Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,584,418	T→C	I31M (ATA→ATG)	b1501 ←	putative oxidoreductase major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,584,418	0	T	C	92.5%	658.4 / 31.0	239	I31M (ATA→ATG)	b1501	putative oxidoreductase major subunit
Reads supporting (aligned to +/- strand): ref base T (9/9); new base C (125/96); total (134/105)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

Only 100 of 252 total aligned reads displayed.

GCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTC  >  NC_000913/1584329‑1584508

gCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGcata                                                                                         <  1:7199383/93‑1 (MQ=255)

 cACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCatat                                                                                        >  1:4215702/1‑93 (MQ=255)

 cACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCatat                                                                                        >  2:7550404/1‑93 (MQ=255)

  aCTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATc                                                                                       >  1:8540621/1‑93 (MQ=255)

   cTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATcc                                                                                      >  2:6523544/1‑93 (MQ=255)

    tgtgCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCa                                                                                     >  1:4379512/1‑93 (MQ=255)

     gtgCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCAt                                                                                    >  1:8864580/1‑93 (MQ=255)

       gCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCt                                                                                  <  1:1934382/93‑1 (MQ=255)

       gCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCt                                                                                  <  1:2688810/93‑1 (MQ=255)

         ttAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGc                                                                                >  2:4645875/1‑93 (MQ=255)

         ttAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGc                                                                                <  1:4657800/93‑1 (MQ=255)

          tAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCt                                                                               >  1:7165306/1‑93 (MQ=255)

          tAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCt                                                                               >  2:5963528/1‑93 (MQ=255)

          tAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCt                                                                               <  2:9312141/93‑1 (MQ=255)

           aGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCtt                                                                              >  2:1683446/1‑93 (MQ=255)

            ggATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTa                                                                             <  1:9264882/93‑1 (MQ=255)

             gATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTAc                                                                            >  1:1998190/1‑93 (MQ=255)

             gATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTAc                                                                            <  2:5344854/93‑1 (MQ=255)

             gATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTAc                                                                            <  1:5860662/93‑1 (MQ=255)

              aTCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACg                                                                           >  2:8165213/1‑93 (MQ=255)

              aTCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACg                                                                           >  1:2569797/1‑93 (MQ=255)

               tCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGt                                                                          >  2:7078080/1‑93 (MQ=255)

                cTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTa                                                                         <  2:7314637/93‑1 (MQ=255)

                 tGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTAc                                                                        <  2:213470/93‑1 (MQ=255)

                 tGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTAc                                                                        <  1:4733695/93‑1 (MQ=255)

                  ggCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACt                                                                       >  2:7936982/1‑93 (MQ=255)

                      aTGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCAt                                                                   >  1:9597140/1‑93 (MQ=255)

                       tGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGTGCATATCCATCTGCTTACGTACTGCAtt                                                                  >  1:7019597/1‑93 (MQ=255)

                       tGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCAtt                                                                  <  1:6491837/93‑1 (MQ=255)

                        gCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTc                                                                 <  1:9699763/93‑1 (MQ=255)

                         cacaACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCg                                                                >  1:8017966/1‑93 (MQ=255)

                           caACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCt                                                              <  2:5047402/93‑1 (MQ=255)

                            aaCCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTa                                                             >  1:597371/1‑93 (MQ=255)

                             aCCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTAc                                                            >  2:2746408/1‑93 (MQ=255)

                             aCCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTAc                                                            <  1:2151109/93‑1 (MQ=255)

                             aCCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTAc                                                            >  2:90953/1‑93 (MQ=255)

                                 ggACAGTCAAAGGCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCAATCGCTACGGAt                                                        >  2:1952674/1‑93 (MQ=255)

                                      gTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAAc                                                   >  1:686192/1‑93 (MQ=255)

                                       tCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACa                                                  <  1:51218/93‑1 (MQ=255)

                                         aaaGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGc                                                <  1:1770301/93‑1 (MQ=255)

