Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,841,661 A→G T423A (ACC→GCC)  gdhA → NADP‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,6610AG99.6% 859.6 / ‑5.0 264T423A (ACC→GCC) gdhANADP‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (157/106);  total (157/107)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 268 total aligned reads displayed.

TGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGA  >  NC_000913/1841571‑1841753
                                                                                          |                                                                                            
agaaaaGCAGAGAAAGTAGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGACTGTGTTGAGCAAGGTGGTGAAGGTGAGCAAGcc                                                                                            <  1:8955196/90‑1 (MQ=255)
 ggAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCa                                                                                           >  1:7261489/1‑93 (MQ=255)
 ggAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCa                                                                                           >  2:9479435/1‑93 (MQ=255)
 ggAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCa                                                                                           >  2:4291650/1‑93 (MQ=255)
  gAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCaa                                                                                          <  1:8188857/93‑1 (MQ=255)
   aaaGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAAc                                                                                         <  2:3878020/93‑1 (MQ=255)
     aGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTa                                                                                       <  2:2151134/93‑1 (MQ=255)
      gCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTAc                                                                                      >  2:7524479/1‑93 (MQ=255)
      accGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTAc                                                                                      <  1:8004534/92‑1 (MQ=255)
       ccGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACg                                                                                     <  2:8658861/93‑1 (MQ=255)
       ccGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACg                                                                                     <  2:2606000/93‑1 (MQ=255)
       ccGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACg                                                                                     <  2:5481538/93‑1 (MQ=255)
        cGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGt                                                                                    <  1:6274355/93‑1 (MQ=255)
         gagaAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTg                                                                                   >  2:6281873/1‑93 (MQ=255)
           gaAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGTTGAAGGTGAGCAAGCCAACTACGTGCa                                                                                 <  2:3162828/93‑1 (MQ=255)
           gaAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCa                                                                                 >  2:9216292/1‑93 (MQ=255)
            aaaGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAg                                                                                <  1:7812891/93‑1 (MQ=255)
             aaGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAgg                                                                               <  2:9372969/93‑1 (MQ=255)
               gTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGgc                                                                             >  1:7406328/1‑93 (MQ=255)
               gTTGACGCACGTTGGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGgc                                                                             <  2:1502312/93‑1 (MQ=255)
                ttGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGgcg                                                                            >  1:4395131/1‑93 (MQ=255)
                ttGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGgcg                                                                            >  1:6350025/1‑93 (MQ=255)
                   aCGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGa                                                                         >  1:5172146/1‑93 (MQ=255)
                   aCGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGa                                                                         <  2:4137107/93‑1 (MQ=255)
                    cGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGaa                                                                        >  2:4646015/1‑93 (MQ=255)
                    cGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGaa                                                                        <  1:2733947/93‑1 (MQ=255)
                     gCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAAc                                                                       <  1:1889326/93‑1 (MQ=255)
                      cACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACa                                                                      >  1:612484/1‑93 (MQ=255)
                      cACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACa                                                                      <  1:3366443/93‑1 (MQ=255)
                      cACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACa                                                                      <  1:6937745/93‑1 (MQ=255)
                       aCGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACAt                                                                     <  1:5391551/93‑1 (MQ=255)
                        cGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACAtt                                                                    >  2:603045/1‑93 (MQ=255)
                          tttGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGc                                                                  >  2:1654737/1‑93 (MQ=255)
                          tttGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGc                                                                  >  1:6835830/1‑93 (MQ=255)
                          tttGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGc                                                                  >  1:2087525/1‑93 (MQ=255)
                             gCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCgg                                                               >  1:2476476/1‑93 (MQ=255)
                               atcacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGtt                                                             >  1:9320155/1‑93 (MQ=255)
                               atcacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGtt                                                             <  2:4014251/93‑1 (MQ=255)
                                tcacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGttt                                                            >  1:996001/1‑93 (MQ=255)
                                 cacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGtttt                                                           <  2:8287831/93‑1 (MQ=255)
