Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,171,379 C→T A240V (GCC→GTC)  birA → biotin‑[acetyl‑CoA carboxylase] holoenzyme synthetase and biotin operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,171,3790CT95.5% 1598.6 / 25.3 495A240V (GCC→GTC) birAbiotin‑[acetyl‑CoA carboxylase] holoenzyme synthetase and biotin operon repressor
Reads supporting (aligned to +/- strand):  ref base C (15/7);  new base T (275/197);  total (290/205)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 522 total aligned reads displayed.

CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAAT  >  NC_000913/4171289‑4171470
                                                                                          |                                                                                           
cAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCa                                                                                           <  1:1274141/93‑1 (MQ=255)
cAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCa                                                                                           <  2:2675554/93‑1 (MQ=255)
cAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCa                                                                                           <  1:3718563/93‑1 (MQ=255)
 aaTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAt                                                                                          <  1:1705577/93‑1 (MQ=255)
 aaTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAt                                                                                          >  1:2177972/1‑93 (MQ=255)
  aTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATg                                                                                         >  2:5300035/1‑93 (MQ=255)
  aTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATAAATCTCGAGCGTAATACGTTGGCGGTCATg                                                                                         >  1:3189288/1‑93 (MQ=255)
   tGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGc                                                                                        >  1:7083455/1‑93 (MQ=255)
   tGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGc                                                                                        <  1:4577171/93‑1 (MQ=255)
   tGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGc                                                                                        >  1:1108904/1‑93 (MQ=255)
       ccGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAAt                                                                                    <  1:4245391/93‑1 (MQ=255)
        cGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATa                                                                                   <  1:3360770/93‑1 (MQ=255)
         gtgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATAc                                                                                  <  2:1469227/93‑1 (MQ=255)
         gtgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATAc                                                                                  >  1:918634/1‑93 (MQ=255)
          tgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGGCATGCTAATACg                                                                                 >  2:6307692/1‑93 (MQ=255)
            ttGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTg                                                                               >  1:6362098/1‑93 (MQ=255)
              gAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGaa                                                                             >  1:5032560/1‑93 (MQ=255)
               aaGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAAt                                                                            >  2:5673805/1‑93 (MQ=255)
               aaGAGAGTGTCGTTAATCAGGGGGGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAAt                                                                            >  2:10045354/1‑93 (MQ=255)
                   gagTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACg                                                                        >  2:5470092/1‑93 (MQ=255)
                   gagTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACg                                                                        <  1:4733382/93‑1 (MQ=255)
                   gagTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACg                                                                        <  1:4790006/93‑1 (MQ=255)
                     gtgtCGTTAATCAGGGGTGGATCACGCTGCAGGAGGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATTCTAATACGTGAATTACGtg                                                                      <  1:196085/93‑1 (MQ=255)
                     gtgtCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtg                                                                      <  2:3725604/93‑1 (MQ=255)
                       gtCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgct                                                                    >  1:6679825/1‑93 (MQ=255)
                        tCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgctg                                                                   <  1:9752758/93‑1 (MQ=255)
                           ttAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGt                                                                <  2:1210564/93‑1 (MQ=255)
                           ttAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTAAATTACGTGCTGCGt                                                                <  2:5065386/93‑1 (MQ=255)
                            tAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGtt                                                               >  2:957080/1‑93 (MQ=255)
                              aTCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTgg                                                             <  2:2469658/93‑1 (MQ=255)
                               tCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCTTGCTAATACGTGAATTAAGTGCTGCGGTGGa                                                            >  1:7421717/1‑93 (MQ=255)
                               tCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGa                                                            >  2:4243663/1‑93 (MQ=255)
                               tCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGa                                                            >  2:7252430/1‑93 (MQ=255)
                               tCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGa                                                            >  1:7160169/1‑93 (MQ=255)
                               tCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACCTGAATTACGTGCTGCGTTGGa                                                            >  1:8360121/1‑93 (MQ=255)
                                  ggggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAct                                                         >  1:1578272/1‑93 (MQ=255)
                                  ggggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAct                                                         >  1:2582652/1‑93 (MQ=255)
                                  ggggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAct                                                         >  1:7861656/1‑93 (MQ=255)
                                   gggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAActc                                                        <  1:4104756/93‑1 (MQ=255)
                                    ggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAActct                                                       >  2:9367845/1‑93 (MQ=255)
                                    ggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAActct                                                       >  1:9371004/1‑93 (MQ=255)
                                      tGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTc                                                     <  1:8629637/93‑1 (MQ=255)
                                         aTCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGaa                                                  >  1:7288274/1‑93 (MQ=255)
                                          tCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAAc                                                 >  1:3520752/1‑93 (MQ=255)
                                           cACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACa                                                <  1:7745756/93‑1 (MQ=255)
                                           cACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACa                                                <  1:5404663/93‑1 (MQ=255)
                                            aCGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACaa                                               >  2:8371935/1‑93 (MQ=255)
                                              gCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACaaga                                             <  2:2920099/93‑1 (MQ=255)
                                              gCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACaaga                                             <  2:8148414/93‑1 (MQ=255)
