Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,006,4801.C97.4% 1567.2 / 25.0 417coding (186/207 nt)b4554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (4/7);  new base C (221/184);  total (226/191)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 482 total aligned reads displayed.

AGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  NC_000913/4006385‑4006574
                                                                                                 |                                                                                              
aGCGGCTCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑Gt                                                                                                 <  1:6459867/96‑1 (MQ=255)
aGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑Gt                                                                                                 >  2:3061881/1‑96 (MQ=255)
aGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑Gt                                                                                                 >  2:5721837/1‑96 (MQ=255)
  cggcgCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cc                                                                                              <  1:7480686/96‑1 (MQ=255)
   gtcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccc                                                                                             >  1:8659421/3‑96 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACCCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            <  2:3224062/96‑2 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            <  2:11090180/96‑2 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            <  2:9345158/96‑2 (MQ=255)
    gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                            >  1:14397567/1‑95 (MQ=255)
      gcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccccaa                                                                                          <  2:8375762/96‑4 (MQ=255)
       cgcCCCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCCAcc                                                                                         <  1:10660948/96‑1 (MQ=255)
       cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccccaac                                                                                         <  2:9821362/96‑5 (MQ=255)
         ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCCAaccc                                                                                       >  2:11716443/1‑96 (MQ=255)
         ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCCAaccc                                                                                       <  1:13958909/96‑1 (MQ=255)
          cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                       >  2:5213073/1‑96 (MQ=255)
          cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                       >  1:5523796/1‑96 (MQ=255)
          cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                       >  2:7938292/1‑96 (MQ=255)
           aCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCa                                                                                      >  2:5464347/1‑96 (MQ=255)
           aCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCa                                                                                      <  1:9422235/96‑1 (MQ=255)
              gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAAGAGC‑GTCCCCAACCCCACGc                                                                                   >  1:12386419/1‑96 (MQ=255)
               ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGcc                                                                                  <  1:11978323/96‑1 (MQ=255)
                tgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGGC‑CCCAACCCCACGCCa                                                                                 <  2:12904732/96‑1 (MQ=255)
                 ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCaa                                                                                <  2:8893347/96‑1 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               <  2:6250215/96‑1 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               >  2:13967218/1‑96 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               <  1:12241910/96‑1 (MQ=255)
                  gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAc                                                                               >  1:2627806/1‑96 (MQ=255)
                   aTTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAAcc                                                                              <  2:10713742/96‑1 (MQ=255)
                    ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCa                                                                             <  2:4855692/96‑1 (MQ=255)
                     tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAg                                                                            <  1:2279730/96‑1 (MQ=255)
                     tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAg                                                                            <  2:1442041/96‑1 (MQ=255)
                      cccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGc                                                                           >  1:13598709/1‑96 (MQ=255)
                       ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGGC‑CCCAACCCCACGCCAACCAGca                                                                          >  1:169484/1‑96 (MQ=255)
                        cAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGcac                                                                         >  1:10299841/1‑96 (MQ=255)
                        cAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTGCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGcac                                                                         >  1:12988872/1‑96 (MQ=255)
                          gCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACaa                                                                       >  1:5657304/1‑96 (MQ=255)
                            cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAata                                                                     >  1:4690884/1‑96 (MQ=255)
                            cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAata                                                                     >  1:5840720/1‑96 (MQ=255)
                              aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATg                                                                   >  2:7605486/1‑96 (MQ=255)
                              aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATg                                                                   <  1:11650757/96‑1 (MQ=255)
                                 cAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                                <  1:1975576/96‑1 (MQ=255)
                                  aaGTAAGCCGCGCCTGCCCCCTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATc                                                               <  2:169817/96‑1 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGACTCg                                                              <  2:12576085/96‑1 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                              <  2:1813590/96‑1 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                              >  2:7203147/1‑96 (MQ=255)
                                   aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                              >  1:10034643/1‑96 (MQ=255)
                                     tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                            <  1:9155985/96‑1 (MQ=255)
                                      aaGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCa                                                           <  2:13733318/96‑1 (MQ=255)
                                       aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAg                                                          >  2:3687372/1‑96 (MQ=255)
                                        gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                         <  2:338295/96‑1 (MQ=255)
                                        gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                         <  1:9704987/96‑1 (MQ=255)
