Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,702,726 T→C intergenic (‑394/‑247) b1624 ← / → b1625 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,702,7260TC100.0% 674.8 / NA 208intergenic (‑394/‑247)b1624/b1625hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (78/130);  total (78/130)

Only 100 of 218 total aligned reads displayed.

GCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGT  >  NC_000913/1702636‑1702815
                                                                                          |                                                                                         
 cgtggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATgccgcc                                                                                        <  2:384473/93‑1 (MQ=255)
 cgtggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATgccgcc                                                                                        <  1:948355/93‑1 (MQ=255)
   tggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCg                                                                                      <  2:4053304/93‑1 (MQ=255)
   tggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCg                                                                                      <  2:2009277/93‑1 (MQ=255)
   tggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTACGGTACAACTCAAATAAGCTCTGCACTTGTGGAGCGACCTGCCGCCCg                                                                                      <  2:6299458/93‑1 (MQ=255)
    ggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGt                                                                                     <  2:1229107/93‑1 (MQ=255)
    ggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGt                                                                                     <  2:2154365/93‑1 (MQ=255)
    ggcgAGCCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGt                                                                                     <  2:5496527/93‑1 (MQ=255)
     gcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTc                                                                                    <  1:87255/93‑1 (MQ=255)
      cgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTcc                                                                                   <  1:1943901/93‑1 (MQ=255)
         gTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCggg                                                                                <  1:1524019/93‑1 (MQ=255)
         gTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCggg                                                                                <  2:4450228/93‑1 (MQ=255)
             caTTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCa                                                                            <  2:5160365/93‑1 (MQ=255)
             caTTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCa                                                                            <  1:2612560/93‑1 (MQ=255)
               ttGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATg                                                                          <  2:2572320/93‑1 (MQ=255)
               ttGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATg                                                                          >  2:5114400/1‑93 (MQ=255)
                 gACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATTCCGCCCGTCCGGGTGCTTGtt                                                                        <  2:1001189/93‑1 (MQ=255)
                   cAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGtttt                                                                      >  2:4781736/1‑93 (MQ=255)
                    aaCTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTc                                                                     <  1:3603790/93‑1 (MQ=255)
                    aaCTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTc                                                                     >  1:776198/1‑93 (MQ=255)
                     aCTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCa                                                                    <  2:635981/93‑1 (MQ=255)
                      cTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCAc                                                                   <  1:4694042/93‑1 (MQ=255)
                       tATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACt                                                                  >  1:2262052/1‑93 (MQ=255)
                         tCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTg                                                                >  2:3124445/1‑93 (MQ=255)
                         tCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTg                                                                >  1:2828353/1‑93 (MQ=255)
                         tCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTg                                                                <  1:1907471/93‑1 (MQ=255)
                         tCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTg                                                                >  2:6274923/1‑93 (MQ=255)
                          cAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGt                                                               <  1:2234735/93‑1 (MQ=255)
                            gCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCg                                                             >  2:1202680/1‑93 (MQ=255)
                            gCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCg                                                             >  1:1251543/1‑93 (MQ=255)
                            gCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCg                                                             <  1:6215202/93‑1 (MQ=255)
                               aCCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGat                                                          >  1:4300623/1‑93 (MQ=255)
                                ccTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGata                                                         <  1:4132836/93‑1 (MQ=255)
                                 cTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGatat                                                        >  2:6183754/1‑93 (MQ=255)
                                  tGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATAtt                                                       <  1:4971407/93‑1 (MQ=255)
                                   gAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTa                                                      <  1:1218647/93‑1 (MQ=255)
                                     aCAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTaaa                                                    <  2:184479/93‑1 (MQ=255)
                                     aCAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTaaa                                                    >  2:4456903/1‑93 (MQ=255)
                                     aCAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTaaa                                                    <  1:5771366/93‑1 (MQ=255)
                                       agagTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAAcc                                                  <  1:659685/93‑1 (MQ=255)
                                       agagTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAAcc                                                  <  2:3337115/93‑1 (MQ=255)
                                        gagTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCa                                                 <  2:2037154/93‑1 (MQ=255)
