Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,089,888	T→C	L257P (CTC→CCC)	hisD →	L‑histidinal:NAD+ oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,089,888	0	T	C	100.0%	591.3 / NA	182	L257P (CTC→CCC)	hisD	L‑histidinal:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (86/96); total (86/96)

Only 100 of 189 total aligned reads displayed.

TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC  >  NC_000913/2089799‑2089977

tGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctc                                                                                        <  1:817253/93‑1 (MQ=255)

 gcggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctct                                                                                       >  1:5955710/1‑93 (MQ=255)

  cggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCcctctc                                                                                      <  1:3086046/93‑1 (MQ=255)

    gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  2:5095867/93‑1 (MQ=255)

      gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  >  1:4247646/1‑93 (MQ=255)

      gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  >  1:5630845/1‑93 (MQ=255)

       atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                 >  1:3074609/1‑93 (MQ=255)

         cgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGa                                                                               <  2:2322441/93‑1 (MQ=255)

          gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                              <  2:6296780/93‑1 (MQ=255)

          gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                              >  1:3991095/1‑93 (MQ=255)

           atatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAac                                                                             >  2:1627505/1‑93 (MQ=255)

             atGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAacac                                                                           >  2:1496716/1‑93 (MQ=255)

              tGCCCGCAGGCCCGTCGGAAGTGCTGGTAATTGCTGACAGCGGCGCTAAGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACg                                                                          <  1:6305256/93‑1 (MQ=255)

                 ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                       >  1:2726914/1‑93 (MQ=255)

                 ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                       <  2:2373289/93‑1 (MQ=255)

                  cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                      >  2:1148032/1‑93 (MQ=255)

                  cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                      >  2:1897577/1‑93 (MQ=255)

                     aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACCCGGCCCGGa                                                                   >  1:2055653/1‑93 (MQ=255)

                     aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGa                                                                   >  2:4344790/1‑93 (MQ=255)

                        cccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTc                                                                >  2:5153164/1‑93 (MQ=255)

                         ccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTca                                                               <  1:6222347/93‑1 (MQ=255)

                         ccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTca                                                               >  1:1268761/1‑93 (MQ=255)

                          cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcac                                                              >  2:5756201/1‑93 (MQ=255)

                          cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcac                                                              >  2:842697/1‑93 (MQ=255)

                           gTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcaca                                                             >  2:4130063/1‑93 (MQ=255)

                           gTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcaca                                                             >  1:730385/1‑93 (MQ=255)

                            tCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                            <  2:2558467/93‑1 (MQ=255)

                            gcGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                            <  1:1767668/92‑1 (MQ=255)

                             cGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAgg                                                           <  2:2943553/93‑1 (MQ=255)

                              ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAggg                                                          >  2:3870578/1‑92 (MQ=255)

                               gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTg                                                         >  2:6075328/1‑93 (MQ=255)

                                aaGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGa                                                        <  2:4063806/93‑1 (MQ=255)

                                 aGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGGCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAt                                                       <  1:2893056/93‑1 (MQ=255)

                                 aGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAt                                                       >  1:5774770/1‑93 (MQ=255)

                                  gTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAtt                                                      <  1:1832732/93‑1 (MQ=255)

                                   tGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                     >  2:3791244/1‑93 (MQ=255)

                                   ggCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                     <  1:4853817/92‑1 (MQ=255)

                                      tGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTAc                                                  >  2:3879741/1‑93 (MQ=255)

                                       ggTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACt                                                 >  1:2499147/1‑93 (MQ=255)

                                        gTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTg                                                >  1:5265888/1‑93 (MQ=255)

                                         tGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGa                                               <  2:4251273/93‑1 (MQ=255)

                                          gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              >  2:5112880/1‑93 (MQ=255)

                                          gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              <  1:1035816/93‑1 (MQ=255)

                                          gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              <  1:3272090/93‑1 (MQ=255)

                                           aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAcc                                             >  1:7773/1‑92 (MQ=255)

