Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,006,4801.C98.6% 660.8 / 1.5 209coding (186/207 nt)b4554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (121/85);  total (123/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 233 total aligned reads displayed.

GGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006388‑4006567
                                                                                             |                                                                                       
ggcgCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGt                                                                                          <  1:6090170/93‑1 (MQ=255)
ggcgCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGt                                                                                          <  2:1097265/93‑1 (MQ=255)
   gcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccc                                                                                      >  1:2229395/1‑93 (MQ=255)
    cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccc                                                                                     >  2:603873/1‑92 (MQ=255)
    cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccc                                                                                     <  2:5856681/93‑2 (MQ=255)
     gcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccca                                                                                    >  1:5837088/1‑91 (MQ=255)
      ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaa                                                                                   <  1:3009666/93‑4 (MQ=255)
       cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaac                                                                                  >  1:4125987/1‑89 (MQ=255)
         ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCCAaccc                                                                                <  1:3623994/93‑1 (MQ=255)
         ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCCAaccc                                                                                >  1:4936111/1‑93 (MQ=255)
         ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCCAaccc                                                                                >  2:5698688/1‑93 (MQ=255)
            ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCAc                                                                              <  1:3608523/93‑1 (MQ=255)
             tgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACg                                                                             <  2:1518409/93‑1 (MQ=255)
              ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGc                                                                            >  2:1117417/1‑93 (MQ=255)
               gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGcc                                                                           >  1:4580952/1‑93 (MQ=255)
               gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGcc                                                                           >  2:160707/1‑93 (MQ=255)
                 ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCaa                                                                         <  2:1550896/93‑1 (MQ=255)
                 ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCaa                                                                         >  1:3127188/1‑93 (MQ=255)
                  tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAAc                                                                        <  1:3713869/93‑1 (MQ=255)
                    ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCa                                                                      >  1:1795623/1‑93 (MQ=255)
                      aGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGc                                                                    <  1:2467660/93‑1 (MQ=255)
                      aGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGc                                                                    <  1:3365690/93‑1 (MQ=255)
                         cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGcaca                                                                 >  2:466010/1‑93 (MQ=255)
                         cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGcaca                                                                 >  2:5446395/1‑93 (MQ=255)
                          aaaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACaa                                                                >  2:5941988/1‑93 (MQ=255)
                          aaaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACaa                                                                <  1:714517/93‑1 (MQ=255)
                            aTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAata                                                              <  2:795312/93‑1 (MQ=255)
                             tCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAatat                                                             <  2:5310474/93‑1 (MQ=255)
                             tCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAatat                                                             >  1:2322579/1‑93 (MQ=255)
                              cAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATg                                                            >  1:5804883/1‑93 (MQ=255)
                               aaGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGa                                                           <  1:4830845/93‑1 (MQ=255)
                                aGTAAGCCGGGCCTGCCCACTTCAACAATTCAAACGCAATCACTGAGCCGGAAAATAGCGTCCCCAACCCCACGCCACCCAGCACAATATGaa                                                          <  1:3463978/93‑1 (MQ=255)
                                aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGaa                                                          <  1:1218787/93‑1 (MQ=255)
                                aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGaa                                                          <  2:2663275/93‑1 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                         >  2:1561341/1‑93 (MQ=255)
                                  tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATc                                                        <  2:4778785/93‑1 (MQ=255)
                                    aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGc                                                      >  1:795882/1‑93 (MQ=255)
                                     gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                     >  1:2711024/1‑93 (MQ=255)
                                     gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                     <  1:1802694/93‑1 (MQ=255)
                                      ccGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCa                                                    >  1:3424449/1‑93 (MQ=255)
                                       cgcgCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAg                                                   >  2:4042146/1‑93 (MQ=255)
                                         cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                 <  2:4599788/93‑1 (MQ=255)
                                         cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                 <  2:3831685/93‑1 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                <  2:4885833/93‑1 (MQ=255)
                                           ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               <  1:6222220/93‑1 (MQ=255)
                                            cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              >  1:4006076/1‑93 (MQ=255)
                                             tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                             >  2:4846759/1‑93 (MQ=255)
                                             tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                             >  2:2231650/1‑93 (MQ=255)
                                             tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                             >  2:5085574/1‑93 (MQ=255)
