Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,293,679	A→G	P187P (CCT→CCC)	ychG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,293,679	0	A	G	99.6%	721.5 / ‑4.8	224	P187P (CCT→CCC)	ychG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (110/113); total (110/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

Only 100 of 238 total aligned reads displayed.

GGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAG  >  NC_000913/1293589‑1293768

ggTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACggg                                                                                         >  2:2875180/1‑93 (MQ=255)

   ttATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATa                                                                                      <  1:2533736/93‑1 (MQ=255)

   ttATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATa                                                                                      >  2:2038764/1‑93 (MQ=255)

    tATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATaa                                                                                     >  2:3634202/1‑93 (MQ=255)

    tATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATaa                                                                                     >  1:2784445/1‑93 (MQ=255)

    tATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATaa                                                                                     >  2:5507360/1‑93 (MQ=255)

    tATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATaa                                                                                     <  2:4391587/93‑1 (MQ=255)

     aTGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAAc                                                                                    >  2:1014102/1‑93 (MQ=255)

      tGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACg                                                                                   >  1:2106101/1‑93 (MQ=255)

      tGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACg                                                                                   <  1:4735687/93‑1 (MQ=255)

      tGGAAAAGTTGCTTTGAGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACg                                                                                   <  1:1416224/93‑1 (MQ=255)

       ggAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACgg                                                                                  <  1:1218881/93‑1 (MQ=255)

        gAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACggg                                                                                 <  1:4564973/93‑1 (MQ=255)

        gAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACggg                                                                                 <  1:3514442/93‑1 (MQ=255)

        gAAAAGGTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATTACggg                                                                                 >  2:2227067/1‑93 (MQ=255)

           aaGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGttt                                                                              <  2:4914112/93‑1 (MQ=255)

            aGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGtttt                                                                             <  1:688302/93‑1 (MQ=255)

              ttGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTg                                                                           <  2:515349/93‑1 (MQ=255)

                gCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGat                                                                         >  1:3430672/1‑93 (MQ=255)

                catttGGGTAAACAAAAAATGCGGCCCAAGAAGGGCACTGCCGTTCACTTAAGAGGAGCGGCACTATGTGTCACGGGATAACGGGTTTTTGat                                                                         <  2:2489692/91‑1 (MQ=255)

                 cTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGata                                                                        >  1:4879245/1‑93 (MQ=255)

                 cTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGata                                                                        <  1:4523108/93‑1 (MQ=255)

                  tttGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGatat                                                                       >  2:2926050/1‑93 (MQ=255)

                    tGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCt                                                                     <  2:5060477/93‑1 (MQ=255)

                     gggTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCtt                                                                    <  2:1481910/93‑1 (MQ=255)

                     gggTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCtt                                                                    >  2:1428929/1‑93 (MQ=255)

                      ggTAAACACAAAATACGGCCCCAGACGGGCACTGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAGCGGGTTTTTGATATCTTa                                                                   <  2:463345/93‑1 (MQ=255)

                      ggTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTa                                                                   <  2:2620005/93‑1 (MQ=255)

                      ggTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTa                                                                   <  1:6189388/93‑1 (MQ=255)

                        tAAACAAAAAATCCGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTCCACGGGATAACGGGTTTTTGATATCTTAAc                                                                 <  1:1919986/93‑1 (MQ=255)

                        tAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAAGGGGTTTTTGATATCTTagc                                                                 <  1:3574366/93‑3 (MQ=255)

                        tAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAc                                                                 >  2:3232837/1‑93 (MQ=255)

                        tAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAc                                                                 >  1:6294588/1‑93 (MQ=255)

                        tAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAc                                                                 >  1:4746068/1‑93 (MQ=255)

                         aaaCAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAcc                                                                >  2:5140566/1‑93 (MQ=255)

                         aaaCAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAcc                                                                >  1:746874/1‑93 (MQ=255)

                          aaCAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCg                                                               >  1:100902/1‑93 (MQ=255)

                          aaCAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCg                                                               >  2:5973054/1‑93 (MQ=255)

                            cAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGAc                                                             <  1:133035/93‑1 (MQ=255)

                            cAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGAc                                                             <  1:3613764/93‑1 (MQ=255)

                             aaaaaaTACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGAcc                                                            >  2:5676946/1‑93 (MQ=255)

                              aaaaaTACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCt                                                           >  1:1747871/1‑93 (MQ=255)

