Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,375,871 T→C N470S (AAC→AGC)  menD ← 2‑oxoglutarate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,8710TC100.0% 657.4 / NA 206N470S (AAC→AGC) menD2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/108);  total (98/108)

Only 100 of 231 total aligned reads displayed.

GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960
                                                                                          |                                                                                         
gcgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctg                                                                                         >  1:5725101/1‑93 (MQ=255)
 cgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgt                                                                                        <  1:4229523/93‑1 (MQ=255)
 cgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgt                                                                                        <  2:3754751/93‑1 (MQ=255)
 cgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCAAGAAAATTTGCCCGCCgctgt                                                                                        <  1:4363259/93‑1 (MQ=255)
    gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgctgttgt                                                                                     >  2:3908011/1‑93 (MQ=255)
      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   >  2:4720171/1‑93 (MQ=255)
      tCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTc                                                                                   >  1:3050052/1‑93 (MQ=255)
       cAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTca                                                                                  <  2:4978130/93‑1 (MQ=255)
        aaaaTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcac                                                                                 <  1:5504631/93‑1 (MQ=255)
           aTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGGCCGCAGCTGTTGTTaaccaa                                                                              >  2:4165089/1‑92 (MQ=255)
           aTGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTcaccac                                                                              >  1:4564880/1‑93 (MQ=255)
             ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACaa                                                                            >  1:5859950/1‑93 (MQ=255)
              gACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAt                                                                           >  1:3987549/1‑93 (MQ=255)
                cGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTa                                                                         >  2:6274902/1‑93 (MQ=255)
                 cttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTaa                                                                        <  1:5282835/92‑1 (MQ=255)
                  ttttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTACCCGCCGCTGTTGTTCACCACAATTaac                                                                       >  1:6060629/1‑92 (MQ=255)
                     tGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAcc                                                                    >  1:256528/1‑93 (MQ=255)
                      gCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCa                                                                   <  1:3657888/93‑1 (MQ=255)
                        ggCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgc                                                                 <  1:1950238/93‑1 (MQ=255)
                          cATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcgg                                                               >  1:2499415/1‑93 (MQ=255)
                           aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggc                                                              <  1:3206099/93‑1 (MQ=255)
                           aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggc                                                              >  2:1045817/1‑93 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAg                                                          >  2:364450/1‑93 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAg                                                          >  1:5143855/1‑93 (MQ=255)
                               gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAg                                                          >  1:4483371/1‑93 (MQ=255)
                                aTAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGa                                                         <  2:3210669/93‑1 (MQ=255)
                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaa                                                        <  1:4106576/93‑1 (MQ=255)
                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaa                                                        >  1:1011086/1‑93 (MQ=255)
                                 tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaa                                                        >  2:5605811/1‑93 (MQ=255)
                                  aGAACCGCTCACGCTCGCTTGGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaaa                                                       <  2:5319053/93‑1 (MQ=255)
                                   gAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAc                                                      <  1:2976315/93‑1 (MQ=255)
                                     aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCt                                                    >  1:2702042/1‑93 (MQ=255)
                                       cgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGa                                                  <  2:790419/93‑1 (MQ=255)
                                        gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAACTGAc                                                 >  1:331192/1‑93 (MQ=255)
                                         ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCTCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACg                                                >  1:4089124/1‑93 (MQ=255)
                                         ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACAATTAACACCAGCGGCGCAGAAACCTGACg                                                >  2:4371184/1‑93 (MQ=255)
                                         ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACg                                                <  2:1561381/93‑1 (MQ=255)
                                          tcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGc                                               >  1:6219156/1‑93 (MQ=255)
                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATaa                                          >  2:2474489/1‑93 (MQ=255)
                                               ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATaa                                          <  2:693408/93‑1 (MQ=255)
                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  1:3622118/1‑93 (MQ=255)
                                                tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAc                                         >  2:2068449/1‑93 (MQ=255)
                                                 cgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACg                                        <  1:5636270/93‑1 (MQ=255)
                                                  gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAACGGCGCAGAAAACTGACGCCATAAAGc                                       >  1:4302427/1‑93 (MQ=255)
