Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,779,044 A→G Q207R (CAG→CGG)  ydiR → putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,779,0440AG99.3% 461.1 / ‑5.0 147Q207R (CAG→CGG) ydiRputative flavoprotein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (68/78);  total (69/78)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

TCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGA  >  NC_000913/1778954‑1779133
                                                                                          |                                                                                         
 ctGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACGTGAGTAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGa                                                                                        <  2:3800021/93‑1 (MQ=255)
 ctGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGa                                                                                        >  1:7974541/1‑93 (MQ=255)
  tGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGag                                                                                       >  1:3809138/1‑92 (MQ=255)
  tGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGAt                                                                                       <  2:4255543/93‑1 (MQ=255)
  tGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGAt                                                                                       <  2:8084050/93‑1 (MQ=255)
   gTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATg                                                                                      <  2:4402869/93‑1 (MQ=255)
    tCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGGGCGGGATGa                                                                                     >  2:6401443/1‑93 (MQ=255)
     cgcgAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGAc                                                                                    <  1:1444681/93‑1 (MQ=255)
       cgAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCt                                                                                  >  2:9331068/1‑93 (MQ=255)
          aCGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTaaa                                                                               <  1:4988521/93‑1 (MQ=255)
            gCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCGaaaaa                                                                             >  2:4201009/1‑93 (MQ=255)
             ccGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAAt                                                                            >  2:9571892/1‑93 (MQ=255)
                tGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATgtt                                                                         >  1:1615233/1‑91 (MQ=255)
                tGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGt                                                                         >  2:5979883/1‑93 (MQ=255)
                 gCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTc                                                                        <  1:1624315/93‑1 (MQ=255)
                  cGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTcc                                                                       >  1:4161105/1‑93 (MQ=255)
                   gAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCa                                                                      <  1:2976220/93‑1 (MQ=255)
                   gAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCa                                                                      <  2:8738185/93‑1 (MQ=255)
                     aaGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACg                                                                    <  1:1556974/93‑1 (MQ=255)
                         cGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGGGCGGCTTGACCTAAAAATGGCCGACGAACt                                                                >  2:6176496/1‑93 (MQ=255)
                         cGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACt                                                                <  2:7619123/93‑1 (MQ=255)
                         cGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACt                                                                >  1:8575169/1‑93 (MQ=255)
                         cGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACt                                                                >  1:8575167/1‑93 (MQ=255)
                          gCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTg                                                               <  2:9900897/93‑1 (MQ=255)
                           cAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTgg                                                              <  1:2988139/93‑1 (MQ=255)
                             agccgcGTGGACCTGAGCAAAGCAAAGCGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTggcg                                                            <  2:7677912/93‑1 (MQ=255)
                             agcagcGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTggcg                                                            >  1:1978120/1‑93 (MQ=255)
                               cagcGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTggcggc                                                          <  1:4615530/93‑1 (MQ=255)
                               cagcGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTggcggc                                                          <  2:7349872/93‑1 (MQ=255)
                                agcGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGgcggcg                                                         >  1:733911/1‑93 (MQ=255)
                                    tGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgc                                                     <  1:911332/93‑1 (MQ=255)
                                       gccTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctga                                                  <  2:6343683/92‑1 (MQ=255)
                                       aCCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctga                                                  >  1:9056851/1‑93 (MQ=255)
                                        ccTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaa                                                 <  1:2459983/93‑1 (MQ=255)
                                        ccTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaa                                                 <  1:5799029/93‑1 (MQ=255)
                                         cTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaat                                                <  2:9448992/93‑1 (MQ=255)
                                         cTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaat                                                <  1:9866912/93‑1 (MQ=255)
                                         cTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaat                                                >  1:5923480/1‑93 (MQ=255)
                                         cTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaat                                                <  1:3915731/93‑1 (MQ=255)
