Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,924,563	T→C	N78N (AAT→AAC)	ygdH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,924,563	0	T	C	92.3%	1086.9 / 58.3	409	N78N (AAT→AAC)	ygdH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/13); new base C (164/208); total (186/223)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-02

Only 100 of 452 total aligned reads displayed.

ACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTA  >  NC_000913/2924471‑2924652

 ccGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATc                                                                                          >  2:2034640/1‑93 (MQ=255)

 ccGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACGGATTAATc                                                                                          >  2:10001187/1‑93 (MQ=255)

  cGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAccc                                                                                         >  2:7016039/1‑93 (MQ=255)

   gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccc                                                                                        >  2:3273690/1‑93 (MQ=255)

   gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccc                                                                                        <  1:8390493/93‑1 (MQ=255)

   gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTCAACGCGGCGTAAAGCTGGAACTGATTAAcccc                                                                                        >  1:5414280/1‑93 (MQ=255)

    aTAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACcccc                                                                                       >  2:8482051/1‑93 (MQ=255)

     tAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccccc                                                                                      <  1:2997838/93‑1 (MQ=255)

     tAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccccc                                                                                      >  2:8055879/1‑93 (MQ=255)

      aaCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCg                                                                                     <  2:9086529/93‑1 (MQ=255)

      aaCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAACCCCCg                                                                                     >  2:3864054/1‑93 (MQ=255)

       aCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCgg                                                                                    >  1:3268474/1‑93 (MQ=255)

       aCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCgg                                                                                    >  1:10043578/1‑93 (MQ=255)

            aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAagag                                                                               >  1:2308961/1‑93 (MQ=255)

            aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAagag                                                                               <  2:12962/93‑1 (MQ=255)

            aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAagag                                                                               <  2:3654884/93‑1 (MQ=255)

             aaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAgg                                                                              >  1:3920245/1‑93 (MQ=255)

               gAATTGCTGTCTCGTTTTGCAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTACCCCCCCGGAAGAGGCt                                                                            <  2:8959463/93‑1 (MQ=255)

               gAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCt                                                                            >  1:9201143/1‑93 (MQ=255)

                aaTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCtt                                                                           >  1:8664801/1‑93 (MQ=255)

                 aTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCttt                                                                          <  1:4245191/93‑1 (MQ=255)

                 aTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCttt                                                                          >  1:1642692/1‑93 (MQ=255)

                 aTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCttt                                                                          <  2:6403500/93‑1 (MQ=255)

                  ttGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCtttt                                                                         >  1:4516750/1‑93 (MQ=255)

                    gCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGt                                                                       <  1:7652229/93‑1 (MQ=255)

                    gCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGt                                                                       <  2:8211744/93‑1 (MQ=255)

                      tGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCg                                                                     >  1:5568839/1‑93 (MQ=255)

                      tGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCg                                                                     >  2:5463313/1‑93 (MQ=255)

                       gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAGCTGATTAACCCCCCGGAAGAGGCTTTTGTCGa                                                                    >  2:3530100/1‑93 (MQ=255)

                       gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGa                                                                    <  1:5202667/93‑1 (MQ=255)

                       gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGa                                                                    <  1:1758437/93‑1 (MQ=255)

                        tctcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAt                                                                   >  1:8271237/1‑93 (MQ=255)

                         ctcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATg                                                                  <  2:5926627/93‑1 (MQ=255)

                         ctcGTTTTGAAAATTTCGAGATTAACGTCTTGCGACGTGAACGCGGCGTAAAGCTGGAACTGAGTAACCCCCCGGAAGAGGCTTTTGTCGATg                                                                  <  1:8195123/93‑1 (MQ=255)

                          tcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATgg                                                                 <  1:760021/93‑1 (MQ=255)

                          tcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATgg                                                                 >  2:4702806/1‑93 (MQ=255)

                           cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATggg                                                                >  2:3979533/1‑93 (MQ=255)

                            gTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGc                                                               >  2:7648714/1‑93 (MQ=255)

                            gTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGc                                                               <  2:9847480/93‑1 (MQ=255)