                                        gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                         <  1:7782484/96‑1 (MQ=255)
                                          cgcgCCTGCCCACTTCAACACTTAAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                       <  1:2980693/96‑1 (MQ=255)
                                           gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                                      <  2:3970458/96‑1 (MQ=255)
                                            cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                                     <  1:10484123/96‑1 (MQ=255)
                                            cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                                     >  1:7986817/1‑96 (MQ=255)
                                             gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGGACAATATGAATCGCCAGTCCGGt                                                    >  1:10128629/1‑96 (MQ=255)
                                               cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                                  >  1:6892320/1‑96 (MQ=255)
                                                tGCCCACTTCCACACTTCAAACGCAATCCCTGAGCGGGAAAATAGC‑GTCCCCACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                                 <  1:4068998/96‑1 (MQ=255)
                                                tGCCCACTTCAACCCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                                 <  2:3502030/96‑1 (MQ=255)
                                                tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                                 >  2:1215072/1‑96 (MQ=255)
                                                 gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCGACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                                <  1:3111070/96‑1 (MQ=255)
                                                 gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                                >  2:6570231/1‑96 (MQ=255)
                                                  cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                               <  1:3463851/96‑1 (MQ=255)
                                                   ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAg                                              >  2:1338418/1‑96 (MQ=255)
                                                     actccaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGcc                                            <  2:2566037/96‑1 (MQ=255)
                                                      cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCATCCCCACGCGAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                           <  1:12603412/96‑1 (MQ=255)
                                                      cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                           >  2:13046441/1‑96 (MQ=255)
                                                        tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                         <  2:1941456/96‑1 (MQ=255)
                                                         cgacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGc                                        >  1:11102629/3‑96 (MQ=255)
                                                         caacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGc                                        <  2:2209687/96‑1 (MQ=255)
                                                          aacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                       >  1:13377356/1‑96 (MQ=255)
                                                          aacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                       >  2:1054826/1‑96 (MQ=255)
                                                          aacactccaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                       <  2:9637291/96‑1 (MQ=255)
                                                             acttcaaACGCAATCACCGAGCGGGAAAATAGC‑GTCCCCACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaaca                                    <  2:8153106/96‑3 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGGAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                >  1:12861599/1‑96 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                <  1:14159854/96‑1 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                >  2:11414924/1‑96 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                <  2:4180760/96‑1 (MQ=255)
                                                                 caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                                <  2:450918/96‑1 (MQ=255)
                                                                  aaaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGc                               <  2:10195636/96‑1 (MQ=255)
                                                                   aaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                              <  1:7507994/96‑1 (MQ=255)
                                                                    aCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCa                             >  1:4587549/1‑96 (MQ=255)
                                                                     cGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                            <  2:8659421/96‑1 (MQ=255)
                                                                      gCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                           <  1:9591017/96‑1 (MQ=255)
                                                                      gCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                           >  2:5995745/1‑96 (MQ=255)
                                                                        aaTCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgc                         <  1:3801520/96‑1 (MQ=255)
                                                                          tCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgc                       >  1:3825965/1‑96 (MQ=255)
                                                                           ctcTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGGAATAGACGCCAccgcgcc                      >  1:11782086/3‑96 (MQ=255)
                                                                            aCTGAGTGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg                     <  1:5145082/96‑1 (MQ=255)
                                                                            aCTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg                     >  2:12086768/1‑96 (MQ=255)
                                                                            aCTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg                     <  1:8572703/96‑1 (MQ=255)
                                                                              tGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAACCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcg                   <  2:2694721/96‑1 (MQ=255)
                                                                                 gCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa                <  1:8218582/96‑1 (MQ=255)
                                                                                   gggAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc              >  1:4340198/1‑96 (MQ=255)
                                                                                     gAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCg            >  1:2195964/1‑96 (MQ=255)
                                                                                      aaaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt           >  2:7021968/1‑96 (MQ=255)
                                                                                       aaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg          <  2:10036187/96‑1 (MQ=255)
                                                                                          tAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGtt       <  1:8934487/96‑1 (MQ=255)
                                                                                          tAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGtt       >  2:9501562/1‑96 (MQ=255)
                                                                                                 |                                                                                              
AGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  NC_000913/4006385‑4006574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 289 total aligned reads displayed.
CCAGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCA  >  NC_000913/4006383‑4006583
                                                                                                         |                                                                                                     
 NNNCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑C‑C                                                                                                     <  SRR4302518.8936842/101‑1 (MQ=60)
 CAGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑N‑N‑N                                                                                                     >  SRR4302518.13522627/1‑101 (MQ=60)
 CAGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑N‑N‑N                                                                                                     >  SRR4302518.11596166/1‑101 (MQ=60)
  AGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑N‑NN                                                                                                    >  SRR4302518.10439860/1‑101 (MQ=60)
  AGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑C‑CA                                                                                                    >  SRR4302518.6587344/1‑101 (MQ=60)
    NNNCGCGCCCCTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑C‑CACC                                                                                                  <  SRR4302518.11161959/101‑1 (MQ=60)
    NNNCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AAC                                                                                                   <  SRR4302518.12470936/101‑1 (MQ=60)
    CGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AAC                                                                                                   >  SRR4302518.5740227/1‑101 (MQ=60)
     GTCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑Ccannn                                                                                                  >  SRR4302518.9080793/1‑96 (MQ=60)
      GCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCC                                                                                                 >  SRR4302518.4384111/1‑101 (MQ=60)
      GCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑Ccaannn                                                                                                 >  SRR4302518.15065612/1‑95 (MQ=60)
       NNNGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCC                                                                                                <  SRR4302518.14252129/101‑1 (MQ=60)
        NNNCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCA                                                                                               <  SRR4302518.9877933/101‑1 (MQ=60)
        GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCA                                                                                               <  SRR4302518.5083456/101‑1 (MQ=60)
        GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑Ccaaccnnn                                                                                               >  SRR4302518.10013343/1‑93 (MQ=60)
        GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑Ccaaccnnn                                                                                               >  SRR4302518.10004532/1‑93 (MQ=60)
        GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑Ccaaccccc                                                                                               >  SRR4302518.3221891/1‑93 (MQ=60)
         CGCCACTGCTGGATTCCCAGCCAAATCAAGTAATACGCGACTGCCCACTTCAACACTTCAAACGCAATCACTGAGAGGGAAAAGAGA‑G‑T‑C‑C‑C‑AAaacacac                                                                                             >  SRR4302518.3566343/1‑94 (MQ=60)
         CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCAC                                                                                              <  SRR4302518.4916935/101‑1 (MQ=60)
         CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑Ccaacccnnn                                                                                              >  SRR4302518.13436173/1‑92 (MQ=60)
          GCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCNNN                                                                                             >  SRR4302518.14701563/1‑101 (MQ=60)
          GCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCNNN                                                                                             >  SRR4302518.8754133/1‑101 (MQ=60)
          GCCACCCCTGCATTCCCAGCTAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATCGC‑G‑TCC‑C‑C‑AACCCCACG                                                                                             <  SRR4302518.5690757/101‑1 (MQ=60)
            gacgGCTGGATTCCCAGCCAAACCAAGTAAGCCGCGCCTCCCCACTTCAACACTTAAAACGCAATCACTGAGCGGGAAAATACC‑G‑T‑C‑C‑Cgacgcccaccac                                                                                           >  SRR4302518.4497990/5‑89 (MQ=60)
            NNNTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCC                                                                                           <  SRR4302518.12549588/101‑1 (MQ=60)
            CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTAAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACNNN                                                                                           >  SRR4302518.9150926/1‑101 (MQ=60)
             NNNGCTGGATTCCCAGCCAAATCAAGTAGCCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCA                                                                                          <  SRR4302518.13168511/101‑1 (MQ=60)
             NNNGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCA                                                                                          <  SRR4302518.9223981/101‑1 (MQ=60)
              CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCNNN                                                                                         >  SRR4302518.9399209/1‑101 (MQ=60)
               TGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACCCTTCAAACGCAAACACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAAC                                                                                        <  SRR4302518.34243/101‑1 (MQ=60)
               TGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAAC                                                                                        <  SRR4302518.803985/101‑1 (MQ=60)
               NNNTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCTACGCCAAC                                                                                        <  SRR4302518.13590653/101‑1 (MQ=60)
               NNNTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAAC                                                                                        <  SRR4302518.12821578/101‑1 (MQ=60)
                GCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAAGAGC‑G‑TCC‑C‑C‑AACCCCACGCCANNN                                                                                       >  SRR4302518.12970480/1‑101 (MQ=60)
                 nnngatcccccgcacAATCAAGTAAGCCGCGCCTGCCCTATTCAACACTTCAAACGCAAACACTGAGCGGGAAAATAGC‑G‑A‑C‑CGC‑AACCCCACGCCAACCA                                                                                      <  SRR4302518.14874598/86‑1 (MQ=60)
                  TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAG                                                                                     >  SRR4302518.2029839/1‑101 (MQ=60)
                  TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAG                                                                                     <  SRR4302518.2390375/101‑1 (MQ=60)
                    GCTTCCCAGCCAAACCAAGAAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   <  SRR4302518.6157486/101‑1 (MQ=60)
                    GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   >  SRR4302518.4160093/1‑101 (MQ=60)
                    GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   >  SRR4302518.2753551/1‑101 (MQ=60)
                    GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   >  SRR4302518.6837392/1‑101 (MQ=60)
                    GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   >  SRR4302518.6874021/1‑101 (MQ=60)
                    GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   >  SRR4302518.4071099/1‑101 (MQ=60)
                    GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCA                                                                                   >  SRR4302518.3384346/1‑101 (MQ=60)
                     ATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAAacacnnn                                                                                  >  SRR4302518.10712256/1‑94 (MQ=60)
                      TTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATATC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCNNN                                                                                 >  SRR4302518.10549538/1‑101 (MQ=60)
                       TCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAA                                                                                >  SRR4302518.39731/1‑101 (MQ=60)
                       TCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGAGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCANNN                                                                                >  SRR4302518.10512614/1‑101 (MQ=60)
                       NNNCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAA                                                                                <  SRR4302518.13090931/101‑1 (MQ=60)
                        CGCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACNNN                                                                               >  SRR4302518.9862753/1‑101 (MQ=60)
                         CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGG‑C‑C‑C‑C‑AACCCCACGCCAACCAGCACAACA                                                                              >  SRR4302518.176386/1‑101 (MQ=60)
                         CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCgcannn                                                                              >  SRR4302518.7890652/1‑95 (MQ=60)
                         CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATA                                                                              >  SRR4302518.1703012/1‑101 (MQ=60)
                          CAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAANNN                                                                             >  SRR4302518.10785513/1‑101 (MQ=60)
                          CAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTGCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAANNN                                                                             >  SRR4302518.13590729/1‑101 (MQ=60)
                          CAGCCAAATCAAGGAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATAT                                                                             >  SRR4302518.2316065/1‑101 (MQ=60)
                           NNNCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATG                                                                            <  SRR4302518.12199183/101‑1 (MQ=60)
                           CGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑ACCCCCCCGCCACCCAGCACAATATG                                                                            <  SRR4302518.6135463/101‑1 (MQ=60)
                           AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATG                                                                            >  SRR4302518.5064826/1‑101 (MQ=60)
                           AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑CTC‑C‑AACACCACGCAAACCAGCACAATATA                                                                            >  SRR4302518.3184353/1‑101 (MQ=60)
                            GCCAAATCAAGTAAGCCGCGGCTGCCCACTTCAACCCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGA                                                                           <  SRR4302518.1892167/101‑1 (MQ=60)
                            GCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGA                                                                           >  SRR4302518.5944508/1‑101 (MQ=60)
                             CCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAA                                                                          <  SRR4302518.3993022/101‑1 (MQ=60)
                              CAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAAT                                                                         >  SRR4302518.4937445/1‑101 (MQ=60)
                               AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGANNN                                                                        >  SRR4302518.8233336/1‑101 (MQ=60)
                                nnnaAAGTAAGCCGCGCCTGCCGACTTCAACCCTTCAAACGCAATCCCTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATAAGAATCG                                                                       <  SRR4302518.9420818/97‑1 (MQ=60)
                                CATCAAGTAAGCCGCGCCTGCCCACCTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCG                                                                       <  SRR4302518.4613485/101‑1 (MQ=60)
                                AATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCG                                                                       <  SRR4302518.1122572/101‑1 (MQ=60)