                                          tGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatg                                               >  2:2509933/1‑93 (MQ=255)
                                          tGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGcgctgaatg                                               >  1:7880058/1‑93 (MQ=255)
                                            agcaaagcaaAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatgct                                             >  2:7168679/1‑93 (MQ=255)
                                              caaagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatgctga                                           <  2:2303799/93‑1 (MQ=255)
                                               aaagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatgctgaa                                          >  1:412778/1‑93 (MQ=255)
                                               aaagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatgctgaa                                          <  1:2712241/93‑1 (MQ=255)
                                               aaagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatgctgaa                                          >  2:9403257/1‑93 (MQ=255)
                                               aaagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatgctgaa                                          >  1:6999946/1‑93 (MQ=255)
                                               aaagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGAGGACCTAAAAATGGTCAACGACCTGGCGGCGGggctgaaggctgag                                          >  1:2317242/1‑92 (MQ=255)
                                                aagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAg                                         >  2:4783353/1‑93 (MQ=255)
                                                aagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAg                                         >  2:1688156/1‑93 (MQ=255)
                                                aagcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAg                                         <  1:9103549/93‑1 (MQ=255)
                                                 agcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGt                                        <  2:10025177/93‑1 (MQ=255)
                                                  gcaaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTc                                       >  1:2387040/1‑93 (MQ=255)
                                                   caaaACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCg                                      >  2:3474096/1‑93 (MQ=255)
                                                    aaaaCGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCCCGAACTGGCGGCGGTGCTGAATGCTGAAGTCgg                                     >  1:9703292/1‑93 (MQ=255)
                                                    aaaaCGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCgg                                     >  2:6535441/1‑93 (MQ=255)
                                                    aaaaCGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCgg                                     >  1:2727506/1‑93 (MQ=255)
                                                        cGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGt                                 >  2:2007584/1‑93 (MQ=255)
                                                        cGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGt                                 <  1:2372425/93‑1 (MQ=255)
                                                         gtgtGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGtt                                >  2:6789166/1‑93 (MQ=255)
                                                         gtgtGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGtt                                <  1:8791006/93‑1 (MQ=255)
                                                           gtgGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATCACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCa                              <  2:10046482/93‑1 (MQ=255)
                                                            tgGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCag                             >  1:723044/1‑92 (MQ=255)
                                                            tgGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCAc                             <  1:2130300/93‑1 (MQ=255)
                                                              gTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGt                           <  1:502013/93‑1 (MQ=255)
                                                               ttGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGGATGCTGAAGTCGGCTGTTGACGTc                          >  1:5514793/1‑93 (MQ=255)
                                                               ttGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTc                          >  1:10005533/1‑93 (MQ=255)
                                                                 ggCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCa                        <  1:5737400/93‑1 (MQ=255)
                                                                   cGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAAt                      <  1:4510089/93‑1 (MQ=255)
                                                                    gtcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTGACGTCCCAtt                     >  2:2831268/1‑93 (MQ=255)
                                                                    gtcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAAtt                     <  1:5979884/93‑1 (MQ=255)
                                                                    gtcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAAtt                     <  2:8916718/93‑1 (MQ=255)
                                                                     tcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCCATTg                    >  1:10021877/1‑93 (MQ=255)
                                                                     tcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAAttc                    >  1:2240927/1‑92 (MQ=255)
                                                                     tcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTg                    >  2:4595496/1‑93 (MQ=255)
                                                                     tcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTg                    <  2:7930015/93‑1 (MQ=255)
                                                                     tcggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTg                    >  1:7666772/1‑93 (MQ=255)
                                                                       ggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGcc                  <  1:8851376/93‑1 (MQ=255)
                                                                       ggtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGcc                  <  2:9043570/93‑1 (MQ=255)
                                                                        gtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCg                 >  1:6676167/1‑93 (MQ=255)
                                                                        gtcgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCg                 >  1:8371172/1‑93 (MQ=255)
                                                                          cgTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCTGCTGTTCACGTCCAATTGCCCaa               >  2:5318215/1‑93 (MQ=255)
