Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	747,952	A→G	V85A (GTA→GCA)	ybgO ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	747,952	0	A	G	100.0%	740.3 / NA	230	V85A (GTA→GCA)	ybgO	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (114/116); total (114/116)

Only 100 of 235 total aligned reads displayed.

CGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATC  >  NC_000913/747862‑748041

  aCTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCa                                                                                       <  1:6178505/93‑1 (MQ=255)

   cTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAg                                                                                      <  1:3571210/93‑1 (MQ=255)

    tttttCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAgg                                                                                     >  1:2106364/1‑93 (MQ=255)

      tttCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCg                                                                                   >  2:5996728/1‑93 (MQ=255)

      tttCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCg                                                                                   >  1:885243/1‑93 (MQ=255)

       ttCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGt                                                                                  >  1:6109655/1‑93 (MQ=255)

         cccAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAg                                                                                >  1:4274264/1‑93 (MQ=255)

         cccAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAg                                                                                <  2:3555632/93‑1 (MQ=255)

             gACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACAt                                                                            >  1:1042491/1‑93 (MQ=255)

             gACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGGTTGCCCAGGCGGAGACAt                                                                            >  2:4662806/1‑93 (MQ=255)

               cTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGc                                                                          <  2:3960434/93‑1 (MQ=255)

                 ggCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTc                                                                        >  2:710370/1‑93 (MQ=255)

                   cATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGc                                                                      <  1:3699437/93‑1 (MQ=255)

                    aTCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCt                                                                     <  1:597843/93‑1 (MQ=255)

                    aTCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCt                                                                     >  2:5025203/1‑93 (MQ=255)

                    aTCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCt                                                                     <  1:6272082/93‑1 (MQ=255)

                     tCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCtt                                                                    >  1:433852/1‑93 (MQ=255)

                      cGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCttt                                                                   >  2:3864117/1‑93 (MQ=255)

                        tAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCa                                                                 >  1:3112853/1‑93 (MQ=255)

                        tAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCa                                                                 <  2:3747333/93‑1 (MQ=255)

                         aaTCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCaa                                                                >  2:5054906/1‑93 (MQ=255)

                           tCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAg                                                              <  1:5737348/93‑1 (MQ=255)

                             gACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGtt                                                            >  1:3759754/1‑93 (MQ=255)

                                acCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                                                         <  2:3622285/93‑1 (MQ=255)

                                acCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                                                         >  2:1787511/1‑93 (MQ=255)

                                acCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                                                         >  1:1770386/1‑93 (MQ=255)

                                acCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                                                         >  2:584518/1‑93 (MQ=255)

                                   gTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGtt                                                      >  2:2329427/1‑93 (MQ=255)

                                    ttGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTg                                                     >  1:2724883/1‑93 (MQ=255)

                                    ttGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTg                                                     <  1:5294740/93‑1 (MQ=255)

                                     tGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTgg                                                    >  2:1682587/1‑93 (MQ=255)

                                     tGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTgg                                                    <  1:1656324/93‑1 (MQ=255)

                                      gTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGt                                                   <  2:4461008/93‑1 (MQ=255)

                                      gTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGt                                                   <  2:4533749/93‑1 (MQ=255)

                                       tATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTa                                                  >  1:4167723/1‑93 (MQ=255)

                                        aTGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAt                                                 <  1:4007291/93‑1 (MQ=255)

                                            cACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAttgtt                                             <  2:4882462/93‑1 (MQ=255)

                                            cACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAttgtt                                             <  2:2875753/93‑1 (MQ=255)

                                             aCGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAttgttg                                            >  1:3439788/1‑93 (MQ=255)

                                              cGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATtgttgt                                           >  2:6273354/1‑93 (MQ=255)

                                                ccGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTca                                         <  1:2135466/93‑1 (MQ=255)

                                                 cGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTcac                                        >  2:1576459/1‑93 (MQ=255)

