Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,461,874	T→C	P104P (CCT→CCC)	b1399 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,461,874	0	T	C	100.0%	746.4 / NA	232	P104P (CCT→CCC)	b1399	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (121/111); total (121/111)

Only 100 of 239 total aligned reads displayed.

CATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGG  >  NC_000913/1461784‑1461963

 aTCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCa                                                                                        <  1:494515/93‑1 (MQ=255)

  tCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCAt                                                                                       >  1:1078869/1‑93 (MQ=255)

  tCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCAt                                                                                       >  2:2908652/1‑93 (MQ=255)

   cGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATg                                                                                      <  1:2067913/93‑1 (MQ=255)

    gggCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATgg                                                                                     >  2:2528735/1‑93 (MQ=255)

    gggCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATgg                                                                                     <  2:4047816/93‑1 (MQ=255)

     ggCGACGCTGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATggg                                                                                    >  2:6135475/1‑93 (MQ=255)

     ggCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATggg                                                                                    >  2:1248124/1‑93 (MQ=255)

     ggCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATggg                                                                                    >  1:2340206/1‑93 (MQ=255)

      gCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCCGCGGAACCCGCATGGGa                                                                                   >  2:6245105/1‑93 (MQ=255)

      gCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGa                                                                                   >  2:2641327/1‑93 (MQ=255)

       cGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGAt                                                                                  <  1:654287/93‑1 (MQ=255)

          cGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGt                                                                               <  2:3477714/93‑1 (MQ=255)

            cAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTaa                                                                             >  2:3169761/1‑93 (MQ=255)

             aGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTaaa                                                                            >  2:2194660/1‑93 (MQ=255)

              gTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAAt                                                                           <  1:5717125/93‑1 (MQ=255)

                ttCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATgg                                                                         <  1:1123079/93‑1 (MQ=255)

                ttCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATgg                                                                         >  1:2979956/1‑93 (MQ=255)

                 tCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGc                                                                        <  1:5152347/93‑1 (MQ=255)

                   tataGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTc                                                                      <  2:1588849/93‑1 (MQ=255)

                    ataGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTcc                                                                     <  2:6191187/93‑1 (MQ=255)

                     taGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCt                                                                    >  1:3281256/1‑93 (MQ=255)

                      aGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTg                                                                   <  1:3679542/93‑1 (MQ=255)

                       gCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCCTGGGGTGGTAAATGGCTCCTGt                                                                  >  1:3806821/1‑93 (MQ=255)

                       gCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGt                                                                  >  1:1878666/1‑93 (MQ=255)

                       gCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGt                                                                  >  1:1006468/1‑93 (MQ=255)

                       gCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGt                                                                  <  1:2447431/93‑1 (MQ=255)

                       gCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGt                                                                  >  1:1619626/1‑93 (MQ=255)

                       gCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGt                                                                  <  1:389208/93‑1 (MQ=255)

                          tCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCTCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGc                                                               <  2:1286423/93‑1 (MQ=255)

                          tCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGc                                                               >  1:5558680/1‑93 (MQ=255)

                          tCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGc                                                               >  2:5261592/1‑93 (MQ=255)

                            aGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGctc                                                             <  2:2265124/93‑1 (MQ=255)

                             gTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGctct                                                            >  1:5649564/1‑93 (MQ=255)

                               gATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCg                                                          <  1:1388152/93‑1 (MQ=255)

                                aTAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCgg                                                         <  1:3589778/93‑1 (MQ=255)

                                 tAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCTGa                                                        >  2:5808053/1‑93 (MQ=255)

                                 tAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGa                                                        >  1:4056439/1‑93 (MQ=255)

                                  aaaGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGaa                                                       >  1:3076777/1‑93 (MQ=255)

                                   aaGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAg                                                      <  1:3326239/93‑1 (MQ=255)

                                   aaGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAg                                                      >  1:5771051/1‑93 (MQ=255)

                                   aaGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAg                                                      >  1:767651/1‑93 (MQ=255)

                                     ggCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCGTGTTGCTCTCGGAAGGt                                                    <  2:5994645/93‑1 (MQ=255)

                                      gCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGtt                                                   <  2:433204/93‑1 (MQ=255)

                                        ttGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTa                                                 <  1:2546802/93‑1 (MQ=255)

                                         tGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAg                                                >  2:4615733/1‑93 (MQ=255)

                                           cgcCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGAt                                              >  2:3494650/1‑93 (MQ=255)

                                            gcCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATa                                             <  1:843995/93‑1 (MQ=255)