                                          aaGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCa                                               <  2:1808647/93‑1 (MQ=255)

                                            gCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCAcc                                             <  1:1644313/93‑1 (MQ=255)

                                             cccTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCAccc                                            >  2:7119367/1‑93 (MQ=255)

                                              ccTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCAcccc                                           <  2:9854337/93‑1 (MQ=255)

                                                tctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCaa                                         >  2:3006642/1‑93 (MQ=255)

                                                tctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCaa                                         <  2:5154032/93‑1 (MQ=255)

                                                tctGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCaa                                         >  2:761015/1‑93 (MQ=255)

                                                  tGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGCTTTAACAGCACCCCAacc                                       >  1:4601081/1‑93 (MQ=255)

                                                  tGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAacc                                       <  2:3511863/93‑1 (MQ=255)

                                                   ggCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAacca                                      >  2:6953989/1‑93 (MQ=255)

                                                    gCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccac                                     >  2:1377011/1‑93 (MQ=255)

                                                    gCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccac                                     <  1:3221846/93‑1 (MQ=255)

                                                    gCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccac                                     <  1:3090004/93‑1 (MQ=255)

                                                    gCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccac                                     >  1:7784901/1‑93 (MQ=255)

                                                    gCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccac                                     <  1:8298595/93‑1 (MQ=255)

                                                     cTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccacc                                    <  2:9526333/93‑1 (MQ=255)

                                                     cTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAaccacc                                    <  1:8502320/93‑1 (MQ=255)

                                                      ttATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCt                                   >  2:4186602/1‑93 (MQ=255)

                                                      ttATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCt                                   >  2:8330154/1‑93 (MQ=255)

                                                       tATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTg                                  <  2:9018424/93‑1 (MQ=255)

                                                       tATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTg                                  >  2:6869065/1‑93 (MQ=255)

                                                       tATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTg                                  <  2:4708671/93‑1 (MQ=255)

                                                       tATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTg                                  >  1:5652973/1‑93 (MQ=255)

                                                       tATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTg                                  >  1:8187234/1‑93 (MQ=255)

                                                        aTTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTgc                                 <  1:1075745/93‑1 (MQ=255)

                                                        aTTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTgc                                 <  1:6923820/93‑1 (MQ=255)

                                                         ttCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTgca                                >  1:6266251/1‑93 (MQ=255)

                                                         ttCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTgca                                >  1:7338056/1‑93 (MQ=255)

                                                          tCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTgcag                               >  2:6270358/1‑93 (MQ=255)

                                                           cATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCCCCCCAACCACCTgcagc                              <  1:4195198/93‑1 (MQ=255)

                                                               tCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCAccc                          >  2:8267512/1‑93 (MQ=255)

                                                                cAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCt                         >  1:659505/1‑93 (MQ=255)

                                                                cAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCt                         >  1:7425374/1‑93 (MQ=255)

                                                                cAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCt                         >  2:2271700/1‑93 (MQ=255)

                                                                 aaaCATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTg                        >  2:6207584/1‑93 (MQ=255)

                                                                  aaCATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTgg                       >  2:4931758/1‑93 (MQ=255)

                                                                    cATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTTCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTa                     <  1:1232549/93‑1 (MQ=255)

                                                                    cATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTa                     <  1:7525878/93‑1 (MQ=255)

                                                                    cATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTa                     <  1:4447675/93‑1 (MQ=255)

                                                                    cATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTa                     <  2:3141736/93‑1 (MQ=255)

                                                                     aTGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAg                    >  1:3317294/1‑93 (MQ=255)

                                                                      tGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAgg                   >  2:455346/1‑93 (MQ=255)

                                                                       ggCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGa                  <  1:5570582/93‑1 (MQ=255)

                                                                         cAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGAtt                <  1:3731027/93‑1 (MQ=255)

                                                                           aTAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCa              >  2:4062633/1‑93 (MQ=255)

