Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 905,051 T→C E321G (GAG→GGG)  b0868 ← putative nucleotide di‑P‑sugar epimerase or dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913905,0510TC99.5% 611.9 / NA 190E321G (GAG→GGG) b0868putative nucleotide di‑P‑sugar epimerase or dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (87/102);  minor base G (1/0);  total (88/102)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

GATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTG  >  NC_000913/904961‑905140
                                                                                          |                                                                                         
   tAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTcc                                                                                      <  2:1786189/93‑1 (MQ=255)
   tAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTcc                                                                                      <  1:3774804/93‑1 (MQ=255)
    aGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACTGGCTGATAACCTAACGCTTCCt                                                                                     >  2:149316/1‑93 (MQ=255)
    aGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCt                                                                                     <  2:3013790/93‑1 (MQ=255)
     gcgcGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTg                                                                                    <  1:3263300/93‑1 (MQ=255)
      cgcgGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTgc                                                                                   >  2:5566246/1‑93 (MQ=255)
       gcgGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTgcg                                                                                  >  2:4269753/1‑93 (MQ=255)
       gcgGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTgcg                                                                                  >  1:5221117/1‑93 (MQ=255)
         ggCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCa                                                                                <  1:5634311/93‑1 (MQ=255)
         ggCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCa                                                                                >  1:2608067/1‑93 (MQ=255)
            aGTTTTCCGTGGTCGCGTAGCCAGGTGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCAcgc                                                                             >  1:3116765/1‑93 (MQ=255)
             gTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCAcgcg                                                                            <  2:955641/93‑1 (MQ=255)
              ttttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGt                                                                           >  2:2156890/1‑93 (MQ=255)
               tttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTa                                                                          >  1:1129206/1‑93 (MQ=255)
                ttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTaa                                                                         <  1:2885292/93‑1 (MQ=255)
                ttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTaa                                                                         <  2:2013652/93‑1 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       >  2:6302455/1‑93 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       >  1:1686172/1‑93 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       <  1:166291/93‑1 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       <  2:5884359/93‑1 (MQ=255)
                    gTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATc                                                                     <  2:615480/93‑1 (MQ=255)
                      ggTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCa                                                                   >  1:5467582/1‑93 (MQ=255)
                       gTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAg                                                                  <  1:4393608/93‑1 (MQ=255)
                       gTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAg                                                                  <  2:5666517/93‑1 (MQ=255)
                            gTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTaa                                                             >  2:4373521/1‑93 (MQ=255)
                             tAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTaaa                                                            >  2:5182863/1‑93 (MQ=255)
                             tAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTaaa                                                            <  2:6123966/93‑1 (MQ=255)
                              aGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAg                                                           <  2:3821858/93‑1 (MQ=255)
                                  aGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                                                       >  1:814640/1‑93 (MQ=255)
                                  aGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                                                       >  2:5750783/1‑93 (MQ=255)
                                   ggcggcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaaa                                                      >  1:5986014/1‑93 (MQ=255)
                                    gcggcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaaaa                                                     <  1:5177225/93‑1 (MQ=255)
                                      ggcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAtt                                                   <  1:4937396/93‑1 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  1:5811381/1‑93 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  2:2754041/1‑93 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  <  2:4995172/93‑1 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  1:4261848/1‑93 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  1:4151750/1‑93 (MQ=255)
                                         aGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCTAGCGTAAAGTCAAAATTCaa                                                >  2:3361717/1‑93 (MQ=255)
                                          gTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc                                               >  2:5267840/1‑93 (MQ=255)
                                          gTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc                                               <  2:488209/93‑1 (MQ=255)
                                          gTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc                                               <  1:3467281/93‑1 (MQ=255)
                                           ttttCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACt                                              >  1:1806682/1‑93 (MQ=255)
                                             ttCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTg                                            <  1:1753555/93‑1 (MQ=255)