                                             tGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                             >  2:23914/1‑93 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            >  1:902688/1‑93 (MQ=255)
                                               cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           >  1:6163770/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  1:3795264/1‑93 (MQ=255)
                                                 cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         >  1:5046223/1‑93 (MQ=255)
                                                 cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         >  1:5504425/1‑93 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        <  2:4148717/93‑1 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  2:4405322/1‑93 (MQ=255)
                                                    ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      >  1:2160179/1‑93 (MQ=255)
                                                    ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      >  1:5472596/1‑93 (MQ=255)
                                                     tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGTCAGTCCGGTCTGAAGcc                                     <  1:4498817/93‑1 (MQ=255)
                                                       aacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCa                                   <  2:464024/93‑1 (MQ=255)
                                                        acacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                  >  1:5909138/1‑93 (MQ=255)
                                                        acacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCAAGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                  >  1:4257200/1‑93 (MQ=255)
                                                          acttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAATCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAACa                                >  2:2036045/1‑93 (MQ=255)
                                                          acttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                >  2:4448601/1‑93 (MQ=255)
                                                           cttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaa                               <  2:3537750/93‑1 (MQ=255)
                                                           cttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaa                               >  2:3231709/1‑93 (MQ=255)
                                                           cttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaa                               >  2:2964284/1‑93 (MQ=255)
                                                              caaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAg                            >  1:1147538/1‑93 (MQ=255)
                                                               aaaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           <  2:691524/93‑1 (MQ=255)
                                                               aaaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           <  2:2824750/93‑1 (MQ=255)
                                                               aaaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           <  1:5221963/93‑1 (MQ=255)
                                                                aaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                          >  2:3494475/1‑93 (MQ=255)
                                                                  cGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGc                        <  2:2087279/93‑1 (MQ=255)
                                                                   gCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       >  1:1670701/1‑93 (MQ=255)
                                                                    cAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCa                      >  1:2974199/1‑93 (MQ=255)
                                                                    cAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCa                      <  1:1013363/93‑1 (MQ=255)
                                                                     aaTCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                     <  1:3668318/93‑1 (MQ=255)
                                                                      aTCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    >  1:1282579/1‑93 (MQ=255)
                                                                      aTCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    >  1:2731456/1‑93 (MQ=255)
                                                                       tCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                   >  1:2038391/1‑93 (MQ=255)
                                                                       tCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                   >  1:1961628/1‑93 (MQ=255)
                                                                        cACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGTCAccgc                  <  2:5242158/93‑1 (MQ=255)
                                                                          cTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGTCAccgcgc                <  2:6149457/93‑1 (MQ=255)
                                                                          cTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgc                <  2:3658048/93‑1 (MQ=255)
                                                                           tGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               >  1:2758338/1‑93 (MQ=255)
                                                                           tGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               >  1:1568287/1‑93 (MQ=255)
                                                                           tGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               <  2:5502212/93‑1 (MQ=255)
                                                                             aGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgc             >  2:1060119/1‑93 (MQ=255)
                                                                               cGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGa           >  2:3120994/1‑93 (MQ=255)
                                                                               cGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGa           >  1:5981351/1‑93 (MQ=255)
                                                                                gggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAt          >  1:824841/1‑93 (MQ=255)
                                                                                 ggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         >  1:4325864/1‑93 (MQ=255)
                                                                                 ggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         >  1:5104931/1‑93 (MQ=255)
                                                                                   aaaaTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc       >  1:5266389/1‑93 (MQ=255)
                                                                                    aaaTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc      >  1:4466950/1‑93 (MQ=255)
                                                                                      aTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    <  1:1606922/93‑1 (MQ=255)
                                                                                      aTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    <  2:1410040/93‑1 (MQ=255)
                                                                                      aTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    <  2:4022724/93‑1 (MQ=255)
                                                                                       tAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTg   >  1:1401731/1‑93 (MQ=255)
                                                                                             |                                                                                       
GGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006388‑4006567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  NC_000913/4006390‑4006574
                                                                                              |                                                                                            
CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC                                                                                              >  SRR4302533.2243155/1‑94 (MQ=60)
  CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑CA                                                                                           >  SRR4302533.4767460/1‑94 (MQ=60)
  CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑CA                                                                                           >  SRR4302533.5872382/1‑94 (MQ=60)
  CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑CA                                                                                           >  SRR4302533.2763758/1‑94 (MQ=60)
   GCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCcaa                                                                                           <  SRR4302533.1748194/94‑4 (MQ=60)
   GCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCcaa                                                                                           >  SRR4302533.626504/1‑91 (MQ=60)
    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAC                                                                                          >  SRR4302533.4151032/1‑94 (MQ=60)
    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AAC                                                                                          <  SRR4302533.3028081/94‑1 (MQ=60)
     CATTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACC                                                                                         <  SRR4302533.2203528/94‑1 (MQ=60)
     CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACC                                                                                         >  SRR4302533.2083009/1‑94 (MQ=60)
     CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACC                                                                                         >  SRR4302533.3007331/1‑94 (MQ=60)
      ACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCC                                                                                        >  SRR4302533.4965998/1‑94 (MQ=60)
       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                       <  SRR4302533.3646295/94‑1 (MQ=60)
       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                       >  SRR4302533.5407999/1‑94 (MQ=60)
       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                       >  SRR4302533.410368/1‑94 (MQ=60)
       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCC                                                                                       >  SRR4302533.239467/1‑94 (MQ=60)
          CTGGATTCCCGGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACG                                                                                    <  SRR4302533.3630738/94‑1 (MQ=60)
          CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACG                                                                                    >  SRR4302533.4477095/1‑94 (MQ=60)
          CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACG                                                                                    <  SRR4302533.3630737/94‑1 (MQ=60)
           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                   <  SRR4302533.4900954/94‑1 (MQ=60)
           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                   >  SRR4302533.2261427/1‑94 (MQ=60)
           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                   <  SRR4302533.2237079/94‑1 (MQ=60)
           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                   >  SRR4302533.1052821/1‑94 (MQ=60)
           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGC                                                                                   >  SRR4302533.6117036/1‑94 (MQ=60)
            GGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCC                                                                                  >  SRR4302533.4608711/1‑94 (MQ=60)
             GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCA                                                                                 >  SRR4302533.1315251/1‑94 (MQ=60)
             GATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCA                                                                                 >  SRR4302533.5777234/1‑94 (MQ=60)
              ATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAA                                                                                >  SRR4302533.3146412/1‑94 (MQ=60)
              ATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAA                                                                                >  SRR4302533.5846854/1‑94 (MQ=60)
                TCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACC                                                                              <  SRR4302533.3736671/94‑1 (MQ=60)
                 CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGCAAATAGCGTCCCC‑AACCCCACGACAACCA                                                                             >  SRR4302533.4989729/1‑94 (MQ=60)
                 CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCA                                                                             >  SRR4302533.1806551/1‑94 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCA                                                                          <  SRR4302533.3045513/94‑1 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCA                                                                          <  SRR4302533.2482880/94‑1 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCA                                                                          >  SRR4302533.5081937/1‑94 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCA                                                                          <  SRR4302533.3386479/94‑1 (MQ=60)
                      CCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACA                                                                        >  SRR4302533.4379650/1‑94 (MQ=60)
                      CCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACA                                                                        <  SRR4302533.5218350/94‑1 (MQ=60)
                        AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAAT                                                                      >  SRR4302533.2915893/1‑94 (MQ=60)
                        AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAAT                                                                      <  SRR4302533.718733/94‑1 (MQ=60)
                          ATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATAT                                                                    <  SRR4302533.755601/94‑1 (MQ=60)
                          ATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATAT                                                                    >  SRR4302533.2336952/1‑94 (MQ=60)
                           TCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATG                                                                   >  SRR4302533.5839938/1‑94 (MQ=60)
                            CAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGA                                                                  <  SRR4302533.5851327/94‑1 (MQ=60)
                             AAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAA                                                                 >  SRR4302533.902470/1‑94 (MQ=60)
                             AAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAA                                                                 <  SRR4302533.4860123/94‑1 (MQ=60)
                             AAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAA                                                                 >  SRR4302533.2047397/1‑94 (MQ=60)
                              AGTAAGCCGGGCCTGCCCACTTCAACAATTCAAACGCAATCACTGAGCCGGAAAATAGCGTCCCC‑AACCCCACGCCACCCAGCACAATATGAAT                                                                <  SRR4302533.3485362/94‑1 (MQ=60)
                              AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAAT                                                                <  SRR4302533.1226105/94‑1 (MQ=60)
                                TAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCG                                                              <  SRR4302533.4341993/94‑1 (MQ=60)
