Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,666,834	C→T	P261P (CCG→CCA)	ynfL ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,666,834	0	C	T	99.5%	760.8 / NA	203	P261P (CCG→CCA)	ynfL	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (99/103); minor base A (1/0); total (100/103)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

ATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGA  >  NC_000913/1666744‑1666922

aTGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTgg                                                                                        <  2:5362375/93‑1 (MQ=255)

 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       <  1:1190599/93‑1 (MQ=255)

 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       >  1:6288087/1‑93 (MQ=255)

 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       >  2:2238692/1‑93 (MQ=255)

 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       >  2:2533426/1‑93 (MQ=255)

  gAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCa                                                                                      >  2:3023477/1‑93 (MQ=255)

  gAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCa                                                                                      <  2:205237/93‑1 (MQ=255)

   aaTACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAc                                                                                     >  2:3116286/1‑93 (MQ=255)

     tACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAccc                                                                                   >  2:3877113/1‑93 (MQ=255)

     tACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAccc                                                                                   <  2:1470850/93‑1 (MQ=255)

       cGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAg                                                                                 <  1:1326678/93‑1 (MQ=255)

       cGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAg                                                                                 <  1:4537480/93‑1 (MQ=255)

         aaaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcg                                                                               >  2:825799/1‑93 (MQ=255)

          aaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcgc                                                                              <  1:457810/93‑1 (MQ=255)

          aaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcgc                                                                              >  2:1207401/1‑93 (MQ=255)

          aaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcgc                                                                              >  2:2553871/1‑93 (MQ=255)

            gTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCAt                                                                            <  1:4632949/93‑1 (MQ=255)

            gTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCAt                                                                            <  1:2208914/93‑1 (MQ=255)

             ttACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCAtt                                                                           <  1:5666579/93‑1 (MQ=255)

               aCGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTc                                                                         >  2:1750723/1‑93 (MQ=255)

               aCGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTc                                                                         <  1:1599849/93‑1 (MQ=255)

                  cgcAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGtt                                                                      >  2:2118469/1‑93 (MQ=255)

                  cgcAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGtt                                                                      <  1:1691091/93‑1 (MQ=255)

                   gcAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTg                                                                     >  2:3442759/1‑93 (MQ=255)

                    cAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGttca                                                                    <  1:6089715/93‑3 (MQ=255)

                    cAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGa                                                                    <  1:5732203/93‑1 (MQ=255)

                     aGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAg                                                                   >  1:4680047/1‑93 (MQ=255)

                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  1:1143306/1‑93 (MQ=255)

                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  <  2:4214287/93‑1 (MQ=255)

                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  2:2124846/1‑93 (MQ=255)

                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  2:2657526/1‑93 (MQ=255)

                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  2:2363886/1‑93 (MQ=255)

                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  <  1:3859310/93‑1 (MQ=255)

                        cGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTg                                                                <  2:8136/93‑1 (MQ=255)

                        cGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTg                                                                >  1:5307186/1‑93 (MQ=255)

                        cGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTg                                                                >  2:1518316/1‑93 (MQ=255)

                          gACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGaa                                                              <  2:6259889/93‑1 (MQ=255)

                           aCTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAac                                                             >  1:1950142/1‑93 (MQ=255)

                            cTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAaca                                                            <  1:4086590/93‑1 (MQ=255)

                            cTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATAGGCACCCAGCGCATTTCGTTGAGCTGAaca                                                            >  2:5445783/1‑93 (MQ=255)

                                gTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGtt                                                        <  1:5129770/93‑1 (MQ=255)

                                 ttCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGttt                                                       >  2:138192/1‑93 (MQ=255)

                                  tCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTa                                                      <  1:3964081/93‑1 (MQ=255)

                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     >  2:3298619/1‑93 (MQ=255)

                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     >  2:3486892/1‑93 (MQ=255)

