Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,941,864 T→C M169T (ATG→ACG)  b2810 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,941,8640TC99.6% 780.6 / ‑4.3 240M169T (ATG→ACG) b2810hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (122/117);  minor base A (0/1);  total (122/118)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 248 total aligned reads displayed.

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGC  >  NC_000913/2941774‑2941951
                                                                                          |                                                                                       
aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                       <  1:6670/93‑1 (MQ=255)
aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                       <  1:1947145/93‑1 (MQ=255)
aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                       >  2:6240664/1‑93 (MQ=255)
 aTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTc                                                                                      >  1:3787427/1‑93 (MQ=255)
  ttGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCg                                                                                     >  1:926860/1‑93 (MQ=255)
     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAc                                                                                  >  2:2667862/1‑93 (MQ=255)
     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAc                                                                                  >  1:3564904/1‑93 (MQ=255)
     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGCTGGGTCAGACGTCGAAc                                                                                  >  2:3425136/1‑93 (MQ=255)
       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                >  2:4002774/1‑93 (MQ=255)
       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                <  2:4107109/93‑1 (MQ=255)
         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                              >  1:1356162/1‑93 (MQ=255)
         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                              >  1:2490753/1‑93 (MQ=255)
            aTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTg                                                                           <  1:892468/93‑1 (MQ=255)
              gcgcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGc                                                                         <  2:1325250/93‑1 (MQ=255)
                gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                       >  2:2124303/1‑93 (MQ=255)
                gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                       >  1:5464771/1‑93 (MQ=255)
                 cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                      >  2:4660016/1‑93 (MQ=255)
                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgggt                                                                     >  2:4368674/1‑91 (MQ=255)
                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                     <  1:801286/93‑1 (MQ=255)
                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    >  1:2823396/1‑93 (MQ=255)
                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    >  2:3553528/1‑93 (MQ=255)
                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    <  2:2167881/93‑1 (MQ=255)
                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    >  2:598709/1‑93 (MQ=255)
                     gACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTATGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTggc                                                                  >  1:107988/1‑91 (MQ=255)
                        tGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCgg                                                               <  1:856219/93‑1 (MQ=255)
                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                              >  2:43804/1‑93 (MQ=255)
                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGac                                                             >  1:2143459/1‑92 (MQ=255)
                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                             <  1:2580490/93‑1 (MQ=255)
                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                             <  2:4996744/93‑1 (MQ=255)
                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                             <  2:6153742/93‑1 (MQ=255)
                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            <  1:2209646/93‑1 (MQ=255)
                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            >  2:419596/1‑93 (MQ=255)
                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            >  2:4493732/1‑93 (MQ=255)
                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            <  1:2587351/93‑1 (MQ=255)
                            ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCt                                                           >  1:440952/1‑93 (MQ=255)
                            ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCt                                                           >  2:372032/1‑93 (MQ=255)
                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCCGCTCTg                                                          >  2:718757/1‑93 (MQ=255)
                               tGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgc                                                        <  1:5141717/93‑1 (MQ=255)
                                 tCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgcgc                                                      <  1:536598/93‑1 (MQ=255)
                                  cGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcg                                                     >  2:2519875/1‑93 (MQ=255)
                                  cGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcg                                                     <  1:5693208/93‑1 (MQ=255)
                                  cGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGCCCGGATCTGGcgcg                                                     >  2:1848393/1‑93 (MQ=255)
                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGcgcgg                                                    >  2:3234938/1‑92 (MQ=255)
                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcgg                                                    >  1:412816/1‑92 (MQ=255)
                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                    <  1:2051097/93‑1 (MQ=255)
                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                    <  1:6301777/93‑1 (MQ=255)
                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                    >  1:4521301/1‑93 (MQ=255)
                                    aTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgag                                                   >  2:6280499/1‑93 (MQ=255)
                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagc                                                  >  1:4664589/1‑93 (MQ=255)
                                      ttgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcg                                                 <  1:4764005/93‑1 (MQ=255)
                                       tgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcga                                                >  2:1737689/1‑93 (MQ=255)
                                        gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAt                                               >  2:1498446/1‑93 (MQ=255)
