Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,789,635 T→C Y137C (TAT→TGT)  b1707 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,789,6350TC100.0% 673.9 / NA 205Y137C (TAT→TGT) b1707hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (90/115);  total (90/115)

Only 100 of 212 total aligned reads displayed.

TTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGA  >  NC_000913/1789546‑1789724
                                                                                         |                                                                                         
   tATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACtt                                                                                     <  1:6281975/93‑1 (MQ=255)
    aTCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTg                                                                                    >  2:5455387/1‑93 (MQ=255)
      ccAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCg                                                                                  <  1:5812993/93‑1 (MQ=255)
       cAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGa                                                                                 >  1:4018126/1‑93 (MQ=255)
       cAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGa                                                                                 <  2:4931002/93‑1 (MQ=255)
       cAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGa                                                                                 <  1:3890590/93‑1 (MQ=255)
        aGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGAt                                                                                <  1:1564924/93‑1 (MQ=255)
        aGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGAt                                                                                <  2:1478293/93‑1 (MQ=255)
         gCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATa                                                                               >  1:1896073/1‑93 (MQ=255)
           aTGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAg                                                                             >  2:2595914/1‑93 (MQ=255)
            tGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAgg                                                                            >  2:1643712/1‑93 (MQ=255)
            tGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAgg                                                                            <  2:386478/93‑1 (MQ=255)
             gACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGa                                                                           <  2:5885929/93‑1 (MQ=255)
             gACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGa                                                                           <  1:2974835/93‑1 (MQ=255)
             gACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGa                                                                           >  2:5735600/1‑93 (MQ=255)
             gACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGa                                                                           <  1:4644794/93‑1 (MQ=255)
              aCGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGAt                                                                          <  2:1889300/93‑1 (MQ=255)
                gcgGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATaa                                                                        <  1:6183331/93‑1 (MQ=255)
                 cgGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATaaa                                                                       <  2:1103977/93‑1 (MQ=255)
                 cgGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATaaa                                                                       >  2:2843214/1‑93 (MQ=255)
                   ggTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAg                                                                     <  1:3178139/93‑1 (MQ=255)
                   ggTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAg                                                                     >  2:3476980/1‑93 (MQ=255)
                    gTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGa                                                                    >  1:676610/1‑93 (MQ=255)
                     tGAAAAGACCATGAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAAGGGACCAACCTTCCATTAGGATAAAAAAc                                                                   >  2:5043696/1‑93 (MQ=255)
                     tGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGAc                                                                   <  1:3791733/93‑1 (MQ=255)
                     tGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGAc                                                                   >  1:5037872/1‑93 (MQ=255)
                      gAAAAGACCATCAAAACCCGCTTTCATTTTGATATTGCCTGCTCCCAAAATACCTACCCCAAGGCGACACACTTGCGATAAGGATAAAAGAcc                                                                  <  2:5907502/93‑1 (MQ=255)
                      gAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGAcc                                                                  <  1:1820550/93‑1 (MQ=255)
                      gAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGAcc                                                                  >  1:4289086/1‑93 (MQ=255)
                       aaaaGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACcg                                                                 >  1:2363084/1‑93 (MQ=255)
                        aaaGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACcgc                                                                >  2:4639085/1‑93 (MQ=255)
                           gACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGAt                                                             >  1:3317793/1‑93 (MQ=255)
                            aCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGAtt                                                            <  2:5385106/93‑1 (MQ=255)
                            aCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGAtt                                                            <  1:3993165/93‑1 (MQ=255)
                            aCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGAtt                                                            >  2:6171404/1‑93 (MQ=255)
                             ccATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTa                                                           <  2:2152074/93‑1 (MQ=255)
                              cATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTAt                                                          >  1:4593088/1‑93 (MQ=255)
                                tCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCt                                                        <  2:955875/93‑1 (MQ=255)
                                  taaaaCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTGCCTAACACAAGGGGACTCACTTGCAATAAGGATAAAAGACCGCGATTAGCttt                                                      <  2:6250797/92‑1 (MQ=255)
                                  aaaaaCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCttt                                                      <  2:2503251/93‑1 (MQ=255)
                                   aaaaCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCtttt                                                     <  1:5539596/93‑1 (MQ=255)
                                   aaaaCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCtttt                                                     <  2:72660/93‑1 (MQ=255)