                                         agTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAg                                                <  2:2484666/93‑1 (MQ=255)
                                            gCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGaa                                             <  1:1597480/93‑1 (MQ=255)
                                              ttCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAAtt                                           >  2:4822107/1‑93 (MQ=255)
                                              ttCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAAtt                                           <  2:4013338/93‑1 (MQ=255)
                                               tCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAAttt                                          <  2:532693/93‑1 (MQ=255)
                                               tCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAAttt                                          <  1:5385401/93‑1 (MQ=255)
                                               tCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCACGTTTTCACTTGTCGGATATTAAACCAGGAAttt                                          >  2:4318727/1‑93 (MQ=255)
                                                 ggTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATttat                                        <  2:1401381/93‑1 (MQ=255)
                                                  gTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATttatt                                       >  1:452518/1‑93 (MQ=255)
                                                   tACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATttatta                                      <  2:2083325/93‑1 (MQ=255)
                                                    acaacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTtattat                                     >  1:1794039/1‑93 (MQ=255)
                                                    acaacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTtattat                                     >  1:2452249/1‑93 (MQ=255)
                                                     caacaCAAGTAAGCTCTGCACTTGTGGAGCGTCATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATc                                    <  2:4950897/93‑1 (MQ=255)
                                                     caacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATc                                    >  1:3288559/1‑93 (MQ=255)
                                                     caacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATc                                    <  2:803199/93‑1 (MQ=255)
                                                      aacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCt                                   <  1:4939153/93‑1 (MQ=255)
                                                      aacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCt                                   >  2:5172662/1‑93 (MQ=255)
                                                      aacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCt                                   <  2:3254371/93‑1 (MQ=255)
                                                       acacAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCtt                                  <  1:4781103/93‑1 (MQ=255)
                                                        cacaAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTg                                 >  1:5496431/1‑93 (MQ=255)
                                                        cacaAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTg                                 >  2:2898402/1‑93 (MQ=255)
                                                         acaAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGt                                <  1:5038249/93‑1 (MQ=255)
                                                          caAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGtt                               <  2:4267471/93‑1 (MQ=255)
                                                          caAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGtt                               <  1:2644893/93‑1 (MQ=255)
                                                           aaGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTc                              <  2:4133352/93‑1 (MQ=255)
                                                            aGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCg                             >  2:4495092/1‑93 (MQ=255)
                                                             gTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGa                            <  1:5126641/93‑1 (MQ=255)
                                                             gTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGa                            <  1:3900429/93‑1 (MQ=255)
                                                              tAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAt                           >  2:4623531/1‑93 (MQ=255)
                                                              tAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAt                           <  1:5288042/93‑1 (MQ=255)
                                                                 gCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAtgtt                        <  1:6154650/93‑1 (MQ=255)
                                                                 gCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAtgtt                        >  1:2938099/1‑93 (MQ=255)
                                                                 gCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGCTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAtgtt                        >  1:3120246/1‑93 (MQ=255)
                                                                   tctGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAtgttgt                      >  1:5261944/1‑93 (MQ=255)
                                                                     tGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGttgttg                    >  2:2116114/1‑93 (MQ=255)
                                                                     tGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGttgttg                    >  1:1103913/1‑93 (MQ=255)
                                                                       cACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGt                  >  1:3009263/1‑93 (MQ=255)
                                                                       cACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGt                  <  2:579825/93‑1 (MQ=255)
                                                                        aCTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTg                 >  1:3114349/1‑93 (MQ=255)
                                                                        aCTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTg                 >  2:5880007/1‑93 (MQ=255)
                                                                         cTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGa                <  1:3937967/93‑1 (MQ=255)
                                                                         cTTGTGGAGCGACATGCCGACCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGa                >  2:6139736/1‑93 (MQ=255)
                                                                          ttGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGAt               <  2:1056290/93‑1 (MQ=255)
                                                                          ttGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGAt               <  1:5868771/93‑1 (MQ=255)
                                                                          ttGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGAt               >  1:3716803/1‑93 (MQ=255)
