Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,980,940 +C coding (1/363 nt)
coding (627/627 nt)		 araH ←
araH ←		 ABC‑type arabinose transport system permease component
ABC‑type arabinose transport system permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,980,9391.C99.6% 780.7 / ‑4.4 246coding (2/363 nt)araHABC‑type arabinose transport system permease component
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (126/119);  total (126/120)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 275 total aligned reads displayed.

CCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑CTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG  >  NC_000913/1980847‑1981024
                                                                                              |                                                                                     
 cAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑c                                                                                      <  1:9495756/93‑1 (MQ=255)
 cAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑c                                                                                      <  2:3098346/93‑1 (MQ=255)
  aGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cc                                                                                     >  2:5656056/1‑92 (MQ=255)
  aGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cc                                                                                     >  2:7660330/1‑92 (MQ=255)
   gAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cct                                                                                    <  2:4009561/93‑3 (MQ=255)
   gAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cct                                                                                    >  1:1831286/1‑91 (MQ=255)
   gAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cct                                                                                    <  1:8269505/93‑3 (MQ=255)
    aataatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cctc                                                                                   >  1:4420418/1‑90 (MQ=255)
     ataatTCCGGCAATCGCTGATACAAGGCCTGAGAGAACAAAAATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑cctct                                                                                  <  2:9244334/93‑5 (MQ=255)
       aatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTc                                                                                 <  2:2149338/93‑1 (MQ=255)
           ccGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTc                                                                             >  1:394441/1‑93 (MQ=255)
            cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTcc                                                                            <  1:8082591/93‑1 (MQ=255)
            cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTcc                                                                            >  2:1256853/1‑93 (MQ=255)
             ggCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTccc                                                                           >  1:8830909/1‑93 (MQ=255)
              gCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCg                                                                          >  2:3237009/1‑93 (MQ=255)
               cTATCCCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGc                                                                         <  1:9091714/93‑1 (MQ=255)
                tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGcc                                                                        >  2:839530/1‑93 (MQ=255)
                tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGcc                                                                        <  2:8734600/93‑1 (MQ=255)
                  tCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCaa                                                                      <  1:5089634/93‑1 (MQ=255)
                   cGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAAt                                                                     <  1:10020609/93‑1 (MQ=255)
                     cTGATACCAGGCCTGAGAGAACAAAGATAATAATTGTGGTGCGAACAACCGGTACACCCGCCCGACGCGGCGG‑CTCTTCGTTCCCGCCGATCg                                                                   >  2:5162163/1‑93 (MQ=255)
                       gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGcc                                                                 <  1:4508198/93‑1 (MQ=255)
                        aTACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCa                                                                >  2:5400519/1‑93 (MQ=255)
                         tcccAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAg                                                               <  2:5011860/91‑1 (MQ=255)
                         tACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAg                                                               <  1:5258584/93‑1 (MQ=255)
                         tACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAg                                                               <  1:1945356/93‑1 (MQ=255)
                          aCCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAgg                                                              <  2:2533655/93‑1 (MQ=255)
                           ccAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAggg                                                             <  2:1746092/93‑1 (MQ=255)
                            cAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGgt                                                            <  1:1379913/93‑1 (MQ=255)
                              ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGgtgt                                                          <  2:5918677/93‑1 (MQ=255)
                               gCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                         <  1:2837694/93‑1 (MQ=255)
                               gCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                         >  1:685076/1‑93 (MQ=255)
                               gCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                         >  2:6326455/1‑93 (MQ=255)
                                ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                        >  1:4949432/1‑93 (MQ=255)
                                ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                        >  2:9078882/1‑93 (MQ=255)
                                ccTGAAAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                        <  2:2510910/93‑1 (MQ=255)
                                 cTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                       <  1:3662674/93‑1 (MQ=255)
                                  tGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACg                                                      >  1:4223668/1‑93 (MQ=255)
                                   gagagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGa                                                     >  1:4598112/1‑93 (MQ=255)