                                                  gAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTcaca                                       >  2:4419653/1‑93 (MQ=255)

                                                  gAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTcaca                                       >  2:3690243/1‑93 (MQ=255)

                                                   aGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACaa                                      <  1:1844492/93‑1 (MQ=255)

                                                     ttGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATa                                    <  2:5283260/93‑1 (MQ=255)

                                                      tGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAg                                   <  2:258969/93‑1 (MQ=255)

                                                       gATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGa                                  <  2:5919030/93‑1 (MQ=255)

                                                       gATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGa                                  <  1:5232950/93‑1 (MQ=255)

                                                        ataataACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAc                                 >  2:3391725/1‑93 (MQ=255)

                                                        ataataACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAc                                 <  2:3245821/93‑1 (MQ=255)

                                                        ataataACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAc                                 >  2:4005238/1‑93 (MQ=255)

                                                        ataataACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAc                                 >  2:2229561/1‑93 (MQ=255)

                                                           ataaCGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACggg                              <  2:6211586/93‑1 (MQ=255)

                                                             aaCGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGaa                            <  1:4665204/93‑1 (MQ=255)

                                                             aaCGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGaa                            >  1:2961613/1‑93 (MQ=255)

                                                              aCGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAt                           <  2:2841776/93‑1 (MQ=255)

                                                              aCGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAt                           <  1:5815778/93‑1 (MQ=255)

                                                              aCGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAt                           <  2:522055/93‑1 (MQ=255)

                                                               cGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAtt                          >  2:2200521/1‑93 (MQ=255)

                                                                gATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAttt                         >  2:5740263/1‑93 (MQ=255)

                                                                gATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGCAAttt                         >  1:4989700/1‑93 (MQ=255)

                                                                 aTCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAtttt                        <  2:200693/93‑1 (MQ=255)

                                                                 aTCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAtttt                        <  2:2632479/93‑1 (MQ=255)

                                                                 aTCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAtttt                        >  2:1652273/1‑93 (MQ=255)

                                                                  tCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTa                       >  2:3903455/1‑93 (MQ=255)

                                                                  tCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTa                       >  2:6253690/1‑93 (MQ=255)

                                                                    cTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAat                     >  1:1170341/1‑93 (MQ=255)

                                                                    cTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAat                     >  2:2420346/1‑93 (MQ=255)

                                                                     tGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAata                    >  1:5423560/1‑93 (MQ=255)

                                                                     tGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAata                    <  2:4368492/93‑1 (MQ=255)

                                                                     tGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAata                    >  2:5085118/1‑93 (MQ=255)

                                                                     tGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGGCACAATAGACGGGAATTTTAata                    >  2:4576798/1‑93 (MQ=255)

                                                                      gCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAatat                   <  1:1364349/93‑1 (MQ=255)

                                                                          ccccGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTc               <  2:4546994/93‑1 (MQ=255)

                                                                           cccGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCa              <  2:3016867/93‑1 (MQ=255)

                                                                            ccGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg             >  2:6069196/1‑93 (MQ=255)

                                                                            ccGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg             <  1:3987926/93‑1 (MQ=255)

                                                                            ccGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg             <  1:52521/93‑1 (MQ=255)

                                                                             cGGATAGGGGTTGGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGa            <  1:1453618/93‑1 (MQ=255)

                                                                              ggATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAt           >  2:3288863/1‑93 (MQ=255)

                                                                              ggATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAt           >  1:5602145/1‑93 (MQ=255)

                                                                               gATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAtt          >  2:1415351/1‑93 (MQ=255)

                                                                               gATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAtt          <  1:2533292/93‑1 (MQ=255)

                                                                                aTAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTc         <  1:4759268/93‑1 (MQ=255)

                                                                                aTAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTc         <  1:2909138/93‑1 (MQ=255)

                                                                                aTAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTc         >  2:1013540/1‑93 (MQ=255)

                                                                                 tAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTcc        >  1:5342127/1‑93 (MQ=255)

