Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,001,717	+C	coding (86/903 nt)	rarD ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,001,717	1	.	C	100.0%	667.9 / NA	212	T29R (ACG→AGG)	rarD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (80/132); total (80/132)

Only 100 of 252 total aligned reads displayed.

TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT  >  NC_000913/4001625‑4001802

tGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACg                                                                                        >  1:2175927/1‑93 (MQ=255)

 gCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgc                                                                                       >  2:3326783/1‑92 (MQ=255)

  cTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgct                                                                                      >  2:2824202/1‑91 (MQ=255)

  cTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgct                                                                                      <  1:1341771/93‑3 (MQ=255)

  cTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACgct                                                                                      >  1:5465104/1‑91 (MQ=255)

   tcatcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgctg                                                                                     >  1:3945805/1‑90 (MQ=255)

   tcatcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGAACgctg                                                                                     >  1:4882795/1‑90 (MQ=255)

    catcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgctgg                                                                                    >  1:4076158/1‑89 (MQ=255)

        aGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCt                                                                                <  1:6244454/93‑1 (MQ=255)

          caccacCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCtat                                                                              <  1:600101/93‑1 (MQ=255)

           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAATTTGAAGTACGCTGGCGCtata                                                                             <  2:4008949/93‑1 (MQ=255)

            ccaccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAc                                                                            >  2:4559731/1‑93 (MQ=255)

            ccaccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAc                                                                            <  2:1601262/93‑1 (MQ=255)

              accaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAccc                                                                          <  2:1589871/93‑1 (MQ=255)

              accaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAccc                                                                          >  1:5884042/1‑93 (MQ=255)

                caTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTCGTCAATCAACTTGAAGCAGGCTGGCGCTATACCCCa                                                                        >  1:5261649/1‑93 (MQ=255)

                caTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACGCTGGCGCTATACCCCa                                                                        >  2:2071528/1‑93 (MQ=255)

                 aTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaa                                                                       >  2:2239886/1‑93 (MQ=255)

                  tAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACGCTGGCGCTATACCCCaaa                                                                      >  2:883195/1‑93 (MQ=255)

                    aaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaata                                                                    >  1:2930120/1‑93 (MQ=255)

                       aaaaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataaaa                                                                 <  2:4349163/93‑1 (MQ=255)

                           aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAg                                                             <  1:3747335/93‑1 (MQ=255)

                           aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAg                                                             <  1:596674/93‑1 (MQ=255)

                            cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGc                                                            <  1:2623905/93‑1 (MQ=255)

                            cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGc                                                            <  1:3182348/93‑1 (MQ=255)

                            cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGc                                                            <  1:4175820/93‑1 (MQ=255)

                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCg                                                           <  1:4769510/93‑1 (MQ=255)

                             caCCAGCTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCg                                                           <  2:2875549/92‑1 (MQ=255)

                              aCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGTAGTAAATCAACTTGAAGGACGCTTGCGCTATACCCCAAATAAAATAAgatg                                                          >  2:1143691/1‑90 (MQ=255)

                              aCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAAGCTGGCGCTATACCCCAAATAAAAAAAGAgg                                                          >  1:880211/1‑93 (MQ=255)

                               ccAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGc                                                         <  2:1263059/93‑1 (MQ=255)

                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                       <  1:4574604/93‑1 (MQ=255)

                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                       >  1:6131571/1‑93 (MQ=255)

                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                       <  1:296887/93‑1 (MQ=255)

                                  gATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAag                                                      <  2:745848/93‑1 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                     <  1:4385216/93‑1 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                     >  1:1677336/1‑93 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                     <  2:5156459/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                    <  2:6028138/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                    <  1:3852176/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                    <  2:1517323/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                    <  2:2321566/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                    <  1:5229805/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGAACGCTGGCGCTATACCCCAAATAAAATAAGCGGCCagag                                                    >  1:1111880/1‑93 (MQ=255)

                                      aCGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCg                                                  >  2:4790543/1‑93 (MQ=255)

                                      aCGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCg                                                  <  2:3405366/93‑1 (MQ=255)

