Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,979,391 T→C T272A (ACC→GCC)  araE ← low‑affinity L‑arabinose transport system proton symport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,979,3910TC100.0% 752.4 / NA 232T272A (ACC→GCC) araElow‑affinity L‑arabinose transport system proton symport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (105/127);  total (105/127)

Only 100 of 240 total aligned reads displayed.

CCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT  >  NC_000913/2979301‑2979480
                                                                                          |                                                                                         
 cAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATATGCGGCGCGTAGTACATGATGATGTTCATACCGGCaac                                                                                        >  2:5206718/1‑92 (MQ=255)
 cAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTTATACCGGCaaa                                                                                        >  2:5571832/1‑93 (MQ=255)
 cAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCaaa                                                                                        >  2:5225480/1‑93 (MQ=255)
 cAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCaaa                                                                                        >  2:3307557/1‑93 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  1:1579320/93‑1 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  1:197938/93‑1 (MQ=255)
   gagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAct                                                                                      >  2:37861/1‑93 (MQ=255)
   gagTCGCAATCATCAGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAct                                                                                      >  2:3847971/1‑93 (MQ=255)
    agTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAActg                                                                                     <  2:5913656/93‑1 (MQ=255)
     gTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAActgc                                                                                    <  2:136875/93‑1 (MQ=255)
      tCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATATCGGCAAActgct                                                                                   <  1:3406328/93‑1 (MQ=255)
       cGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAActgctg                                                                                  >  2:1868847/1‑93 (MQ=255)
        gCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACtgctgc                                                                                 <  1:5908546/93‑1 (MQ=255)
         cAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCa                                                                                >  1:1380781/1‑93 (MQ=255)
          aaTCATCTGTTGTTCTGTGGGCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCac                                                                               >  1:5809806/1‑92 (MQ=255)
           atcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATc                                                                              >  2:398519/1‑93 (MQ=255)
           atcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATc                                                                              <  1:422518/93‑1 (MQ=255)
            tcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCg                                                                             >  1:289474/1‑93 (MQ=255)
            tcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCg                                                                             >  2:5639875/1‑93 (MQ=255)
               tcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCt                                                                          <  1:4825154/93‑1 (MQ=255)
               tcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCt                                                                          >  2:1751758/1‑93 (MQ=255)
               tcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCt                                                                          >  1:1134791/1‑93 (MQ=255)
                 tgttgtTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCttc                                                                        <  2:6211464/93‑3 (MQ=255)
                 tgttgtTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGc                                                                        <  2:2600559/93‑1 (MQ=255)
                   ttgttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGcaa                                                                      <  1:3942837/93‑1 (MQ=255)
                   ttgttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGcaa                                                                      >  2:3907900/1‑93 (MQ=255)
                    tgttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTCGTACATGATGATGTTCATACCGTCAAACTGCTGCATCGCCTGcaac                                                                     <  2:5073157/93‑1 (MQ=255)
                      ttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGcaacaa                                                                   <  2:2080113/93‑1 (MQ=255)
                        cTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAacaaca                                                                 <  2:4616800/93‑1 (MQ=255)
                        cTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAacaaca                                                                 <  1:4013607/93‑1 (MQ=255)
                         tgtgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAt                                                                <  2:3011078/93‑1 (MQ=255)
                         tgtgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAt                                                                >  2:1929520/1‑93 (MQ=255)
                           tgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAc                                                              <  1:3506435/93‑1 (MQ=255)
                           tgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAc                                                              >  1:5585881/1‑93 (MQ=255)
                            ggTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAcc                                                             <  1:5403775/93‑1 (MQ=255)
                              tCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCga                                                           <  2:5545740/93‑1 (MQ=255)
                                 tAAAGGCCGCCATTTTGAAGATACGCGGCGCGTAGGACATGATGATGTTCATACCGGCAAACTGCTGCATCGCGTGCAACAAAATACCGAGaa                                                        >  1:1888060/1‑93 (MQ=255)
                                 tAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaa                                                        <  1:4063513/93‑1 (MQ=255)
                                 tAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaa                                                        >  1:2286001/1‑93 (MQ=255)
                                  aaaGCCCGCCATTTTGAAGATACGCGGCGTGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaaa                                                       <  2:745831/93‑1 (MQ=255)
                                  aaaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaaa                                                       <  2:306537/93‑1 (MQ=255)
                                  aaaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaaa                                                       <  2:5374142/93‑1 (MQ=255)
                                  aaaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaaa                                                       >  1:3887100/1‑93 (MQ=255)
                                         gCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCa                                                <  2:1535564/93‑1 (MQ=255)