                               aaaaTACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTc                                                          <  2:2581328/93‑1 (MQ=255)

                                aaaTACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCg                                                         <  1:5124722/93‑1 (MQ=255)

                                   tACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGcc                                                      >  2:3554682/1‑93 (MQ=255)

                                   tACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGcc                                                      >  1:2324108/1‑93 (MQ=255)

                                     cGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCtt                                                    >  1:4381052/1‑93 (MQ=255)

                                       gCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGa                                                  >  1:3860137/1‑93 (MQ=255)

                                       gCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGa                                                  <  2:2479287/93‑1 (MQ=255)

                                         cccAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATg                                                <  2:1221887/93‑1 (MQ=255)

                                         cccAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATg                                                >  2:5961187/1‑93 (MQ=255)

                                          ccAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATgg                                               >  2:5823569/1‑93 (MQ=255)

                                          ccAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATgg                                               <  1:4465917/93‑1 (MQ=255)

                                          ccAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATgg                                               <  2:475732/93‑1 (MQ=255)

                                           cAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGt                                              >  2:5407157/1‑93 (MQ=255)

                                            aGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTc                                             >  1:1782878/1‑93 (MQ=255)

                                            aGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTc                                             >  1:4244628/1‑93 (MQ=255)

                                             gAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCg                                            >  1:3013116/1‑93 (MQ=255)

                                             gAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCg                                            >  1:5870058/1‑93 (MQ=255)

                                              aaGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCgg                                           >  2:4857459/1‑93 (MQ=255)

                                               aGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCggg                                          <  2:4238160/93‑1 (MQ=255)

                                               aGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCggg                                          <  1:1659746/93‑1 (MQ=255)

                                                gggCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCgggg                                         <  2:1933179/93‑1 (MQ=255)

                                                gggCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCgggg                                         <  1:5830847/93‑1 (MQ=255)

                                                 ggCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCggggg                                        <  2:1721125/93‑1 (MQ=255)

                                                         cGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGt                                >  2:4369860/1‑93 (MQ=255)

                                                          gTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGtt                               <  1:4991468/93‑1 (MQ=255)

                                                            tCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTAt                             >  1:2961079/1‑93 (MQ=255)

                                                            tCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTAt                             >  2:3958837/1‑93 (MQ=255)

                                                             cACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATg                            <  1:1594584/93‑1 (MQ=255)

                                                             cACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATg                            >  1:5953499/1‑93 (MQ=255)

                                                               cTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGaa                          <  1:310466/93‑1 (MQ=255)

                                                               cTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGaa                          >  2:3308819/1‑93 (MQ=255)

                                                                 tAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAaca                        >  1:1185858/1‑93 (MQ=255)

                                                                 tAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAaca                        >  1:845602/1‑93 (MQ=255)

                                                                   agagGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAcacc                      <  2:350342/93‑1 (MQ=255)

                                                                    gaggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAcgcca                     >  2:740224/1‑93 (MQ=255)

                                                                    gaggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAcacca                     <  1:2425885/93‑1 (MQ=255)

                                                                    gaggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAcacca                     >  2:692638/1‑93 (MQ=255)

                                                                     aggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAcaccac                    <  1:5717480/93‑1 (MQ=255)

                                                                      ggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACt                   >  2:5493863/1‑93 (MQ=255)

                                                                      ggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACt                   <  2:1368685/93‑1 (MQ=255)

                                                                      ggagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACt                   >  2:4071934/1‑93 (MQ=255)

                                                                       gagCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACtt                  <  2:4703198/93‑1 (MQ=255)

                                                                         gCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTcc                >  2:2795784/1‑93 (MQ=255)

                                                                         gCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTcc                >  2:3014238/1‑93 (MQ=255)

                                                                            gCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCaga             <  1:2287773/93‑1 (MQ=255)

                                                                             cACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCagag            >  2:458652/1‑93 (MQ=255)

                                                                             cACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCagag            >  2:1789603/1‑93 (MQ=255)

                                                                              aCTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGc           >  1:2166860/1‑93 (MQ=255)

                                                                               cTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCa          <  1:3849754/93‑1 (MQ=255)

                                                                                tATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCAc         >  2:6184319/1‑93 (MQ=255)

                                                                                tATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCAc         >  1:3337296/1‑93 (MQ=255)

                                                                                   gTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCAcccc      <  1:5396375/93‑1 (MQ=255)

                                                                                      tCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGaa   <  2:3899050/93‑1 (MQ=255)