                                           aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACg                                             >  1:3014552/1‑93 (MQ=255)

                                            ttGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGc                                            <  1:906828/93‑1 (MQ=255)

                                              gCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGccc                                          <  2:3082354/93‑1 (MQ=255)

                                              gCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGccc                                          <  1:4110917/93‑1 (MQ=255)

                                                tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgc                                        >  2:2805550/1‑93 (MQ=255)

                                                tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgc                                        <  2:648104/93‑1 (MQ=255)

                                                 gACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgct                                       >  1:1148072/1‑93 (MQ=255)

                                                  aCAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctg                                      >  1:140423/1‑93 (MQ=255)

                                                   cAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgc                                     >  1:2281453/1‑93 (MQ=255)

                                                   cAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgc                                     <  2:2949199/93‑1 (MQ=255)

                                                    aGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgct                                    >  1:4291301/1‑93 (MQ=255)

                                                     gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGctgctg                                   >  2:2276519/1‑93 (MQ=255)

                                                     gcggcgCTACGCCGGATTTAGTGGCTTCTGATTTGGCCTCTCAGGCTGAACACGGCCCGGACTCACAGGCGATTTTACTGACGCCcgccgccg                                   >  2:5315778/1‑91 (MQ=255)

                                                      cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                  <  1:3336357/93‑1 (MQ=255)

                                                      cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                  <  1:3711425/93‑1 (MQ=255)

                                                      cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                  <  1:3560894/93‑1 (MQ=255)

                                                        gcgcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGata                                <  1:3458968/93‑1 (MQ=255)

                                                         cgcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGatat                               <  1:1681641/93‑1 (MQ=255)

                                                          gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              >  1:5219739/1‑93 (MQ=255)

                                                          gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              >  2:2185002/1‑93 (MQ=255)

                                                          gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              <  1:1575538/93‑1 (MQ=255)

                                                           cTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATgg                             <  1:3360474/93‑1 (MQ=255)

                                                            tACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgc                            <  1:2599152/93‑1 (MQ=255)

                                                             aCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgcg                           <  1:5494341/93‑1 (MQ=255)

                                                             aCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCCGATATGgcg                           >  2:3551516/1‑93 (MQ=255)

                                                                ccGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgt                        <  2:3612438/93‑1 (MQ=255)

                                                                ccGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgt                        <  1:3205664/93‑1 (MQ=255)

                                                                 cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                       <  2:2593999/93‑1 (MQ=255)

                                                                 cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                       >  2:5635449/1‑93 (MQ=255)

                                                                 cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                       >  2:4733538/1‑93 (MQ=255)

                                                                  ggATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcg                      <  1:3194597/93‑1 (MQ=255)

                                                                   gATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgc                     >  2:3441416/1‑93 (MQ=255)

                                                                   gATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgc                     >  1:192424/1‑93 (MQ=255)

                                                                   gATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGGGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgc                     >  1:182010/1‑93 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTTACGCCCGCTGCTGATATGGcgcgtcgcgt                   >  1:2451643/1‑93 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   >  2:1117382/1‑93 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   <  1:2961038/93‑1 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   >  1:3310648/1‑93 (MQ=255)

                                                                      ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                  <  1:3547789/93‑1 (MQ=255)

                                                                      ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                  <  1:5305848/93‑1 (MQ=255)

                                                                        cGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGc                <  2:2711543/93‑1 (MQ=255)

                                                                          tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg              >  2:3832717/1‑93 (MQ=255)

                                                                          tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg              >  2:4175570/1‑93 (MQ=255)

                                                                           ggCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGa             >  1:4398471/1‑93 (MQ=255)

                                                                           ggCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGa             <  2:2975722/93‑1 (MQ=255)

                                                                              ttCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGc          >  1:3627908/1‑93 (MQ=255)

                                                                               tCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGcc         <  1:5051158/93‑1 (MQ=255)

                                                                                cTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCg        <  1:1455327/93‑1 (MQ=255)