                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                              <  2:3937666/93‑1 (MQ=255)
                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                              >  1:747292/1‑93 (MQ=255)
                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          <  2:3673654/93‑1 (MQ=255)
                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          >  1:2894077/1‑93 (MQ=255)
                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          <  2:5572956/93‑1 (MQ=255)
                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          <  2:5391025/93‑1 (MQ=255)
                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                         <  1:3849824/93‑1 (MQ=255)
                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                         <  1:6013133/93‑1 (MQ=255)
                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                         >  1:1865858/1‑93 (MQ=255)
                                                     aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGATTACCTTTGCTCAt                                  >  1:711229/1‑93 (MQ=255)
                                                     aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAt                                  <  1:5854495/93‑1 (MQ=255)
                                                        aCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                               <  2:2023974/93‑1 (MQ=255)
                                                        aCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                               <  1:625423/93‑1 (MQ=255)
                                                          tCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGc                             >  1:2373178/1‑93 (MQ=255)
                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGcc                            <  2:5298380/93‑1 (MQ=255)
                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGcc                            <  1:2988792/93‑1 (MQ=255)
                                                            cccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCg                           >  2:2403336/1‑93 (MQ=255)
                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                          >  1:578442/1‑93 (MQ=255)
                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                          <  2:3078144/93‑1 (MQ=255)
                                                                tAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAt                       <  1:4958620/93‑1 (MQ=255)
                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGGTTACCTTTGCTCATTCAGCCGGGGtg                      >  2:2282457/1‑93 (MQ=255)
                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtg                      <  1:3257220/93‑1 (MQ=255)
                                                                  gTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGCtgg                     >  1:3519221/1‑93 (MQ=255)
                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggt                    <  2:514587/93‑1 (MQ=255)
                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggt                    <  2:620984/93‑1 (MQ=255)
                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                    >  1:4831927/1‑92 (MQ=255)
                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                    >  1:1670741/1‑92 (MQ=255)
                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                    >  2:1599009/1‑92 (MQ=255)
                                                                    cGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtg                   <  2:425446/93‑1 (MQ=255)
                                                                     ggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                  >  2:1469538/1‑93 (MQ=255)
                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtgggggg                 >  2:2974883/1‑92 (MQ=255)
                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                 <  2:924068/93‑1 (MQ=255)
                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                 >  1:5637183/1‑93 (MQ=255)
                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtga               >  1:3614599/1‑93 (MQ=255)
                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGTCCGGATCCGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGGtggtggggg               >  1:4305645/1‑90 (MQ=255)
                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGGCCGGCTCTGGCGCGAGCGATTACCTCTGCTCCTTCAGCCGGGATGGTGGtga               >  2:3017362/1‑93 (MQ=255)
                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat              <  1:130191/93‑1 (MQ=255)
                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat              <  2:772233/93‑1 (MQ=255)
                                                                           tGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatgg            <  1:2729361/93‑1 (MQ=255)
                                                                           tGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatgg            >  1:1057274/1‑93 (MQ=255)
                                                                            ggCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggt           <  2:5795192/93‑1 (MQ=255)
                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       <  1:338725/93‑1 (MQ=255)
                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       >  2:872457/1‑93 (MQ=255)
                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       <  1:1001880/93‑1 (MQ=255)
                                                                                gtGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       <  1:584222/92‑1 (MQ=255)
                                                                                  gggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatgg     <  2:6030239/93‑1 (MQ=255)
                                                                                    gTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTg   >  2:1265580/1‑93 (MQ=255)
                                                                                     tCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGGGGTGATGGTTGATGGTGc  >  1:3247013/1‑93 (MQ=255)
                                                                                          |                                                                                       
AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGC  >  NC_000913/2941774‑2941951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGG  >  NC_000913/2941771‑2941957
                                                                                             |                                                                                             
TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGAC                                                                                               <  SRR4302533.174303/94‑1 (MQ=60)
   AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTC                                                                                            <  SRR4302533.1959016/94‑1 (MQ=60)
   AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTC                                                                                            >  SRR4302533.3810617/1‑94 (MQ=60)
   AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTC                                                                                            <  SRR4302533.6730/94‑1 (MQ=60)
   AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTC                                                                                            >  SRR4302533.3751713/1‑94 (MQ=60)
    ATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCG                                                                                           >  SRR4302533.932299/1‑94 (MQ=60)
       GCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAC                                                                                        >  SRR4302533.3586875/1‑94 (MQ=60)
         CGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGT                                                                                      >  SRR4302533.4855478/1‑94 (MQ=60)
         CGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGT                                                                                      >  SRR4302533.4992430/1‑94 (MQ=60)
           CTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTA                                                                                    >  SRR4302533.1364314/1‑94 (MQ=60)
           CTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTA                                                                                    >  SRR4302533.2506096/1‑94 (MQ=60)
             TAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGAAGTCGAACGTTACT                                                                                  >  SRR4302533.5363731/1‑94 (MQ=60)
              AATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCTGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTG                                                                                 >  SRR4302533.1205539/1‑94 (MQ=60)
              AATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTG                                                                                 <  SRR4302533.6013165/94‑1 (MQ=60)
               ATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGG                                                                                >  SRR4302533.2627934/1‑94 (MQ=60)
               ATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGG                                                                                <  SRR4302533.897714/94‑1 (MQ=60)
                  CGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGG                                                                             >  SRR4302533.5497701/1‑94 (MQ=60)
                     AGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTG                                                                          <  SRR4302533.5374281/94‑1 (MQ=60)
                     AGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTG                                                                          <  SRR4302533.805998/94‑1 (MQ=60)
                     AGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTG                                                                          >  SRR4302533.2840769/1‑94 (MQ=60)
                     AGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTG                                                                          <  SRR4302533.2912181/94‑1 (MQ=60)
                       CGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTATGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGgtggc                                                                        >  SRR4302533.108667/1‑89 (MQ=60)
                        GACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCC                                                                       <  SRR4302533.4750810/94‑1 (MQ=60)
                           TGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGA                                                                    <  SRR4302533.861234/94‑1 (MQ=60)
                            GCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAT                                                                   <  SRR4302533.867955/94‑1 (MQ=60)
                            GCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAT                                                                   <  SRR4302533.1569025/94‑1 (MQ=60)
                            GCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAC                                                                   >  SRR4302533.2156665/1‑94 (MQ=60)
                             CCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATC                                                                  <  SRR4302533.2860426/94‑1 (MQ=60)
                             CCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATC                                                                  <  SRR4302533.2596369/94‑1 (MQ=60)
                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCT                                                                 >  SRR4302533.443584/1‑94 (MQ=60)
                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302533.3508163/94‑1 (MQ=60)
                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302533.3378623/94‑1 (MQ=60)
                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302533.2603257/94‑1 (MQ=60)
                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302533.2223273/94‑1 (MQ=60)
                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGCGGCCCGGGTCT                                                                 >  SRR4302533.5702712/1‑94 (MQ=60)
                                GATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGG                                                               >  SRR4302533.4478759/1‑94 (MQ=60)
                                GATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGG                                                               <  SRR4302533.4722468/94‑1 (MQ=60)
                                 ATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGC                                                              <  SRR4302533.3588132/94‑1 (MQ=60)
                                  TGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCG                                                             <  SRR4302533.5728299/94‑1 (MQ=60)
                                  TGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCG                                                             <  SRR4302533.5172791/94‑1 (MQ=60)
                                   GTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGgtttggggg                                                            >  SRR4302533.762284/1‑85 (MQ=60)
                                    TCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCG                                                           >  SRR4302533.5611843/1‑94 (MQ=60)
                                    TCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCG                                                           <  SRR4302533.539786/94‑1 (MQ=60)
                                     CGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGA                                                          >  SRR4302533.4536975/1‑94 (MQ=60)
                                     CGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGG                                                          >  SRR4302533.415297/1‑94 (MQ=60)
                                     CGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGA                                                          >  SRR4302533.4548677/1‑94 (MQ=60)
                                     CGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGA                                                          <  SRR4302533.5727589/94‑1 (MQ=60)
                                      GCTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGCCCGGATCTGGCGCGCG                                                         >  SRR4302533.1877023/1‑94 (MQ=60)