                                                                           gTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAg              <  2:5407559/93‑1 (MQ=255)
                                                                           gTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAg              >  1:8097634/1‑93 (MQ=255)
                                                                           gTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAg              >  1:8097635/1‑93 (MQ=255)
                                                                              gACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCg           >  2:7181512/1‑93 (MQ=255)
                                                                               aCTGGCGGCGCGGGATGACCTAACAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCga          <  2:5497260/93‑1 (MQ=255)
                                                                               aCTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCga          <  1:7301598/93‑1 (MQ=255)
                                                                                cTGGCGGCGCGCGATTACCTAAAAAGGGTCCACAAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCgag         <  2:3962228/93‑1 (MQ=255)
                                                                                 tGGCGGCGCGGGATGACCTAAACATGGTCCACGAACTGGCGGCGGAGCTGAATGCTGAAGTCGGCTGTTCACGACCAATTGCAGAAGGCgagt        <  1:8944017/93‑2 (MQ=255)
                                                                                 gggcggcGCGGGATGACCTAAAAATGGTCCACGACCTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCgaga        <  2:8407034/92‑1 (MQ=255)
                                                                                   gcggcgCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAAc      >  2:636169/1‑93 (MQ=255)
                                                                                   gcggcgCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAAc      <  1:9174975/93‑1 (MQ=255)
                                                                                    cggcgCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACt     <  1:34297/93‑1 (MQ=255)
                                                                                     ggcgCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtg    <  1:1264437/93‑1 (MQ=255)
                                                                                     ggcgCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtg    <  1:4566208/93‑1 (MQ=255)
                                                                                      gcgcGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtgg   >  1:2001653/1‑93 (MQ=255)
                                                                                      gcgcGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtgg   >  1:9881113/1‑93 (MQ=255)
                                                                                       cgcgGGATGACCTATAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCCCGTCCAATTGCCGAAGGCGAGAACtgga  <  2:8660700/93‑1 (MQ=255)
                                                                                       cgcgGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtgga  <  2:9512371/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGA  >  NC_000913/1778954‑1779133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGA  >  NC_000913/1778952‑1779137
                                                                                            |                                                                                             
TCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGG                                                                                              <  SRR4302540.4310764/94‑1 (MQ=60)
 CTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGG                                                                                             <  SRR4302540.2349252/94‑1 (MQ=60)
 CTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGG                                                                                             >  SRR4302540.6075171/1‑94 (MQ=60)
  TCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGA                                                                                            >  SRR4302540.8067449/1‑94 (MQ=60)
  TCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGA                                                                                            >  SRR4302540.8827913/1‑94 (MQ=60)
     GTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGA                                                                                         <  SRR4302540.1135888/94‑1 (MQ=60)
      TCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGAC                                                                                        <  SRR4302540.8484304/94‑1 (MQ=60)
       CGCGCACGCCGTGCGGACGCCGCAAGCAGCGTGGTCCTGCGCGCAGCTGACCGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGCTGACC                                                                                       <  SRR4302540.9028222/94‑1 (MQ=25)
       CGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACC                                                                                       <  SRR4302540.1460386/94‑1 (MQ=60)
       CGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACC                                                                                       >  SRR4302540.5758256/1‑94 (MQ=60)
            ACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAA                                                                                  <  SRR4302540.5042530/94‑1 (MQ=60)
             CGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTCAAAA                                                                                 >  SRR4302540.7916383/1‑94 (MQ=60)
                 GTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGTT                                                                             >  SRR4302540.1632953/1‑94 (MQ=60)
                   GCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCC                                                                           <  SRR4302540.4800649/94‑1 (MQ=60)
                   GCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCC                                                                           <  SRR4302540.1642107/94‑1 (MQ=60)
                   GCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCC                                                                           >  SRR4302540.4206205/1‑94 (MQ=60)
                     GAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCAG                                                                         >  SRR4302540.4495346/1‑94 (MQ=60)
                     GAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCAC                                                                         <  SRR4302540.3009112/94‑1 (MQ=60)
                       AAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGA                                                                       <  SRR4302540.1574091/94‑1 (MQ=60)
                          CCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACT                                                                    >  SRR4302540.8674807/1‑94 (MQ=60)
