Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,712,211 A→G V473V (GTA→GTG)  ydgR → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,2110AG99.6% 719.4 / ‑5.1 220V473V (GTA→GTG) ydgRputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (136/83);  total (136/84)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

Only 100 of 225 total aligned reads displayed.

GCCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCA  >  NC_000913/1712121‑1712301
                                                                                          |                                                                                          
gCCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCt                                                                                          >  2:2762217/1‑93 (MQ=255)
 ccGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTg                                                                                         >  1:4575646/1‑93 (MQ=255)
  cGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGa                                                                                        <  1:6224645/93‑1 (MQ=255)
  cGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGa                                                                                        <  2:4026431/93‑1 (MQ=255)
   ggATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGAt                                                                                       <  1:847308/93‑1 (MQ=255)
     aTAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGAtgc                                                                                     >  1:6491651/1‑93 (MQ=255)
       aaCGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGAtgctg                                                                                   >  1:2272084/1‑93 (MQ=255)
          gTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGctgctg                                                                                >  2:8147248/1‑93 (MQ=255)
          gTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGctgctg                                                                                >  2:5684477/1‑93 (MQ=255)
           ttACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGa                                                                               >  2:5563972/1‑93 (MQ=255)
           ttACCGATCCGCTGATGTCACTAGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGa                                                                               >  1:1489451/1‑93 (MQ=255)
            tACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGAc                                                                              >  1:4028792/1‑93 (MQ=255)
            tACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGAc                                                                              >  2:1455270/1‑93 (MQ=255)
            tACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGAc                                                                              <  2:9161647/93‑1 (MQ=255)
               cGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACcgc                                                                           >  1:6623383/1‑93 (MQ=255)
               cGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACcgc                                                                           >  2:4761172/1‑93 (MQ=255)
                gATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACcgcg                                                                          >  2:9522077/1‑93 (MQ=255)
                gATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACcgcg                                                                          >  1:2765407/1‑93 (MQ=255)
                  tCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGcc                                                                        <  2:1271762/93‑1 (MQ=255)
                   ccGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCg                                                                       >  2:9255797/1‑93 (MQ=255)
                     gCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGaa                                                                     >  2:8357604/1‑93 (MQ=255)
                          tGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGc                                                                <  1:3774937/93‑1 (MQ=255)
                           gTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCa                                                               >  2:6237718/1‑93 (MQ=255)
                            tCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCAc                                                              >  1:2502696/1‑93 (MQ=255)
                            tCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCAc                                                              <  1:1282464/93‑1 (MQ=255)
                            tCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCAc                                                              >  2:3432262/1‑93 (MQ=255)
                            tCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCAc                                                              >  1:8153501/1‑93 (MQ=255)
                             taCTGGAAGGCTATGGTCGCGTATTCTTGCAGATNGGAGTCGCTACTGCCGTTATTGCANTGCTGATGCNGCTGACCGCGCCGAAACTGCAcc                                                             <  2:8707174/92‑1 (MQ=255)
                              aCTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCg                                                            >  2:4591903/1‑93 (MQ=255)
                              aCTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCg                                                            >  2:5124106/1‑93 (MQ=255)
                               cTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATCCTGCTGACCGCGCCGAAACTGCACCGc                                                           >  2:2491386/1‑93 (MQ=255)
                                tGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCa                                                          <  1:688081/93‑1 (MQ=255)
                                   aaGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGa                                                       >  1:1028459/1‑93 (MQ=255)
                                   aaGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGa                                                       >  2:6676716/1‑93 (MQ=255)
                                   aaGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGa                                                       <  1:3010089/93‑1 (MQ=255)
                                    aGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGAc                                                      >  2:7744224/1‑93 (MQ=255)
                                    aGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGAc                                                      >  1:2574516/1‑93 (MQ=255)
                                     gTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACg                                                     >  1:3467587/1‑93 (MQ=255)
                                      tCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGc                                                    >  1:3383748/1‑93 (MQ=255)
                                      tCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGc                                                    <  1:7294820/93‑1 (MQ=255)
                                       cTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCa                                                   <  2:2718110/93‑1 (MQ=255)
                                        tATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAg                                                  <  1:6260901/93‑1 (MQ=255)
                                        tATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAg                                                  >  1:4376309/1‑93 (MQ=255)
                                        tATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGATGCTGACCGCGCCGAAACTGCACCGCATGACGCAg                                                  <  2:4520579/93‑1 (MQ=255)
                                          tGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGa                                                <  2:7638844/93‑1 (MQ=255)