                                          ccATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCac                                               >  1:2846816/1‑93 (MQ=255)
                                           cATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacg                                              <  1:3236018/93‑1 (MQ=255)
                                           cATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacg                                              >  1:2797893/1‑93 (MQ=255)
                                           cATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacg                                              >  1:4327220/1‑93 (MQ=255)
                                           cATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacg                                              >  1:2344149/1‑93 (MQ=255)
                                            aTTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacga                                             <  1:3826690/93‑1 (MQ=255)
                                            aTTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacga                                             <  2:1845040/93‑1 (MQ=255)
                                            aTTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacga                                             <  2:2185218/93‑1 (MQ=255)
                                            aTTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacga                                             <  1:2972914/93‑1 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgac                                            <  1:6039209/93‑1 (MQ=255)
                                              tttGAAGATGCGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgacg                                           <  2:2354717/93‑1 (MQ=255)
                                                tGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacgga                                         <  2:1646449/93‑1 (MQ=255)
                                                tGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacgga                                         <  2:4550725/93‑1 (MQ=255)
                                                  aaGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacggacg                                       >  1:5210395/1‑93 (MQ=255)
                                                  aaGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacggacg                                       >  1:4577854/1‑93 (MQ=255)
                                                   aGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGt                                      >  1:3403527/1‑93 (MQ=255)
                                                   aGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGt                                      <  2:2860674/93‑1 (MQ=255)
                                                    gATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGtt                                     >  1:2107/1‑93 (MQ=255)
                                                     aTACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTa                                    <  2:1059964/93‑1 (MQ=255)
                                                      tACGCGGCGCGTAGTACATGATGATGTTCATACGGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTAc                                   >  2:5281597/1‑93 (MQ=255)
                                                         gcggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGt                                <  2:930823/93‑1 (MQ=255)
                                                         gcggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGt                                >  1:292075/1‑93 (MQ=255)
                                                         gcggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGt                                >  1:4527925/1‑93 (MQ=255)
                                                           ggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTg                              >  1:1811066/1‑93 (MQ=255)
                                                           ggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGCTACGGCTg                              >  2:2921230/1‑93 (MQ=255)
                                                           ggcgCGTAGTACATGATGATGTTCATACCGGCAAACAGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTg                              <  1:810258/93‑1 (MQ=255)
                                                             cgcgTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGAt                            <  2:2537750/93‑1 (MQ=255)
                                                               cgTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCt                          >  1:3559120/1‑93 (MQ=255)
                                                                gtagtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCtt                         >  1:5298782/1‑93 (MQ=255)
                                                                   gtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTaaa                      >  1:3020668/1‑93 (MQ=255)
                                                                     aCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACa                    >  1:5052829/1‑93 (MQ=255)
                                                                       atgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGt                  <  2:1999049/93‑1 (MQ=255)
                                                                       atgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGt                  <  2:1222371/93‑1 (MQ=255)
                                                                        tgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGTACGTTACGGTTGATCTTAAACAGTg                 >  1:475018/1‑93 (MQ=255)
                                                                        tgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTg                 >  1:2871344/1‑93 (MQ=255)
                                                                        tgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTg                 >  2:470613/1‑93 (MQ=255)
                                                                          atgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGcc               <  1:6188025/93‑1 (MQ=255)
                                                                           tgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGccc              <  1:5279165/93‑1 (MQ=255)
                                                                           tgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGccc              >  1:2352712/1‑93 (MQ=255)
                                                                            gatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCa             >  2:4462475/1‑93 (MQ=255)
                                                                            gatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCa             >  2:3200478/1‑93 (MQ=255)
                                                                             atgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCaa            >  2:148197/1‑93 (MQ=255)
                                                                              tgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCCCGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAc           <  1:4351917/93‑1 (MQ=255)
                                                                              tgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAc           <  2:4243383/93‑1 (MQ=255)
                                                                              tgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAc           >  2:4998753/1‑93 (MQ=255)
                                                                              tgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAc           <  2:5864393/93‑1 (MQ=255)
                                                                               gTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAcc          <  2:3575074/93‑1 (MQ=255)