                             ttttGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCg                                                              >  2:1163116/1‑93 (MQ=255)

                                 gAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAatt                                                          <  2:7525059/93‑1 (MQ=255)

                                  aaaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAatta                                                         <  2:9797656/93‑1 (MQ=255)

                                  aaaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAatta                                                         <  1:956072/93‑1 (MQ=255)

                                   aaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAattat                                                        >  2:2667447/1‑93 (MQ=255)

                                   aaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAattat                                                        >  2:2111743/1‑93 (MQ=255)

                                    aaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAattatt                                                       >  2:2817953/1‑93 (MQ=255)

                                     aTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTc                                                      <  2:1726952/93‑1 (MQ=255)

                                      tttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                     <  1:7962482/93‑1 (MQ=255)

                                      tttCGATATTAACGTCGTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                     <  1:7164957/93‑1 (MQ=255)

                                       ttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcgc                                                    <  1:7795352/93‑1 (MQ=255)

                                           atatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCtt                                                >  1:6144630/1‑93 (MQ=255)

                                           atatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCtt                                                <  1:6828771/93‑1 (MQ=255)

                                           atatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCtt                                                <  1:9819776/93‑1 (MQ=255)

                                            tatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCTGAAGAGGCTTTTGTCGATGGGCGAATTATTGGCGGttt                                               >  2:2473273/1‑93 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             >  2:1600677/1‑93 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             <  2:927046/93‑1 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             >  2:5771454/1‑93 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             <  1:4091076/93‑1 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGCAACTGATTAGCCCCCCGGAAGAGTCTTTTGTCGATGGGCGAATTATTCGCCCTTTGc                                             <  2:6474693/93‑1 (MQ=255)

                                              tcaaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             <  2:9593721/91‑1 (MQ=255)

                                               tAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCa                                            <  1:3728431/93‑1 (MQ=255)

                                               tAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCa                                            <  2:88543/93‑1 (MQ=255)

                                                aaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAg                                           >  2:7201622/1‑93 (MQ=255)

                                                aaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAg                                           >  1:1884887/1‑93 (MQ=255)

                                                     cTTGCGCCGGGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCaa                                      <  1:8599963/93‑1 (MQ=255)

                                                      ttGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAc                                     <  1:2750325/93‑1 (MQ=255)

                                                      ttGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAc                                     >  1:3456970/1‑93 (MQ=255)

                                                           ccGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGtt                                <  1:1958466/93‑1 (MQ=255)

                                                           ccGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGtt                                >  2:563282/1‑93 (MQ=255)

                                                            cGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTc                               >  1:1190859/1‑93 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGTAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              <  2:417228/93‑1 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              >  2:6642541/1‑93 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              >  2:1936585/1‑93 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              <  1:9011742/93‑1 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              <  1:6580626/93‑1 (MQ=255)

                                                              tGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgc                             <  1:1392636/93‑1 (MQ=255)

                                                              tGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgc                             >  1:9385160/1‑93 (MQ=255)

                                                                aaCGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCgg                           >  2:7739101/1‑93 (MQ=255)

                                                                aaCGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCgg                           <  2:6831313/93‑1 (MQ=255)

                                                                   gcggcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTcc                        <  1:6051903/93‑1 (MQ=255)

                                                                   gcggcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTcc                        <  2:3417031/93‑1 (MQ=255)

                                                                     ggcgTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTg                      <  2:9396626/93‑1 (MQ=255)

                                                                     ggcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTg                      >  2:8739025/1‑93 (MQ=255)

                                                                      gcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGc                     <  2:8656202/93‑1 (MQ=255)

                                                                      gcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGc                     >  2:4954264/1‑93 (MQ=255)

                                                                            aGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACa               >  1:3560501/1‑93 (MQ=255)

                                                                             gCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAt              >  2:4802514/1‑93 (MQ=255)

                                                                              cTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAtt             >  1:5869323/1‑93 (MQ=255)

                                                                              cTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAtt             <  1:4310529/93‑1 (MQ=255)

                                                                               tGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtc            >  1:7437672/1‑93 (MQ=255)