                                                                                  aGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTccc       >  2:478621/1‑93 (MQ=255)

                                                                                  aGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTccc       <  2:2415539/93‑1 (MQ=255)

                                                                                   ggggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa      <  1:5100069/93‑1 (MQ=255)

                                                                                   ggggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa      >  2:5684114/1‑93 (MQ=255)

                                                                                   ggggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa      >  1:3351452/1‑93 (MQ=255)

                                                                                   ggggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa      >  1:2454519/1‑93 (MQ=255)

                                                                                   ggggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCa      >  2:379148/1‑93 (MQ=255)

                                                                                    gggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAg     <  1:672630/93‑1 (MQ=255)

                                                                                    gggTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAg     <  2:3039822/93‑1 (MQ=255)

                                                                                      gTTTGCCCAGGCGTAGACATGCTCACTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGAt   <  2:1932854/93‑1 (MQ=255)

                                                                                       tttGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATc  >  1:406013/1‑93 (MQ=255)

                                                                                       tttGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATc  <  2:659/93‑1 (MQ=255)

CGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATC  >  NC_000913/747862‑748041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTAT  >  NC_000913/747859‑748045

ATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTG                                                                                               <  SRR4302533.196932/94‑1 (MQ=60)

ATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTG                                                                                               <  SRR4302533.5114574/94‑1 (MQ=60)

 TGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGC                                                                                              <  SRR4302533.6043351/94‑1 (MQ=60)

 TGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGC                                                                                              >  SRR4302533.2735838/1‑94 (MQ=60)

    GACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCA                                                                                           >  SRR4302533.4181385/1‑94 (MQ=60)

     ACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAG                                                                                          >  SRR4302533.5136880/1‑94 (MQ=60)

     ACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAG                                                                                          <  SRR4302533.6215616/94‑1 (MQ=60)

      CTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGG                                                                                         >  SRR4302533.2119353/1‑94 (MQ=60)

      CTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGG                                                                                         <  SRR4302533.3593218/94‑1 (MQ=60)

        TTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCG                                                                                       <  SRR4302533.1757353/94‑1 (MQ=60)

        TTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCG                                                                                       <  SRR4302533.4015241/94‑1 (MQ=60)

        TTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCG                                                                                       >  SRR4302533.890444/1‑94 (MQ=60)

         TTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGT                                                                                      >  SRR4302533.6146538/1‑94 (MQ=60)

           TCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAG                                                                                    >  SRR4302533.4299980/1‑94 (MQ=60)

            CCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGC                                                                                   >  SRR4302533.1850410/1‑94 (MQ=60)

              CAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACA                                                                                 <  SRR4302533.5675950/94‑1 (MQ=60)

               AGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACAT                                                                                <  SRR4302533.5975099/94‑1 (MQ=60)

               AGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACAT                                                                                >  SRR4302533.1048620/1‑94 (MQ=60)

                GACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCCCCCCCGGATAGGGGTTTGCCCAGGCGTAGACATG                                                                               >  SRR4302533.1812498/1‑94 (MQ=60)

                 ACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGC                                                                              <  SRR4302533.282352/94‑1 (MQ=60)

                  CTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCT                                                                             >  SRR4302533.5300434/1‑94 (MQ=60)

                   TGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTC                                                                            <  SRR4302533.2559034/94‑1 (MQ=60)

                   TGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTC                                                                            <  SRR4302533.1436343/94‑1 (MQ=60)

                      CATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCT                                                                         <  SRR4302533.3722158/94‑1 (MQ=60)

                      CATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCT                                                                         >  SRR4302533.3468927/1‑94 (MQ=60)

                       ATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTT                                                                        <  SRR4302533.6309546/94‑1 (MQ=60)

                       ATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTT                                                                        <  SRR4302533.601366/94‑1 (MQ=60)

                       ATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTT                                                                        >  SRR4302533.436438/1‑94 (MQ=60)

                       ATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTT                                                                        >  SRR4302533.5372693/1‑94 (MQ=60)