                                   aaaaCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCtttt                                                     <  1:2473952/93‑1 (MQ=255)
                                     aaCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTcc                                                   >  2:3357998/1‑93 (MQ=255)
                                      aCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCt                                                  >  2:3246637/1‑93 (MQ=255)
                                      aCCGCTGTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCt                                                  <  2:5562377/93‑1 (MQ=255)
                                       ccGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCtt                                                 >  2:93729/1‑93 (MQ=255)
                                       ccGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCtt                                                 <  1:1448919/93‑1 (MQ=255)
                                           tttCATTGTGCTATTGTCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCAtt                                             >  2:2207797/1‑93 (MQ=255)
                                           tttCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGCCCGCGATTATCTTTTCCTTCAtt                                             <  1:5334815/93‑1 (MQ=255)
                                           tttCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTTCTTCAtt                                             >  2:1358260/1‑93 (MQ=255)
                                           tttCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCAtt                                             <  2:1202562/93‑1 (MQ=255)
                                             tCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCTTTCATTga                                           <  2:6106874/93‑1 (MQ=255)
                                               aTTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgaga                                         <  1:5611509/93‑1 (MQ=255)
                                                  gtgCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGc                                      <  1:904032/93‑1 (MQ=255)
                                                  gtgCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGc                                      <  1:2856447/93‑1 (MQ=255)
                                                     cTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCggg                                   <  1:1973991/93‑1 (MQ=255)
                                                      tATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGCGATGCGGGt                                  >  1:6019638/1‑93 (MQ=255)
                                                      tATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCggga                                  >  1:4148572/1‑92 (MQ=255)
                                                      tATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGt                                  <  1:340994/93‑1 (MQ=255)
                                                       aTTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTa                                 <  2:302701/93‑1 (MQ=255)
                                                        ttGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTaa                                <  2:1756485/93‑1 (MQ=255)
                                                        ttGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTaa                                <  1:2097682/93‑1 (MQ=255)
                                                        ttGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTaa                                <  1:3717760/93‑1 (MQ=255)
                                                           ccTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtc                             >  2:1056261/1‑93 (MQ=255)
                                                           ccTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtc                             >  1:1787170/1‑93 (MQ=255)
                                                           ccTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtc                             <  2:5167574/93‑1 (MQ=255)
                                                            cTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtct                            >  2:394503/1‑93 (MQ=255)
                                                             tGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtctc                           >  2:3960941/1‑93 (MQ=255)
                                                              gCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCa                          >  2:3695699/1‑93 (MQ=255)
                                                              gCTCCCAAATTACCTAACACAAGGAGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCa                          <  1:5091568/93‑1 (MQ=255)
                                                              gCTCCCAAATTACCTAACACAAGAGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCa                          <  1:2739397/93‑1 (MQ=255)
                                                                tCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCAtt                        <  1:3682621/93‑1 (MQ=255)
                                                                tCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCAtt                        >  1:5100246/1‑93 (MQ=255)
                                                                 cccAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTa                       >  1:4732761/1‑93 (MQ=255)
                                                                  ccAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTaa                      <  1:3014311/93‑1 (MQ=255)
                                                                  ccAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTaa                      >  2:2623386/1‑93 (MQ=255)
                                                                   cAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAAt                     <  1:1047380/93‑1 (MQ=255)
                                                                    aaaTTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATc                    <  2:4092521/93‑1 (MQ=255)
                                                                       ttACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAAc                 >  2:1020173/1‑93 (MQ=255)
                                                                       ttACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAAc                 >  2:5213086/1‑93 (MQ=255)
                                                                       ttACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAAc                 <  2:2006798/93‑1 (MQ=255)
                                                                        tACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACa                >  1:5554124/1‑93 (MQ=255)
                                                                         aCCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAg               <  2:420917/93‑1 (MQ=255)
                                                                         aCCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAg               <  2:4426324/93‑1 (MQ=255)
                                                                         aCCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTCCATTGAGATGCGGGTAATTCTCATTAATCAACAg               <  1:5383942/93‑1 (MQ=255)
                                                                          ccTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGt              <  1:1618024/93‑1 (MQ=255)
