Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,979,546 Δ1 bp coding (1047/1386 nt) yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,979,5450G.99.8% 2080.6 / ‑3.9 459coding (1046/1386 nt)yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (254/203);  total (255/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 487 total aligned reads displayed.

TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TT‑G‑CTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633
                                                                                              |                                                                                        
ttACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCTg                                                                                          >  2:8674883/1‑93 (MQ=255)
ttACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCTg                                                                                          >  2:165575/1‑93 (MQ=255)
ttACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCTg                                                                                          >  1:2217465/1‑93 (MQ=255)
ttACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TT‑GTCTCATGCCTg                                                                                          >  1:3072933/1‑93 (MQ=255)
 tACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCTga                                                                                         <  2:9329041/93‑2 (MQ=255)
   ccGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCTgaac                                                                                       <  1:4113390/93‑4 (MQ=255)
      gcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTAc                                                                                   >  1:4815310/1‑93 (MQ=255)
       cggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTAca                                                                                  <  1:6080303/93‑1 (MQ=255)
        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCGT‑GAACTAcat                                                                                 >  2:8246924/1‑93 (MQ=255)
        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTAcat                                                                                 >  1:8383792/1‑93 (MQ=255)
            aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCAtt                                                                             >  2:4438277/1‑93 (MQ=255)
            aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCAtt                                                                             <  1:3029056/93‑1 (MQ=255)
             tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTc                                                                            >  2:208824/1‑93 (MQ=255)
             tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTc                                                                            <  2:6492712/93‑1 (MQ=255)
                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCa                                                                         <  2:3072126/93‑1 (MQ=255)
                    aGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATcc                                                                     <  1:8116974/93‑1 (MQ=255)
                     gTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTT‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCg                                                                    <  2:8422105/93‑1 (MQ=255)
                       ttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  <  2:1330622/93‑1 (MQ=255)
                          ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCg                                                               >  2:4688009/1‑93 (MQ=255)
                           cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              <  1:6987904/93‑1 (MQ=255)
                             tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgt                                                            >  1:1650235/1‑93 (MQ=255)
                             tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgg                                                            >  2:2436787/1‑92 (MQ=255)
                               aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                                          >  2:315454/1‑93 (MQ=255)
                               aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                                          <  2:9799475/93‑1 (MQ=255)
                                cGGCGTCCCGCTTGCGGGTGGGGCAGTACCAATTGCTATCCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  2:416023/93‑1 (MQ=255)
                                cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  1:5710886/93‑1 (MQ=255)
                                cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  1:3634938/93‑1 (MQ=255)
                                 ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        >  1:9955192/1‑93 (MQ=255)
                                 ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        >  1:2862304/1‑93 (MQ=255)
                                 ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        <  1:7212869/93‑1 (MQ=255)
                                  gCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                                       >  1:7586550/1‑93 (MQ=255)
                                   cGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgt                                                      <  2:7056473/93‑1 (MQ=255)
                                   cGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgt                                                      <  2:85058/93‑1 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  2:3762646/93‑1 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:1049052/93‑1 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:8801672/93‑1 (MQ=255)
                                      aCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtcta                                                   <  1:2738970/93‑1 (MQ=255)
                                       ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                                  <  1:6708848/93‑1 (MQ=255)
                                       ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                                  >  1:1860192/1‑93 (MQ=255)
                                        cGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacg                                                 <  2:4664871/93‑1 (MQ=255)
                                        cGGTCGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacg                                                 <  1:4221094/93‑1 (MQ=255)
                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  2:2601101/1‑93 (MQ=255)
                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  1:9685640/1‑93 (MQ=255)
                                           ttGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtct                                              <  1:136186/93‑1 (MQ=255)
                                             gCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtctac                                            >  1:5833940/1‑93 (MQ=255)
                                              cGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                           <  1:7941577/93‑1 (MQ=255)
                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          <  1:3898496/93‑1 (MQ=255)
                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  2:7857550/1‑93 (MQ=255)
                                                ggTGTGGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGt                                         >  2:9155017/1‑93 (MQ=255)
                                                    tgGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     >  1:1188308/1‑93 (MQ=255)
                                                    tgGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     <  1:2477108/93‑1 (MQ=255)