                                             ttCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCATTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTg                                            >  2:4117522/1‑93 (MQ=255)
                                              tctcGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTgg                                           <  2:5456099/93‑1 (MQ=255)
                                               ctcGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGga                                          <  1:3508939/93‑1 (MQ=255)
                                                tcGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGgag                                         >  1:3688416/1‑93 (MQ=255)
                                                 cGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGgaga                                        <  2:4779371/93‑1 (MQ=255)
                                                  gATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGAc                                       <  2:2840838/93‑1 (MQ=255)
                                                   aTACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACg                                      >  1:4152526/1‑93 (MQ=255)
                                                   aTACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACg                                      >  2:3337876/1‑93 (MQ=255)
                                                   aTACCTTCAACCAGGGTGATGACCGGCTGGTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACg                                      >  2:3168275/1‑93 (MQ=255)
                                                    tACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGc                                     <  1:2962826/93‑1 (MQ=255)
                                                    tACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGc                                     >  1:2834862/1‑93 (MQ=255)
                                                      ccTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCg                                   <  1:4282552/93‑1 (MQ=255)
                                                       cTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGt                                  <  2:3216712/93‑1 (MQ=255)
                                                       cTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGt                                  >  2:2512248/1‑93 (MQ=255)
                                                       cTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGt                                  >  2:4735523/1‑93 (MQ=255)
                                                        ttCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTa                                 >  2:3260888/1‑93 (MQ=255)
                                                        ttCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTa                                 >  1:1043919/1‑93 (MQ=255)
                                                         tCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAg                                <  2:268970/93‑1 (MQ=255)
                                                          cATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGt                               >  2:4563087/1‑93 (MQ=255)
                                                           aTCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTg                              >  1:1562487/1‑93 (MQ=255)
                                                           aTCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTg                              >  1:4542453/1‑93 (MQ=255)
                                                            tCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTgg                             >  2:819309/1‑93 (MQ=255)
                                                              cAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGt                           >  2:876290/1‑93 (MQ=255)
                                                              cAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGt                           >  1:163224/1‑93 (MQ=255)
                                                              cAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGt                           <  2:1043169/93‑1 (MQ=255)
                                                               aGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTc                          <  2:1116770/93‑1 (MQ=255)
                                                                gggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCa                         >  1:2237276/1‑93 (MQ=255)
                                                                gggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCa                         >  2:1506061/1‑93 (MQ=255)
                                                                gggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCa                         >  2:3845944/1‑93 (MQ=255)
                                                                 ggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAg                        >  2:2603686/1‑93 (MQ=255)
                                                                  gTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgc                       <  1:3036685/93‑1 (MQ=255)
                                                                   tgatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcg                      <  1:1919448/93‑1 (MQ=255)
                                                                    gatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcgg                     <  1:2154762/93‑1 (MQ=255)
                                                                    gatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcgg                     >  1:4040370/1‑93 (MQ=255)
                                                                    gatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcgg                     >  2:5244910/1‑93 (MQ=255)
                                                                      tgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                   <  1:2864468/93‑1 (MQ=255)
                                                                      tgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                   >  2:212901/1‑93 (MQ=255)
                                                                      tgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                   >  2:4206164/1‑93 (MQ=255)
                                                                       gaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGcggcgg                  >  1:3915018/1‑93 (MQ=255)
                                                                        aCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCggcggc                 <  2:1743801/93‑1 (MQ=255)
                                                                         ccGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGct                <  1:6035296/93‑1 (MQ=255)
                                                                          cGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTTGGTCAGCGGCGGctc               >  1:5112914/1‑93 (MQ=255)
                                                                          cGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGctc               >  1:2032529/1‑93 (MQ=255)
                                                                             cTGATAACCTATCCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCttt            <  2:3563422/93‑1 (MQ=255)