                                          tGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGa                                                >  1:9502835/1‑93 (MQ=255)
                                          tGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGa                                                <  1:2203244/93‑1 (MQ=255)
                                           ggTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGAt                                               <  1:5975851/93‑1 (MQ=255)
                                             tCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGTTGATGCTGCTGACCGCGCCGAAAGTGCACCGCATGACGCGGGATGa                                             >  1:2891769/1‑93 (MQ=255)
                                             tCGCATATGCTTACAGATAGGTGGCTCTACTGCGGTTATTGCAGTGCTGAAGCGGCTGACCGTGTCGAAACGGCACCGCATGACGCAGCATGa                                             <  2:5798759/93‑1 (MQ=255)
                                              cgcgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGAc                                            >  1:8107100/1‑93 (MQ=255)
                                               gcgTATTCGTGCAGATTGGTGTCGCTGCTGCCGTTATTGCAGTGCTGATGCTGCTGCCCGCGCCGAAACTGCACCGCATGACGCAGGATGACg                                           <  1:3605183/93‑1 (MQ=255)
                                                cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGc                                          >  1:7753619/1‑93 (MQ=255)
                                                cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGc                                          >  2:4030931/1‑93 (MQ=255)
                                                cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGc                                          <  2:1036362/93‑1 (MQ=255)
                                                  tATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTg                                        >  2:9665893/1‑93 (MQ=255)
                                                   aTTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGc                                       >  2:5342881/1‑93 (MQ=255)
                                                   aTTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGc                                       >  2:3939091/1‑93 (MQ=255)
                                                   aTTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGc                                       <  2:3099372/93‑1 (MQ=255)
                                                    ttCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCa                                      <  1:7259743/93‑1 (MQ=255)
                                                    ttCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCa                                      >  2:3296269/1‑93 (MQ=255)
                                                    ttCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACAGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCa                                      >  2:289135/1‑93 (MQ=255)
                                                       ttGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGAc                                   <  1:5516940/93‑1 (MQ=255)
                                                        tGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACa                                  <  1:9283389/93‑1 (MQ=255)
                                                          cAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACaaa                                >  1:1944590/1‑93 (MQ=255)
                                                           aGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAg                               <  2:10047476/93‑1 (MQ=255)
                                                           aGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAg                               >  2:7884682/1‑93 (MQ=255)
                                                            gATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgc                              >  2:305955/1‑93 (MQ=255)
                                                            gATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgc                              <  2:2084687/93‑1 (MQ=255)
                                                            gATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgc                              <  2:9586196/93‑1 (MQ=255)
                                                            gATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgc                              <  1:9952309/93‑1 (MQ=255)
                                                              ttGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgcgg                            >  1:1249008/1‑93 (MQ=255)
                                                                ggTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgcggcg                          >  1:5077925/1‑93 (MQ=255)
                                                                ggTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAgcggcg                          >  1:667876/1‑93 (MQ=255)
                                                                 gtgtCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGa                         >  2:7092937/1‑93 (MQ=255)
                                                                   gtCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGaaa                       <  1:4205536/93‑1 (MQ=255)
                                                                     cGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACTGCATGACGCAGGCTGCAGCTGAAGACAACGCGGCGAAagc                     >  1:6279685/1‑93 (MQ=255)
                                                                     cGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAagc                     <  1:9791929/93‑1 (MQ=255)
                                                                     cGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAagc                     <  1:5944795/93‑1 (MQ=255)
                                                                     cGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAagc                     <  2:1582723/93‑1 (MQ=255)
                                                                        tACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAagcagc                  >  1:6600238/1‑93 (MQ=255)
                                                                        tACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAagcagc                  <  2:2369431/93‑1 (MQ=255)
                                                                         aCTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGcc                 <  2:2465710/93‑1 (MQ=255)
                                                                          cTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCg                >  1:3363637/1‑93 (MQ=255)
                                                                          cTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCg                >  2:1368174/1‑93 (MQ=255)
                                                                          cTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCg                <  2:3740479/93‑1 (MQ=255)
                                                                           tGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGt               >  1:2921040/1‑93 (MQ=255)
                                                                            gCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTa              >  1:2103610/1‑93 (MQ=255)
                                                                            gCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTa              >  2:1676399/1‑93 (MQ=255)
                                                                            gCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCAAAAGCAGCCGTa              <  1:8575173/93‑1 (MQ=255)
                                                                              cGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGc            <  1:6939694/93‑1 (MQ=255)
                                                                               gTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCg           >  1:5658427/1‑93 (MQ=255)
                                                                               gTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCg           >  2:7732327/1‑93 (MQ=255)