                                 cacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGtttt                                                           >  2:1449522/1‑93 (MQ=255)
                                 cacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGgttt                                                           >  1:4186178/1‑93 (MQ=255)
                                    atcatGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTtgtg                                                        >  1:9016891/1‑93 (MQ=255)
                                      catGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGaa                                                      <  2:2346115/93‑1 (MQ=255)
                                      catGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGaa                                                      <  1:8357224/93‑1 (MQ=255)
                                      catGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGaa                                                      <  2:7172836/93‑1 (MQ=255)
                                       atGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAg                                                     >  1:9226187/1‑93 (MQ=255)
                                         gCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGt                                                   <  1:5778449/93‑1 (MQ=255)
                                         gCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGt                                                   >  2:9633806/1‑93 (MQ=255)
                                           tGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGCAgtgtg                                                 >  1:5992375/1‑89 (MQ=255)
                                           tGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTg                                                 >  1:10012227/1‑93 (MQ=255)
                                           tGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGGTAAGGTTg                                                 >  1:9273521/1‑93 (MQ=255)
                                               tatCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcga                                             >  1:3072913/1‑93 (MQ=255)
                                               tatCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcga                                             <  1:6611576/93‑1 (MQ=255)
                                               tatCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcga                                             >  2:1781556/1‑93 (MQ=255)
                                               tatCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcga                                             >  1:7911712/1‑93 (MQ=255)
                                                 tCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatg                                           >  2:2039241/1‑93 (MQ=255)
                                                  ccaccaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgc                                          <  2:6595963/93‑1 (MQ=255)
                                                  ccaccaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgc                                          <  1:9403752/93‑1 (MQ=255)
                                                  ccaccaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgc                                          >  1:3729397/1‑93 (MQ=255)
                                                   caccaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcg                                         >  1:7542174/1‑93 (MQ=255)
                                                    accaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcga                                        >  1:5638458/1‑93 (MQ=255)
                                                    accaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcga                                        >  2:7906206/1‑93 (MQ=255)
                                                     ccaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcgat                                       >  2:2472197/1‑93 (MQ=255)
                                                     ccaTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcgat                                       <  1:3570436/93‑1 (MQ=255)
                                                      caTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcgatg                                      <  1:5038293/93‑1 (MQ=255)
                                                       aTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCgatgcgatgc                                     >  1:8085835/1‑93 (MQ=255)
                                                       aTGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCgatgcgatgc                                     >  2:7243050/1‑93 (MQ=255)
                                                        tGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCt                                    <  1:1999278/93‑1 (MQ=255)
                                                        tGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCt                                    <  2:5037858/93‑1 (MQ=255)
                                                          ccTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTgg                                  <  1:6478368/93‑1 (MQ=255)
                                                           cTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGgc                                 >  2:3270000/1‑93 (MQ=255)
                                                            tgtgTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGgcg                                >  2:3530161/1‑93 (MQ=255)
                                                             gtgtTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGgcgc                               >  1:4558991/1‑93 (MQ=255)
                                                               gtTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAg                             >  2:2688977/1‑93 (MQ=255)
                                                               gtTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAg                             <  1:9855645/93‑1 (MQ=255)
                                                               gtTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAg                             <  2:6178176/93‑1 (MQ=255)
                                                                 tGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAggg                           >  1:5915612/1‑93 (MQ=255)
                                                                 tGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAggg                           <  2:445788/93‑1 (MQ=255)
                                                                  gAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGgt                          <  1:5660071/93‑1 (MQ=255)
                                                                    gCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGgtgt                        >  2:4838179/1‑93 (MQ=255)
                                                                       tggtggAGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGATTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGCGTGAt                     >  1:2851585/1‑93 (MQ=255)
                                                                        ggtggtGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAtt                    <  2:8918323/93‑1 (MQ=255)
                                                                        ggtggtGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAtt                    <  2:3654516/93‑1 (MQ=255)