                                      aCGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCg                                                  >  2:2713790/1‑93 (MQ=255)

                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGa                                                 <  1:1185854/93‑1 (MQ=255)

                                        gcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAg                                                <  2:4760080/93‑1 (MQ=255)

                                         cgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATTAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAACGGCAAGAGCGAGt                                               >  2:5367409/1‑93 (MQ=255)

                                         cgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGt                                               <  2:5516121/93‑1 (MQ=255)

                                         cgATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAga                                               >  1:3316467/1‑92 (MQ=255)

                                           aTGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTaa                                             <  2:5956425/93‑1 (MQ=255)

                                           aTGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTaa                                             >  1:4990858/1‑93 (MQ=255)

                                             gCGTCAGGATTTCATCGGCGGGCACGTAGTAAAGCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATa                                           <  1:3985786/93‑1 (MQ=255)

                                              cGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAc                                          <  2:5423424/93‑1 (MQ=255)

                                               gTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACg                                         >  1:5622932/1‑93 (MQ=255)

                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGc                                        <  2:4602204/93‑1 (MQ=255)

                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGc                                        <  2:5894619/93‑1 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                      >  2:4456612/1‑93 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                      >  2:239511/1‑93 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                      <  1:5703704/93‑1 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                      <  2:3112108/93‑1 (MQ=255)

                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                     <  2:3541685/93‑1 (MQ=255)

                                                    gATTTCATCGGCGGGGACGTAGTAAATCAACCTGGAGTACGCTGGGGGTATACCCCAAATAAAATAAACGGCAAGAGCGAGCAATACGCCCTg                                    >  2:3668330/1‑93 (MQ=255)

                                                     aTTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGc                                   <  1:509286/93‑1 (MQ=255)

                                                     aTTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGc                                   <  2:4458950/93‑1 (MQ=255)

                                                      tttCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGcc                                  >  2:3459155/1‑93 (MQ=255)

                                                          aTCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                              <  2:5471307/93‑1 (MQ=255)

                                                           tCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                             <  2:4366726/93‑1 (MQ=255)

                                                           tCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                             <  2:2550856/93‑1 (MQ=255)

                                                             ggcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttg                           >  1:2742820/1‑93 (MQ=255)

                                                             ggcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttg                           >  1:1000585/1‑93 (MQ=255)

                                                              gcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt                          <  2:6221888/93‑1 (MQ=255)

                                                                gggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgttt                        <  2:2424507/93‑1 (MQ=255)

                                                                gggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgttt                        <  1:1566877/93‑1 (MQ=255)

                                                                 ggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGtttt                       <  2:137959/93‑1 (MQ=255)

                                                                 ggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGtttt                       >  2:3968062/1‑93 (MQ=255)

                                                                   cACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGc                     <  2:951136/93‑1 (MQ=255)

                                                                   cACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGc                     >  1:5910080/1‑93 (MQ=255)

                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                    <  1:3619060/93‑1 (MQ=255)

                                                                       tagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcc                 >  2:2955843/1‑93 (MQ=255)

                                                                        agtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                >  2:5704627/1‑93 (MQ=255)

                                                                        agtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                <  1:5495114/93‑1 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat               >  2:4175933/1‑93 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat               <  2:6255785/93‑1 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat               <  1:2314409/93‑1 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat               >  1:4633673/1‑93 (MQ=255)

                                                                          taAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc              >  2:2473377/1‑93 (MQ=255)

                                                                           aaaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg             >  1:761367/1‑93 (MQ=255)

                                                                           aaaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg             >  1:3005048/1‑93 (MQ=255)

                                                                           aaaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg             >  2:2019096/1‑93 (MQ=255)

                                                                           aaaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg             >  1:1969386/1‑93 (MQ=255)

                                                                            aaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATACATCgg            <  1:1776864/93‑1 (MQ=255)

                                                                              tCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGa          <  1:3423242/93‑1 (MQ=255)

                                                                                aaCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAt        <  2:5137965/93‑1 (MQ=255)

                                                                                 aCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATa       <  2:1310191/93‑1 (MQ=255)