                                                                                 tATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTa         >  1:8345702/1‑93 (MQ=255)
                                                                                  aTTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTaa        >  1:10012192/1‑93 (MQ=255)
                                                                                   ttGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTaaa       >  2:6877320/1‑93 (MQ=255)
                                                                                   ttGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTaaa       >  1:1676931/1‑93 (MQ=255)
                                                                                     gCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAAt     <  1:4842165/93‑1 (MQ=255)
                                                                                       aGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGTAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTc   <  1:7131143/93‑1 (MQ=255)
                                                                                       aGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTc   >  1:6232128/1‑93 (MQ=255)
                                                                                          |                                                                                          
GCCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCA  >  NC_000913/1712121‑1712301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGCCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGG  >  NC_000913/1712118‑1712303
                                                                                             |                                                                                            
cgtggCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCGATGGTTGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTG                                                                                              <  SRR4302540.2004165/89‑1 (MQ=60)
   GCCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTG                                                                                           >  SRR4302540.4625003/1‑94 (MQ=60)
     CGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGAT                                                                                         <  SRR4302540.6293876/94‑1 (MQ=60)
     CGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGAT                                                                                         <  SRR4302540.6293877/94‑1 (MQ=60)
     CGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGAT                                                                                         <  SRR4302540.1448789/94‑1 (MQ=60)
      GGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATG                                                                                        <  SRR4302540.856294/94‑1 (MQ=60)
      GGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATG                                                                                        >  SRR4302540.3413613/1‑94 (MQ=60)
      GGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATG                                                                                        <  SRR4302540.5149899/94‑1 (MQ=60)
      GGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATG                                                                                        <  SRR4302540.4525467/94‑1 (MQ=60)
       GATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGC                                                                                       >  SRR4302540.6563845/1‑94 (MQ=60)
       GATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGC                                                                                       >  SRR4302540.6684502/1‑94 (MQ=60)
        ATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCT                                                                                      >  SRR4302540.3179430/1‑94 (MQ=60)
        ATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCT                                                                                      >  SRR4302540.1154319/1‑94 (MQ=60)
         TAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTG                                                                                     >  SRR4302540.2296469/1‑94 (MQ=60)
         TAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTG                                                                                     >  SRR4302540.2296468/1‑94 (MQ=60)
             GTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGA                                                                                 <  SRR4302540.2922732/94‑1 (MQ=60)
             GTTACCGATCCGCTGATGTCACTAGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGA                                                                                 >  SRR4302540.1505826/1‑94 (MQ=60)
              TTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGAC                                                                                >  SRR4302540.4072237/1‑94 (MQ=60)
              TTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGAC                                                                                <  SRR4302540.754493/94‑1 (MQ=60)
               TACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACC                                                                               <  SRR4302540.8019016/94‑1 (MQ=60)
               TACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACC                                                                               >  SRR4302540.850364/1‑94 (MQ=60)
               TACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACC                                                                               <  SRR4302540.1973472/94‑1 (MQ=60)
                 CCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGC                                                                             >  SRR4302540.6696714/1‑94 (MQ=60)
                 CCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGC                                                                             <  SRR4302540.296920/94‑1 (MQ=60)
                  CGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCG                                                                            >  SRR4302540.2795287/1‑94 (MQ=60)
                    ATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCC                                                                          <  SRR4302540.2225970/94‑1 (MQ=60)
                        GCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAA                                                                      >  SRR4302540.7110674/1‑94 (MQ=60)
                          TGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACT                                                                    <  SRR4302540.6666373/94‑1 (MQ=60)
                           GATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTG                                                                   >  SRR4302540.6603914/1‑94 (MQ=60)
                            ATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGC                                                                  >  SRR4302540.2422911/1‑94 (MQ=60)
                             TGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCA                                                                 <  SRR4302540.3816122/94‑1 (MQ=60)
                             TGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCA                                                                 >  SRR4302540.2770802/1‑94 (MQ=60)
                              GTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCAC                                                                >  SRR4302540.2529702/1‑94 (MQ=60)
                              GTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCAC                                                                >  SRR4302540.8248166/1‑94 (MQ=60)
                               TCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACC                                                               <  SRR4302540.1295690/94‑1 (MQ=60)
                                CACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCG                                                              <  SRR4302540.701316/94‑1 (MQ=60)