                                                                                      tCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGaa   >  1:4020832/1‑93 (MQ=255)

                                                                                       cacgGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAg  >  1:2675707/1‑93 (MQ=255)

                                                                                       cacgGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAg  >  1:1824562/1‑93 (MQ=255)

                                                                                       cacgGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAg  >  2:5015292/1‑93 (MQ=255)

                                                                                       cacgGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAg  <  1:5606859/93‑1 (MQ=255)

GGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAG  >  NC_000913/1293589‑1293768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGC  >  NC_000913/1293586‑1293772

GTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACG                                                                                               <  SRR4302533.664221/94‑1 (MQ=60)

  CGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGG                                                                                             <  SRR4302533.378546/94‑1 (MQ=60)

  CGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGG                                                                                             <  SRR4302533.3630046/94‑1 (MQ=60)

    GTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGAT                                                                                           <  SRR4302533.5138019/94‑1 (MQ=60)

     TTTATGGAAAAGTTGCTTTGGATAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATA                                                                                          <  SRR4302533.2686850/94‑1 (MQ=60)

      TTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAA                                                                                         <  SRR4302533.2549335/94‑1 (MQ=60)

      TTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAA                                                                                         >  SRR4302533.2801553/1‑94 (MQ=60)

        ATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACG                                                                                       >  SRR4302533.2119087/1‑94 (MQ=60)

         TGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGG                                                                                      <  SRR4302533.4764394/94‑1 (MQ=60)

         TGGAAAAGTTGCTTTGAGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGG                                                                                      <  SRR4302533.1424791/94‑1 (MQ=60)

          GGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGG                                                                                     <  SRR4302533.4454425/94‑1 (MQ=60)

          GGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGG                                                                                     <  SRR4302533.1226199/94‑1 (MQ=60)

           GAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGT                                                                                    <  SRR4302533.3536104/94‑1 (MQ=60)

           GAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGT                                                                                    <  SRR4302533.3993165/94‑1 (MQ=60)

           GAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGT                                                                                    <  SRR4302533.4592649/94‑1 (MQ=60)

             AAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTT                                                                                  <  SRR4302533.5767208/94‑1 (MQ=60)

               AGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTT                                                                                <  SRR4302533.692372/94‑1 (MQ=60)

                GTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTG                                                                               <  SRR4302533.1223901/94‑1 (MQ=60)

                 TTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGA                                                                              <  SRR4302533.1790363/94‑1 (MQ=60)

                  TGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGAT                                                                             >  SRR4302533.3451865/1‑94 (MQ=60)

                  TGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGAT                                                                             <  SRR4302533.634530/94‑1 (MQ=60)

                   GCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATA                                                                            >  SRR4302533.4908805/1‑94 (MQ=60)

                    CTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATAT                                                                           <  SRR4302533.4550488/94‑1 (MQ=60)

                    CTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATAT                                                                           >  SRR4302533.182107/1‑94 (MQ=60)

                    CTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATAT                                                                           <  SRR4302533.1880861/94‑1 (MQ=60)

                     TTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATC                                                                          <  SRR4302533.3895416/94‑1 (MQ=60)

                     TTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATC                                                                          <  SRR4302533.1135500/94‑1 (MQ=60)

                     TTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATC                                                                          <  SRR4302533.724866/94‑1 (MQ=60)

                      TTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCT                                                                         <  SRR4302533.6245901/94‑1 (MQ=60)

                       TGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTT                                                                        >  SRR4302533.1747427/1‑94 (MQ=60)

                         GGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAA                                                                      <  SRR4302533.6226530/94‑1 (MQ=60)

                          GTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAC                                                                     >  SRR4302533.4774669/1‑94 (MQ=60)

                          GTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAC                                                                     >  SRR4302533.4774844/1‑94 (MQ=60)

                          GTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAAC                                                                     >  SRR4302533.6332110/1‑94 (MQ=60)

                           TAAACAAAAAATCCGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTCCACGGGATAACGGGTTTTTGATATCTTAACC                                                                    <  SRR4302533.1931707/94‑1 (MQ=60)

                           TAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAAGGGGTTTTTGATATCTTAGCC                                                                    <  SRR4302533.3596397/94‑1 (MQ=60)

                           TAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACC                                                                    >  SRR4302533.751271/1‑94 (MQ=60)

                            AAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCG                                                                   >  SRR4302533.101529/1‑94 (MQ=60)

                            AAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCG                                                                   >  SRR4302533.5820383/1‑94 (MQ=60)