                                                tGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACaagaag                                           <  1:4377518/93‑1 (MQ=255)
                                                 gCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGCACTCTTCGAACAAGAAgg                                          >  1:5644972/1‑93 (MQ=255)
                                                 gCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAgg                                          >  1:2361779/1‑93 (MQ=255)
                                                 gCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAgg                                          >  1:5761569/1‑93 (MQ=255)
                                                   aGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAt                                        <  2:3416722/93‑1 (MQ=255)
                                                    ggAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGCAGGAtt                                       >  2:9746209/1‑93 (MQ=255)
                                                    ggAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAtt                                       <  2:6773979/93‑1 (MQ=255)
                                                    ggAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAtt                                       >  2:4024526/1‑93 (MQ=255)
                                                    ggAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAtt                                       >  2:5939136/1‑93 (MQ=255)
                                                     gAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTg                                      >  2:1666313/1‑93 (MQ=255)
                                                     gAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTg                                      >  2:4755774/1‑93 (MQ=255)
                                                      aaGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTgg                                     <  1:6491775/93‑1 (MQ=255)
                                                       aGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTTGAACTCTTCGAACAAGAAGGATTgtc                                    >  2:9018942/1‑91 (MQ=255)
                                                        gCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTGGAACAAGAAGGATTGGCa                                   <  2:5728075/93‑1 (MQ=255)
                                                        gCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCa                                   >  2:86386/1‑93 (MQ=255)
                                                        gCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCCTCGAACCAGAAGGAttatca                                   >  1:6208111/1‑89 (MQ=255)
                                                           gggATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCt                                <  2:2291594/93‑1 (MQ=255)
                                                           gggATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCt                                >  2:2861707/1‑93 (MQ=255)
                                                           gggATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCt                                <  2:4330283/93‑1 (MQ=255)
                                                             gATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTa                              <  1:5955439/93‑1 (MQ=255)
                                                             gATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTa                              >  1:8178615/1‑93 (MQ=255)
                                                              aTCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAt                             >  2:641126/1‑93 (MQ=255)
                                                              aTCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAt                             >  2:6888177/1‑93 (MQ=255)
                                                              aTCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAt                             >  1:8031678/1‑93 (MQ=255)
                                                              aTCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAt                             >  2:4627681/1‑93 (MQ=255)
                                                                cAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACCAGAAGGATTGGCACCTTATCt                           >  1:6008281/1‑93 (MQ=255)
                                                                cAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCt                           <  2:6125216/93‑1 (MQ=255)
                                                                cAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCt                           >  1:3136046/1‑93 (MQ=255)
                                                                 aaTCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGACACCTTATCTg                          >  2:3183678/1‑93 (MQ=255)
                                                                  aTCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGt                         <  2:7528693/93‑1 (MQ=255)
                                                                   tctcGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTc                        >  1:9521254/1‑93 (MQ=255)
                                                                   tctcGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTc                        <  2:384337/93‑1 (MQ=255)
                                                                   tctcGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTc                        >  1:2851959/1‑93 (MQ=255)
                                                                   tctcGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTc                        >  2:4973912/1‑93 (MQ=255)
                                                                      cGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTcgcg                     <  2:3773003/93‑1 (MQ=255)
                                                                      cGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACATTATCTGTcgcg                     >  2:7600201/1‑93 (MQ=255)
                                                                       gATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCgcgc                    >  2:8736842/1‑93 (MQ=255)
                                                                       gATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCgcgc                    >  2:2027074/1‑93 (MQ=255)
                                                                         tCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTg                  >  1:6783987/1‑93 (MQ=255)
                                                                         tCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGATCTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTg                  <  1:693421/93‑1 (MQ=255)
                                                                          cGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTgg                 >  1:5141113/1‑93 (MQ=255)
                                                                           gTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTggg                >  2:3018039/1‑93 (MQ=255)
                                                                              aTACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaa             >  1:8899864/1‑93 (MQ=255)
                                                                              aTACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaa             >  2:9236581/1‑93 (MQ=255)
                                                                               tACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaaa            <  1:8953650/93‑1 (MQ=255)
                                                                               tACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaaa            >  2:702669/1‑93 (MQ=255)
                                                                                 cGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCCCCTTATCTGTCGCGCTGGGAAAAGc          <  1:2079797/93‑1 (MQ=255)
                                                                                    tGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTgg       <  2:1302655/93‑1 (MQ=255)
                                                                                     ggcggtCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGa      >  1:6995270/1‑93 (MQ=255)
                                                                                      gcggtCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGAt     >  2:7360321/1‑93 (MQ=255)
                                                                                       cggtCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATa    >  2:4419395/1‑93 (MQ=255)
                                                                                          |                                                                                           
CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAAT  >  NC_000913/4171289‑4171470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 275 total aligned reads displayed.
GGCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTT  >  NC_000913/4171287‑4171472
                                                                                            |                                                                                              
GGCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTC                                                                                               <  SRR4302540.8231684/94‑1 (MQ=60)
 GCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCA                                                                                              >  SRR4302540.6406435/1‑94 (MQ=60)
  CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAT                                                                                             <  SRR4302540.9625797/94‑1 (MQ=60)
  CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAT                                                                                             <  SRR4302540.3759137/94‑1 (MQ=60)
  CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAT                                                                                             <  SRR4302540.1287271/94‑1 (MQ=60)
  CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAT                                                                                             >  SRR4302540.2237344/1‑94 (MQ=60)
   AATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATG                                                                                            >  SRR4302540.4211891/1‑94 (MQ=60)
   AATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATG                                                                                            >  SRR4302540.6155562/1‑94 (MQ=60)
   AATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATG                                                                                            >  SRR4302540.71111/1‑94 (MQ=60)
   AATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATG                                                                                            <  SRR4302540.1724016/94‑1 (MQ=60)
   AATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATAAATCTCGAGCGTAATACGTTGGCGGTCATG                                                                                            >  SRR4302540.3223931/1‑94 (MQ=60)
    ATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGC                                                                                           >  SRR4302540.1120366/1‑94 (MQ=60)
    ATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGC                                                                                           >  SRR4302540.3340439/1‑94 (MQ=60)
    ATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGC                                                                                           >  SRR4302540.5783137/1‑94 (MQ=60)
    ATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGC                                                                                           >  SRR4302540.7554489/1‑94 (MQ=60)
    ATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGC                                                                                           >  SRR4302540.1465112/1‑94 (MQ=60)
     TGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCT                                                                                          <  SRR4302540.4626538/94‑1 (MQ=60)
     TGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCT                                                                                          <  SRR4302540.2030765/94‑1 (MQ=60)
       CGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAA                                                                                        >  SRR4302540.2982411/1‑94 (MQ=60)
         CCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATA                                                                                      <  SRR4302540.4291578/94‑1 (MQ=60)
         CCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATA                                                                                      <  SRR4302540.7161860/94‑1 (MQ=60)
         CCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATA                                                                                      >  SRR4302540.6346802/1‑94 (MQ=60)
          CGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATAC                                                                                     >  SRR4302540.4647388/1‑94 (MQ=60)
          CGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATAC                                                                                     >  SRR4302540.3551914/1‑94 (MQ=60)
          CGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATAC                                                                                     <  SRR4302540.3397563/94‑1 (MQ=60)
           GTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACG                                                                                    >  SRR4302540.1961016/1‑94 (MQ=60)
           GTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACG                                                                                    <  SRR4302540.7379765/94‑1 (MQ=60)
           GTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACG                                                                                    >  SRR4302540.593693/1‑94 (MQ=60)
           GTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACG                                                                                    <  SRR4302540.3633734/94‑1 (MQ=60)
            TGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGGGGGGATCAATCTCGATCGTCATACGTTGGCGGGCATGCTAATACGT                                                                                   >  SRR4302540.4817834/1‑94 (MQ=60)
            TGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTACTACGTTGGCGGTCATGCTAATACGT                                                                                   >  SRR4302540.1653139/1‑94 (MQ=60)
            TGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGT                                                                                   >  SRR4302540.2033067/1‑94 (MQ=60)
             GTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTG                                                                                  >  SRR4302540.6432944/1‑94 (MQ=60)
              TTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGA                                                                                 <  SRR4302540.7013949/94‑1 (MQ=60)
              TTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGA                                                                                 >  SRR4302540.7860873/1‑94 (MQ=60)
               TGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAA                                                                                >  SRR4302540.5087013/1‑94 (MQ=60)
               TGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAA                                                                                <  SRR4302540.8412388/94‑1 (MQ=60)
                GAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAAT                                                                               >  SRR4302540.9026116/1‑94 (MQ=60)
                GAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAAT                                                                               >  SRR4302540.743402/1‑94 (MQ=60)