                                                                             tgGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGt            >  2:1717962/1‑93 (MQ=255)
                                                                               gAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCa          <  1:3411228/93‑1 (MQ=255)
                                                                                 gCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAgg        <  2:5023666/93‑1 (MQ=255)
                                                                                  cGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAggg       <  1:3693485/93‑1 (MQ=255)
                                                                                   gACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGa      <  1:2011037/93‑1 (MQ=255)
                                                                                   gACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGa      <  1:3540522/93‑1 (MQ=255)
                                                                                   gACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATGGTCAGGGa      <  2:6123910/93‑1 (MQ=255)
                                                                                    aCATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGAt     <  2:1747564/93‑1 (MQ=255)
                                                                                      aTGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAg   >  1:5701487/1‑93 (MQ=255)
                                                                                      aTGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAg   <  2:3438732/93‑1 (MQ=255)
                                                                                      aTGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAg   <  2:155835/93‑1 (MQ=255)
                                                                                       tgccgcCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGt  <  2:1263973/93‑1 (MQ=255)
                                                                                       tgccgcCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGt  <  1:1442520/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGT  >  NC_000913/1702636‑1702815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAG  >  NC_000913/1702634‑1702819
                                                                                            |                                                                                             
TGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCG                                                                                              >  SRR4302533.3798001/1‑94 (MQ=60)
  GCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCC                                                                                            <  SRR4302533.4935909/94‑1 (MQ=60)
   CGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCC                                                                                           <  SRR4302533.953927/94‑1 (MQ=60)
    GTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCG                                                                                          >  SRR4302533.1657885/1‑94 (MQ=60)
       GCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCC                                                                                       <  SRR4302533.87814/94‑1 (MQ=60)
        CGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCG                                                                                      <  SRR4302533.5845881/94‑1 (MQ=60)
        CGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCG                                                                                      <  SRR4302533.4717443/94‑1 (MQ=60)
        CGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCG                                                                                      <  SRR4302533.1955759/94‑1 (MQ=60)
         GAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGG                                                                                     <  SRR4302533.5788619/94‑1 (MQ=60)
          AGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGG                                                                                    <  SRR4302533.80885/94‑1 (MQ=60)
           GTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGT                                                                                   <  SRR4302533.1533194/94‑1 (MQ=60)
            TCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTG                                                                                  <  SRR4302533.3936771/94‑1 (MQ=60)
              ACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCA                                                                                <  SRR4302533.1250136/94‑1 (MQ=60)
               CATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCAT                                                                               <  SRR4302533.2628595/94‑1 (MQ=60)
                ATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATG                                                                              <  SRR4302533.2283201/94‑1 (MQ=60)
                  TGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTT                                                                            <  SRR4302533.557457/94‑1 (MQ=60)
                   GACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTT                                                                           >  SRR4302533.3136869/1‑94 (MQ=60)
                     CAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTC                                                                         >  SRR4302533.780759/1‑94 (MQ=60)
                     CAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTC                                                                         >  SRR4302533.2010895/1‑94 (MQ=60)
                      AACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCA                                                                        <  SRR4302533.3625970/94‑1 (MQ=60)
                        CTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACT                                                                      <  SRR4302533.4722486/94‑1 (MQ=60)
                        CTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACT                                                                      >  SRR4302533.2276041/1‑94 (MQ=60)
                         TATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTT                                                                     <  SRR4302533.4749229/94‑1 (MQ=60)
                         TATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTT                                                                     >  SRR4302533.1937739/1‑94 (MQ=60)
                          ATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTG                                                                    >  SRR4302533.2845753/1‑94 (MQ=60)
                          ATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTG                                                                    <  SRR4302533.2658863/94‑1 (MQ=60)
                           TCAGGCACCTGAACAGAGTGCTGCGGTACAAGACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGT                                                                   <  SRR4302533.1835907/94‑1 (MQ=60)
                           TCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGT                                                                   <  SRR4302533.1919108/94‑1 (MQ=60)