                               CAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACC                                                                <  SRR4302533.133873/94‑1 (MQ=60)

                               CAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACC                                                                <  SRR4302533.3636000/94‑1 (MQ=60)

                                AAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCT                                                               >  SRR4302533.1758510/1‑94 (MQ=60)

                                  AAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCG                                                             <  SRR4302533.1278121/94‑1 (MQ=60)

                                   AAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGG                                                            <  SRR4302533.5286667/94‑1 (MQ=60)

                                   AAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGG                                                            <  SRR4302533.5155680/94‑1 (MQ=60)

                                     ATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCC                                                          >  SRR4302533.842183/1‑94 (MQ=60)

                                     ATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCC                                                          >  SRR4302533.2338496/1‑94 (MQ=60)

                                       ACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTT                                                        <  SRR4302533.68447/94‑1 (MQ=60)

                                       ACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTT                                                        >  SRR4302533.4407504/1‑94 (MQ=60)

                                         GGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGA                                                      >  SRR4302533.3883703/1‑94 (MQ=60)

                                          GCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGAT                                                     <  SRR4302533.5647632/94‑1 (MQ=60)

                                          GCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGAT                                                     <  SRR4302533.2793674/94‑1 (MQ=60)

                                           CCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATG                                                    >  SRR4302533.3551249/1‑94 (MQ=60)

                                           CCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATG                                                    >  SRR4302533.3069245/1‑94 (MQ=60)

                                           CCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATG                                                    >  SRR4302533.2470108/1‑94 (MQ=60)

                                             CCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGT                                                  <  SRR4302533.4492928/94‑1 (MQ=60)

                                             CCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGT                                                  <  SRR4302533.2221096/94‑1 (MQ=60)

                                              CAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTC                                                 <  SRR4302533.1634376/94‑1 (MQ=60)

                                              CAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTC                                                 >  SRR4302533.3503228/1‑94 (MQ=60)

                                              CAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTC                                                 >  SRR4302533.4270258/1‑94 (MQ=60)

                                              CAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTC                                                 >  SRR4302533.1793725/1‑94 (MQ=60)

                                               AGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCG                                                >  SRR4302533.3031549/1‑94 (MQ=60)

                                               AGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCG                                                >  SRR4302533.5905538/1‑94 (MQ=60)

                                               AGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCG                                                <  SRR4302533.1664052/94‑1 (MQ=60)

                                               AGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCG                                                >  SRR4302533.2460209/1‑94 (MQ=60)

                                                GAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGG                                               <  SRR4302533.1408117/94‑1 (MQ=60)

                                                 AAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGG                                              >  SRR4302533.4541054/1‑94 (MQ=60)

                                                 AAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGG                                              >  SRR4302533.3623597/1‑94 (MQ=60)

                                                  AGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGG                                             >  SRR4302533.4564529/1‑94 (MQ=60)

                                                  AGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGG                                             <  SRR4302533.1669783/94‑1 (MQ=60)

                                                  AGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGG                                             <  SRR4302533.1945132/94‑1 (MQ=60)

                                                   GGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGG                                            <  SRR4302533.351715/94‑1 (MQ=60)

                                                   GGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGG                                            <  SRR4302533.5866085/94‑1 (MQ=60)

                                                   GGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGG                                            >  SRR4302533.1783862/1‑94 (MQ=60)

                                                      CAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGT                                         >  SRR4302533.836274/1‑94 (MQ=60)

                                                      CAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGT                                         >  SRR4302533.2154351/1‑94 (MQ=60)

                                                       AATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTT                                        >  SRR4302533.426833/1‑94 (MQ=60)

                                                          GCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTG                                     <  SRR4302533.2803944/94‑1 (MQ=60)

                                                          GCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTG                                     <  SRR4302533.5937160/94‑1 (MQ=60)

                                                          GCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTG                                     >  SRR4302533.3875556/1‑94 (MQ=60)

                                                            CGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTT                                   <  SRR4302533.6026007/94‑1 (MQ=60)

                                                            CGTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTT                                   >  SRR4302533.5421/1‑94 (MQ=60)

                                                             GTTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTA                                  <  SRR4302533.5021729/94‑1 (MQ=60)

                                                              TTCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTAT                                 >  SRR4302533.2979181/1‑94 (MQ=60)

                                                               TCACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATG                                >  SRR4302533.5989512/1‑94 (MQ=60)

                                                                CACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGA                               <  SRR4302533.1604233/94‑1 (MQ=60)

