Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,720,316 T→C F58L (TTT→CTT)  b1645 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3160TC100.0% 620.0 / NA 193F58L (TTT→CTT) b1645hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (96/97);  total (96/97)

Only 100 of 199 total aligned reads displayed.

ACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGG  >  NC_000913/1720226‑1720405
                                                                                          |                                                                                         
aCCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCctt                                                                                         >  1:4458110/1‑93 (MQ=255)
 ccATTTCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTc                                                                                        <  2:1775009/93‑1 (MQ=255)
 ccATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTc                                                                                        <  1:1839658/93‑1 (MQ=255)
  cATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTcc                                                                                       >  1:4717054/1‑93 (MQ=255)
  cATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTcc                                                                                       <  1:4844471/93‑1 (MQ=255)
    ttGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCa                                                                                     >  2:2631073/1‑93 (MQ=255)
    ttGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCa                                                                                     <  1:1952538/93‑1 (MQ=255)
     tGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCAc                                                                                    <  1:1114656/93‑1 (MQ=255)
     tGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCAc                                                                                    <  2:1730159/93‑1 (MQ=255)
     tGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCAc                                                                                    <  2:5245668/93‑1 (MQ=255)
      gCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCAcc                                                                                   <  1:542893/93‑1 (MQ=255)
       ccATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCg                                                                                  >  2:3043776/1‑93 (MQ=255)
         aTGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGtt                                                                                <  2:4280421/93‑1 (MQ=255)
         aTGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGtt                                                                                >  2:5851526/1‑93 (MQ=255)
         aTGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGtt                                                                                <  2:330458/93‑1 (MQ=255)
         aTGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGtt                                                                                <  2:4850484/93‑1 (MQ=255)
            tgtCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGc                                                                             <  2:1252938/93‑1 (MQ=255)
            tgtCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGc                                                                             >  1:2528598/1‑93 (MQ=255)
            tgtCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGc                                                                             >  2:4519569/1‑93 (MQ=255)
              tCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCgg                                                                           >  1:1665552/1‑93 (MQ=255)
               cTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGCCCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGgt                                                                          <  1:3085695/93‑1 (MQ=255)
                   cgcTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTa                                                                      <  2:5295111/93‑1 (MQ=255)
                    gcTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTAt                                                                     >  1:2964081/1‑93 (MQ=255)
                     cTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATc                                                                    <  1:5411501/93‑1 (MQ=255)
                      tGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCa                                                                   <  2:3525136/93‑1 (MQ=255)
                       gACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAg                                                                  >  2:3665591/1‑93 (MQ=255)
                         cGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCa                                                                >  2:2290009/1‑93 (MQ=255)
                         cGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCa                                                                >  2:3387841/1‑93 (MQ=255)
                          ggTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCaa                                                               >  1:480619/1‑93 (MQ=255)
                           gTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCaaa                                                              >  2:2557203/1‑93 (MQ=255)
                             tGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAg                                                            >  2:2200485/1‑93 (MQ=255)
                             tGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAg                                                            >  2:1934696/1‑93 (MQ=255)
                              gCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGc                                                           >  2:935720/1‑93 (MQ=255)
                               ccTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGcc                                                          >  2:5019480/1‑93 (MQ=255)
                               ccTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGcc                                                          <  1:2930466/93‑1 (MQ=255)
                               ccTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGcc                                                          >  2:4112378/1‑93 (MQ=255)
                                cTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCt                                                         <  2:3825261/93‑1 (MQ=255)
                                 tattatTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTc                                                        <  2:3127967/93‑1 (MQ=255)
                                  attattTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCg                                                       <  2:4450224/93‑1 (MQ=255)
                                  attattTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCg                                                       <  2:5368203/93‑1 (MQ=255)
                                  attattTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCg                                                       <  1:4114967/93‑1 (MQ=255)
                                  attattTAAATCTCGATGACCCCTCTTTGGCGATGCCCTCGGCTGAAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCg                                                       <  2:888578/93‑1 (MQ=255)
                                   ttattTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCgg                                                      >  1:2245817/1‑93 (MQ=255)
