Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,797 T→C F128S (TTT→TCT)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,7970TC100.0% 753.2 / NA 233F128S (TTT→TCT) srlEPTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (111/122);  total (111/122)

Only 100 of 236 total aligned reads displayed.

GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824708‑2824886
                                                                                         |                                                                                         
gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        <  2:5490215/93‑1 (MQ=255)
  tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  1:5009846/1‑93 (MQ=255)
   ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     <  1:4659134/93‑1 (MQ=255)
   ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     >  2:2717329/1‑93 (MQ=255)
   ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     <  2:3710721/93‑1 (MQ=255)
    gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    <  1:1726845/93‑1 (MQ=255)
      gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:2863294/1‑93 (MQ=255)
      gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:5817199/1‑93 (MQ=255)
       cAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                 >  1:3263579/1‑93 (MQ=255)
        aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                >  2:2571177/1‑93 (MQ=255)
          tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              >  2:1872269/1‑93 (MQ=255)
          tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              <  1:3180120/93‑1 (MQ=255)
           aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                             >  1:2028884/1‑93 (MQ=255)
             aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           >  2:817950/1‑93 (MQ=255)
              ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                          <  1:472306/93‑1 (MQ=255)
               tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCTCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  1:3612345/93‑1 (MQ=255)
               tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  1:33841/93‑1 (MQ=255)
                gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                        >  2:1040756/1‑93 (MQ=255)
                 tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  2:1091364/1‑93 (MQ=255)
                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  2:5666844/1‑93 (MQ=255)
                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  1:5325303/1‑93 (MQ=255)
                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  2:6078668/1‑93 (MQ=255)
                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  1:4278932/1‑93 (MQ=255)
                   gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCGCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                     <  1:318153/93‑1 (MQ=255)
                    aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    <  2:3870462/93‑1 (MQ=255)
                    aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    <  1:4483016/93‑1 (MQ=255)
                      gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  <  1:6145905/93‑1 (MQ=255)
                      gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  <  2:1469621/93‑1 (MQ=255)
                       atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  2:5468638/93‑1 (MQ=255)
                       atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  2:3808052/93‑1 (MQ=255)
                        tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                <  1:3555863/93‑1 (MQ=255)
                         atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               <  1:2959455/93‑1 (MQ=255)
                          tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              >  2:2980248/1‑93 (MQ=255)
                            tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            >  1:537749/1‑93 (MQ=255)
                            tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            <  2:4340098/93‑1 (MQ=255)
                              tggctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  2:2841189/4‑93 (MQ=255)
                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:4932377/93‑1 (MQ=255)
                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:5072684/93‑1 (MQ=255)
                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:4513870/1‑93 (MQ=255)
                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:3791131/93‑1 (MQ=255)
                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:5802308/1‑93 (MQ=255)
                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAAACCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:4775013/93‑1 (MQ=255)
                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:1351347/1‑93 (MQ=255)
                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:602327/1‑93 (MQ=255)
                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:5599363/1‑93 (MQ=255)
                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:3767202/1‑93 (MQ=255)
                                   ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                     <  2:4622201/93‑1 (MQ=255)
                                     ggCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                                   >  2:4846235/1‑93 (MQ=255)
                                      gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                                                  <  2:1636048/93‑1 (MQ=255)
                                       tgTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGa                                                 <  2:2117772/92‑1 (MQ=255)
                                        gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                <  2:1422377/93‑1 (MQ=255)
                                         tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                               >  1:4000178/1‑93 (MQ=255)
                                            aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            <  2:6020832/93‑1 (MQ=255)
                                             agaataAAACATCACTGTAGTAGGTGATGCGACACCACATCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           <  1:1590626/93‑1 (MQ=255)
                                             agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           <  2:4682182/93‑1 (MQ=255)
                                              gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  1:2511160/1‑93 (MQ=255)
                                              gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  1:4631430/1‑93 (MQ=255)
                                               aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                         <  2:398905/93‑1 (MQ=255)
                                                 gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       >  1:3202320/1‑93 (MQ=255)
                                                 gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       <  1:2428571/93‑1 (MQ=255)
                                                  aaaaCATCACTGTAGTAGGTGATGCGACACCACAGCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                                      <  2:741607/93‑1 (MQ=255)
                                                  aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                                      <  1:1139937/93‑1 (MQ=255)
                                                   aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTGCCGTGGGCCGTGACTATGACGCCAGTAAGAAAACCACCGAACAAAGCGATgg                                     <  2:4302609/93‑1 (MQ=255)
                                                    aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    >  2:4958891/1‑93 (MQ=255)
                                                       aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 <  1:5937160/93‑1 (MQ=255)
                                                        tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGTTGGTTTAc                                <  2:6155988/93‑1 (MQ=255)
                                                        tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                <  2:2472339/93‑1 (MQ=255)
                                                         cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  1:743202/93‑1 (MQ=255)
                                                         cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               >  1:5552713/1‑93 (MQ=255)