                                                   ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACgtc                                      >  1:1862351/1‑91 (MQ=255)
                                                   ctTTGCGGCGTTGGCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                      <  1:2761574/93‑1 (MQ=255)
                                                    tttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAAGCCa                                     >  1:1315705/1‑93 (MQ=255)
                                                    ttggcggcgTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCa                                     <  2:4481802/90‑1 (MQ=255)
                                                     ttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                    <  2:3062212/93‑1 (MQ=255)
                                                     ttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                    <  2:2533369/93‑1 (MQ=255)
                                                     ttGCGCCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                    <  2:1838466/93‑1 (MQ=255)
                                                         ggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCCCAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGcccgcccg                                >  2:4356700/1‑93 (MQ=255)
                                                          gcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt                               >  1:140657/1‑93 (MQ=255)
                                                           cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                              <  1:5807051/93‑1 (MQ=255)
                                                            gTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACCAATAAAACCCACGGGGCCCAAAACCGACGGAAAAAAGCCAGCGCGGTg                             >  2:4324129/1‑93 (MQ=255)
                                                             ttGGCAACAGCGAGAGAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                            >  1:5117073/1‑93 (MQ=255)
                                                             ttGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                            <  1:4765727/93‑1 (MQ=255)
                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          >  2:5835787/1‑93 (MQ=255)
                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          <  1:2928968/93‑1 (MQ=255)
                                                               ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          <  1:5380796/93‑1 (MQ=255)
                                                               ggCAAAAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                          <  2:6001411/93‑1 (MQ=255)
                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                         >  2:4434526/1‑93 (MQ=255)
                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                         <  2:4557446/93‑1 (MQ=255)
                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                         >  1:2083068/1‑93 (MQ=255)
                                                                gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGAGTTGAGAt                         <  2:6149132/93‑1 (MQ=255)
                                                                 cAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAta                        >  2:4659298/1‑92 (MQ=255)
                                                                   aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                      <  2:1759570/93‑1 (MQ=255)
                                                                    cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                     <  1:1645295/93‑1 (MQ=255)
                                                                    cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                     <  1:4125707/93‑1 (MQ=255)
                                                                    cAGAGAGAAACTTGCCCCGCCGCTTTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                     <  2:2188233/93‑1 (MQ=255)
                                                                     aGCGAGAAAATTTGCCCGGCCCGGTTGTTTACCACAATTAACACCAGCGGCGCAGAAAACTGACGCAATAAAGGCACGGCGTTGAGAGCGGaa                    >  2:2314683/1‑93 (MQ=255)
                                                                      gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                   <  1:4151838/93‑1 (MQ=255)
                                                                       gcagaAAATTGGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  <  1:3114301/91‑1 (MQ=255)
                                                                       cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  >  2:3848950/1‑93 (MQ=255)
                                                                       cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                  <  2:5676642/93‑1 (MQ=255)
                                                                        gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                 <  1:4611565/93‑1 (MQ=255)
                                                                        gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                 <  2:6104955/93‑1 (MQ=255)
                                                                        gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                 <  2:2164739/93‑1 (MQ=255)
                                                                        gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGGTGAGATCGTAAAGt                 >  2:3538544/1‑93 (MQ=255)
                                                                         agaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTg                >  2:5090176/1‑93 (MQ=255)
                                                                         agaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTg                >  2:1444184/1‑93 (MQ=255)
                                                                          gaAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc               <  2:2882622/93‑1 (MQ=255)
                                                                          aaaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc               <  2:5799185/92‑1 (MQ=255)
                                                                           aaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCg              <  2:3994520/93‑1 (MQ=255)
                                                                           aaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCg              >  1:5502529/1‑93 (MQ=255)
                                                                            aaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg             <  1:5024795/93‑1 (MQ=255)
                                                                             aaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGga            >  2:2005946/1‑93 (MQ=255)
                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga          <  2:6211062/93‑1 (MQ=255)
                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAACGCGTTGAGATCGTAAAGTGCGgagc          >  1:659377/1‑92 (MQ=255)
                                                                               tttGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAACTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagg          >  1:5818657/1‑92 (MQ=255)
                                                                                ttGCCCGTCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagag         >  2:5986631/1‑93 (MQ=255)
                                                                                ttGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagag         <  2:2632119/93‑1 (MQ=255)
