Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,477,747 A→G I196V (ATT→GTT)  b1410 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,477,7470AG99.4% 575.0 / NA 179I196V (ATT→GTT) b1410hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (94/84);  minor base T (0/1);  total (94/85)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836
                                                                                          |                                                                                         
tttATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGtt                                                                                         >  1:3167422/1‑93 (MQ=255)
    ttaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCg                                                                                     >  2:1309502/1‑93 (MQ=255)
     taATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGt                                                                                    >  2:4246729/1‑93 (MQ=255)
       aTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTa                                                                                  >  2:176262/1‑93 (MQ=255)
       aTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTa                                                                                  <  2:1400132/93‑1 (MQ=255)
         tCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAat                                                                                >  2:6060660/1‑93 (MQ=255)
          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               <  2:1993336/93‑1 (MQ=255)
           cTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAatat                                                                              >  1:4075486/1‑93 (MQ=255)
            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  1:2417901/1‑93 (MQ=255)
            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  2:4257343/1‑93 (MQ=255)
             aTGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCt                                                                            <  2:3775240/93‑1 (MQ=255)
              tgtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCtt                                                                           >  1:1759536/1‑93 (MQ=255)
              tgtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCtt                                                                           <  1:1069182/93‑1 (MQ=255)
               gtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTg                                                                          <  2:6044379/93‑1 (MQ=255)
                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         <  2:4114426/93‑1 (MQ=255)
                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         <  2:5970377/93‑1 (MQ=255)
                 gAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCt                                                                        >  1:1234488/1‑93 (MQ=255)
                 gAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCt                                                                        >  2:1540176/1‑93 (MQ=255)
                  aaaGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTc                                                                       >  1:2206141/1‑93 (MQ=255)
                   aaGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCg                                                                      >  1:6294839/1‑93 (MQ=255)
                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     >  1:1767257/1‑93 (MQ=255)
                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     >  2:317560/1‑93 (MQ=255)
                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGCGGTTTGCGGTT‑ATATCTTTCttggt                                                                    >  1:1267090/1‑89 (MQ=255)
                     ggACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    <  2:754681/93‑1 (MQ=255)
                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtc                                                                   <  2:2279122/93‑1 (MQ=255)
                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtc                                                                   >  1:3325839/1‑93 (MQ=255)
                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtc                                                                   <  1:5168728/93‑1 (MQ=255)
                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 >  2:2841162/1‑93 (MQ=255)
                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 <  2:4538363/93‑1 (MQ=255)
                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 >  2:5633345/1‑93 (MQ=255)
                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 <  1:2681663/93‑1 (MQ=255)
                        cGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                >  1:3719100/1‑93 (MQ=255)
                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                >  1:3585618/1‑93 (MQ=255)
                           tATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAc                                                              >  1:4287747/1‑93 (MQ=255)
                             tttGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaa                                                            >  2:3922449/1‑93 (MQ=255)
                              ttGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaa                                                           >  1:3359706/1‑93 (MQ=255)
                              ttGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaa                                                           >  1:2276732/1‑93 (MQ=255)
                               tGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                          <  1:2130633/93‑1 (MQ=255)
                                  cccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGt                                                       <  1:4530159/93‑1 (MQ=255)
                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                      >  2:6224029/1‑93 (MQ=255)
                                    cACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCt                                                     <  1:845257/93‑1 (MQ=255)
                                     aCGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTg                                                    >  1:3178303/1‑93 (MQ=255)
                                      cGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTAGATCTCTACAAAACGTCTGa                                                   <  2:1069882/93‑1 (MQ=255)
                                       gATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                  <  1:421447/93‑1 (MQ=255)
                                       gATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                  <  1:1786223/93‑1 (MQ=255)
                                        aTCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAc                                                 >  1:5731266/1‑93 (MQ=255)
                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                <  1:3726710/93‑1 (MQ=255)
                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                >  2:2863249/1‑93 (MQ=255)
                                          cGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGt                                               <  1:1363880/93‑1 (MQ=255)
                                           ggCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGta                                              >  2:2074081/1‑93 (MQ=255)
