Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,478,094 A→G G311G (GGA→GGG)  b1410 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,478,0940AG99.6% 805.9 / NA 261G311G (GGA→GGG) b1410hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (130/130);  total (130/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

TTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT  >  NC_000913/1478004‑1478183
                                                                                          |                                                                                         
   atatGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGt                                                                                      >  2:6237751/1‑93 (MQ=255)
   atatGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGt                                                                                      <  2:8675882/93‑1 (MQ=255)
        gtAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCAt                                                                                 <  2:8814446/92‑1 (MQ=255)
          aaCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTa                                                                               <  1:2364050/93‑1 (MQ=255)
          aaCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTa                                                                               <  2:6925319/93‑1 (MQ=255)
           aCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTAt                                                                              <  2:1778065/93‑1 (MQ=255)
            cAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATc                                                                             <  2:8173989/93‑1 (MQ=255)
              aaTCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGc                                                                           <  2:428192/93‑1 (MQ=255)
                tCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCg                                                                         <  2:2840894/93‑1 (MQ=255)
                tCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCg                                                                         <  1:3749331/93‑1 (MQ=255)
                tCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCg                                                                         >  1:7606139/1‑93 (MQ=255)
                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       >  2:9291904/1‑93 (MQ=255)
                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       <  2:7863032/93‑1 (MQ=255)
                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       <  1:6383747/93‑1 (MQ=255)
                   cAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGTCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGtt                                                                      <  1:5331651/93‑1 (MQ=255)
                   cAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGtt                                                                      <  1:1908230/93‑1 (MQ=255)
                     aaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGa                                                                    <  2:1855846/93‑1 (MQ=255)
                      aaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGAt                                                                   <  2:9467360/93‑1 (MQ=255)
                         tttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTa                                                                >  2:2262689/1‑93 (MQ=255)
                          ttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAg                                                               >  1:2144382/1‑93 (MQ=255)
                          ttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAg                                                               <  2:5411645/93‑1 (MQ=255)
                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             <  2:4003584/93‑1 (MQ=255)
                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             <  2:5099465/93‑1 (MQ=255)
                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             <  2:1729805/93‑1 (MQ=255)
                             aTTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGt                                                            <  2:3473111/93‑1 (MQ=255)
                              ttATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTa                                                           <  1:6724965/93‑1 (MQ=255)
                               tATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGACCGTGCCAGTATCGCCGGCTGATTTAGCGTAt                                                          >  1:5913864/1‑93 (MQ=255)
                               tATCAGCATGAAGACTGCACAGGAACATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTAt                                                          <  1:8980228/93‑1 (MQ=255)
                                aTCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATc                                                         <  2:361849/93‑1 (MQ=255)
                                 tCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCg                                                        <  2:2041934/93‑1 (MQ=255)
                                   aGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCt                                                      <  1:9832667/93‑1 (MQ=255)
                                   aGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCt                                                      >  2:6109645/1‑93 (MQ=255)
                                     cATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCttt                                                    <  2:6764324/93‑1 (MQ=255)
                                     cATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCttt                                                    <  2:8053203/93‑1 (MQ=255)
                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGGCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCcttt                                                   >  2:8329911/1‑92 (MQ=255)
                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTa                                                   >  2:1267429/1‑93 (MQ=255)
                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTa                                                   >  1:7917092/1‑93 (MQ=255)
                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTa                                                   <  2:1993536/93‑1 (MQ=255)
                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGGTTTAGCGTATCGCTTTAt                                                  >  2:2290129/1‑93 (MQ=255)
                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  >  1:610833/1‑93 (MQ=255)
                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  >  1:337666/1‑93 (MQ=255)
                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  <  1:8255615/93‑1 (MQ=255)
                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGCTGATTTAGCGTATCGCTTTAt                                                  >  1:10006018/1‑93 (MQ=255)
                                        gAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATg                                                 <  1:408009/93‑1 (MQ=255)
                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                >  2:4467354/1‑93 (MQ=255)
                                          aGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCg                                               <  1:2920935/93‑1 (MQ=255)
                                          aGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCg                                               <  1:6278130/93‑1 (MQ=255)