                                                                                ttCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCg         >  1:1062533/1‑93 (MQ=255)
                                                                                   aTACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGccc      <  2:2779301/93‑1 (MQ=255)
                                                                                    tACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCt     <  1:1258777/93‑1 (MQ=255)
                                                                                    tACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCt     <  2:276366/93‑1 (MQ=255)
                                                                                     aCCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTg    <  1:1090348/93‑1 (MQ=255)
                                                                                      ccGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGt   <  2:242989/93‑1 (MQ=255)
                                                                                       cGGCAAACTTCCACATCGCCTGCAACAACAAACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGtt  <  2:743164/93‑1 (MQ=255)
                                                                                       cGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGtt  <  2:917553/93‑1 (MQ=255)
                                                                                       cGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACAGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGtt  <  2:4098211/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT  >  NC_000913/2979301‑2979480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAA  >  NC_000913/2979299‑2979484
                                                                                            |                                                                                             
GACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCA                                                                                              >  SRR4302533.1533586/1‑94 (MQ=60)
 ACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAA                                                                                             >  SRR4302533.4119611/1‑94 (MQ=60)
  CCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAA                                                                                            >  SRR4302533.4371406/1‑94 (MQ=60)
    AGAGTCGCAATCATCTGTTTTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                          <  SRR4302533.1391794/94‑1 (MQ=60)
    AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                          <  SRR4302533.199185/94‑1 (MQ=60)
    AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                          <  SRR4302533.1588833/94‑1 (MQ=60)
     GAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTG                                                                                         <  SRR4302533.2964777/94‑1 (MQ=60)
     GAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTG                                                                                         <  SRR4302533.2365121/94‑1 (MQ=60)
     GAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTG                                                                                         >  SRR4302533.4457507/1‑94 (MQ=60)
      AGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGC                                                                                        >  SRR4302533.2882293/1‑94 (MQ=60)
       GTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCT                                                                                       >  SRR4302533.1183537/1‑94 (MQ=60)
        TCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATATCGGCAAACTGCTG                                                                                      <  SRR4302533.3427366/94‑1 (MQ=60)
        TCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTG                                                                                      <  SRR4302533.3789012/94‑1 (MQ=60)
        TCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTG                                                                                      <  SRR4302533.394942/94‑1 (MQ=60)
          GCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCA                                                                                    >  SRR4302533.1389095/1‑94 (MQ=60)
          GCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCA                                                                                    <  SRR4302533.5944239/94‑1 (MQ=60)
           CAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCAT                                                                                   <  SRR4302533.3863621/94‑1 (MQ=60)
           CAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACAGCTGCAT                                                                                   >  SRR4302533.5963735/1‑94 (MQ=60)
           CAATCATCTGTTGTTCTGTGGGCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCAC                                                                                   >  SRR4302533.5844890/1‑94 (MQ=60)
            AATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATC                                                                                  <  SRR4302533.2942741/94‑1 (MQ=60)
             ATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCG                                                                                 >  SRR4302533.291238/1‑94 (MQ=60)
             ATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCG                                                                                 <  SRR4302533.5204673/94‑1 (MQ=60)
             ATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCG                                                                                 <  SRR4302533.425046/94‑1 (MQ=60)
                ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                              <  SRR4302533.292245/94‑1 (MQ=60)
                ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                              >  SRR4302533.1141539/1‑94 (MQ=60)
                 TCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTG                                                                             <  SRR4302533.3529882/94‑1 (MQ=60)
                 TCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTG                                                                             <  SRR4302533.4854383/94‑1 (MQ=60)
                  CTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGC                                                                            >  SRR4302533.4800530/1‑94 (MQ=60)
                  CTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGGACATGATGATGTTCTTACCGGCAAACTGCTGCATCGCCTGC                                                                            >  SRR4302533.1119713/1‑94 (MQ=60)
                   TGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCA                                                                           <  SRR4302533.527297/94‑1 (MQ=60)
                     TTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAAC                                                                         <  SRR4302533.3966893/94‑1 (MQ=60)
                       GTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAA                                                                       >  SRR4302533.2881513/1‑94 (MQ=60)
                        TTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAAC                                                                      <  SRR4302533.5644006/94‑1 (MQ=60)
                         TCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACA                                                                     <  SRR4302533.678638/94‑1 (MQ=60)
                          CTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAT                                                                    <  SRR4302533.4038044/94‑1 (MQ=60)
