Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,063,343 T→C A148A (GCA→GCG)  cobU ← cobinamide kinase/cobinamide phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,063,3430TC99.6% 886.4 / ‑5.0 277A148A (GCA→GCG) cobUcobinamide kinase/cobinamide phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (137/139);  total (137/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 289 total aligned reads displayed.

TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA  >  NC_000913/2063253‑2063432
                                                                                          |                                                                                         
tACTCCAATCCCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCgcgc                                                                                         <  1:7443185/93‑1 (MQ=255)
  cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                       <  2:2834781/93‑1 (MQ=255)
    ccAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                     <  1:9099234/93‑1 (MQ=255)
    ccAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                     <  1:1200744/93‑1 (MQ=255)
     cAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgac                                                                                    >  2:10043590/1‑93 (MQ=255)
      aaTCCCCGAAACCACCAGCCATACTTCATTTGCCGCATCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacg                                                                                   <  1:6719219/93‑1 (MQ=255)
       atccccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacga                                                                                  <  2:8097960/90‑1 (MQ=255)
        tccccGAACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 <  2:1958030/91‑1 (MQ=255)
        tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAgacgac                                                                                 <  1:9217884/93‑1 (MQ=255)
        tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 >  1:5949285/1‑93 (MQ=255)
         aCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAct                                                                                <  2:479710/93‑1 (MQ=255)
         aCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAct                                                                                <  1:3901695/93‑1 (MQ=255)
          cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               >  1:1691670/1‑93 (MQ=255)
          cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               >  1:2699054/1‑93 (MQ=255)
          cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGCCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               <  2:5625363/93‑1 (MQ=255)
           ccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActct                                                                              >  2:3892461/1‑93 (MQ=255)
            cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             <  1:7383572/93‑1 (MQ=255)
            cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             >  2:4710689/1‑93 (MQ=255)
              aacccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           <  1:8046768/90‑1 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           >  2:182190/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           >  2:6212200/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           >  1:7038192/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           <  2:4907710/93‑1 (MQ=255)
               aaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCgg                                                                          >  2:3735537/1‑93 (MQ=255)
                 ccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGca                                                                        <  1:2741010/93‑1 (MQ=255)
                 ccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGca                                                                        >  2:8826538/1‑93 (MQ=255)
                  caccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGCCTCTCCGGcac                                                                       >  1:3306821/1‑93 (MQ=255)
                  caccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcac                                                                       >  1:1016548/1‑93 (MQ=255)
                   accaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcaca                                                                      >  2:5229524/1‑93 (MQ=255)
                     caagcATACTTCATTTGCCGCAGCGGCCAACCTCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGGCTCTCCGGCACAAt                                                                    <  2:3059735/89‑1 (MQ=255)
                     caGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAAt                                                                    >  2:9523724/1‑93 (MQ=255)
                      aGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATc                                                                   <  2:5016981/93‑1 (MQ=255)
                       gCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcc                                                                  >  2:8445883/1‑93 (MQ=255)
                        ccATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATccc                                                                 <  1:7726418/93‑1 (MQ=255)
                         cATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcccc                                                                >  2:4078682/1‑93 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               >  1:4432618/1‑93 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               >  2:7332939/1‑93 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               >  1:2523681/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:2083027/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              <  2:7172225/93‑1 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:940750/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  2:4786533/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  2:4223820/1‑93 (MQ=255)
                            aCTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAtc                                                             >  1:3649357/1‑92 (MQ=255)
                               tCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTccc                                                          >  2:1302774/1‑93 (MQ=255)
                                cATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCa                                                         >  2:9740831/1‑93 (MQ=255)
                                  tttGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACt                                                       >  1:3268724/1‑93 (MQ=255)
                                   ttGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACtc                                                      >  1:5357185/1‑92 (MQ=255)
                                    tGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTc                                                     <  2:3622241/93‑1 (MQ=255)
