Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,403,767	G→A	P299S (CCG→TCG)	lrhA ←	NADH dehydrogenase transcriptional regulator, LysR family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,403,767	0	G	A	99.5%	794.8 / NA	215	P299S (CCG→TCG)	lrhA	NADH dehydrogenase transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (120/94); minor base T (1/0); total (121/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

CTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGAT  >  NC_000913/2403677‑2403856

cTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAg                                                                                         >  2:1812144/1‑93 (MQ=255)

 tCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGc                                                                                        >  1:4828974/1‑93 (MQ=255)

   gTTTTTTACACTATTGTCTCAGGAATTATCTATTGTCCGACGATTACTCGATATTCCTTTCAATCAACAATGAATCATCCCCTTCCGAATCAg                                                                                      >  2:1156914/1‑93 (MQ=255)

   gTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAg                                                                                      <  2:3090488/93‑1 (MQ=255)

   gTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAg                                                                                      >  1:4277138/1‑93 (MQ=255)

   gTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATACCCTTCCGAAGCAg                                                                                      >  1:65944/1‑93 (MQ=255)

      ttttACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACa                                                                                   <  2:2693477/93‑1 (MQ=255)

       tttACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACAt                                                                                  >  1:4387555/1‑93 (MQ=255)

        ttACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACAtt                                                                                 >  1:1375349/1‑93 (MQ=255)

        ttACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACAtt                                                                                 <  2:2221031/93‑1 (MQ=255)

         tACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTg                                                                                >  1:5693468/1‑93 (MQ=255)

         tACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTg                                                                                <  1:3395578/93‑1 (MQ=255)

           cacTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTggg                                                                              >  1:180285/1‑93 (MQ=255)

            acTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGc                                                                             <  1:998658/93‑1 (MQ=255)

            acTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGc                                                                             <  2:5621491/93‑1 (MQ=255)

             cTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCt                                                                            >  2:4371178/1‑93 (MQ=255)

               aTTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGt                                                                          <  1:3622149/93‑1 (MQ=255)

                ttGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTa                                                                         >  2:2339100/1‑93 (MQ=255)

                 tGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTAt                                                                        <  1:3550463/93‑1 (MQ=255)

                   tctcAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCAGAAGCAGACATTGGGCTGTATTg                                                                      >  2:2078363/1‑93 (MQ=255)

                    ctcAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGc                                                                     <  2:6279926/93‑1 (MQ=255)

                          aaTTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGa                                                               <  2:4068808/93‑1 (MQ=255)

                          aaTTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGa                                                               <  2:4917568/93‑1 (MQ=255)

                           aTTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGAt                                                              >  1:2525223/1‑93 (MQ=255)

                             tatctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTa                                                            <  1:3157779/93‑1 (MQ=255)

                             tatctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTa                                                            >  1:5134043/1‑93 (MQ=255)

                             tatctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTa                                                            <  1:2666066/93‑1 (MQ=255)

                               tctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATg                                                          <  1:6164298/93‑1 (MQ=255)

                               tctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATg                                                          <  2:3863890/93‑1 (MQ=255)

                               tctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATg                                                          >  2:1751470/1‑93 (MQ=255)

                                ctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATgg                                                         >  1:5402864/1‑93 (MQ=255)

                                 tatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGt                                                        >  1:1742558/1‑93 (MQ=255)

                                  atcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTa                                                       >  2:2984300/1‑93 (MQ=255)

                                  atcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTa                                                       <  1:4601589/93‑1 (MQ=255)

                                  atcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTa                                                       <  2:5681488/93‑1 (MQ=255)

                                   tcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAg                                                      <  1:6301171/93‑1 (MQ=255)

                                     gtccgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGct                                                    <  1:3067814/93‑1 (MQ=255)

                                      tccgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGctc                                                   >  1:4412949/1‑93 (MQ=255)

                                      tccgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGctc                                                   >  2:2049541/1‑93 (MQ=255)

                                       ccgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGctct                                                  >  1:3662972/1‑93 (MQ=255)

                                       ccgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGctct                                                  >  2:5762056/1‑93 (MQ=255)

                                        cgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCtctc                                                 >  2:4743751/1‑93 (MQ=255)