                                   gagagaACAAAGATAATAATTTGGGTGCAAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGa                                                     <  2:4663002/93‑1 (MQ=255)
                                    agagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAc                                                    >  1:8342189/1‑93 (MQ=255)
                                        aaCTAAGAAAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGCCGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaaa                                                <  1:1305225/93‑1 (MQ=255)
                                        aaCAAAGATAATAATTTTGGTGCGAACAGCCGGCACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaaa                                                <  1:5059694/93‑1 (MQ=255)
                                        aaCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaag                                                >  1:3112485/1‑92 (MQ=255)
                                        aaCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaaa                                                <  2:1039677/93‑1 (MQ=255)
                                         aCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAg                                               <  2:4427195/93‑1 (MQ=255)
                                          cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                              >  2:3815267/1‑93 (MQ=255)
                                          cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                              <  2:8125090/93‑1 (MQ=255)
                                          cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                              >  1:5700935/1‑93 (MQ=255)
                                          cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                              <  2:5980871/93‑1 (MQ=255)
                                          cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                              >  1:6368485/1‑93 (MQ=255)
                                          aaaaGAAAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                              <  1:8503053/92‑1 (MQ=255)
                                           aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggt                                             <  2:3014256/93‑1 (MQ=255)
                                            aaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtg                                            <  2:7476480/93‑1 (MQ=255)
                                            aaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtg                                            <  1:7877759/93‑1 (MQ=255)
                                            aaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggcg                                            >  2:9397606/1‑91 (MQ=255)
                                              gATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                          >  2:8538314/1‑93 (MQ=255)
                                              aataataATTTTGGTGCGACCAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                          <  1:9781110/92‑1 (MQ=255)
                                                taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGAGGttt                                        >  2:9462373/1‑93 (MQ=255)
                                                taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGGGTTACGACCAAAGGTGGttt                                        >  2:2757047/1‑93 (MQ=255)
                                                taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGCGTTACGACCAAAGGTGGttt                                        >  1:545330/1‑93 (MQ=255)
                                                  ataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                      <  2:1699289/93‑1 (MQ=255)
                                                  ataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                      <  1:9003000/93‑1 (MQ=255)
                                                    aatTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAtt                                    >  2:6187981/1‑93 (MQ=255)
                                                     atTTTGGTGCGAACAACCGGTACCCCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTc                                   <  2:8314050/93‑1 (MQ=255)
                                                     atTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTc                                   <  1:1671274/93‑1 (MQ=255)
                                                      ttttGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                  >  1:5817691/1‑93 (MQ=255)
                                                      ttttGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                  >  2:3559447/1‑93 (MQ=255)
                                                      ttttGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGAGGTTTTATTca                                  >  1:4123369/1‑93 (MQ=255)
                                                        ttGGTGCGAACAACGGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagc                                <  1:3360572/93‑1 (MQ=255)
                                                         tGGTGCGAACAACCGGTCCCCCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAACGGTGGTTTTATTcaaca                               <  2:8963119/93‑1 (MQ=255)
                                                         tGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                               >  2:3615496/1‑93 (MQ=255)
                                                         tGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                               <  1:7268267/93‑1 (MQ=255)
                                                         tGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                               <  1:9583329/93‑1 (MQ=255)
                                                          ggTGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagcag                              >  1:2115960/1‑93 (MQ=255)
                                                            tGCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAgcagca                            <  1:4538451/93‑1 (MQ=255)
                                                             gCGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCaa                           <  2:5391278/93‑1 (MQ=255)
                                                              cGAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCaaa                          <  1:6565104/93‑1 (MQ=255)
                                                               gAACAACCGGTACACCCGCCCGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCACAGGTGGTTTTATTCATCAGCaacc                         >  1:6068845/1‑91 (MQ=255)
                                                               gAACAACCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAc                         >  2:4461888/1‑93 (MQ=255)
                                                                 acaacCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                       >  1:7486465/1‑93 (MQ=255)
                                                                 acaacCGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                       >  1:293616/1‑93 (MQ=255)