                          CCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACT                                                                    >  SRR4302540.8674805/1‑94 (MQ=60)
                             CAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGC                                                                 >  SRR4302540.1900364/1‑94 (MQ=60)
                             CAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGC                                                                 >  SRR4302540.10056179/1‑94 (MQ=60)
                             CAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGC                                                                 <  SRR4302540.3021094/94‑1 (MQ=60)
                              AAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCG                                                                >  SRR4302540.1999582/1‑94 (MQ=60)
                                 CAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCG                                                             <  SRR4302540.4665041/94‑1 (MQ=60)
                                 CAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCG                                                             >  SRR4302540.741761/1‑94 (MQ=60)
                                   GCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGT                                                           <  SRR4302540.8167325/94‑1 (MQ=60)
                                      TGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCT                                                        <  SRR4302540.7556084/94‑1 (MQ=60)
                                      TGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCT                                                        <  SRR4302540.920976/94‑1 (MQ=60)
                                       GGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTG                                                       >  SRR4302540.2640711/1‑94 (MQ=60)
                                        GACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGA                                                      >  SRR4302540.9165581/1‑94 (MQ=60)
                                         ACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAA                                                     <  SRR4302540.3986710/94‑1 (MQ=60)
                                         ACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAA                                                     <  SRR4302540.7063865/94‑1 (MQ=60)
                                          CCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAAT                                                    <  SRR4302540.2921183/94‑1 (MQ=60)
                                          CCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAAT                                                    <  SRR4302540.2486459/94‑1 (MQ=60)
                                          CCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAAT                                                    <  SRR4302540.5863108/94‑1 (MQ=60)
                                          CCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAAT                                                    >  SRR4302540.5988928/1‑94 (MQ=60)
                                          CCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAAT                                                    >  SRR4302540.7353150/1‑94 (MQ=60)
                                           CTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATG                                                   <  SRR4302540.9982166/94‑1 (MQ=60)
                                           CTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATG                                                   <  SRR4302540.3958133/94‑1 (MQ=60)
                                           CTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGCGCTGAATG                                                   >  SRR4302540.7972082/1‑94 (MQ=60)
                                                CAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAA                                              >  SRR4302540.7077626/1‑94 (MQ=60)
                                                CAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAA                                              >  SRR4302540.417103/1‑94 (MQ=60)
                                                CAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGAGGACCTAAAAATGGTCAACGACCTGGCGGCGGGGCTGAAGGCTGAG                                              >  SRR4302540.2342189/1‑94 (MQ=60)
                                                 AAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAG                                             <  SRR4302540.2741491/94‑1 (MQ=60)
                                                  AAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGT                                            <  SRR4302540.9212942/94‑1 (MQ=60)
                                                   AGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTC                                           >  SRR4302540.2412768/1‑94 (MQ=60)
                                                    GCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCG                                          >  SRR4302540.9213648/1‑94 (MQ=60)
                                                     CAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCCCGAACTGGCGGCGGTGCTGAATGCTGAAGTCGG                                         >  SRR4302540.9817356/1‑94 (MQ=60)
                                                     CAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGG                                         >  SRR4302540.9365286/1‑94 (MQ=60)
                                                     CAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGG                                         >  SRR4302540.2756921/1‑94 (MQ=60)
                                                      AAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGC                                        >  SRR4302540.3571056/1‑94 (MQ=60)
                                                      AAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGC                                        >  SRR4302540.5938720/1‑94 (MQ=60)
                                                       AAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCT                                       <  SRR4302540.2387129/94‑1 (MQ=60)
                                                          CGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTT                                    <  SRR4302540.2397988/94‑1 (MQ=60)
                                                          CGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTT                                    <  SRR4302540.1509672/94‑1 (MQ=60)
                                                          CGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGCCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGgtttc                                    >  SRR4302540.3415785/1‑89 (MQ=60)
                                                           GTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTC                                   <  SRR4302540.8892710/94‑1 (MQ=60)
                                                             GTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCAG                                 >  SRR4302540.730793/1‑94 (MQ=60)
                                                             GTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCAC                                 <  SRR4302540.2097913/94‑1 (MQ=60)