                                                                                    tGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTc    <  1:1859331/93‑1 (MQ=255)

                                                                                     gAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCa   <  1:1051849/93‑1 (MQ=255)

                                                                                      aaGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt  <  1:4399208/93‑1 (MQ=255)

TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT  >  NC_000913/4001625‑4001802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  NC_000913/4001624‑4001810

ATGCTCATCAGCACCACCATAAAGAAAAACGGCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G                                                                                                <  SRR4302533.4086338/94‑1 (MQ=60)

ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G                                                                                                >  SRR4302533.2189339/1‑94 (MQ=60)

  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTAC‑Gct                                                                                              >  SRR4302533.5498038/1‑92 (MQ=60)

   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctg                                                                                             >  SRR4302533.3969868/1‑91 (MQ=60)

   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctg                                                                                             <  SRR4302533.2765618/94‑4 (MQ=60)

   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctg                                                                                             <  SRR4302533.1349850/94‑4 (MQ=60)

    TCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGG                                                                                            >  SRR4302533.4100970/1‑94 (MQ=60)

     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGC                                                                                           <  SRR4302533.4034443/94‑1 (MQ=60)

     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGC                                                                                           <  SRR4302533.399950/94‑1 (MQ=60)

     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGC                                                                                           >  SRR4302533.1969551/1‑94 (MQ=60)

         AGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTA                                                                                       <  SRR4302533.6281831/94‑1 (MQ=60)

          GCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTAT                                                                                      <  SRR4302533.103730/94‑1 (MQ=60)

           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATA                                                                                     >  SRR4302533.3324907/1‑94 (MQ=60)

           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATA                                                                                     >  SRR4302533.6128526/1‑94 (MQ=60)

           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATA                                                                                     <  SRR4302533.603631/94‑1 (MQ=60)

            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAC                                                                                    <  SRR4302533.3015803/94‑1 (MQ=60)

            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAC                                                                                    <  SRR4302533.5410862/94‑1 (MQ=60)

            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAC                                                                                    >  SRR4302533.633141/1‑94 (MQ=60)

            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAC                                                                                    >  SRR4302533.597158/1‑94 (MQ=60)

            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTAC‑GCGGGCGCTATAC                                                                                    >  SRR4302533.496883/1‑94 (MQ=60)

             CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACC                                                                                   >  SRR4302533.3968911/1‑94 (MQ=60)

             CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACC                                                                                   >  SRR4302533.4796510/1‑94 (MQ=60)

              CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCC                                                                                  >  SRR4302533.5919601/1‑94 (MQ=60)

              CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCC                                                                                  <  SRR4302533.5039462/94‑1 (MQ=60)

              CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCC                                                                                  <  SRR4302533.4146160/94‑1 (MQ=60)

               ACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCC                                                                                 <  SRR4302533.1650079/94‑1 (MQ=60)

                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTCGTCAATCAACTTGAAGCAG‑GCTGGCGCTATACCCCA                                                                                >  SRR4302533.5293237/1‑94 (MQ=60)

                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCA                                                                                <  SRR4302533.3983140/94‑1 (MQ=60)

                 CATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCCCGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCCA                                                                               >  SRR4302533.676183/1‑94 (MQ=60)

                   TAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAAT                                                                             <  SRR4302533.5199785/94‑1 (MQ=60)

                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATA                                                                            <  SRR4302533.5359607/94‑1 (MQ=60)

                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATA                                                                            >  SRR4302533.2948042/1‑94 (MQ=60)

                     AAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAA                                                                           <  SRR4302533.1285391/94‑1 (MQ=60)

                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAA                                                                          <  SRR4302533.4762095/94‑1 (MQ=60)

                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAA                                                                          >  SRR4302533.3204690/1‑94 (MQ=60)

                        AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAAT                                                                        <  SRR4302533.5694178/94‑1 (MQ=60)

                            ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGC                                                                    <  SRR4302533.3770311/94‑1 (MQ=60)

                            ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGC                                                                    <  SRR4302533.600192/94‑1 (MQ=60)