                                                                           aTAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCa              >  1:3949943/1‑93 (MQ=255)

                                                                            tAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCaa             >  1:9376435/1‑93 (MQ=255)

                                                                             aaCATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCATCCACCTGCAGCACCCTGGTAGGATTCAAt            <  2:6560810/93‑1 (MQ=255)

                                                                             aaCATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAt            <  2:7382526/93‑1 (MQ=255)

                                                                             aaCATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAt            <  1:2437033/93‑1 (MQ=255)

                                                                             aaCATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAt            >  2:9695852/1‑93 (MQ=255)

                                                                              aCATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAtt           >  1:7066714/1‑93 (MQ=255)

                                                                              aCATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAtt           >  2:8846208/1‑93 (MQ=255)

                                                                               cATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCACttt          >  1:7684225/1‑93 (MQ=255)

                                                                                aTCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAtttt         >  1:6180297/1‑93 (MQ=255)

                                                                                  ccTGGCGCATATCCATCTTCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAtttttt       <  1:5343304/93‑1 (MQ=255)

                                                                                   cTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAAttttttt      >  1:3118624/1‑93 (MQ=255)

                                                                                     ggCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTttct    <  2:5907728/93‑1 (MQ=255)

                                                                                     ggCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTttct    <  2:2609991/93‑1 (MQ=255)

                                                                                      gcgcATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTttctt   >  2:3292842/1‑93 (MQ=255)

GCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTC  >  NC_000913/1584329‑1584508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATC  >  NC_000913/1584327‑1584511

ACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCAT                                                                                             <  SRR4302540.2671264/94‑1 (MQ=60)

 CGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATA                                                                                            >  SRR4302540.7919238/1‑94 (MQ=60)

  GCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATAT                                                                                           <  SRR4302540.3136021/94‑1 (MQ=60)

  GCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATAT                                                                                           >  SRR4302540.4261601/1‑94 (MQ=60)

  GCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATAT                                                                                           >  SRR4302540.4261600/1‑94 (MQ=60)

  GCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATAT                                                                                           <  SRR4302540.7279645/94‑1 (MQ=60)

   CACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATC                                                                                          >  SRR4302540.2638169/1‑94 (MQ=60)

   CACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATC                                                                                          >  SRR4302540.8639857/1‑94 (MQ=60)

   CACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATC                                                                                          >  SRR4302540.8639856/1‑94 (MQ=60)

    CCTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCC                                                                                         <  SRR4302540.4753195/94‑1 (MQ=60)

    ACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTTTTCATGTCAAACATGGCAATAACATCCTGGCGCATATCC                                                                                         >  SRR4302540.3720190/1‑94 (MQ=60)

    ACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCC                                                                                         <  SRR4302540.4753193/94‑1 (MQ=60)

    ACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCC                                                                                         >  SRR4302540.6990218/1‑94 (MQ=60)

    ACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCC                                                                                         <  SRR4302540.54522/94‑1 (MQ=60)

     CTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCA                                                                                        >  SRR4302540.4427060/1‑94 (MQ=60)

      TGTGCTTAGGATCTGGCCATGCACATCCCGGACAGTCAAAGCCTTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCAT                                                                                       <  SRR4302540.717831/94‑1 (MQ=60)

      TGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCAT                                                                                       >  SRR4302540.8967155/1‑94 (MQ=60)

      TGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCAT                                                                                       <  SRR4302540.5618067/94‑1 (MQ=60)

       GTGCTTAGGATCTCGCCATGCACAACACGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATC                                                                                      <  SRR4302540.4080875/94‑1 (MQ=60)

        TGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCT                                                                                     <  SRR4302540.2089415/94‑1 (MQ=60)

         GCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTG                                                                                    <  SRR4302540.1955461/94‑1 (MQ=60)

         GCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTG                                                                                    <  SRR4302540.2717865/94‑1 (MQ=60)

           TTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCT                                                                                  <  SRR4302540.4707653/94‑1 (MQ=60)

           TTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCT                                                                                  >  SRR4302540.7245189/1‑94 (MQ=60)

           TTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCT                                                                                  <  SRR4302540.8734331/94‑1 (MQ=60)

            TAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTT                                                                                 >  SRR4302540.4311836/1‑94 (MQ=60)

            TAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTT                                                                                 >  SRR4302540.3209572/1‑94 (MQ=60)

              GGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTAC                                                                               <  SRR4302540.9375706/94‑1 (MQ=60)

              GGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTAC                                                                               >  SRR4302540.2019835/1‑94 (MQ=60)

               GATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACG                                                                              >  SRR4302540.2597506/1‑94 (MQ=60)

               GATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACG                                                                              <  SRR4302540.1239637/94‑1 (MQ=60)

               GATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACG                                                                              <  SRR4302540.5925471/94‑1 (MQ=60)

                ATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGT                                                                             <  SRR4302540.9753143/94‑1 (MQ=60)

                ATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGT                                                                             >  SRR4302540.6322683/1‑94 (MQ=60)

                   TGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACT                                                                          <  SRR4302540.4784240/94‑1 (MQ=60)

                    GGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTG                                                                         <  SRR4302540.3302849/94‑1 (MQ=60)

                      CCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCA                                                                       >  SRR4302540.2445673/1‑94 (MQ=60)

                       CATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCAT                                                                      >  SRR4302540.9710378/1‑94 (MQ=60)

                        ATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGTGCATATCCATCTGCTTACGTACTGCATT                                                                     >  SRR4302540.7097562/1‑94 (MQ=60)

                        ATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATT                                                                     >  SRR4302540.7097560/1‑94 (MQ=60)

                        ATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATT                                                                     >  SRR4302540.2012775/1‑94 (MQ=60)

                        ATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATT                                                                     >  SRR4302540.4499316/1‑94 (MQ=60)

                        ATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATT                                                                     >  SRR4302540.100770/1‑94 (MQ=60)

                        ATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATT                                                                     >  SRR4302540.2923735/1‑94 (MQ=60)

                         TGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTC                                                                    <  SRR4302540.6564034/94‑1 (MQ=60)

                         TGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTC                                                                    >  SRR4302540.2567538/1‑94 (MQ=60)

                          GCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCG                                                                   <  SRR4302540.9813803/94‑1 (MQ=60)

                          GCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCG                                                                   >  SRR4302540.8111218/1‑94 (MQ=60)

                           CACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGC                                                                  <  SRR4302540.8421991/94‑1 (MQ=60)

                             CAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTA                                                                >  SRR4302540.603784/1‑94 (MQ=60)

                              AACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTAC                                                               <  SRR4302540.4445022/94‑1 (MQ=60)

                              AACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTAC                                                               >  SRR4302540.396856/1‑94 (MQ=60)

                               ACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACG                                                              <  SRR4302540.2174240/94‑1 (MQ=60)

                                CCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGG                                                             >  SRR4302540.9467104/1‑94 (MQ=60)

                                 CCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGA                                                            <  SRR4302540.7503199/94‑1 (MQ=60)

                                  CGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGAT                                                           >  SRR4302540.4688616/1‑94 (MQ=60)

                                   GGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATT                                                          >  SRR4302540.4156723/1‑94 (MQ=60)

                                    GACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTT                                                         <  SRR4302540.1740027/94‑1 (MQ=60)

                                       AGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAAC                                                      >  SRR4302540.693555/1‑94 (MQ=60)

                                         TCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAG                                                    <  SRR4302540.720138/94‑1 (MQ=60)

                                         TCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAG                                                    <  SRR4302540.51732/94‑1 (MQ=60)

                                          CAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGC                                                   >  SRR4302540.888254/1‑94 (MQ=60)

                                           AAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCA                                                  <  SRR4302540.9556881/94‑1 (MQ=60)

                                           AAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCA                                                  <  SRR4302540.1789509/94‑1 (MQ=60)