                          CTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAT                                                                    <  SRR4302533.1996992/94‑1 (MQ=60)
                          CTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAT                                                                    <  SRR4302533.1676398/94‑1 (MQ=60)
                           TGTGGTCTTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATA                                                                   <  SRR4302533.352524/94‑1 (MQ=60)
                           TGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATA                                                                   <  SRR4302533.1374242/94‑1 (MQ=60)
                           TGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATA                                                                   <  SRR4302533.190312/94‑1 (MQ=60)
                            GTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAC                                                                  >  SRR4302533.5619631/1‑94 (MQ=60)
                             tggcaGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACC                                                                 <  SRR4302533.6019715/89‑1 (MQ=60)
                             TGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACC                                                                 <  SRR4302533.3528052/94‑1 (MQ=60)
                              GGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCG                                                                <  SRR4302533.5436248/94‑1 (MQ=60)
                               GTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGA                                                               >  SRR4302533.2994765/1‑94 (MQ=60)
                                TCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAG                                                              <  SRR4302533.249825/94‑1 (MQ=60)
                                  GTAAAGGCCGCCATTTTGAAGATACGCGGCGCGTAGGACATGATGATGTTCATACCGGCAAACTGCTGCATCGCGTGCAACAAAATACCGAGAA                                                            >  SRR4302533.1899594/1‑94 (MQ=60)
                                  GTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAA                                                            >  SRR4302533.3995760/1‑94 (MQ=60)
                                  GTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAA                                                            >  SRR4302533.2300150/1‑94 (MQ=60)
                                  GTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAA                                                            >  SRR4302533.2292377/1‑94 (MQ=60)
                                   TAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAA                                                           >  SRR4302533.3910815/1‑94 (MQ=60)
                                   TAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAA                                                           <  SRR4302533.5020927/94‑1 (MQ=60)
                                   TAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAA                                                           <  SRR4302533.4088248/94‑1 (MQ=60)
                                     AAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACA                                                         >  SRR4302533.5823376/1‑94 (MQ=60)
                                      AGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACAC                                                        <  SRR4302533.4700047/94‑1 (MQ=60)
                                      AGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACAC                                                        <  SRR4302533.5593356/94‑1 (MQ=60)
                                        CCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAG                                                      <  SRR4302533.926817/94‑1 (MQ=60)
                                        CCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAG                                                      <  SRR4302533.6215283/94‑1 (MQ=60)
                                          CGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCA                                                    >  SRR4302533.3105079/1‑94 (MQ=60)
                                           GCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCAC                                                   >  SRR4302533.2864338/1‑94 (MQ=60)
                                            CCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACG                                                  >  SRR4302533.2358658/1‑94 (MQ=60)
                                            CCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACG                                                  >  SRR4302533.2815102/1‑94 (MQ=60)
                                            CCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACG                                                  >  SRR4302533.4353287/1‑94 (MQ=60)
                                             CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                                 >  SRR4302533.1069618/1‑94 (MQ=60)
                                             CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                                 >  SRR4302533.4194247/1‑94 (MQ=60)
                                             CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                                 >  SRR4302533.2325584/1‑94 (MQ=60)
                                             CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                                 <  SRR4302533.3255958/94‑1 (MQ=60)
                                              ATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGAC                                                <  SRR4302533.3850084/94‑1 (MQ=60)
                                              ATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGAC                                                <  SRR4302533.2991105/94‑1 (MQ=60)
                                               TTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACG                                               <  SRR4302533.5320099/94‑1 (MQ=60)
                                               TTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACG                                               <  SRR4302533.6075805/94‑1 (MQ=60)
                                                 TTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGA                                             >  SRR4302533.4510023/1‑94 (MQ=60)
                                                  TGAAGATACGCGGAGCGTATTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATCCCGAGAAACACAGCACGACGGAC                                            <  SRR4302533.3462518/94‑1 (MQ=60)
                                                   GAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACG                                           >  SRR4302533.4082460/1‑94 (MQ=60)
                                                   GAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACG                                           >  SRR4302533.4605600/1‑94 (MQ=60)
                                                   GAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACG                                           >  SRR4302533.5241780/1‑94 (MQ=60)
                                                    AAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGT                                          >  SRR4302533.3424543/1‑94 (MQ=60)
                                                    AAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGT                                          >  SRR4302533.2901443/1‑94 (MQ=60)
                                                     AGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTT                                         >  SRR4302533.2122/1‑94 (MQ=60)
                                                         ACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGG                                     <  SRR4302533.1420879/94‑1 (MQ=60)