                           TCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGT                                                                   >  SRR4302533.3793780/1‑94 (MQ=60)
                            CAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTC                                                                  <  SRR4302533.2248522/94‑1 (MQ=60)
                             AGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCG                                                                 >  SRR4302533.1259021/1‑94 (MQ=60)
                             AGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCG                                                                 >  SRR4302533.3737827/1‑94 (MQ=60)
                             AGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCG                                                                 <  SRR4302533.1511112/94‑1 (MQ=60)
                             AGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCG                                                                 >  SRR4302533.4982268/1‑94 (MQ=60)
                              GCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGG                                                                <  SRR4302533.6252470/94‑1 (MQ=60)
                                CACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGAT                                                              <  SRR4302533.6239340/94‑1 (MQ=60)
                                CACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGAT                                                              >  SRR4302533.4326487/1‑94 (MQ=60)
                                CACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGAT                                                              >  SRR4302533.5504652/1‑94 (MQ=60)
                                  CCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATAT                                                            <  SRR4302533.3699237/94‑1 (MQ=60)
                                  CCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATAT                                                            <  SRR4302533.1760955/94‑1 (MQ=60)
                                  CCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATAT                                                            <  SRR4302533.4157926/94‑1 (MQ=60)
                                   CTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATT                                                           >  SRR4302533.3338175/1‑94 (MQ=60)
                                   CTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATT                                                           <  SRR4302533.960410/94‑1 (MQ=60)
                                    TGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTA                                                          <  SRR4302533.5001550/94‑1 (MQ=60)
                                     GAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAA                                                         <  SRR4302533.886057/94‑1 (MQ=60)
                                     GAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAA                                                         <  SRR4302533.1225964/94‑1 (MQ=60)
                                      AACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAA                                                        >  SRR4302533.3008557/1‑94 (MQ=60)
                                       ACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAAC                                                       <  SRR4302533.5806215/94‑1 (MQ=60)
                                         AGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCA                                                     <  SRR4302533.3583559/94‑1 (MQ=60)
                                         AGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCA                                                     <  SRR4302533.663572/94‑1 (MQ=60)
                                         AGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCA                                                     >  SRR4302533.4979397/1‑94 (MQ=60)
                                           AGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGG                                                   >  SRR4302533.359451/1‑94 (MQ=60)
                                             TGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAA                                                 >  SRR4302533.3967606/1‑94 (MQ=60)
                                              GCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAAT                                                <  SRR4302533.1607153/94‑1 (MQ=60)
                                                 TCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTA                                             <  SRR4302533.5417757/94‑1 (MQ=60)
                                                  CGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTAT                                            >  SRR4302533.6223134/1‑94 (MQ=60)
                                                  CGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTAT                                            <  SRR4302533.2964237/94‑1 (MQ=60)
                                                  CGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTAT                                            <  SRR4302533.757276/94‑1 (MQ=60)
                                                   GGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATT                                           >  SRR4302533.455227/1‑94 (MQ=60)
                                                     TACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTAT                                         >  SRR4302533.1804957/1‑94 (MQ=60)
                                                     TACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTAT                                         >  SRR4302533.2467385/1‑94 (MQ=60)
                                                      ACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATC                                        >  SRR4302533.3308859/1‑94 (MQ=60)
                                                       CAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCT                                       <  SRR4302533.4074632/94‑1 (MQ=60)
                                                        AACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTT                                      <  SRR4302533.4969054/94‑1 (MQ=60)
                                                         ACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTG                                     >  SRR4302533.5529583/1‑94 (MQ=60)
                                                         ACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTG                                     >  SRR4302533.5233327/1‑94 (MQ=60)
                                                         ACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTG                                     <  SRR4302533.4810071/94‑1 (MQ=60)
                                                          CACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGT                                    <  SRR4302533.532779/94‑1 (MQ=60)
                                                           ACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTT                                   <  SRR4302533.2953658/94‑1 (MQ=60)
                                                           ACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTT                                   >  SRR4302533.3480236/1‑94 (MQ=60)
                                                           ACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTT                                   <  SRR4302533.657197/94‑1 (MQ=60)