                                    tGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTc                                                     <  1:2361919/93‑1 (MQ=255)
                                     gcggCAGCGGCCAACTGCTGATTTGCCCGCCCGGCCATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCg                                                    <  1:5155141/90‑1 (MQ=255)
                                     gCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCg                                                    >  1:4596030/1‑93 (MQ=255)
                                      ccGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGt                                                   <  2:8155902/93‑1 (MQ=255)
                                       cGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCGCAATCCCCATTCCCACTTCGtt                                                  <  2:7752885/93‑1 (MQ=255)
                                          aGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGt                                               >  2:5425948/1‑93 (MQ=255)
                                          aGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGt                                               <  2:1528934/93‑1 (MQ=255)
                                            cGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCa                                             >  1:6597663/1‑93 (MQ=255)
                                              gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           >  1:4247176/1‑93 (MQ=255)
                                              gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           >  1:4356091/1‑93 (MQ=255)
                                              gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           <  1:9929733/93‑1 (MQ=255)
                                                 aaCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAAt                                        >  2:1246472/1‑93 (MQ=255)
                                                  aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       >  2:5885841/1‑93 (MQ=255)
                                                  aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       >  2:3929619/1‑93 (MQ=255)
                                                     gctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACaa                                    <  1:8530272/93‑1 (MQ=255)
                                                     gctcATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACaa                                    <  1:888891/93‑1 (MQ=255)
                                                      ctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAc                                   <  2:1699613/93‑1 (MQ=255)
                                                       tgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                  >  2:6496993/1‑93 (MQ=255)
                                                       tgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                  >  1:3226780/1‑93 (MQ=255)
                                                       tgATGTACCCGCCCGGCAATATCACTAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                  <  1:9142124/93‑1 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                                >  2:9140160/1‑93 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             >  1:182014/1‑93 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             <  1:3575675/93‑1 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             <  2:3362524/93‑1 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             <  1:2006447/93‑1 (MQ=255)
                                                             aCCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCg                            <  2:7576519/93‑1 (MQ=255)
                                                              cccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCgg                           >  2:3230562/1‑93 (MQ=255)
                                                               ccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCACACTTCGTTAGTAACTAATACCACCTTTGCggg                          >  2:6118913/1‑93 (MQ=255)
                                                                 gcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGc                        <  2:6054760/93‑1 (MQ=255)
                                                                 gcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGc                        <  1:4833066/93‑1 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                       >  1:4532387/1‑93 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                       >  1:6380176/1‑93 (MQ=255)
                                                                   ccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCaa                      <  1:4156344/93‑1 (MQ=255)
                                                                       cAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGtt                  <  1:6529921/93‑1 (MQ=255)
                                                                         atatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTgg                <  1:9581948/93‑1 (MQ=255)
                                                                          tatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggc               >  2:8783489/1‑93 (MQ=255)
                                                                          tatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggc               >  2:2208483/1‑93 (MQ=255)
                                                                           atCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcg              >  2:8543052/1‑92 (MQ=255)
                                                                           atCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggca              >  2:2378534/1‑93 (MQ=255)
                                                                             cACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcagg            <  1:10004239/93‑1 (MQ=255)
                                                                               cGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAGTACAACCTTTGCGGGGCAACGTTggcaggca          <  2:6695716/93‑1 (MQ=255)
                                                                                gAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGgcaggcag         >  2:5680530/1‑93 (MQ=255)
                                                                                 aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        >  2:2595278/1‑93 (MQ=255)
                                                                                 aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        >  1:7145103/1‑93 (MQ=255)
                                                                                 aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTAGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgaagc        >  2:8509199/1‑93 (MQ=255)
                                                                                  aaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagca       >  1:5463803/1‑93 (MQ=255)