                                                                         gtggtgAAGGTGAGCAAGCCAACTACGTGCAGGGCACGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAttt                   >  2:1058717/1‑93 (MQ=255)
                                                                          tggtgAAGGTGAGCAAGCCCACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTa                  >  2:1230470/1‑93 (MQ=255)
                                                                          tggtgAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTa                  <  2:4547790/93‑1 (MQ=255)
                                                                             tgAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGt               <  2:5274973/93‑1 (MQ=255)
                                                                               aaGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTg             >  2:5099156/1‑93 (MQ=255)
                                                                                 ggTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTa           <  2:5749944/93‑1 (MQ=255)
                                                                                 ggTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTa           >  2:1576589/1‑93 (MQ=255)
                                                                                  gTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTaa          <  1:2570478/93‑1 (MQ=255)
                                                                                  gTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTaa          <  1:3953586/93‑1 (MQ=255)
                                                                                   tGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTaaa         <  2:9819540/93‑1 (MQ=255)
                                                                                    gAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAAt        <  1:9729249/93‑1 (MQ=255)
                                                                                    gAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAAt        <  1:2289086/93‑1 (MQ=255)
                                                                                     aGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAAtt       >  2:7757455/1‑92 (MQ=255)
                                                                                     aGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATg       <  2:2916888/93‑1 (MQ=255)
                                                                                     aGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATg       <  1:7110516/93‑1 (MQ=255)
                                                                                      gCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGc      >  1:1452431/1‑93 (MQ=255)
                                                                                          |                                                                                            
TGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGA  >  NC_000913/1841571‑1841753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGAT  >  NC_000913/1841568‑1841754
                                                                                             |                                                                                             
GGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAG                                                                                               <  SRR4302540.1008833/94‑1 (MQ=60)
GGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAG                                                                                               >  SRR4302540.2133146/1‑94 (MQ=60)
  CTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCC                                                                                             >  SRR4302540.5654695/1‑94 (MQ=60)
  CTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCC                                                                                             <  SRR4302540.6230210/94‑1 (MQ=60)
   agaAAAGCAGAGAAAGTAGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGACTGTGTTGAGCAAGGTGGTGAAGGTGAGCAAGCCA                                                                                            <  SRR4302540.9060931/91‑1 (MQ=60)
   TGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCA                                                                                            >  SRR4302540.7342510/1‑94 (MQ=60)
   TGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCA                                                                                            >  SRR4302540.9345615/1‑94 (MQ=60)
     GAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAAC                                                                                          <  SRR4302540.8283996/94‑1 (MQ=60)
      AAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACT                                                                                         >  SRR4302540.9267869/1‑94 (MQ=60)
      AAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACT                                                                                         >  SRR4302540.9103288/1‑94 (MQ=60)
      AAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACT                                                                                         >  SRR4302540.781946/1‑94 (MQ=60)
         ACCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACG                                                                                      <  SRR4302540.8097686/94‑1 (MQ=60)
           CGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTG                                                                                    <  SRR4302540.6344160/94‑1 (MQ=60)
           CGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTG                                                                                    >  SRR4302540.3662880/1‑94 (MQ=60)
            GAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGC                                                                                   >  SRR4302540.664985/1‑94 (MQ=60)
            GAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGC                                                                                   <  SRR4302540.2511871/94‑1 (MQ=60)
             AGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCA                                                                                  >  SRR4302540.6152719/1‑94 (MQ=60)
             AGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCA                                                                                  >  SRR4302540.5900869/1‑94 (MQ=60)
             AGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCA                                                                                  <  SRR4302540.9850910/94‑1 (MQ=60)
              GAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAG                                                                                 <  SRR4302540.523903/94‑1 (MQ=60)
              GAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAG                                                                                 <  SRR4302540.9518313/94‑1 (MQ=60)
               AAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGG                                                                                >  SRR4302540.622227/1‑94 (MQ=60)
               AAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGG                                                                                <  SRR4302540.7904191/94‑1 (MQ=60)
                AAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGG                                                                               <  SRR4302540.7755205/94‑1 (MQ=60)
                 AGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGC                                                                              >  SRR4302540.7488835/1‑94 (MQ=60)
                  GTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCG                                                                             >  SRR4302540.4442817/1‑94 (MQ=60)
                  GTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCG                                                                             >  SRR4302540.6420741/1‑94 (MQ=60)
                  GTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCG                                                                             >  SRR4302540.6420742/1‑94 (MQ=60)
                    TGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCG                                                                           >  SRR4302540.6798398/1‑94 (MQ=60)
                     GACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGA                                                                          >  SRR4302540.5228433/1‑94 (MQ=60)
                     GACGCACGTTTGCATCACATAATGCTGGATATCCACCATGCCTGTGTTGCGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGA                                                                          >  SRR4302540.5228432/1‑94 (MQ=60)
                      ACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAA                                                                         <  SRR4302540.3953648/94‑1 (MQ=60)
                       CGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAAC                                                                        >  SRR4302540.7152345/1‑94 (MQ=60)
                       CGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAAC                                                                        <  SRR4302540.2763454/94‑1 (MQ=60)
                        GCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACA                                                                       <  SRR4302540.7404176/94‑1 (MQ=60)
                        GCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACA                                                                       >  SRR4302540.619023/1‑94 (MQ=60)
                        GCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACA                                                                       >  SRR4302540.9007856/1‑94 (MQ=60)
                        GCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACA                                                                       <  SRR4302540.1910007/94‑1 (MQ=60)
                         CACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACAT                                                                      <  SRR4302540.7014967/94‑1 (MQ=60)
                         CACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACAT                                                                      <  SRR4302540.3403267/94‑1 (MQ=60)
                         CACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACAT                                                                      >  SRR4302540.9451122/1‑94 (MQ=60)
                          ACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATT                                                                     <  SRR4302540.5450840/94‑1 (MQ=60)
                            GTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCGTGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGC                                                                   >  SRR4302540.6911541/1‑94 (MQ=60)
                            GTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGC                                                                   >  SRR4302540.6911540/1‑94 (MQ=60)
                            GTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGC                                                                   >  SRR4302540.2109958/1‑94 (MQ=60)
                            GTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGC                                                                   >  SRR4302540.806731/1‑94 (MQ=60)
                            GTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGC                                                                   >  SRR4302540.7192693/1‑94 (MQ=60)
                              TTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCG                                                                 <  SRR4302540.8605076/94‑1 (MQ=60)
                              TTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCG                                                                 >  SRR4302540.9986491/1‑94 (MQ=60)
                              TTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCG                                                                 >  SRR4302540.3478318/1‑94 (MQ=60)
                               TGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGG                                                                >  SRR4302540.2503141/1‑94 (MQ=60)
                                 CATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTT                                                              >  SRR4302540.9431359/1‑94 (MQ=60)
                                  ATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTT                                                             >  SRR4302540.1006141/1‑94 (MQ=60)
                                  ATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTT                                                             >  SRR4302540.2698191/1‑94 (MQ=60)
                                   TCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTT                                                            >  SRR4302540.7259461/1‑94 (MQ=60)
                                   TCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTT                                                            >  SRR4302540.4231680/1‑94 (MQ=60)
                                   TCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGGTTT                                                            >  SRR4302540.4231679/1‑94 (MQ=60)
                                     ACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGT                                                          >  SRR4302540.2431592/1‑94 (MQ=60)
                                      CATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTG                                                         >  SRR4302540.9125088/1‑94 (MQ=60)
                                       ATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGA                                                        >  SRR4302540.5989821/1‑94 (MQ=60)
                                       ATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGA                                                        >  SRR4302540.5989822/1‑94 (MQ=60)
                                         CATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAG                                                      <  SRR4302540.8287352/94‑1 (MQ=60)
                                         CATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAG                                                      <  SRR4302540.8454209/94‑1 (MQ=60)
                                         CATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAG                                                      >  SRR4302540.9336807/1‑94 (MQ=60)
                                         CATGCGGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAG                                                      <  SRR4302540.7563689/94‑1 (MQ=60)