                                    tattTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGa                                                     <  1:3515589/93‑1 (MQ=255)
                                     attTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGAc                                                    <  2:6222342/93‑1 (MQ=255)
                                     attTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGAc                                                    <  1:1543075/93‑1 (MQ=255)
                                      tttAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACg                                                   >  1:5726206/1‑93 (MQ=255)
                                        tAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCa                                                 <  2:2355677/93‑1 (MQ=255)
                                        tAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCa                                                 <  2:3873585/93‑1 (MQ=255)
                                        tAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCa                                                 >  1:1340885/1‑93 (MQ=255)
                                         aaaTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCAt                                                <  1:1367478/93‑1 (MQ=255)
                                          aaTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATc                                               >  2:2443501/1‑93 (MQ=255)
                                          aaTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATc                                               >  1:4304918/1‑93 (MQ=255)
                                          aaTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATc                                               <  2:118515/93‑1 (MQ=255)
                                           aTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCg                                              >  1:4328271/1‑93 (MQ=255)
                                            tCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGc                                             >  1:2575731/1‑93 (MQ=255)
                                            acTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAACCCTCGGACGCATCGc                                             <  2:6002496/92‑1 (MQ=255)
                                             cTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCt                                            <  1:4645926/93‑1 (MQ=255)
                                             cTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCt                                            <  1:2675538/93‑1 (MQ=255)
                                              tGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTg                                           <  1:2049336/93‑1 (MQ=255)
                                              tGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTg                                           <  2:658742/93‑1 (MQ=255)
                                                gATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGc                                         >  1:698584/1‑93 (MQ=255)
                                                 aTGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCa                                        <  1:5909876/93‑1 (MQ=255)
                                                  tGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAg                                       >  2:2110998/1‑93 (MQ=255)
                                                      cccTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTg                                   >  1:4921752/1‑93 (MQ=255)
                                                       ccTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGc                                  <  2:1445351/93‑1 (MQ=255)
                                                       ccTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGc                                  >  2:5381498/1‑93 (MQ=255)
                                                       ccTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGc                                  <  2:4355956/93‑1 (MQ=255)
                                                        cTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGGAGTTTGCt                                 >  1:360910/1‑93 (MQ=255)
                                                         tATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTc                                >  1:1024368/1‑93 (MQ=255)
                                                           ttGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCgg                              <  1:496407/93‑1 (MQ=255)
                                                            tGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGa                             >  2:1330380/1‑93 (MQ=255)
                                                              ggCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGc                           <  1:754983/93‑1 (MQ=255)
                                                                cGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCa                         >  1:5111338/1‑93 (MQ=255)
                                                                 gATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCAt                        >  2:2690339/1‑93 (MQ=255)
                                                                 gATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCAt                        >  1:5822229/1‑93 (MQ=255)
                                                                  aTGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCAtt                       <  1:2283907/93‑1 (MQ=255)
                                                                  aTGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCAtt                       <  1:5785377/93‑1 (MQ=255)
                                                                     aCCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTCTGCTCGGAGCCATTGCg                    >  1:5501249/1‑93 (MQ=255)
                                                                        tCGGCTGCCGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCa                 >  2:6146152/1‑93 (MQ=255)
                                                                        tCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGTGGCa                 >  1:549205/1‑93 (MQ=255)
                                                                         cGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCAc                >  1:270640/1‑93 (MQ=255)
                                                                         cGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCAc                >  1:3779876/1‑93 (MQ=255)
                                                                          ggCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACt               <  2:2381636/93‑1 (MQ=255)
                                                                           gCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTg              >  2:5089376/1‑93 (MQ=255)
                                                                            cTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGc             >  1:467548/1‑93 (MQ=255)
                                                                            cTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGc             <  1:4176058/93‑1 (MQ=255)
                                                                             tGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGct            >  2:4723081/1‑93 (MQ=255)
                                                                               cAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGcttc          <  1:1692144/93‑1 (MQ=255)