                                        cgtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCtctc                                                 >  1:1436572/1‑93 (MQ=255)

                                         gtcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTcc                                                >  1:1856634/1‑93 (MQ=255)

                                          tcGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCTGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCa                                               >  2:1877021/1‑93 (MQ=255)

                                           cGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCAt                                              >  2:5267931/1‑93 (MQ=255)

                                           cGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCAt                                              >  1:4939552/1‑93 (MQ=255)

                                            gATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATc                                             <  2:429431/93‑1 (MQ=255)

                                            gATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATc                                             >  2:1384287/1‑93 (MQ=255)

                                            gATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATc                                             >  2:2968586/1‑93 (MQ=255)

                                             aTTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCg                                            >  1:5567646/1‑93 (MQ=255)

                                             aTTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCg                                            >  2:5627310/1‑93 (MQ=255)

                                               tACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGcc                                          <  1:5819327/93‑1 (MQ=255)

                                               tACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGcc                                          <  2:1297776/93‑1 (MQ=255)

                                                 cTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTg                                        >  2:2890167/1‑93 (MQ=255)

                                                 cTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTg                                        >  1:1314584/1‑93 (MQ=255)

                                                  tCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGa                                       >  1:5035692/1‑93 (MQ=255)

                                                  tCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGa                                       <  1:4897584/93‑1 (MQ=255)

                                                  tCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGa                                       <  1:2344137/93‑1 (MQ=255)

                                                   cGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGAt                                      <  1:1072207/93‑1 (MQ=255)

                                                     atatCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAg                                    >  2:1336327/1‑93 (MQ=255)

                                                     atatCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAg                                    <  1:1242455/93‑1 (MQ=255)

                                                      tatCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGa                                   <  2:1225187/93‑1 (MQ=255)

                                                      tatCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGa                                   <  2:5948390/93‑1 (MQ=255)

                                                      tatCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGa                                   >  2:5841891/1‑93 (MQ=255)

                                                      tatCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGa                                   >  1:4111875/1‑93 (MQ=255)

                                                       atCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGAt                                  <  1:1702943/93‑1 (MQ=255)

                                                       atCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGAt                                  <  1:4462790/93‑1 (MQ=255)

                                                        tCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATc                                 >  2:4662820/1‑93 (MQ=255)

                                                         cccTTTCAATCAACAATGAATCATCCCCTTCCGTAGCAGACATTGGGCTGTATTGCCACGGCTTATGGTAGCTCTCCATCGGCTGATAGATCa                                >  2:1829928/1‑93 (MQ=255)

                                                         cccTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCa                                <  2:3756512/93‑1 (MQ=255)

                                                           cTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCAcc                              >  1:1126653/1‑93 (MQ=255)

                                                           cTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCAcc                              <  2:2792096/93‑1 (MQ=255)

                                                            tttCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCt                             >  2:3470512/1‑93 (MQ=255)

                                                             ttCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTg                            <  2:2422709/93‑1 (MQ=255)

                                                             ttCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTg                            <  2:1902687/93‑1 (MQ=255)

                                                             ttCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTg                            <  1:935571/93‑1 (MQ=255)

                                                             ttCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACATg                            >  2:3166274/1‑93 (MQ=255)

                                                               caatcaaCAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTggg                          >  2:6305403/1‑93 (MQ=255)

                                                                 atcaaCAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGcc                        <  2:5039387/93‑1 (MQ=255)

                                                                   caacaaTGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTTCCACGGATTATGGTAGCTATCCATAGCCTGATAGATTACCTGTGCCAg                      >  2:1659032/1‑93 (MQ=255)

                                                                        aTGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCat                 <  1:2510422/93‑1 (MQ=255)

                                                                            atcatcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCattatt             <  1:5174934/93‑1 (MQ=255)

                                                                            atcatcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCattatt             <  1:2248954/93‑1 (MQ=255)

                                                                             tcatcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCAttatta            >  1:387762/1‑93 (MQ=255)

                                                                             tcatcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCAttatta            >  1:308837/1‑93 (MQ=255)

                                                                               atcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACt          >  1:5075154/1‑93 (MQ=255)

