Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,531,834 A→G R30R (CGT→CGC)  b1462 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,531,8340AG100.0% 608.1 / NA 191R30R (CGT→CGC) b1462hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (79/112);  total (79/112)

Only 100 of 197 total aligned reads displayed.

CGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAA  >  NC_000913/1531744‑1531923
                                                                                          |                                                                                         
 ggtaTTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCgg                                                                                        >  2:1693677/4‑93 (MQ=255)
 ggCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCgg                                                                                        <  2:5577118/93‑1 (MQ=255)
  gCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAggcggc                                                                                       >  1:1162615/1‑93 (MQ=255)
  gCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAggcggc                                                                                       <  1:85492/93‑1 (MQ=255)
    aTTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTg                                                                                     <  1:2559671/93‑1 (MQ=255)
     ttGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTgg                                                                                    >  1:881890/1‑93 (MQ=255)
     ttGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTgg                                                                                    >  2:4715641/1‑93 (MQ=255)
       gACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCa                                                                                  >  2:1354993/1‑93 (MQ=255)
       gACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCa                                                                                  >  2:158323/1‑93 (MQ=255)
        aCCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCAt                                                                                 <  1:4606583/93‑1 (MQ=255)
        aCCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCAt                                                                                 >  1:1685295/1‑93 (MQ=255)
            ggCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGgtg                                                                             <  2:3048422/93‑1 (MQ=255)
               tGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTaa                                                                          <  2:2564045/93‑1 (MQ=255)
               tGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTaa                                                                          >  1:4050013/1‑93 (MQ=255)
                gCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAAt                                                                         >  1:2790527/1‑93 (MQ=255)
                gCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAAt                                                                         <  2:2060207/93‑1 (MQ=255)
                   gCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCt                                                                      >  1:5538463/1‑93 (MQ=255)
                       tAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTTTAATTCTATgg                                                                  <  2:2692965/93‑1 (MQ=255)
                       tAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATgg                                                                  >  1:2417949/1‑93 (MQ=255)
                       tAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATgg                                                                  >  1:451438/1‑93 (MQ=255)
                       tAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATgg                                                                  >  1:1083669/1‑93 (MQ=255)
                       tAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGGGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGGGTAATTCTATgg                                                                  >  1:2985801/1‑93 (MQ=255)
                       tAATGCTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGCCCGTTGGACCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATgg                                                                  <  1:6131067/93‑1 (MQ=255)
                        aaTGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATggg                                                                 >  1:1215350/1‑93 (MQ=255)
                          tGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGa                                                               >  1:2760244/1‑93 (MQ=255)
                           gTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGAt                                                              <  2:3702121/93‑1 (MQ=255)
                             tACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGa                                                            <  1:4837303/93‑1 (MQ=255)
                             tACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGa                                                            <  1:4537206/93‑1 (MQ=255)
                             tACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGa                                                            >  2:3634706/1‑93 (MQ=255)
                             tACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGa                                                            <  2:2270687/93‑1 (MQ=255)
                              aCGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGaa                                                           >  1:434295/1‑93 (MQ=255)
                              aCGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGaa                                                           <  2:4080356/93‑1 (MQ=255)
                                gccgcTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATc                                                         <  2:5142501/93‑1 (MQ=255)
                                gccgcTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATc                                                         >  2:487841/1‑93 (MQ=255)
                                  cgcTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCgg                                                       >  2:4896256/1‑93 (MQ=255)
                                   gcTGGGATTGCTCATCGAAGCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGc                                                      <  2:1801132/93‑1 (MQ=255)
                                   gcTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGc                                                      >  2:1218981/1‑93 (MQ=255)
                                      gggATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCa                                                   >  2:1275550/1‑93 (MQ=255)
                                      gggATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCa                                                   <  2:3798763/93‑1 (MQ=255)
                                        gATTGCTCATCGAAACTGGCGATCAAGACCGTTGGGCCAAGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCCTg                                                 >  2:198257/1‑93 (MQ=255)