                                                          CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                    <  SRR4302533.1186235/94‑1 (MQ=60)
                                                          CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                    >  SRR4302533.4555346/1‑94 (MQ=60)
                                                          CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                    >  SRR4302533.293859/1‑94 (MQ=60)
                                                          CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                    >  SRR4302533.3112946/1‑94 (MQ=60)
                                                          CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                    <  SRR4302533.3322050/94‑1 (MQ=60)
                                                            TGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTG                                  >  SRR4302533.2145253/1‑94 (MQ=60)
                                                            CGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTG                                  >  SRR4302533.1822130/1‑94 (MQ=60)
                                                             GGCGCGTAGTACATGATGATGTTCATACCGGCAAACAGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGA                                 <  SRR4302533.815012/94‑1 (MQ=60)
                                                                GCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCT                              >  SRR4302533.3581051/1‑94 (MQ=60)
                                                                 CGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTT                             >  SRR4302533.5330527/1‑94 (MQ=60)
                                                                  GTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTA                            >  SRR4302533.5992705/1‑94 (MQ=60)
                                                                   TAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAA                           >  SRR4302533.342566/1‑94 (MQ=60)
                                                                    AGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAA                          >  SRR4302533.3039156/1‑94 (MQ=60)
                                                                      TACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACA                        >  SRR4302533.5083379/1‑94 (MQ=60)
                                                                       ACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAG                       >  SRR4302533.5174558/1‑94 (MQ=60)
                                                                        CATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGT                      <  SRR4302533.5330292/94‑1 (MQ=60)
                                                                         ATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGTACGTTACGGTTGATCTTAAACAGTG                     >  SRR4302533.477855/1‑94 (MQ=60)
                                                                         ATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTG                     >  SRR4302533.2889011/1‑94 (MQ=60)
                                                                         ATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTG                     >  SRR4302533.4664443/1‑94 (MQ=60)
                                                                            ATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCC                  <  SRR4302533.6225164/94‑1 (MQ=60)
                                                                            ATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCC                  >  SRR4302533.3823331/1‑94 (MQ=60)
                                                                            ATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCC                  >  SRR4302533.2367264/1‑94 (MQ=60)
                                                                             TGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCA                 <  SRR4302533.5310806/94‑1 (MQ=60)
                                                                              GATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAA                >  SRR4302533.4254783/1‑94 (MQ=60)
                                                                                TGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCCCGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACC              <  SRR4302533.4378177/94‑1 (MQ=60)
                                                                                TGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACC              >  SRR4302533.2598277/1‑94 (MQ=60)
                                                                                TGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACC              <  SRR4302533.1973021/94‑1 (MQ=60)
                                                                                TGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACC              <  SRR4302533.4594155/94‑1 (MQ=60)
                                                                                 GTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCG             >  SRR4302533.1068777/1‑94 (MQ=60)
                                                                                   TCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCC           >  SRR4302533.588986/1‑94 (MQ=60)
                                                                                    CATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCC          >  SRR4302533.2829927/1‑94 (MQ=60)
                                                                                      TACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTG        <  SRR4302533.1266305/94‑1 (MQ=60)
                                                                                      TACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTG        >  SRR4302533.5374718/1‑94 (MQ=60)
                                                                                       ACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGT       <  SRR4302533.1096791/94‑1 (MQ=60)
                                                                                        CCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT      <  SRR4302533.1875029/94‑1 (MQ=60)
                                                                                        CCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT      >  SRR4302533.4012632/1‑94 (MQ=60)
                                                                                         CGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTT     >  SRR4302533.6183925/1‑94 (MQ=60)
                                                                                          GGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCACTGTTTT    >  SRR4302533.1503468/1‑94 (MQ=60)
                                                                                           GCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTA   >  SRR4302533.3435115/1‑94 (MQ=60)
                                                                                           GCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTA   <  SRR4302533.3988960/94‑1 (MQ=60)
                                                                                            CAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAA  <  SRR4302533.1761130/94‑1 (MQ=60)
                                                                                            CAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAA  >  SRR4302533.5962997/1‑94 (MQ=60)
                                                                                            CAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAA  >  SRR4302533.4758782/1‑94 (MQ=60)
                                                                                            |                                                                                             
GACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAA  >  NC_000913/2979299‑2979484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