                                           gACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGa                                              >  1:8751098/1‑93 (MQ=255)
                                            aCTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGaa                                             <  1:3039717/93‑1 (MQ=255)
                                              tGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                           >  2:5727017/1‑93 (MQ=255)
                                              tGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                           >  2:7582161/1‑93 (MQ=255)
                                               gCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAgg                                          >  2:2906856/1‑93 (MQ=255)
                                                cacaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGc                                         <  2:6140391/93‑1 (MQ=255)
                                                cacaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGc                                         <  2:8531978/93‑1 (MQ=255)
                                                 acaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCg                                        >  2:7121049/1‑93 (MQ=255)
                                                 acaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATCATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCg                                        >  2:3335611/1‑93 (MQ=255)
                                                  caGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGCTGATTTAGCGTATCGCTTTATGCGAAAGGCGa                                       >  2:1234809/1‑93 (MQ=255)
                                                   aGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGAt                                      <  2:2517007/93‑1 (MQ=255)
                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  1:3235475/1‑93 (MQ=255)
                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     <  2:7921192/93‑1 (MQ=255)
                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  1:4712923/1‑93 (MQ=255)
                                                    ggACCATCAGCGGATCGATGGCGGATACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  2:7278580/1‑93 (MQ=255)
                                                     gACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGa                                    <  2:6395895/93‑1 (MQ=255)
                                                       ccATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGaaa                                  >  2:9551331/1‑93 (MQ=255)
                                                       ccATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGaaa                                  <  1:4031614/93‑1 (MQ=255)
                                                         aTCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAtt                                <  1:1373517/93‑1 (MQ=255)
                                                         aTCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAtt                                >  2:5699761/1‑93 (MQ=255)
                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               <  1:5617186/93‑1 (MQ=255)
                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               >  2:5382859/1‑93 (MQ=255)
                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               <  1:8677751/93‑1 (MQ=255)
                                                           cAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGt                              >  1:4504944/1‑93 (MQ=255)
                                                             gCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTc                            >  2:2086529/1‑93 (MQ=255)
                                                             gCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTc                            <  2:9051613/93‑1 (MQ=255)
                                                              cGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCTATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCg                           <  2:9261078/93‑1 (MQ=255)
                                                              cGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGGAATTGTTCg                           >  1:3951724/1‑93 (MQ=255)
                                                               ggATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCgg                          >  1:1419498/1‑93 (MQ=255)
                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         <  2:8293049/93‑1 (MQ=255)
                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         >  1:1666522/1‑93 (MQ=255)
                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        >  1:856565/1‑93 (MQ=255)
                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        <  1:2691956/93‑1 (MQ=255)
                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        <  1:3585047/93‑1 (MQ=255)
                                                                  tCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGAc                       <  2:2647359/93‑1 (MQ=255)
                                                                   cGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCgggcag                      >  1:1372706/1‑90 (MQ=255)
                                                                    gATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGc                     >  1:5394623/1‑93 (MQ=255)
                                                                      tGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCAc                   <  2:1413100/93‑1 (MQ=255)
                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATTAAATTGTTCGGGACGCACTCTTc              >  2:5540950/1‑93 (MQ=255)
                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTc              >  1:9452165/1‑93 (MQ=255)
                                                                            cgaCTTGCTGGTGGTCCCGTGCCATTATCGCCGCTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCg             <  2:1056107/91‑1 (MQ=255)
                                                                            cTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCg             >  1:1095230/1‑93 (MQ=255)
                                                                            cTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCg             <  1:704932/93‑1 (MQ=255)
                                                                             tACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCgg            <  1:4659978/93‑1 (MQ=255)
                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          <  1:5074272/93‑1 (MQ=255)
                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          >  1:2045483/1‑93 (MQ=255)
                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          <  2:5009566/93‑1 (MQ=255)
                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          <  2:4446313/93‑1 (MQ=255)
                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         <  1:8042550/93‑1 (MQ=255)
                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         >  2:4706753/1‑93 (MQ=255)
                                                                                   cTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGc      >  2:410777/1‑93 (MQ=255)
                                                                                      gtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCAtt   >  1:1286268/1‑92 (MQ=255)
                                                                                      gtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGAACTCTTCGGGCCTGCAtc   >  2:3585369/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT  >  NC_000913/1478004‑1478183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGG  >  NC_000913/1478001‑1478186