                         CGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTC                                                                      <  SRR4302533.3766977/94‑1 (MQ=60)

                          GTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCA                                                                     >  SRR4302533.3131997/1‑94 (MQ=60)

                          GTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCA                                                                     <  SRR4302533.3790887/94‑1 (MQ=60)

                              TCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGT                                                                 <  SRR4302533.5771976/94‑1 (MQ=60)

                               CGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTT                                                                >  SRR4302533.3782802/1‑94 (MQ=60)

                               CGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTT                                                                >  SRR4302533.5281322/1‑94 (MQ=60)

                                 ACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGC                                                              >  SRR4302533.1614936/1‑94 (MQ=60)

                                 ACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGC                                                              >  SRR4302533.2146111/1‑94 (MQ=60)

                                  CACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCC                                                             >  SRR4302533.1781166/1‑94 (MQ=60)

                                  CACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCC                                                             <  SRR4302533.84808/94‑1 (MQ=60)

                                      GTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTG                                                         >  SRR4302533.362097/1‑94 (MQ=60)

                                      GTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTG                                                         >  SRR4302533.2741603/1‑94 (MQ=60)

                                       TTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGG                                                        <  SRR4302533.5326451/94‑1 (MQ=60)

                                        TGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGT                                                       >  SRR4302533.2891785/1‑94 (MQ=60)

                                        TGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGT                                                       <  SRR4302533.1435173/94‑1 (MQ=60)

                                        TGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGT                                                       <  SRR4302533.1666347/94‑1 (MQ=60)

                                         GTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTA                                                      >  SRR4302533.4192980/1‑94 (MQ=60)

                                           ATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATT                                                    <  SRR4302533.4031689/94‑1 (MQ=60)

                                              TCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTT                                                 <  SRR4302533.2951452/94‑1 (MQ=60)

                                               CACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTT                                                >  SRR4302533.5058386/1‑94 (MQ=60)

                                               CACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTG                                                >  SRR4302533.3461032/1‑94 (MQ=60)

                                               CACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTG                                                <  SRR4302533.5063234/94‑1 (MQ=60)

                                                   CCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCAC                                            <  SRR4302533.2148632/94‑1 (MQ=60)

                                                   CCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCAC                                            <  SRR4302533.5795800/94‑1 (MQ=60)

                                                    CGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACA                                           <  SRR4302533.6173118/94‑1 (MQ=60)

                                                      AGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAAT                                         <  SRR4302533.1855741/94‑1 (MQ=60)

                                                       GTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATA                                        <  SRR4302533.5556358/94‑1 (MQ=60)

                                                        TTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAG                                       <  SRR4302533.2477147/94‑1 (MQ=60)

                                                         TGATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGA                                      <  SRR4302533.1162400/94‑1 (MQ=60)

                                                         TGATAATAACGATCCGGCACCCGCGGATAGAGGTTTGCTCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGA                                      <  SRR4302533.3036181/94‑1 (MQ=60)

                                                          GATAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAC                                     <  SRR4302533.5264422/94‑1 (MQ=60)

                                                            TAATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGG                                   <  SRR4302533.1272991/94‑1 (MQ=60)

                                                             AATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGG                                  <  SRR4302533.4399769/94‑1 (MQ=60)

                                                              ATAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGA                                 >  SRR4302533.641427/1‑94 (MQ=60)

                                                               TAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAA                                >  SRR4302533.3374874/1‑94 (MQ=60)

                                                               TAACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAA                                >  SRR4302533.2979716/1‑94 (MQ=60)

                                                                AACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAT                               <  SRR4302533.3678994/94‑1 (MQ=60)

                                                                AACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAT                               <  SRR4302533.4693468/94‑1 (MQ=60)

                                                                 ACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATT                              >  SRR4302533.2361775/1‑94 (MQ=60)

                                                                 ACGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATT                              <  SRR4302533.5850903/94‑1 (MQ=60)

                                                                  CGATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGCAATTT                             >  SRR4302533.5019951/1‑94 (MQ=60)