                                                                  cacccGGTCCACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaa                      <  2:3901148/90‑1 (MQ=255)
                                                                     ccGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGa                   <  2:6827798/93‑1 (MQ=255)
                                                                      cGGTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGAt                  <  2:2011401/93‑1 (MQ=255)
                                                                       ggTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                 >  2:9735910/1‑93 (MQ=255)
                                                                       ggTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                 <  1:4761138/93‑1 (MQ=255)
                                                                        gTACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                >  2:9612994/1‑93 (MQ=255)
                                                                         tACACCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAAt               <  2:4227133/93‑1 (MQ=255)
                                                                           cacCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa             >  2:1566810/1‑93 (MQ=255)
                                                                            acCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATcca            >  2:7157057/1‑91 (MQ=255)
                                                                            acCCGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCACCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAg            >  1:9180068/1‑93 (MQ=255)
                                                                             cccGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGa           >  1:2084921/1‑93 (MQ=255)
                                                                              ccGCCAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGac          <  1:541653/93‑1 (MQ=255)
                                                                               cGCCAGCCGC‑ACGTCCTCTTCGTGCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGacc         <  2:6511314/93‑2 (MQ=255)
                                                                                 ccAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcg       <  2:4709793/93‑1 (MQ=255)
                                                                                  cAGACGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgc      >  2:2847693/1‑93 (MQ=255)
                                                                                     aCGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAc   <  1:9257336/93‑1 (MQ=255)
                                                                                     aCGC‑GCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAc   <  1:4993529/93‑1 (MQ=255)
                                                                                      cgc‑gcgGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACg  >  2:3454389/1‑93 (MQ=255)
                                                                                              |                                                                                     
CCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGC‑GCGG‑CTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG  >  NC_000913/1980847‑1981024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980848‑1981033
                                                                                             |                                                                                              
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑C                                                                                              <  SRR4302540.9608295/94‑1 (MQ=60)
 AGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑Cct                                                                                              >  SRR4302540.1851320/1‑92 (MQ=60)
  GAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑Cctc                                                                                             >  SRR4302540.4468313/1‑91 (MQ=60)
  GAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑Cctc                                                                                             <  SRR4302540.8365591/94‑4 (MQ=60)
     TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTC                                                                                          <  SRR4302540.1026439/94‑1 (MQ=60)
     TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTC                                                                                          <  SRR4302540.2392593/94‑1 (MQ=60)
     TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTC                                                                                          <  SRR4302540.7850761/94‑1 (MQ=60)
      AATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCG                                                                                         >  SRR4302540.7088543/1‑94 (MQ=60)
      AATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCG                                                                                         >  SRR4302540.9672035/1‑94 (MQ=60)
       ATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGT                                                                                        >  SRR4302540.2122788/1‑94 (MQ=60)
        TTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTT                                                                                       >  SRR4302540.8251351/1‑94 (MQ=60)
        TTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTT                                                                                       >  SRR4302540.8430509/1‑94 (MQ=60)
        TTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTT                                                                                       <  SRR4302540.2134061/94‑1 (MQ=60)
         TCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTC                                                                                      >  SRR4302540.398525/1‑94 (MQ=60)
           CGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCC                                                                                    <  SRR4302540.8176400/94‑1 (MQ=60)
           CGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCC                                                                                    <  SRR4302540.5593019/94‑1 (MQ=60)
           CGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCC                                                                                    >  SRR4302540.8933042/1‑94 (MQ=60)
              CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCC                                                                                 >  SRR4302540.411209/1‑94 (MQ=60)
              CTATCCCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCC                                                                                 <  SRR4302540.9200942/94‑1 (MQ=60)
                ATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAA                                                                               <  SRR4302540.5254713/94‑1 (MQ=60)