                                                                                             |                                                                                            
TCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGG                                                                                              <  SRR4302540.9541275/94‑1 (MQ=60)
TCGTTAATAGGCTAACAAACCGCAAAAATTTGATTA‑CCGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGC                                                                                             <  SRR4302540.9276253/94‑1 (MQ=60)
 CGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGC                                                                                             <  SRR4302540.549736/94‑1 (MQ=60)
 CGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGC                                                                                             >  SRR4302540.8027407/1‑94 (MQ=60)
 CGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGC                                                                                             >  SRR4302540.826706/1‑94 (MQ=60)
  GTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCC                                                                                            >  SRR4302540.6519304/1‑94 (MQ=60)
      ATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTG                                                                                        >  SRR4302540.3080636/1‑94 (MQ=60)
        ATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCC                                                                                      <  SRR4302540.5947642/94‑1 (MQ=60)
        ATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCC                                                                                      <  SRR4302540.6004862/94‑1 (MQ=60)
         TGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCA                                                                                     >  SRR4302540.985900/1‑94 (MQ=60)
           CTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATT                                                                                   >  SRR4302540.1010792/1‑94 (MQ=60)
             AACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTAT                                                                                 <  SRR4302540.2389508/94‑1 (MQ=60)
             AACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTAT                                                                                 >  SRR4302540.527715/1‑94 (MQ=60)
              ACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATC                                                                                <  SRR4302540.8850055/94‑1 (MQ=60)
                 AATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCC                                                                             <  SRR4302540.2437085/94‑1 (MQ=60)
                  ATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCG                                                                            >  SRR4302540.7691611/1‑94 (MQ=60)
                   TCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGG                                                                           <  SRR4302540.3790237/94‑1 (MQ=60)
                    CGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGT                                                                          >  SRR4302540.8219687/1‑94 (MQ=60)
                     GCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTT                                                                         <  SRR4302540.6454811/94‑1 (MQ=60)
                      CAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGTCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTG                                                                        <  SRR4302540.5390066/94‑1 (MQ=60)
                      CAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTG                                                                        <  SRR4302540.917387/94‑1 (MQ=60)
                      CAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTG                                                                        <  SRR4302540.1929080/94‑1 (MQ=60)
                      CAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTG                                                                        >  SRR4302540.5403110/1‑94 (MQ=60)
                         AAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATT                                                                     >  SRR4302540.1398988/1‑94 (MQ=60)
                         AAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATT                                                                     >  SRR4302540.8957930/1‑94 (MQ=60)
                         AAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTAGCACCGCGCCATTATCGCCGGctgcgt                                                                     >  SRR4302540.1398989/1‑88 (MQ=60)
                           ATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTA                                                                   <  SRR4302540.1485265/94‑1 (MQ=60)
                            TTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAG                                                                  >  SRR4302540.2167433/1‑94 (MQ=60)
                            TTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAG                                                                  >  SRR4302540.2386591/1‑94 (MQ=60)
                             TTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGC                                                                 >  SRR4302540.8357696/1‑94 (MQ=60)
                             TTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGC                                                                 >  SRR4302540.8357695/1‑94 (MQ=60)
                              TGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCG                                                                >  SRR4302540.2648427/1‑94 (MQ=60)
                                 TTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTAT                                                             <  SRR4302540.6799196/94‑1 (MQ=60)
                                 TTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTAT                                                             <  SRR4302540.8076064/94‑1 (MQ=60)
                                 TTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGACCGTGCCAGTATCGCCGGCTGATTTAGCGTAT                                                             >  SRR4302540.5979200/1‑94 (MQ=60)
                                  TATCAGCATGAAGACTGCACAGGATCATCAACGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATC                                                            <  SRR4302540.3533444/94‑1 (MQ=60)
                                  TATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATC                                                            >  SRR4302540.4521264/1‑94 (MQ=60)
                                  TATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATC                                                            <  SRR4302540.4762414/94‑1 (MQ=60)
                                  TATCAGCATGAAGACTGCACAGGAACATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATC                                                            <  SRR4302540.9087242/94‑1 (MQ=60)
                                   ATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCG                                                           <  SRR4302540.6961855/94‑1 (MQ=60)
                                    TCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGC                                                          >  SRR4302540.3331016/1‑94 (MQ=60)
                                     CAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCT                                                         <  SRR4302540.7645845/94‑1 (MQ=60)