                                                                               cAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGcttc          <  2:786923/93‑1 (MQ=255)
                                                                               cAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGcttc          >  1:483161/1‑93 (MQ=255)
                                                                                cgTGGTTAGCGTTCCCACCGTTGGCGGTGTAATCAGCAAAAGCCTCGGACGCAGCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGcttct         <  2:2051974/92‑1 (MQ=255)
                                                                                 gTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGcttctt        >  1:2510280/1‑93 (MQ=255)
                                                                                 gTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGcttctt        >  1:5348635/1‑93 (MQ=255)
                                                                                  tGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTg       <  1:2866517/93‑1 (MQ=255)
                                                                                  tGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTg       <  2:1589809/93‑1 (MQ=255)
                                                                                   ggTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGc      <  1:3187631/93‑1 (MQ=255)
                                                                                    gTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGcc     <  1:2388168/93‑1 (MQ=255)
                                                                                    gTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGcc     <  1:3311257/93‑1 (MQ=255)
                                                                                    gTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGcc     >  1:4692285/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGG  >  NC_000913/1720226‑1720405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATA  >  NC_000913/1720223‑1720409
                                                                                             |                                                                                             
AATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCC                                                                                               >  SRR4302533.5023664/1‑94 (MQ=60)
 ATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCT                                                                                              >  SRR4302533.2168747/1‑94 (MQ=60)
  TACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGCCCTCGGCTGCAGTGGTTAGCCTT                                                                                             <  SRR4302533.1289213/94‑1 (MQ=60)
  TACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTT                                                                                             >  SRR4302533.4485074/1‑94 (MQ=60)
  AACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTT                                                                                             <  SRR4302533.5300421/94‑1 (MQ=60)
    CCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCC                                                                                           >  SRR4302533.4745654/1‑94 (MQ=60)
    CCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCC                                                                                           <  SRR4302533.1850878/94‑1 (MQ=60)
     CATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCC                                                                                          >  SRR4302533.4617388/1‑94 (MQ=60)
     CATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCC                                                                                          <  SRR4302533.4873834/94‑1 (MQ=60)
      ATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCA                                                                                         <  SRR4302533.2543558/94‑1 (MQ=60)
       TTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCAC                                                                                        <  SRR4302533.1964439/94‑1 (MQ=60)
        TGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACC                                                                                       <  SRR4302533.1121265/94‑1 (MQ=60)
        TGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACC                                                                                       <  SRR4302533.697838/94‑1 (MQ=60)
        TGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCATATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACC                                                                                       <  SRR4302533.3125478/94‑1 (MQ=60)
         GCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCG                                                                                      <  SRR4302533.546116/94‑1 (MQ=60)
             TGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGG                                                                                  <  SRR4302533.2291728/94‑1 (MQ=60)
              GTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGC                                                                                 >  SRR4302533.2544166/1‑94 (MQ=60)
                GTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGG                                                                               >  SRR4302533.1675616/1‑94 (MQ=60)
                GTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGG                                                                               >  SRR4302533.5586047/1‑94 (MQ=60)
                 TCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGT                                                                              <  SRR4302533.336796/94‑1 (MQ=60)
                  CTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGCCCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTG                                                                             <  SRR4302533.3104666/94‑1 (MQ=60)
                   TGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGT                                                                            >  SRR4302533.4979198/1‑94 (MQ=60)
                      CGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTAT                                                                         <  SRR4302533.2722834/94‑1 (MQ=60)
                      CGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTAT                                                                         >  SRR4302533.2982200/1‑94 (MQ=60)
                       GCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATC                                                                        <  SRR4302533.4780538/94‑1 (MQ=60)
                        CTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCA                                                                       >  SRR4302533.2416332/1‑94 (MQ=60)
                        CTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCA                                                                       <  SRR4302533.5444037/94‑1 (MQ=60)
                        CTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCA                                                                       >  SRR4302533.5530474/1‑94 (MQ=60)
                            CGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAA                                                                   >  SRR4302533.483492/1‑94 (MQ=60)
                            CGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAA                                                                   >  SRR4302533.2845282/1‑94 (MQ=60)