                                 AAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGC                                                             >  SRR4302533.800564/1‑94 (MQ=60)
                                  AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCC                                                            >  SRR4302533.2693268/1‑94 (MQ=60)
                                  AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCC                                                            >  SRR4302533.2727665/1‑94 (MQ=60)
                                  AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCC                                                            <  SRR4302533.3718322/94‑1 (MQ=60)
                                   GCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCA                                                           <  SRR4302533.1813666/94‑1 (MQ=60)
                                   GCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCA                                                           >  SRR4302533.3445605/1‑94 (MQ=60)
                                    CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAG                                                          >  SRR4302533.6322945/1‑94 (MQ=60)
                                     CGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                         >  SRR4302533.1595881/1‑94 (MQ=60)
                                      GCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTC                                                        >  SRR4302533.1807939/1‑94 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                       <  SRR4302533.1631830/94‑1 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGACC                                                       >  SRR4302533.2056277/1‑94 (MQ=60)
                                        GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                      <  SRR4302533.5341981/94‑1 (MQ=60)
                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                     >  SRR4302533.3000451/1‑94 (MQ=60)
                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                     <  SRR4302533.6259513/94‑1 (MQ=60)
                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                     >  SRR4302533.4030465/1‑94 (MQ=60)
                                          CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGT                                                    >  SRR4302533.3581019/1‑94 (MQ=60)
                                          CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGT                                                    >  SRR4302533.5901525/1‑94 (MQ=60)
                                          CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGT                                                    <  SRR4302533.5965148/94‑1 (MQ=60)
                                          CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGT                                                    <  SRR4302533.822807/94‑1 (MQ=60)
                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                   <  SRR4302533.1887939/94‑1 (MQ=60)
                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                   >  SRR4302533.907983/1‑94 (MQ=60)
                                            GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                  >  SRR4302533.5519099/1‑94 (MQ=60)
                                            GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                  >  SRR4302533.6200826/1‑94 (MQ=60)
                                             CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTG                                                 >  SRR4302533.3818491/1‑94 (MQ=60)
                                              CCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGA                                                >  SRR4302533.5076750/1‑94 (MQ=60)
                                              CCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGA                                                >  SRR4302533.5537637/1‑94 (MQ=60)
                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCCCGCCAACCAGCACAATATAAATCGCCAGTCCGGTCTGAA                                               <  SRR4302533.4143784/94‑1 (MQ=60)
                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                               <  SRR4302533.935745/94‑1 (MQ=60)
                                                 CTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGC                                             >  SRR4302533.454241/1‑94 (MQ=60)
                                                 CTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGC                                             >  SRR4302533.2173477/1‑94 (MQ=60)
                                                 CTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGC                                             >  SRR4302533.5505577/1‑94 (MQ=60)
                                                 CGTCAACACTTCAAACGCAATCACTGAGCGGGAAAAGAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGC                                             <  SRR4302533.1913092/94‑1 (MQ=60)
                                                  TTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCC                                            >  SRR4302533.2741067/1‑94 (MQ=60)
                                                   TCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGTCAGTCCGGTCTGAAGCCC                                           <  SRR4302533.4526061/94‑1 (MQ=60)
                                                     AACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAG                                         >  SRR4302533.5944835/1‑94 (MQ=60)
                                                     AACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCAAGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAG                                         >  SRR4302533.4282865/1‑94 (MQ=60)
                                                         CTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAT                                     >  SRR4302533.1405637/1‑94 (MQ=60)
                                                         CTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAT                                     >  SRR4302533.3562806/1‑94 (MQ=60)
                                                          TTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATA                                    >  SRR4302533.1676381/1‑94 (MQ=60)
                                                           TCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAG                                   >  SRR4302533.1154386/1‑94 (MQ=60)
                                                            CAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                  <  SRR4302533.6224687/94‑1 (MQ=60)
                                                            CAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                  >  SRR4302533.4212629/1‑94 (MQ=60)
                                                            CAAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                  <  SRR4302533.2856215/94‑1 (MQ=60)
                                                             AAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                 >  SRR4302533.5290269/1‑94 (MQ=60)
                                                             AAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                 <  SRR4302533.5253384/94‑1 (MQ=60)
                                                             AAACGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                 <  SRR4302533.6082496/94‑1 (MQ=60)
                                                             AAACGCAATCACAGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                 <  SRR4302533.1553673/94‑1 (MQ=60)
                                                                CGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCC                              >  SRR4302533.1680800/1‑94 (MQ=60)
                                                                CGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCC                              >  SRR4302533.3669311/1‑94 (MQ=60)
                                                                CGCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGAAATAGACGCC                              >  SRR4302533.4495267/1‑94 (MQ=60)