                                                    tgGCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     <  2:1889585/93‑1 (MQ=255)
                                                     ggCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                                    >  2:515680/1‑93 (MQ=255)
                                                      gCAGTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGc                                   >  2:1033283/1‑93 (MQ=255)
                                                      gCAGGATCTATTGCTATTCTGCTAA‑TTGG‑CTCCTGCCT‑GAACTACCTCGTTCCCCATCCGCAGCGTGTGTTTGTCTACGGCTACAGGGcacag                                   >  2:5047434/1‑89 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:10009527/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  2:7590185/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:8462640/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  2:6362431/1‑93 (MQ=255)
                                                           aTCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              >  2:390601/1‑93 (MQ=255)
                                                           aTCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              >  1:7299545/1‑93 (MQ=255)
                                                           aTCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              >  2:5633265/1‑93 (MQ=255)
                                                           aTCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACGACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              <  2:1129435/93‑1 (MQ=255)
                                                            tCTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCtt                             >  2:7952651/1‑93 (MQ=255)
                                                             cTATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            <  2:5729796/93‑1 (MQ=255)
                                                              tATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                           >  2:2997869/1‑93 (MQ=255)
                                                              tATTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                           >  2:3571715/1‑93 (MQ=255)
                                                               aTTGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          <  1:196583/93‑1 (MQ=255)
                                                                ttGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         >  2:7196282/1‑93 (MQ=255)
                                                                ttGCTATTCTGCTAA‑TTGG‑CTCAGGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         <  2:5506378/93‑1 (MQ=255)
                                                                 tGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTTCATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        >  1:3719070/1‑93 (MQ=255)
                                                                 tGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        <  2:6086815/93‑1 (MQ=255)
                                                                 tGCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        >  2:5400595/1‑93 (MQ=255)
                                                                  gCTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgggg                       >  1:6318349/1‑93 (MQ=255)
                                                                   cTATTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      >  1:4504682/1‑93 (MQ=255)
                                                                     aTTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg                    <  1:9353908/93‑1 (MQ=255)
                                                                     aTTCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg                    >  2:6576045/1‑93 (MQ=255)
                                                                      ttCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   <  1:8932578/93‑1 (MQ=255)
                                                                      ttCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   <  2:675258/93‑1 (MQ=255)
                                                                       tCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  >  2:4292870/1‑93 (MQ=255)
                                                                       tCTGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  >  2:6988467/1‑93 (MQ=255)
                                                                         tGCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                >  2:9596022/1‑93 (MQ=255)
                                                                          gCTAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGcc               >  2:8942434/1‑93 (MQ=255)
                                                                            tAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCag             >  1:3263380/1‑92 (MQ=255)
                                                                            tAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             <  1:9101408/93‑1 (MQ=255)
                                                                            tAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             <  1:5037133/93‑1 (MQ=255)
                                                                            tAA‑TTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             <  1:6234238/93‑1 (MQ=255)
                                                                                ttgg‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCTGGGATGGTGCCATGGt          >  2:8149047/4‑93 (MQ=255)
                                                                                ttgg‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  1:5131970/4‑93 (MQ=255)
                                                                                ttgg‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          <  1:5393039/90‑1 (MQ=255)
                                                                                 tgg‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGtt         <  1:4557417/91‑1 (MQ=255)
                                                                                  gg‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        <  2:5895665/92‑1 (MQ=255)
                                                                                  gg‑CTCATGCCT‑GAACTACATCATTCCCAATCAGCAGCGTGTGTTTTTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        >  2:1550682/2‑93 (MQ=255)
                                                                                      tCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtgtg     <  1:4422028/93‑1 (MQ=255)
                                                                                      tCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtgtg     <  2:1754400/93‑1 (MQ=255)
                                                                                        aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  2:4785191/1‑93 (MQ=255)
                                                                                        aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  1:6516035/1‑93 (MQ=255)
                                                                                        aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  1:5285299/1‑93 (MQ=255)
                                                                                         tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTCata  >  2:2657578/1‑93 (MQ=255)
                                                                                         tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGCGCTTCGCGCGTTGGTGCCATGGTTTGTGagt  >  1:7744553/1‑91 (MQ=255)
                                                                                              |                                                                                        
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAA‑TT‑G‑CTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 246 total aligned reads displayed.
TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979455‑3979639
                                                                                            |                                                                                             
TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTGAA                                                                                              <  SRR4302540.5194326/94‑1 (MQ=60)
  CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           <  SRR4302540.2896930/94‑1 (MQ=60)
  CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           <  SRR4302540.4157801/94‑1 (MQ=60)
  CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           <  SRR4302540.9009488/94‑1 (MQ=60)
  CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           <  SRR4302540.5247737/94‑1 (MQ=60)
  CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGGACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           >  SRR4302540.9584626/1‑94 (MQ=60)
   CGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTA                                                                                          >  SRR4302540.3595921/1‑94 (MQ=60)
    GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTTTTCTAATTGGCTCATGCCT‑GAACTAC                                                                                         >  SRR4302540.4190824/1‑94 (MQ=60)
    GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAC                                                                                         >  SRR4302540.4866850/1‑94 (MQ=60)
    GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAC                                                                                         <  SRR4302540.9241106/94‑1 (MQ=60)
     GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACA                                                                                        <  SRR4302540.6391308/94‑1 (MQ=60)
      CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       >  SRR4302540.8481063/1‑94 (MQ=60)
      CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       <  SRR4302540.3052431/94‑1 (MQ=60)
      CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       <  SRR4302540.5769598/94‑1 (MQ=60)
      CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       <  SRR4302540.6147924/94‑1 (MQ=60)
      CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       <  SRR4302540.7808358/94‑1 (MQ=60)
       GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTANTTGGCTCATGCCT‑GAACTACATC                                                                                      >  SRR4302540.4577281/1‑94 (MQ=60)
       GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      >  SRR4302540.6271929/1‑94 (MQ=60)
       GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      <  SRR4302540.1800120/94‑1 (MQ=60)
        GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                                     <  SRR4302540.4784888/94‑1 (MQ=60)
           ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCCTTC                                                                                  >  SRR4302540.4274858/1‑94 (MQ=60)
           ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                  <  SRR4302540.3062326/94‑1 (MQ=60)
           ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                  <  SRR4302540.8530465/94‑1 (MQ=60)
           ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                  <  SRR4302540.3680182/94‑1 (MQ=60)
             GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCC                                                                                <  SRR4302540.5870268/94‑1 (MQ=60)
             GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCC                                                                                <  SRR4302540.727350/94‑1 (MQ=60)
              GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                               >  SRR4302540.8523943/1‑94 (MQ=60)
               CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                              >  SRR4302540.6114441/1‑94 (MQ=60)
                GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCGCAAT                                                                             >  SRR4302540.8456800/1‑94 (MQ=60)
                GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302540.4128550/1‑94 (MQ=60)
                GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302540.241193/94‑1 (MQ=60)
                 AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATC                                                                            <  SRR4302540.1297326/94‑1 (MQ=60)
                  AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           >  SRR4302540.7057396/1‑94 (MQ=60)
                   AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          <  SRR4302540.8211170/94‑1 (MQ=60)
                   AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          <  SRR4302540.4971945/94‑1 (MQ=60)
                   AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          <  SRR4302540.769030/94‑1 (MQ=60)
                        CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     <  SRR4302540.10109650/94‑1 (MQ=60)
                        CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     <  SRR4302540.3768541/94‑1 (MQ=60)
                        CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     <  SRR4302540.7085082/94‑1 (MQ=60)
                          CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302540.7065483/94‑1 (MQ=60)
                          CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302540.2906195/94‑1 (MQ=60)
                           GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  >  SRR4302540.1668233/1‑94 (MQ=60)
                              ACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTT                                                               <  SRR4302540.9914770/94‑1 (MQ=60)
                              ACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTT                                                               >  SRR4302540.5620690/1‑94 (MQ=60)
                              ACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTT                                                               >  SRR4302540.2034836/1‑94 (MQ=60)
                               CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GCACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302540.2893337/1‑94 (MQ=60)
                               CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302540.2893338/1‑94 (MQ=60)
                               CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302540.10071392/1‑94 (MQ=60)
                               CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302540.83832/94‑1 (MQ=60)
                               CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302540.5773754/94‑1 (MQ=60)
                               CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302540.3674727/94‑1 (MQ=60)
                                GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             <  SRR4302540.7293276/94‑1 (MQ=60)
                                GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             <  SRR4302540.7293277/94‑1 (MQ=60)
                                GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             >  SRR4302540.8205006/1‑94 (MQ=60)
                                GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             <  SRR4302540.3255532/94‑1 (MQ=60)