                                                                                  aaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagca       >  1:8675949/1‑93 (MQ=255)
                                                                                    aTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAAt     <  1:8157223/93‑1 (MQ=255)
                                                                                     tGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATc    <  1:5973524/93‑1 (MQ=255)
                                                                                      gTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCa   >  1:5803153/1‑93 (MQ=255)
                                                                                       tCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCaa  >  1:8271779/1‑93 (MQ=255)
                                                                                          |                                                                                         
TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA  >  NC_000913/2063253‑2063432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGAC  >  NC_000913/2063250‑2063436
                                                                                             |                                                                                             
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGC                                                                                               <  SRR4302540.1031748/94‑1 (MQ=60)
 TTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCG                                                                                              >  SRR4302540.521289/1‑94 (MQ=60)
  TTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGC                                                                                             >  SRR4302540.10033051/1‑94 (MQ=60)
  TTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGC                                                                                             <  SRR4302540.3555716/94‑1 (MQ=60)
  TTACCCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGACAATGTCGCGC                                                                                             <  SRR4302540.2855493/94‑1 (MQ=60)
   TACTCCAATCCCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCC                                                                                            <  SRR4302540.7526112/94‑1 (MQ=60)
   TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCC                                                                                            <  SRR4302540.7227621/94‑1 (MQ=60)
      TCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGA                                                                                         >  SRR4302540.8465885/1‑94 (MQ=60)
       CCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGAC                                                                                        <  SRR4302540.9208564/94‑1 (MQ=60)
       CCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGAC                                                                                        <  SRR4302540.9208563/94‑1 (MQ=60)
       CCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGAC                                                                                        <  SRR4302540.4969149/94‑1 (MQ=60)
       CCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGAC                                                                                        <  SRR4302540.1213093/94‑1 (MQ=60)
       CCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGAC                                                                                        <  SRR4302540.5330918/94‑1 (MQ=60)
        caatccccaaaccccCCAGCCATCCTCCATTGGCCGCACCGGCC‑ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGA                                                                                      <  SRR4302540.6666083/79‑1 (MQ=56)
        CAATNCCCGAAACCACCAGCCATNCTTCANTTGCCGCAGCGGCCANCTGCTGATTTANCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                       <  SRR4302540.4413414/94‑1 (MQ=60)
         AATCCCCGAAACCACCAGCCATACTTCATTTGCCGCATCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGA                                                                                      <  SRR4302540.6793370/94‑1 (MQ=60)
         AATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGA                                                                                      >  SRR4302540.9366475/1‑94 (MQ=60)
          ATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAC                                                                                     >  SRR4302540.6015073/1‑94 (MQ=60)
           TACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACT                                                                                    <  SRR4302540.9328454/94‑1 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   <  SRR4302540.7532297/94‑1 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   >  SRR4302540.2728185/1‑94 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   >  SRR4302540.6884262/1‑94 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   <  SRR4302540.3944021/94‑1 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   >  SRR4302540.1710015/1‑94 (MQ=60)
             CCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCT                                                                                  <  SRR4302540.9139158/94‑1 (MQ=60)
              CCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTC                                                                                 >  SRR4302540.3684727/1‑94 (MQ=60)
               CGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCC                                                                                <  SRR4302540.7465842/94‑1 (MQ=60)
               CGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCC                                                                                >  SRR4302540.7638970/1‑94 (MQ=60)
                GAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCG                                                                               >  SRR4302540.7116432/1‑94 (MQ=60)
                GAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCG                                                                               <  SRR4302540.4422513/94‑1 (MQ=60)
                 AACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGG                                                                              <  SRR4302540.8140246/94‑1 (MQ=60)
                 AAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGG                                                                              >  SRR4302540.3335996/1‑94 (MQ=60)
                 AAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGG                                                                              >  SRR4302540.8967032/1‑94 (MQ=60)
                    CCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                           <  SRR4302540.5909756/94‑1 (MQ=60)