                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     >  2:2725751/1‑93 (MQ=255)

                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     <  1:3100620/93‑1 (MQ=255)

                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     <  2:3930974/93‑1 (MQ=255)

                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     <  1:2567955/93‑1 (MQ=255)

                                    atgatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaaa                                                    <  1:3262919/93‑1 (MQ=255)

                                    atgatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaaa                                                    >  1:90682/1‑93 (MQ=255)

                                     tgatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAAt                                                   <  1:1767219/93‑1 (MQ=255)

                                      gatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATg                                                  >  1:498869/1‑93 (MQ=255)

                                      gatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATg                                                  <  2:1557322/93‑1 (MQ=255)

                                      gatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATg                                                  <  1:1614257/93‑1 (MQ=255)

                                      gatgTTTCGGCCAGAACAACCACATTTCAGAAACCGCATCCTCTTCAGCAACTGGCACCCAGCGCATTTCGTTGAGCTTAGCACGTTAAAATg                                                  >  2:3670485/1‑93 (MQ=255)

                                       atgTTTCGGCCAGACCAACCACATTTCAGAAGCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGa                                                 <  2:1857713/93‑1 (MQ=255)

                                       atgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGa                                                 >  1:5403300/1‑93 (MQ=255)

                                        tgTTTCGGCCGGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGAc                                                <  2:4826971/93‑1 (MQ=255)

                                        tgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGAc                                                <  2:3394717/93‑1 (MQ=255)

                                        tgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGAc                                                <  2:3510715/93‑1 (MQ=255)

                                         gTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACg                                               <  1:4709483/93‑1 (MQ=255)

                                          tttCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGc                                              <  2:5706545/93‑1 (MQ=255)

                                             cGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAgg                                           >  2:5953047/1‑93 (MQ=255)

                                                ccAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaa                                        >  2:4673083/1‑93 (MQ=255)

                                                 cAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaa                                       >  1:426536/1‑93 (MQ=255)

                                                 cAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaa                                       <  1:2321350/93‑1 (MQ=255)

                                                  aGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaaa                                      >  1:2949783/1‑93 (MQ=255)

                                                  aGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaaa                                      >  1:638809/1‑93 (MQ=255)

                                                     ccacccacATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTg                                   <  1:2795366/93‑1 (MQ=255)

                                                      caaccaCATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGa                                  >  2:3222332/1‑93 (MQ=255)

                                                       caccaCATTTCAGAAACCGCATCCTCCTCAACAATTGGCCCCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGaa                                 <  1:2645679/92‑1 (MQ=255)

                                                        accaCATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGaaa                                >  2:6017242/1‑93 (MQ=255)

                                                           acaTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAacac                             >  2:2030296/1‑93 (MQ=255)

                                                            caTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACAcc                            >  1:3346623/1‑93 (MQ=255)

                                                             aTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACAccc                           <  1:3170893/93‑1 (MQ=255)

                                                             aTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACAccc                           <  1:2995174/93‑1 (MQ=255)

                                                              tttCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCa                          <  1:5344882/93‑1 (MQ=255)

                                                              tttCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCa                          >  2:1968946/1‑93 (MQ=255)

                                                              tttCAGAAACCGCAGCCTTTTGAGCAATTGGCACCCAGGGCATTTCGTTGAGCCGAAAGCGTTTAAATGACGCAGGCAAAATTGAACCACCCa                          <  2:1280745/93‑1 (MQ=255)

                                                                tCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGa                        <  1:401816/93‑1 (MQ=255)

                                                                   gAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcg                     >  1:107248/1‑93 (MQ=255)

                                                                   gAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcg                     >  1:1552669/1‑93 (MQ=255)

                                                                    aaaCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcgg                    >  2:1647595/1‑93 (MQ=255)

                                                                     aaCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcggc                   <  2:5801428/93‑1 (MQ=255)

                                                                      aCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcggcg                  >  2:1771525/1‑93 (MQ=255)