                                          ATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGG                                                     <  SRR4302540.7980452/94‑1 (MQ=60)
                                          ATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGG                                                     >  SRR4302540.7109704/1‑94 (MQ=60)
                                          ATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGG                                                     >  SRR4302540.1393730/1‑94 (MQ=60)
                                          ATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGG                                                     <  SRR4302540.459920/94‑1 (MQ=60)
                                           TGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGT                                                    <  SRR4302540.5788995/94‑1 (MQ=60)
                                            GCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTT                                                   <  SRR4302540.5842282/94‑1 (MQ=60)
                                            GCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTT                                                   >  SRR4302540.5726804/1‑94 (MQ=60)
                                             ttgtgTATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTG                                                  <  SRR4302540.3404397/89‑1 (MQ=60)
                                             CTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGcagtgtg                                                  >  SRR4302540.6058762/1‑87 (MQ=60)
                                             CTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTG                                                  >  SRR4302540.10128803/1‑94 (MQ=60)
                                             CTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTG                                                  <  SRR4302540.3404396/94‑1 (MQ=60)
                                             CTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGGTAAGGTTG                                                  >  SRR4302540.9384400/1‑94 (MQ=60)
                                                 ATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGA                                              >  SRR4302540.3106627/1‑94 (MQ=60)
                                                 ATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGA                                              >  SRR4302540.8003959/1‑94 (MQ=60)
                                                  TATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGAT                                             <  SRR4302540.6743360/94‑1 (MQ=60)
                                                  TATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGAT                                             <  SRR4302540.6684785/94‑1 (MQ=60)
                                                  TATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGAT                                             >  SRR4302540.9648885/1‑94 (MQ=60)
                                                  TATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGAT                                             <  SRR4302540.1492461/94‑1 (MQ=60)
                                                   ATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATG                                            >  SRR4302540.272495/1‑94 (MQ=60)
                                                    TCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGC                                           >  SRR4302540.3770080/1‑94 (MQ=60)
                                                    TCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGC                                           <  SRR4302540.1765650/94‑1 (MQ=60)
                                                     CCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCG                                          <  SRR4302540.9515609/94‑1 (MQ=60)
                                                     CCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCG                                          >  SRR4302540.7626672/1‑94 (MQ=60)
                                                     CCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCG                                          >  SRR4302540.7626673/1‑94 (MQ=60)
                                                     CCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCG                                          >  SRR4302540.591471/1‑94 (MQ=60)
                                                      CACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGA                                         >  SRR4302540.4018511/1‑94 (MQ=60)
                                                      CACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGA                                         >  SRR4302540.5700516/1‑94 (MQ=60)
                                                       CCCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        <  SRR4302540.2014891/94‑1 (MQ=60)
                                                       ACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        >  SRR4302540.941735/1‑94 (MQ=60)
                                                       ACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        <  SRR4302540.8120842/94‑1 (MQ=60)
                                                       ACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        <  SRR4302540.4774332/94‑1 (MQ=60)
                                                       ACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        <  SRR4302540.2820400/94‑1 (MQ=60)
                                                       ACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        >  SRR4302540.813908/1‑94 (MQ=60)
                                                       ACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGAT                                        <  SRR4302540.9371451/94‑1 (MQ=60)
                                                        CCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATG                                       <  SRR4302540.3609436/94‑1 (MQ=60)
                                                         CATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGC                                      >  SRR4302540.8179684/1‑94 (MQ=60)
                                                         CATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGC                                      <  SRR4302540.5092791/94‑1 (MQ=60)
                                                          ATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCT                                     >  SRR4302540.9472221/1‑94 (MQ=60)
                                                          ATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCT                                     >  SRR4302540.2443602/1‑94 (MQ=60)
                                                           TGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTG                                    >  SRR4302540.807909/1‑94 (MQ=60)
                                                           TGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTG                                    <  SRR4302540.2020932/94‑1 (MQ=60)
                                                           TGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTG                                    >  SRR4302540.2894383/1‑94 (MQ=60)
                                                           TGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTG                                    >  SRR4302540.983927/1‑94 (MQ=60)