                                                                                 tGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga        <  2:4104507/93‑1 (MQ=255)
                                                                                 tGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga        >  1:5236152/1‑93 (MQ=255)
                                                                                   cccGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc      >  1:3563773/1‑93 (MQ=255)
                                                                                   cccGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc      <  1:2630707/93‑1 (MQ=255)
                                                                                     cgccgcTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGc    <  2:1891718/93‑1 (MQ=255)
                                                                                      gccgCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGcc   <  2:2392242/93‑1 (MQ=255)
                                                                                       ccgCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc  >  2:2814550/1‑93 (MQ=255)
                                                                                       ccgCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc  <  2:765485/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCC  >  NC_000913/2375781‑2375960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCAC  >  NC_000913/2375778‑2375962
                                                                                             |                                                                                           
GCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACTCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGC                                                                                             <  SRR4302533.4921156/94‑1 (MQ=60)
 CGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCT                                                                                            <  SRR4302533.2562090/94‑1 (MQ=60)
  GGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTG                                                                                           >  SRR4302533.5759665/1‑94 (MQ=60)
  GGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTG                                                                                           >  SRR4302533.4211427/1‑94 (MQ=60)
    CGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTT                                                                                         >  SRR4302533.5771220/1‑94 (MQ=60)
    CGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTT                                                                                         <  SRR4302533.4255092/94‑1 (MQ=60)
    CGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCAAGAAAATTTGCCCGCCGCTGTT                                                                                         <  SRR4302533.4389597/94‑1 (MQ=60)
     GTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTG                                                                                        >  SRR4302533.4099636/1‑94 (MQ=60)
       GCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTT                                                                                      >  SRR4302533.4821328/1‑94 (MQ=60)
       GCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTT                                                                                      >  SRR4302533.3243316/1‑94 (MQ=60)
        CTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTC                                                                                     >  SRR4302533.2062634/1‑94 (MQ=60)
        CTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTC                                                                                     >  SRR4302533.3068719/1‑94 (MQ=60)
           AAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACC                                                                                  <  SRR4302533.5537845/94‑1 (MQ=60)
            AAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCA                                                                                 <  SRR4302533.5536637/94‑1 (MQ=60)
            AAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCA                                                                                 >  SRR4302533.4122982/1‑94 (MQ=60)
             AATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCAC                                                                                >  SRR4302533.4592556/1‑94 (MQ=60)
               TGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAA                                                                              >  SRR4302533.332789/1‑94 (MQ=60)
               TGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAA                                                                              >  SRR4302533.5895376/1‑94 (MQ=60)
                GGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAAT                                                                             >  SRR4302533.4011839/1‑94 (MQ=60)
                 GACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATT                                                                            <  SRR4302533.5850896/94‑1 (MQ=60)
                   CGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAA                                                                          <  SRR4302533.137699/94‑1 (MQ=60)
                   CGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTGGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAA                                                                          <  SRR4302533.1171067/94‑1 (MQ=60)
                    GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTACCCGCCGCTGTTGTTCACCACAATTAAC                                                                         >  SRR4302533.6097319/1‑94 (MQ=60)
                    CTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAC                                                                         <  SRR4302533.5314482/94‑1 (MQ=60)
                      TTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAC                                                                       <  SRR4302533.5280630/94‑1 (MQ=60)
                       TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACC                                                                      >  SRR4302533.258093/1‑94 (MQ=60)
                        TGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCA                                                                     <  SRR4302533.2735769/94‑1 (MQ=60)
                        TGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCA                                                                     <  SRR4302533.5890508/94‑1 (MQ=60)
                        TGCGACATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCA                                                                     <  SRR4302533.5393088/94‑1 (MQ=60)
                         GCGGCATCAGATAGAAACGCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAG                                                                    <  SRR4302533.4606839/94‑1 (MQ=60)
                         GCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAG                                                                    <  SRR4302533.3680380/94‑1 (MQ=60)
                         GCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAG                                                                    <  SRR4302533.3680379/94‑1 (MQ=60)
                          CGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGC                                                                   <  SRR4302533.4211501/94‑1 (MQ=60)
                           GGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCG                                                                  <  SRR4302533.1962124/94‑1 (MQ=60)