                                                                   GATCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTT                            >  SRR4302533.4345483/1‑94 (MQ=60)

                                                                     TCCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAA                          >  SRR4302533.4719488/1‑94 (MQ=60)

                                                                      CCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAAT                         >  SRR4302533.315148/1‑94 (MQ=60)

                                                                      CCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAAT                         >  SRR4302533.882871/1‑94 (MQ=60)

                                                                      CCTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAAT                         >  SRR4302533.1177368/1‑94 (MQ=60)

                                                                       CTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATA                        >  SRR4302533.5456225/1‑94 (MQ=60)

                                                                       CTGCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATA                        >  SRR4302533.4213005/1‑94 (MQ=60)

                                                                         GCACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATC                      <  SRR4302533.1372546/94‑1 (MQ=60)

                                                                          CACCCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCG                     >  SRR4302533.5878670/1‑94 (MQ=60)

                                                                             CCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCA                  >  SRR4302533.4928161/1‑94 (MQ=60)

                                                                             CCCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCA                  <  SRR4302533.2328784/94‑1 (MQ=60)

                                                                              CCCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAG                 <  SRR4302533.2106899/94‑1 (MQ=60)

                                                                               CCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGA                <  SRR4302533.52877/94‑1 (MQ=60)

                                                                               CCGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGA                <  SRR4302533.4012218/94‑1 (MQ=60)

                                                                                CGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAT               <  SRR4302533.5377229/94‑1 (MQ=60)

                                                                                CGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAT               >  SRR4302533.5635997/1‑94 (MQ=60)

                                                                                CGGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAT               >  SRR4302533.3909721/1‑94 (MQ=60)

                                                                                CGGATAGGGGTTGGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGAT               <  SRR4302533.1462385/94‑1 (MQ=60)

                                                                                 GGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATT              >  SRR4302533.4052445/1‑94 (MQ=60)

                                                                                 GGATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATT              <  SRR4302533.3268660/94‑1 (MQ=60)

                                                                                  GATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTC             <  SRR4302533.2548886/94‑1 (MQ=60)

                                                                                  GATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTC             <  SRR4302533.4422352/94‑1 (MQ=60)

                                                                                  GATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTC             <  SRR4302533.6276974/94‑1 (MQ=60)

                                                                                   ATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCC            <  SRR4302533.2926996/94‑1 (MQ=60)

                                                                                   ATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCC            >  SRR4302533.5374164/1‑94 (MQ=60)

                                                                                   ATAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCC            <  SRR4302533.4788139/94‑1 (MQ=60)

                                                                                    TAGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTCGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCC           >  SRR4302533.6101277/1‑94 (MQ=60)

                                                                                     AGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCA          >  SRR4302533.2469668/1‑94 (MQ=60)

                                                                                     AGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCA          >  SRR4302533.5320361/1‑94 (MQ=60)

                                                                                     AGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCA          >  SRR4302533.6148648/1‑94 (MQ=60)

                                                                                     AGGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCA          >  SRR4302533.3372155/1‑94 (MQ=60)

                                                                                      GGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAG         <  SRR4302533.545880/94‑1 (MQ=60)

                                                                                      GGGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAG         <  SRR4302533.5130877/94‑1 (MQ=60)

                                                                                       GGGTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGA        <  SRR4302533.676603/94‑1 (MQ=60)

                                                                                         GTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATC      >  SRR4302533.408453/1‑94 (MQ=60)

                                                                                         GTTTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATC      <  SRR4302533.4125641/94‑1 (MQ=60)

                                                                                           TTGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTT    <  SRR4302533.4146910/94‑1 (MQ=60)

                                                                                            TGCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTA   >  SRR4302533.5708391/1‑94 (MQ=60)

                                                                                             GCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTAT  >  SRR4302533.3031925/1‑94 (MQ=60)

                                                                                             GCCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTAT  <  SRR4302533.5468265/94‑1 (MQ=60)

ATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTAT  >  NC_000913/747859‑748045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`