                                                                             cTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCttt            <  1:913017/93‑1 (MQ=255)
                                                                              tGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCtttt           <  2:3970153/93‑1 (MQ=255)
                                                                               gATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTg          >  1:5399899/1‑93 (MQ=255)
                                                                                aTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGc         <  2:2553396/93‑1 (MQ=255)
                                                                                aTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGc         <  1:1397549/93‑1 (MQ=255)
                                                                                aTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGc         <  2:5958639/93‑1 (MQ=255)
                                                                                  aaCCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTg       >  1:4825175/1‑93 (MQ=255)
                                                                                   aCCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGa      >  2:3791297/1‑93 (MQ=255)
                                                                                     cTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACt    >  2:2473866/1‑93 (MQ=255)
                                                                                      tAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACtt   >  1:3979605/1‑93 (MQ=255)
                                                                                       aaCCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACttc  >  1:656054/1‑92 (MQ=255)
                                                                                       aaCCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTg  >  2:5304264/1‑93 (MQ=255)
                                                                                          |                                                                                         
GATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTG  >  NC_000913/904961‑905140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGC  >  NC_000913/904958‑905144
                                                                                             |                                                                                             
CAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACC                                                                                               >  SRR4302533.3224764/1‑94 (MQ=60)
CAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACC                                                                                               >  SRR4302533.2171578/1‑94 (MQ=60)
CAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACC                                                                                               >  SRR4302533.5786460/1‑94 (MQ=60)
CAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACC                                                                                               >  SRR4302533.1692346/1‑94 (MQ=60)
 AGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCTGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCC                                                                                              >  SRR4302533.4461927/1‑94 (MQ=60)
 AGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCC                                                                                              >  SRR4302533.289998/1‑94 (MQ=60)
 AGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGATTTCTCGATACCTTCATCCAGAGTGATGACCGGCTGATAACCTAACGC                                                                                              >  SRR4302533.1000986/1‑94 (MQ=60)
  GGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCT                                                                                             >  SRR4302533.5385348/1‑94 (MQ=60)
    ATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTC                                                                                           >  SRR4302533.3577520/1‑94 (MQ=60)
      TAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCT                                                                                         <  SRR4302533.1676725/94‑1 (MQ=60)
      TAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCT                                                                                         <  SRR4302533.3797943/94‑1 (MQ=60)
        GCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGC                                                                                       <  SRR4302533.3283430/94‑1 (MQ=60)
         CGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCG                                                                                      <  SRR4302533.289612/94‑1 (MQ=60)
         CGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCG                                                                                      >  SRR4302533.5252536/1‑94 (MQ=60)
         CGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTGCATCCAGGGTGATGACCGGCAGATAACCTAACCCTTCCTGCG                                                                                      <  SRR4302533.1707030/94‑1 (MQ=60)
          GCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGC                                                                                     <  SRR4302533.349594/94‑1 (MQ=60)
           CGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCA                                                                                    >  SRR4302533.2624084/1‑94 (MQ=60)
            GGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCAC                                                                                   <  SRR4302533.3437793/94‑1 (MQ=60)
            GGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCAC                                                                                   <  SRR4302533.5668381/94‑1 (MQ=60)
              CAGTTTTCCGTGGTCGCGTAGCCAGGTGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGC                                                                                 >  SRR4302533.3135926/1‑94 (MQ=60)
                 TTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTA                                                                              >  SRR4302533.1135917/1‑94 (MQ=60)
                   TTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAAT                                                                            <  SRR4302533.792365/94‑1 (MQ=60)
                   TTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAAT                                                                            <  SRR4302533.2903033/94‑1 (MQ=60)
                   TTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAAT                                                                            >  SRR4302533.2838389/1‑94 (MQ=60)
                    TCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATA                                                                           >  SRR4302533.1696386/1‑94 (MQ=60)