                   GAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTAC                                                                            >  SRR4302540.9301362/1‑94 (MQ=60)
                   GAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTAC                                                                            <  SRR4302540.6813416/94‑1 (MQ=60)
                   GAGAGTATCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTAC                                                                            <  SRR4302540.2159421/94‑1 (MQ=60)
                    AGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACG                                                                           <  SRR4302540.1242802/94‑1 (MQ=60)
                    AGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACG                                                                           <  SRR4302540.8159777/94‑1 (MQ=60)
                     GAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGT                                                                          >  SRR4302540.9263500/1‑94 (MQ=60)
                     GAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGT                                                                          >  SRR4302540.8535568/1‑94 (MQ=60)
                     GAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGT                                                                          >  SRR4302540.8535567/1‑94 (MQ=60)
                     GAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGT                                                                          <  SRR4302540.4783925/94‑1 (MQ=60)
                     GAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGT                                                                          <  SRR4302540.4841236/94‑1 (MQ=60)
                      AGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTG                                                                         >  SRR4302540.9378353/1‑94 (MQ=60)
                      AGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTG                                                                         >  SRR4302540.4368767/1‑94 (MQ=60)
                      AGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTG                                                                         <  SRR4302540.2544340/94‑1 (MQ=60)
                       GTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAGGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATTCTAATACGTGAATTACGTGC                                                                        <  SRR4302540.198030/94‑1 (MQ=60)
                       GTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGC                                                                        >  SRR4302540.7711307/1‑94 (MQ=60)
                       GTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGC                                                                        >  SRR4302540.5286309/1‑94 (MQ=60)
                       GTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGC                                                                        <  SRR4302540.198029/94‑1 (MQ=60)
                       GTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGC                                                                        >  SRR4302540.6443892/1‑94 (MQ=60)
                       GTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTGGGCGGTCATGCTAATACGTGAATTACGTGC                                                                        >  SRR4302540.217510/1‑94 (MQ=60)
                        TGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT                                                                       >  SRR4302540.6753613/1‑94 (MQ=60)
                         GTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑G                                                                     >  SRR4302540.438425/1‑94 (MQ=60)
                         GTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑G                                                                     <  SRR4302540.9248869/94‑1 (MQ=60)
                          TCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GC                                                                    <  SRR4302540.9867190/94‑1 (MQ=60)
                            GTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGT                                                                  >  SRR4302540.8158472/1‑94 (MQ=60)
                             TTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTT                                                                 <  SRR4302540.2763147/94‑1 (MQ=60)
                             TTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTT                                                                 >  SRR4302540.5162165/1‑94 (MQ=60)
                             TTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTT                                                                 >  SRR4302540.4773220/1‑94 (MQ=60)
                              TAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTG                                                                <  SRR4302540.5969787/94‑1 (MQ=60)
                               AATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGG                                                               <  SRR4302540.8046620/94‑1 (MQ=60)
                               AATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATATCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGG                                                               <  SRR4302540.5729466/94‑1 (MQ=60)
                                ATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCTTGCTAATACGTGAATTAAGTGCT‑GCGGTGGA                                                              >  SRR4302540.7504387/1‑94 (MQ=60)
                                ATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGA                                                              <  SRR4302540.7638357/94‑1 (MQ=60)
                                ATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGA                                                              >  SRR4302540.7239979/1‑94 (MQ=60)
                                ATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACCTGAATTACGTGCT‑GCGTTGGA                                                              >  SRR4302540.8457135/1‑94 (MQ=60)
                                ATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGA                                                              >  SRR4302540.7339644/1‑94 (MQ=60)
                                 TCAGGGGTGGATCACGCTGGAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAA                                                             <  SRR4302540.8164489/94‑1 (MQ=60)
                                 TCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAA                                                             >  SRR4302540.3980933/1‑94 (MQ=60)
                                  CAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAAC                                                            <  SRR4302540.7846629/94‑1 (MQ=60)
                                  CAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAAC                                                            <  SRR4302540.3690326/94‑1 (MQ=60)
                                  CAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAAC                                                            >  SRR4302540.5000898/1‑94 (MQ=60)
                                   atggggtGATCACGCTGCATGAAGGGGGGATCAATCTCGATCGTAATACGTTGGCGGGCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACT                                                           <  SRR4302540.2399815/87‑1 (MQ=60)