                                                                                 tGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGt       <  2:2150389/93‑1 (MQ=255)

                                                                                   aTTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCg     <  1:4611957/93‑1 (MQ=255)

                                                                                    tttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGa    >  1:3332576/1‑93 (MQ=255)

                                                                                    tttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGa    >  1:1216910/1‑93 (MQ=255)

                                                                                     ttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa   <  2:1451777/93‑1 (MQ=255)

                                                                                      tGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAc  >  1:4722283/1‑93 (MQ=255)

                                                                                      tGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAc  <  2:4414733/93‑1 (MQ=255)

TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC  >  NC_000913/2089799‑2089977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCA  >  NC_000913/2089796‑2089981

CGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCC                                                                                              <  SRR4302533.1986938/94‑1 (MQ=60)

 GGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCT                                                                                             <  SRR4302533.1331613/94‑1 (MQ=60)

   TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCT                                                                                           >  SRR4302533.5991737/1‑94 (MQ=60)

   TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCT                                                                                           <  SRR4302533.822041/94‑1 (MQ=60)

    GCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTC                                                                                          <  SRR4302533.5578583/94‑1 (MQ=60)

     CGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCA                                                                                         <  SRR4302533.3105018/94‑1 (MQ=60)

      GGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAG                                                                                        <  SRR4302533.3578887/94‑1 (MQ=60)

        CGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGC                                                                                      >  SRR4302533.4273284/1‑94 (MQ=60)

        CGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGC                                                                                      >  SRR4302533.5664897/1‑94 (MQ=60)

         GATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCT                                                                                     >  SRR4302533.3093482/1‑94 (MQ=60)

         GATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCT                                                                                     <  SRR4302533.2400165/94‑1 (MQ=60)

         GATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCT                                                                                     >  SRR4302533.1207216/1‑94 (MQ=60)

         GATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCT                                                                                     >  SRR4302533.888963/1‑94 (MQ=60)

            CGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAA                                                                                  >  SRR4302533.4015400/1‑94 (MQ=60)

             GATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAAC                                                                                 >  SRR4302533.430888/1‑94 (MQ=60)

               TATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACAC                                                                               <  SRR4302533.1337434/94‑1 (MQ=60)

                ATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACG                                                                              >  SRR4302533.1791948/1‑94 (MQ=60)

                 TGCCCGCAGGCCCGTCGGAAGTGCTGGTAATTGCTGACAGCGGCGCTAAGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGG                                                                             <  SRR4302533.6342986/94‑1 (MQ=60)

                  GCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGC                                                                            <  SRR4302533.19871/94‑1 (MQ=60)

                   CCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCC                                                                           <  SRR4302533.1450912/94‑1 (MQ=60)

                   CCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCC                                                                           >  SRR4302533.2743639/1‑94 (MQ=60)

                     CGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCG                                                                         >  SRR4302533.3357665/1‑94 (MQ=60)

                      GCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGG                                                                        <  SRR4302533.4048763/94‑1 (MQ=60)

                       CAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACCCGGCCCGGA                                                                       >  SRR4302533.2068331/1‑94 (MQ=60)

                        AGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGAC                                                                      <  SRR4302533.2549704/94‑1 (MQ=60)

                           CCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCA                                                                   >  SRR4302533.1276356/1‑94 (MQ=60)

                            CCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCAC                                                                  <  SRR4302533.6259641/94‑1 (MQ=60)

                            CCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCAC                                                                  >  SRR4302533.6283529/1‑94 (MQ=60)

                             CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACA                                                                 <  SRR4302533.4989392/94‑1 (MQ=60)

                             CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACA                                                                 >  SRR4302533.3312911/1‑94 (MQ=60)

                             CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACA                                                                 >  SRR4302533.734690/1‑94 (MQ=60)

                               TCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGG                                                               <  SRR4302533.126317/94‑1 (MQ=60)

                               GCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGG                                                               <  SRR4302533.1778431/94‑1 (MQ=60)

