Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 963,286 A→G H79R (CAC→CGC)  himD → integration host factor beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913963,2860AG99.6% 755.2 / NA 232H79R (CAC→CGC) himDintegration host factor beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (112/119);  minor base T (0/1);  total (112/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCG  >  NC_000913/963196‑963375
                                                                                          |                                                                                         
gCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCt                                                                                         >  2:4132981/1‑93 (MQ=255)
  aGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCttt                                                                                       >  2:1790052/1‑93 (MQ=255)
  aGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCttt                                                                                       <  2:428918/93‑1 (MQ=255)
  aGTGTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCttt                                                                                       <  2:376382/93‑1 (MQ=255)
   gTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTa                                                                                      >  2:3839431/1‑93 (MQ=255)
   gTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTa                                                                                      >  2:4172886/1‑93 (MQ=255)
    tttCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTaa                                                                                     >  2:681702/1‑93 (MQ=255)
     ttCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTaaa                                                                                    >  1:2994734/1‑93 (MQ=255)
      tctcTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAAc                                                                                   <  2:2814168/93‑1 (MQ=255)
      tctcTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAAc                                                                                   <  1:5746552/93‑1 (MQ=255)
      tctcTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAAc                                                                                   >  2:4601873/1‑93 (MQ=255)
       ctctTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAAcc                                                                                  <  2:2378240/93‑1 (MQ=255)
       ctctTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAAcc                                                                                  <  2:5743448/93‑1 (MQ=255)
        tctTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCt                                                                                 <  1:2404244/93‑1 (MQ=255)
        tctTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCt                                                                                 >  2:2158920/1‑93 (MQ=255)
        tctTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCt                                                                                 >  2:3584880/1‑93 (MQ=255)
         ctTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTg                                                                                <  1:2481852/93‑1 (MQ=255)
          tttGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGACAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTgg                                                                               >  2:4157967/1‑93 (MQ=255)
              cACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTaaa                                                                           <  2:4223203/93‑1 (MQ=255)
               aCTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAg                                                                          >  1:2913674/1‑93 (MQ=255)
                 tACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGaa                                                                        <  2:4489456/93‑1 (MQ=255)
                 tACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGaa                                                                        >  2:4077507/1‑93 (MQ=255)
                 tACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGaa                                                                        <  1:4971692/93‑1 (MQ=255)
                  aCCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAAc                                                                       <  1:4885796/93‑1 (MQ=255)
                   ccGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACt                                                                      <  1:509828/93‑1 (MQ=255)
                         accacGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGa                                                                >  1:2882849/1‑93 (MQ=255)
                          ccacGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGAt                                                               <  1:3505625/93‑1 (MQ=255)
                          ccacGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGAt                                                               <  1:991999/93‑1 (MQ=255)
                          ccacGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGAt                                                               <  1:1810762/93‑1 (MQ=255)
                           cacGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATc                                                              <  2:5693191/93‑1 (MQ=255)
                            acGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATcg                                                             >  1:3639581/1‑93 (MQ=255)
                               tACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCgcgc                                                          <  2:614651/93‑1 (MQ=255)
                                  cGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCaa                                                       <  2:1184280/93‑1 (MQ=255)
                                  cGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCaa                                                       >  1:4148534/1‑93 (MQ=255)
                                  cGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCaa                                                       >  1:6132909/1‑93 (MQ=255)
                                    gACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAata                                                     >  1:480002/1‑93 (MQ=255)
                                    gACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAata                                                     >  1:4557606/1‑93 (MQ=255)
                                    gACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAata                                                     >  1:3943183/1‑93 (MQ=255)
                                    gACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAata                                                     >  2:195689/1‑93 (MQ=255)
                                     aCGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAatat                                                    <  2:2334470/93‑1 (MQ=255)
                                      cGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATAtt                                                   <  1:2254946/93‑1 (MQ=255)
                                       gTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATAttt                                                  >  1:621458/1‑93 (MQ=255)
                                       gTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATAttt                                                  <  2:3894944/93‑1 (MQ=255)
                                         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTAc                                                <  2:1519711/93‑1 (MQ=255)
                                         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTAc                                                >  2:6238891/1‑93 (MQ=255)