                               TTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGAGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAC                                                                >  SRR4302533.2680949/1‑94 (MQ=60)
                                 GCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCC                                                              >  SRR4302533.740433/1‑94 (MQ=60)
                                 GCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCC                                                              >  SRR4302533.258247/1‑94 (MQ=60)
                                  CCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCT                                                             <  SRR4302533.2948390/94‑1 (MQ=60)
                                  CCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGACTTCCCACCGTTGGCGGTGTTATCAGCAAAAGacc                                                             >  SRR4302533.1678288/1‑91 (MQ=60)
                                   CTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGGCTC                                                            >  SRR4302533.4365117/1‑94 (MQ=60)
                                   CTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTC                                                            <  SRR4302533.3542344/94‑1 (MQ=60)
                                   CTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTC                                                            <  SRR4302533.1671148/94‑1 (MQ=60)
                                   CTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTC                                                            >  SRR4302533.3885303/1‑94 (MQ=60)
                                    TATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCG                                                           <  SRR4302533.4241945/94‑1 (MQ=60)
                                    TATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCG                                                           <  SRR4302533.3090507/94‑1 (MQ=60)
                                     ATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGG                                                          >  SRR4302533.2259695/1‑94 (MQ=60)
                                     ATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGG                                                          <  SRR4302533.4139971/94‑1 (MQ=60)
                                      TTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGA                                                         <  SRR4302533.4900910/94‑1 (MQ=60)
                                       TATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGAC                                                        <  SRR4302533.5811492/94‑1 (MQ=60)
                                       TATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGAC                                                        <  SRR4302533.3537259/94‑1 (MQ=60)
                                        ATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACG                                                       >  SRR4302533.5760777/1‑94 (MQ=60)
                                        ATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACG                                                       <  SRR4302533.1552368/94‑1 (MQ=60)
                                          TTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCA                                                     >  SRR4302533.1348958/1‑94 (MQ=60)
                                            AAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATC                                                   <  SRR4302533.5453930/94‑1 (MQ=60)
                                            AAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATC                                                   <  SRR4302533.1375700/94‑1 (MQ=60)
                                            AAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATC                                                   >  SRR4302533.4330819/1‑94 (MQ=60)
                                             AATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCG                                                  >  SRR4302533.4354346/1‑94 (MQ=60)
                                             AATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCG                                                  >  SRR4302533.5132873/1‑94 (MQ=60)
                                             AATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCG                                                  <  SRR4302533.4837086/94‑1 (MQ=60)
                                             AATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCG                                                  <  SRR4302533.2966597/94‑1 (MQ=60)
                                              ATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCACAAGCCTCGGACGCATCGC                                                 >  SRR4302533.4688006/1‑94 (MQ=60)
                                              ATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGC                                                 >  SRR4302533.2591577/1‑94 (MQ=60)
                                                CTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTG                                               <  SRR4302533.5510166/94‑1 (MQ=60)
                                                CTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTG                                               <  SRR4302533.4674082/94‑1 (MQ=60)
                                                CTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTG                                               <  SRR4302533.2691948/94‑1 (MQ=60)
                                                 TGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGG                                              <  SRR4302533.2061977/94‑1 (MQ=60)
                                                  GGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGC                                             >  SRR4302533.702716/1‑94 (MQ=60)
                                                   GATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCA                                            <  SRR4302533.3249290/94‑1 (MQ=60)
                                                    ATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAG                                           <  SRR4302533.5945575/94‑1 (MQ=60)
                                                     TGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGT                                          <  SRR4302533.1871650/94‑1 (MQ=60)
                                                      GAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTT                                         >  SRR4302533.1278596/1‑94 (MQ=60)
                                                        ACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTG                                       >  SRR4302533.4951565/1‑94 (MQ=60)
                                                          CCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGGAGTTTGCT                                     >  SRR4302533.363106/1‑94 (MQ=60)
                                                           CTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTC                                    >  SRR4302533.1030390/1‑94 (MQ=60)
                                                           CTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTC                                    >  SRR4302533.582014/1‑94 (MQ=60)
                                                             ATTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGG                                  <  SRR4302533.1995857/94‑1 (MQ=60)
                                                              TTGGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGA                                 <  SRR4302533.499368/94‑1 (MQ=60)
