Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,961	A→G	E58E (GAA→GAG)	oppF →	OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,961	0	A	G	99.5%	641.9 / NA	201	E58E (GAA→GAG)	oppF	OppF
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (105/95); total (105/96)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTT  >  NC_000913/1303871‑1304050

 tGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCg                                                                                        >  1:3948345/1‑93 (MQ=255)

 tGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCg                                                                                        <  2:3258152/93‑1 (MQ=255)

   gTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCggg                                                                                      <  2:2605908/93‑1 (MQ=255)

   gTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCggg                                                                                      >  2:1442/1‑93 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     <  2:5761798/93‑1 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     >  1:5340407/1‑93 (MQ=255)

    tgcTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     <  2:1093664/91‑1 (MQ=255)

     tCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGAt                                                                                    >  1:2477466/1‑93 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  <  1:5307251/93‑1 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  >  1:2420177/1‑93 (MQ=255)

        ggCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCg                                                                                 >  1:5044423/1‑93 (MQ=255)

           aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTa                                                                              >  1:4833243/1‑93 (MQ=255)

           aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTa                                                                              >  2:5011867/1‑93 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                             >  1:62937/1‑93 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                             >  2:5143499/1‑93 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                             <  1:1737277/93‑1 (MQ=255)

             ccgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAg                                                                            >  1:3416305/1‑93 (MQ=255)

             ccgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAg                                                                            <  2:395999/93‑1 (MQ=255)

               gccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTc                                                                          <  2:3616402/93‑1 (MQ=255)

               gccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTc                                                                          >  1:1939275/1‑93 (MQ=255)

                ccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTcc                                                                         >  1:4557637/1‑93 (MQ=255)

                 cgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCa                                                                        >  1:2688882/1‑93 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGATACATTAGGTGTGGTAGGGGAGTCGGGGTGCGGTAAGTCCAc                                                                       >  1:361334/1‑93 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCGAc                                                                       >  1:5445333/1‑93 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAc                                                                       <  2:1575775/93‑1 (MQ=255)

                   aaaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAcc                                                                      >  1:5569058/1‑93 (MQ=255)

                   aaaaCGCTCAAAGCCGTCGATGGTCTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAcc                                                                      <  2:101337/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                    <  1:4177727/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                    <  1:4425824/93‑1 (MQ=255)

                       cGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTg                                                                  >  1:2097033/1‑93 (MQ=255)

                       cGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTg                                                                  <  2:2325545/93‑1 (MQ=255)

                       cGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTg                                                                  <  1:3185487/93‑1 (MQ=255)

                        gCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGc                                                                 <  2:4780887/93‑1 (MQ=255)

                        gCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGc                                                                 <  2:488135/93‑1 (MQ=255)

                         cTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGCAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCt                                                                >  2:3413963/1‑93 (MQ=255)

                         cTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGTGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCt                                                                >  2:3058632/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  2:1717497/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               <  2:2427958/93‑1 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  1:3288421/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  1:3144588/1‑93 (MQ=255)

                           cAAAGCCGACGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcg                                                              >  2:6130197/1‑93 (MQ=255)

                           cAAAGCCATCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcg                                                              >  1:1644642/1‑93 (MQ=255)

                            aaaGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcgc                                                             >  2:6201099/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTCTGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        >  2:5210635/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        >  2:6180403/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        >  2:2060778/1‑93 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                       <  2:381315/93‑1 (MQ=255)

                                   tCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                      <  1:5766515/93‑1 (MQ=255)

                                   tCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                      >  2:1915116/1‑93 (MQ=255)

                                     gATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCatcatc                                                    >  2:2473863/1‑93 (MQ=255)

                                      aTGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCg                                                   >  1:5897035/1‑93 (MQ=255)

                                       tGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCgg                                                  >  2:4059512/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 <  1:2983682/93‑1 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  2:36017/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  1:364005/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  2:5447704/1‑93 (MQ=255)

                                         gtgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGtt                                                <  2:337002/93‑1 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                             >  1:3484378/1‑93 (MQ=255)

                                             cACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGt                                            <  2:2367865/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         <  2:5884798/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         <  2:3557616/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAAAATTAGGTGGGGTAGGGGAGTCGGGCTGCGGCAAGTCCACCTTTGCTCGCGCCATCATCGGTTTggctaa                                         >  2:1799453/1‑89 (MQ=255)