                                     CAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCT                                                         <  SRR4302540.3860244/94‑1 (MQ=60)
                                     CAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCT                                                         >  SRR4302540.1316042/1‑94 (MQ=60)
                                     CAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCCCCGGTTGATTTAGCGTATCGCT                                                         >  SRR4302540.1316038/1‑94 (MQ=60)
                                      AGCATGAAGACTGCACAGGACCGTCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTT                                                        >  SRR4302540.9982820/1‑94 (MQ=60)
                                      AGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTT                                                        <  SRR4302540.9947659/94‑1 (MQ=60)
                                      AGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTT                                                        >  SRR4302540.4113516/1‑94 (MQ=60)
                                       GCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTT                                                       >  SRR4302540.3752557/1‑94 (MQ=60)
                                        CATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTA                                                      >  SRR4302540.8009383/1‑94 (MQ=60)
                                        CATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTA                                                      >  SRR4302540.8983974/1‑94 (MQ=60)
                                         ATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAT                                                     >  SRR4302540.8723576/1‑94 (MQ=60)
                                         ATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAT                                                     >  SRR4302540.617360/1‑94 (MQ=60)
                                         ATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAT                                                     >  SRR4302540.209606/1‑94 (MQ=60)
                                         ATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAT                                                     >  SRR4302540.4707165/1‑94 (MQ=60)
                                         ATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAT                                                     >  SRR4302540.341070/1‑94 (MQ=60)
                                         ATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGCTGATTTAGCGTATCGCTTTAT                                                     >  SRR4302540.10122556/1‑94 (MQ=60)
                                          TGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATG                                                    <  SRR4302540.4555621/94‑1 (MQ=60)
                                          TGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATG                                                    <  SRR4302540.8351565/94‑1 (MQ=60)
                                           GAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGC                                                   <  SRR4302540.412278/94‑1 (MQ=60)
                                            AAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCG                                                  >  SRR4302540.494280/1‑94 (MQ=60)
                                             AGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGA                                                 >  SRR4302540.8852634/1‑94 (MQ=60)
                                             AGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGA                                                 <  SRR4302540.2952936/94‑1 (MQ=60)
                                             AGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGA                                                 <  SRR4302540.6347980/94‑1 (MQ=60)
                                              GACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAA                                                <  SRR4302540.1156286/94‑1 (MQ=60)
                                               ACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAA                                               <  SRR4302540.1576043/94‑1 (MQ=60)
                                               ACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAA                                               <  SRR4302540.3073075/94‑1 (MQ=60)
                                               ACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAA                                               >  SRR4302540.2068666/1‑94 (MQ=60)
                                                CTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAG                                              >  SRR4302540.5648489/1‑94 (MQ=60)
                                                CTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAG                                              >  SRR4302540.6458380/1‑94 (MQ=60)
                                                 TGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGG                                             <  SRR4302540.2906181/94‑1 (MQ=60)
                                                   CACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCG                                           <  SRR4302540.1973036/94‑1 (MQ=60)
                                                    ACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGA                                          >  SRR4302540.1880895/1‑94 (MQ=60)
                                                    ACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGA                                          >  SRR4302540.6176281/1‑94 (MQ=60)
                                                     CAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGAT                                         <  SRR4302540.393796/94‑1 (MQ=60)
                                                     CAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGAT                                         >  SRR4302540.744309/1‑94 (MQ=60)
                                                      AGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATG                                        >  SRR4302540.3270661/1‑94 (MQ=60)
                                                      AGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATG                                        >  SRR4302540.4763283/1‑94 (MQ=60)
                                                          CCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAT                                    >  SRR4302540.3193837/1‑94 (MQ=60)
                                                          CCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAT                                    <  SRR4302540.4075088/94‑1 (MQ=60)
                                                           CATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATT                                   >  SRR4302540.2816685/1‑94 (MQ=60)
                                                            ATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTG                                  <  SRR4302540.1388084/94‑1 (MQ=60)
                                                            ATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTG                                  >  SRR4302540.4387622/1‑94 (MQ=60)
                                                            ATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTG                                  >  SRR4302540.3042075/1‑94 (MQ=60)
                                                             TCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGT                                 <  SRR4302540.8778534/94‑1 (MQ=60)
                                                             TCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGT                                 >  SRR4302540.4553772/1‑94 (MQ=60)