                                  CTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCA                                                            >  SRR4302540.1694206/1‑94 (MQ=60)
                                  CTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGGGTCGCTACTGCCGTTATTGCAGGGCTGATGCTGCTGACCGCGCACAAACTGCTCCGCA                                                            >  SRR4302540.1897435/1‑94 (MQ=60)
                                   TGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCAT                                                           <  SRR4302540.695467/94‑1 (MQ=60)
                                    GGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATG                                                          >  SRR4302540.3821017/1‑94 (MQ=60)
                                     GAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTTCTGACCGTGCCGAAACTGCACCGCATGA                                                         >  SRR4302540.6011080/1‑94 (MQ=60)
                                     GAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGA                                                         >  SRR4302540.1038891/1‑94 (MQ=60)
                                      AAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGAC                                                        >  SRR4302540.923915/1‑94 (MQ=60)
                                      AAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGAC                                                        >  SRR4302540.2602266/1‑94 (MQ=60)
                                      AAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGAC                                                        <  SRR4302540.3043205/94‑1 (MQ=60)
                                       AGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACG                                                       >  SRR4302540.3505341/1‑94 (MQ=60)
                                        GTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGC                                                      >  SRR4302540.3420680/1‑94 (MQ=60)
                                        GTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGC                                                      >  SRR4302540.314708/1‑94 (MQ=60)
                                        GTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGC                                                      >  SRR4302540.8201902/1‑94 (MQ=60)
                                         TCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCA                                                     <  SRR4302540.7376233/94‑1 (MQ=60)
                                         TCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCA                                                     >  SRR4302540.6714272/1‑94 (MQ=60)
                                          CTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAG                                                    >  SRR4302540.4423829/1‑94 (MQ=60)
                                          CTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAG                                                    >  SRR4302540.8420598/1‑94 (MQ=60)
                                          CTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAG                                                    >  SRR4302540.9479675/1‑94 (MQ=60)
                                          CTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAG                                                    >  SRR4302540.6501546/1‑94 (MQ=60)
                                          CTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAG                                                    <  SRR4302540.10131263/94‑1 (MQ=60)
                                           TATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGG                                                   <  SRR4302540.6330557/94‑1 (MQ=60)
                                            ATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGA                                                  >  SRR4302540.9615435/1‑94 (MQ=60)
                                             TGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGAT                                                 <  SRR4302540.7294409/94‑1 (MQ=60)
                                             TGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGAT                                                 <  SRR4302540.2227048/94‑1 (MQ=60)
                                              GGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATG                                                <  SRR4302540.6042004/94‑1 (MQ=60)
                                               GTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGTTGATGCTGCTGACCGCGCCGAAAGTGCACCGCATGACGCGGGATGA                                               >  SRR4302540.2923287/1‑94 (MQ=60)
                                               GTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGA                                               >  SRR4302540.2923288/1‑94 (MQ=60)
                                               GTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGA                                               >  SRR4302540.3754871/1‑94 (MQ=60)
                                               GTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCGGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGA                                               <  SRR4302540.3667766/94‑1 (MQ=60)
                                                TCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGAC                                              >  SRR4302540.4460135/1‑94 (MQ=60)
                                                TCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGAC                                              >  SRR4302540.8201191/1‑94 (MQ=60)
                                                TCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGAC                                              <  SRR4302540.6826248/94‑1 (MQ=60)
                                                 CGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACG                                             <  SRR4302540.8519199/94‑1 (MQ=60)
                                                 CGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACG                                             >  SRR4302540.2546528/1‑94 (MQ=60)
                                                  GCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGC                                            >  SRR4302540.7844170/1‑94 (MQ=60)
                                                  GCGTATTCGTGCAGATTGGTGTCGCTGCTGCCGTTATTGCAGTGCTGATGCTGCTGCCCGCGCCGAAACTGCACCGCATGACGCAGGATGACGC                                            <  SRR4302540.3644580/94‑1 (MQ=60)
                                                    GTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTG                                          <  SRR4302540.7690137/94‑1 (MQ=60)
                                                    GTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTG                                          >  SRR4302540.2748416/1‑94 (MQ=60)
                                                     TATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGC                                         >  SRR4302540.6999399/1‑94 (MQ=60)
                                                      ATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCA                                        >  SRR4302540.9799581/1‑94 (MQ=60)
                                                       TTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAG                                       <  SRR4302540.7340751/94‑1 (MQ=60)
                                                          TTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACA                                    <  SRR4302540.5577705/94‑1 (MQ=60)
                                                          TTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACA                                    >  SRR4302540.4700519/1‑94 (MQ=60)
                                                           TGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAA                                   <  SRR4302540.9394340/94‑1 (MQ=60)
                                                            GCAGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAA                                  >  SRR4302540.1965758/1‑94 (MQ=60)
                                                              AGATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGC                                <  SRR4302540.2188824/94‑1 (MQ=60)