                                                  ttCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAgg                                       <  2:299915/93‑1 (MQ=255)

                                                     gCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcga                                    <  2:3670294/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                   >  1:4495405/1‑93 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                   >  2:3174909/1‑93 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGAGTGGTAGGGGAGTCGGGATGCGGTAAGTTCACCTTTGCTCGCGCCATCATCGGGTTGGTCAACGcgac                                   >  2:6014776/1‑93 (MQ=255)

                                                       cTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgacc                                  >  1:1420191/1‑93 (MQ=255)

                                                        tGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccg                                 >  1:5966862/1‑93 (MQ=255)

                                                         gTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                <  2:4986805/93‑1 (MQ=255)

                                                         gTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                <  2:1150013/93‑1 (MQ=255)

                                                         gTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                >  1:5112389/1‑93 (MQ=255)

                                                          tATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccgac                               >  1:3255459/1‑93 (MQ=255)

                                                           aTGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACg                              <  2:2280645/93‑1 (MQ=255)

                                                            tGAAGGGGAAACCGTAGGCGTGGTAGGGGAGCCGGGCTGCGGGAAGTTCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  2:5634076/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  1:816028/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  1:4024549/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  1:718582/1‑93 (MQ=255)

                                                               aGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGGTTGGTGAAGGCGACCGACGGTCa                          >  2:1203469/1‑93 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                         >  1:5535801/1‑93 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                       <  1:376197/93‑1 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                      >  1:3454952/1‑93 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                      >  2:148759/1‑93 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                      <  2:4967731/93‑1 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                   >  2:448522/1‑93 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                   <  2:5188923/93‑1 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                 <  1:1182229/93‑1 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGAGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                 <  2:4342890/93‑1 (MQ=255)

                                                                            ggTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa             <  1:4906928/93‑1 (MQ=255)

                                                                            ggTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa             <  2:213432/93‑1 (MQ=255)

                                                                             gtgtGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAg            <  1:1198628/93‑1 (MQ=255)

                                                                              tgtgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAgg           >  1:2558043/1‑93 (MQ=255)

                                                                                tgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTa         <  1:3014331/93‑1 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  1:4632486/1‑93 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  2:4400572/1‑93 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  1:891356/1‑93 (MQ=255)

                                                                                    aGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAaga     <  2:285367/93‑1 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag    >  2:1987651/1‑93 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag    >  2:5849919/1‑93 (MQ=255)

                                                                                       ggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGtt  <  2:2226470/93‑1 (MQ=255)

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTT  >  NC_000913/1303871‑1304050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTG  >  NC_000913/1303868‑1304054

ACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAG                                                                                               <  SRR4302533.3034489/94‑1 (MQ=60)

 CAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGT                                                                                              >  SRR4302533.4303438/1‑94 (MQ=60)

   GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCG                                                                                            <  SRR4302533.918471/94‑1 (MQ=60)

   GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCG                                                                                            >  SRR4302533.3972422/1‑94 (MQ=60)

      GTTCTGGCACCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGA                                                                                         <  SRR4302533.3353997/94‑1 (MQ=60)

      GTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGA                                                                                         >  SRR4302533.5372432/1‑94 (MQ=60)

       TTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGAT                                                                                        >  SRR4302533.6150248/1‑94 (MQ=60)

       TTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGAT                                                                                        >  SRR4302533.2492748/1‑94 (MQ=60)

        TCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATG                                                                                       <  SRR4302533.3574509/94‑1 (MQ=60)

        TCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATG                                                                                       <  SRR4302533.3368860/94‑1 (MQ=60)

        TCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATG                                                                                       <  SRR4302533.2363818/94‑1 (MQ=60)

         CTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGC                                                                                      >  SRR4302533.2435124/1‑94 (MQ=60)

          TGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCG                                                                                     >  SRR4302533.5074946/1‑94 (MQ=60)

          TGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCG                                                                                     <  SRR4302533.5339059/94‑1 (MQ=60)

           GGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGG                                                                                    >  SRR4302533.4686031/1‑94 (MQ=60)

           GGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGG                                                                                    <  SRR4302533.1771302/94‑1 (MQ=60)

             CAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTA                                                                                  >  SRR4302533.4862540/1‑94 (MQ=60)

             CAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTA                                                                                  >  SRR4302533.3497808/1‑94 (MQ=60)

              AACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAA                                                                                 >  SRR4302533.63350/1‑94 (MQ=60)

              AACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAA                                                                                 <  SRR4302533.5340464/94‑1 (MQ=60)

               ACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAG                                                                                >  SRR4302533.3437411/1‑94 (MQ=60)

               ACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAG                                                                                <  SRR4302533.1747818/94‑1 (MQ=60)

                 CGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTC                                                                              >  SRR4302533.1951103/1‑94 (MQ=60)

                 CGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTC                                                                              >  SRR4302533.2949508/1‑94 (MQ=60)

                  GCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCC                                                                             >  SRR4302533.1477589/1‑94 (MQ=60)

                  GCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCC                                                                             >  SRR4302533.4585275/1‑94 (MQ=60)

                   CCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCA                                                                            >  SRR4302533.2705367/1‑94 (MQ=60)

                    CGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGATACATTAGGTGTGGTAGGGGAGTCGGGGTGCGGTAAGTCCAC                                                                           >  SRR4302533.363530/1‑94 (MQ=60)

                    CGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCGAC                                                                           >  SRR4302533.5478141/1‑94 (MQ=60)

                    CGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAC                                                                           <  SRR4302533.4917459/94‑1 (MQ=60)

                     GAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACC                                                                          >  SRR4302533.5602721/1‑94 (MQ=60)

                        AACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTT                                                                       <  SRR4302533.4203039/94‑1 (MQ=60)

                        AACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTT                                                                       <  SRR4302533.4452575/94‑1 (MQ=60)

                        AACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTT                                                                       <  SRR4302533.3268304/94‑1 (MQ=60)

                         ACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTG                                                                      >  SRR4302533.2109943/1‑94 (MQ=60)

                         ACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTG                                                                      <  SRR4302533.2106519/94‑1 (MQ=60)

                          CGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGC                                                                     <  SRR4302533.3205079/94‑1 (MQ=60)

                            CTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTC                                                                   >  SRR4302533.3308720/1‑94 (MQ=60)

                            CTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTC                                                                   >  SRR4302533.5601248/1‑94 (MQ=60)

                            CTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTC                                                                   >  SRR4302533.3163923/1‑94 (MQ=60)

                             TCAAAGCCATCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCG                                                                  >  SRR4302533.1654600/1‑94 (MQ=60)

                              CAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGC                                                                 <  SRR4302533.5806586/94‑1 (MQ=60)

                              CAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGC                                                                 >  SRR4302533.876595/1‑94 (MQ=60)

                               AAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCG                                                                >  SRR4302533.3301319/1‑94 (MQ=60)

                               AAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCG                                                                <  SRR4302533.4543065/94‑1 (MQ=60)

                                    CGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATC                                                           <  SRR4302533.5967666/94‑1 (MQ=60)

                                     GTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCA                                                          >  SRR4302533.5373779/1‑94 (MQ=60)

                                      TCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCAT                                                         <  SRR4302533.5801328/94‑1 (MQ=60)

                                       CGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATC                                                        >  SRR4302533.4030595/1‑94 (MQ=60)

                                        GATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCG                                                       >  SRR4302533.5932645/1‑94 (MQ=60)

                                          TGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGT                                                     >  SRR4302533.516290/1‑94 (MQ=60)

                                          TGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGT                                                     >  SRR4302533.366214/1‑94 (MQ=60)

                                           GGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTT                                                    <  SRR4302533.3001932/94‑1 (MQ=60)

                                            GTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTT                                                   >  SRR4302533.347050/1‑94 (MQ=60)

                                            GTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGATAAGTCCACCTTTGCTCGCGCCATCATCGGTTT                                                   <  SRR4302533.4956643/94‑1 (MQ=60)

                                              GTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGG                                                 >  SRR4302533.3505866/1‑94 (MQ=60)

                                               TCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGT                                                <  SRR4302533.5514961/94‑1 (MQ=60)

                                                 ACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCA                                              >  SRR4302533.2608660/1‑94 (MQ=60)