                                         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTAc                                                <  1:1192026/93‑1 (MQ=255)
                                         aaTCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATAAGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTAc                                                >  2:910711/1‑93 (MQ=255)
                                           tCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACgg                                              <  2:1345929/93‑1 (MQ=255)
                                           tCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACgg                                              <  1:1721854/93‑1 (MQ=255)
                                             cGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGtt                                            >  2:1097812/1‑93 (MQ=255)
                                             cGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGtt                                            >  1:4352777/1‑93 (MQ=255)
                                              gAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTa                                           >  1:1243517/1‑93 (MQ=255)
                                                aGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAg                                         <  2:4675364/93‑1 (MQ=255)
                                                 gACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGt                                        >  1:103249/1‑93 (MQ=255)
                                                 gACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGt                                        <  2:316553/93‑1 (MQ=255)
                                                  aCTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGtt                                       >  1:3329176/1‑93 (MQ=255)
                                                   cTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGttt                                      >  1:16576/1‑93 (MQ=255)
                                                     ggCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGttttt                                    <  1:4216556/93‑1 (MQ=255)
                                                         aTAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACt                                <  1:804807/93‑1 (MQ=255)
                                                         aTAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACt                                >  1:231876/1‑93 (MQ=255)
                                                          tAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTc                               <  1:67223/93‑1 (MQ=255)
                                                          tAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTc                               >  2:4104732/1‑93 (MQ=255)
                                                          tAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTc                               <  2:5013070/93‑1 (MQ=255)
                                                           aaaGTAGAACTGGAAGAAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCa                              <  2:3133793/93‑1 (MQ=255)
                                                             aGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCaaa                            <  1:5682735/93‑1 (MQ=255)
                                                              gTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAAc                           >  1:2782633/1‑93 (MQ=255)
                                                              gTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAAc                           >  2:5432509/1‑93 (MQ=255)
                                                               tAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACt                          <  2:2449561/93‑1 (MQ=255)
                                                                aGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACtt                         <  2:2993297/93‑1 (MQ=255)
                                                                   aCTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGaa                      <  2:1459061/93‑1 (MQ=255)
                                                                     tGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACg                    >  2:1649288/1‑93 (MQ=255)
                                                                     tGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACg                    >  2:3412539/1‑93 (MQ=255)
                                                                       gaaggaaAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACgag                  >  1:5772749/1‑93 (MQ=255)
                                                                        aaggaaAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACgaga                 <  1:4480572/93‑1 (MQ=255)
                                                                        aagaaaAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACgaga                 <  2:6026710/93‑1 (MQ=255)
                                                                         aggaaAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACgagag                >  1:3380974/1‑93 (MQ=255)
                                                                         aggaaAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACgagag                >  1:5289140/1‑93 (MQ=255)
                                                                            aaaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGaaa             <  1:4744643/93‑1 (MQ=255)
                                                                            aaaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGaaa             <  1:1358308/93‑1 (MQ=255)
                                                                            aaaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGaaa             <  2:4236968/93‑1 (MQ=255)
                                                                             aaaTGCGTTCTTCGCTTTAAACCTGTTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAGGTTTTTTACGCAAACTTGAACGAGAGaaaa            <  2:1731467/93‑1 (MQ=255)
                                                                             aaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAGCGAGAGaaaa            <  2:3249882/93‑1 (MQ=255)
                                                                             aaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGaaaa            <  1:2554245/93‑1 (MQ=255)
                                                                             aaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGaaaa            <  1:5597985/93‑1 (MQ=255)
                                                                             aaaTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGaaaa            >  1:5479205/1‑93 (MQ=255)
                                                                               aTACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGc          >  1:1433189/1‑93 (MQ=255)
                                                                                tACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCa         >  2:5141718/1‑93 (MQ=255)
                                                                                 aCGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAc        >  1:3030804/1‑93 (MQ=255)
                                                                                 aCGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAc        <  2:1426003/93‑1 (MQ=255)
                                                                                  cGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAcc       <  1:374580/93‑1 (MQ=255)
                                                                                   gTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCt      <  1:4648294/93‑1 (MQ=255)
                                                                                   gTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCt      <  2:586024/93‑1 (MQ=255)
                                                                                   gTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCt      <  2:5498305/93‑1 (MQ=255)