                                                                        cGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGa                >  1:2280923/1‑93 (MQ=255)

                                                                          cATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGaaa              <  2:3746155/93‑1 (MQ=255)

                                                                           aTCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAc             <  2:1031259/93‑1 (MQ=255)

                                                                            tCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAcc            >  2:1109224/1‑93 (MQ=255)

                                                                            tCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAca            >  1:3637769/1‑92 (MQ=255)

                                                                             ccTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCa           >  2:5970610/1‑93 (MQ=255)

                                                                              ctctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAg          >  2:1803180/1‑93 (MQ=255)

                                                                               tctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGa         >  1:1392650/1‑93 (MQ=255)

                                                                               tctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGa         <  1:2124425/93‑1 (MQ=255)

                                                                                ctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGAc        <  2:3195510/93‑1 (MQ=255)

                                                                                   cAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCga     >  2:6283951/1‑93 (MQ=255)

                                                                                    aGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCgat    <  2:5116898/93‑1 (MQ=255)

                                                                                    aGCAATTGGCACCCAACGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCgat    >  2:2847516/1‑93 (MQ=255)

                                                                                     gCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCgatg   >  2:5505772/1‑93 (MQ=255)

ATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGA  >  NC_000913/1666744‑1666922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGT  >  NC_000913/1666741‑1666926

CAGATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATT                                                                                              >  SRR4302533.182426/1‑94 (MQ=60)

 AGATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTG                                                                                             >  SRR4302533.5993737/1‑94 (MQ=60)

 AGATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTG                                                                                             >  SRR4302533.5038173/1‑94 (MQ=60)

 AGATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTG                                                                                             >  SRR4302533.5443483/1‑94 (MQ=60)

  GATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGG                                                                                            <  SRR4302533.3217587/94‑1 (MQ=60)

  GATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGG                                                                                            <  SRR4302533.1883089/94‑1 (MQ=60)

   ATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGC                                                                                           >  SRR4302533.6325599/1‑94 (MQ=60)

   ATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGC                                                                                           >  SRR4302533.4677540/1‑94 (MQ=60)

    TGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCA                                                                                          <  SRR4302533.1574389/94‑1 (MQ=60)

    TGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCA                                                                                          <  SRR4302533.1197754/94‑1 (MQ=60)

     GAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAC                                                                                         >  SRR4302533.1850993/1‑94 (MQ=60)

     GAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAC                                                                                         <  SRR4302533.1276616/94‑1 (MQ=60)

     GAATACGAAAGTTACGCGCAGCCGGACTTTGTTCACGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAC                                                                                         <  SRR4302533.3286121/94‑1 (MQ=60)

         ACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAG                                                                                     <  SRR4302533.1479998/94‑1 (MQ=60)

         ACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAG                                                                                     <  SRR4302533.3644352/94‑1 (MQ=60)

         ACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAG                                                                                     <  SRR4302533.4591/94‑1 (MQ=60)

         ACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAG                                                                                     <  SRR4302533.5365623/94‑1 (MQ=60)

          CGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGC                                                                                    <  SRR4302533.4564952/94‑1 (MQ=60)

          CGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGC                                                                                    <  SRR4302533.1334637/94‑1 (MQ=60)

            AAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGC                                                                                  >  SRR4302533.3034935/1‑94 (MQ=60)

             AAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCA                                                                                 <  SRR4302533.460547/94‑1 (MQ=60)

               GTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATT                                                                               <  SRR4302533.4661025/94‑1 (MQ=60)

               GTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATT                                                                               <  SRR4302533.2222538/94‑1 (MQ=60)

                TTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTT                                                                              <  SRR4302533.5700810/94‑1 (MQ=60)

                  ACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCG                                                                            <  SRR4302533.1609533/94‑1 (MQ=60)

                   CGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGT                                                                           >  SRR4302533.5708897/1‑94 (MQ=60)