                                                   TCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAG                                            <  SRR4302533.3644257/94‑1 (MQ=60)

                                                    CTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGG                                           <  SRR4302533.4253073/94‑1 (MQ=60)

                                                    CTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGG                                           >  SRR4302533.1377910/1‑94 (MQ=60)

                                                        GCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGAC                                       >  SRR4302533.4522627/1‑94 (MQ=60)

                                                         CCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACC                                      >  SRR4302533.1428783/1‑94 (MQ=60)

                                                         CCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACC                                      <  SRR4302533.4128310/94‑1 (MQ=60)

                                                          CTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCG                                     >  SRR4302533.6002984/1‑94 (MQ=60)

                                                          CTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCG                                     <  SRR4302533.167520/94‑1 (MQ=60)

                                                          CTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCG                                     >  SRR4302533.2457460/1‑94 (MQ=60)

                                                           TGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGA                                    >  SRR4302533.5143282/1‑94 (MQ=60)

                                                            GTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGAC                                   >  SRR4302533.3275535/1‑94 (MQ=60)

                                                              ATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGG                                 >  SRR4302533.722824/1‑94 (MQ=60)

                                                              ATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGG                                 >  SRR4302533.820811/1‑94 (MQ=60)

                                                              ATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGG                                 >  SRR4302533.4049049/1‑94 (MQ=60)

                                                                GAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTC                               <  SRR4302533.3782349/94‑1 (MQ=60)

                                                                GAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTC                               <  SRR4302533.1190120/94‑1 (MQ=60)

                                                                 AAGGGGAAACATTAGGTGTGGTAGGGTAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCA                              >  SRR4302533.3098122/1‑94 (MQ=60)

                                                                  AGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAT                             >  SRR4302533.5569226/1‑94 (MQ=60)

                                                                     GGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTT                          <  SRR4302533.378467/94‑1 (MQ=60)

                                                                     GGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTT                          >  SRR4302533.4652474/1‑94 (MQ=60)

                                                                     GGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTT                          >  SRR4302533.3476277/1‑94 (MQ=60)

                                                                      GAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTG                         <  SRR4302533.3869845/94‑1 (MQ=60)

                                                                      GAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTG                         >  SRR4302533.5285889/1‑94 (MQ=60)

                                                                         ACATTAGGTGTGGTAGGGGAGTCGGGATACGGTAAGTCCACCTTAGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCT                      <  SRR4302533.6336297/94‑1 (MQ=60)

                                                                          CATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTACCTG                     >  SRR4302533.4454004/1‑94 (MQ=60)

                                                                           ATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGG                    <  SRR4302533.1189331/94‑1 (MQ=60)

                                                                           ATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGG                    >  SRR4302533.5084126/1‑94 (MQ=60)

                                                                             TAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTT                  >  SRR4302533.5780295/1‑94 (MQ=60)

                                                                               GGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAG                <  SRR4302533.4936657/94‑1 (MQ=60)

                                                                                GTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGG               <  SRR4302533.3014756/94‑1 (MQ=60)

                                                                                GTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGG               >  SRR4302533.2573770/1‑94 (MQ=60)

                                                                                GTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGG               <  SRR4302533.1205829/94‑1 (MQ=60)

                                                                                   TGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAA            <  SRR4302533.3032766/94‑1 (MQ=60)

                                                                                    GGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAA           >  SRR4302533.4660560/1‑94 (MQ=60)

                                                                                    GGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAA           >  SRR4302533.896594/1‑94 (MQ=60)

                                                                                       AGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAG        <  SRR4302533.2526794/94‑1 (MQ=60)

                                                                                          GGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTG     <  SRR4302533.3182137/94‑1 (MQ=60)

                                                                                          GGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTG     >  SRR4302533.5785214/1‑94 (MQ=60)

                                                                                          GGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTG     <  SRR4302533.817249/94‑1 (MQ=60)

                                                                                           GAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGC    <  SRR4302533.4967070/94‑1 (MQ=60)

                                                                                           GAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGC    >  SRR4302533.2277180/1‑94 (MQ=60)

                                                                                           GAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGC    <  SRR4302533.5682718/94‑1 (MQ=60)

                                                                                             GTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTG  >  SRR4302533.5330461/1‑94 (MQ=60)

ACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTG  >  NC_000913/1303868‑1304054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`