                                GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             >  SRR4302540.7671467/1‑94 (MQ=60)
                                 GCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGT                                                            <  SRR4302540.3865964/94‑1 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCCATCCGCAGCGTGGGTTTGTCT                                                          >  SRR4302540.8723987/1‑94 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302540.4572333/1‑94 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302540.7381952/94‑1 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302540.1059720/94‑1 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302540.1060013/94‑1 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302540.539892/1‑94 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302540.8903427/94‑1 (MQ=60)
                                   GTACCGGTTGCGGGTGTGGCAGCATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302540.6344246/1‑94 (MQ=60)
                                    TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         >  SRR4302540.5102274/1‑94 (MQ=60)
                                     TCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        <  SRR4302540.1715007/94‑1 (MQ=60)
                                     TCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        <  SRR4302540.7299303/94‑1 (MQ=60)
                                     ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        <  SRR4302540.2768536/94‑1 (MQ=60)
                                     ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        >  SRR4302540.669124/1‑94 (MQ=60)
                                     ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        >  SRR4302540.1880593/1‑94 (MQ=60)
                                     ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        <  SRR4302540.5745089/94‑1 (MQ=60)
                                      CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       <  SRR4302540.6782884/94‑1 (MQ=60)
                                      CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       <  SRR4302540.4561945/94‑1 (MQ=60)
                                      CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       >  SRR4302540.3099997/1‑94 (MQ=60)
                                      CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       >  SRR4302540.7356743/1‑94 (MQ=60)
                                      CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACA                                                       <  SRR4302540.1163836/94‑1 (MQ=60)
                                       CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                      >  SRR4302540.1948435/1‑94 (MQ=60)
                                       CGGTCGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                      <  SRR4302540.4267048/94‑1 (MQ=60)
                                        GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302540.9799584/1‑94 (MQ=60)
                                          TTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTA                                                   <  SRR4302540.137615/94‑1 (MQ=60)
                                           TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302540.3478695/94‑1 (MQ=60)
                                           TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302540.3999860/94‑1 (MQ=60)
                                           TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  >  SRR4302540.3997339/1‑94 (MQ=60)
                                           TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  >  SRR4302540.5898449/1‑94 (MQ=60)
                                             CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                <  SRR4302540.8034126/94‑1 (MQ=60)
                                             CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                <  SRR4302540.1061318/94‑1 (MQ=60)
                                             CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGATCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                <  SRR4302540.4228714/94‑1 (MQ=60)
                                              GGGTGTGGCAGTATCTATTTCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302540.7899946/94‑1 (MQ=60)
                                              GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302540.7396792/94‑1 (MQ=60)
                                              GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302540.3940802/94‑1 (MQ=60)
                                              GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302540.5291815/94‑1 (MQ=60)
                                               GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302540.3715440/1‑94 (MQ=60)
                                                GTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGC                                             >  SRR4302540.9441260/1‑94 (MQ=60)
                                                 TGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACCTCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCC                                            <  SRR4302540.6307295/94‑1 (MQ=60)
                                                 TGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCC                                            <  SRR4302540.4404804/94‑1 (MQ=60)
                                                  GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACTTCTCCCGTGCCA                                           >  SRR4302540.1350358/1‑94 (MQ=60)
                                                  GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           <  SRR4302540.6299759/94‑1 (MQ=60)
                                                  GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           >  SRR4302540.1200559/1‑94 (MQ=60)
                                                  GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           >  SRR4302540.6877199/1‑94 (MQ=60)
                                                  GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           <  SRR4302540.3439032/94‑1 (MQ=60)
                                                   TGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                          <  SRR4302540.2521469/94‑1 (MQ=60)
                                                   TGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                          <  SRR4302540.2503779/94‑1 (MQ=60)
                                                    GGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGC                                         <  SRR4302540.390376/94‑1 (MQ=60)
                                                    GGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGC                                         <  SRR4302540.913225/94‑1 (MQ=60)
                                                    GGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATCCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGC                                         <  SRR4302540.3728488/94‑1 (MQ=60)
                                                     GCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                                        >  SRR4302540.5260659/1‑94 (MQ=60)