                    CCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGCCTCTCCGGCAC                                                                           >  SRR4302540.3343040/1‑94 (MQ=60)
                    CCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                           >  SRR4302540.7365290/1‑94 (MQ=60)
                    CCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                           <  SRR4302540.2770606/94‑1 (MQ=60)
                    CCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                           >  SRR4302540.1026848/1‑94 (MQ=60)
                    CCACCAGCCATACTTCATTTGCAGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                           >  SRR4302540.3343044/1‑94 (MQ=60)
                     CCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACA                                                                          <  SRR4302540.1529323/94‑1 (MQ=60)
                       CCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAAT                                                                        <  SRR4302540.4620358/94‑1 (MQ=60)
                         AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                      <  SRR4302540.6433835/94‑1 (MQ=60)
                         AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                      <  SRR4302540.4507465/94‑1 (MQ=60)
                          gcccgactgcagttgccgcaggcgacgactgcggattcaccagccgggacATATCACGAAACTGTCGCGCCAGACGACTCTCCGGCACAATCCC                                                                     <  SRR4302540.4149788/44‑1 (MQ=60)
                          GCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCC                                                                     <  SRR4302540.4149789/94‑1 (MQ=60)
                          GCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCC                                                                     <  SRR4302540.4389430/94‑1 (MQ=60)
                           CCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCC                                                                    <  SRR4302540.7816607/94‑1 (MQ=60)
                            CATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCA                                                                   >  SRR4302540.3356997/1‑94 (MQ=60)
                            CATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCA                                                                   >  SRR4302540.4480640/1‑94 (MQ=60)
                            CATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCA                                                                   <  SRR4302540.2004307/94‑1 (MQ=60)
                            CATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCA                                                                   >  SRR4302540.2550927/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                  >  SRR4302540.3299651/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                  >  SRR4302540.950582/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                  >  SRR4302540.5492293/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                  >  SRR4302540.3791549/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                  >  SRR4302540.2105401/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGCCACAATCCCCAT                                                                  >  SRR4302540.3329363/1‑94 (MQ=60)
                              TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 >  SRR4302540.4254302/1‑94 (MQ=60)
                              TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 <  SRR4302540.3925410/94‑1 (MQ=60)
                              TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 <  SRR4302540.8168692/94‑1 (MQ=60)
                              TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 <  SRR4302540.2579452/94‑1 (MQ=60)
                              TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATC                                                                 >  SRR4302540.3689333/1‑94 (MQ=60)
                                CTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCC                                                               <  SRR4302540.9174472/94‑1 (MQ=60)
                                 TTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCC                                                              <  SRR4302540.9261221/94‑1 (MQ=60)
                                 CTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCC                                                              <  SRR4302540.6740347/94‑1 (MQ=60)
                                  TCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCA                                                             <  SRR4302540.708318/94‑1 (MQ=60)
                                  TCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCA                                                             >  SRR4302540.5503172/1‑94 (MQ=60)
                                    ATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACT                                                           >  SRR4302540.3304395/1‑94 (MQ=60)
                                    ATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACT                                                           >  SRR4302540.3304396/1‑94 (MQ=60)
                                     TTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTC                                                          >  SRR4302540.5416018/1‑94 (MQ=60)
                                       TGCCGCAGCGGCCACGTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                        <  SRR4302540.6713780/94‑1 (MQ=60)
                                       TGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                        >  SRR4302540.7219238/1‑94 (MQ=60)
                                       TGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                        <  SRR4302540.2387359/94‑1 (MQ=60)
                                       TGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                        <  SRR4302540.1094805/94‑1 (MQ=60)
                                       TGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                        >  SRR4302540.4645462/1‑94 (MQ=60)
                                        GCGGCAGCGGCCAACTGCTGATTTGCCCGCCCGGCCATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGT                                                       <  SRR4302540.5211099/94‑1 (MQ=60)
                                         CCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTT                                                      >  SRR4302540.2987252/1‑94 (MQ=60)
                                          CGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTA                                                     >  SRR4302540.6638933/1‑94 (MQ=60)
                                          CGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTA                                                     <  SRR4302540.3093978/94‑1 (MQ=60)
                                           GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                    >  SRR4302540.193424/1‑94 (MQ=60)
