Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,476,839 T→C V197A (GTC→GCC)  b1409 → putative phosphatidate cytidiltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,476,8390TC99.5% 577.3 / NA 182V197A (GTC→GCC) b1409putative phosphatidate cytidiltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (107/74);  minor base A (1/0);  total (108/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

GTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGTCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTAC  >  NC_000913/1476750‑1476928
                                                                                         |                                                                                         
gTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGcccc                                                                                        >  1:146166/1‑93 (MQ=255)
gTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGcccc                                                                                        >  1:71906/1‑93 (MQ=255)
 ttACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCt                                                                                       >  1:2459627/1‑93 (MQ=255)
  tgcTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTa                                                                                      >  2:4267095/3‑93 (MQ=255)
  tACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTa                                                                                      >  1:4395571/1‑93 (MQ=255)
  tACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTa                                                                                      >  2:4821803/1‑93 (MQ=255)
  tACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGACCCTa                                                                                      >  2:49248/1‑93 (MQ=255)
  tACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCATGCGGCAGAAGAAAAGCGGCCCCTa                                                                                      >  1:6085915/1‑93 (MQ=255)
   aCTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTaa                                                                                     >  1:4008294/1‑93 (MQ=255)
    cTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTaaa                                                                                    >  1:6255384/1‑93 (MQ=255)
    cTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTaaa                                                                                    <  2:5122968/93‑1 (MQ=255)
     tGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAg                                                                                   >  1:4594881/1‑93 (MQ=255)
      ggTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGATGAAAAGTGGCCCCTAAAGt                                                                                  <  2:6129989/93‑1 (MQ=255)
      ggTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGt                                                                                  >  2:5884061/1‑93 (MQ=255)
      ggTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGt                                                                                  <  2:4942801/93‑1 (MQ=255)
      ggTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGt                                                                                  >  2:3676255/1‑93 (MQ=255)
       gtgtTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTc                                                                                 <  2:3184390/93‑1 (MQ=255)
       gtgtTATTTCTTGTGGCATGAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTc                                                                                 <  2:567457/93‑1 (MQ=255)
        tgtTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCa                                                                                >  2:2998624/1‑93 (MQ=255)
          ttATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGc                                                                              >  1:1209939/1‑93 (MQ=255)
           tATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGGAAAGTGGCCCCTAAAGTCAGcc                                                                             <  1:4991123/93‑1 (MQ=255)
           tATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGcc                                                                             >  1:1424013/1‑93 (MQ=255)
            aTTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGccc                                                                            <  2:281225/93‑1 (MQ=255)
             tttCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCg                                                                           <  2:4165108/93‑1 (MQ=255)
             tttCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCg                                                                           <  2:1755480/93‑1 (MQ=255)
             tttCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTAAGCCCg                                                                           >  2:3353316/1‑93 (MQ=255)
              ttCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCgg                                                                          <  1:4643277/93‑1 (MQ=255)
               tCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCggg                                                                         >  1:5545361/1‑93 (MQ=255)
               tCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCggg                                                                         <  2:3505950/93‑1 (MQ=255)
               tCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCggg                                                                         >  1:1713668/1‑93 (MQ=255)
                cTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCTTGCGGCAGAAGAAAAGTGGCCCCTAAAATCAGCCCGGGa                                                                        <  2:3000371/93‑1 (MQ=255)
                   ttGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGaaaa                                                                     >  1:2961703/1‑93 (MQ=255)
                   ttGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGaaaa                                                                     <  2:2418214/93‑1 (MQ=255)
                    tGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGaaaaa                                                                    >  1:2880446/1‑93 (MQ=255)
                     ggCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAAc                                                                   >  1:3755176/1‑93 (MQ=255)
                     ggCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAAc                                                                   >  2:2370648/1‑93 (MQ=255)
                      gCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACa                                                                  <  1:835221/93‑1 (MQ=255)
                      gCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACa                                                                  >  2:4320169/1‑93 (MQ=255)
                      gCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACa                                                                  <  2:6252786/93‑1 (MQ=255)