                                                                          ccTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGt              <  2:3198239/93‑1 (MQ=255)
                                                                           cTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGtt             >  1:424015/1‑93 (MQ=255)
                                                                            tAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTc            <  2:4553137/93‑1 (MQ=255)
                                                                               cacaAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAAt         <  1:3493133/93‑1 (MQ=255)
                                                                               cacaAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAAt         <  1:4781147/93‑1 (MQ=255)
                                                                               cacaAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGGTCAAt         >  2:3530255/1‑93 (MQ=255)
                                                                                acaAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATa        <  1:5419508/93‑1 (MQ=255)
                                                                                acaAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATa        <  2:3345764/93‑1 (MQ=255)
                                                                                  aaGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATaaa      >  1:609601/1‑93 (MQ=255)
                                                                                  aaGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATaaa      >  1:6253058/1‑93 (MQ=255)
                                                                                  aaGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATaaa      <  2:4791374/93‑1 (MQ=255)
                                                                                     gggACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACgg   <  2:1798574/93‑1 (MQ=255)
                                                                                      ggACACACTTGCGATAAGGATAAAACACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGa  <  1:503484/93‑1 (MQ=255)
                                                                                         |                                                                                         
TTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGA  >  NC_000913/1789546‑1789724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATATT  >  NC_000913/1789547‑1789728
                                                                                        |                                                                                             
TTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACT                                                                                          <  SRR4302533.2748386/94‑1 (MQ=60)
  TATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTG                                                                                        <  SRR4302533.6319468/94‑1 (MQ=60)
    TCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCG                                                                                      <  SRR4302533.5102556/94‑1 (MQ=60)
     CCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGA                                                                                     >  SRR4302533.4042590/1‑94 (MQ=60)
     CCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGA                                                                                     <  SRR4302533.5848101/94‑1 (MQ=60)
      CAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGAT                                                                                    <  SRR4302533.2459344/94‑1 (MQ=60)
      CAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGAT                                                                                    <  SRR4302533.3914327/94‑1 (MQ=60)
       AGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATA                                                                                   <  SRR4302533.1574345/94‑1 (MQ=60)
       AGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATA                                                                                   >  SRR4302533.1907644/1‑94 (MQ=60)
        GCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAA                                                                                  >  SRR4302533.5427714/1‑94 (MQ=60)
        GCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAA                                                                                  <  SRR4302533.617990/94‑1 (MQ=60)
          ATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGG                                                                                >  SRR4302533.5127914/1‑94 (MQ=60)
          ATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGG                                                                                <  SRR4302533.5769646/94‑1 (MQ=60)
            GACGCGGGTGGAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGAT                                                                              >  SRR4302533.2951632/1‑94 (MQ=60)
            GACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGAT                                                                              <  SRR4302533.4672940/94‑1 (MQ=60)
            GACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGAT                                                                              <  SRR4302533.2993037/94‑1 (MQ=60)
               GCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAA                                                                           <  SRR4302533.564167/94‑1 (MQ=60)
               GCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAA                                                                           <  SRR4302533.6220457/94‑1 (MQ=60)
                CGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAA                                                                          >  SRR4302533.4049462/1‑94 (MQ=60)
                CGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAA                                                                          >  SRR4302533.2190168/1‑94 (MQ=60)
                 GGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAG                                                                         >  SRR4302533.3778391/1‑94 (MQ=60)
                  GGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGA                                                                        >  SRR4302533.680609/1‑94 (MQ=60)
                  GGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGA                                                                        <  SRR4302533.3197687/94‑1 (MQ=60)
                   GTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGAC                                                                       >  SRR4302533.5068379/1‑94 (MQ=60)
                    TGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACC                                                                      <  SRR4302533.5011930/94‑1 (MQ=60)
                    TGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACC                                                                      >  SRR4302533.4314878/1‑94 (MQ=60)
                    TGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACC                                                                      <  SRR4302533.3814946/94‑1 (MQ=60)
                     GAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCG                                                                     >  SRR4302533.2377703/1‑94 (MQ=60)