                                CGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGT                                                              <  SRR4302533.5261184/94‑1 (MQ=60)

                                 GGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTG                                                             >  SRR4302533.2495158/1‑94 (MQ=60)

                                 GGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTG                                                             >  SRR4302533.5376615/1‑94 (MQ=60)

                                   AAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAT                                                           >  SRR4302533.5809643/1‑94 (MQ=60)

                                    AGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGGCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATT                                                          <  SRR4302533.2910838/94‑1 (MQ=60)

                                     GTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTT                                                         <  SRR4302533.1843918/94‑1 (MQ=60)

                                      GGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTT                                                        <  SRR4302533.4883233/94‑1 (MQ=60)

                                       GCTTGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTA                                                       <  SRR4302533.2619191/94‑1 (MQ=60)

                                        CTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTAC                                                      <  SRR4302533.5175535/94‑1 (MQ=60)

                                         TGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACT                                                     >  SRR4302533.2514564/1‑94 (MQ=60)

                                          GGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTG                                                    >  SRR4302533.5297488/1‑94 (MQ=60)

                                             GATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACG                                                 <  SRR4302533.3292281/94‑1 (MQ=60)

                                             GATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACG                                                 >  SRR4302533.3032988/1‑94 (MQ=60)

                                             GATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACG                                                 <  SRR4302533.1041905/94‑1 (MQ=60)

                                             GATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACC                                                 >  SRR4302533.7839/1‑94 (MQ=60)

                                               TTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCC                                               <  SRR4302533.168086/94‑1 (MQ=60)

                                               TTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCC                                               <  SRR4302533.912144/94‑1 (MQ=60)

                                                TGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCC                                              <  SRR4302533.4370900/94‑1 (MQ=60)

                                                 GCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCG                                             <  SRR4302533.4135903/94‑1 (MQ=60)

                                                   TGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCT                                           >  SRR4302533.1154927/1‑94 (MQ=60)

                                                    GACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTG                                          >  SRR4302533.141317/1‑94 (MQ=60)

                                                     ACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGC                                         >  SRR4302533.2295577/1‑94 (MQ=60)

                                                      CAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCT                                        >  SRR4302533.1915317/1‑94 (MQ=60)

                                                      CAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCT                                        >  SRR4302533.4317107/1‑94 (MQ=60)

                                                      CAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCT                                        <  SRR4302533.3476143/94‑1 (MQ=60)

                                                         CGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGAT                                     <  SRR4302533.3734216/94‑1 (MQ=60)

                                                         CGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGAT                                     <  SRR4302533.3582832/94‑1 (MQ=60)

                                                         CGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGAT                                     <  SRR4302533.3356973/94‑1 (MQ=60)

                                                         CGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGAT                                     >  SRR4302533.1564960/1‑94 (MQ=60)

                                                           GCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATAT                                   <  SRR4302533.3480322/94‑1 (MQ=60)

                                                            CGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATG                                  >  SRR4302533.5251155/1‑94 (MQ=60)

                                                            CGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATG                                  <  SRR4302533.1691826/94‑1 (MQ=60)

                                                            CGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATG                                  >  SRR4302533.3348503/1‑94 (MQ=60)

                                                             GCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGG                                 <  SRR4302533.1585024/94‑1 (MQ=60)

                                                             GCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGG                                 <  SRR4302533.1723214/94‑1 (MQ=60)

                                                              CTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGC                                <  SRR4302533.2230986/94‑1 (MQ=60)

                                                              CTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGC                                >  SRR4302533.6214975/1‑94 (MQ=60)

                                                              CTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGC                                <  SRR4302533.3381236/94‑1 (MQ=60)

                                                               TACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCG                               >  SRR4302533.65743/1‑94 (MQ=60)

                                                               TACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCG                               <  SRR4302533.2615127/94‑1 (MQ=60)

                                                                ACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGC                              <  SRR4302533.5527486/94‑1 (MQ=60)