                                   AGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACT                                                           >  SRR4302540.1595529/1‑94 (MQ=60)
                                   AGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACT                                                           >  SRR4302540.2610491/1‑94 (MQ=60)
                                   AGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACT                                                           >  SRR4302540.7953511/1‑94 (MQ=60)
                                    GGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTC                                                          <  SRR4302540.7058512/94‑1 (MQ=60)
                                     GGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCT                                                         >  SRR4302540.9482576/1‑94 (MQ=60)
                                     GGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCT                                                         <  SRR4302540.5081282/94‑1 (MQ=60)
                                     GGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATAGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCT                                                         <  SRR4302540.660724/94‑1 (MQ=60)
                                      GGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTT                                                        <  SRR4302540.4889925/94‑1 (MQ=60)
                                      GGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTT                                                        <  SRR4302540.6204825/94‑1 (MQ=60)
                                      GGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTT                                                        >  SRR4302540.7122377/1‑94 (MQ=60)
                                       GTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCC‑GCGTTGGAACTCTTC                                                       >  SRR4302540.4334386/1‑94 (MQ=60)
                                        TGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCG                                                      <  SRR4302540.8729874/94‑1 (MQ=60)
                                        TGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCG                                                      <  SRR4302540.7335536/94‑1 (MQ=60)
                                        TGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCG                                                      <  SRR4302540.4121016/94‑1 (MQ=60)
                                          GATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAA                                                    >  SRR4302540.6951240/1‑94 (MQ=60)
                                          GATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAA                                                    <  SRR4302540.7955950/94‑1 (MQ=60)
                                          GATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAA                                                    >  SRR4302540.5251553/1‑94 (MQ=60)
                                           ATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAAC                                                   >  SRR4302540.6075472/1‑94 (MQ=60)
                                            TCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACA                                                  >  SRR4302540.6788107/1‑94 (MQ=60)
                                            TCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACA                                                  >  SRR4302540.314520/1‑94 (MQ=60)
                                             CACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAA                                                 <  SRR4302540.5464084/94‑1 (MQ=60)
                                              ACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAG                                                >  SRR4302540.2204864/1‑94 (MQ=60)
                                                GCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAA                                              >  SRR4302540.3511123/1‑94 (MQ=60)
                                                GCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAA                                              <  SRR4302540.4479992/94‑1 (MQ=60)
                                                GCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAA‑TACGTGCTGGCGTTGGACCTTTTC‑AACAAGAAG                                             >  SRR4302540.8682134/1‑94 (MQ=60)
                                                 CTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAG                                             <  SRR4302540.2692099/94‑1 (MQ=60)
                                                 CTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAG                                             <  SRR4302540.464195/94‑1 (MQ=60)
                                                  TGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGCACTCTTCGAACAAGAAGG                                            >  SRR4302540.5707103/1‑94 (MQ=60)
                                                  TGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGG                                            >  SRR4302540.5825183/1‑94 (MQ=60)
                                                  TGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGG                                            <  SRR4302540.4425051/94‑1 (MQ=60)
                                                  TGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGG                                            >  SRR4302540.2387219/1‑94 (MQ=60)
                                                    CAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGAT                                          >  SRR4302540.9649768/1‑94 (MQ=60)
                                                     AGGAAGCGGGGTTCAATCTCTATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTGCGAACAAGAAGGATT                                         >  SRR4302540.9058190/1‑94 (MQ=60)
                                                     AGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATT                                         <  SRR4302540.3591333/94‑1 (MQ=60)
                                                     AGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATT                                         >  SRR4302540.4099919/1‑94 (MQ=60)
                                                      GGAAGCGTGGATCAATCTCGATCGTAATACGTTGTCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTG                                        <  SRR4302540.7176638/94‑1 (MQ=60)
                                                      GGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTG                                        >  SRR4302540.7174982/1‑94 (MQ=60)
                                                      GGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTG                                        >  SRR4302540.2371180/1‑94 (MQ=60)
                                                       GAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGG                                       >  SRR4302540.6933232/1‑94 (MQ=60)
                                                        AAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGC                                      >  SRR4302540.1616552/1‑94 (MQ=60)