                                            gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                             >  1:4884901/1‑93 (MQ=255)
                                            gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                             <  2:193914/93‑1 (MQ=255)
                                            gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                             <  2:2145557/93‑1 (MQ=255)
                                             cAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtatt                                            <  2:3385461/93‑1 (MQ=255)
                                              aaCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtatta                                           >  2:5159713/1‑93 (MQ=255)
                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  2:4179078/93‑1 (MQ=255)
                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  2:1841282/93‑1 (MQ=255)
                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        >  2:2855062/1‑93 (MQ=255)
                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  2:858721/93‑1 (MQ=255)
                                                  gggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTag                                       <  1:4612535/93‑1 (MQ=255)
                                                    gggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTagag                                     >  2:3045448/1‑93 (MQ=255)
                                                      gCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGAt                                   >  2:4867546/1‑93 (MQ=255)
                                                        gAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 <  2:2684365/93‑1 (MQ=255)
                                                        gAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 >  2:3508752/1‑93 (MQ=255)
                                                        gAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 >  2:521330/1‑93 (MQ=255)
                                                        gAGTTTCAATAATGACCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 <  2:6001052/93‑1 (MQ=255)
                                                         aGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcg                                <  2:4987282/93‑1 (MQ=255)
                                                          gTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcgg                               >  2:5264952/1‑93 (MQ=255)
                                                          gTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcgg                               <  1:5593062/93‑1 (MQ=255)
                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              >  2:5537304/1‑93 (MQ=255)
                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAAGTCTGAACGAATTATTAGAGATgcggc                              >  2:2035933/1‑93 (MQ=255)
                                                            ttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcg                             <  1:3105851/93‑1 (MQ=255)
                                                             tCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcgg                            >  2:3257679/1‑93 (MQ=255)
                                                              cAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTTCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggc                           <  2:63023/93‑1 (MQ=255)
                                                               aataatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggcg                          >  1:110072/1‑93 (MQ=255)
                                                                ataatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGa                         >  1:3999763/1‑93 (MQ=255)
                                                                ataatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGa                         >  2:649289/1‑93 (MQ=255)
                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        <  1:189709/93‑1 (MQ=255)
                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  2:242689/1‑93 (MQ=255)
                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  2:2221104/1‑93 (MQ=255)
                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        <  2:3441199/93‑1 (MQ=255)
                                                                   atGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTa                      >  1:6101835/1‑93 (MQ=255)
                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     <  1:349385/93‑1 (MQ=255)
                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     <  2:4749267/93‑1 (MQ=255)
                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     >  1:2903508/1‑93 (MQ=255)
                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     >  1:4492068/1‑93 (MQ=255)
                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    >  1:592821/1‑93 (MQ=255)
                                                                      aTCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGc                   <  2:5449491/93‑1 (MQ=255)
                                                                        ccGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTc                 >  1:3433097/1‑93 (MQ=255)
                                                                           cTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTcccc              <  2:5576819/93‑1 (MQ=255)
                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCa             >  2:4972478/1‑93 (MQ=255)
                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCa             <  1:1475509/93‑1 (MQ=255)
                                                                               tCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGc          >  1:2106291/1‑93 (MQ=255)
                                                                                cacaCGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCa         >  1:5482770/1‑93 (MQ=255)
                                                                                     gggCGTTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc    <  2:2765440/93‑1 (MQ=255)
                                                                                     gggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc    <  2:4796637/93‑1 (MQ=255)
                                                                                     gggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATGACGCTCCCCACGCAACTTc    <  2:6137469/93‑1 (MQ=255)
                                                                                      ggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCt   <  1:900949/93‑1 (MQ=255)
                                                                                      ggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCt   >  2:1637769/1‑93 (MQ=255)
                                                                                       gCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTg  >  2:2933777/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT  >  NC_000913/1477654‑1477840
                                                                                             |                                                                                             
TTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGG                                                                                               >  SRR4302533.2382231/1‑94 (MQ=60)
  TTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTT                                                                                             <  SRR4302533.4281290/94‑1 (MQ=60)
  TTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTT                                                                                             >  SRR4302533.3186912/1‑94 (MQ=60)
             CCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATAT                                                                                  >  SRR4302533.4100295/1‑94 (MQ=60)
             CCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATAT                                                                                  >  SRR4302533.4046441/1‑94 (MQ=60)
              CTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATC                                                                                 >  SRR4302533.2432834/1‑94 (MQ=60)
               TATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCT                                                                                <  SRR4302533.3713150/94‑1 (MQ=60)
                ATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTT                                                                               >  SRR4302533.1770261/1‑94 (MQ=60)
                 TGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTG                                                                              <  SRR4302533.1075477/94‑1 (MQ=60)
                 TGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTG                                                                              <  SRR4302533.4669278/94‑1 (MQ=60)
                 TGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTG                                                                              <  SRR4302533.3384702/94‑1 (MQ=60)
                   TGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATCTCTTTCT                                                                            >  SRR4302533.223530/1‑94 (MQ=60)
                   TGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCT                                                                            >  SRR4302533.4387343/1‑94 (MQ=60)
                   TGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCT                                                                            >  SRR4302533.1241869/1‑94 (MQ=60)
                    GAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTC                                                                           >  SRR4302533.1430414/1‑94 (MQ=60)
                    GAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTC                                                                           >  SRR4302533.2219746/1‑94 (MQ=60)
                     AAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGTTGATCACACGGGCGGTTGCCGTTAAAATCTTGCTCG                                                                          <  SRR4302533.5495024/94‑1 (MQ=60)
                     AAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCG                                                                          >  SRR4302533.6332361/1‑94 (MQ=60)
                      AAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGA                                                                         >  SRR4302533.4308507/1‑94 (MQ=60)
                      AAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGA                                                                         >  SRR4302533.1778017/1‑94 (MQ=60)
                        GGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATC                                                                       >  SRR4302533.3346388/1‑94 (MQ=60)
                        AGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATC                                                                       <  SRR4302533.5933986/94‑1 (MQ=60)
                         GACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCT                                                                      <  SRR4302533.5199926/94‑1 (MQ=60)
                          ACGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                    >  SRR4302533.3741928/1‑94 (MQ=60)
                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                    >  SRR4302533.3607701/1‑94 (MQ=60)
                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                    <  SRR4302533.2698097/94‑1 (MQ=60)
                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                    >  SRR4302533.6174442/1‑94 (MQ=60)
                           CGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCT                                                                    >  SRR4302533.1223940/1‑94 (MQ=60)
                             CTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAC                                                                  >  SRR4302533.4313530/1‑94 (MQ=60)
                              TATTGGCCCCACGATCGGCAACGGTGGGCGAGTGACAATAATGATCCGCTGATCACACGGGCGGTTGCCGATAATATCATGCTCGATCTCTACA                                                                 <  SRR4302533.3042442/94‑1 (MQ=60)
                                TTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAA                                                               >  SRR4302533.2290832/1‑94 (MQ=60)
                                TTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAA                                                               <  SRR4302533.1502706/94‑1 (MQ=60)
                                TTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAA                                                               >  SRR4302533.3380463/1‑94 (MQ=60)
                                  TGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAC                                                             <  SRR4302533.2143769/94‑1 (MQ=60)
                                    ACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGT                                                           >  SRR4302533.1786295/1‑94 (MQ=60)
                                     CCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTC                                                          >  SRR4302533.2132321/1‑94 (MQ=60)
                                     CCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTC                                                          <  SRR4302533.4557592/94‑1 (MQ=60)
                                       CACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTG                                                        >  SRR4302533.3197853/1‑94 (MQ=60)
                                       CACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTG                                                        <  SRR4302533.850202/94‑1 (MQ=60)
                                       CACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTG                                                        >  SRR4302533.3111529/1‑94 (MQ=60)
                                        ACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGA                                                       <  SRR4302533.5563866/94‑1 (MQ=60)
                                          GATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAC                                                     <  SRR4302533.1797083/94‑1 (MQ=60)
                                          GATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAC                                                     <  SRR4302533.423967/94‑1 (MQ=60)
                                          GATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAC                                                     >  SRR4302533.2317956/1‑94 (MQ=60)
                                          GATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAC                                                     <  SRR4302533.3478275/94‑1 (MQ=60)
