1:4206296/93‑1 (MQ=255)

                                      cGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGa                                                   <  2:1260651/93‑1 (MQ=255)

                                      cGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGa                                                   <  1:9765144/93‑1 (MQ=255)

                                        aTCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTCACCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAc                                                 >  1:849322/1‑93 (MQ=255)

                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                >  2:3489485/1‑93 (MQ=255)

                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                >  2:5224123/1‑93 (MQ=255)

                                           ggCAACGGGGGGCGAGTTTCAATAATTATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGta                                              <  1:1519927/93‑1 (MQ=255)

                                           ggCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGta                                              >  2:247563/1‑93 (MQ=255)

                                             cAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtatt                                            <  2:7451984/93‑1 (MQ=255)

                                               aCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtattat                                          <  2:1045695/93‑1 (MQ=255)

                                                cGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTattatt                                         <  1:9946797/93‑1 (MQ=255)

                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  1:2416865/93‑1 (MQ=255)

                                                   ggggCGAGTTTCAATAATGATCCGCTGATCCCACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTaga                                      <  1:3146602/93‑1 (MQ=255)

                                                   ggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTaga                                      >  1:6175419/1‑93 (MQ=255)

                                                    gggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTagag                                     <  1:2192356/93‑1 (MQ=255)

                                                    gggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTagag                                     <  1:804650/93‑1 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    >  2:3039717/1‑93 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    <  2:3258249/93‑1 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    >  1:1993536/1‑93 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    <  1:3959433/93‑1 (MQ=255)

                                                       cGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCAACAAAACGTCTGAACGTATTATTAGGGATg                                  <  2:7684076/93‑1 (MQ=255)

                                                        gAGTTTCAATAATGAACCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 <  2:7007995/93‑1 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggg                              >  1:2243468/1‑92 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              >  2:6118692/1‑93 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              >  1:8233258/1‑93 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              <  2:976729/93‑1 (MQ=255)

                                                            ttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcg                             <  2:9856857/93‑1 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  2:6561284/1‑93 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  1:8762862/1‑93 (MQ=255)

                                                                  aatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAtt                       >  2:3976889/1‑93 (MQ=255)

                                                                  aatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAtt                       >  2:7932655/1‑93 (MQ=255)

                                                                   atGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTa                      <  2:287445/93‑1 (MQ=255)

                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     >  1:9333487/1‑93 (MQ=255)

                                                                     gATCCGCTGATCAGACGGGCGGTTTCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    <  1:9275665/93‑1 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTagc                    >  1:7287303/1‑91 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    <  1:2759676/93‑1 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    <  2:6092571/93‑1 (MQ=255)

                                                                       tCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCt                  <  1:8815120/93‑1 (MQ=255)

                                                                       tCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCt                  <  1:7394509/93‑1 (MQ=255)

                                                                        ccGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTc                 >  1:8302616/1‑93 (MQ=255)

                                                                         cGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTCTTATTAGAGATGCGGCGGCGATTACGCTcc                >  1:3653961/1‑93 (MQ=255)

                                                                          gCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc               >  1:3214662/1‑93 (MQ=255)

                                                                          gCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc               >  2:5084056/1‑93 (MQ=255)

                                                                          gCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc               >  2:1743755/1‑93 (MQ=255)

                                                                           cTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTcccc              <  1:6079136/93‑1 (MQ=255)

                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccccc             >  2:7888568/1‑92 (MQ=255)

                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCa             >  1:5411646/1‑93 (MQ=255)

                                                                             gATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGGACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCAc            <  2:8783144/93‑1 (MQ=255)

                                                                             gATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCAc            >  1:2649211/1‑93 (MQ=255)

                                                                              aTCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACg           >  2:2656732/1‑93 (MQ=255)

                                                                              aTCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACg           >  2:2411095/1‑93 (MQ=255)

                                                                                 acacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCaa        >  2:2925178/1‑93 (MQ=255)

                                                                                  cacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAc       >  2:5464930/1‑93 (MQ=255)

                                                                                  cacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAc       >  1:1796795/1‑93 (MQ=255)

                                                                                  cacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAc       >  2:9152940/1‑93 (MQ=255)

                                                                                   acGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACt      >  1:5859718/1‑93 (MQ=255)

                                                                                    cGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt     >  2:389768/1‑93 (MQ=255)

                                                                                    cGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt     >  1:1082053/1‑93 (MQ=255)

                                                                                    cGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt     >  2:426064/1‑93 (MQ=255)

                                                                                       gCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTg  <  2:8433269/93‑1 (MQ=255)

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATC  >  NC_000913/1477654‑1477841

TTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGG                                                                                                >  SRR4302540.1364578/1‑94 (MQ=60)

 TTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGT                                                                                               <  SRR4302540.2059080/94‑1 (MQ=60)

 TTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGT                                                                                               >  SRR4302540.6358580/1‑94 (MQ=60)

 TTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAANGNNCCGCTGATCACACGGGCGGT                                                                                               >  SRR4302540.1974138/1‑94 (MQ=60)

  TTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTT                                                                                              >  SRR4302540.1586396/1‑94 (MQ=60)

   TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTG                                                                                             >  SRR4302540.9827297/1‑94 (MQ=60)

   TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTG                                                                                             <  SRR4302540.1256967/94‑1 (MQ=60)

   TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTG                                                                                             >  SRR4302540.1383370/1‑94 (MQ=60)

    TTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGC                                                                                            >  SRR4302540.5922884/1‑94 (MQ=60)

    TTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGC                                                                                            >  SRR4302540.9290969/1‑94 (MQ=60)

     TATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCC                                                                                           >  SRR4302540.2254628/1‑94 (MQ=60)

      ATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCG                                                                                          <  SRR4302540.4645763/94‑1 (MQ=60)

      ATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCG                                                                                          <  SRR4302540.6642376/94‑1 (MQ=60)

        TAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTT                                                                                        <  SRR4302540.8880872/94‑1 (MQ=60)

          ATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAA                                                                                      <  SRR4302540.163590/94‑1 (MQ=60)

           TTCCTATGTGAAAGGACGCTATTTGGCCCACGATCGGTAACGGGGGTCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAAT                                                                                     <  SRR4302540.9634010/94‑1 (MQ=60)

           TTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAAT                                                                                     >  SRR4302540.6838038/1‑94 (MQ=60)

           TTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAAT                                                                                     >  SRR4302540.2026301/1‑94 (MQ=60)

            TCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATA                                                                                    >  SRR4302540.4660880/1‑94 (MQ=60)

             CCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATAT                                                                                   <  SRR4302540.6655480/94‑1 (MQ=60)

             CCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATAT                                                                                   <  SRR4302540.7231468/94‑1 (MQ=60)

             CCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATAT                                                                                   <  SRR4302540.3402714/94‑1 (MQ=60)

              CTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATC                                                                                  >  SRR4302540.1006089/1‑94 (MQ=60)

              CTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATC                                                                                  <  SRR4302540.8614998/94‑1 (MQ=60)

              CTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATC                                                                                  <  SRR4302540.8614999/94‑1 (MQ=60)

               TATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCT                                                                                 <  SRR4302540.6396949/94‑1 (MQ=60)

                ATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTT                                                                                >  SRR4302540.6302246/1‑94 (MQ=60)

                 TGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTG                                                                               >  SRR4302540.793224/1‑94 (MQ=60)

                   TGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCT                                                                             >  SRR4302540.3220731/1‑94 (MQ=60)

                    GAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTC                                                                            >  SRR4302540.3010411/1‑94 (MQ=60)

                     AAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCGGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCG                                                                           >  SRR4302540.5311071/1‑94 (MQ=60)

                     AAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCG                                                                           >  SRR4302540.8904714/1‑94 (MQ=60)

                      AAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGA                                                                          >  SRR4302540.8960036/1‑94 (MQ=60)

                      AAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGA                                                                          >  SRR4302540.8960037/1‑94 (MQ=60)

                      AAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGA                                                                          >  SRR4302540.1752774/1‑94 (MQ=60)

                       AGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAT                                                                         <  SRR4302540.5084694/94‑1 (MQ=60)

                       AGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAT                                                                         >  SRR4302540.5180308/1‑94 (MQ=60)

                        GGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATC                                                                        >  SRR4302540.543867/1‑94 (MQ=60)

                        GGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATC                                                                        <  SRR4302540.6536598/94‑1 (MQ=60)

                        GGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGGGAATATCTTGCTCGATC                                                                        >  SRR4302540.9077204/1‑94 (MQ=60)

                        GGACGCTATTTGACCCACAAGCGGCACCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATC                                                                        <  SRR4302540.5397469/94‑1 (MQ=60)

                         GACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCT                                                                       >  SRR4302540.7582595/1‑94 (MQ=60)

                         GACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCT                                                                       >  SRR4302540.9442819/1‑94 (MQ=60)

                          ACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTC                                                                      >  SRR4302540.8001141/1‑94 (MQ=60)

                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                     >  SRR4302540.6529635/1‑94 (MQ=60)

                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                     >  SRR4302540.920785/1‑94 (MQ=60)

                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                     >  SRR4302540.2721523/1‑94 (MQ=60)

                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCT                                                                     >  SRR4302540.1748479/1‑94 (MQ=60)

                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCT                                                                     >  SRR4302540.3974420/1‑94 (MQ=60)

                            GCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTA                                                                    >  SRR4302540.9502802/1‑94 (MQ=60)