                     CCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATAT                                                                          <  SRR4302533.167345/94‑1 (MQ=60)
                      cggggtaggGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGATCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATC                                                                         <  SRR4302533.1809335/85‑1 (MQ=60)
                        TGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTACCCCTTCCTGCGCACGCGTAATATCCA                                                                       <  SRR4302533.4326206/94‑1 (MQ=60)
                        TGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCA                                                                       >  SRR4302533.2931819/1‑94 (MQ=60)
                        TGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCA                                                                       >  SRR4302533.5500535/1‑94 (MQ=60)
                        TGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCA                                                                       >  SRR4302533.1821720/1‑94 (MQ=60)
                          GTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGC                                                                     <  SRR4302533.3752390/94‑1 (MQ=60)
                          GTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGC                                                                     <  SRR4302533.4420133/94‑1 (MQ=60)
                            CGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGT                                                                   <  SRR4302533.2227164/94‑1 (MQ=60)
                               GTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAA                                                                >  SRR4302533.3041924/1‑94 (MQ=60)
                                TAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAG                                                               <  SRR4302533.415671/94‑1 (MQ=60)
                                 AGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGC                                                              >  SRR4302533.4302371/1‑94 (MQ=60)
                                    CAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAA                                                           >  SRR4302533.819409/1‑94 (MQ=60)
                                     AGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAA                                                          <  SRR4302533.6067713/94‑1 (MQ=60)
                                     AGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAA                                                          >  SRR4302533.6022265/1‑94 (MQ=60)
                                       GCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAT                                                        <  SRR4302533.1644666/94‑1 (MQ=60)
                                       GCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAT                                                        >  SRR4302533.3932862/1‑94 (MQ=60)
                                       GCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAT                                                        <  SRR4302533.1612568/94‑1 (MQ=60)
                                       GCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAT                                                        <  SRR4302533.5208493/94‑1 (MQ=60)
                                        CGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATT                                                       >  SRR4302533.1991312/1‑94 (MQ=60)
                                        CGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATT                                                       >  SRR4302533.85384/1‑94 (MQ=60)
                                        CGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATT                                                       <  SRR4302533.4967101/94‑1 (MQ=60)
                                         GGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTC                                                      >  SRR4302533.4176906/1‑94 (MQ=60)
                                         GGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTC                                                      >  SRR4302533.5846477/1‑94 (MQ=60)
                                         GGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTC                                                      <  SRR4302533.4967288/94‑1 (MQ=60)
                                         GGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTC                                                      >  SRR4302533.4287527/1‑94 (MQ=60)
                                          GCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCA                                                     <  SRR4302533.2149112/94‑1 (MQ=60)
                                             GTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACT                                                  >  SRR4302533.1817678/1‑94 (MQ=60)
                                             GTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACT                                                  <  SRR4302533.3488681/94‑1 (MQ=60)
                                              TTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTT                                                 <  SRR4302533.1547914/94‑1 (MQ=60)
                                              TTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTT                                                 <  SRR4302533.1360328/94‑1 (MQ=60)
                                                TTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGG                                               <  SRR4302533.1764238/94‑1 (MQ=60)
                                                TTCTCGATACCTTCATCCAGAGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGG                                               >  SRR4302533.4643033/1‑94 (MQ=60)
                                                  CTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAG                                             <  SRR4302533.514352/94‑1 (MQ=60)
                                                  CTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAG                                             <  SRR4302533.3530574/94‑1 (MQ=60)
                                                  CTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAG                                             >  SRR4302533.3711082/1‑94 (MQ=60)
                                                  CTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCAAGCGTAAAGTCAAAATTCAACTTGGAA                                             >  SRR4302533.1816822/1‑94 (MQ=60)
                                                    CGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGAC                                           <  SRR4302533.5581043/94‑1 (MQ=60)
                                                     GATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACG                                          <  SRR4302533.3283173/94‑1 (MQ=60)
                                                     GATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACG                                          >  SRR4302533.4177686/1‑94 (MQ=60)