                                                     GCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                                        <  SRR4302540.4479410/94‑1 (MQ=60)
                                                      CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       <  SRR4302540.9175733/94‑1 (MQ=60)
                                                      CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       <  SRR4302540.9863634/94‑1 (MQ=60)
                                                      CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302540.2205861/1‑94 (MQ=60)
                                                      CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       <  SRR4302540.5406533/94‑1 (MQ=60)
                                                      CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302540.10131578/1‑94 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302540.4897373/1‑94 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302540.10126081/1‑94 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302540.8560903/1‑94 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      <  SRR4302540.6856616/94‑1 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      <  SRR4302540.5170667/94‑1 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302540.7550858/1‑94 (MQ=60)
                                                       AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302540.6495933/1‑94 (MQ=60)
                                                        GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302540.8413526/1‑94 (MQ=60)
                                                        GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302540.1497124/1‑94 (MQ=60)
                                                        GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302540.8943272/1‑94 (MQ=60)
                                                         TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302540.4253754/1‑94 (MQ=60)
                                                         TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302540.7381023/1‑94 (MQ=60)
                                                         TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302540.2729615/1‑94 (MQ=60)
                                                          ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   >  SRR4302540.728569/1‑94 (MQ=60)
                                                          ATCTATTGCTATTCTGCTAATTGGCTCATGACT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   <  SRR4302540.2823180/94‑1 (MQ=60)
                                                           TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                                  >  SRR4302540.3130130/1‑94 (MQ=60)
                                                            CTATTTCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302540.3195668/94‑1 (MQ=60)
                                                            CTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGCCTACAGTGCCAGCGTGCTTCC                                 >  SRR4302540.3752579/1‑94 (MQ=60)
                                                             TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                <  SRR4302540.8127883/94‑1 (MQ=60)
                                                             TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302540.1048262/1‑94 (MQ=60)
                                                              ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302540.5631591/1‑94 (MQ=60)
                                                              ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302540.2682373/1‑94 (MQ=60)
                                                              ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               <  SRR4302540.198531/94‑1 (MQ=60)
                                                               TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              <  SRR4302540.3168588/94‑1 (MQ=60)
                                                                TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             >  SRR4302540.6388706/1‑94 (MQ=60)
                                                                TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             >  SRR4302540.8629401/1‑94 (MQ=60)
                                                                TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             >  SRR4302540.9679463/1‑94 (MQ=60)
                                                                TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             <  SRR4302540.8165956/94‑1 (MQ=60)
                                                                 GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302540.4553510/1‑94 (MQ=60)
                                                                  CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302540.7821003/94‑1 (MQ=60)
                                                                  CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302540.8884579/1‑94 (MQ=60)
                                                                  CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302540.848371/94‑1 (MQ=60)
                                                                  CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302540.6166681/1‑94 (MQ=60)
                                                                  CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302540.9051145/94‑1 (MQ=60)
                                                                  CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302540.584102/94‑1 (MQ=60)
                                                                   TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302540.8942196/1‑94 (MQ=60)
                                                                    ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302540.4354620/1‑94 (MQ=60)
                                                                    ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         <  SRR4302540.9465316/94‑1 (MQ=60)
                                                                    ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302540.1580124/1‑94 (MQ=60)
                                                                    ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302540.5416619/1‑94 (MQ=60)
                                                                     TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        >  SRR4302540.7747250/1‑94 (MQ=60)
                                                                     TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302540.9036914/94‑1 (MQ=60)
                                                                     TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302540.6059323/94‑1 (MQ=60)
                                                                     TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        >  SRR4302540.6389296/1‑94 (MQ=60)
                                                                      TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       >  SRR4302540.1211726/1‑94 (MQ=60)
                                                                      TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       <  SRR4302540.587430/94‑1 (MQ=60)
                                                                      TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       <  SRR4302540.2347350/94‑1 (MQ=60)