                                 ATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGC                                                                      >  SRR4302518.2600129/1‑101 (MQ=60)
                                  TCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCNNN                                                                     >  SRR4302518.12515804/1‑101 (MQ=60)
                                  NNNAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCC                                                                     <  SRR4302518.9597540/101‑1 (MQ=60)
                                     NNNAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AAGCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                                  <  SRR4302518.10905813/101‑1 (MQ=60)
                                     NNNAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                                  <  SRR4302518.14629817/101‑1 (MQ=60)
                                     NNNAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                                  <  SRR4302518.10171860/101‑1 (MQ=60)
                                     AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCTCCAGC                                                                  >  SRR4302518.7255461/1‑101 (MQ=60)
                                     AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCNNN                                                                  >  SRR4302518.14213205/1‑101 (MQ=60)
                                      NNNAGCTGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTC                                                                 <  SRR4302518.10882213/101‑1 (MQ=60)
                                      NNNAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTC                                                                 <  SRR4302518.12270811/101‑1 (MQ=60)
                                       TAAGGCGCGCCTGCCCACGTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGNNN                                                                >  SRR4302518.10642822/1‑101 (MQ=60)
                                       TAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                                >  SRR4302518.1724530/1‑101 (MQ=60)
                                        AAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                               >  SRR4302518.2287926/1‑101 (MQ=60)
                                         NNNCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                              <  SRR4302518.10974888/101‑1 (MQ=60)
                                         AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                              <  SRR4302518.3166925/101‑1 (MQ=60)
                                         AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                              <  SRR4302518.2127326/101‑1 (MQ=60)
                                          GCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCacgtcannn                                                             >  SRR4302518.9058427/1‑92 (MQ=60)
                                           CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGNNN                                                            >  SRR4302518.8428413/1‑101 (MQ=60)
                                           CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                            >  SRR4302518.1399652/1‑101 (MQ=60)
                                            CGCGCCTGCCCACTTCAACACTTCAAACGCAATCGCTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCGCTCCGGTCT                                                           >  SRR4302518.5411462/1‑101 (MQ=60)
                                             GCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AGCCCCACACCAACCAGCACAATATGAATCGCCAGTCCAGTCTG                                                          >  SRR4302518.211883/1‑101 (MQ=60)
                                             GCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTNNN                                                          >  SRR4302518.8628589/1‑101 (MQ=60)
                                             GCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTNNN                                                          >  SRR4302518.12742086/1‑101 (MQ=60)
                                             GCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTNNN                                                          >  SRR4302518.10408140/1‑101 (MQ=60)
                                              CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCGACCAGCACAATATGAATCGCCAGTCCGGTCTGA                                                         <  SRR4302518.3256974/101‑1 (MQ=60)
                                              CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTTCGGTCNNN                                                         >  SRR4302518.7887687/1‑101 (MQ=60)
                                              CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGA                                                         <  SRR4302518.2736978/101‑1 (MQ=60)
                                              CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCNNN                                                         >  SRR4302518.8385475/1‑101 (MQ=60)
                                               GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACATTATGAATCGCCTGTCGGGTGTGAA                                                        >  SRR4302518.7087454/1‑101 (MQ=60)
                                               GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                        >  SRR4302518.3901727/1‑101 (MQ=60)
                                               GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                        <  SRR4302518.3625060/101‑1 (MQ=60)
                                               GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                        >  SRR4302518.3400723/1‑101 (MQ=60)
                                               GCCTGCACACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTNNN                                                        >  SRR4302518.8814140/1‑101 (MQ=60)
                                                CCTGCCCACTTCAACTCTTCACACGCAATCACTGAGCGGCAACATAGC‑G‑TCC‑C‑C‑AACCCCACGCCATCCAGCACAATATGAATCGCCAGTCCGGTCTGAAG                                                       <  SRR4302518.1611094/101‑1 (MQ=60)
                                                CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAAGCAGCACAATATGAATCGCCAGTCCGGTCTGNNN                                                       >  SRR4302518.12823075/1‑101 (MQ=60)
                                                CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGagatcgga                                                       >  SRR4302518.4195817/1‑93 (MQ=60)