                                             ccTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATaa                                            >  1:1699658/1‑93 (MQ=255)

                                             ccTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATaa                                            <  2:4472441/93‑1 (MQ=255)

                                             ccTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATaa                                            <  1:2308130/93‑1 (MQ=255)

                                              cTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATaaa                                           >  2:3922471/1‑93 (MQ=255)

                                              cTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATaaa                                           <  2:4660059/93‑1 (MQ=255)

                                                 aCGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCa                                        >  1:3959112/1‑93 (MQ=255)

                                                  cgcgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCaa                                       <  2:4454785/93‑1 (MQ=255)

                                                  cgcgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCaa                                       <  1:2605460/93‑1 (MQ=255)

                                                   gcgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAAc                                      >  2:4962148/1‑93 (MQ=255)

                                                   gcgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAAc                                      <  1:1465242/93‑1 (MQ=255)

                                                   gcgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAAc                                      <  2:5951083/93‑1 (MQ=255)

                                                    cgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACg                                     <  2:407793/93‑1 (MQ=255)

                                                     gACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACgc                                    >  1:437139/1‑93 (MQ=255)

                                                     gACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACgc                                    <  2:176102/93‑1 (MQ=255)

                                                      aCGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACgct                                   <  2:1965677/93‑1 (MQ=255)

                                                      aCGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACgct                                   <  1:4069309/93‑1 (MQ=255)

                                                       cGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACgctg                                  <  2:572462/93‑1 (MQ=255)

                                                         ggCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACgctggc                                >  2:2744841/1‑93 (MQ=255)

                                                            aGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGa                             >  2:4099296/1‑93 (MQ=255)

                                                            aGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGa                             <  1:2514767/93‑1 (MQ=255)

                                                             gAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAt                            >  2:3646313/1‑93 (MQ=255)

                                                             gAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAt                            <  2:6049730/93‑1 (MQ=255)

                                                             gAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAt                            >  1:5879042/1‑93 (MQ=255)

                                                             gAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAt                            >  1:3213161/1‑93 (MQ=255)

                                                             gAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAt                            >  1:3884250/1‑93 (MQ=255)

                                                              aaaGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATg                           >  1:3041120/1‑93 (MQ=255)

                                                                 gTAAAATTTATCGCGCCGAGCAAACCGCCTGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATAAACGCTGGCTGATGTCa                        >  2:1169847/1‑93 (MQ=255)

                                                                  tAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCaa                       >  1:6180721/1‑93 (MQ=255)

                                                                    aaaTTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCaaaa                     >  1:4515356/1‑93 (MQ=255)

                                                                      aTTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAg                   <  1:1197878/93‑1 (MQ=255)

                                                                       tttATTGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGc                  <  1:3395949/93‑1 (MQ=255)

                                                                        ttATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCa                 <  2:5377089/93‑1 (MQ=255)

                                                                         tATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAg                <  1:4305111/93‑1 (MQ=255)

                                                                          aTCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGt               >  2:643741/1‑93 (MQ=255)

                                                                          aTCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGt               <  1:2908652/93‑1 (MQ=255)

                                                                           tCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGGTGATGTCAAAAAGCCGtt              >  1:5454643/1‑93 (MQ=255)

                                                                           tCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGtt              >  2:3134755/1‑93 (MQ=255)

                                                                           tCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGtt              >  2:2997252/1‑93 (MQ=255)

                                                                           tCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGtt              <  2:371172/93‑1 (MQ=255)

                                                                           tCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGtt              >  1:5534814/1‑93 (MQ=255)

                                                                               gcAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATc          <  1:5052228/93‑1 (MQ=255)

                                                                               gcAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATc          <  1:2665208/93‑1 (MQ=255)

                                                                               gcAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATc          >  1:4149144/1‑93 (MQ=255)

                                                                                cAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCt         >  2:5436482/1‑93 (MQ=255)

                                                                                cAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCt         <  1:2002087/93‑1 (MQ=255)

                                                                                  gagCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTgg       >  2:2712109/1‑93 (MQ=255)

                                                                                   agCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGc      >  2:576474/1‑93 (MQ=255)

                                                                                    gCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCa     <  1:4548073/93‑1 (MQ=255)

                                                                                     cAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCag    >  1:5819632/1‑92 (MQ=255)

                                                                                      aaCCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAg   <  2:5015409/93‑1 (MQ=255)

                                                                                       aCCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAgg  >  2:1051884/1‑93 (MQ=255)

                                                                                       aCCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAgg  >  1:5363066/1‑93 (MQ=255)

CATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGG  >  NC_000913/1461784‑1461963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGCATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTT  >  NC_000913/1461781‑1461967

CCGCATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCC                                                                                               >  SRR4302533.717964/1‑94 (MQ=60)

   CATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCA                                                                                            >  SRR4302533.4169647/1‑94 (MQ=60)

    ATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCAT                                                                                           >  SRR4302533.1085228/1‑94 (MQ=60)

    ATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCAT                                                                                           <  SRR4302533.1139774/94‑1 (MQ=60)

    ATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCAT                                                                                           <  SRR4302533.497464/94‑1 (MQ=60)

      CGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGG                                                                                         <  SRR4302533.2080653/94‑1 (MQ=60)

      CGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGG                                                                                         >  SRR4302533.4555212/1‑94 (MQ=60)

       GGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGG                                                                                        >  SRR4302533.2354697/1‑94 (MQ=60)

          CGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATG                                                                                     <  SRR4302533.4723275/94‑1 (MQ=60)

          CGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATG                                                                                     <  SRR4302533.658148/94‑1 (MQ=60)

           GACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGG                                                                                    <  SRR4302533.130487/94‑1 (MQ=60)

              GCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAA                                                                                 >  SRR4302533.4031001/1‑94 (MQ=60)

              GCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGGAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAA                                                                                 >  SRR4302533.2887246/1‑94 (MQ=60)

               CAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAA                                                                                <  SRR4302533.4174458/94‑1 (MQ=60)

                AGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAAT                                                                               <  SRR4302533.3951515/94‑1 (MQ=60)

                 GTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATG                                                                              <  SRR4302533.5751653/94‑1 (MQ=60)

                 GTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATG                                                                              >  SRR4302533.3590561/1‑94 (MQ=60)

                  TTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGG                                                                             >  SRR4302533.2998190/1‑94 (MQ=60)

                   TTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGC                                                                            >  SRR4302533.5730402/1‑94 (MQ=60)

                   TTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGC                                                                            <  SRR4302533.1129744/94‑1 (MQ=60)

                   TTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGC                                                                            >  SRR4302533.2267720/1‑94 (MQ=60)

                    TCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCT                                                                           <  SRR4302533.5183470/94‑1 (MQ=60)

                     CTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTC                                                                          >  SRR4302533.3069637/1‑94 (MQ=60)

                     CTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGGAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTC                                                                          >  SRR4302533.3073013/1‑94 (MQ=60)

                     CTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGAAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGCTGGTACATGGCTC                                                                          >  SRR4302533.2547343/1‑94 (MQ=60)

                       ATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAATTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCT                                                                        <  SRR4302533.1926044/94‑1 (MQ=60)

                       ATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCT                                                                        >  SRR4302533.3301512/1‑94 (MQ=60)

                       ATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCT                                                                        <  SRR4302533.234850/94‑1 (MQ=60)

                         AGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCCTGGGGTGGTAAATGGCTCCTGT                                                                      >  SRR4302533.3830112/1‑94 (MQ=60)

                         AGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGT                                                                      <  SRR4302533.3702158/94‑1 (MQ=60)

                         AGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGT                                                                      >  SRR4302533.3830113/1‑94 (MQ=60)

                         AGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGT                                                                      >  SRR4302533.1890115/1‑94 (MQ=60)

                         AGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGT                                                                      >  SRR4302533.1629439/1‑94 (MQ=60)

                         AGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGT                                                                      >  SRR4302533.1012356/1‑94 (MQ=60)

                          GCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTT                                                                     <  SRR4302533.391557/94‑1 (MQ=60)

                          GCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTT                                                                     <  SRR4302533.94018/94‑1 (MQ=60)

                          GCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTT                                                                     <  SRR4302533.2462534/94‑1 (MQ=60)

                            CTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGC                                                                   >  SRR4302533.5592276/1‑94 (MQ=60)

                             TCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCT                                                                  <  SRR4302533.2361396/94‑1 (MQ=60)

                               AGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCT                                                                >  SRR4302533.5359485/1‑94 (MQ=60)

                               AGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCT                                                                >  SRR4302533.5683715/1‑94 (MQ=60)

                                GTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTC                                                               <  SRR4302533.3201179/94‑1 (MQ=60)

                                 TGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCG                                                              <  SRR4302533.3301410/94‑1 (MQ=60)

                                 TGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCG                                                              >  SRR4302533.5574286/1‑94 (MQ=60)

                                  GATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGG                                                             >  SRR4302533.5368335/1‑94 (MQ=60)