                                                                               atcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACt          >  1:2111762/1‑93 (MQ=255)

                                                                                tcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTg         <  2:1446210/93‑1 (MQ=255)

                                                                                tcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTg         >  2:658295/1‑93 (MQ=255)

                                                                                tcCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTg         >  2:3719387/1‑93 (MQ=255)

                                                                                 ccccTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGTGCCAGCTCATTATTACTgg        >  1:4214956/1‑93 (MQ=255)

                                                                                 ccccTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTgg        >  2:3298547/1‑93 (MQ=255)

                                                                                  cccTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGa       >  1:2963854/1‑93 (MQ=255)

                                                                                  cccTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGa       >  2:3529334/1‑93 (MQ=255)

                                                                                   ccTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAc      <  2:2641507/93‑1 (MQ=255)

                                                                                   ccTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAc      >  1:2315040/1‑93 (MQ=255)

                                                                                    cTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACg     >  2:6176637/1‑93 (MQ=255)

                                                                                      tCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGa   <  1:2641069/93‑1 (MQ=255)

                                                                                       ccGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGAt  <  2:705650/93‑1 (MQ=255)

CTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGAT  >  NC_000913/2403677‑2403856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA  >  NC_000913/2403674‑2403860

CGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGA                                                                                               >  SRR4302533.2180536/1‑94 (MQ=60)

 GGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAA                                                                                              >  SRR4302533.2518199/1‑94 (MQ=60)

  GCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAG                                                                                             >  SRR4302533.2286575/1‑94 (MQ=60)

   CTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGC                                                                                            >  SRR4302533.4858238/1‑94 (MQ=60)

   CTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGC                                                                                            >  SRR4302533.2673040/1‑94 (MQ=60)

     CGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAG                                                                                          >  SRR4302533.4302860/1‑94 (MQ=60)

     CGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAG                                                                                          >  SRR4302533.6266580/1‑94 (MQ=60)

     CGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATACCCTTCCGAAGCAG                                                                                          >  SRR4302533.66370/1‑94 (MQ=60)

       TTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGAC                                                                                        >  SRR4302533.3112121/1‑94 (MQ=60)

         TTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACAT                                                                                      >  SRR4302533.4414044/1‑94 (MQ=60)

         TTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACAT                                                                                      <  SRR4302533.2717750/94‑1 (MQ=60)

          TTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATT                                                                                     >  SRR4302533.1383625/1‑94 (MQ=60)

           TTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTG                                                                                    >  SRR4302533.5727850/1‑94 (MQ=60)

            TACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGG                                                                                   <  SRR4302533.3416547/94‑1 (MQ=60)

             ACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGG                                                                                  >  SRR4302533.181409/1‑94 (MQ=60)

               ACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCT                                                                                <  SRR4302533.1004487/94‑1 (MQ=60)

                 TATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGT                                                                              <  SRR4302533.3104012/94‑1 (MQ=60)

                 TATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGT                                                                              >  SRR4302533.1512622/1‑94 (MQ=60)

                  ATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTA                                                                             <  SRR4302533.3644437/94‑1 (MQ=60)

                  ATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTA                                                                             <  SRR4302533.3644438/94‑1 (MQ=60)

                    TGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATT                                                                           <  SRR4302533.3572337/94‑1 (MQ=60)

                    TGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATT                                                                           <  SRR4302533.702813/94‑1 (MQ=60)

                     GTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTG                                                                          <  SRR4302533.2348932/94‑1 (MQ=60)

                     GTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTG                                                                          <  SRR4302533.4177173/94‑1 (MQ=60)

                       CTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCC                                                                        >  SRR4302533.5794628/1‑94 (MQ=60)

                         CAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCAC                                                                      >  SRR4302533.1257934/1‑94 (MQ=60)

                          AGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAAGCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACG                                                                     <  SRR4302533.3032324/94‑1 (MQ=60)

                            GAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGA                                                                   >  SRR4302533.4629302/1‑94 (MQ=60)

                             AATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGAT                                                                  <  SRR4302533.5187173/94‑1 (MQ=60)

                             AATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGAT                                                                  >  SRR4302533.2540773/1‑94 (MQ=60)