                                                CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGNNN                                                       >  SRR4302518.12916387/1‑101 (MQ=60)
                                                 CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGANNN                                                      >  SRR4302518.12092537/1‑101 (MQ=60)
                                                 CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGANNN                                                      >  SRR4302518.9492877/1‑101 (MQ=60)
                                                 CAGCCCACTTCAACACTTTAAACGCAATCACTGAGCGGGCAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGANNN                                                      >  SRR4302518.10631116/1‑101 (MQ=60)
                                                  CGCCCTCTTCACCACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAGCCAGCACAATATGAATCGCCAGccgcctgatcgtga                                                     <  SRR4302518.647935/101‑15 (MQ=60)
                                                   NNNCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑ATCCCCACGCGAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCC                                                    <  SRR4302518.13193838/101‑1 (MQ=60)
                                                   GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCGGGTCTGAAGNNN                                                    >  SRR4302518.14859917/1‑101 (MQ=60)
                                                     CCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCNNN                                                  >  SRR4302518.8794261/1‑101 (MQ=60)
                                                     CCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCNNN                                                  >  SRR4302518.8287549/1‑101 (MQ=60)
                                                     CCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAG                                                  <  SRR4302518.620871/101‑1 (MQ=60)
                                                      NNNTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGC                                                 <  SRR4302518.8379238/101‑1 (MQ=60)
                                                      CACTTCAACACTTCAAACGCAATGACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCNNN                                                 >  SRR4302518.9723567/1‑101 (MQ=60)
                                                        CTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGCATCGCCAGTCCGGTCTGAAGCCCAGNNN                                               >  SRR4302518.13148247/1‑101 (MQ=60)
                                                         TTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAT                                              <  SRR4302518.4902873/101‑1 (MQ=60)
                                                          TCACCCCTTCAAACGCAGTCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATA                                             <  SRR4302518.2330200/101‑1 (MQ=60)
                                                          TCAACACTTCAAACGCAATCACTGTGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCGACGCCAACCAGCACAATATGAATCACCAGTCCGGTCTGAAGCCCAGCANNN                                             >  SRR4302518.12506723/1‑101 (MQ=60)
                                                          TCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGatataaacaccagcaata                                             >  SRR4302518.297058/1‑83 (MQ=60)
                                                           NNNCACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATCG                                            <  SRR4302518.12042083/101‑1 (MQ=60)
                                                           CGACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAANNN                                            >  SRR4302518.11622023/1‑101 (MQ=60)
                                                           CGACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGGCTGAAGCCTAGCAANNN                                            >  SRR4302518.11343862/1‑101 (MQ=60)
                                                           CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGACGCCCAGCAANNN                                            >  SRR4302518.7601683/1‑101 (MQ=60)
                                                            AACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATNNN                                           >  SRR4302518.13992690/1‑101 (MQ=60)
                                                             NNNCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGCC                                          <  SRR4302518.11057635/101‑1 (MQ=60)
                                                             NNNCTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                          <  SRR4302518.13781661/101‑1 (MQ=60)
                                                              NNNTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                         <  SRR4302518.14809431/101‑1 (MQ=60)
                                                               ACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TGC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCATCAATAGACGC                                        >  SRR4302518.5878410/1‑101 (MQ=60)
                                                                NNNCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCC                                       <  SRR4302518.7893855/101‑1 (MQ=60)
                                                                CTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCC                                       <  SRR4302518.799083/101‑1 (MQ=60)
                                                                 TTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGNNN                                      >  SRR4302518.9217361/1‑101 (MQ=60)
                                                                 TTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCA                                      <  SRR4302518.6058577/101‑1 (MQ=60)
                                                                 NNNAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCTA                                      <  SRR4302518.11564261/101‑1 (MQ=60)
                                                                  TCAAACGCAATCACTCAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAC                                     <  SRR4302518.2775211/101‑1 (MQ=60)
                                                                   NNNACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                                    <  SRR4302518.10054493/101‑1 (MQ=60)