                                                             GTGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCAC                                 <  SRR4302540.9964651/94‑1 (MQ=60)
                                                              TGGTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACG                                <  SRR4302540.2153159/94‑1 (MQ=60)
                                                                GTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGGATGCTGAAGTCGGCTGTTGACGTC                              >  SRR4302540.5575535/1‑94 (MQ=60)
                                                                GTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTC                              >  SRR4302540.8278755/1‑94 (MQ=60)
                                                                GTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTC                              >  SRR4302540.10122069/1‑94 (MQ=60)
                                                                GTTGGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTC                              <  SRR4302540.507399/94‑1 (MQ=60)
                                                                   GGCGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAA                           <  SRR4302540.5800627/94‑1 (MQ=60)
                                                                     CGTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATT                         <  SRR4302540.4558958/94‑1 (MQ=60)
                                                                      GTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCCATTG                        >  SRR4302540.10138504/1‑94 (MQ=60)
                                                                      GTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTG                        <  SRR4302540.6046090/94‑1 (MQ=60)
                                                                      GTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTG                        >  SRR4302540.7753167/1‑94 (MQ=60)
                                                                      GTCGGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTC                        >  SRR4302540.2265011/1‑94 (MQ=60)
                                                                         GGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCG                     >  SRR4302540.6749918/1‑94 (MQ=60)
                                                                         GGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCG                     <  SRR4302540.8953773/94‑1 (MQ=60)
                                                                         GGTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCG                     >  SRR4302540.8468320/1‑94 (MQ=60)
                                                                          GTCGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGA                    <  SRR4302540.215297/94‑1 (MQ=60)
                                                                            CGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAG                  >  SRR4302540.8191627/1‑94 (MQ=60)
                                                                            CGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAG                  >  SRR4302540.8191628/1‑94 (MQ=60)
                                                                            CGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAG                  >  SRR4302540.3186610/1‑94 (MQ=60)
                                                                            CGTGGACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAG                  <  SRR4302540.2461641/94‑1 (MQ=60)
                                                                                 ACTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAG             <  SRR4302540.7383096/94‑1 (MQ=60)
                                                                                  CTGGCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGA            >  SRR4302540.5196220/1‑94 (MQ=60)
                                                                                   TGGCGGCGCGGGATGACCTAAACATGGTCCACGAACTGGCGGCGGAGCTGAATGCTGAAGTCGGCTGTTCACGACCAATTGCAGAAGGCGAGTA           <  SRR4302540.9049139/94‑1 (MQ=60)
                                                                                     GCGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACT         <  SRR4302540.9285028/94‑1 (MQ=60)
                                                                                      CGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTG        >  SRR4302540.1607231/1‑94 (MQ=60)
                                                                                      CGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTG        <  SRR4302540.34661/94‑1 (MQ=60)
                                                                                      CGGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTG        <  SRR4302540.9132481/94‑1 (MQ=60)
                                                                                       GGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGG       >  SRR4302540.2023325/1‑94 (MQ=60)
                                                                                       GGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGG       <  SRR4302540.1277430/94‑1 (MQ=60)
                                                                                       GGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGG       >  SRR4302540.9996492/1‑94 (MQ=60)
                                                                                       GGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGG       <  SRR4302540.4563616/94‑1 (MQ=60)
                                                                                       GGCGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGG       <  SRR4302540.4615513/94‑1 (MQ=60)
                                                                                         CGCGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCCCGTCCAATTGCCGAAGGggaacactggag     >  SRR4302540.373778/1‑82 (MQ=60)
                                                                                           CGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGG   >  SRR4302540.2430863/1‑94 (MQ=60)
                                                                                           CGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGG   >  SRR4302540.272442/1‑94 (MQ=60)
                                                                                           CGGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGG   >  SRR4302540.6216031/1‑94 (MQ=60)
                                                                                            GGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGGACTGGATGGG  >  SRR4302540.5557413/1‑94 (MQ=60)
                                                                                            GGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGA  <  SRR4302540.6979442/94‑1 (MQ=60)
                                                                                            |                                                                                             
TCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGA  >  NC_000913/1778952‑1779137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