                                                                                ggAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtct           >  1:2627055/1‑93 (MQ=255)

                                                                                 gAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtctc          <  1:4874977/93‑1 (MQ=255)

                                                                                  aaCTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTctct         <  1:2159263/93‑1 (MQ=255)

                                                                                    cTGATTACCCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  2:4430021/93‑1 (MQ=255)

                                                                                    cTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  2:7827685/93‑1 (MQ=255)

                                                                                    cTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  2:5164894/93‑1 (MQ=255)

                                                                                    atGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  1:1440820/92‑1 (MQ=255)

                                                                                     tGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTct      >  1:6350786/1‑92 (MQ=255)

                                                                                      gATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGt     >  2:2357697/1‑93 (MQ=255)

                                                                                        ttAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGttt   <  2:614464/93‑1 (MQ=255)

ACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTA  >  NC_000913/2924471‑2924652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 229 total aligned reads displayed.

GACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGG  >  NC_000913/2924470‑2924655

GACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAC                                                                                              >  SRR4302540.1338141/1‑94 (MQ=60)

 ACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACC                                                                                             <  SRR4302540.3667004/94‑1 (MQ=60)

 ACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACC                                                                                             <  SRR4302540.9344009/94‑1 (MQ=60)

  CCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCC                                                                                            <  SRR4302540.6757920/94‑1 (MQ=60)

   CGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCC                                                                                           <  SRR4302540.8376371/94‑1 (MQ=60)

   CGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCC                                                                                           >  SRR4302540.8305146/1‑94 (MQ=60)

   CGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCC                                                                                           >  SRR4302540.8305145/1‑94 (MQ=60)

   CGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCC                                                                                           <  SRR4302540.7046097/94‑1 (MQ=60)

   CGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCC                                                                                           <  SRR4302540.7019649/94‑1 (MQ=60)

    GATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTTATTAACCCCC                                                                                          >  SRR4302540.2509383/1‑94 (MQ=60)

    GATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCC                                                                                          <  SRR4302540.4305809/94‑1 (MQ=60)

    GATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCC                                                                                          >  SRR4302540.8019116/1‑94 (MQ=60)

    GATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCC                                                                                          <  SRR4302540.8487855/94‑1 (MQ=60)

    GATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCC                                                                                          >  SRR4302540.7807010/1‑94 (MQ=60)

    GATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCC                                                                                          <  SRR4302540.8487856/94‑1 (MQ=60)

     ATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCC                                                                                         <  SRR4302540.9443210/94‑1 (MQ=60)

     ATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCC                                                                                         <  SRR4302540.431774/94‑1 (MQ=60)

     ATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCC                                                                                         <  SRR4302540.306341/94‑1 (MQ=60)

      TAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCG                                                                                        <  SRR4302540.4332972/94‑1 (MQ=60)

      TAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCG                                                                                        <  SRR4302540.3428827/94‑1 (MQ=60)

      TAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCG                                                                                        <  SRR4302540.3030868/94‑1 (MQ=60)

      TAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAA‑CCCCCGG                                                                                       <  SRR4302540.2079252/94‑1 (MQ=60)

       AACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGG                                                                                       <  SRR4302540.7402385/94‑1 (MQ=60)

       AACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGG                                                                                       >  SRR4302540.3304145/1‑94 (MQ=60)

       AACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGG                                                                                       >  SRR4302540.10160344/1‑94 (MQ=60)

         CAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAA                                                                                     <  SRR4302540.3437568/94‑1 (MQ=60)

          AGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAG                                                                                    >  SRR4302540.172251/1‑94 (MQ=60)

          AGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGACG                                                                                    >  SRR4302540.4051701/1‑94 (MQ=60)

          AGCAAAGAATTGCTGTCTCGATTTGAAAATTTTGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAAATGATTAACCCCCCGGAAG                                                                                    >  SRR4302540.3358725/1‑94 (MQ=60)

            CAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAG                                                                                  >  SRR4302540.2333817/1‑94 (MQ=60)

            CAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAG                                                                                  >  SRR4302540.8569242/1‑94 (MQ=60)

             AAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGG                                                                                 >  SRR4302540.3962676/1‑94 (MQ=60)

             AAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGG                                                                                 <  SRR4302540.8277897/94‑1 (MQ=60)

              AAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGC                                                                                >  SRR4302540.9803367/1‑94 (MQ=60)

              AAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGC                                                                                >  SRR4302540.2764572/1‑94 (MQ=60)

              AAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGC                                                                                >  SRR4302540.2764571/1‑94 (MQ=60)

              AAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGC                                                                                <  SRR4302540.9173010/94‑1 (MQ=60)

               AGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTATCCCCCCGGAAGAGGCT                                                                               <  SRR4302540.527387/94‑1 (MQ=60)

               AGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCT                                                                               >  SRR4302540.9311534/1‑94 (MQ=60)

                GAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTT                                                                              <  SRR4302540.5461935/94‑1 (MQ=60)

                GAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTT                                                                              >  SRR4302540.8765443/1‑94 (MQ=60)

                GAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTT                                                                              >  SRR4302540.1500347/1‑94 (MQ=60)

                GAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTT                                                                              >  SRR4302540.8765442/1‑94 (MQ=60)

                 AATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTT                                                                             <  SRR4302540.86964/94‑1 (MQ=60)

                 AATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTT                                                                             >  SRR4302540.1660642/1‑94 (MQ=60)

                 AATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTT                                                                             >  SRR4302540.6107737/1‑94 (MQ=60)

                  ATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTT                                                                            >  SRR4302540.4565677/1‑94 (MQ=60)

                   TTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTG                                                                           <  SRR4302540.3347151/94‑1 (MQ=60)

                    TGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGT                                                                          <  SRR4302540.1965562/94‑1 (MQ=60)

                     GCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTC                                                                         <  SRR4302540.5133436/94‑1 (MQ=60)

                     GCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTC                                                                         <  SRR4302540.7738372/94‑1 (MQ=60)

                      CTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCG                                                                        >  SRR4302540.3889165/1‑94 (MQ=60)

                      CTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCG                                                                        >  SRR4302540.5630161/1‑94 (MQ=60)

                       TGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGA                                                                       >  SRR4302540.6648377/1‑94 (MQ=60)

                        TTCTCGTTTTGAAAAGTTGGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTTGACCTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.3661540/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.1051162/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.2049137/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.1051163/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.4439483/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.8178854/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      >  SRR4302540.4182189/1‑94 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      >  SRR4302540.5249855/1‑94 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.5259328/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.3661539/94‑1 (MQ=60)

                        GTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAT                                                                      <  SRR4302540.1777497/94‑1 (MQ=60)

                         TCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATG                                                                     <  SRR4302540.7887154/94‑1 (MQ=60)

                         TCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATG                                                                     >  SRR4302540.211847/1‑94 (MQ=60)

                          CTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGG                                                                    >  SRR4302540.9675370/1‑94 (MQ=60)

                          CTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGG                                                                    <  SRR4302540.5518091/94‑1 (MQ=60)

                          CTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGG                                                                    >  SRR4302540.6537337/1‑94 (MQ=60)

                          CTCGTTTTGAAAATTTCGAGATTAACGTCTTGCGACGTGAACGCGGCGTAAAGCTGGAACTGAGTAACCCCCCGGAAGAGGCTTTTGTCGATGG                                                                    <  SRR4302540.8290357/94‑1 (MQ=60)

                           TCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGG                                                                   <  SRR4302540.768109/94‑1 (MQ=60)

                            CGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGC                                                                  <  SRR4302540.5596885/94‑1 (MQ=60)

                            CGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGG                                                                  >  SRR4302540.9536652/1‑94 (MQ=60)

                            CGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGC                                                                  >  SRR4302540.8032217/1‑94 (MQ=60)

                             GTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTACCCCCCCGGAAGAGGCTTTTGTCGATGGGCG                                                                 <  SRR4302540.422025/94‑1 (MQ=60)

                             GTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGGG                                                                 >  SRR4302540.5613327/1‑94 (MQ=60)

                             GTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCG                                                                 >  SRR4302540.6160192/1‑94 (MQ=60)