                                                                   CAAACGCAATCACTGAGCGGGGAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGcagccnnn                                    >  SRR4302518.13459201/1‑93 (MQ=60)
                                                                   CAAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                                    <  SRR4302518.4677824/101‑1 (MQ=60)
                                                                   CAAACGCAATCACGGAGCGGGAAAATAGC‑GCC‑C‑C‑C‑ACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                                    <  SRR4302518.6011966/101‑1 (MQ=60)
                                                                    NNNCGCGATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                                   <  SRR4302518.13501959/101‑1 (MQ=60)
                                                                    AAACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                                   <  SRR4302518.2266569/101‑1 (MQ=60)
                                                                     NNNGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCCCCGC                                  <  SRR4302518.14393178/101‑1 (MQ=60)
                                                                     AACGCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGC                                  <  SRR4302518.3980985/101‑1 (MQ=60)
                                                                      ACGCAATCACTAAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCAACCNNN                                 >  SRR4302518.12236623/1‑101 (MQ=60)
                                                                        NNNATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                               <  SRR4302518.9032902/101‑1 (MQ=60)
                                                                        NNNATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                               <  SRR4302518.14684686/101‑1 (MQ=60)
                                                                        GCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                               <  SRR4302518.4892511/101‑1 (MQ=60)
                                                                        GCAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AAACCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGGAATAGACGCCAACGCNNN                               >  SRR4302518.12929953/1‑101 (MQ=60)
                                                                        GCAATCACGGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCNNN                               >  SRR4302518.13573919/1‑101 (MQ=60)
                                                                         NNNTCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCG                              <  SRR4302518.8990662/101‑1 (MQ=60)
                                                                         CAATCACTGAGTGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCG                              <  SRR4302518.5410796/101‑1 (MQ=60)
                                                                         CAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCCCGCGAACCAGCACAAAATAACTGGCCAGTCCGGTCTGACGCCCAccacaacacgcacccgcgccg                              >  SRR4302518.4549130/1‑80 (MQ=32)
                                                                         CAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCCCGCCAACCAGCCCAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCG                              <  SRR4302518.1949379/101‑1 (MQ=60)
                                                                         CAATCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTTAAGCCCAGCAATAGACGCCACCGCGNNN                              >  SRR4302518.10554573/1‑101 (MQ=60)
                                                                            TCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGA                           <  SRR4302518.2384690/101‑1 (MQ=60)
                                                                            TCACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGA                           >  SRR4302518.4006910/1‑101 (MQ=60)
                                                                             CACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                          >  SRR4302518.579059/1‑101 (MQ=60)
                                                                              NNNGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                         <  SRR4302518.8624471/101‑1 (MQ=60)
                                                                              ACTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGNNN                         >  SRR4302518.14446482/1‑101 (MQ=60)
                                                                               CTGAGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAA                        >  SRR4302518.5851250/1‑101 (MQ=60)
                                                                                TGAGCGGTAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCTAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCCGCAATAGACGACCCCGCGCCGCGATAAC                       >  SRR4302518.6565466/1‑101 (MQ=60)
                                                                                 GTGCGGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATANNN                      >  SRR4302518.12314238/1‑101 (MQ=60)
                                                                                   nnnggcAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGT                    <  SRR4302518.14472759/95‑1 (MQ=60)
                                                                                    CGGGAAAATAGC‑G‑TCC‑C‑C‑ACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG                   <  SRR4302518.5032359/101‑1 (MQ=60)
                                                                                     NNNAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG                  <  SRR4302518.14179551/101‑1 (MQ=60)
                                                                                     GGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG                  >  SRR4302518.4557346/1‑101 (MQ=60)
                                                                                     GGGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGNNN                  >  SRR4302518.8501600/1‑101 (MQ=60)
                                                                                      GGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTNNN                 >  SRR4302518.12564384/1‑101 (MQ=60)
                                                                                      GGAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTNNN                 >  SRR4302518.10108347/1‑101 (MQ=60)