                 TCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAAT                                                                              <  SRR4302540.5144667/94‑1 (MQ=60)
                 TCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAAT                                                                              <  SRR4302540.5144668/94‑1 (MQ=60)
                  CGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATC                                                                             <  SRR4302540.5039383/94‑1 (MQ=60)
                  CGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATC                                                                             <  SRR4302540.10137229/94‑1 (MQ=60)
                   GCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCG                                                                            >  SRR4302540.7404137/1‑94 (MQ=60)
                     GGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCC                                                                          <  SRR4302540.1970577/94‑1 (MQ=60)
                      GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCG                                                                         >  SRR4302540.6663293/1‑94 (MQ=60)
                      GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCA                                                                         <  SRR4302540.4557050/94‑1 (MQ=60)
                      GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCA                                                                         >  SRR4302540.6392023/1‑94 (MQ=60)
                      GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCA                                                                         >  SRR4302540.172478/1‑94 (MQ=60)
                       ATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAG                                                                        >  SRR4302540.558617/1‑94 (MQ=60)
                       ATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAG                                                                        >  SRR4302540.7677470/1‑94 (MQ=60)
                        TACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGG                                                                       <  SRR4302540.5315959/94‑1 (MQ=60)
                        TACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGG                                                                       <  SRR4302540.1966537/94‑1 (MQ=60)
                           CAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACACCCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTG                                                                    <  SRR4302540.4843013/94‑1 (MQ=60)
                           CAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTG                                                                    <  SRR4302540.1394625/94‑1 (MQ=60)
                           AAGGCCTGAGAGAACAAAGATAATAATTTGGGTGCCAACACCCGGTACGCCCGCCAGACGCGCGGCC‑TCTCCGTTCCCGCCAATCGCCAGGGTG                                                                    <  SRR4302540.7324647/94‑1 (MQ=60)
                            AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   >  SRR4302540.4411756/1‑94 (MQ=60)
                             GGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTT                                                                  >  SRR4302540.692428/1‑94 (MQ=60)
                             GGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTT                                                                  >  SRR4302540.9527223/1‑94 (MQ=60)
                              GCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAGCCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTA                                                                 <  SRR4302540.1812544/94‑1 (MQ=60)
                              GCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTA                                                                 >  SRR4302540.5002895/1‑94 (MQ=60)
                              GCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTA                                                                 <  SRR4302540.2868390/94‑1 (MQ=60)
                              GCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTA                                                                 <  SRR4302540.3227166/94‑1 (MQ=60)
                               CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                >  SRR4302540.8393363/1‑94 (MQ=60)
                               CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                <  SRR4302540.9148779/94‑1 (MQ=60)
                                CTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCGATCGCCAGGGTGTTACG                                                               >  SRR4302540.406175/1‑94 (MQ=60)
                                CTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACG                                                               >  SRR4302540.4269643/1‑94 (MQ=60)
                                CTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACG                                                               <  SRR4302540.3702762/94‑1 (MQ=60)
                                 TGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGA                                                              >  SRR4302540.4647549/1‑94 (MQ=60)
                                 TGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGA                                                              >  SRR4302540.4647548/1‑94 (MQ=60)
                                  GAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAC                                                             >  SRR4302540.8439013/1‑94 (MQ=60)
                                     AGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAA                                                          >  SRR4302540.2001020/1‑94 (MQ=60)
                                      GAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAG                                                         >  SRR4302540.3146524/1‑94 (MQ=60)
                                       AACTAAGAAAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGCCGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        <  SRR4302540.1318764/94‑1 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCGAACAGCCGGCACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        <  SRR4302540.5114400/94‑1 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        >  SRR4302540.9260328/1‑94 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        >  SRR4302540.5035224/1‑94 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        >  SRR4302540.4244316/1‑94 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGATGTTACGACCAAAG                                                        <  SRR4302540.1318765/94‑1 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCAAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        <  SRR4302540.2178694/94‑1 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       <  SRR4302540.7422514/94‑1 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       <  SRR4302540.5125872/94‑1 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302540.6439400/1‑94 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302540.5763704/1‑94 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302540.3897970/1‑94 (MQ=60)