                                                                                   gTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCt      <  1:1933565/93‑1 (MQ=255)
                                                                                    ttCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTg     >  2:3424568/1‑93 (MQ=255)
                                                                                    ttCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTg     >  2:4903823/1‑93 (MQ=255)
                                                                                    ttCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTg     <  1:531960/93‑1 (MQ=255)
                                                                                     tCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGt    >  2:5576317/1‑93 (MQ=255)
                                                                                     tCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGt    <  1:3844268/93‑1 (MQ=255)
                                                                                      ccTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTc   <  1:2677365/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCG  >  NC_000913/963196‑963375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTG  >  NC_000913/963194‑963379
                                                                                            |                                                                                             
CGGCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGC                                                                                              >  SRR4302533.4726680/1‑94 (MQ=60)
CGGCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGC                                                                                              >  SRR4302533.2514797/1‑94 (MQ=60)
 GGCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCT                                                                                             >  SRR4302533.981348/1‑94 (MQ=60)
    AGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTA                                                                                          >  SRR4302533.4669440/1‑94 (MQ=60)
     GTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAA                                                                                         >  SRR4302533.2135602/1‑94 (MQ=60)
     GTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAA                                                                                         >  SRR4302533.1710124/1‑94 (MQ=60)
      TTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAA                                                                                        >  SRR4302533.3013046/1‑94 (MQ=60)
        TCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACC                                                                                      <  SRR4302533.5781225/94‑1 (MQ=60)
          TCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTG                                                                                    <  SRR4302533.2419091/94‑1 (MQ=60)
           CTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGG                                                                                   <  SRR4302533.5463771/94‑1 (MQ=60)
           CTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGG                                                                                   <  SRR4302533.5541521/94‑1 (MQ=60)
           CTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGG                                                                                   <  SRR4302533.2497157/94‑1 (MQ=60)
           CTTTGCACTACCGCGCACCACGTACCGGACGTAAGCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGG                                                                                   >  SRR4302533.6181660/1‑94 (MQ=60)
            TTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGT                                                                                  <  SRR4302533.4491720/94‑1 (MQ=60)
               GCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAA                                                                               >  SRR4302533.3242526/1‑94 (MQ=60)
                CACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAG                                                                              >  SRR4302533.2931551/1‑94 (MQ=60)
                CACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAG                                                                              >  SRR4302533.3576698/1‑94 (MQ=60)
                  CTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAA                                                                            >  SRR4302533.4204290/1‑94 (MQ=60)
                  CTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAA                                                                            <  SRR4302533.2429490/94‑1 (MQ=60)
                   TACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAAC                                                                           <  SRR4302533.5001836/94‑1 (MQ=60)
                    ACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACT                                                                          <  SRR4302533.3045441/94‑1 (MQ=60)
                    ACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACT                                                                          <  SRR4302533.4915395/94‑1 (MQ=60)
                    ACCGCGCACCACGAACCGGAGGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACT                                                                          <  SRR4302533.4274721/94‑1 (MQ=60)
                     CCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTG                                                                         <  SRR4302533.512857/94‑1 (MQ=60)
                     CCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTG                                                                         <  SRR4302533.2643803/94‑1 (MQ=60)
                       GCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCG                                                                       <  SRR4302533.1706243/94‑1 (MQ=60)
                          CACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGA                                                                    >  SRR4302533.2900583/1‑94 (MQ=60)
                           ACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGAT                                                                   >  SRR4302533.4776260/1‑94 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGGACTGCGCGATC                                                                  <  SRR4302533.1379717/94‑1 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATC                                                                  <  SRR4302533.997797/94‑1 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATC                                                                  >  SRR4302533.4359217/1‑94 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATC                                                                  <  SRR4302533.5245192/94‑1 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATC                                                                  <  SRR4302533.3527234/94‑1 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATC                                                                  <  SRR4302533.5802891/94‑1 (MQ=60)
                            CCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATC                                                                  <  SRR4302533.1821825/94‑1 (MQ=60)
                             CACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCG                                                                 >  SRR4302533.3661958/1‑94 (MQ=60)
                               CGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCG                                                               <  SRR4302533.2932784/94‑1 (MQ=60)
                                  ACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCA                                                            >  SRR4302533.2709307/1‑94 (MQ=60)