                            GCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTA                                                                    <  SRR4302540.9693934/94‑1 (MQ=60)

                            GCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGACGGTTGCCGTTAATATCTTGCTCGATCTCTA                                                                    >  SRR4302540.3497295/1‑94 (MQ=60)

                             CTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAC                                                                   <  SRR4302540.4213053/94‑1 (MQ=60)

                             CTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAC                                                                   >  SRR4302540.7195505/1‑94 (MQ=60)

                             CTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAC                                                                   >  SRR4302540.2178040/1‑94 (MQ=60)

                              TATTTGACCCACGATTGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACA                                                                  >  SRR4302540.8974090/1‑94 (MQ=60)

                              TATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACA                                                                  >  SRR4302540.8974091/1‑94 (MQ=60)

                              TATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACA                                                                  >  SRR4302540.9945679/1‑94 (MQ=60)

                              TATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACA                                                                  >  SRR4302540.4926748/1‑94 (MQ=60)

                                  TGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAC                                                              <  SRR4302540.9687151/94‑1 (MQ=60)

                                  TGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAC                                                              <  SRR4302540.1629179/94‑1 (MQ=60)

                                    ACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGT                                                            >  SRR4302540.5376182/1‑94 (MQ=60)

                                    ACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGT                                                            >  SRR4302540.5376183/1‑94 (MQ=60)

                                    ACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGC                                                            >  SRR4302540.8926516/1‑94 (MQ=60)

                                    ACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATAATCGGCGGATCAACCGGGGTGTGGCCGTTAATATCTTGGTCGATCTCTACACAAGGT                                                            >  SRR4302540.2898459/1‑94 (MQ=30)

                                     CCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTCAAAAACGTC                                                           >  SRR4302540.2053882/1‑94 (MQ=60)

                                      CCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCT                                                          >  SRR4302540.2063935/1‑94 (MQ=60)

                                       CACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTG                                                         >  SRR4302540.9468592/1‑94 (MQ=60)

                                       CACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTG                                                         <  SRR4302540.4252080/94‑1 (MQ=60)

                                        ACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGA                                                        <  SRR4302540.8003564/94‑1 (MQ=60)

                                         CGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAA                                                       <  SRR4302540.9879664/94‑1 (MQ=60)

                                          GATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTCACCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAC                                                      >  SRR4302540.858330/1‑94 (MQ=60)

                                           ATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACG                                                     >  SRR4302540.2800862/1‑94 (MQ=60)

                                              GGCAACGGGGGGCGAGTTTCAATAATTATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAT                                                  <  SRR4302540.1536749/94‑1 (MQ=60)

                                              CGCAACGGGCGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAT                                                  <  SRR4302540.1623250/94‑1 (MQ=60)

                                                CAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTA                                                <  SRR4302540.9689040/94‑1 (MQ=60)

                                                 AACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTAT                                               >  SRR4302540.2150917/1‑94 (MQ=60)

                                                  ACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATT                                              >  SRR4302540.7941748/1‑94 (MQ=60)

                                                  ACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATT                                              <  SRR4302540.8724663/94‑1 (MQ=60)

                                                   CGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTA                                             >  SRR4302540.6839078/1‑94 (MQ=60)

                                                   CGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTA                                             <  SRR4302540.10062937/94‑1 (MQ=60)

                                                    GGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAG                                            <  SRR4302540.2442909/94‑1 (MQ=60)

                                                     GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGA                                           >  SRR4302540.6244217/1‑94 (MQ=60)

                                                      GGGGCGAGTTTCAATAATGATCCGCTGATCCCACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAG                                          <  SRR4302540.3180880/94‑1 (MQ=60)

                                                      GGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAG                                          >  SRR4302540.6234252/1‑94 (MQ=60)

                                                      GGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAG                                          >  SRR4302540.6234253/1‑94 (MQ=60)

                                                      GGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAG                                          >  SRR4302540.6553504/1‑94 (MQ=60)

                                                       GGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGA                                         >  SRR4302540.2015139/1‑94 (MQ=60)

                                                       GGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGA                                         <  SRR4302540.813196/94‑1 (MQ=60)

                                                       GGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGA                                         <  SRR4302540.813195/94‑1 (MQ=60)

                                                       GGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGA                                         <  SRR4302540.2216034/94‑1 (MQ=60)

                                                       GGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGA                                         <  SRR4302540.9490814/94‑1 (MQ=60)

                                                        GGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGAT                                        <  SRR4302540.4002143/94‑1 (MQ=60)

                                                        GACGAGTATCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCACGATCTCTACAAAACGTCTGAACGTATTATTAGAGAT                                        <  SRR4302540.9084969/94‑1 (MQ=60)