                                                     GATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACG                                          <  SRR4302533.2323142/94‑1 (MQ=60)
                                                      ATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGC                                         >  SRR4302533.2852310/1‑94 (MQ=60)
                                                       TACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCC                                        <  SRR4302533.2980939/94‑1 (MQ=60)
                                                        ACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCG                                       >  SRR4302533.3989216/1‑94 (MQ=60)
                                                         CCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGT                                      <  SRR4302533.4308290/94‑1 (MQ=60)
                                                          CTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTA                                     >  SRR4302533.1050055/1‑94 (MQ=60)
                                                           TTCATCCAGAGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAG                                    <  SRR4302533.5825863/94‑1 (MQ=60)
                                                             CATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTG                                  >  SRR4302533.1571892/1‑94 (MQ=60)
                                                             CATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTG                                  >  SRR4302533.4569960/1‑94 (MQ=60)
                                                               TCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGG                                <  SRR4302533.5483975/94‑1 (MQ=60)
                                                                CCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGT                               >  SRR4302533.164264/1‑94 (MQ=60)
                                                                 CAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTACTGGGTC                              >  SRR4302533.3086911/1‑94 (MQ=60)
                                                                  AGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCA                             <  SRR4302533.4061747/94‑1 (MQ=60)
                                                                  AGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCA                             >  SRR4302533.2251105/1‑94 (MQ=60)
                                                                     GTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCG                          <  SRR4302533.3055265/94‑1 (MQ=60)
                                                                      TGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGG                         >  SRR4302533.4064961/1‑94 (MQ=60)
                                                                      TGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGG                         <  SRR4302533.1931165/94‑1 (MQ=60)
                                                                       GATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGC                        <  SRR4302533.2168030/94‑1 (MQ=60)
                                                                         TGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGG                      <  SRR4302533.2882087/94‑1 (MQ=60)
                                                                         TGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGG                      >  SRR4302533.3938933/1‑94 (MQ=60)
                                                                           ACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCT                    <  SRR4302533.5049348/94‑1 (MQ=60)
                                                                            CCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTTGGTCAGCGGCGGCTC                   >  SRR4302533.5143811/1‑94 (MQ=60)
                                                                            CCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTC                   <  SRR4302533.6071871/94‑1 (MQ=60)
                                                                            CCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTC                   >  SRR4302533.2045069/1‑94 (MQ=60)
                                                                             CGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCT                  <  SRR4302533.6276455/94‑1 (MQ=60)
                                                                                CTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTT               <  SRR4302533.918379/94‑1 (MQ=60)
                                                                                 TGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTG              >  SRR4302533.5432346/1‑94 (MQ=60)
                                                                                 TGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTG              <  SRR4302533.5217356/94‑1 (MQ=60)
                                                                                   ATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCT            <  SRR4302533.1405971/94‑1 (MQ=60)
                                                                                    TAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTG           >  SRR4302533.4854404/1‑94 (MQ=60)
                                                                                        CTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTT       >  SRR4302533.4003830/1‑94 (MQ=60)
                                                                                         TAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTG      <  SRR4302533.424686/94‑1 (MQ=60)
                                                                                         TAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTC      >  SRR4302533.659925/1‑94 (MQ=60)
                                                                                          AACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGC     >  SRR4302533.391667/1‑94 (MQ=60)
                                                                                          AACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGC     <  SRR4302533.4537367/94‑1 (MQ=60)
                                                                                           ACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCG    >  SRR4302533.515897/1‑94 (MQ=60)
                                                                                            CCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGG   <  SRR4302533.2005381/94‑1 (MQ=60)
                                                                                            CCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGG   >  SRR4302533.1849530/1‑94 (MQ=60)
                                                                                             CCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGC  >  SRR4302533.2716040/1‑94 (MQ=60)
                                                                                             CCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGC  >  SRR4302533.5864329/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGC  >  NC_000913/904958‑905144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