                             GTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCG                                                                 >  SRR4302540.357591/1‑94 (MQ=60)

                              TTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGA                                                                >  SRR4302540.9167125/1‑94 (MQ=60)

                               TTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAA                                                               >  SRR4302540.1902805/1‑94 (MQ=60)

                                  GAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTA                                                            <  SRR4302540.604246/94‑1 (MQ=60)

                                   AAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAGCTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTAT                                                           <  SRR4302540.1861501/94‑1 (MQ=60)

                                   AAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGTAAGAGGCTTTTGTCGATGGGCGAATTAT                                                           >  SRR4302540.3510976/1‑94 (MQ=60)

                                   AAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTAT                                                           >  SRR4302540.3743066/1‑94 (MQ=60)

                                   AAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTAT                                                           <  SRR4302540.965982/94‑1 (MQ=60)

                                    AAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATT                                                          <  SRR4302540.7963041/94‑1 (MQ=60)

                                    AAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATT                                                          >  SRR4302540.4768346/1‑94 (MQ=60)

                                     AATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTACCCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTC                                                         <  SRR4302540.7122709/94‑1 (MQ=60)

                                     AATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTC                                                         <  SRR4302540.2322000/94‑1 (MQ=60)

                                      ATTTCGATATTAACGTCGTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCG                                                        <  SRR4302540.8324630/94‑1 (MQ=60)

                                       attccATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGC                                                       <  SRR4302540.7244835/89‑1 (MQ=60)

                                       TTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGC                                                       <  SRR4302540.9890314/94‑1 (MQ=60)

                                       TTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGGTGGGCGAATTATTCGC                                                       >  SRR4302540.6243872/1‑94 (MQ=60)

                                       TTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGC                                                       <  SRR4302540.8055287/94‑1 (MQ=60)

                                        TTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCG                                                      <  SRR4302540.7886467/94‑1 (MQ=60)

                                        TTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCG                                                      >  SRR4302540.8377424/1‑94 (MQ=60)

                                        TTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCG                                                      >  SRR4302540.3830381/1‑94 (MQ=60)

                                        TTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCG                                                      <  SRR4302540.7886466/94‑1 (MQ=60)

                                          CGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCT                                                    >  SRR4302540.1934613/1‑94 (MQ=60)

                                           GATATTAACGTCTTGCGNNGTGAACGCGGCGTAAAGCTGNAANTGATTNACCCNCCGGAAGAGGCTTTNGTNNATGNGCGAATTNTNNGNGNTN                                                   >  SRR4302540.6906395/1‑94 (MQ=60)

                                           GATATTAACGTCTTGCGCCGTGAACGGGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTACTCGCGCTT                                                   >  SRR4302540.7717599/1‑94 (MQ=60)

                                           GATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTT                                                   >  SRR4302540.6213068/1‑94 (MQ=60)

                                           GATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTT                                                   >  SRR4302540.6213069/1‑94 (MQ=60)

                                           GATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTT                                                   >  SRR4302540.7717598/1‑94 (MQ=60)

                                           GATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAA‑CCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTT                                                  <  SRR4302540.5287868/94‑1 (MQ=60)

                                            ATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTT                                                  <  SRR4302540.6666064/94‑1 (MQ=60)

                                            ATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTT                                                  <  SRR4302540.6904381/94‑1 (MQ=60)

                                            ATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTT                                                  <  SRR4302540.9934672/94‑1 (MQ=60)

                                            ATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTT                                                  >  SRR4302540.1021766/1‑94 (MQ=60)

                                            ATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTT                                                  >  SRR4302540.5573802/1‑94 (MQ=60)

                                             TATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTG                                                 <  SRR4302540.5978787/94‑1 (MQ=60)

                                             TATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTG                                                 >  SRR4302540.1445127/1‑94 (MQ=60)

                                               TTAACGTCTTGCGCCGTGCACGAGGAGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGGGAATTATTCGCGCTTT‑CAG                                              >  SRR4302540.2861904/1‑94 (MQ=60)

                                               TTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCA                                               <  SRR4302540.4135210/94‑1 (MQ=60)

                                               TTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCA                                               >  SRR4302540.4720837/1‑94 (MQ=60)