                                                         GCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATG                                       <  SRR4302540.2368980/94‑1 (MQ=60)

                                                           GAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCG                                     <  SRR4302540.2412764/94‑1 (MQ=60)

                                                             GTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGG                                   >  SRR4302540.2267582/1‑94 (MQ=60)

                                                             GTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGC                                   >  SRR4302540.8328961/1‑94 (MQ=60)

                                                                   ATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAT                             <  SRR4302540.5194739/94‑1 (MQ=60)

                                                                   ATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAT                             >  SRR4302540.8864458/1‑94 (MQ=60)

                                                                    TAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATT                            >  SRR4302540.9704779/1‑94 (MQ=60)

                                                                    TAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATT                            <  SRR4302540.6646482/94‑1 (MQ=60)

                                                                     AATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTA                           >  SRR4302540.837219/1‑94 (MQ=60)

                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                          <  SRR4302540.9332988/94‑1 (MQ=60)

                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                          <  SRR4302540.8126226/94‑1 (MQ=60)

                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                          <  SRR4302540.7418947/94‑1 (MQ=60)

                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                          <  SRR4302540.850802/94‑1 (MQ=60)

                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                          >  SRR4302540.9444767/1‑94 (MQ=60)

                                                                       TGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAgc                         >  SRR4302540.7368623/1‑92 (MQ=60)

                                                                       TGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACG                         >  SRR4302540.6467093/1‑94 (MQ=60)

                                                                        GATCCGCTGATCAGACGGGCGGTTTCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGC                        <  SRR4302540.9386563/94‑1 (MQ=60)

                                                                        GATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGC                        <  SRR4302540.2789494/94‑1 (MQ=60)

                                                                         ATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCT                       <  SRR4302540.2676057/94‑1 (MQ=60)

                                                                         ATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCT                       >  SRR4302540.4647507/1‑94 (MQ=60)

                                                                          TCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTC                      >  SRR4302540.8399020/1‑94 (MQ=60)

                                                                          TCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTC                      <  SRR4302540.8917038/94‑1 (MQ=60)

                                                                          TCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTC                      <  SRR4302540.7476902/94‑1 (MQ=60)

                                                                           CCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTCTTATTAGAGATGCGGCGGCGATTACGCTCC                     >  SRR4302540.3693981/1‑94 (MQ=60)

                                                                           CCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCC                     <  SRR4302540.1930366/94‑1 (MQ=60)

                                                                            CGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCC                    >  SRR4302540.3249571/1‑94 (MQ=60)

                                                                            CGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCC                    <  SRR4302540.1888599/94‑1 (MQ=60)

                                                                              CTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCC                  >  SRR4302540.7651695/1‑94 (MQ=60)

                                                                              CTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCC                  >  SRR4302540.5471147/1‑94 (MQ=60)

                                                                              CTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCA                  <  SRR4302540.6146734/94‑1 (MQ=60)

                                                                              CTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCA                  <  SRR4302540.6146733/94‑1 (MQ=60)

                                                                              CTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCA                  >  SRR4302540.5471148/1‑94 (MQ=60)

                                                                               TGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCAC                 >  SRR4302540.2677952/1‑94 (MQ=60)

                                                                                    ACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAC            >  SRR4302540.1816340/1‑94 (MQ=60)

                                                                                     CACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACT           <  SRR4302540.2200781/94‑1 (MQ=60)

                                                                                     CACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACT           >  SRR4302540.5924519/1‑94 (MQ=60)

                                                                                      ACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTT          <  SRR4302540.5160646/94‑1 (MQ=60)

                                                                                      ACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTT          >  SRR4302540.1093134/1‑94 (MQ=60)

                                                                                       CGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACTTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTC         <  SRR4302540.8413998/94‑1 (MQ=60)

                                                                                        GGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTGCT        >  SRR4302540.1488306/1‑94 (MQ=60)

                                                                                           CGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGATCGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGAT     <  SRR4302540.6177048/94‑1 (MQ=60)

                                                                                           CGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGAT     >  SRR4302540.10036934/1‑94 (MQ=60)

                                                                                            GGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATA    >  SRR4302540.5324340/1‑94 (MQ=60)

                                                                                            GGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATA    >  SRR4302540.3014828/1‑94 (MQ=60)

                                                                                            GGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATA    >  SRR4302540.7566750/1‑94 (MQ=60)

                                                                                             GTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT   <  SRR4302540.4320317/94‑1 (MQ=60)

                                                                                             GTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT   >  SRR4302540.786462/1‑94 (MQ=60)

                                                                                             GTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTG‑ACGTATTATTAGAGATGCGGCAGCGATTACGCTCCCCACGCAACTTCTGATATC  <  SRR4302540.1881330/94‑1 (MQ=60)

TTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATC  >  NC_000913/1477654‑1477841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`