                                      GATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAGGTTACTGGCGGTTGCCCGGATCTGGCGCGCG                                                         >  SRR4302533.3770834/1‑94 (MQ=60)
                                      GATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAG                                                         <  SRR4302533.6339421/94‑1 (MQ=60)
                                      GATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAG                                                         <  SRR4302533.5661507/94‑1 (MQ=60)
                                      GATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAG                                                         <  SRR4302533.576377/94‑1 (MQ=60)
                                      GATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAG                                                         <  SRR4302533.2063749/94‑1 (MQ=60)
                                       ATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGC                                                        >  SRR4302533.4692850/1‑94 (MQ=60)
                                       ATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGC                                                        <  SRR4302533.1411180/94‑1 (MQ=60)
                                        TTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCG                                                       >  SRR4302533.1081720/1‑94 (MQ=60)
                                         TTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGA                                                      <  SRR4302533.4792897/94‑1 (MQ=60)
                                           GTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATT                                                    >  SRR4302533.751692/1‑94 (MQ=60)
                                            TTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGGGAGCGATTA                                                   >  SRR4302533.4084188/1‑94 (MQ=60)
                                            TTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGCGCGAATA                                                   >  SRR4302533.5879339/1‑94 (MQ=60)
                                             TGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAC                                                  <  SRR4302533.3572633/94‑1 (MQ=60)
                                             TGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAC                                                  >  SRR4302533.304447/1‑94 (MQ=60)
                                             TGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAC                                                  <  SRR4302533.2574973/94‑1 (MQ=60)
                                             TGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAC                                                  <  SRR4302533.336047/94‑1 (MQ=60)
                                              GCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACC                                                 >  SRR4302533.4964856/1‑94 (MQ=60)
                                              GCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACC                                                 >  SRR4302533.3024764/1‑94 (MQ=60)
                                              GCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACC                                                 >  SRR4302533.2196331/1‑94 (MQ=60)
                                               CCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCT                                                >  SRR4302533.2911859/1‑94 (MQ=60)
                                               CCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCT                                                >  SRR4302533.6322430/1‑94 (MQ=60)
                                                CAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTT                                               >  SRR4302533.1877237/1‑94 (MQ=60)
                                                 AGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTT                                              <  SRR4302533.713275/94‑1 (MQ=60)
                                                 AGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTT                                              <  SRR4302533.6049563/94‑1 (MQ=60)
                                                 AGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTT                                              <  SRR4302533.3873333/94‑1 (MQ=60)
                                                 AGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTT                                              <  SRR4302533.2556111/94‑1 (MQ=60)
                                                  GAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTG                                             <  SRR4302533.2821165/94‑1 (MQ=60)
                                                  GAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGCTGCCCGGATCTGGCGCGAGCGATTACCTTTG                                             >  SRR4302533.6346749/1‑94 (MQ=60)
                                                   AACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGC                                            >  SRR4302533.921477/1‑94 (MQ=60)
                                                   AACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGC                                            >  SRR4302533.4199567/1‑94 (MQ=60)
                                                     CTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTC                                          >  SRR4302533.435873/1‑94 (MQ=60)
                                                     CTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTC                                          <  SRR4302533.588571/94‑1 (MQ=60)
                                                      TGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCA                                         >  SRR4302533.831493/1‑94 (MQ=60)
                                                       GATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGATTACCTTTGCTCAT                                        >  SRR4302533.715429/1‑94 (MQ=60)
                                                        ATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATT                                       <  SRR4302533.5889891/94‑1 (MQ=60)
                                                         TTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCCGGAGCGCTTACTTTTGCTCATTC                                      >  SRR4302533.719035/1‑94 (MQ=60)
                                                         TTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTC                                      >  SRR4302533.2478223/1‑94 (MQ=60)
                                                          TACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCA                                     <  SRR4302533.2123907/94‑1 (MQ=60)
                                                           ACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAG                                    <  SRR4302533.629095/94‑1 (MQ=60)
                                                            CTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGC                                   >  SRR4302533.2387865/1‑94 (MQ=60)
                                                              CCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCG                                 >  SRR4302533.1018663/1‑94 (MQ=60)
                                                              CCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCG                                 <  SRR4302533.3007076/94‑1 (MQ=60)
                                                               CCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGG                                <  SRR4302533.4639154/94‑1 (MQ=60)