                                                                                       NNNAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTT                <  SRR4302518.9366956/101‑1 (MQ=60)
                                                                                       GAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCCCAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTT                <  SRR4302518.444500/101‑1 (MQ=60)
                                                                                       GAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGNNN                >  SRR4302518.10154176/1‑101 (MQ=60)
                                                                                       GAAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTT                >  SRR4302518.2301557/1‑101 (MQ=60)
                                                                                        nnnaaatagcttcC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGCT                 <  SRR4302518.11155270/88‑1 (MQ=60)
                                                                                        AAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTG               >  SRR4302518.4699734/1‑101 (MQ=60)
                                                                                        AAAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTG               >  SRR4302518.3087469/1‑101 (MQ=60)
                                                                                         AAATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTNNN              >  SRR4302518.13867392/1‑101 (MQ=60)
                                                                                          AGTAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTNNN             >  SRR4302518.11069298/1‑101 (MQ=60)
                                                                                          AATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCGAGCAATAGACGCCAACGCGCCGCGATAAGCGTGGTTNNN             >  SRR4302518.7670387/1‑101 (MQ=60)
                                                                                          AATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAG             >  SRR4302518.3810277/1‑101 (MQ=60)
                                                                                           ATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAGCCAGCACAATATGAATCGCCAGTCCGGACTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGC            <  SRR4302518.6058797/101‑1 (MQ=60)
                                                                                           ATAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGNNN            >  SRR4302518.7792710/1‑101 (MQ=60)
                                                                                            TAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG           >  SRR4302518.799926/1‑101 (MQ=60)
                                                                                            TAGC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAACCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG           <  SRR4302518.6133102/101‑1 (MQ=60)
                                                                                            NNNC‑G‑T‑C‑C‑CAAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGACACCGCGCCGCGATAACCGTGGTTGAGGG           <  SRR4302518.12654855/101‑1 (MQ=60)
                                                                                             nnngcgtcC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGCCGCCACCGCGCCGGGATAACCGTGGTTGATC            <  SRR4302518.10305563/93‑1 (MQ=60)
                                                                                              GC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGGCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGATGAGCNNN         >  SRR4302518.14181121/1‑101 (MQ=60)
                                                                                              GC‑G‑TCC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTACGGTCTGAAGCCCAGCAATAtacgcaaccacgccgtgaaaacccatgtagagtgag         >  SRR4302518.4326103/1‑65 (MQ=60)
                                                                                               N‑N‑N‑C‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCACAGCAATAGACGCCCCCGCGCCGCGATAACCGTGGTTGAGCGAGGT       <  SRR4302518.14633179/101‑1 (MQ=60)
                                                                                                 nnncC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGG        <  SRR4302518.14941260/97‑1 (MQ=60)
                                                                                                 N‑N‑N‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATCGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTG      <  SRR4302518.10657919/101‑1 (MQ=60)
                                                                                                  gtcC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGANNN       >  SRR4302518.14973935/4‑101 (MQ=60)
                                                                                                   tcC‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGGGGTG      >  SRR4302518.5826249/3‑101 (MQ=60)
                                                                                                   C‑C‑C‑C‑ACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGG     <  SRR4302518.939203/101‑1 (MQ=60)
                                                                                                     ctC‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGNNN     >  SRR4302518.8194867/3‑101 (MQ=60)
                                                                                                     cgC‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGACACCGCTCCGCGATAACCGTGGTTGAGCGAGGNNN     >  SRR4302518.10754974/3‑101 (MQ=60)
                                                                                                     C‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGT    >  SRR4302518.5800808/1‑101 (MQ=60)
                                                                                                     C‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGCGGT    >  SRR4302518.2486214/1‑101 (MQ=60)
                                                                                                         C‑ACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCA  <  SRR4302518.1553082/101‑1 (MQ=60)
                                                                                                         |                                                                                                     
CCAGCGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑G‑T‑C‑C‑C‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCA  >  NC_000913/4006383‑4006583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