                                  ACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCA                                                            >  SRR4302533.732575/1‑94 (MQ=60)
                                   CCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAA                                                           >  SRR4302533.4173670/1‑94 (MQ=60)
                                   CCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAA                                                           >  SRR4302533.6169859/1‑94 (MQ=60)
                                    CGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAAT                                                          <  SRR4302533.5920479/94‑1 (MQ=60)
                                    CGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAAT                                                          <  SRR4302533.2115413/94‑1 (MQ=60)
                                     GGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATA                                                         >  SRR4302533.3967239/1‑94 (MQ=60)
                                     GGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATA                                                         >  SRR4302533.482869/1‑94 (MQ=60)
                                     GGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATA                                                         >  SRR4302533.4585244/1‑94 (MQ=60)
                                      GACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATAT                                                        <  SRR4302533.2284991/94‑1 (MQ=60)
                                        CGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTT                                                      >  SRR4302533.625108/1‑94 (MQ=60)
                                        CGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTT                                                      <  SRR4302533.5170672/94‑1 (MQ=60)
                                        CGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTT                                                      <  SRR4302533.2268893/94‑1 (MQ=60)
                                         GTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTA                                                     >  SRR4302533.375835/1‑94 (MQ=60)
                                          TAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTAC                                                    >  SRR4302533.91928/1‑94 (MQ=60)
                                           AATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACG                                                   <  SRR4302533.3011060/94‑1 (MQ=60)
                                           AATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACG                                                   <  SRR4302533.1199189/94‑1 (MQ=60)
                                             TCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGT                                                 <  SRR4302533.1732297/94‑1 (MQ=60)
                                              CCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTT                                                >  SRR4302533.4379045/1‑94 (MQ=60)
                                               CGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTA                                               >  SRR4302533.1250944/1‑94 (MQ=60)
                                               CGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTA                                               <  SRR4302533.470636/94‑1 (MQ=60)
                                                  AGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGT                                            >  SRR4302533.2901550/1‑94 (MQ=60)
                                                  AGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGT                                            >  SRR4302533.103896/1‑94 (MQ=60)
                                                  AGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGGTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCACTATTTACGGTTAAGT                                            >  SRR4302533.337968/1‑94 (MQ=60)
                                                   GACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTT                                           >  SRR4302533.3349752/1‑94 (MQ=60)
                                                    ACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTT                                          <  SRR4302533.1220016/94‑1 (MQ=60)
                                                    ACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTT                                          >  SRR4302533.16691/1‑94 (MQ=60)
                                                    ACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTT                                          <  SRR4302533.6293793/94‑1 (MQ=60)
                                                    ACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTCGTAACGAACTGCGCGATCGCGCCAATATTTACAGCTAAGTTT                                          >  SRR4302533.2753079/1‑94 (MQ=60)
                                                       GGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTT                                       <  SRR4302533.4242063/94‑1 (MQ=60)
                                                          GATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACT                                    >  SRR4302533.233309/1‑94 (MQ=60)
                                                          GATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACT                                    >  SRR4302533.3135020/1‑94 (MQ=60)
                                                           ATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTC                                   <  SRR4302533.1812339/94‑1 (MQ=60)
                                                           ATAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTC                                   <  SRR4302533.809535/94‑1 (MQ=60)
                                                            TAAAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCA                                  <  SRR4302533.67660/94‑1 (MQ=60)
                                                              AAGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAA                                >  SRR4302533.3872857/1‑94 (MQ=60)
                                                               AGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAAC                               >  SRR4302533.2799728/1‑94 (MQ=60)
                                                               AGTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAAC                               <  SRR4302533.5717064/94‑1 (MQ=60)
                                                                GTAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACT                              >  SRR4302533.2884058/1‑94 (MQ=60)
                                                                 TAGAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTT                             <  SRR4302533.5344372/94‑1 (MQ=60)
                                                                   GAACTGGAAGGAAAATGCGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGA                           <  SRR4302533.3814396/94‑1 (MQ=60)
                                                                   GAACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGA                           >  SRR4302533.3576423/1‑94 (MQ=60)
                                                                    AACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAA                          >  SRR4302533.1130520/1‑94 (MQ=60)
                                                                    AACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAA                          >  SRR4302533.133568/1‑94 (MQ=60)
                                                                    AACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAA                          <  SRR4302533.2376504/94‑1 (MQ=60)
                                                                    AACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAA                          <  SRR4302533.5101559/94‑1 (MQ=60)