                                                TAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAG                                              >  SRR4302540.1905529/1‑94 (MQ=60)

                                                TAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAG                                              <  SRR4302540.3769105/94‑1 (MQ=60)

                                                TAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAG                                              >  SRR4302540.2252586/1‑94 (MQ=60)

                                                 AACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGGAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGG                                             >  SRR4302540.9123010/1‑94 (MQ=60)

                                                 AACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGG                                             >  SRR4302540.4012102/1‑94 (MQ=60)

                                                 AACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGG                                             <  SRR4302540.4263720/94‑1 (MQ=60)

                                                   CGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCCGGCC                                           >  SRR4302540.8984145/1‑94 (MQ=60)

                                                      CTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAC                                        >  SRR4302540.3099085/1‑94 (MQ=60)

                                                      CTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAC                                        <  SRR4302540.14534/94‑1 (MQ=60)

                                                      CTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAC                                        >  SRR4302540.3494599/1‑94 (MQ=60)

                                                      CTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAC                                        <  SRR4302540.10094195/94‑1 (MQ=60)

                                                      CTTGCGCCGGGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAC                                        <  SRR4302540.8699921/94‑1 (MQ=60)

                                                       TTGCGGCGGGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACA                                       <  SRR4302540.2780043/94‑1 (MQ=60)

                                                       TTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACT                                       <  SRR4302540.2959080/94‑1 (MQ=60)

                                                       TTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACA                                       <  SRR4302540.2780044/94‑1 (MQ=60)

                                                        TGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTT                                      <  SRR4302540.9766088/94‑1 (MQ=60)

                                                         GCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTG                                     <  SRR4302540.2916477/94‑1 (MQ=60)

                                                         GCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTG                                     <  SRR4302540.1393244/94‑1 (MQ=60)

                                                         GCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTG                                     <  SRR4302540.7453165/94‑1 (MQ=60)

                                                            CCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTC                                  >  SRR4302540.5732529/1‑94 (MQ=60)

                                                            CCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTC                                  >  SRR4302540.1203133/1‑94 (MQ=60)

                                                            CCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTC                                  >  SRR4302540.6772231/1‑94 (MQ=60)

                                                            CCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTC                                  <  SRR4302540.9477602/94‑1 (MQ=60)

                                                            CCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTC                                  <  SRR4302540.1979755/94‑1 (MQ=60)

                                                            CCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTC                                  <  SRR4302540.4395410/94‑1 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                >  SRR4302540.6345237/1‑94 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                >  SRR4302540.9496868/1‑94 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                <  SRR4302540.9119849/94‑1 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                >  SRR4302540.9109376/1‑94 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                <  SRR4302540.2536393/94‑1 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                >  SRR4302540.5711129/1‑94 (MQ=60)

                                                              GTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGC                                <  SRR4302540.6653520/94‑1 (MQ=60)

                                                               TGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCG                               <  SRR4302540.6807194/94‑1 (MQ=60)

                                                               TGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCG                               <  SRR4302540.1407553/94‑1 (MQ=60)

                                                                GAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGG                              >  SRR4302540.1678317/1‑94 (MQ=60)

                                                                GAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTGTGTAGGCCAACTTGTTCGCCG                              >  SRR4302540.9402613/1‑94 (MQ=60)

                                                                 AACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGT                             <  SRR4302540.5281983/94‑1 (MQ=60)

                                                                  ACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTC                            >  SRR4302540.840463/1‑94 (MQ=60)

                                                                  ACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCAAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTC                            >  SRR4302540.4015530/1‑94 (MQ=60)

                                                                   CGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCC                           <  SRR4302540.3716403/94‑1 (MQ=60)

                                                                   CGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCC                           <  SRR4302540.4840879/94‑1 (MQ=60)

                                                                    GCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAGTTATTAGCGCTTTGCAGGCCAACTTGGTCGCGGaacg                          >  SRR4302540.5240411/1‑90 (MQ=60)

                                                                    GCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCT                          <  SRR4302540.6119160/94‑1 (MQ=60)

                                                                    GCGGCGGAAAGCTGGAACTCATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCT                          <  SRR4302540.3771153/94‑1 (MQ=60)