                               TTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTA                                                                >  SRR4302533.5165060/1‑94 (MQ=60)

                                TATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTAT                                                               <  SRR4302533.3177213/94‑1 (MQ=60)

                                TATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTAT                                                               <  SRR4302533.2682423/94‑1 (MQ=60)

                                 ATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATG                                                              >  SRR4302533.3284253/1‑94 (MQ=60)

                                  TCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGG                                                             >  SRR4302533.5435329/1‑94 (MQ=60)

                                  TCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGG                                                             <  SRR4302533.6201356/94‑1 (MQ=60)

                                   CTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGT                                                            <  SRR4302533.5052000/94‑1 (MQ=60)

                                   CTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGT                                                            >  SRR4302533.6105009/1‑94 (MQ=60)

                                   CTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGT                                                            >  SRR4302533.1753152/1‑94 (MQ=60)

                                    TATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTA                                                           <  SRR4302533.365784/94‑1 (MQ=60)

                                    TATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTA                                                           <  SRR4302533.4629773/94‑1 (MQ=60)

                                     ATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAG                                                          <  SRR4302533.4629468/94‑1 (MQ=60)

                                      TCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGC                                                         <  SRR4302533.6338807/94‑1 (MQ=60)

                                        GTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTC                                                       >  SRR4302533.1127974/1‑94 (MQ=60)

                                        GTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTC                                                       <  SRR4302533.3086655/94‑1 (MQ=60)

                                        GTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTC                                                       >  SRR4302533.4439598/1‑94 (MQ=60)

                                         TCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCT                                                      >  SRR4302533.3685489/1‑94 (MQ=60)

                                          CCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTC                                                     >  SRR4302533.1445247/1‑94 (MQ=60)

                                           CGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCC                                                    >  SRR4302533.180760/1‑94 (MQ=60)

                                           CGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCC                                                    >  SRR4302533.4242819/1‑94 (MQ=60)

                                           CGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCC                                                    >  SRR4302533.1867959/1‑94 (MQ=60)

                                            GTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCA                                                   <  SRR4302533.6001075/94‑1 (MQ=60)

                                            GTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCA                                                   >  SRR4302533.5591942/1‑94 (MQ=60)

                                             TCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCAT                                                  >  SRR4302533.4969453/1‑94 (MQ=60)

                                              CGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATC                                                 <  SRR4302533.5089714/94‑1 (MQ=60)

                                               GATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCG                                                >  SRR4302533.5601299/1‑94 (MQ=60)

                                                  TACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCT                                             <  SRR4302533.5854479/94‑1 (MQ=60)

                                                   ACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTG                                            >  SRR4302533.1322470/1‑94 (MQ=60)

                                                    CTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGA                                           >  SRR4302533.5066192/1‑94 (MQ=60)

                                                     TCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGAT                                          <  SRR4302533.2358646/94‑1 (MQ=60)

                                                     TCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGAT                                          <  SRR4302533.2380432/94‑1 (MQ=60)

                                                     TCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGAT                                          <  SRR4302533.4927260/94‑1 (MQ=60)

                                                      CGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATA                                         <  SRR4302533.1078526/94‑1 (MQ=60)

                                                       GATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAG                                        <  SRR4302533.3698336/94‑1 (MQ=60)

                                                       GATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAG                                        >  SRR4302533.4458253/1‑94 (MQ=60)

                                                       CATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAG                                        <  SRR4302533.2231420/94‑1 (MQ=60)

                                                        ATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGA                                       >  SRR4302533.3948116/1‑94 (MQ=60)

                                                        ATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGA                                       >  SRR4302533.4136869/1‑94 (MQ=60)

                                                        ATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGA                                       >  SRR4302533.2661518/1‑94 (MQ=60)

                                                        ATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGA                                       <  SRR4302533.1249876/94‑1 (MQ=60)

                                                         TATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGAT                                      >  SRR4302533.516315/1‑94 (MQ=60)

                                                          ATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATC                                     <  SRR4302533.4489783/94‑1 (MQ=60)

                                                          ATCCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATC                                     <  SRR4302533.1713260/94‑1 (MQ=60)

                                                            CCCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCAC                                   >  SRR4302533.1719285/1‑94 (MQ=60)