                                                                        TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     >  SRR4302540.1913903/1‑94 (MQ=60)
                                                                        TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     <  SRR4302540.9754407/94‑1 (MQ=60)
                                                                        TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGGGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGCTGGTGGC                     >  SRR4302540.3100371/1‑94 (MQ=60)
                                                                          CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302540.6765739/1‑94 (MQ=60)
                                                                           TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  <  SRR4302540.6303575/94‑1 (MQ=60)
                                                                           TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  <  SRR4302540.4531527/94‑1 (MQ=60)
                                                                           TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  <  SRR4302540.5091623/94‑1 (MQ=60)
                                                                           TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  >  SRR4302540.172398/1‑94 (MQ=60)
                                                                           TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTATGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  >  SRR4302540.9027189/1‑94 (MQ=60)
                                                                            AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACATTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 >  SRR4302540.7843775/1‑94 (MQ=60)
                                                                            AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 >  SRR4302540.3064140/1‑94 (MQ=60)
                                                                            AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGGGCCATGG                 >  SRR4302540.7901152/1‑94 (MQ=60)
                                                                              ttgGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTT               <  SRR4302540.5452338/91‑1 (MQ=60)
                                                                              TG‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              <  SRR4302540.6112517/94‑1 (MQ=60)
                                                                              TG‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              <  SRR4302540.5105451/94‑1 (MQ=60)
                                                                              TG‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              <  SRR4302540.4606681/94‑1 (MQ=60)
                                                                               G‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             >  SRR4302540.4537270/1‑94 (MQ=60)
                                                                               G‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             <  SRR4302540.2826659/94‑1 (MQ=60)
                                                                                 GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGT            <  SRR4302540.1376958/94‑1 (MQ=60)
                                                                                  CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302540.10099756/1‑94 (MQ=60)
                                                                                  CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302540.9884835/1‑94 (MQ=60)
                                                                                  CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGCCTACAGTGCCAGCGTGATTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302540.2605951/1‑94 (MQ=60)
                                                                                   TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          >  SRR4302540.198884/1‑94 (MQ=60)
                                                                                   TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          <  SRR4302540.4469942/94‑1 (MQ=60)
                                                                                   TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          >  SRR4302540.1781696/1‑94 (MQ=60)
                                                                                    CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTGCGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         >  SRR4302540.6588425/1‑94 (MQ=60)
                                                                                    CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         >  SRR4302540.6588424/1‑94 (MQ=60)
                                                                                    CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         <  SRR4302540.1977585/94‑1 (MQ=60)
                                                                                    CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         >  SRR4302540.5343051/1‑94 (MQ=60)
                                                                                     ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        <  SRR4302540.9288590/94‑1 (MQ=60)
                                                                                      TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT       >  SRR4302540.2837696/1‑94 (MQ=60)
                                                                                      TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT       >  SRR4302540.9367155/1‑94 (MQ=60)
                                                                                      TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT       <  SRR4302540.4721728/94‑1 (MQ=60)
                                                                                       GCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATT      >  SRR4302540.3380877/1‑94 (MQ=60)
                                                                                          ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATTGTGCCATGGTTTGTGATATTGA    >  SRR4302540.1170702/3‑94 (MQ=60)
                                                                                          ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    >  SRR4302540.9196227/3‑94 (MQ=60)
                                                                                          ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    <  SRR4302540.2364029/92‑1 (MQ=60)
                                                                                          ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    >  SRR4302540.9185783/3‑94 (MQ=60)
                                                                                           TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   >  SRR4302540.9258752/1‑94 (MQ=60)
                                                                                            GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  <  SRR4302540.4607837/94‑1 (MQ=60)
                                                                                            |                                                                                             
TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979455‑3979639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