                                                                     CGGCGTAAAGCTGGAACTGATTAACCCCCCGTAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTG                         <  SRR4302540.10113746/94‑1 (MQ=60)

                                                                      GGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGC                        >  SRR4302540.6303515/1‑94 (MQ=60)

                                                                      GGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGC                        >  SRR4302540.4591344/1‑94 (MQ=60)

                                                                       GCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCG                       >  SRR4302540.1873713/1‑94 (MQ=60)

                                                                         GTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTG                     >  SRR4302540.3590062/1‑94 (MQ=60)

                                                                         GTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCATG                     <  SRR4302540.1614524/94‑1 (MQ=60)

                                                                          TAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGA                    >  SRR4302540.4335554/1‑94 (MQ=60)

                                                                           AAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGAC                   >  SRR4302540.8702312/1‑94 (MQ=60)

                                                                            AAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACA                  <  SRR4302540.8311392/94‑1 (MQ=60)

                                                                            AAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACA                  <  SRR4302540.1835266/94‑1 (MQ=60)

                                                                            AAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACA                  >  SRR4302540.3599396/1‑94 (MQ=60)

                                                                             AGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAT                 >  SRR4302540.2521173/1‑94 (MQ=60)

                                                                             AGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAT                 <  SRR4302540.6571560/94‑1 (MQ=60)

                                                                             AGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAT                 <  SRR4302540.7708366/94‑1 (MQ=60)

                                                                              GCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATT                >  SRR4302540.5934203/1‑94 (MQ=60)

                                                                               CTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTC               >  SRR4302540.8595301/1‑94 (MQ=60)

                                                                               CTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTC               >  SRR4302540.7520546/1‑94 (MQ=60)

                                                                               CTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTC               <  SRR4302540.4357451/94‑1 (MQ=60)

                                                                               CTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTC               <  SRR4302540.950294/94‑1 (MQ=60)

                                                                                TGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCT              >  SRR4302540.1622961/1‑94 (MQ=60)

                                                                                TGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCT              >  SRR4302540.2655470/1‑94 (MQ=60)

                                                                                TGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCT              >  SRR4302540.2078813/1‑94 (MQ=60)

                                                                                TGGAACTGATTAA‑CCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTC             >  SRR4302540.5984061/1‑94 (MQ=60)

                                                                                 GGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTC             <  SRR4302540.7513095/94‑1 (MQ=60)

                                                                                  GAACTGTTTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCT            <  SRR4302540.2892703/94‑1 (MQ=60)

                                                                                  GAACTGATTACCCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCT            <  SRR4302540.2226749/94‑1 (MQ=60)

                                                                                  GAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCT            <  SRR4302540.4927294/94‑1 (MQ=60)

                                                                                   AACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGCCCTGCGTGACATTCTCTT           >  SRR4302540.8842433/1‑94 (MQ=60)

                                                                                    ACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTC          <  SRR4302540.1300875/94‑1 (MQ=60)

                                                                                    ACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTC          <  SRR4302540.3064078/94‑1 (MQ=60)

                                                                                    ACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTC          <  SRR4302540.7562306/94‑1 (MQ=60)

                                                                                    ACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTC          <  SRR4302540.7562305/94‑1 (MQ=60)

                                                                                     CTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCG         <  SRR4302540.76807/94‑1 (MQ=60)

                                                                                     ATGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCG         <  SRR4302540.1456461/94‑1 (MQ=60)

                                                                                      TGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGT        >  SRR4302540.6392286/1‑94 (MQ=60)

                                                                                      TGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGT        >  SRR4302540.1272178/1‑94 (MQ=60)

                                                                                         TTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTA     <  SRR4302540.1472347/94‑1 (MQ=60)

                                                                                           AACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCATGCGTGACATTCTCTTCGTTTACG   <  SRR4302540.9321169/94‑1 (MQ=60)

                                                                                            ACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGG  <  SRR4302540.6392641/94‑1 (MQ=60)

                                                                                            ACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGG  >  SRR4302540.3006129/1‑94 (MQ=60)

GACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGG  >  NC_000913/2924470‑2924655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`