                                            AAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCAC                                                 <  SRR4302540.6704951/94‑1 (MQ=60)

                                            AAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCAC                                                 <  SRR4302540.6704950/94‑1 (MQ=60)

                                            AAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCAC                                                 >  SRR4302540.1378819/1‑94 (MQ=60)

                                             AGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACC                                                >  SRR4302540.2501135/1‑94 (MQ=60)

                                             AGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACC                                                >  SRR4302540.6404108/1‑94 (MQ=60)

                                              GCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCC                                               <  SRR4302540.1662275/94‑1 (MQ=60)

                                                CCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCA                                             >  SRR4302540.7867344/1‑94 (MQ=60)

                                                CCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCA                                             <  SRR4302540.7844918/94‑1 (MQ=60)

                                                CCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCA                                             >  SRR4302540.7590147/1‑94 (MQ=60)

                                                   CTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGCTTTAACAGCACCCCAACC                                          >  SRR4302540.4650528/1‑94 (MQ=60)

                                                   CTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCGCAACC                                          >  SRR4302540.5759795/1‑94 (MQ=60)

                                                   CTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACC                                          >  SRR4302540.5759793/1‑94 (MQ=60)

                                                     GGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCAC                                        >  SRR4302540.7875896/1‑94 (MQ=60)

                                                     GGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCAC                                        >  SRR4302540.5965794/1‑94 (MQ=60)

                                                      GCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACC                                       <  SRR4302540.3256861/94‑1 (MQ=60)

                                                      GCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACC                                       <  SRR4302540.8394952/94‑1 (MQ=60)

                                                      GCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACC                                       <  SRR4302540.3123904/94‑1 (MQ=60)

                                                      GCTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACC                                       <  SRR4302540.9226519/94‑1 (MQ=60)

                                                       CTTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCT                                      <  SRR4302540.8601119/94‑1 (MQ=60)

                                                        TTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTG                                     >  SRR4302540.8282349/1‑94 (MQ=60)

                                                        TTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTG                                     >  SRR4302540.4620168/1‑94 (MQ=60)

                                                        TTATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTG                                     >  SRR4302540.5715210/1‑94 (MQ=60)

                                                          tatcgtggccaatcTGGCAAGAACAGCCTGGCGCATAGCCATCGGCTGACGTACCGCATGCCCTACGGATTTAACAGCACCCCAACCACCTGCA                                   <  SRR4302540.7000881/80‑1 (MQ=28)

                                                          ATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCA                                   <  SRR4302540.1086741/94‑1 (MQ=60)

                                                          ATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCA                                   >  SRR4302540.7419946/1‑94 (MQ=60)

                                                          ATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCA                                   <  SRR4302540.7000872/94‑1 (MQ=60)

                                                          ATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCA                                   <  SRR4302540.5650848/94‑1 (MQ=60)

                                                          ATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCA                                   >  SRR4302540.6335965/1‑94 (MQ=60)

                                                          ATTCATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCA                                   >  SRR4302540.6335967/1‑94 (MQ=60)

                                                             CATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCCCCCCAACCACCTGCAGCA                                <  SRR4302540.4240833/94‑1 (MQ=60)

                                                              ATGTCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCAC                               <  SRR4302540.4388119/94‑1 (MQ=60)

                                                                 TCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCT                            >  SRR4302540.7508090/1‑94 (MQ=60)

                                                                 TCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCT                            >  SRR4302540.666543/1‑94 (MQ=60)

                                                                 TCAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCT                            <  SRR4302540.7273231/94‑1 (MQ=60)

                                                                  CAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTG                           >  SRR4302540.7407715/1‑94 (MQ=60)

                                                                  CAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTGCTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTG                           >  SRR4302540.3227017/1‑94 (MQ=60)

                                                                  CAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTG                           <  SRR4302540.930457/94‑1 (MQ=60)

                                                                  CAAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTG                           <  SRR4302540.8238504/94‑1 (MQ=60)

