Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,719	C→T	T48M (ACG→ATG)	hyaE →	HyaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,719	0	C	T	99.6%	859.0 / NA	231	T48M (ACG→ATG)	hyaE	HyaE
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (110/120); total (110/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

gCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgcatgc                                                                                         >  1:4922609/1‑93 (MQ=255)

   ggACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCag                                                                                      >  1:5475959/1‑93 (MQ=255)

   ggACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCag                                                                                      <  1:2115025/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  1:4043336/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  2:1009662/93‑1 (MQ=255)

    gACGCCAGTCAGTGAACCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  2:2440744/93‑1 (MQ=255)

       gCCAGTCAGTTAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGt                                                                                  <  2:641009/93‑1 (MQ=255)

        ccAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTc                                                                                 <  2:322925/93‑1 (MQ=255)

        ccAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTc                                                                                 <  1:5064182/93‑1 (MQ=255)

         cagtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCa                                                                                >  2:82334/1‑93 (MQ=255)

         cagtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCa                                                                                <  2:534057/93‑1 (MQ=255)

          agtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAg                                                                               >  1:1954220/1‑93 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              <  1:4791391/93‑1 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              <  1:3623677/93‑1 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGCGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              >  1:1514902/1‑93 (MQ=255)

            ccagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGGGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCg                                                                             >  2:4067801/2‑93 (MQ=255)

              agtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGAt                                                                           <  1:919196/93‑1 (MQ=255)

                tGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         >  1:1293627/1‑93 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATc                                                                       <  2:5410243/93‑1 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGGCAGCGATAATc                                                                       >  1:1387011/1‑93 (MQ=255)

                   aTCCCGTCTTGACGACTGTCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      <  2:5546031/93‑1 (MQ=255)

                   aTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      <  2:1309621/93‑1 (MQ=255)

                    tCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     >  2:1369987/1‑93 (MQ=255)

                    acccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     <  1:2021680/92‑1 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCgg                                                                    >  1:1725779/1‑93 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAAGTCAGCGATAATCCgg                                                                    >  2:1983906/1‑93 (MQ=255)

                      ccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGt                                                                   >  2:4536374/1‑93 (MQ=255)

                       cGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTa                                                                  >  2:1590776/1‑93 (MQ=255)

                       cGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTa                                                                  >  2:1795136/1‑93 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 >  1:3651347/1‑93 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 >  1:76349/1‑93 (MQ=255)

                         tCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAt                                                                >  1:1310662/1‑93 (MQ=255)

                         tCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAt                                                                >  1:1089588/1‑93 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAtt                                                            >  1:6203646/1‑93 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  2:5833546/93‑1 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  1:6104048/93‑1 (MQ=255)

                               cgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTgg                                                          <  2:5249200/93‑1 (MQ=255)

                               cgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTgg                                                          <  1:247256/93‑1 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         >  2:2223189/1‑93 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         >  1:3531901/1‑93 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         <  2:390555/93‑1 (MQ=255)

                                 acTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCg                                                        <  1:386133/93‑1 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       <  2:4157038/93‑1 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       <  1:2917672/93‑1 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       >  1:2555558/1‑93 (MQ=255)

                                   tGGCTTCCGCAGGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      <  2:5444349/93‑1 (MQ=255)

                                   tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      >  2:3696364/1‑93 (MQ=255)

                                    ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                                                     >  1:867789/1‑93 (MQ=255)

                                     gCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                                                    >  2:3308092/1‑93 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  1:6042714/93‑1 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  1:2686066/93‑1 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  2:2842871/93‑1 (MQ=255)

                                       ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                                                  >  2:4852919/1‑93 (MQ=255)

                                          cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc                                               <  1:2165357/93‑1 (MQ=255)

                                           gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg                                              >  1:112054/1‑93 (MQ=255)

                                           gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg                                              >  1:1176053/1‑93 (MQ=255)