                                                                 ACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAA                              >  SRR4302533.3419313/1‑94 (MQ=60)

                                                                 ACTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAA                              <  SRR4302533.2332012/94‑1 (MQ=60)

                                                                  CTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAC                             <  SRR4302533.2324259/94‑1 (MQ=60)

                                                                  CTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAC                             <  SRR4302533.3774324/94‑1 (MQ=60)

                                                                  CTTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAAC                             <  SRR4302533.312366/94‑1 (MQ=60)

                                                                   TTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACA                            >  SRR4302533.850549/1‑94 (MQ=60)

                                                                   TTAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACA                            >  SRR4302533.1192975/1‑94 (MQ=60)

                                                                    TAAGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACAC                           >  SRR4302533.1329735/1‑94 (MQ=60)

                                                                      AGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCA                         >  SRR4302533.5918062/1‑94 (MQ=60)

                                                                      AGAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCA                         >  SRR4302533.439208/1‑94 (MQ=60)

                                                                       GAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCAC                        <  SRR4302533.2440864/94‑1 (MQ=60)

                                                                       GAGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCAC                        >  SRR4302533.4730993/1‑94 (MQ=60)

                                                                        AGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACT                       <  SRR4302533.3018938/94‑1 (MQ=60)

                                                                        AGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACT                       <  SRR4302533.5752010/94‑1 (MQ=60)

                                                                        AGGAGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACT                       <  SRR4302533.4876566/94‑1 (MQ=60)

                                                                           AGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCC                    >  SRR4302533.377504/1‑94 (MQ=60)

                                                                           AGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCC                    <  SRR4302533.4647044/94‑1 (MQ=60)

                                                                           AGCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCC                    >  SRR4302533.2404824/1‑94 (MQ=60)

                                                                            GCGGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCA                   <  SRR4302533.4408363/94‑1 (MQ=60)

                                                                              GGCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGA                 <  SRR4302533.874304/94‑1 (MQ=60)

                                                                               GCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAG                <  SRR4302533.2301934/94‑1 (MQ=60)

                                                                               GCACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAG                <  SRR4302533.4775407/94‑1 (MQ=60)

                                                                                CACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGC               >  SRR4302533.2180187/1‑94 (MQ=60)

                                                                                CACTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGC               <  SRR4302533.5894682/94‑1 (MQ=60)

                                                                                  CTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCAC             <  SRR4302533.3873263/94‑1 (MQ=60)

                                                                                  CTATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCAC             >  SRR4302533.3357919/1‑94 (MQ=60)

                                                                                   TATGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACC            >  SRR4302533.315801/1‑94 (MQ=60)

                                                                                     TGTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCC          <  SRR4302533.16002/94‑1 (MQ=60)

                                                                                      GTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCG         <  SRR4302533.3186776/94‑1 (MQ=60)

                                                                                      GTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCG         <  SRR4302533.5556535/94‑1 (MQ=60)

                                                                                      GTTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCG         <  SRR4302533.5428802/94‑1 (MQ=60)

                                                                                       TTTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGA        >  SRR4302533.2949887/1‑94 (MQ=60)

                                                                                        TTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAA       >  SRR4302533.4045314/1‑94 (MQ=60)

                                                                                        TTCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAA       <  SRR4302533.4978459/94‑1 (MQ=60)

                                                                                         TCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAG      >  SRR4302533.201418/1‑94 (MQ=60)

                                                                                         TCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAG      >  SRR4302533.2692117/1‑94 (MQ=60)

                                                                                         TCACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAG      >  SRR4302533.1835707/1‑94 (MQ=60)

                                                                                          CACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGA     >  SRR4302533.1794852/1‑94 (MQ=60)

                                                                                          CACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGA     <  SRR4302533.5640740/94‑1 (MQ=60)

                                                                                           ACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGAT    <  SRR4302533.3278339/94‑1 (MQ=60)

                                                                                           ACGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGAT    <  SRR4302533.5233461/94‑1 (MQ=60)

                                                                                            CGGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATG   >  SRR4302533.2550433/1‑94 (MQ=60)

                                                                                             GGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGC  >  SRR4302533.1476162/1‑94 (MQ=60)

GTCGGTTTATGGAAAAGTTGCTTTGGGTAAACAAAAAATACGGCCCCAGAAGGGCAATGCCGTTCACTTAAGAGGAGCGGCACTATGTTTCACAGGATAACGGGTTTTTGATATCTTAACCGACCTCGGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGC  >  NC_000913/1293586‑1293772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`