                                                         cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  1:6078668/93‑1 (MQ=255)
                                                          aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                              <  1:2058536/93‑1 (MQ=255)
                                                           cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             <  1:1188399/93‑1 (MQ=255)
                                                           cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             <  1:4220125/93‑1 (MQ=255)
                                                            tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:3522822/93‑1 (MQ=255)
                                                            tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  1:4796333/93‑1 (MQ=255)
                                                             gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           >  2:5534762/1‑93 (MQ=255)
                                                               agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                         >  1:1483432/1‑93 (MQ=255)
                                                                gtagGTGTTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                        <  1:265742/93‑1 (MQ=255)
                                                                gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                        >  2:337972/1‑93 (MQ=255)
                                                                 tagGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       <  1:2222486/93‑1 (MQ=255)
                                                                 tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       <  2:3108778/93‑1 (MQ=255)
                                                                 tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       >  1:6191585/1‑93 (MQ=255)
                                                                   ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     >  1:2770993/1‑93 (MQ=255)
                                                                       aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:3468966/93‑1 (MQ=255)
                                                                         gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg               <  2:1930216/93‑1 (MQ=255)
                                                                         gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg               <  2:2659047/93‑1 (MQ=255)
                                                                           gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             >  2:2325637/1‑93 (MQ=255)
                                                                           gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             <  1:1658518/93‑1 (MQ=255)
                                                                             caccacACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           <  2:455397/93‑1 (MQ=255)
                                                                             caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           >  1:5204903/1‑93 (MQ=255)
                                                                              accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt          <  2:3286667/93‑1 (MQ=255)
                                                                               ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg         <  2:5116654/93‑1 (MQ=255)
                                                                               ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg         <  1:847388/93‑1 (MQ=255)
                                                                                 acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       >  2:5364935/1‑93 (MQ=255)
                                                                                 acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  1:335414/93‑1 (MQ=255)
                                                                                 acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       >  2:5889385/1‑93 (MQ=255)
                                                                                  caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg      <  2:367212/93‑1 (MQ=255)
                                                                                   aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGt     >  1:2357932/1‑93 (MQ=255)
                                                                                    aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTc    >  1:3710720/1‑93 (MQ=255)
                                                                                     cccTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTcc   <  1:5530689/93‑1 (MQ=255)
                                                                                         |                                                                                         
GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824708‑2824886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  NC_000913/2824705‑2824890
                                                                                            |                                                                                             
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCT                                                                                              >  SRR4302533.4077649/1‑94 (MQ=60)
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCT                                                                                              <  SRR4302533.3415845/94‑1 (MQ=60)
 CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCCCTGTAGTAGGTGATGCGACACCACAACCCTCTT                                                                                             <  SRR4302533.1547489/94‑1 (MQ=60)
   GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                           <  SRR4302533.844979/94‑1 (MQ=60)
   GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                           >  SRR4302533.718104/1‑94 (MQ=60)
    CTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCG                                                                                          >  SRR4302533.5040198/1‑94 (MQ=60)
      GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                        >  SRR4302533.3388374/1‑94 (MQ=60)
      GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                        <  SRR4302533.4687368/94‑1 (MQ=60)
       GCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGG                                                                                       <  SRR4302533.1737327/94‑1 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      >  SRR4302533.2880905/1‑94 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      >  SRR4302533.2880906/1‑94 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      >  SRR4302533.5852341/1‑94 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGCGGG                                                                                      >  SRR4302533.267416/1‑94 (MQ=60)
         GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                     >  SRR4302533.3283710/1‑94 (MQ=60)
          CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    >  SRR4302533.379890/1‑94 (MQ=60)
           AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                   >  SRR4302533.4695501/1‑94 (MQ=60)
            GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGT                                                                                  >  SRR4302533.841262/1‑94 (MQ=60)
             TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                 >  SRR4302533.2041408/1‑94 (MQ=60)
             TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                 <  SRR4302533.3199682/94‑1 (MQ=60)
              ACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGA                                                                                <  SRR4302533.3247932/94‑1 (MQ=60)
               CATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAC                                                                               >  SRR4302533.142676/1‑94 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                              >  SRR4302533.1720674/1‑94 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                              >  SRR4302533.3246787/1‑94 (MQ=60)