                                                               GATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCG                               >  SRR4302540.6142816/1‑94 (MQ=60)
                                                               GATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCG                               <  SRR4302540.10068488/94‑1 (MQ=60)
                                                               GATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCG                               >  SRR4302540.10062432/1‑94 (MQ=60)
                                                                ATTGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGG                              >  SRR4302540.1261764/1‑94 (MQ=60)
                                                                  TGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCG                            >  SRR4302540.675035/1‑94 (MQ=60)
                                                                  TGGTGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCG                            >  SRR4302540.5132826/1‑94 (MQ=60)
                                                                     TGTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA                         <  SRR4302540.8627548/94‑1 (MQ=60)
                                                                      GTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAG                        <  SRR4302540.4251306/94‑1 (MQ=60)
                                                                      GTCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAG                        <  SRR4302540.6299327/94‑1 (MQ=60)
                                                                       TCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACTGCATGACGCAGGCTGCAGCTGAAGACAACGCGGCGAAAGC                       >  SRR4302540.6349567/1‑94 (MQ=60)
                                                                       TCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGC                       >  SRR4302540.1264137/1‑94 (MQ=60)
                                                                       TCGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACAGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAAC                       >  SRR4302540.6349563/1‑94 (MQ=60)
                                                                        CGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCA                      <  SRR4302540.9906629/94‑1 (MQ=60)
                                                                        CGCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCA                      <  SRR4302540.6010510/94‑1 (MQ=60)
                                                                         GCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAG                     >  SRR4302540.1346570/1‑94 (MQ=60)
                                                                         GCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAG                     >  SRR4302540.4280586/1‑94 (MQ=60)
                                                                         GCTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAG                     <  SRR4302540.5945303/94‑1 (MQ=60)
                                                                          CTACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGC                    >  SRR4302540.6673328/1‑94 (MQ=60)
                                                                            ACTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCG                  >  SRR4302540.3400447/1‑94 (MQ=60)
                                                                             CTGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGT                 >  SRR4302540.2953047/1‑94 (MQ=60)
                                                                              TGCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTA                >  SRR4302540.2126193/1‑94 (MQ=60)
                                                                               GCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAG               <  SRR4302540.9675412/94‑1 (MQ=60)
                                                                               GCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAG               >  SRR4302540.7344076/1‑94 (MQ=60)
                                                                               GCCGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCAAAAGCAGCCGTAG               <  SRR4302540.8674812/94‑1 (MQ=60)
                                                                                 CGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCG             >  SRR4302540.10098932/1‑94 (MQ=60)
                                                                                 CGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCG             <  SRR4302540.7016939/94‑1 (MQ=60)
                                                                                 CGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCG             >  SRR4302540.5720734/1‑94 (MQ=60)
                                                                                 CGTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCG             <  SRR4302540.5608944/94‑1 (MQ=60)
                                                                                  GTTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGT            >  SRR4302540.4140355/1‑94 (MQ=60)
                                                                                  GTTATTGCAGGGCTGATGCTGCTGACCGCGCCGAAGCTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGT            <  SRR4302540.3463600/94‑1 (MQ=60)
                                                                                   TTATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTA           >  SRR4302540.8442566/1‑94 (MQ=60)
                                                                                    TATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAA          >  SRR4302540.10128767/1‑94 (MQ=60)
                                                                                     ATTGCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAA         >  SRR4302540.1695146/1‑94 (MQ=60)
                                                                                        GCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATT      <  SRR4302540.354574/94‑1 (MQ=60)
                                                                                        GCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATT      <  SRR4302540.4894025/94‑1 (MQ=60)
                                                                                        GCAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATT      <  SRR4302540.4894026/94‑1 (MQ=60)
                                                                                         CAGTGCTGATGCTGCTGACCTCGCCGAAACTGCACCGCATGACGCA‑GATGACGCTGCAGACAAAGCGGCGAAATCAGCCGTAGCGTAAAATTCA    >  SRR4302540.6301443/1‑94 (MQ=60)
                                                                                         CAGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTC     >  SRR4302540.6301442/1‑94 (MQ=60)
                                                                                          AGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGTAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCA    <  SRR4302540.7210580/94‑1 (MQ=60)
                                                                                          AGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCA    >  SRR4302540.806881/1‑94 (MQ=60)
                                                                                          AGTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCA    <  SRR4302540.5625241/94‑1 (MQ=60)
                                                                                           GTGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAG   >  SRR4302540.7582324/1‑94 (MQ=60)
                                                                                            TGCTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGG  >  SRR4302540.2251739/1‑94 (MQ=60)
                                                                                             |                                                                                            
TGTGCCGGATAACGTTACCGATCCGCTGATGTCACTGGAAGTCTATGGTCGCGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGG  >  NC_000913/1712118‑1712303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