                                                           ACAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTT                                   <  SRR4302533.5068756/94‑1 (MQ=60)
                                                            CAAGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTC                                  <  SRR4302533.2661146/94‑1 (MQ=60)
                                                              AGTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGA                                >  SRR4302533.5294745/1‑94 (MQ=60)
                                                               GTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAT                               <  SRR4302533.3924237/94‑1 (MQ=60)
                                                               GTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAT                               <  SRR4302533.5157611/94‑1 (MQ=60)
                                                               GTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAT                               <  SRR4302533.1278186/94‑1 (MQ=60)
                                                               GTAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAT                               >  SRR4302533.1588113/1‑94 (MQ=60)
                                                                TAAGCTCTGCACTTGTTGAGGGACCTGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATG                              <  SRR4302533.537685/94‑1 (MQ=60)
                                                                TAAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATG                              <  SRR4302533.5319711/94‑1 (MQ=60)
                                                                 AAGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGT                             <  SRR4302533.3155338/94‑1 (MQ=60)
                                                                  AGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTT                            >  SRR4302533.2956054/1‑94 (MQ=60)
                                                                  AGCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGCTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTT                            >  SRR4302533.3139434/1‑94 (MQ=60)
                                                                   GCTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTG                           <  SRR4302533.6191681/94‑1 (MQ=60)
                                                                    CTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGT                          >  SRR4302533.5293533/1‑94 (MQ=60)
                                                                    CTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGT                          <  SRR4302533.5144646/94‑1 (MQ=60)
                                                                    CTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGT                          >  SRR4302533.4443264/1‑94 (MQ=60)
                                                                    CTCTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGT                          >  SRR4302533.4248289/1‑94 (MQ=60)
                                                                      CTGCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTG                        >  SRR4302533.1110451/1‑94 (MQ=60)
                                                                        GCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGT                      <  SRR4302533.5982275/94‑1 (MQ=60)
                                                                        GCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGT                      >  SRR4302533.3027675/1‑94 (MQ=60)
                                                                        GCACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGT                      >  SRR4302533.2251280/1‑94 (MQ=60)
                                                                         CACTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTG                     >  SRR4302533.3133501/1‑94 (MQ=60)
                                                                           CTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGAT                   <  SRR4302533.3962003/94‑1 (MQ=60)
                                                                           CTTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGAT                   >  SRR4302533.3739617/1‑94 (MQ=60)
                                                                            TTGTGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATT                  <  SRR4302533.5904242/94‑1 (MQ=60)
                                                                               TGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTC               <  SRR4302533.1220917/94‑1 (MQ=60)
                                                                               TGGAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTC               <  SRR4302533.5057168/94‑1 (MQ=60)
                                                                                 GAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAG             <  SRR4302533.3432297/94‑1 (MQ=60)
                                                                                 GAGCGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAG             <  SRR4302533.2493867/94‑1 (MQ=60)
                                                                                    CGACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGA          <  SRR4302533.3716174/94‑1 (MQ=60)
                                                                                     GACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGAT         <  SRR4302533.3562337/94‑1 (MQ=60)
                                                                                     GACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGAT         >  SRR4302533.2755729/1‑94 (MQ=60)
                                                                                     GACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGAT         <  SRR4302533.2413751/94‑1 (MQ=60)
                                                                                     GACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGAT         >  SRR4302533.2199778/1‑94 (MQ=60)
                                                                                     GACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGAT         <  SRR4302533.2023426/94‑1 (MQ=60)
                                                                                      ACATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATA        <  SRR4302533.3109866/94‑1 (MQ=60)
                                                                                       CATGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAG       >  SRR4302533.5735917/1‑94 (MQ=60)
                                                                                         TGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTA     <  SRR4302533.1451221/94‑1 (MQ=60)
                                                                                         TGCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTA     <  SRR4302533.969747/94‑1 (MQ=60)
                                                                                          GCCGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAA    <  SRR4302533.2490737/94‑1 (MQ=60)
                                                                                            CGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAG  <  SRR4302533.2787508/94‑1 (MQ=60)
                                                                                            |                                                                                             
TGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAG  >  NC_000913/1702634‑1702819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