                                                             CCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACC                                  <  SRR4302533.1958411/94‑1 (MQ=60)

                                                             CCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACC                                  >  SRR4302533.1133345/1‑94 (MQ=60)

                                                             CCTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACC                                  >  SRR4302533.4873952/1‑94 (MQ=60)

                                                              CTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCT                                 >  SRR4302533.227422/1‑94 (MQ=60)

                                                              CTTTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCT                                 >  SRR4302533.5106334/1‑94 (MQ=60)

                                                                TTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGG                               <  SRR4302533.4433186/94‑1 (MQ=60)

                                                                TTCAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGG                               <  SRR4302533.941063/94‑1 (MQ=60)

                                                                TACAACCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGG                               <  SRR4302533.1144182/94‑1 (MQ=60)

                                                                  CAATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGC                             <  SRR4302533.4933207/94‑1 (MQ=60)

                                                                    ATCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCA                           >  SRR4302533.3544729/1‑94 (MQ=60)

                                                                     TCAACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAG                          <  SRR4302533.9646/94‑1 (MQ=60)

                                                                       AACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCT                        >  SRR4302533.164238/1‑94 (MQ=60)

                                                                       AACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCT                        >  SRR4302533.1837063/1‑94 (MQ=60)

                                                                        ACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTC                       >  SRR4302533.4186928/1‑94 (MQ=60)

                                                                        ACAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTC                       <  SRR4302533.1411792/94‑1 (MQ=60)

                                                                         CAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCA                      >  SRR4302533.660251/1‑94 (MQ=60)

                                                                         CAATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCA                      >  SRR4302533.3527966/1‑94 (MQ=60)

                                                                           ATGAATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATT                    <  SRR4302533.2525892/94‑1 (MQ=60)

                                                                              AATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATT                 <  SRR4302533.1339721/94‑1 (MQ=60)

                                                                               ATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTA                >  SRR4302533.390099/1‑94 (MQ=60)

                                                                               ATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTA                <  SRR4302533.5206192/94‑1 (MQ=60)

                                                                               ATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTA                <  SRR4302533.5433895/94‑1 (MQ=60)

                                                                               ATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTA                <  SRR4302533.2262852/94‑1 (MQ=60)

                                                                               ATCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTA                >  SRR4302533.310728/1‑94 (MQ=60)

                                                                                TCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTAC               <  SRR4302533.2602286/94‑1 (MQ=60)

                                                                                TCATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTAC               <  SRR4302533.4324018/94‑1 (MQ=60)

                                                                                 CATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACT              >  SRR4302533.2124788/1‑94 (MQ=60)

                                                                                 CATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACT              <  SRR4302533.563653/94‑1 (MQ=60)

                                                                                 CATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACT              <  SRR4302533.2538176/94‑1 (MQ=60)

                                                                                 CATCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACT              >  SRR4302533.5105833/1‑94 (MQ=60)

                                                                                   TCCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGTGCCAGCTCATTATTACTGG            >  SRR4302533.4240454/1‑94 (MQ=60)

                                                                                    CCCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGA           >  SRR4302533.2981973/1‑94 (MQ=60)

                                                                                     CCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAC          >  SRR4302533.4823084/1‑94 (MQ=60)

                                                                                     CCCTTCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGAC          >  SRR4302533.2329379/1‑94 (MQ=60)

                                                                                         TCCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGAT      <  SRR4302533.2657299/94‑1 (MQ=60)

                                                                                          CCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATC     >  SRR4302533.4782201/1‑94 (MQ=60)

                                                                                          CCGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATC     >  SRR4302533.5555638/1‑94 (MQ=60)

                                                                                           CGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCA    >  SRR4302533.2657726/1‑94 (MQ=60)

                                                                                           CGAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCA    >  SRR4302533.5572134/1‑94 (MQ=60)

                                                                                            GAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCAT   >  SRR4302533.2664949/1‑94 (MQ=60)

                                                                                            GAAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCAT   <  SRR4302533.5580735/94‑1 (MQ=60)

                                                                                             AAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA  <  SRR4302533.357240/94‑1 (MQ=60)

                                                                                             AAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA  >  SRR4302533.2594853/1‑94 (MQ=60)

CGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA  >  NC_000913/2403674‑2403860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`