                                                             TCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGT                                 <  SRR4302540.5678988/94‑1 (MQ=60)
                                                             TCAGCGGATCGATGGCAGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGT                                 <  SRR4302540.7989338/94‑1 (MQ=60)
                                                              CAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTT                                <  SRR4302540.4009252/94‑1 (MQ=60)
                                                                GCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGGAATTGTTCG                              >  SRR4302540.3994375/1‑94 (MQ=60)
                                                                GCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCG                              <  SRR4302540.4767333/94‑1 (MQ=60)
                                                                GCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCG                              >  SRR4302540.6292912/1‑94 (MQ=60)
                                                                 CGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGG                             >  SRR4302540.6366306/1‑94 (MQ=60)
                                                                 CGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGG                             >  SRR4302540.1434849/1‑94 (MQ=60)
                                                                 CGGATCGATGGCGGCTACTTTCTGGTGGGACCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGCTCGG                             >  SRR4302540.6366307/1‑94 (MQ=60)
                                                                  GGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGG                            >  SRR4302540.1684656/1‑94 (MQ=60)
                                                                  GGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGG                            >  SRR4302540.1948153/1‑94 (MQ=60)
                                                                   GATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGC                           >  SRR4302540.3562255/1‑94 (MQ=60)
                                                                   GATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGA                           >  SRR4302540.865662/1‑94 (MQ=60)
                                                                   GATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGA                           <  SRR4302540.7934320/94‑1 (MQ=60)
                                                                    ATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGAC                          <  SRR4302540.3624207/94‑1 (MQ=60)
                                                                    ATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGAC                          <  SRR4302540.2721032/94‑1 (MQ=60)
                                                                     TCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGcag                         >  SRR4302540.1387257/1‑91 (MQ=60)
                                                                     TCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACG                         >  SRR4302540.1387258/1‑94 (MQ=60)
                                                                     TCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACG                         >  SRR4302540.12575/1‑94 (MQ=60)
                                                                      CGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGC                        >  SRR4302540.5453937/1‑94 (MQ=60)
                                                                        ATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCAC                      <  SRR4302540.9748180/94‑1 (MQ=60)
                                                                        ATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGGATCGCTTTATGCGCAAGGCGATGAAATTGTTCGGGACGCAC                      >  SRR4302540.883130/1‑94 (MQ=60)
                                                                           gcttctatTTTCTGGTGGGCCTGTGCAATTATCGCCGGTTGAGTTAGCGTATCACTTTGTGCGAAAGGCGATGAAATTGTTCGGGACGCACTCT                   <  SRR4302540.8220567/86‑1 (MQ=60)
                                                                           GCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCT                   <  SRR4302540.8220568/94‑1 (MQ=60)
                                                                            CGTCTTCTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTT                  <  SRR4302540.1045316/94‑1 (MQ=60)
                                                                            CGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTT                  >  SRR4302540.4022082/1‑94 (MQ=60)
                                                                             GGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTC                 >  SRR4302540.5271246/1‑94 (MQ=60)
                                                                             GGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTC                 >  SRR4302540.9564359/1‑94 (MQ=60)
                                                                              GCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCG                >  SRR4302540.1106491/1‑94 (MQ=60)
                                                                               CTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGG               <  SRR4302540.712499/94‑1 (MQ=60)
                                                                                TACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGG              <  SRR4302540.4709855/94‑1 (MQ=60)
                                                                                 ACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGC             <  SRR4302540.5905760/94‑1 (MQ=60)
                                                                                 ACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGC             >  SRR4302540.2067518/1‑94 (MQ=60)
                                                                                  CTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCC            <  SRR4302540.5129131/94‑1 (MQ=60)
                                                                                   TTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCT           <  SRR4302540.8135998/94‑1 (MQ=60)
                                                                                       TGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCAT       <  SRR4302540.3373365/94‑1 (MQ=60)
                                                                                        GGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATT      >  SRR4302540.1299529/1‑94 (MQ=60)
                                                                                         GTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT     <  SRR4302540.5462336/94‑1 (MQ=60)
                                                                                           GGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGAATCTCG   >  SRR4302540.1450380/1‑94 (MQ=60)
                                                                                            GGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGG  >  SRR4302540.10008921/1‑94 (MQ=60)
                                                                                            GGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGG  <  SRR4302540.5850256/94‑1 (MQ=60)
                                                                                             |                                                                                            
TCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGG  >  NC_000913/1478001‑1478186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