                                                                   AAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGG                          <  SRR4302540.5258486/94‑1 (MQ=60)

                                                                   AAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGG                          >  SRR4302540.5353839/1‑94 (MQ=60)

                                                                   AAACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGG                          >  SRR4302540.707931/1‑94 (MQ=60)

                                                                     ACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTA                        >  SRR4302540.1938033/1‑94 (MQ=60)

                                                                     ACATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTA                        >  SRR4302540.6645595/1‑94 (MQ=60)

                                                                      CATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTTCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAG                       <  SRR4302540.1245133/94‑1 (MQ=60)

                                                                      CATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAG                       >  SRR4302540.3353668/1‑94 (MQ=60)

                                                                      CATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAG                       <  SRR4302540.7610089/94‑1 (MQ=60)

                                                                      CATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAG                       <  SRR4302540.7610088/94‑1 (MQ=60)

                                                                      CATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAG                       <  SRR4302540.4495879/94‑1 (MQ=60)

                                                                      CATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAG                       <  SRR4302540.5055042/94‑1 (MQ=60)

                                                                       ATGGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGG                      <  SRR4302540.6163378/94‑1 (MQ=60)

                                                                         GGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGAT                    <  SRR4302540.5631917/94‑1 (MQ=60)

                                                                         GGCAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGAT                    >  SRR4302540.7396321/1‑94 (MQ=60)

                                                                           CAATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTC                  <  SRR4302540.3771731/94‑1 (MQ=60)

                                                                            AATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCA                 >  SRR4302540.3992588/1‑94 (MQ=60)

                                                                             ATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAT                >  SRR4302540.8271382/1‑94 (MQ=60)

                                                                             ATAACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAA                >  SRR4302540.9488055/1‑94 (MQ=60)

                                                                               AACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATT              >  SRR4302540.135460/1‑94 (MQ=60)

                                                                               AACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATT              >  SRR4302540.7145309/1‑94 (MQ=60)

                                                                               AACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATT              >  SRR4302540.287815/1‑94 (MQ=60)

                                                                               AACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATT              <  SRR4302540.2463275/94‑1 (MQ=60)

                                                                                ACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCACTTT             >  SRR4302540.7771333/1‑94 (MQ=60)

                                                                                ACATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTT             <  SRR4302540.8531452/94‑1 (MQ=60)

                                                                                 CATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTT            >  SRR4302540.6249141/1‑94 (MQ=60)

                                                                                 CATCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTT            <  SRR4302540.3721623/94‑1 (MQ=60)

                                                                                   TCCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTT          <  SRR4302540.8372945/94‑1 (MQ=60)

                                                                                    CCTGGCGCATATCCATCTTCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTT         <  SRR4302540.5401912/94‑1 (MQ=60)

                                                                                    CCTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTT         >  SRR4302540.3152715/1‑94 (MQ=60)

                                                                                     CTGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTC        <  SRR4302540.9709073/94‑1 (MQ=60)

                                                                                      TGGCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCT       <  SRR4302540.6173950/94‑1 (MQ=60)

                                                                                        GCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTC     <  SRR4302540.7115124/94‑1 (MQ=60)

                                                                                        GCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTC     <  SRR4302540.1900966/94‑1 (MQ=60)

                                                                                        GCGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTC     <  SRR4302540.7671559/94‑1 (MQ=60)

                                                                                         CGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCA    <  SRR4302540.2172655/94‑1 (MQ=60)

                                                                                         CGCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCA    <  SRR4302540.7746012/94‑1 (MQ=60)

                                                                                          GCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCAT   <  SRR4302540.6594462/94‑1 (MQ=60)

                                                                                          GCATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCAT   >  SRR4302540.1913918/1‑94 (MQ=60)

                                                                                           CATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATC  <  SRR4302540.9355974/94‑1 (MQ=60)

ACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTATTCATGTCAAACATGGCAATAACATCCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATC  >  NC_000913/1584327‑1584511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`