                     GAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCG                                                                     >  SRR4302533.5779242/1‑94 (MQ=60)
                     GAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCG                                                                     <  SRR4302533.1831671/94‑1 (MQ=60)
                      AAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGC                                                                    >  SRR4302533.4749762/1‑94 (MQ=60)
                         AGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGAT                                                                 >  SRR4302533.2809008/1‑94 (MQ=60)
                         AGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGAT                                                                 >  SRR4302533.3338285/1‑94 (MQ=60)
                           ACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTA                                                               <  SRR4302533.4017477/94‑1 (MQ=60)
                           ACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTA                                                               <  SRR4302533.1853221/94‑1 (MQ=60)
                            CCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTAT                                                              >  SRR4302533.4620910/1‑94 (MQ=60)
                            CCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTAT                                                              <  SRR4302533.5065993/94‑1 (MQ=60)
                              ATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGCTTATCT                                                            >  SRR4302533.1527932/1‑94 (MQ=60)
                                CAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTT                                                          >  SRR4302533.3061162/1‑94 (MQ=60)
                                 AAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTT                                                         >  SRR4302533.3546783/1‑94 (MQ=60)
                                  AAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTC                                                        <  SRR4302533.5573047/94‑1 (MQ=60)
                                  AAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTC                                                        <  SRR4302533.2489207/94‑1 (MQ=60)
                                      CCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTC                                                    <  SRR4302533.1457660/94‑1 (MQ=60)
                                          TTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGCCCGCGATTATCTTTTCCTTCATTG                                                <  SRR4302533.5366793/94‑1 (MQ=60)
                                           TTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGA                                               <  SRR4302533.403267/94‑1 (MQ=60)
                                             CATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGA                                             >  SRR4302533.4160088/1‑94 (MQ=60)
                                              ATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAT                                            >  SRR4302533.4189592/1‑94 (MQ=60)
                                              ATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAT                                            <  SRR4302533.5645426/94‑1 (MQ=60)
                                              ATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAT                                            <  SRR4302533.990189/94‑1 (MQ=60)
                                                TGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGC                                          >  SRR4302533.5403868/1‑94 (MQ=60)
                                                TGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGC                                          <  SRR4302533.5533537/94‑1 (MQ=60)
                                                TGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGC                                          >  SRR4302533.713722/1‑94 (MQ=60)
                                                 GTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCG                                         <  SRR4302533.3388047/94‑1 (MQ=60)
                                                 GTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCG                                         <  SRR4302533.2874023/94‑1 (MQ=60)
                                                 GTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCG                                         <  SRR4302533.909333/94‑1 (MQ=60)
                                                  TGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGG                                        >  SRR4302533.1074257/1‑94 (MQ=60)
                                                   GCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGG                                       >  SRR4302533.3224591/1‑94 (MQ=60)
                                                    CTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGCGATGCGGGT                                      >  SRR4302533.6056115/1‑94 (MQ=60)
                                                    CTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGT                                      <  SRR4302533.1986077/94‑1 (MQ=60)
                                                    CTATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGA                                      >  SRR4302533.4173708/1‑94 (MQ=60)
                                                     TATTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTA                                     <  SRR4302533.343077/94‑1 (MQ=60)
                                                       TTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAAT                                   <  SRR4302533.2110596/94‑1 (MQ=60)
                                                       TTGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAAT                                   <  SRR4302533.3740579/94‑1 (MQ=60)
                                                        TGCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATT                                  <  SRR4302533.5852616/94‑1 (MQ=60)
                                                         GCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTC                                 >  SRR4302533.1798033/1‑94 (MQ=60)
                                                         GCCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTC                                 >  SRR4302533.1798032/1‑94 (MQ=60)
                                                          CCTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCT                                >  SRR4302533.4291828/1‑94 (MQ=60)
                                                           CTGCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTC                               >  SRR4302533.5579570/1‑94 (MQ=60)
                                                             GCTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCAT                             <  SRR4302533.1796831/94‑1 (MQ=60)
                                                             GCTCCCAAATTACCTAACACAAGGAGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCAT                             <  SRR4302533.5122327/94‑1 (MQ=60)
                                                             GCTCCCAAATTACCTAACACAAGAGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCAT                             <  SRR4302533.2756194/94‑1 (MQ=60)