                        aTTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAtt                                                                >  2:539505/1‑93 (MQ=255)
                        aTTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAtt                                                                >  1:2453741/1‑93 (MQ=255)
                        aTTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAtt                                                                >  1:2382072/1‑93 (MQ=255)
                        aTTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAtt                                                                <  1:1947702/93‑1 (MQ=255)
                         ttAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTg                                                               <  1:1898129/93‑1 (MQ=255)
                           aaCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGa                                                             >  2:5295126/1‑93 (MQ=255)
                            aCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGaa                                                            >  2:3313184/1‑93 (MQ=255)
                            aCCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGaa                                                            <  2:802651/93‑1 (MQ=255)
                             ccGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAg                                                           >  1:1577535/1‑93 (MQ=255)
                              cGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAgg                                                          >  1:4279104/1‑93 (MQ=255)
                              cGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAgg                                                          >  1:4442526/1‑93 (MQ=255)
                              cGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAgg                                                          >  2:2197046/1‑93 (MQ=255)
                               gAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGt                                                         >  1:2617860/1‑93 (MQ=255)
                               gAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGt                                                         >  2:5037086/1‑93 (MQ=255)
                               gAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGt                                                         >  1:3671110/1‑93 (MQ=255)
                                aGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTc                                                        <  2:1934529/93‑1 (MQ=255)
                                 gTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCt                                                       <  2:3734432/93‑1 (MQ=255)
                                   cAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGa                                                     <  2:4293911/93‑1 (MQ=255)
                                   cAAACCATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGa                                                     <  2:2542255/93‑1 (MQ=255)
                                    aaaCGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGAt                                                    >  1:2791288/1‑93 (MQ=255)
                                    aaaCGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGAt                                                    <  1:5155188/93‑1 (MQ=255)
                                     aaCGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATg                                                   >  2:5494830/1‑93 (MQ=255)
                                     aaCGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATg                                                   >  1:93437/1‑93 (MQ=255)
                                      aCGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATgg                                                  >  1:4569048/1‑93 (MQ=255)
                                        gATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggc                                                <  1:3937906/93‑1 (MQ=255)
                                          tatTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcgg                                              >  2:421352/1‑93 (MQ=255)
                                          tatTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcgg                                              >  2:4855028/1‑93 (MQ=255)
                                           atTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcggc                                             <  2:5738447/93‑1 (MQ=255)
                                           atTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcggc                                             >  1:200343/1‑93 (MQ=255)
                                           atTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGggcggc                                             >  2:6222799/1‑93 (MQ=255)
                                               cacaGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtca                                         >  1:110111/1‑93 (MQ=255)
                                                acaGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcat                                        >  1:1754529/1‑93 (MQ=255)
                                                acaGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcat                                        >  2:2396491/1‑93 (MQ=255)
                                                acaGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcat                                        >  1:3726768/1‑93 (MQ=255)
                                                 caGTATTTATGGGGAATATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcatc                                       <  2:3419649/93‑1 (MQ=255)
                                                 caGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGtcatc                                       <  2:2015040/93‑1 (MQ=255)
                                                    tATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCAcc                                    >  1:4891432/1‑93 (MQ=255)
                                                     aTTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAATTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACca                                   >  2:56309/1‑93 (MQ=255)
                                                                 aagtcTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGTGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGt                       <  2:4895445/89‑1 (MQ=255)
                                                                 aaTCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGt                       <  1:4750143/93‑1 (MQ=255)
                                                                  aTCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTc                      <  2:3577941/93‑1 (MQ=255)
                                                                  aTCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTc                      <  1:3275875/93‑1 (MQ=255)