                            GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGG                                                                 >  SRR4302533.2979672/1‑94 (MQ=60)
                            GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGG                                                                 >  SRR4302533.4137132/1‑94 (MQ=60)
                            GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGG                                                                 >  SRR4302533.2514832/1‑94 (MQ=60)
                             CATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGC                                                                >  SRR4302533.2597472/1‑94 (MQ=60)
                              ATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCG                                                               <  SRR4302533.3225839/94‑1 (MQ=60)
                              ATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCG                                                               >  SRR4302533.187991/1‑94 (MQ=60)
                                CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCA                                                             <  SRR4302533.5238223/94‑1 (MQ=60)
                                 AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACAATTAACACCAGCGGCGCAG                                                            >  SRR4302533.2662398/1‑94 (MQ=60)
                                 AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAG                                                            >  SRR4302533.4510519/1‑94 (MQ=60)
                                 AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAG                                                            >  SRR4302533.872714/1‑94 (MQ=60)
                                 AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAG                                                            >  SRR4302533.5174942/1‑94 (MQ=60)
                                  GATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGA                                                           <  SRR4302533.4289061/94‑1 (MQ=60)
                                  GATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGA                                                           >  SRR4302533.6119545/1‑94 (MQ=60)
                                   ATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAA                                                          >  SRR4302533.1017002/1‑94 (MQ=60)
                                    TAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAA                                                         >  SRR4302533.776614/1‑94 (MQ=60)
                                    TAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAA                                                         <  SRR4302533.4131548/94‑1 (MQ=60)
                                      GAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACC                                                       <  SRR4302533.2994526/94‑1 (MQ=60)
                                       AAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCT                                                      >  SRR4302533.2718617/1‑94 (MQ=60)
                                       AAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCT                                                      <  SRR4302533.2276468/94‑1 (MQ=60)
                                        AACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTG                                                     <  SRR4302533.5165512/94‑1 (MQ=60)
                                          CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAC                                                   >  SRR4302533.3021115/1‑94 (MQ=60)
                                          CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAC                                                   >  SRR4302533.3186494/1‑94 (MQ=60)
                                          CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAACTGAC                                                   >  SRR4302533.333220/1‑94 (MQ=60)
                                           GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCTCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACG                                                  >  SRR4302533.4114019/1‑94 (MQ=60)
                                           GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACG                                                  <  SRR4302533.5693900/94‑1 (MQ=60)
                                           CCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACG                                                  <  SRR4302533.4576601/94‑1 (MQ=60)
                                            CTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGC                                                 >  SRR4302533.6256442/1‑94 (MQ=60)
                                              CACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAA                                               >  SRR4302533.451790/1‑94 (MQ=60)
                                              CACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAA                                               >  SRR4302533.3668311/1‑94 (MQ=60)
                                                  CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAC                                           >  SRR4302533.3644407/1‑94 (MQ=60)
                                                   TCGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACG                                          <  SRR4302533.5521699/94‑1 (MQ=60)
                                                    CGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGC                                         <  SRR4302533.5670353/94‑1 (MQ=60)
                                                    CGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAACGGCGCAGAAAACTGACGCCATAAAGC                                         >  SRR4302533.4328310/1‑94 (MQ=60)
                                                     GCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGTC                                        >  SRR4302533.1873707/1‑94 (MQ=60)
                                                     GCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCC                                        >  SRR4302533.3089368/1‑94 (MQ=60)
                                                      CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                       >  SRR4302533.2533207/1‑94 (MQ=60)
                                                      CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAAGCCA                                       >  SRR4302533.1323598/1‑94 (MQ=60)
                                                      CTTTGCGGCGTTGGCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                       <  SRR4302533.2778501/94‑1 (MQ=60)
                                                         TGCGGCGTTGGCAACAGCGATAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCG                                    <  SRR4302533.4944606/94‑1 (MQ=60)
                                                          GCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGC                                   >  SRR4302533.3416073/1‑94 (MQ=60)
                                                           CGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCG                                  <  SRR4302533.6262369/94‑1 (MQ=60)
                                                           CGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCCGAAGCAATCACGCCAGCGCG                                  >  SRR4302533.4741605/1‑94 (MQ=60)
                                                           CGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCG                                  <  SRR4302533.5565897/94‑1 (MQ=60)