                                                        AAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGC                                      <  SRR4302540.6563973/94‑1 (MQ=60)
                                                        AAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGC                                      >  SRR4302540.1616553/1‑94 (MQ=60)
                                                            GGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCT                                  >  SRR4302540.3162265/1‑94 (MQ=60)
                                                            GGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCT                                  >  SRR4302540.6073403/1‑94 (MQ=60)
                                                             GGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTT                                 <  SRR4302540.9042989/94‑1 (MQ=60)
                                                             GGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTT                                 <  SRR4302540.425362/94‑1 (MQ=60)
                                                             GGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTT                                 <  SRR4302540.1201197/94‑1 (MQ=60)
                                                              GGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTA                                <  SRR4302540.6000757/94‑1 (MQ=60)
                                                              GGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTA                                >  SRR4302540.8273613/1‑94 (MQ=60)
                                                               GATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAT                               <  SRR4302540.4820692/94‑1 (MQ=60)
                                                               GATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAT                               >  SRR4302540.2042285/1‑94 (MQ=60)
                                                               GATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAT                               >  SRR4302540.3266089/1‑94 (MQ=60)
                                                               GATCAATCTCGATCGTAAGTCGTTGGCGCTCATGCTAATACGTTAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAT                               <  SRR4302540.2822256/94‑1 (MQ=60)
                                                               GATCAATCTCGATCGCAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAT                               <  SRR4302540.1562827/94‑1 (MQ=60)
                                                                ATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATC                              >  SRR4302540.9402752/1‑94 (MQ=60)
                                                                ATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATC                              >  SRR4302540.6033656/1‑94 (MQ=60)
                                                                 TCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACCAGAAGGATTGGCACCTTATCT                             >  SRR4302540.6074900/1‑94 (MQ=60)
                                                                 TCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCT                             >  SRR4302540.5458650/1‑94 (MQ=60)
                                                                 TCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCT                             >  SRR4302540.10112270/1‑94 (MQ=60)
                                                                 TCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCT                             <  SRR4302540.6041774/94‑1 (MQ=60)
                                                                  CAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTG                            <  SRR4302540.9810817/94‑1 (MQ=60)
                                                                  CAATCTCGATCGTAATACGTTGGCGGTCATGCGAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATCGGCACCTTATCGG                            >  SRR4302540.7812343/1‑94 (MQ=60)
                                                                   AATCTCTATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGT                           >  SRR4302540.7447716/1‑94 (MQ=60)
                                                                   AATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGT                           >  SRR4302540.7992710/1‑94 (MQ=60)
                                                                   AATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGT                           <  SRR4302540.7231610/94‑1 (MQ=60)
                                                                   AATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGT                           <  SRR4302540.8357410/94‑1 (MQ=60)
                                                                   AATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGT                           >  SRR4302540.3164367/1‑94 (MQ=60)
                                                                    ATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTC                          >  SRR4302540.9634002/1‑94 (MQ=60)
                                                                    ATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTC                          >  SRR4302540.2882855/1‑94 (MQ=60)
                                                                    ATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTC                          >  SRR4302540.4776786/1‑94 (MQ=60)
                                                                    ATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTC                          <  SRR4302540.2529048/94‑1 (MQ=60)
                                                                     TCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCG                         >  SRR4302540.9531962/1‑94 (MQ=60)
                                                                     TCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCG                         <  SRR4302540.1816540/94‑1 (MQ=60)
                                                                      CTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGC                        >  SRR4302540.650646/1‑94 (MQ=60)
                                                                      CTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGC                        >  SRR4302540.9714326/1‑94 (MQ=60)
                                                                      CTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGC                        >  SRR4302540.2121662/1‑94 (MQ=60)
                                                                        CGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGC                      >  SRR4302540.6584101/1‑94 (MQ=60)
                                                                        CGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGC                      <  SRR4302540.534013/94‑1 (MQ=60)
                                                                        CGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGC                      >  SRR4302540.9018964/1‑94 (MQ=60)
                                                                         GATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCT                     >  SRR4302540.5707287/1‑94 (MQ=60)
                                                                         GATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCT                     <  SRR4302540.5523506/94‑1 (MQ=60)