                                                                   tCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCa                     <  1:510149/93‑1 (MQ=255)
                                                                   tCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCa                     >  1:5251067/1‑93 (MQ=255)
                                                                    ccTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCAc                    >  2:4534491/1‑93 (MQ=255)
                                                                     cTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACt                   >  1:1873441/1‑93 (MQ=255)
                                                                     cTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACt                   <  1:4074949/93‑1 (MQ=255)
                                                                         ggCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGAtt               <  2:5844524/93‑1 (MQ=255)
                                                                         ggCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGAtt               <  2:4022952/93‑1 (MQ=255)
                                                                         ggCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGAtt               <  1:479094/93‑1 (MQ=255)
                                                                         ggCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGAtt               >  2:1157145/1‑93 (MQ=255)
                                                                          gCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTa              >  1:2830569/1‑93 (MQ=255)
                                                                           cAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTAt             <  2:1641905/93‑1 (MQ=255)
                                                                            agaagaAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATc            <  1:4074960/93‑1 (MQ=255)
                                                                            agaagaAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATc            <  1:5881473/93‑1 (MQ=255)
                                                                                gaaAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGAc        >  2:69852/1‑93 (MQ=255)
                                                                                   aaGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGt     <  2:2378340/93‑1 (MQ=255)
                                                                                    aGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGtt    >  1:6165494/1‑93 (MQ=255)
                                                                                    aGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGtt    >  2:1131631/1‑93 (MQ=255)
                                                                                      tGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTAc  <  2:397961/93‑1 (MQ=255)
                                                                                      tGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTAc  <  2:1646321/93‑1 (MQ=255)
                                                                                         |                                                                                         
GTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGTCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTAC  >  NC_000913/1476750‑1476928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCGTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGTCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGAC  >  NC_000913/1476746‑1476932
                                                                                             |                                                                                             
GTGCGTTAGTGGTGTTATTTCTTTTGGCAGTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGC                                                                                               <  SRR4302533.5494667/94‑1 (MQ=60)
GGGCGTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGC                                                                                               <  SRR4302533.3674798/94‑1 (MQ=60)
GGGCGTTACTGGTGTGAGTTCTTTTGGCATTACCGGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGC                                                                                               <  SRR4302533.3308976/94‑1 (MQ=60)
 GGCGTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCC                                                                                              <  SRR4302533.4557311/94‑1 (MQ=60)
   CGTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCC                                                                                            >  SRR4302533.147100/1‑94 (MQ=60)
   CGTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCC                                                                                            >  SRR4302533.72367/1‑94 (MQ=60)
    GTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCT                                                                                           >  SRR4302533.2474805/1‑94 (MQ=60)
     TTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTA                                                                                          >  SRR4302533.4422109/1‑94 (MQ=60)
     TTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCATGCGGCAGAAGAAAAGCGGCCCCTA                                                                                          >  SRR4302533.6122708/1‑94 (MQ=60)
      TACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAA                                                                                         >  SRR4302533.4032700/1‑94 (MQ=60)
       ACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAA                                                                                        >  SRR4302533.2439388/1‑94 (MQ=60)
       ACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAA                                                                                        >  SRR4302533.6292792/1‑94 (MQ=60)
       ACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAA                                                                                        >  SRR4302533.4512278/1‑94 (MQ=60)
       ACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAA                                                                                        >  SRR4302533.4430869/1‑94 (MQ=60)
        CTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAG                                                                                       >  SRR4302533.1555629/1‑94 (MQ=60)
        CTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAG                                                                                       >  SRR4302533.4622715/1‑94 (MQ=60)
         TGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGT                                                                                      >  SRR4302533.5513986/1‑94 (MQ=60)
         TGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGT                                                                                      >  SRR4302533.4604301/1‑94 (MQ=60)
         TGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGT                                                                                      <  SRR4302533.4659327/94‑1 (MQ=60)
            TGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAG                                                                                   >  SRR4302533.3032681/1‑94 (MQ=60)
             GTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGC                                                                                  >  SRR4302533.1217204/1‑94 (MQ=60)
             GTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGC                                                                                  <  SRR4302533.488759/94‑1 (MQ=60)
              TTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCC                                                                                 >  SRR4302533.6169539/1‑94 (MQ=60)
              TTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCC                                                                                 >  SRR4302533.854602/1‑94 (MQ=60)
              TTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCC                                                                                 >  SRR4302533.1432625/1‑94 (MQ=60)
               TATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGGAAAGTGGCCCCTAAAGTCAGCCC                                                                                <  SRR4302533.5021383/94‑1 (MQ=60)
                  TTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGG                                                                             <  SRR4302533.4671409/94‑1 (MQ=60)
                  TTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGG                                                                             >  SRR4302533.5578856/1‑94 (MQ=60)
                  TTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGG                                                                             >  SRR4302533.1724055/1‑94 (MQ=60)
                    CTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAA                                                                           >  SRR4302533.4242140/1‑94 (MQ=60)
                      TTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAA                                                                         >  SRR4302533.2979807/1‑94 (MQ=60)
                       TTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAA                                                                        >  SRR4302533.2898167/1‑94 (MQ=60)
                        TGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAAC                                                                       >  SRR4302533.3778191/1‑94 (MQ=60)
                          GCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACAT                                                                     <  SRR4302533.840116/94‑1 (MQ=60)
                           CATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATT                                                                    >  SRR4302533.2396803/1‑94 (MQ=60)
                           CATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATT                                                                    >  SRR4302533.2468882/1‑94 (MQ=60)
                            ATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTG                                                                   <  SRR4302533.1959575/94‑1 (MQ=60)
                            ATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTG                                                                   >  SRR4302533.3881172/1‑94 (MQ=60)
                             TTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGG                                                                  <  SRR4302533.1909718/94‑1 (MQ=60)
                             TTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGG                                                                  >  SRR4302533.479398/1‑94 (MQ=60)
                                ACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAG                                                               >  SRR4302533.1587034/1‑94 (MQ=60)
                                 CCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGG                                                              >  SRR4302533.4469387/1‑94 (MQ=60)
                                 CCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGG                                                              >  SRR4302533.4304836/1‑94 (MQ=60)
                                 CCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGG                                                              <  SRR4302533.3988498/94‑1 (MQ=60)
                                  CGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGT                                                             >  SRR4302533.2633928/1‑94 (MQ=60)
                                  CGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGT                                                             >  SRR4302533.3693673/1‑94 (MQ=60)
                                       CAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGAT                                                        >  SRR4302533.2808457/1‑94 (MQ=60)
                                        AAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATG                                                       <  SRR4302533.4311115/94‑1 (MQ=60)
                                        AAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATG                                                       <  SRR4302533.5186319/94‑1 (MQ=60)
                                        AAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATG                                                       >  SRR4302533.94026/1‑94 (MQ=60)
                                        AAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATG                                                       <  SRR4302533.1959660/94‑1 (MQ=60)
                                         AACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGG                                                      >  SRR4302533.4596739/1‑94 (MQ=60)
                                           CGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGC                                                    <  SRR4302533.2186222/94‑1 (MQ=60)
                                            GATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCG                                                   <  SRR4302533.6258365/94‑1 (MQ=60)
                                            GATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCG                                                   >  SRR4302533.5030196/1‑94 (MQ=60)
                                            GATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCG                                                   <  SRR4302533.3961942/94‑1 (MQ=60)
                                              TATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGC                                                 >  SRR4302533.201613/1‑94 (MQ=60)
                                                TTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGT                                               <  SRR4302533.501952/94‑1 (MQ=60)
                                                  GCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCA                                             >  SRR4302533.110809/1‑94 (MQ=60)