                 TTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTA                                                                             <  SRR4302533.475131/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCTCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302533.3634577/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302533.34070/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302533.1189643/94‑1 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                          >  SRR4302533.4304663/1‑94 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                          >  SRR4302533.5357216/1‑94 (MQ=60)
                     GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGAC                                                                         >  SRR4302533.5409610/1‑94 (MQ=60)
                     GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGAC                                                                         >  SRR4302533.3598881/1‑94 (MQ=60)
                     GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGAC                                                                         <  SRR4302533.5577955/94‑1 (MQ=60)
                      GAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCGCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACA                                                                        <  SRR4302533.320103/94‑1 (MQ=60)
                      GAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACA                                                                        >  SRR4302533.344490/1‑94 (MQ=60)
                       AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                       <  SRR4302533.4510164/94‑1 (MQ=60)
                        AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                      <  SRR4302533.3217525/94‑1 (MQ=60)
                         GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                     <  SRR4302533.2459334/94‑1 (MQ=60)
                         GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                     <  SRR4302533.6182907/94‑1 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                    >  SRR4302533.3595197/1‑94 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                    <  SRR4302533.5000130/94‑1 (MQ=60)
                           TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                   <  SRR4302533.3577779/94‑1 (MQ=60)
                            ATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTA                                                                  <  SRR4302533.2977544/94‑1 (MQ=60)
                            ATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTA                                                                  >  SRR4302533.4579051/1‑94 (MQ=60)
                             TTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                                 >  SRR4302533.5051463/1‑94 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                >  SRR4302533.2404478/1‑94 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                >  SRR4302533.540949/1‑94 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                <  SRR4302533.2737056/94‑1 (MQ=60)
                              TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                <  SRR4302533.5630581/94‑1 (MQ=60)
                               TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                               <  SRR4302533.5534841/94‑1 (MQ=60)
                               TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                               >  SRR4302533.4803535/1‑94 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              >  SRR4302533.2048213/1‑94 (MQ=60)
                                 TGTCTCTGGCGTAAAAGAATAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAA                                                             <  SRR4302533.2501909/94‑1 (MQ=60)
                                   TCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAT                                                           >  SRR4302533.4541204/1‑94 (MQ=60)
                                   TCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAT                                                           >  SRR4302533.5837357/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          <  SRR4302533.3814344/94‑1 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          <  SRR4302533.5103344/94‑1 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          <  SRR4302533.4962246/94‑1 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          >  SRR4302533.2822/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          >  SRR4302533.3790299/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          >  SRR4302533.605868/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          >  SRR4302533.5633194/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          >  SRR4302533.1359475/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAAACCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          <  SRR4302533.4803943/94‑1 (MQ=60)
                                     TCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCA                                                         <  SRR4302533.5108222/94‑1 (MQ=60)
                                          CGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                    <  SRR4302533.590234/94‑1 (MQ=60)
                                           GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   >  SRR4302533.425996/1‑94 (MQ=60)
                                           GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   <  SRR4302533.4817035/94‑1 (MQ=60)
                                           GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   >  SRR4302533.4024535/1‑94 (MQ=60)
                                             AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                                 >  SRR4302533.4407403/1‑94 (MQ=60)
                                              AAAGAAGAAAACATCACTGTAGTAGGCGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATTACACCAGTAAGAAAATCACCGAACAAA                                                >  SRR4302533.5682356/1‑94 (MQ=60)
                                                AGAATAAAACATCACTGTAGTAGGTGATGCGACACCACATCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              <  SRR4302533.1600246/94‑1 (MQ=60)
                                                AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              >  SRR4302533.2526634/1‑94 (MQ=60)
                                                AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              >  SRR4302533.4659496/1‑94 (MQ=60)
                                                  AAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGA                                            <  SRR4302533.5480028/94‑1 (MQ=60)
                                                   AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                           >  SRR4302533.3222029/1‑94 (MQ=60)
                                                   AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                           >  SRR4302533.155246/1‑94 (MQ=60)
                                                   AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                           >  SRR4302533.3433913/1‑94 (MQ=60)
                                                    GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGGCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                          >  SRR4302533.17156/1‑94 (MQ=60)
                                                    GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                          <  SRR4302533.2443585/94‑1 (MQ=60)
                                                     AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                         >  SRR4302533.394388/1‑94 (MQ=60)
                                                     AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                         <  SRR4302533.5845107/94‑1 (MQ=60)
                                                     AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                         <  SRR4302533.1146719/94‑1 (MQ=60)
                                                      AAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGT                                        >  SRR4302533.6068101/1‑94 (MQ=60)
                                                      AAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGT                                        >  SRR4302533.4665772/1‑94 (MQ=60)
                                                          ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                    <  SRR4302533.5973058/94‑1 (MQ=60)
                                                           TCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACT                                   >  SRR4302533.4610251/1‑94 (MQ=60)