                                                                         GATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCT                     >  SRR4302540.515824/1‑94 (MQ=60)
                                                                          ATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTTGCGCTG                    >  SRR4302540.4220369/1‑94 (MQ=60)
                                                                          ATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTG                    <  SRR4302540.7860293/94‑1 (MQ=60)
                                                                          ATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTG                    >  SRR4302540.3441596/1‑94 (MQ=60)
                                                                          ATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTG                    >  SRR4302540.6858998/1‑94 (MQ=60)
                                                                           TCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGG                   >  SRR4302540.5196799/1‑94 (MQ=60)
                                                                           TCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGATCTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGG                   <  SRR4302540.700852/94‑1 (MQ=60)
                                                                            CGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGG                  >  SRR4302540.3693774/1‑94 (MQ=60)
                                                                              TAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAA                >  SRR4302540.3211367/1‑94 (MQ=60)
                                                                              TAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAA                >  SRR4302540.7262631/1‑94 (MQ=60)
                                                                              TAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAA                >  SRR4302540.7054258/1‑94 (MQ=60)
                                                                               AATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAA               >  SRR4302540.6859272/1‑94 (MQ=60)
                                                                                ATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAA              <  SRR4302540.637226/94‑1 (MQ=60)
                                                                                ATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAA              >  SRR4302540.6854434/1‑94 (MQ=60)
                                                                                ATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAA              >  SRR4302540.4554823/1‑94 (MQ=60)
                                                                                ATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAA              >  SRR4302540.3370365/1‑94 (MQ=60)
                                                                                 TACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAG             <  SRR4302540.9059303/94‑1 (MQ=60)
                                                                                 TACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAG             <  SRR4302540.8985015/94‑1 (MQ=60)
                                                                                 TACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAG             >  SRR4302540.8019411/1‑94 (MQ=60)
                                                                                  ACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGC            <  SRR4302540.321141/94‑1 (MQ=60)
                                                                                  ACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGC            >  SRR4302540.8331207/1‑94 (MQ=60)
                                                                                  ACGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGC            <  SRR4302540.7282163/94‑1 (MQ=60)
                                                                                   CGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCCCCTTATCTGTCGCGCTGGGAAAAGCT           <  SRR4302540.2102145/94‑1 (MQ=60)
                                                                                   CGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCT           <  SRR4302540.318105/94‑1 (MQ=60)
                                                                                   CGTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCT           <  SRR4302540.6502640/94‑1 (MQ=60)
                                                                                    GTTGGTGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGAATGGCACCTTATCTGTCGCGCTGGGAAAAGCTG          >  SRR4302540.1282559/1‑94 (MQ=60)
                                                                                    GTTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTG          >  SRR4302540.6182994/1‑94 (MQ=60)
                                                                                    GTGGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCGGTCGCGCTGGGAAAAGCTG          <  SRR4302540.9602106/94‑1 (MQ=60)
                                                                                     TTGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGG         >  SRR4302540.9762442/1‑94 (MQ=60)
                                                                                      TGGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGA        >  SRR4302540.7072908/1‑94 (MQ=60)
                                                                                       GGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGAT       >  SRR4302540.8920014/1‑94 (MQ=60)
                                                                                       GGCGGTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGAT       >  SRR4302540.8920013/1‑94 (MQ=60)
                                                                                        GCGGGCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATA      <  SRR4302540.5585093/94‑1 (MQ=60)
                                                                                         CGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAA     <  SRR4302540.9248092/94‑1 (MQ=60)
                                                                                           GTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCGCCTTATCTGTCGCGCTGGGAAAAGCTGGATAATT   >  SRR4302540.6282661/1‑94 (MQ=60)
                                                                                           GTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATT   <  SRR4302540.1750889/94‑1 (MQ=60)
                                                                                           GTCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATT   >  SRR4302540.2153002/1‑94 (MQ=60)
                                                                                            TCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTT  >  SRR4302540.4846972/1‑94 (MQ=60)
                                                                                            TCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTT  <  SRR4302540.1771421/94‑1 (MQ=60)
                                                                                            |                                                                                              
GGCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCT‑GCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTT  >  NC_000913/4171287‑4171472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