                                                                 GCAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCA                             >  SRR4302533.2992398/1‑94 (MQ=60)
                                                                  CAATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAC                            <  SRR4302533.1019298/94‑1 (MQ=60)
                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                           <  SRR4302533.842426/94‑1 (MQ=60)
                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                           >  SRR4302533.2748217/1‑94 (MQ=60)
                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                           <  SRR4302533.3690863/94‑1 (MQ=60)
                                                                   AATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                           >  SRR4302533.1290298/1‑94 (MQ=60)
                                                                    ATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                          >  SRR4302533.2050964/1‑94 (MQ=60)
                                                                    ATCACTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                          >  SRR4302533.1973601/1‑94 (MQ=60)
                                                                        CTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                      >  SRR4302533.1577729/1‑94 (MQ=60)
                                                                        CTGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                      >  SRR4302533.2775247/1‑94 (MQ=60)
                                                                         TGAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCG                     >  SRR4302533.3316193/1‑94 (MQ=60)
                                                                          GAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGC                    >  SRR4302533.1218258/1‑94 (MQ=60)
                                                                          GAGCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGC                    >  SRR4302533.2192392/1‑94 (MQ=60)
                                                                           AGCGGGAAAATAGCGT‑CCCGAACCCCACGCCAACCAGCACAAGATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                   <  SRR4302533.1654243/94‑1 (MQ=60)
                                                                            GCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGA                  >  SRR4302533.6017576/1‑94 (MQ=60)
                                                                            GCGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGA                  >  SRR4302533.252441/1‑94 (MQ=60)
                                                                             CGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCTAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                 <  SRR4302533.3400154/94‑1 (MQ=60)
                                                                             CGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                 >  SRR4302533.3467487/1‑94 (MQ=60)
                                                                             CGGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                 >  SRR4302533.829681/1‑94 (MQ=60)
                                                                              GGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                >  SRR4302533.5135769/1‑94 (MQ=60)
                                                                              GGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                >  SRR4302533.4100499/1‑94 (MQ=60)
                                                                              GGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                >  SRR4302533.4351924/1‑94 (MQ=60)
                                                                              GGGAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                >  SRR4302533.3268037/1‑94 (MQ=60)
                                                                                GAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC              >  SRR4302533.5297989/1‑94 (MQ=60)
                                                                                GAAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC              <  SRR4302533.1277483/94‑1 (MQ=60)
                                                                                 AAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACC             >  SRR4302533.4493965/1‑94 (MQ=60)
                                                                                 AAAATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACC             >  SRR4302533.4838949/1‑94 (MQ=60)
                                                                                    ATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG          <  SRR4302533.1616673/94‑1 (MQ=60)
                                                                                    ATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG          >  SRR4302533.1410183/1‑94 (MQ=60)
                                                                                    ATAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG          <  SRR4302533.3733148/94‑1 (MQ=60)
                                                                                     TAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG         >  SRR4302533.3753954/1‑94 (MQ=60)
                                                                                     TAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG         >  SRR4302533.5112926/1‑94 (MQ=60)
                                                                                     TAGCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG         <  SRR4302533.3884442/94‑1 (MQ=60)
                                                                                       GCGTCCCC‑ACCCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTT       <  SRR4302533.2269643/94‑1 (MQ=60)
                                                                                       GCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTT       >  SRR4302533.5093539/1‑94 (MQ=60)
                                                                                       GCGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTT       <  SRR4302533.1370648/94‑1 (MQ=60)
                                                                                        CGTCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTG      <  SRR4302533.1337413/94‑1 (MQ=60)
                                                                                         gtcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA     >  SRR4302533.2622573/4‑94 (MQ=60)
                                                                                         gtcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA     >  SRR4302533.2787188/4‑94 (MQ=60)
                                                                                         gtcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA     >  SRR4302533.4151637/4‑94 (MQ=60)
                                                                                         gtcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA     >  SRR4302533.1005093/4‑94 (MQ=60)
                                                                                          tcCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAG    <  SRR4302533.4630835/92‑1 (MQ=60)
                                                                                          C‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCGGGTCTGACGCCCAGCAATAGACGacacagcgcagcgacagccggggatgagt   >  SRR4302533.1965168/1‑65 (MQ=60)
                                                                                          C‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGC   >  SRR4302533.3612986/1‑94 (MQ=60)
                                                                                            CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  SRR4302533.2533573/1‑94 (MQ=60)
                                                                                              |                                                                                            
CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG  >  NC_000913/4006390‑4006574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