                                                                 CGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCG                             >  SRR4302533.3220320/1‑94 (MQ=60)

                                                                  GCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGT                            >  SRR4302533.4629575/1‑94 (MQ=60)

                                                                   CCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTC                           <  SRR4302533.5976722/94‑1 (MQ=60)

                                                                   CCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTC                           <  SRR4302533.3225400/94‑1 (MQ=60)

                                                                     GGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGC                         <  SRR4302533.3214252/94‑1 (MQ=60)

                                                                     GGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGC                         <  SRR4302533.3099984/94‑1 (MQ=60)

                                                                     GGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGC                         >  SRR4302533.193636/1‑94 (MQ=60)

                                                                     GGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGGGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGC                         >  SRR4302533.183141/1‑94 (MQ=60)

                                                                       ATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTTACGCCCGCTGCTGATATGGCGCGTCGCGT                       >  SRR4302533.2466777/1‑94 (MQ=60)

                                                                       ATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGT                       >  SRR4302533.3331090/1‑94 (MQ=60)

                                                                       ATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGT                       >  SRR4302533.4666109/1‑94 (MQ=60)

                                                                        TTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTT                      <  SRR4302533.2979140/94‑1 (MQ=60)

                                                                         TTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTG                     <  SRR4302533.3569654/94‑1 (MQ=60)

                                                                         TTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTG                     <  SRR4302533.5337644/94‑1 (MQ=60)

                                                                           CGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCC                   <  SRR4302533.3959549/94‑1 (MQ=60)

                                                                           CGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCC                   >  SRR4302533.30742/1‑94 (MQ=60)

                                                                             TGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGA                 >  SRR4302533.4425026/1‑94 (MQ=60)

                                                                              GGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAG                <  SRR4302533.2762210/94‑1 (MQ=60)

                                                                               GCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGG               <  SRR4302533.3636290/94‑1 (MQ=60)

                                                                                CTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGCCGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGCGGC              >  SRR4302533.775540/1‑94 (MQ=60)

                                                                                CTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGC              >  SRR4302533.3650231/1‑94 (MQ=60)

                                                                                CTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGC              <  SRR4302533.2986796/94‑1 (MQ=60)

                                                                                CTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCCGACTCACAGGTGATTTTACTGACGTCCGCTGCTGATATGGCGCGTCGCGTTGCCGATGC              >  SRR4302533.2045182/1‑94 (MQ=60)

                                                                                 TTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTATTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCC             <  SRR4302533.3253343/94‑1 (MQ=60)

                                                                                 TTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCC             <  SRR4302533.3500371/94‑1 (MQ=60)

                                                                                  TCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCG            <  SRR4302533.5081703/94‑1 (MQ=60)

                                                                                   CTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGT           <  SRR4302533.1464100/94‑1 (MQ=60)

                                                                                    TGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTC          <  SRR4302533.5728085/94‑1 (MQ=60)

                                                                                     GATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCG         <  SRR4302533.4785642/94‑1 (MQ=60)

                                                                                      ATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGA        >  SRR4302533.3353169/1‑94 (MQ=60)

                                                                                      ATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGA        <  SRR4302533.4639900/94‑1 (MQ=60)

                                                                                      ATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGA        >  SRR4302533.1224219/1‑94 (MQ=60)

                                                                                        TTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC      <  SRR4302533.371718/94‑1 (MQ=60)

                                                                                        TTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC      >  SRR4302533.4750914/1‑94 (MQ=60)

                                                                                         TGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACG     <  SRR4302533.3128121/94‑1 (MQ=60)

                                                                                          GCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGC    <  SRR4302533.3681646/94‑1 (MQ=60)

                                                                                           CCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCC   >  SRR4302533.1668453/1‑94 (MQ=60)

                                                                                           CCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCC   >  SRR4302533.1960100/1‑94 (MQ=60)

                                                                                            CCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCA  <  SRR4302533.4347911/94‑1 (MQ=60)

CGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCA  >  NC_000913/2089796‑2089981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`