                                              GCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCA                                                 >  SRR4302540.6670721/1‑94 (MQ=60)
                                               CGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAC                                                <  SRR4302540.182901/94‑1 (MQ=60)
                                               CGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAC                                                <  SRR4302540.8866405/94‑1 (MQ=60)
                                                GGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACT                                               >  SRR4302540.4293384/1‑94 (MQ=60)
                                                GGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACT                                               >  SRR4302540.4403472/1‑94 (MQ=60)
                                                 GCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTA                                              <  SRR4302540.10045642/94‑1 (MQ=60)
                                                 GCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTA                                              >  SRR4302540.9673590/1‑94 (MQ=60)
                                                   CACCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAAT                                            <  SRR4302540.9257766/94‑1 (MQ=60)
                                                   CAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAAT                                            <  SRR4302540.7901492/94‑1 (MQ=60)
                                                    AACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATA                                           >  SRR4302540.5360492/1‑94 (MQ=60)
                                                    AACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATA                                           <  SRR4302540.10126431/94‑1 (MQ=60)
                                                     ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                          <  SRR4302540.8110588/94‑1 (MQ=60)
                                                       TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                        <  SRR4302540.4621582/94‑1 (MQ=60)
                                                        GCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAC                                       >  SRR4302540.8265776/1‑94 (MQ=60)
                                                        GCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAC                                       <  SRR4302540.8629385/94‑1 (MQ=60)
                                                        GCTCATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAC                                       <  SRR4302540.898335/94‑1 (MQ=60)
                                                         CTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACC                                      >  SRR4302540.3261861/1‑94 (MQ=60)
                                                         CTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACC                                      >  SRR4302540.8676942/1‑94 (MQ=60)
                                                         CTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACC                                      >  SRR4302540.10105204/1‑94 (MQ=60)
                                                          TGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCT                                     <  SRR4302540.2063770/94‑1 (MQ=60)
                                                          TGATGTACCCGCCCGGCAATATCACTAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCT                                     <  SRR4302540.9251951/94‑1 (MQ=60)
                                                            ATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTT                                   <  SRR4302540.3651248/94‑1 (MQ=60)
                                                              TTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGC                                 >  SRR4302540.183828/1‑94 (MQ=60)
                                                              TTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGC                                 <  SRR4302540.9409490/94‑1 (MQ=60)
                                                               TACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCG                                >  SRR4302540.6038638/1‑94 (MQ=60)
                                                               TACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCG                                <  SRR4302540.3614728/94‑1 (MQ=60)
                                                               TACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCG                                <  SRR4302540.2028159/94‑1 (MQ=60)
                                                               TACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGAG                                >  SRR4302540.6038637/1‑94 (MQ=60)
                                                                ACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAATCTTTGCGG                               >  SRR4302540.8148791/1‑94 (MQ=60)
                                                                ACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGG                               >  SRR4302540.9682242/1‑94 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                              <  SRR4302540.203849/94‑1 (MQ=60)
                                                                   CGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGC                            >  SRR4302540.2126614/1‑94 (MQ=60)
                                                                   CGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGC                            >  SRR4302540.572778/1‑94 (MQ=60)
                                                                    GCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCA                           <  SRR4302540.4884843/94‑1 (MQ=60)
                                                                    GCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCA                           >  SRR4302540.6451205/1‑94 (MQ=60)
                                                                    GCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCA                           <  SRR4302540.4884848/94‑1 (MQ=60)
                                                                    GCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCA                           >  SRR4302540.4581443/1‑94 (MQ=60)
                                                                     CCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAC                          >  SRR4302540.2460266/1‑94 (MQ=60)
                                                                     CCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAA                          >  SRR4302540.832629/1‑94 (MQ=60)
                                                                      CCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAC                         <  SRR4302540.10074436/94‑1 (MQ=60)