                                         aTTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATgg                                                <  1:3552837/93‑1 (MQ=255)
                                          ttGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGt                                               <  1:352758/93‑1 (MQ=255)
                                           tGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTa                                              >  1:5702330/1‑93 (MQ=255)
                                           tGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTa                                              >  1:767604/1‑93 (MQ=255)
                                             cTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATc                                            >  1:3028561/1‑93 (MQ=255)
                                              tCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCg                                           >  1:2834717/1‑93 (MQ=255)
                                                 tCGAAACTGGTGATCAAGTCTGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCt                                        <  1:2101460/93‑1 (MQ=255)
                                                  cGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTc                                       <  1:1868081/93‑1 (MQ=255)
                                                  cGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTc                                       <  2:5357295/93‑1 (MQ=255)
                                                   gAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcg                                      >  2:2650311/1‑93 (MQ=255)
                                                   gAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcg                                      <  2:1916763/93‑1 (MQ=255)
                                                    aaaCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcgc                                     >  1:4927119/1‑93 (MQ=255)
                                                    aaaCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcgc                                     <  2:3543920/93‑1 (MQ=255)
                                                    aaaCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcgc                                     >  2:5595945/1‑93 (MQ=255)
                                                    aaaCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcgc                                     <  1:3228925/93‑1 (MQ=255)
                                                     aaCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTcgcg                                    >  1:679342/1‑93 (MQ=255)
                                                      aCTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCga                                   <  2:5305093/93‑1 (MQ=255)
                                                       cTGTTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCgaa                                  <  2:5998949/93‑1 (MQ=255)
                                                       cTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCgaa                                  <  1:5766041/93‑1 (MQ=255)
                                                         ggTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCgaaga                                <  1:2315011/93‑1 (MQ=255)
                                                         ggTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCgaaga                                <  2:3535792/93‑1 (MQ=255)
                                                          gTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCgaagaa                               <  2:1594022/93‑1 (MQ=255)
                                                             aTCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGt                            <  2:4532333/93‑1 (MQ=255)
                                                             aTCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGt                            <  2:751863/93‑1 (MQ=255)
                                                             aTCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGt                            <  1:4764808/93‑1 (MQ=255)
                                                              tCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGtt                           >  1:3858004/1‑93 (MQ=255)
                                                              tCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGtt                           <  2:1446232/93‑1 (MQ=255)
                                                               cAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttt                          <  1:1309682/93‑1 (MQ=255)
                                                               cAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttt                          <  2:5982044/93‑1 (MQ=255)
                                                                aaGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGtttt                         <  2:252354/93‑1 (MQ=255)
                                                                 agtccGTTGGGCAATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttttt                        <  1:5292578/90‑1 (MQ=255)
                                                                 aGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttttt                        >  1:5638119/1‑93 (MQ=255)
                                                                 aGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttttt                        <  2:4401639/93‑1 (MQ=255)
                                                                 aGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttttt                        <  1:1577183/93‑1 (MQ=255)
                                                                   aCCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGttttttt                      >  1:4095556/1‑93 (MQ=255)
                                                                     cGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTaa                    <  2:3207407/93‑1 (MQ=255)
                                                                     cGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTaa                    >  2:5893454/1‑93 (MQ=255)
                                                                      gTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAg                   >  1:1602860/1‑93 (MQ=255)
                                                                      gTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAg                   <  2:461365/93‑1 (MQ=255)
                                                                      gTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAg                   <  1:3463298/93‑1 (MQ=255)
                                                                      gTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAg                   >  2:4090978/1‑93 (MQ=255)
                                                                      gTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAg                   >  2:3122805/1‑93 (MQ=255)
                                                                      gTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAg                   <  1:3675655/93‑1 (MQ=255)
                                                                       ttGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGc                  <  2:4171547/93‑1 (MQ=255)