                                              aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGa                                           <  2:3450283/93‑1 (MQ=255)

                                                 gcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGtt                                        <  2:2623741/93‑1 (MQ=255)

                                                    aGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc                                     <  1:3195839/93‑1 (MQ=255)

                                                    aGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc                                     >  2:2765436/1‑93 (MQ=255)

                                                     gACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTccc                                    <  2:3186509/93‑1 (MQ=255)

                                                       cGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGa                                  <  2:4942065/93‑1 (MQ=255)

                                                        ggCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc                                 >  1:2955906/1‑93 (MQ=255)

                                                        ggCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc                                 >  1:4500115/1‑93 (MQ=255)

                                                          cGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACta                               >  1:1837775/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  2:1508164/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  1:3694753/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  1:4077328/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  2:6215345/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            >  1:5545847/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            >  2:1485333/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            >  2:5885739/1‑93 (MQ=255)

                                                              gtgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACa                           <  1:4807235/93‑1 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          >  1:5805215/1‑93 (MQ=255)

                                                                 ttattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATgg                        <  1:5124961/93‑1 (MQ=255)

                                                                 ttattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATgg                        <  2:4499844/93‑1 (MQ=255)

                                                                  tattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGc                       <  2:256388/93‑1 (MQ=255)

                                                                   attaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCa                      >  1:2845484/1‑93 (MQ=255)

                                                                     taAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAgg                    >  2:5105073/1‑93 (MQ=255)

                                                                     taAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAgg                    >  1:4354671/1‑93 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGt                   <  1:5212068/93‑1 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 >  1:4517342/1‑93 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 >  2:2193826/1‑93 (MQ=255)

                                                                         cAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGc                <  2:1848111/93‑1 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCg               >  1:1776204/1‑93 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGGGGCg               >  2:5627243/1‑93 (MQ=255)

                                                                           gTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGa              >  2:3826663/1‑93 (MQ=255)

                                                                            tGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAt             <  2:4292419/93‑1 (MQ=255)

                                                                               cccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGc          <  1:23700/93‑1 (MQ=255)

                                                                                ccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCt         >  2:5299552/1‑93 (MQ=255)

                                                                                 cGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTg        >  1:5901913/1‑93 (MQ=255)

                                                                                 cGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTg        >  1:3056001/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       >  2:1169342/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       >  2:3897687/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       >  1:4233961/1‑93 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      <  2:5148945/93‑1 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      >  1:715222/1‑93 (MQ=255)

                                                                                    aaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc     >  2:176639/1‑93 (MQ=255)

                                                                                      cgcatgcCAGAGGTCAGCTATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCtt   <  1:2037618/93‑1 (MQ=255)

                                                                                       gcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg  >  2:4721297/1‑93 (MQ=255)

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGC  >  NC_000913/1035628‑1035811

GGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGC                                                                                            <  SRR4302533.105053/94‑1 (MQ=60)

GGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGC                                                                                            >  SRR4302533.4952430/1‑94 (MQ=60)

 GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCC                                                                                           <  SRR4302533.2951621/94‑1 (MQ=60)

  CTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCA                                                                                          <  SRR4302533.722038/94‑1 (MQ=60)

  CTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCA                                                                                          <  SRR4302533.1273577/94‑1 (MQ=60)

   TGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAG                                                                                         >  SRR4302533.5508966/1‑94 (MQ=60)

    GGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGA                                                                                        <  SRR4302533.2128070/94‑1 (MQ=60)

     GACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAG                                                                                       <  SRR4302533.4067947/94‑1 (MQ=60)

     GACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAG                                                                                       <  SRR4302533.4098597/94‑1 (MQ=60)

     GACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAG                                                                                       >  SRR4302533.6311587/1‑94 (MQ=60)

     GACGCCACTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAG                                                                                       <  SRR4302533.1135201/94‑1 (MQ=60)

      ACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGG                                                                                      <  SRR4302533.4009291/94‑1 (MQ=60)

        GCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTC                                                                                    <  SRR4302533.1329348/94‑1 (MQ=60)

        GCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTC                                                                                    >  SRR4302533.1312720/1‑94 (MQ=60)

         CCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCA                                                                                   <  SRR4302533.4633939/94‑1 (MQ=60)

         CCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCA                                                                                   <  SRR4302533.5094775/94‑1 (MQ=60)

          CAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAG                                                                                  >  SRR4302533.1966128/1‑94 (MQ=60)

           AGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGC                                                                                 <  SRR4302533.5610473/94‑1 (MQ=60)

           AGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGC                                                                                 >  SRR4302533.1925194/1‑94 (MQ=60)

           AGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGCGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGC                                                                                 >  SRR4302533.1524026/1‑94 (MQ=60)

            GTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCG                                                                                <  SRR4302533.4820424/94‑1 (MQ=60)

            GTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCG                                                                                <  SRR4302533.3645977/94‑1 (MQ=60)

            GTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCAAGAGGTCAGCG                                                                                >  SRR4302533.4835040/1‑94 (MQ=60)

              CAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGCCCCGAAACGCATGCCAGAGGTCAGCGAT                                                                              <  SRR4302533.1749780/94‑1 (MQ=60)

               AGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATA                                                                             >  SRR4302533.5027958/1‑94 (MQ=60)

               AGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATA                                                                             <  SRR4302533.924595/94‑1 (MQ=60)

                GTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAA                                                                            >  SRR4302533.1301418/1‑94 (MQ=60)

                 TGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAAT                                                                           >  SRR4302533.6045353/1‑94 (MQ=60)

                  GAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGGCAGCGATAATC                                                                          >  SRR4302533.1395359/1‑94 (MQ=60)

                   AATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCC                                                                         >  SRR4302533.6331924/1‑94 (MQ=60)

                   AATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCC                                                                         <  SRR4302533.2792101/94‑1 (MQ=60)

                     TCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGG                                                                       <  SRR4302533.1926385/94‑1 (MQ=60)

                     TCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGG                                                                       <  SRR4302533.2034131/94‑1 (MQ=60)

                     TCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGG                                                                       >  SRR4302533.1736252/1‑94 (MQ=60)

                     ACCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGG                                                                       <  SRR4302533.2034130/94‑1 (MQ=60)

                       CCTTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTA                                                                     <  SRR4302533.4092017/94‑1 (MQ=60)

                       CCGTCTTGACGACTGGCTTACGCAAGCGCCAGCCGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTA                                                                     <  SRR4302533.3096419/94‑1 (MQ=60)

                       CCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTA                                                                     <  SRR4302533.4493882/94‑1 (MQ=60)

                        CGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAA                                                                    >  SRR4302533.3673796/1‑94 (MQ=60)

                        CGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAA                                                                    >  SRR4302533.76841/1‑94 (MQ=60)

                         GTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAT                                                                   >  SRR4302533.1096019/1‑94 (MQ=60)

                         GTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAT                                                                   >  SRR4302533.1318529/1‑94 (MQ=60)

                            TTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAT                                                                <  SRR4302533.4434824/94‑1 (MQ=60)

                            TTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAT                                                                <  SRR4302533.6014363/94‑1 (MQ=60)

                             TGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATT                                                               <  SRR4302533.2058827/94‑1 (MQ=60)

                             TGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATT                                                               >  SRR4302533.6240850/1‑94 (MQ=60)

                              GACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTG                                                              <  SRR4302533.4544670/94‑1 (MQ=60)

                               ACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGG                                                             <  SRR4302533.6140892/94‑1 (MQ=60)

                                CGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGC                                                            <  SRR4302533.2021164/94‑1 (MQ=60)