                                                            GGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGT                                 >  SRR4302533.141555/1‑94 (MQ=60)
                                                              CGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTG                               <  SRR4302533.5842116/94‑1 (MQ=60)
                                                              CGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACCCCAGCGCGTTG                               >  SRR4302533.5246071/1‑94 (MQ=60)
                                                               GTTGGCAACAGCGAGAGAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                              >  SRR4302533.5147996/1‑94 (MQ=60)
                                                               GTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                              >  SRR4302533.550876/1‑94 (MQ=60)
                                                                TTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAG                             <  SRR4302533.4794625/94‑1 (MQ=60)
                                                                GTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAG                             <  SRR4302533.3841533/94‑1 (MQ=60)
                                                                  GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                           <  SRR4302533.1725112/94‑1 (MQ=60)
                                                                  GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                           <  SRR4302533.5413124/94‑1 (MQ=60)
                                                                  GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                           >  SRR4302533.2095891/1‑94 (MQ=60)
                                                                  GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                           <  SRR4302533.2946886/94‑1 (MQ=60)
                                                                     AACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGT                        >  SRR4302533.2170882/1‑94 (MQ=60)
                                                                     AACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGT                        <  SRR4302533.2901365/94‑1 (MQ=60)
                                                                       CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                      <  SRR4302533.4150752/94‑1 (MQ=60)
                                                                       CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                      <  SRR4302533.1655257/94‑1 (MQ=60)
                                                                       CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                      <  SRR4302533.1835545/94‑1 (MQ=60)
                                                                       AAGCGAAAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCGATAACGCCAGCGCGTTGAGATCGTAA                      <  SRR4302533.4509171/94‑1 (MQ=60)
                                                                        aggaAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAA                     <  SRR4302533.4364640/90‑1 (MQ=60)
                                                                         GCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAG                    <  SRR4302533.4176994/94‑1 (MQ=60)
                                                                          gcAGAAAATTGGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGT                   <  SRR4302533.3133453/92‑1 (MQ=60)
                                                                          gaaaAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGT                   <  SRR4302533.3620627/90‑1 (MQ=60)
                                                                           GAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTG                  <  SRR4302533.4639505/94‑1 (MQ=60)
                                                                            AGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGC                 >  SRR4302533.5672845/1‑94 (MQ=60)
                                                                             GAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCG                <  SRR4302533.2972983/94‑1 (MQ=60)
                                                                             GAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCG                >  SRR4302533.5535729/1‑94 (MQ=60)
                                                                               AAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGA              <  SRR4302533.5055221/94‑1 (MQ=60)
                                                                                 ATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAACGCGTTGAGATCGTAAAGTGCGGAGC            >  SRR4302533.663262/1‑94 (MQ=60)
                                                                                 ATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAACTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGG            >  SRR4302533.5853805/1‑94 (MQ=60)
                                                                                  TTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAGGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAG           <  SRR4302533.5096170/94‑1 (MQ=60)
                                                                                   TTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGA          >  SRR4302533.5267634/1‑94 (MQ=60)
                                                                                    TGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAT         <  SRR4302533.1146373/94‑1 (MQ=60)
                                                                                    TGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAT         <  SRR4302533.2703147/94‑1 (MQ=60)
                                                                                     GCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC        <  SRR4302533.5978344/94‑1 (MQ=60)
                                                                                     GCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC        >  SRR4302533.5982236/1‑94 (MQ=60)
                                                                                     GCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC        >  SRR4302533.3585732/1‑94 (MQ=60)
                                                                                      CCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG       <  SRR4302533.2646872/94‑1 (MQ=60)
                                                                                      CCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG       <  SRR4302533.4127687/94‑1 (MQ=60)
                                                                                      CCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG       >  SRR4302533.1526708/1‑94 (MQ=60)
                                                                                       CCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGC      >  SRR4302533.4174405/1‑94 (MQ=60)
                                                                                          CCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCA   >  SRR4302533.3851969/1‑94 (MQ=60)
                                                                                           CGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCAC  >  SRR4302533.233220/1‑94 (MQ=60)
                                                                                             |                                                                                           
GCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCAC  >  NC_000913/2375778‑2375962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