                                                   CACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCAT                                            >  SRR4302533.2852652/1‑94 (MQ=60)
                                                   CACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCAT                                            <  SRR4302533.1456048/94‑1 (MQ=60)
                                                   CACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCAT                                            >  SRR4302533.469441/1‑94 (MQ=60)
                                                   CACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCAT                                            >  SRR4302533.1765213/1‑94 (MQ=60)
                                                   CACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCAT                                            >  SRR4302533.3749629/1‑94 (MQ=60)
                                                      AGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCAC                                         >  SRR4302533.3767726/1‑94 (MQ=60)
                                                       GTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACC                                        >  SRR4302533.4921068/1‑94 (MQ=60)
                                                          TTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATC                                     >  SRR4302533.694710/1‑94 (MQ=60)
                                                            TATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGCCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCAT                                   <  SRR4302533.3619793/94‑1 (MQ=60)
                                                               GGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGAT                                <  SRR4302533.1290410/94‑1 (MQ=60)
                                                               GGGGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGAT                                >  SRR4302533.1748613/1‑94 (MQ=60)
                                                                 GGAAAATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTG                              <  SRR4302533.2542069/94‑1 (MQ=60)
                                                                     AATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTC                          <  SRR4302533.4778944/94‑1 (MQ=60)
                                                                      ATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCA                         <  SRR4302533.3296101/94‑1 (MQ=60)
                                                                      ATCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCA                         >  SRR4302533.5282620/1‑94 (MQ=60)
                                                                       TCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCAC                        >  SRR4302533.4054668/1‑94 (MQ=60)
                                                                       TCCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCAC                        <  SRR4302533.513180/94‑1 (MQ=60)
                                                                        CCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACT                       >  SRR4302533.1884859/1‑94 (MQ=60)
                                                                        CCTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCCGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACT                       <  SRR4302533.5262318/94‑1 (MQ=60)
                                                                         CTGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTG                      <  SRR4302533.4099756/94‑1 (MQ=60)
                                                                          TGCGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCAACATCATGATTGCGTCACTGA                     >  SRR4302533.6065768/1‑94 (MQ=60)
                                                                            CGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATT                   >  SRR4302533.2779195/1‑94 (MQ=60)
                                                                            CGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATT                   <  SRR4302533.5585016/94‑1 (MQ=60)
                                                                            CGGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTCATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATT                   >  SRR4302533.3716678/1‑94 (MQ=60)
                                                                             GGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTA                  >  SRR4302533.2847990/1‑94 (MQ=60)
                                                                             GGCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTA                  <  SRR4302533.481957/94‑1 (MQ=60)
                                                                              GCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTAT                 <  SRR4302533.1626485/94‑1 (MQ=60)
                                                                              GCAGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTAT                 >  SRR4302533.1895357/1‑94 (MQ=60)
                                                                                AGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCG               <  SRR4302533.4099768/94‑1 (MQ=60)
                                                                                AGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCG               >  SRR4302533.1772349/1‑94 (MQ=60)
                                                                                AGAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCG               <  SRR4302533.5917016/94‑1 (MQ=60)
                                                                                 GAAGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGG              >  SRR4302533.2841099/1‑94 (MQ=60)
                                                                                   AGAAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGAC            >  SRR4302533.76741/1‑94 (MQ=60)
                                                                                     AAAAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCG          <  SRR4302533.1944296/94‑1 (MQ=60)
                                                                                       AAGTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTT        >  SRR4302533.6202555/1‑94 (MQ=60)
                                                                                         GTGGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTAC      >  SRR4302533.293879/1‑94 (MQ=60)
                                                                                           GGCCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTG    >  SRR4302533.400733/1‑94 (MQ=60)
                                                                                             CCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGAC  <  SRR4302533.548443/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGGCGTTACTGGTGTTATTTCTTTTGGCATTAACCGAGTCAAACGATATTGCACAGTATTTATGGGGAAAATCCTGCGGCAGAAGAAAAGTGGTCCCTAAAGTCAGCCCGGGAAAAACATTGGAAGGTCTGATGGGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGAC  >  NC_000913/1476746‑1476932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