                                                           TCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACT                                   >  SRR4302533.5586245/1‑94 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  <  SRR4302533.6115433/94‑1 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  >  SRR4302533.3023436/1‑94 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  <  SRR4302533.747577/94‑1 (MQ=60)
                                                             ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                 <  SRR4302533.2071228/94‑1 (MQ=60)
                                                              CTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGC                                <  SRR4302533.3812509/94‑1 (MQ=60)
                                                              CTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGC                                <  SRR4302533.1195533/94‑1 (MQ=60)
                                                              CTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGC                                >  SRR4302533.1292647/1‑94 (MQ=60)
                                                              CTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGC                                <  SRR4302533.4245643/94‑1 (MQ=60)
                                                               TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                               <  SRR4302533.4825386/94‑1 (MQ=60)
                                                                GTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGA                              <  SRR4302533.4088419/94‑1 (MQ=60)
                                                                 TAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAA                             >  SRR4302533.1492387/1‑94 (MQ=60)
                                                                  AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                            >  SRR4302533.3691292/1‑94 (MQ=60)
                                                                  AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                            <  SRR4302533.3043235/94‑1 (MQ=60)
                                                                   GTAGGTGTTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGG                           <  SRR4302533.267359/94‑1 (MQ=60)
                                                                   GTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGG                           >  SRR4302533.6228743/1‑94 (MQ=60)
                                                                    TAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGT                          <  SRR4302533.2236215/94‑1 (MQ=60)
                                                                    TAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGT                          <  SRR4302533.2556899/94‑1 (MQ=60)
                                                                     AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG                         >  SRR4302533.1441635/1‑94 (MQ=60)
                                                                     AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG                         >  SRR4302533.2787993/1‑94 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                        <  SRR4302533.5954063/94‑1 (MQ=60)
                                                                        TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                      >  SRR4302533.5127115/1‑94 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                    <  SRR4302533.3490381/94‑1 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                    <  SRR4302533.3738622/94‑1 (MQ=60)
                                                                            GCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGG                  <  SRR4302533.1827129/94‑1 (MQ=60)
                                                                            GCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGG                  <  SRR4302533.4874592/94‑1 (MQ=60)
                                                                              GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                <  SRR4302533.3317751/94‑1 (MQ=60)
                                                                              GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                <  SRR4302533.1668551/94‑1 (MQ=60)
                                                                              GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                >  SRR4302533.2310630/1‑94 (MQ=60)
                                                                               ACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCA               >  SRR4302533.5236266/1‑94 (MQ=60)
                                                                                CACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAT              <  SRR4302533.4272992/94‑1 (MQ=60)
                                                                                 ACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATG             <  SRR4302533.2109952/94‑1 (MQ=60)
                                                                                  CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG            <  SRR4302533.6017142/94‑1 (MQ=60)
                                                                                  CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG            <  SRR4302533.852349/94‑1 (MQ=60)
                                                                                  CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG            <  SRR4302533.3201071/94‑1 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           >  SRR4302533.6199644/1‑94 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           <  SRR4302533.162339/94‑1 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           <  SRR4302533.2227336/94‑1 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           <  SRR4302533.6180094/94‑1 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           >  SRR4302533.611451/1‑94 (MQ=60)
                                                                                    ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG          <  SRR4302533.337464/94‑1 (MQ=60)
                                                                                     CAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGT         >  SRR4302533.2372507/1‑94 (MQ=60)
                                                                                     CAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGT         >  SRR4302533.3538136/1‑94 (MQ=60)
                                                                                      AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC        >  SRR4302533.3733509/1‑94 (MQ=60)
                                                                                       ACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCC       <  SRR4302533.5708371/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA      <  SRR4302533.53311/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA      <  SRR4302533.5564078/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA      <  SRR4302533.4924635/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA      <  SRR4302533.4522498/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA      <  SRR4302533.4788909/94‑1 (MQ=60)
                                                                                         CCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGcactgggatgcggtgcac     >  SRR4302533.452079/1‑76 (MQ=60)
                                                                                          CTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC    <  SRR4302533.2028753/94‑1 (MQ=60)
                                                                                          CTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC    <  SRR4302533.2097555/94‑1 (MQ=60)
                                                                                           TCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG   >  SRR4302533.2394684/1‑94 (MQ=60)
                                                                                            CTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  <  SRR4302533.47697/94‑1 (MQ=60)
                                                                                            |                                                                                             
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  NC_000913/2824705‑2824890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