                    GCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTT                                                                          <  SRR4302533.1082487/94‑1 (MQ=60)

                     CGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTG                                                                         >  SRR4302533.5404297/1‑94 (MQ=60)

                     CGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTG                                                                         <  SRR4302533.1701335/94‑1 (MQ=60)

                       CAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAG                                                                       >  SRR4302533.2327762/1‑94 (MQ=60)

                       CAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAG                                                                       >  SRR4302533.4708409/1‑94 (MQ=60)

                       CAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAG                                                                       <  SRR4302533.5766808/94‑1 (MQ=60)

                       CAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTCAG                                                                       <  SRR4302533.6126524/94‑1 (MQ=60)

                        AGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGC                                                                      >  SRR4302533.1176181/1‑94 (MQ=60)

                        AGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGC                                                                      >  SRR4302533.1150125/1‑94 (MQ=60)

                         GCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCT                                                                     <  SRR4302533.3882872/94‑1 (MQ=60)

                          CCGGACTTTGTTCATGATGTTTCGGCCAGACTAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGATCTG                                                                    <  SRR4302533.1622040/94‑1 (MQ=60)

                          CCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTG                                                                    >  SRR4302533.722046/1‑94 (MQ=60)

                          CCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTG                                                                    >  SRR4302533.5338994/1‑94 (MQ=60)

                           CGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGA                                                                   <  SRR4302533.5331576/94‑1 (MQ=60)

                            GGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAA                                                                  >  SRR4302533.634778/1‑94 (MQ=60)

                             GACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTNANCNGNAC                                                                 >  SRR4302533.1894247/1‑94 (MQ=60)

                             GACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAAC                                                                 >  SRR4302533.1962028/1‑94 (MQ=60)

                               CTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACAC                                                               <  SRR4302533.6244717/94‑1 (MQ=60)

                               CTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACAC                                                               >  SRR4302533.5684395/1‑94 (MQ=60)

                               CTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACAC                                                               <  SRR4302533.4111469/94‑1 (MQ=60)

                                  TGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTT                                                            <  SRR4302533.3267057/94‑1 (MQ=60)

                                   GTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTT                                                           <  SRR4302533.5160760/94‑1 (MQ=60)

                                     TCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAA                                                         <  SRR4302533.3988229/94‑1 (MQ=60)

                                      CATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAA                                                        <  SRR4302533.3119680/94‑1 (MQ=60)

                                      CATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAA                                                        <  SRR4302533.2583758/94‑1 (MQ=60)

                                      CATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAA                                                        >  SRR4302533.91256/1‑94 (MQ=60)

                                       ATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAAT                                                       <  SRR4302533.3283048/94‑1 (MQ=60)

                                       ATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAAT                                                       >  SRR4302533.1690845/1‑94 (MQ=60)

                                       ATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAAT                                                       <  SRR4302533.4731819/94‑1 (MQ=60)

                                        TGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATG                                                      <  SRR4302533.1777979/94‑1 (MQ=60)

                                        TGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATG                                                      >  SRR4302533.2839135/1‑94 (MQ=60)

                                        TGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATG                                                      >  SRR4302533.501838/1‑94 (MQ=60)

                                         GATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGA                                                     >  SRR4302533.5435767/1‑94 (MQ=60)

                                         GATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGA                                                     <  SRR4302533.4324699/94‑1 (MQ=60)

                                         GATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGA                                                     <  SRR4302533.1624042/94‑1 (MQ=60)

                                           TGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACG                                                   <  SRR4302533.2560159/94‑1 (MQ=60)

                                            GTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGC                                                  <  SRR4302533.4738027/94‑1 (MQ=60)

                                             TTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCA                                                 >  SRR4302533.4649036/1‑94 (MQ=60)

                                              TTCAGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAG                                                <  SRR4302533.4964908/94‑1 (MQ=60)

                                                   CCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAA                                           >  SRR4302533.429081/1‑94 (MQ=60)