                                                           TGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTG                                    >  SRR4302540.6417511/1‑94 (MQ=60)
                                                            GCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGG                                   <  SRR4302540.5540456/94‑1 (MQ=60)
                                                            GCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGG                                   <  SRR4302540.620043/94‑1 (MQ=60)
                                                             CCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGC                                  <  SRR4302540.2307515/94‑1 (MQ=60)
                                                             CCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGC                                  <  SRR4302540.6550438/94‑1 (MQ=60)
                                                             CCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGC                                  <  SRR4302540.3270150/94‑1 (MQ=60)
                                                               TGTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGC                                >  SRR4302540.4608261/1‑94 (MQ=60)
                                                                GTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCG                               >  SRR4302540.6617673/1‑94 (MQ=60)
                                                                GTGTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCA                               >  SRR4302540.5257347/1‑94 (MQ=60)
                                                                  GTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGG                             <  SRR4302540.9970826/94‑1 (MQ=60)
                                                                  GTTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGG                             >  SRR4302540.4211199/1‑94 (MQ=60)
                                                                   TTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGG                            <  SRR4302540.5706286/94‑1 (MQ=60)
                                                                   TTGAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGG                            >  SRR4302540.5980962/1‑94 (MQ=60)
                                                                     GAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTG                          <  SRR4302540.5722392/94‑1 (MQ=60)
                                                                     GAGCATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTG                          <  SRR4302540.3837338/94‑1 (MQ=60)
                                                                         ATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAT                      >  SRR4302540.8431818/1‑94 (MQ=60)
                                                                         ATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAT                      >  SRR4302540.2882475/1‑94 (MQ=60)
                                                                         ATGGTGGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGGTTTGTGAAGGATGCCGATGCGATGCTGGCGCAGGGTGTGAT                      >  SRR4302540.5031897/1‑94 (MQ=60)
                                                                         ATGGTGGAGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGATTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGCGTGAT                      >  SRR4302540.2882477/1‑94 (MQ=60)
                                                                              GGTGAAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAG                 >  SRR4302540.203523/1‑94 (MQ=60)
                                                                                  AAGGTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGT             <  SRR4302540.4535860/94‑1 (MQ=60)
                                                                                     GTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAA          >  SRR4302540.1955609/1‑94 (MQ=60)
                                                                                     GTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAA          <  SRR4302540.2598195/94‑1 (MQ=60)
                                                                                     GTGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAA          <  SRR4302540.3996255/94‑1 (MQ=60)
                                                                                      TGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAAT         <  SRR4302540.10108093/94‑1 (MQ=60)
                                                                                      TGAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAAT         <  SRR4302540.3255669/94‑1 (MQ=60)
                                                                                       GAGCAAGCCATCTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATG        <  SRR4302540.9843503/94‑1 (MQ=60)
                                                                                       GAGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATG        <  SRR4302540.2313710/94‑1 (MQ=60)
                                                                                        AGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGC       >  SRR4302540.1468228/1‑94 (MQ=60)
                                                                                        AGCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGC       <  SRR4302540.7189655/94‑1 (MQ=60)
                                                                                         GCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCC      <  SRR4302540.9326493/94‑1 (MQ=60)
                                                                                         GCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCC      >  SRR4302540.647100/1‑94 (MQ=60)
                                                                                         GCAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCC      <  SRR4302540.823878/94‑1 (MQ=60)
                                                                                          CAAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCT     >  SRR4302540.3816654/1‑94 (MQ=60)
                                                                                           AAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTG    >  SRR4302540.8564957/1‑94 (MQ=60)
                                                                                           AAGCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTG    >  SRR4302540.8564958/1‑94 (MQ=60)
                                                                                             GCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTNTGAAGGTTGCCNATGCGATGCTGGCGCAGGNGGNGATTTAAGTTGTAAANNCNTGAT  >  SRR4302540.4630108/1‑94 (MQ=60)
                                                                                             GCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGAT  <  SRR4302540.9422171/94‑1 (MQ=60)
                                                                                             GCCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGAT  <  SRR4302540.6960670/94‑1 (MQ=60)
                                                                                             ACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGAT  <  SRR4302540.2183681/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGAT  >  NC_000913/1841568‑1841754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