                                  GATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGG                                                             <  SRR4302533.1396510/94‑1 (MQ=60)

                                   ATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGA                                                            <  SRR4302533.3611878/94‑1 (MQ=60)

                                   ATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGA                                                            >  SRR4302533.4081132/1‑94 (MQ=60)

                                    TAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAA                                                           >  SRR4302533.218049/1‑94 (MQ=60)

                                    TAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAA                                                           >  SRR4302533.3095660/1‑94 (MQ=60)

                                     AAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAG                                                          >  SRR4302533.5805898/1‑94 (MQ=60)

                                     AAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAG                                                          >  SRR4302533.4086265/1‑94 (MQ=60)

                                     AAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAG                                                          >  SRR4302533.772180/1‑94 (MQ=60)

                                      AAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGG                                                         <  SRR4302533.3346790/94‑1 (MQ=60)

                                      AAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGG                                                         >  SRR4302533.5510023/1‑94 (MQ=60)

                                       AGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGT                                                        <  SRR4302533.1514493/94‑1 (MQ=60)

                                        GGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGGGCAGAGCAACCCGCATGGGATGGGAAATGGCTCCTGTTGCTCTacggatgtt                                                       >  SRR4302533.4165837/1‑85 (MQ=60)

                                        GGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTT                                                       <  SRR4302533.5407570/94‑1 (MQ=60)

                                        GGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGGT                                                       >  SRR4302533.2522271/1‑94 (MQ=60)

                                         GCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTT                                                      <  SRR4302533.2446294/94‑1 (MQ=60)

                                          CTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTA                                                     <  SRR4302533.810264/94‑1 (MQ=60)

                                          CTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTA                                                     >  SRR4302533.3936529/1‑94 (MQ=60)

                                          CTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTA                                                     >  SRR4302533.5794437/1‑94 (MQ=60)

                                           TTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAG                                                    <  SRR4302533.2562472/94‑1 (MQ=60)

                                              CGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATA                                                 >  SRR4302533.4381108/1‑94 (MQ=60)

                                              CGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATA                                                 <  SRR4302533.583309/94‑1 (MQ=60)

                                               GCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAA                                                >  SRR4302533.1709959/1‑94 (MQ=60)

                                               GCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAA                                                <  SRR4302533.848929/94‑1 (MQ=60)

                                               GCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAA                                                >  SRR4302533.1830440/1‑94 (MQ=60)

                                               GCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAA                                                <  SRR4302533.5560572/94‑1 (MQ=60)

                                               GCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAA                                                <  SRR4302533.683528/94‑1 (MQ=60)

                                                CCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAA                                               <  SRR4302533.2322427/94‑1 (MQ=60)

                                                 CTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACGCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAAT                                              <  SRR4302533.3635052/94‑1 (MQ=60)

                                                 CTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAAT                                              <  SRR4302533.436613/94‑1 (MQ=60)

                                                   GACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCA                                            >  SRR4302533.3983239/1‑94 (MQ=60)

                                                    ACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAA                                           >  SRR4302533.2872754/1‑94 (MQ=60)

                                                    ACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAA                                           <  SRR4302533.5107959/94‑1 (MQ=60)

                                                     CGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAAC                                          <  SRR4302533.2621465/94‑1 (MQ=60)

                                                     CGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAAC                                          >  SRR4302533.2675341/1‑94 (MQ=60)

                                                      GCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACG                                         <  SRR4302533.1474078/94‑1 (MQ=60)

                                                       CGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGC                                        >  SRR4302533.439743/1‑94 (MQ=60)

                                                         ACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTG                                      <  SRR4302533.4094080/94‑1 (MQ=60)

                                                         ACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTG                                      >  SRR4302533.818418/1‑94 (MQ=60)

                                                          CGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGG                                     <  SRR4302533.1660942/94‑1 (MQ=60)

                                                          CGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGG                                     >  SRR4302533.3709506/1‑94 (MQ=60)

                                                            GGCAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCT                                   <  SRR4302533.141080/94‑1 (MQ=60)

                                                              CAGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGA                                 >  SRR4302533.334904/1‑94 (MQ=60)

                                                               AGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAT                                >  SRR4302533.3232964/1‑94 (MQ=60)

                                                               AGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAT                                <  SRR4302533.2530264/94‑1 (MQ=60)

                                                               AGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAT                                >  SRR4302533.3907950/1‑94 (MQ=60)

                                                               AGAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGAT                                >  SRR4302533.5914575/1‑94 (MQ=60)

                                                                GAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATG                               >  SRR4302533.4641248/1‑94 (MQ=60)