                                CGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGC                                                            >  SRR4302533.3553668/1‑94 (MQ=60)

                                CGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGC                                                            <  SRR4302533.248784/94‑1 (MQ=60)

                                  ACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGA                                                          >  SRR4302533.2571277/1‑94 (MQ=60)

                                  ACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGA                                                          <  SRR4302533.388463/94‑1 (MQ=60)

                                  ACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGA                                                          <  SRR4302533.3663607/94‑1 (MQ=60)

                                   CTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAA                                                         <  SRR4302533.2935556/94‑1 (MQ=60)

                                   CTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAA                                                         <  SRR4302533.4609628/94‑1 (MQ=60)

                                    TGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAT                                                        >  SRR4302533.6113761/1‑94 (MQ=60)

                                    TGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAT                                                        >  SRR4302533.872874/1‑94 (MQ=60)

                                      GCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTA                                                      <  SRR4302533.5164021/94‑1 (MQ=60)

                                       CTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAC                                                     <  SRR4302533.6079324/94‑1 (MQ=60)

                                       CTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAC                                                     <  SRR4302533.2702532/94‑1 (MQ=60)

                                        TTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACT                                                    <  SRR4302533.1380877/94‑1 (MQ=60)

                                         TACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTG                                                   >  SRR4302533.2997920/1‑94 (MQ=60)

                                           CGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCG                                                 >  SRR4302533.1183110/1‑94 (MQ=60)

                                           CGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCG                                                 <  SRR4302533.2178677/94‑1 (MQ=60)

                                           CGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCG                                                 >  SRR4302533.112760/1‑94 (MQ=60)

                                           CGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCG                                                 <  SRR4302533.4237544/94‑1 (MQ=60)

                                               AGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAG                                             <  SRR4302533.2451342/94‑1 (MQ=60)

                                                 CGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTAGCGAATTACTGCGCGAGTT                                           >  SRR4302533.5639044/1‑94 (MQ=60)

                                                 CCCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTT                                           <  SRR4302533.1126355/94‑1 (MQ=60)

                                                  GCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTT                                          >  SRR4302533.3121717/1‑94 (MQ=60)

                                                    CAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCC                                        >  SRR4302533.1770555/1‑94 (MQ=60)

                                                     AGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCC                                       <  SRR4302533.1214948/94‑1 (MQ=60)

                                                     AGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCC                                       >  SRR4302533.2673506/1‑94 (MQ=60)

                                                     AGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCC                                       <  SRR4302533.3215503/94‑1 (MQ=60)

                                                       ACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGA                                     >  SRR4302533.5222934/1‑94 (MQ=60)

                                                        CGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAC                                    >  SRR4302533.2973978/1‑94 (MQ=60)

                                                        CGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAC                                    <  SRR4302533.6195982/94‑1 (MQ=60)

                                                        CGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAC                                    <  SRR4302533.5297916/94‑1 (MQ=60)

                                                        CGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAC                                    >  SRR4302533.4527370/1‑94 (MQ=60)

                                                          GCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTA                                  >  SRR4302533.1848983/1‑94 (MQ=60)

                                                           CGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTAT                                 <  SRR4302533.15063/94‑1 (MQ=60)

                                                           CGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTAT                                 >  SRR4302533.3717446/1‑94 (MQ=60)

                                                           CGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTAT                                 <  SRR4302533.3564792/94‑1 (MQ=60)

                                                           CGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTAT                                 >  SRR4302533.4102149/1‑94 (MQ=60)

                                                            GTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATA                                >  SRR4302533.3799894/1‑94 (MQ=60)

                                                            GTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATA                                <  SRR4302533.4912734/94‑1 (MQ=60)

                                                             TGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAC                               >  SRR4302533.2221879/1‑94 (MQ=60)

                                                             TGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAC                               >  SRR4302533.5579343/1‑94 (MQ=60)

                                                             TGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAC                               <  SRR4302533.5552511/94‑1 (MQ=60)