                                                                        tGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCg                 <  2:3855297/93‑1 (MQ=255)
                                                                        tGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCg                 <  1:4273070/93‑1 (MQ=255)
                                                                          ggCCATGATTAAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTa               <  1:2149487/93‑1 (MQ=255)
                                                                             cATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTc            >  2:4237725/1‑93 (MQ=255)
                                                                             cATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGGTTTTTTAAGCGTAGTc            >  1:2181377/1‑93 (MQ=255)
                                                                              aTGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTcc           >  1:5096746/1‑93 (MQ=255)
                                                                               tGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCg          <  1:1318620/93‑1 (MQ=255)
                                                                               tGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCg          >  2:4813713/1‑93 (MQ=255)
                                                                                gATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATTGGATCGCCTCGCCAAGAAGGTTTTTTTAAACGTAGTCCGt         >  2:334437/1‑93 (MQ=255)
                                                                                gATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGt         <  2:5454136/93‑1 (MQ=255)
                                                                                  tttAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTaa       <  2:5398270/93‑1 (MQ=255)
                                                                                  tttAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTaa       <  2:4662013/93‑1 (MQ=255)
                                                                                  tttAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTaa       <  1:2926560/93‑1 (MQ=255)
                                                                                   ttAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAAc      >  1:722748/1‑93 (MQ=255)
                                                                                       cAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCaa  <  1:1398200/93‑1 (MQ=255)
                                                                                       cAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCaa  >  2:862841/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAA  >  NC_000913/1531744‑1531923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAATAAG  >  NC_000913/1531741‑1531927
                                                                                             |                                                                                             
CACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGG                                                                                               <  SRR4302533.4580627/94‑1 (MQ=60)
  CCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCG                                                                                             >  SRR4302533.2288853/1‑94 (MQ=60)
    GGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGC                                                                                           >  SRR4302533.1169590/1‑94 (MQ=60)
     GCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCT                                                                                          <  SRR4302533.86041/94‑1 (MQ=60)
       ATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGG                                                                                        >  SRR4302533.887070/1‑94 (MQ=60)
       ATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGG                                                                                        <  SRR4302533.2912462/94‑1 (MQ=60)
       ATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGG                                                                                        <  SRR4302533.2575438/94‑1 (MQ=60)
       ATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGG                                                                                        >  SRR4302533.2191341/1‑94 (MQ=60)
         TGACCAGGCTGCAGCCATCATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCA                                                                                      >  SRR4302533.2658425/1‑94 (MQ=60)
          TACCAGGCTGCAGCCATAATGTTACGCAGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCAT                                                                                     <  SRR4302533.875754/94‑1 (MQ=60)
          GACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCAT                                                                                     >  SRR4302533.1695504/1‑94 (MQ=60)
           ACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATG                                                                                    <  SRR4302533.4634486/94‑1 (MQ=60)
            CCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGG                                                                                   <  SRR4302533.4853269/94‑1 (MQ=60)
            CCAGGCGGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGG                                                                                   <  SRR4302533.1862819/94‑1 (MQ=60)
             CAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGT                                                                                  >  SRR4302533.3659798/1‑94 (MQ=60)
              AGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTG                                                                                 >  SRR4302533.690542/1‑94 (MQ=60)
                GCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTA                                                                               >  SRR4302533.4119060/1‑94 (MQ=60)
                GCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTA                                                                               <  SRR4302533.3515369/94‑1 (MQ=60)
                 CTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAA                                                                              >  SRR4302533.4074683/1‑94 (MQ=60)
                 CTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGGGTAA                                                                              >  SRR4302533.1226623/1‑94 (MQ=60)
                  TGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAAT                                                                             >  SRR4302533.2807690/1‑94 (MQ=60)
                   TCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATT                                                                            <  SRR4302533.4202599/94‑1 (MQ=60)
                     AGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCT                                                                          >  SRR4302533.5571909/1‑94 (MQ=60)