                                                                GAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATG                               >  SRR4302533.30080/1‑94 (MQ=60)

                                                                GAAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATG                               >  SRR4302533.3059734/1‑94 (MQ=60)

                                                                 AAAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGT                              >  SRR4302533.5973545/1‑94 (MQ=60)

                                                                  AAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTC                             >  SRR4302533.2593038/1‑94 (MQ=60)

                                                                  AAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTC                             >  SRR4302533.5032996/1‑94 (MQ=60)

                                                                  AAGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTC                             <  SRR4302533.2065995/94‑1 (MQ=60)

                                                                   AGTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCA                            <  SRR4302533.3518425/94‑1 (MQ=60)

                                                                    GTAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAA                           >  SRR4302533.6217839/1‑94 (MQ=60)

                                                                     TAAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAA                          >  SRR4302533.3331502/1‑94 (MQ=60)

                                                                      AAAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAA                         >  SRR4302533.4542697/1‑94 (MQ=60)

                                                                       AAATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAA                        <  SRR4302533.2951528/94‑1 (MQ=60)

                                                                        AATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAG                       >  SRR4302533.1579020/1‑94 (MQ=60)

                                                                         ATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGC                      <  SRR4302533.1205075/94‑1 (MQ=60)

                                                                         ATTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGC                      <  SRR4302533.1076609/94‑1 (MQ=60)

                                                                          TTTATTGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA                     <  SRR4302533.3416921/94‑1 (MQ=60)

                                                                          TTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA                     >  SRR4302533.5863156/1‑94 (MQ=60)

                                                                          TTTATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA                     <  SRR4302533.3324662/94‑1 (MQ=60)

                                                                            TATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGT                   <  SRR4302533.4331013/94‑1 (MQ=60)

                                                                             ATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGGTGATGTCAAAAAGCCGTT                  >  SRR4302533.5487501/1‑94 (MQ=60)

                                                                             ATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTT                  >  SRR4302533.5568229/1‑94 (MQ=60)

                                                                             ATCGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTT                  <  SRR4302533.2926507/94‑1 (MQ=60)

                                                                               CGCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGA                >  SRR4302533.5595091/1‑94 (MQ=60)

                                                                                GCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGAT               <  SRR4302533.2567055/94‑1 (MQ=60)

                                                                                GCGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGAT               <  SRR4302533.3909571/94‑1 (MQ=60)

                                                                                 CGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATC              >  SRR4302533.3868171/1‑94 (MQ=60)

                                                                                 CGCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATC              >  SRR4302533.4174283/1‑94 (MQ=60)

                                                                                  GCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCT             >  SRR4302533.1137933/1‑94 (MQ=60)

                                                                                  GCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCT             <  SRR4302533.2681564/94‑1 (MQ=60)

                                                                                  GCAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCT             <  SRR4302533.5082776/94‑1 (MQ=60)

                                                                                   CAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTG            >  SRR4302533.4140433/1‑94 (MQ=60)

                                                                                   CAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTG            >  SRR4302533.5747942/1‑94 (MQ=60)

                                                                                   CAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTG            <  SRR4302533.439780/94‑1 (MQ=60)

                                                                                   CAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTG            <  SRR4302533.2014415/94‑1 (MQ=60)

                                                                                   CAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTG            <  SRR4302533.6307817/94‑1 (MQ=60)

                                                                                   CAGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTG            <  SRR4302533.6307818/94‑1 (MQ=60)

                                                                                    AGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGG           >  SRR4302533.1107223/1‑94 (MQ=60)

                                                                                    AGAGCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGG           >  SRR4302533.582840/1‑94 (MQ=60)

                                                                                       GCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAG        >  SRR4302533.5854788/1‑94 (MQ=60)

                                                                                       GCAACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAA        <  SRR4302533.4575618/94‑1 (MQ=60)

                                                                                         AACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGG      >  SRR4302533.5395276/1‑94 (MQ=60)

                                                                                          ACCCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGT     <  SRR4302533.1849311/94‑1 (MQ=60)

                                                                                            CCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTT   >  SRR4302533.5762979/1‑94 (MQ=60)

                                                                                            CCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTT   >  SRR4302533.791529/1‑94 (MQ=60)

                                                                                            CCGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTT   <  SRR4302533.3312121/94‑1 (MQ=60)

                                                                                             CGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGGTAAATGAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTT  >  SRR4302533.1699595/1‑94 (MQ=60)

                                                                                             CGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTT  <  SRR4302533.2208184/94‑1 (MQ=60)

CCGCATCGGGCGACGCAGTTTCTATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTT  >  NC_000913/1461781‑1461967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`