                                                                     ACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAAC                         <  SRR4302533.1311089/94‑1 (MQ=60)
                                                                     ACTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAAC                         <  SRR4302533.248180/94‑1 (MQ=60)
                                                                      CTGGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACG                        >  SRR4302533.3424130/1‑94 (MQ=60)
                                                                        GGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAG                      >  SRR4302533.5807609/1‑94 (MQ=60)
                                                                        GGAAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAG                      <  SRR4302533.4860434/94‑1 (MQ=60)
                                                                          AAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAG                    <  SRR4302533.4507678/94‑1 (MQ=60)
                                                                          AAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAG                    >  SRR4302533.5320818/1‑94 (MQ=60)
                                                                          AAGGAAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAG                    >  SRR4302533.3401844/1‑94 (MQ=60)
                                                                              AAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAA                >  SRR4302533.5512236/1‑94 (MQ=60)
                                                                              AAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAA                <  SRR4302533.1366474/94‑1 (MQ=60)
                                                                              AAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAA                <  SRR4302533.5772946/94‑1 (MQ=60)
                                                                              AAAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAA                <  SRR4302533.4773409/94‑1 (MQ=60)
                                                                               AAATACGTTCCTCTCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAG               <  SRR4302533.5642400/94‑1 (MQ=60)
                                                                               AAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAG               <  SRR4302533.5631808/94‑1 (MQ=60)
                                                                               AAATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAG               <  SRR4302533.2569959/94‑1 (MQ=60)
                                                                                AATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGC              >  SRR4302533.1441847/1‑94 (MQ=60)
                                                                                AATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGC              <  SRR4302533.4896837/94‑1 (MQ=60)
                                                                                 ATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCA             >  SRR4302533.5357747/1‑94 (MQ=60)
                                                                                 ATACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCA             >  SRR4302533.2502499/1‑94 (MQ=60)
                                                                                  TACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAC            >  SRR4302533.4089443/1‑94 (MQ=60)
                                                                                  TACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAC            <  SRR4302533.6225658/94‑1 (MQ=60)
                                                                                  TACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAC            >  SRR4302533.3049350/1‑94 (MQ=60)
                                                                                  TACGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCAC            <  SRR4302533.499503/94‑1 (MQ=60)
                                                                                    CGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCT          <  SRR4302533.376843/94‑1 (MQ=60)
                                                                                    CGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCT          <  SRR4302533.4629997/94‑1 (MQ=60)
                                                                                    CGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCT          <  SRR4302533.1463171/94‑1 (MQ=60)
                                                                                    CGTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCT          <  SRR4302533.5767463/94‑1 (MQ=60)
                                                                                     GTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTG         <  SRR4302533.1945363/94‑1 (MQ=60)
                                                                                     GTTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTG         <  SRR4302533.4676459/94‑1 (MQ=60)
                                                                                      TTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGT        >  SRR4302533.1905166/1‑94 (MQ=60)
                                                                                      TTCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGT        <  SRR4302533.535123/94‑1 (MQ=60)
                                                                                       TCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTC       <  SRR4302533.3867747/94‑1 (MQ=60)
                                                                                       TCCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTC       <  SRR4302533.4563456/94‑1 (MQ=60)
                                                                                        CCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCG      <  SRR4302533.2693779/94‑1 (MQ=60)
                                                                                        CCTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCG      <  SRR4302533.3919252/94‑1 (MQ=60)
                                                                                         CTCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGG     >  SRR4302533.1244225/1‑94 (MQ=60)
                                                                                          TCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGG    <  SRR4302533.1659281/94‑1 (MQ=60)
                                                                                          TCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGG    >  SRR4302533.950649/1‑94 (MQ=60)
                                                                                          TCGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGG    <  SRR4302533.3606958/94‑1 (MQ=60)
                                                                                           CGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGT   <  SRR4302533.5423081/94‑1 (MQ=60)
                                                                                           CGCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGCTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGT   >  SRR4302533.3071371/1‑94 (MQ=60)
                                                                                            GCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTG  <  SRR4302533.6152659/94‑1 (MQ=60)
                                                                                            GCTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTG  <  SRR4302533.3105088/94‑1 (MQ=60)
                                                                                            |                                                                                             
CGGCAGTTTCTCTTTGCACTACCGCGCACCACGTACCGGACGTAATCCGAAGACTGGCGATAAAGTAGAACTGGAAGGAAAATACGTTCCTCACTTTAAACCTGGTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTG  >  NC_000913/963194‑963379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