                                          GATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAC                                                     >  SRR4302533.5765869/1‑94 (MQ=60)
                                            TCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGT                                                   <  SRR4302533.3749571/94‑1 (MQ=60)
                                             CGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTA                                                  <  SRR4302533.1372073/94‑1 (MQ=60)
                                              GGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAT                                                 >  SRR4302533.4914495/1‑94 (MQ=60)
                                              GGCAACGGGGGGCGAGCTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGGACGTAT                                                 >  SRR4302533.6013309/1‑94 (MQ=60)
                                                CAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTA                                               <  SRR4302533.4210595/94‑1 (MQ=60)
                                                    GGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTANTANCTTGCTCGNTCTCTANNAANCGTCTGAACGNANTNTNAG                                           >  SRR4302533.1764175/1‑94 (MQ=60)
                                                     GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGA                                          <  SRR4302533.894754/94‑1 (MQ=60)
                                                     GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGA                                          <  SRR4302533.4640479/94‑1 (MQ=60)
                                                      GGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAG                                         <  SRR4302533.3516089/94‑1 (MQ=60)
                                                      GGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAG                                         <  SRR4302533.2042202/94‑1 (MQ=60)
                                                       GGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGA                                        >  SRR4302533.1315073/1‑94 (MQ=60)
                                                         GCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATG                                      <  SRR4302533.215166/94‑1 (MQ=60)
                                                         GCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATG                                      <  SRR4302533.3140244/94‑1 (MQ=60)
                                                         GCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATG                                      >  SRR4302533.3902729/1‑94 (MQ=60)
                                                            AGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGG                                   >  SRR4302533.748103/1‑94 (MQ=60)
                                                             GTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGC                                  <  SRR4302533.5626851/94‑1 (MQ=60)
                                                               TTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGG                                <  SRR4302533.3124942/94‑1 (MQ=60)
                                                                 CAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCG                              >  SRR4302533.110770/1‑94 (MQ=60)
                                                                 CAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCG                              >  SRR4302533.1264535/1‑94 (MQ=60)
                                                                  AATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGA                             >  SRR4302533.4024115/1‑94 (MQ=60)
                                                                   ATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAT                            >  SRR4302533.5058570/1‑94 (MQ=60)
                                                                    TAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATT                           >  SRR4302533.4596824/1‑94 (MQ=60)
                                                                    TAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATT                           <  SRR4302533.190892/94‑1 (MQ=60)
                                                                     AATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTA                          >  SRR4302533.6138675/1‑94 (MQ=60)
                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                         >  SRR4302533.4519270/1‑94 (MQ=60)
                                                                      ATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAC                         >  SRR4302533.2921341/1‑94 (MQ=60)
                                                                       TGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACG                        <  SRR4302533.1830735/94‑1 (MQ=60)
                                                                       TGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACG                        >  SRR4302533.596321/1‑94 (MQ=60)
                                                                       TGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACG                        <  SRR4302533.351517/94‑1 (MQ=60)
                                                                        GATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGC                       >  SRR4302533.3376760/1‑94 (MQ=60)
                                                                        GATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGC                       >  SRR4302533.2567154/1‑94 (MQ=60)
                                                                          TCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTC                     >  SRR4302533.3454306/1‑94 (MQ=60)
                                                                              CTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCC                 >  SRR4302533.2856263/1‑94 (MQ=60)
                                                                               TGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCAC                <  SRR4302533.1484428/94‑1 (MQ=60)
                                                                                 ATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGC              >  SRR4302533.2119280/1‑94 (MQ=60)
                                                                                  TCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCA             >  SRR4302533.5515820/1‑94 (MQ=60)
                                                                                        GGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCT       >  SRR4302533.3214813/1‑94 (MQ=60)
                                                                                         GGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG      <  SRR4302533.906233/94‑1 (MQ=60)
                                                                                            GGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATA   >  SRR4302533.4169630/1‑94 (MQ=60)
                                                                                             GTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT  >  SRR4302533.4244138/1‑94 (MQ=60)
                                                                                             GTTGCAGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGGATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT  <  SRR4302533.3222251/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATAT  >  NC_000913/1477654‑1477840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