                                         CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      >  SRR4302540.5767037/1‑94 (MQ=60)
                                         AAAAGAAAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      <  SRR4302540.8601862/94‑1 (MQ=60)
                                          AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     >  SRR4302540.8718506/1‑94 (MQ=60)
                                          AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     >  SRR4302540.9332626/1‑94 (MQ=60)
                                           AAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGG                                                    <  SRR4302540.7969768/94‑1 (MQ=60)
                                             GATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  >  SRR4302540.8979669/1‑94 (MQ=60)
                                             AATAATAATTTTGGTGCGACCAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  <  SRR4302540.9895743/94‑1 (MQ=60)
                                              ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTT                                                 >  SRR4302540.551264/1‑94 (MQ=60)
                                              ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGCGTTACGACCAAAGGTGGTTT                                                 >  SRR4302540.551265/1‑94 (MQ=60)
                                               TAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTT                                                >  SRR4302540.9164506/1‑94 (MQ=60)
                                                AATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTA                                               >  SRR4302540.3032703/1‑94 (MQ=60)
                                                AATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTA                                               >  SRR4302540.1067983/1‑94 (MQ=60)
                                                 ATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAT                                              >  SRR4302540.3410508/1‑94 (MQ=60)
                                                 ATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAT                                              <  SRR4302540.9111012/94‑1 (MQ=60)
                                                   AATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTC                                            <  SRR4302540.9635462/94‑1 (MQ=60)
                                                   AATTTTGGTGCGAACAACCGATACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTC                                            <  SRR4302540.4597253/94‑1 (MQ=60)
                                                    ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302540.8064484/1‑94 (MQ=60)
                                                    ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           <  SRR4302540.1689451/94‑1 (MQ=60)
                                                    ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302540.5882002/1‑94 (MQ=60)
                                                    ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302540.5882003/1‑94 (MQ=60)
                                                    ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGAGGTTTTATTCA                                           >  SRR4302540.4167899/1‑94 (MQ=60)
                                                     TTTCGGTGCTAACAACCGGTCCTCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAG                                          <  SRR4302540.6474530/94‑1 (MQ=60)
                                                       TTGGTGCGAACAACGGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCA                                        <  SRR4302540.3397365/94‑1 (MQ=60)
                                                        TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       >  SRR4302540.2138672/1‑94 (MQ=60)
                                                        TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       <  SRR4302540.5507783/94‑1 (MQ=60)
                                                        TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       <  SRR4302540.7349367/94‑1 (MQ=60)
                                                        TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       <  SRR4302540.9696480/94‑1 (MQ=60)
                                                          GTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCttccaatctccgggtggtttcgacgaaggtgggttttagtagtgacga                                     >  SRR4302540.7650800/1‑46 (MQ=60)
                                                           TGCGAACACCCGCTACCCCCGCCAGACGCGCGG‑CTTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAA                                    <  SRR4302540.4587572/94‑1 (MQ=60)
                                                           TGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAA                                    <  SRR4302540.4587573/94‑1 (MQ=60)
                                                           TGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAA                                    <  SRR4302540.4587574/94‑1 (MQ=60)
                                                             CGAACAACCGGTACACCCGCCCGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCACAGGTGGTTTTATTCATCAGCAACC                                  >  SRR4302540.6136319/1‑94 (MQ=60)
                                                             CGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAC                                  <  SRR4302540.6637863/94‑1 (MQ=60)
                                                              GAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTTTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACC                                 <  SRR4302540.957714/94‑1 (MQ=60)
                                                              GAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACC                                 <  SRR4302540.7015538/94‑1 (MQ=60)
                                                              GAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACC                                 >  SRR4302540.3093037/1‑94 (MQ=60)
                                                               AACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                <  SRR4302540.921016/94‑1 (MQ=60)
                                                               AACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                >  SRR4302540.296517/1‑94 (MQ=60)
                                                               AACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                >  SRR4302540.7570034/1‑94 (MQ=60)
                                                                 CAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAA                              >  SRR4302540.8315062/1‑94 (MQ=60)
                                                                  AACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAG                             >  SRR4302540.7885144/1‑94 (MQ=60)