                                                    CAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAA                                          >  SRR4302533.1894522/1‑94 (MQ=60)

                                                    CAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAA                                          >  SRR4302533.2967822/1‑94 (MQ=60)

                                                    CAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAA                                          >  SRR4302533.642566/1‑94 (MQ=60)

                                                    CAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAA                                          <  SRR4302533.2335718/94‑1 (MQ=60)

                                                      GACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATT                                        >  SRR4302533.396172/1‑94 (MQ=60)

                                                       ACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTG                                       >  SRR4302533.2255029/1‑94 (MQ=60)

                                                       ACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTA                                       >  SRR4302533.316610/1‑94 (MQ=60)

                                                        CCACCCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGA                                      <  SRR4302533.2812559/94‑1 (MQ=60)

                                                         CAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAA                                     <  SRR4302533.5157490/94‑1 (MQ=60)

                                                          CACCACATTTCAGAAACCGCATCCTCCTCAACAATTGGCCCCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAA                                    <  SRR4302533.2661937/94‑1 (MQ=60)

                                                              ACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACC                                >  SRR4302533.3367301/1‑94 (MQ=60)

                                                              ACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACC                                <  SRR4302533.2984438/94‑1 (MQ=60)

                                                                ATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCA                              <  SRR4302533.3819374/94‑1 (MQ=60)

                                                                ATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCA                              <  SRR4302533.3190411/94‑1 (MQ=60)

                                                                ATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCA                              <  SRR4302533.3013492/94‑1 (MQ=60)

                                                                 TTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAG                             <  SRR4302533.5376936/94‑1 (MQ=60)

                                                                  TTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACACAGA                            >  SRR4302533.5547123/1‑94 (MQ=60)

                                                                   TCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGAC                           <  SRR4302533.404239/94‑1 (MQ=60)

                                                                     AGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCG                         >  SRR4302533.107924/1‑94 (MQ=60)

                                                                     AGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCG                         >  SRR4302533.1562019/1‑94 (MQ=60)

                                                                          CCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGA                    >  SRR4302533.935128/1‑94 (MQ=60)

                                                                          CCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGA                    >  SRR4302533.2295044/1‑94 (MQ=60)

                                                                            GCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAA                  >  SRR4302533.1383692/1‑94 (MQ=60)

                                                                             CATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAC                 >  SRR4302533.263315/1‑94 (MQ=60)

                                                                              ATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACC                >  SRR4302533.4079360/1‑94 (MQ=60)

                                                                              ATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACA                >  SRR4302533.2145642/1‑94 (MQ=60)

                                                                              ATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACA                >  SRR4302533.3660134/1‑94 (MQ=60)

                                                                                CCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAG              <  SRR4302533.4233519/94‑1 (MQ=60)

                                                                                 CTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGA             >  SRR4302533.1401041/1‑94 (MQ=60)

                                                                                  TCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGAC            <  SRR4302533.604279/94‑1 (MQ=60)

                                                                                  TCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGAC            <  SRR4302533.2137520/94‑1 (MQ=60)

                                                                                  TCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGAC            >  SRR4302533.3016987/1‑94 (MQ=60)

                                                                                  TCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGAC            >  SRR4302533.401870/1‑94 (MQ=60)

                                                                                   CTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACC           <  SRR4302533.1764856/94‑1 (MQ=60)

                                                                                   CTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACC           <  SRR4302533.4334811/94‑1 (MQ=60)

                                                                                      CAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGAT        <  SRR4302533.20044/94‑1 (MQ=60)

                                                                                         CAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGAT     <  SRR4302533.2673852/94‑1 (MQ=60)

                                                                                           ATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGG   <  SRR4302533.3772970/94‑1 (MQ=60)

                                                                                            TTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGT  <  SRR4302533.2980652/94‑1 (MQ=60)

CAGATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGT  >  NC_000913/1666741‑1666926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`