                                                              CTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATT                            >  SRR4302533.3723357/1‑94 (MQ=60)
                                                              CTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATT                            >  SRR4302533.5131056/1‑94 (MQ=60)
                                                              CTCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATT                            >  SRR4302533.1609853/1‑94 (MQ=60)
                                                               TCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTA                           >  SRR4302533.4761452/1‑94 (MQ=60)
                                                               TCCCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTA                           <  SRR4302533.3705256/94‑1 (MQ=60)
                                                                 CCAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAAT                         <  SRR4302533.3032746/94‑1 (MQ=60)
                                                                  CAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATC                        >  SRR4302533.67076/1‑94 (MQ=60)
                                                                  CAAATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATC                        <  SRR4302533.1053535/94‑1 (MQ=60)
                                                                    AATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAA                      <  SRR4302533.6237858/94‑1 (MQ=60)
                                                                    AATTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAA                      <  SRR4302533.6302356/94‑1 (MQ=60)
                                                                      TTACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACA                    >  SRR4302533.5587665/1‑94 (MQ=60)
                                                                      TTACCTAACACAAGGGGACACACTCGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACA                    <  SRR4302533.4637552/94‑1 (MQ=60)
                                                                       TACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAG                   <  SRR4302533.3922013/94‑1 (MQ=60)
                                                                        ACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGT                  <  SRR4302533.963301/94‑1 (MQ=60)
                                                                        ACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGT                  <  SRR4302533.5227556/94‑1 (MQ=60)
                                                                        ACCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTCCATTGAGATGCGGGTAATTCTCATTAATCAACAGT                  <  SRR4302533.5416288/94‑1 (MQ=60)
                                                                         CCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTT                 >  SRR4302533.426550/1‑94 (MQ=60)
                                                                         CCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTT                 <  SRR4302533.1627827/94‑1 (MQ=60)
                                                                         CCTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTT                 >  SRR4302533.1925753/1‑94 (MQ=60)
                                                                          CTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTC                >  SRR4302533.4553471/1‑94 (MQ=60)
                                                                          CTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTC                <  SRR4302533.349002/94‑1 (MQ=60)
                                                                          CTAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAGGCGGGTAATTCTCATTAATCAACAGTTC                >  SRR4302533.6096510/1‑94 (MQ=60)
                                                                           TAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCA               <  SRR4302533.3840692/94‑1 (MQ=60)
                                                                           TAACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCA               >  SRR4302533.2236220/1‑94 (MQ=60)
                                                                             ACACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAAT             <  SRR4302533.2035681/94‑1 (MQ=60)
                                                                              CACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATA            >  SRR4302533.4768413/1‑94 (MQ=60)
                                                                              CACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATA            <  SRR4302533.4810116/94‑1 (MQ=60)
                                                                              CACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATA            <  SRR4302533.3514676/94‑1 (MQ=60)
                                                                               ACAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAA           <  SRR4302533.5452113/94‑1 (MQ=60)
                                                                                CAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAA          >  SRR4302533.613186/1‑94 (MQ=60)
                                                                                CAAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAA          >  SRR4302533.6290460/1‑94 (MQ=60)
                                                                                 AAGGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAAC         <  SRR4302533.3007764/94‑1 (MQ=60)
                                                                                   GGGGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGG       <  SRR4302533.336876/94‑1 (MQ=60)
                                                                                     GGACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGAT     <  SRR4302533.5113098/94‑1 (MQ=60)
                                                                                     GGACACACTTGCGATAAGGATAAAACACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGAT     <  SRR4302533.506473/94‑1 (MQ=60)
                                                                                      GACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATA    >  SRR4302533.3784884/1‑94 (MQ=60)
                                                                                       ACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATAT   <  SRR4302533.3202143/94‑1 (MQ=60)
                                                                                       ACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATAT   <  SRR4302533.4346156/94‑1 (MQ=60)
                                                                                       ACACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATAT   >  SRR4302533.5706200/1‑94 (MQ=60)
                                                                                        CACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATATT  <  SRR4302533.3472068/94‑1 (MQ=60)
                                                                                        CACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATATT  >  SRR4302533.4542483/1‑94 (MQ=60)
                                                                                        |                                                                                             
TTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCATTAATCAACAGTTCAATAAACGGATATT  >  NC_000913/1789547‑1789728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