                                                                  AACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAG                             >  SRR4302540.9699858/1‑94 (MQ=60)
                                                                      GGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         <  SRR4302540.4811953/94‑1 (MQ=60)
                                                                      GGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         >  SRR4302540.8481648/1‑94 (MQ=60)
                                                                      GGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         <  SRR4302540.5062681/94‑1 (MQ=60)
                                                                       GTACACCCGCCAGACGCGCGGCC‑TGTTCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAAT                        <  SRR4302540.4357822/94‑1 (MQ=60)
                                                                       GTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAAT                        >  SRR4302540.8577927/1‑94 (MQ=60)
                                                                        TACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATC                       <  SRR4302540.5347948/94‑1 (MQ=60)
                                                                        TACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATC                       <  SRR4302540.5451176/94‑1 (MQ=60)
                                                                         ACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCA                      <  SRR4302540.9844068/94‑1 (MQ=60)
                                                                          CACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAG                     >  SRR4302540.1878427/1‑94 (MQ=60)
                                                                          CACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAG                     <  SRR4302540.6524795/94‑1 (MQ=60)
                                                                          CACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCACCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAG                     >  SRR4302540.9290174/1‑94 (MQ=60)
                                                                           CCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGA                    <  SRR4302540.4767538/94‑1 (MQ=60)
                                                                           ACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGA                    >  SRR4302540.2107320/1‑94 (MQ=60)
                                                                            CCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGAC                   <  SRR4302540.4260674/94‑1 (MQ=60)
                                                                             CCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  >  SRR4302540.1430510/1‑94 (MQ=60)
                                                                             CCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  <  SRR4302540.547516/94‑1 (MQ=60)
                                                                               cccacactggcgccC‑TCTTGGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGGGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACG                <  SRR4302540.3309122/80‑1 (MQ=60)
                                                                               GCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACG                <  SRR4302540.3309121/94‑1 (MQ=60)
                                                                                CCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGC               <  SRR4302540.2503127/94‑1 (MQ=60)
                                                                                  AGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             >  SRR4302540.4757908/1‑94 (MQ=60)
                                                                                  AGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             >  SRR4302540.599984/1‑94 (MQ=60)
                                                                                  AGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             <  SRR4302540.4553761/94‑1 (MQ=60)
                                                                                  AGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             >  SRR4302540.1332192/1‑94 (MQ=60)
                                                                                   GGCGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAC            <  SRR4302540.2926502/94‑1 (MQ=60)
                                                                                   GCCGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAC            <  SRR4302540.9101334/94‑1 (MQ=60)
                                                                                    ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           <  SRR4302540.5047589/94‑1 (MQ=60)
                                                                                    ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           <  SRR4302540.1450035/94‑1 (MQ=60)
                                                                                    ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           <  SRR4302540.9368101/94‑1 (MQ=60)
                                                                                    ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           <  SRR4302540.5047588/94‑1 (MQ=60)
                                                                                        ccggcC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGA       <  SRR4302540.8469566/89‑1 (MQ=60)
                                                                                          ggcC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGC     >  SRR4302540.797159/4‑94 (MQ=60)
                                                                                          ggcC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGC     >  SRR4302540.595582/4‑94 (MQ=60)
                                                                                           C‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCA   <  SRR4302540.9622316/94‑1 (MQ=60)
                                                                                             C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302540.2784068/1‑94 (MQ=60)
                                                                                             C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302540.5536732/1‑94 (MQ=60)
                                                                                             C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGCGAGCCAG  >  SRR4302540.5536731/1‑94 (MQ=60)
                                                                                             C‑TCTTCGTTCCCGCCAATCGCCAGGGCGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302540.3492874/1‑94 (MQ=60)
                                                                                             |                                                                                              
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980848‑1981033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