                      GCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTA                                                                         >  SRR4302533.2836787/1‑94 (MQ=60)
                       CCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTAT                                                                        <  SRR4302533.2790186/94‑1 (MQ=60)
                        CATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATG                                                                       <  SRR4302533.6295150/94‑1 (MQ=60)
                        CATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATG                                                                       <  SRR4302533.3566752/94‑1 (MQ=60)
                         ATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGG                                                                      >  SRR4302533.1090060/1‑94 (MQ=60)
                         ATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGG                                                                      >  SRR4302533.2432882/1‑94 (MQ=60)
                         ATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGG                                                                      >  SRR4302533.454139/1‑94 (MQ=60)
                         ATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGG                                                                      <  SRR4302533.1656037/94‑1 (MQ=60)
                         ATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGGGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGGGTAATTCTATGG                                                                      >  SRR4302533.3004070/1‑94 (MQ=60)
                          TAATGTTACGCCGCTGGTATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGG                                                                     <  SRR4302533.505447/94‑1 (MQ=60)
                          TAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGG                                                                     >  SRR4302533.1222648/1‑94 (MQ=60)
                          TAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGG                                                                     <  SRR4302533.2475039/94‑1 (MQ=60)
                          TAATGCTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGCCCGTTGGACCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGG                                                                     <  SRR4302533.6168010/94‑1 (MQ=60)
                            ATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGA                                                                   >  SRR4302533.2777160/1‑94 (MQ=60)
                             TGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGAT                                                                  <  SRR4302533.1217506/94‑1 (MQ=60)
                             AGTTACGCCGCTGGGATTGCTCATCAAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGAT                                                                  <  SRR4302533.4912952/94‑1 (MQ=60)
                               TTCCGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGCCCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGA                                                                <  SRR4302533.307472/94‑1 (MQ=60)
                                TACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAA                                                               <  SRR4302533.4866620/94‑1 (MQ=60)
                                TACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAA                                                               <  SRR4302533.4564677/94‑1 (MQ=60)
                                TACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAA                                                               >  SRR4302533.436884/1‑94 (MQ=60)
                                 ACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAAT                                                              <  SRR4302533.5258182/94‑1 (MQ=60)
                                  CGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATC                                                             <  SRR4302533.835314/94‑1 (MQ=60)
                                          GGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATG                                                     >  SRR4302533.4509559/1‑94 (MQ=60)
                                          GCATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATG                                                     <  SRR4302533.5582007/94‑1 (MQ=60)
                                           GATTGCTCATCGAAACTGGTGATCAAGGCCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGG                                                    <  SRR4302533.4692395/94‑1 (MQ=60)
                                            ATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGT                                                   <  SRR4302533.2388183/94‑1 (MQ=60)
                                            ATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGT                                                   >  SRR4302533.750916/1‑94 (MQ=60)
                                            ATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGT                                                   <  SRR4302533.3574720/94‑1 (MQ=60)
                                             TTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTA                                                  >  SRR4302533.772132/1‑94 (MQ=60)
                                             TTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTA                                                  <  SRR4302533.354909/94‑1 (MQ=60)
                                             TTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTA                                                  >  SRR4302533.5736765/1‑94 (MQ=60)
                                               GCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATC                                                >  SRR4302533.3047094/1‑94 (MQ=60)
                                                CTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCG                                               >  SRR4302533.2852164/1‑94 (MQ=60)
                                                CTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCG                                               <  SRR4302533.159547/94‑1 (MQ=60)
                                                 TCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGC                                              <  SRR4302533.5556803/94‑1 (MQ=60)
                                                    TCGAAACTGGTGATCAAGTCTGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTC                                           <  SRR4302533.2114420/94‑1 (MQ=60)
                                                     CGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCG                                          <  SRR4302533.1879470/94‑1 (MQ=60)
                                                      GAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGC                                         >  SRR4302533.4956959/1‑94 (MQ=60)
                                                       AAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCG                                        >  SRR4302533.683356/1‑94 (MQ=60)
                                                       AAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCG                                        <  SRR4302533.3248832/94‑1 (MQ=60)