                                                             TGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAC                               <  SRR4302533.5914651/94‑1 (MQ=60)

                                                             TGGGGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAC                               >  SRR4302533.1686091/1‑94 (MQ=60)

                                                              GGTGTTATTAAGCAGTGACCCGAAACGCATGACAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACA                              <  SRR4302533.2913283/94‑1 (MQ=60)

                                                               GTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAT                             >  SRR4302533.5840270/1‑94 (MQ=60)

                                                               GTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAT                             <  SRR4302533.3826509/94‑1 (MQ=60)

                                                               GTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAT                             <  SRR4302533.4836342/94‑1 (MQ=60)

                                                                TGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATG                            >  SRR4302533.267579/1‑94 (MQ=60)

                                                                TGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATG                            >  SRR4302533.2055645/1‑94 (MQ=60)

                                                                  TTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGC                          <  SRR4302533.5155920/94‑1 (MQ=60)

                                                                   TATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCA                         >  SRR4302533.2862995/1‑94 (MQ=60)

                                                                    ATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAG                        <  SRR4302533.856150/94‑1 (MQ=60)

                                                                     TTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGG                       >  SRR4302533.4380955/1‑94 (MQ=60)

                                                                       AAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTG                     <  SRR4302533.5243456/94‑1 (MQ=60)

                                                                       AAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTG                     <  SRR4302533.1798851/94‑1 (MQ=60)

                                                                        AGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGG                    >  SRR4302533.4544699/1‑94 (MQ=60)

                                                                         GCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGC                   >  SRR4302533.910967/1‑94 (MQ=60)

                                                                          CAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCG                  >  SRR4302533.3225236/1‑94 (MQ=60)

                                                                          CAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCG                  >  SRR4302533.1787018/1‑94 (MQ=60)

                                                                             TGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATT               <  SRR4302533.2915030/94‑1 (MQ=60)

                                                                              GACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTG              <  SRR4302533.838599/94‑1 (MQ=60)

                                                                               ACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGC             >  SRR4302533.3697299/1‑94 (MQ=60)

                                                                                CCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCT            <  SRR4302533.23858/94‑1 (MQ=60)

                                                                                 CCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTG           <  SRR4302533.4296670/94‑1 (MQ=60)

                                                                                 CCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTG           >  SRR4302533.3074739/1‑94 (MQ=60)

                                                                                 CCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTG           >  SRR4302533.5101725/1‑94 (MQ=60)

                                                                                 CCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTG           >  SRR4302533.3626334/1‑94 (MQ=60)

                                                                                 CCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTG           >  SRR4302533.5937554/1‑94 (MQ=60)

                                                                                  CGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGA          <  SRR4302533.2300953/94‑1 (MQ=60)

                                                                                  CGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGA          >  SRR4302533.4259547/1‑94 (MQ=60)

                                                                                   GAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAC         >  SRR4302533.719441/1‑94 (MQ=60)

                                                                                   GAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAC         <  SRR4302533.4975891/94‑1 (MQ=60)

                                                                                    AAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACC        >  SRR4302533.2178201/1‑94 (MQ=60)

                                                                                       CGCATGCCAGAGGTCAGCTATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG     <  SRR4302533.2050185/94‑1 (MQ=60)

                                                                                       CGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG     <  SRR4302533.2557059/94‑1 (MQ=60)

                                                                                         CATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAG   <  SRR4302533.4486840/94‑1 (MQ=60)

                                                                                         CATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAG   <  SRR4302533.4669985/94‑1 (MQ=60)

                                                                                          ATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGC  >  SRR4302533.4073909/1‑94 (MQ=60)

                                                                                          ATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGC  >  SRR4302533.4608028/1‑94 (MQ=60)

                                                                                          ATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGC  <  SRR4302533.4966171/94‑1 (MQ=60)

GGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGC  >  NC_000913/1035628‑1035811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`