                                                                GGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGC                               >  SRR4302533.931627/1‑94 (MQ=60)
                                                                GGGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGC                               <  SRR4302533.2906536/94‑1 (MQ=60)
                                                                 GGCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCC                              <  SRR4302533.759425/94‑1 (MQ=60)
                                                                  GCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCA                             >  SRR4302533.5142230/1‑94 (MQ=60)
                                                                  GCGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCA                             >  SRR4302533.3644449/1‑94 (MQ=60)
                                                                   CGATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCAT                            >  SRR4302533.5857398/1‑94 (MQ=60)
                                                                    GAGGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATT                           <  SRR4302533.4157013/94‑1 (MQ=60)
                                                                     ATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTG                          <  SRR4302533.2298045/94‑1 (MQ=60)
                                                                     ATGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTG                          <  SRR4302533.5820317/94‑1 (MQ=60)
                                                                      TGACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGC                         <  SRR4302533.1355331/94‑1 (MQ=60)
                                                                       GACCTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTCTGCTCGGAGCCATTGCG                        >  SRR4302533.5534435/1‑94 (MQ=60)
                                                                          CTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGTGGCA                     >  SRR4302533.552462/1‑94 (MQ=60)
                                                                          CTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCA                     >  SRR4302533.5865958/1‑94 (MQ=60)
                                                                          CTCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCA                     >  SRR4302533.4709791/1‑94 (MQ=60)
                                                                           TCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCAC                    >  SRR4302533.3803033/1‑94 (MQ=60)
                                                                           TCGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCAC                    >  SRR4302533.272283/1‑94 (MQ=60)
                                                                            CGGCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACT                   <  SRR4302533.2150220/94‑1 (MQ=60)
                                                                              GCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGC                 >  SRR4302533.1828197/1‑94 (MQ=60)
                                                                              GCTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGC                 >  SRR4302533.470340/1‑94 (MQ=60)
                                                                               CTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCT                <  SRR4302533.4201360/94‑1 (MQ=60)
                                                                               CTGCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCT                <  SRR4302533.5394954/94‑1 (MQ=60)
                                                                               CTGCAGTGGTGAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCT                <  SRR4302533.4346679/94‑1 (MQ=60)
                                                                                TGCAGTGGTTAGCCTTCCCACCGTTGGTGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTT               <  SRR4302533.4868750/94‑1 (MQ=60)
                                                                                 GCAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTC              >  SRR4302533.486044/1‑94 (MQ=60)
                                                                                  CAGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCT             <  SRR4302533.1702393/94‑1 (MQ=60)
                                                                                   AGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTT            >  SRR4302533.5380725/1‑94 (MQ=60)
                                                                                   AGTGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTT            >  SRR4302533.2525750/1‑94 (MQ=60)
                                                                                     TGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGC          <  SRR4302533.2884153/94‑1 (MQ=60)
                                                                                     TGGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGC          >  SRR4302533.2325540/1‑94 (MQ=60)
                                                                                      GGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCC         <  SRR4302533.3207240/94‑1 (MQ=60)
                                                                                      GGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCC         <  SRR4302533.4726599/94‑1 (MQ=60)
                                                                                      GGTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCC         >  SRR4302533.4720724/1‑94 (MQ=60)
                                                                                       GTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCG        <  SRR4302533.2402922/94‑1 (MQ=60)
                                                                                       GTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCG        <  SRR4302533.3331704/94‑1 (MQ=60)
                                                                                       GTTAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCG        <  SRR4302533.5225093/94‑1 (MQ=60)
                                                                                         TAGCCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGG      >  SRR4302533.1381775/1‑94 (MQ=60)
                                                                                            CCTTCCCACCGTTGGCGGTGTTATCAGCAAAATCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCAT   <  SRR4302533.822554/94‑1 (MQ=60)
                                                                                            CCTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCAT   >  SRR4302533.4074329/1‑94 (MQ=60)
                                                                                             CTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATA  >  SRR4302533.3307188/1‑94 (MQ=60)
                                                                                             CTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATA  >  SRR4302533.6287610/1‑94 (MQ=60)
                                                                                             CTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATA  >  SRR4302533.3332756/1‑94 (MQ=60)
                                                                                             CTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATA  >  SRR4302533.3943314/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATACCATTGCCATGTGTCTGGCGCTGACGGTTGCCTATTATTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATA  >  NC_000913/1720223‑1720409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