                                                                      CCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAC                         <  SRR4302540.4201373/94‑1 (MQ=60)
                                                                      CCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAC                         >  SRR4302540.8396543/1‑94 (MQ=60)
                                                                       CGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACG                        >  SRR4302540.1716339/1‑94 (MQ=60)
                                                                       CGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACG                        <  SRR4302540.4522156/94‑1 (MQ=60)
                                                                        GGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGT                       >  SRR4302540.1707431/1‑94 (MQ=60)
                                                                          CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                     <  SRR4302540.6602423/94‑1 (MQ=60)
                                                                          CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                     <  SRR4302540.5841879/94‑1 (MQ=60)
                                                                           AATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGG                    >  SRR4302540.2391857/1‑94 (MQ=60)
                                                                           AATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGG                    <  SRR4302540.8747300/94‑1 (MQ=60)
                                                                            ATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGC                   <  SRR4302540.9695092/94‑1 (MQ=60)
                                                                            ATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGC                   >  SRR4302540.4761524/1‑94 (MQ=60)
                                                                            ATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGC                   >  SRR4302540.652339/1‑94 (MQ=60)
                                                                             TATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCA                  <  SRR4302540.7489346/94‑1 (MQ=60)
                                                                              ATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAG                 >  SRR4302540.1115918/1‑94 (MQ=60)
                                                                              ATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAG                 <  SRR4302540.6546446/94‑1 (MQ=60)
                                                                                CACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGC               <  SRR4302540.10120765/94‑1 (MQ=60)
                                                                                 CCGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCA              <  SRR4302540.2127366/94‑1 (MQ=60)
                                                                                 ACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCA              <  SRR4302540.10021174/94‑1 (MQ=60)
                                                                                  CGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAG             <  SRR4302540.4222397/94‑1 (MQ=60)
                                                                                   GAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGC            <  SRR4302540.4777112/94‑1 (MQ=60)
                                                                                   GAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGC            >  SRR4302540.7224718/1‑94 (MQ=60)
                                                                                    AAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCA           >  SRR4302540.5523942/1‑94 (MQ=60)
                                                                                    AAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCA           >  SRR4302540.8776719/1‑94 (MQ=60)
                                                                                    AAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCA           <  SRR4302540.2601587/94‑1 (MQ=60)
                                                                                     AAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATCCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAA          >  SRR4302540.2753753/1‑94 (MQ=60)
                                                                                      AATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAAT         <  SRR4302540.7216950/94‑1 (MQ=60)
                                                                                      AATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAAT         <  SRR4302540.4164897/94‑1 (MQ=60)
                                                                                       ATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATC        <  SRR4302540.8251929/94‑1 (MQ=60)
                                                                                        TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       <  SRR4302540.6039644/94‑1 (MQ=60)
                                                                                        TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       >  SRR4302540.5867272/1‑94 (MQ=60)
                                                                                         GTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA      >  SRR4302540.8367881/1‑94 (MQ=60)
                                                                                          TCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAAC     <  SRR4302540.6061129/94‑1 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG    <  SRR4302540.3219663/94‑1 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG    <  SRR4302540.3969776/94‑1 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG    >  SRR4302540.1271898/1‑94 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTGCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG    <  SRR4302540.4479529/94‑1 (MQ=60)
                                                                                            GCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGA   >  SRR4302540.8891562/1‑94 (MQ=60)
                                                                                            GCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGA   >  SRR4302540.8891561/1‑94 (MQ=60)
                                                                                            GCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGA   <  SRR4302540.1761763/94‑1 (MQ=60)
                                                                                             CGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGAC  <  SRR4302540.8862684/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGAC  >  NC_000913/2063250‑2063436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