                                                               CCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGG                                >  SRR4302533.581852/1‑94 (MQ=60)
                                                               CCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGG                                <  SRR4302533.2627864/94‑1 (MQ=60)
                                                                CCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGG                               <  SRR4302533.1179145/94‑1 (MQ=60)
                                                                CCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGG                               <  SRR4302533.188115/94‑1 (MQ=60)
                                                                 CGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGA                              >  SRR4302533.668609/1‑94 (MQ=60)
                                                                   TAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATG                            <  SRR4302533.4988658/94‑1 (MQ=60)
                                                                    AGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGCTGG                           >  SRR4302533.3540917/1‑94 (MQ=60)
                                                                    AGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGG                           <  SRR4302533.3277308/94‑1 (MQ=60)
                                                                    AGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGG                           <  SRR4302533.2116465/94‑1 (MQ=60)
                                                                     GTCGGATTCTGGCGTTGGGTCAGCCGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGT                          <  SRR4302533.3273161/94‑1 (MQ=60)
                                                                     GTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGG                          >  SRR4302533.1680841/1‑94 (MQ=60)
                                                                     GTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGG                          >  SRR4302533.4861213/1‑94 (MQ=60)
                                                                       CGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGG                        <  SRR4302533.2893892/94‑1 (MQ=60)
                                                                       CGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGGGG                        >  SRR4302533.4167944/1‑94 (MQ=60)
                                                                        GGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGT                       >  SRR4302533.5671269/1‑94 (MQ=60)
                                                                        GGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGT                       >  SRR4302533.3813452/1‑94 (MQ=60)
                                                                         GATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTG                      >  SRR4302533.1904162/1‑94 (MQ=60)
                                                                          ATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGA                     >  SRR4302533.3636841/1‑94 (MQ=60)
                                                                          ATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGTCCGGATCCGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGGTGGTGGggg                     >  SRR4302533.4331552/1‑91 (MQ=60)
                                                                           TTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGAT                    <  SRR4302533.619848/94‑1 (MQ=60)
                                                                            TCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATG                   >  SRR4302533.1311782/1‑94 (MQ=60)
                                                                            TCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATG                   <  SRR4302533.5848877/94‑1 (MQ=60)
                                                                            TCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATG                   <  SRR4302533.6231197/94‑1 (MQ=60)
                                                                            TCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATG                   <  SRR4302533.131006/94‑1 (MQ=60)
                                                                             CTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGG                  <  SRR4302533.821127/94‑1 (MQ=60)
                                                                             CTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGG                  >  SRR4302533.1063490/1‑94 (MQ=60)
                                                                             CTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGG                  >  SRR4302533.5427483/1‑94 (MQ=60)
                                                                              TGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGT                 <  SRR4302533.2746102/94‑1 (MQ=60)
                                                                                GCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTG               <  SRR4302533.2007021/94‑1 (MQ=60)
                                                                                  GTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGAT             <  SRR4302533.3582503/94‑1 (MQ=60)
                                                                                  GTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGAT             >  SRR4302533.2346641/1‑94 (MQ=60)
                                                                                   TTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATG            <  SRR4302533.1007730/94‑1 (MQ=60)
                                                                                   TTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATG            <  SRR4302533.340789/94‑1 (MQ=60)
                                                                                   TTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATG            <  SRR4302533.2602680/94‑1 (MQ=60)
                                                                                   GTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATG            <  SRR4302533.587667/94‑1 (MQ=60)
                                                                                     GGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGT          <  SRR4302533.4980494/94‑1 (MQ=60)
                                                                                       GTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGC        >  SRR4302533.735764/1‑94 (MQ=60)
                                                                                       GTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGGGGTGATGGTTGATGGTGC        >  SRR4302533.3267037/1‑94 (MQ=60)
                                                                                          AGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCA     >  SRR4302533.3257527/1‑94 (MQ=60)
                                                                                           GACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAG    >  SRR4302533.4359505/1‑94 (MQ=60)
                                                                                             CGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGG  <  SRR4302533.1238964/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGG  >  NC_000913/2941771‑2941957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