                                                         ACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAA                                      >  SRR4302533.3780182/1‑94 (MQ=60)
                                                         ACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAA                                      <  SRR4302533.3321666/94‑1 (MQ=60)
                                                          CTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGCAG                                     >  SRR4302533.4546884/1‑94 (MQ=60)
                                                          CTGGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAG                                     <  SRR4302533.5800854/94‑1 (MQ=60)
                                                            GGTGATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAA                                   <  SRR4302533.2329350/94‑1 (MQ=60)
                                                                ATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTT                               <  SRR4302533.4793705/94‑1 (MQ=60)
                                                                ATCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTT                               >  SRR4302533.3881561/1‑94 (MQ=60)
                                                                 TCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTT                              <  SRR4302533.2009295/94‑1 (MQ=60)
                                                                 TCAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTT                              >  SRR4302533.1325838/1‑94 (MQ=60)
                                                                  CAAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTT                             <  SRR4302533.1317544/94‑1 (MQ=60)
                                                                   AAGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTT                            >  SRR4302533.5672208/1‑94 (MQ=60)
                                                                    AGTCCGTTGGGCAATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTT                           <  SRR4302533.5324260/94‑1 (MQ=60)
                                                                    AGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTT                           <  SRR4302533.1586680/94‑1 (MQ=60)
                                                                    AGACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTT                           >  SRR4302533.1356681/1‑94 (MQ=60)
                                                                     GACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTT                          >  SRR4302533.4120488/1‑94 (MQ=60)
                                                                     GACCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTT                          <  SRR4302533.6073371/94‑1 (MQ=60)
                                                                       CCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAA                        >  SRR4302533.4051482/1‑94 (MQ=60)
                                                                       CCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAA                        >  SRR4302533.3038431/1‑94 (MQ=60)
                                                                       CCGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGGTTTTTTAA                        >  SRR4302533.5699334/1‑94 (MQ=60)
                                                                        CGTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAG                       >  SRR4302533.1612566/1‑94 (MQ=60)
                                                                         GTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGC                      <  SRR4302533.3698243/94‑1 (MQ=60)
                                                                         GTTGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGC                      <  SRR4302533.3484677/94‑1 (MQ=60)
                                                                           TGGGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGT                    <  SRR4302533.4298783/94‑1 (MQ=60)
                                                                             GGCCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAG                  <  SRR4302533.1583071/94‑1 (MQ=60)
                                                                             GGCCATGATTAAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAG                  <  SRR4302533.2162723/94‑1 (MQ=60)
                                                                               CCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTC                <  SRR4302533.5775101/94‑1 (MQ=60)
                                                                               CCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTC                >  SRR4302533.2975413/1‑94 (MQ=60)
                                                                               CCATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGGTTTTTTAAGCGTAGTC                >  SRR4302533.2194826/1‑94 (MQ=60)
                                                                                CATGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCC               >  SRR4302533.5127540/1‑94 (MQ=60)
                                                                                  TGATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGT             <  SRR4302533.1326529/94‑1 (MQ=60)
                                                                                   GATTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTA            <  SRR4302533.4303562/94‑1 (MQ=60)
                                                                                     TTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAAC          <  SRR4302533.2944464/94‑1 (MQ=60)
                                                                                     TTTAACAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAAC          >  SRR4302533.727022/1‑94 (MQ=60)
                                                                                        AACAGGCGGCTGCGATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCA       <  SRR4302533.2120358/94‑1 (MQ=60)
                                                                                          CAGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAAT     <  SRR4302533.1406627/94‑1 (MQ=60)
                                                                                           AGGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAATA    <  SRR4302533.3330135/94‑1 (MQ=60)
                                                                                            GGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCCATAA   >  SRR4302533.5648215/1‑94 (MQ=60)
                                                                                            GGCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAATAA   <  SRR4302533.2993912/94‑1 (MQ=60)
                                                                                             GCGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAATAAG  <  SRR4302533.5219083/94‑1 (MQ=60)
                                                                                             |                                                                                             
CACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAACGCAATAAG  >  NC_000913/1531741‑1531927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