                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCG                                                                   <  SRR4302533.3201926/94‑1 (MQ=60)

                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCG                                                                   <  SRR4302533.2640010/94‑1 (MQ=60)

                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCG                                                                   >  SRR4302533.2623623/1‑94 (MQ=60)

                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCG                                                                   <  SRR4302533.4201122/94‑1 (MQ=60)

                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGG                                                                  >  SRR4302533.1077044/1‑94 (MQ=60)

                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGG                                                                  <  SRR4302533.4798419/94‑1 (MQ=60)

                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGG                                                                  >  SRR4302533.1605691/1‑94 (MQ=60)

                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGAGG                                                                  >  SRR4302533.3193874/1‑94 (MQ=60)

                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCCATACCCCCAATAAAAAAAGCGG                                                                  >  SRR4302533.5645321/1‑94 (MQ=60)

                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAA‑GCTGGCGCTATACCCCAAATAAAAAAAGAGG                                                                  >  SRR4302533.885380/1‑94 (MQ=60)

                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAA                                                               >  SRR4302533.6168515/1‑94 (MQ=60)

                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302533.298705/94‑1 (MQ=60)

                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302533.300311/94‑1 (MQ=60)

                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302533.5941249/94‑1 (MQ=60)

                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302533.4602328/94‑1 (MQ=60)

                                   GATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGA                                                             >  SRR4302533.1687492/1‑94 (MQ=60)

                                   GATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAACGGCAAGA                                                             >  SRR4302533.1242505/1‑94 (MQ=60)

                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302533.4411694/94‑1 (MQ=60)

                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302533.1552101/94‑1 (MQ=60)

                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGCGTAAATCAACTTGAAATAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAC                                                            >  SRR4302533.6236453/1‑94 (MQ=60)

                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGAAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCCAGAG                                                            >  SRR4302533.1118479/1‑94 (MQ=60)

                                     TCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGC                                                           <  SRR4302533.3875701/94‑1 (MQ=60)

                                     TCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGC                                                           <  SRR4302533.5261265/94‑1 (MQ=60)

                                      CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCG                                                          <  SRR4302533.4822546/94‑1 (MQ=60)

                                        CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                        <  SRR4302533.1192971/94‑1 (MQ=60)

                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGA                                                       >  SRR4302533.4928645/1‑94 (MQ=60)

                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGA                                                       >  SRR4302533.3336948/1‑94 (MQ=60)

                                          CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTA                                                      >  SRR4302533.6046326/1‑94 (MQ=60)

                                           GATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAA                                                     >  SRR4302533.5021117/1‑94 (MQ=60)

                                            ATGCGTCAGGATTTCATCGGCGGGCACGTGGTAAATTAACTTGAAGTACGG‑TGGAGCTCTACCCCAAATAAAATAAGGCGGAAGAGCGAGCAAT                                                    >  SRR4302533.1349399/1‑94 (MQ=53)

                                             TGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA                                                   <  SRR4302533.348609/94‑1 (MQ=60)

                                             TGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA                                                   >  SRR4302533.3800992/1‑94 (MQ=60)

                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302533.3382647/94‑1 (MQ=60)

                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302533.1169520/94‑1 (MQ=60)

                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302533.2090596/94‑1 (MQ=60)

                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302533.4400955/94‑1 (MQ=60)

                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAAGCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302533.4010060/94‑1 (MQ=60)

                                               CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACG                                                 >  SRR4302533.5656917/1‑94 (MQ=60)

                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGGAATACGC                                                >  SRR4302533.564145/1‑94 (MQ=60)

                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             <  SRR4302533.5738157/94‑1 (MQ=60)

                                                    GGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTG                                            <  SRR4302533.5519300/94‑1 (MQ=60)

                                                      ATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCC                                          <  SRR4302533.512311/94‑1 (MQ=60)

                                                        TTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGC                                        >  SRR4302533.909311/1‑94 (MQ=60)

                                                         TCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCG                                       <  SRR4302533.838288/94‑1 (MQ=60)

                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                                   >  SRR4302533.1006425/1‑94 (MQ=60)

                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                                   >  SRR4302533.2759637/1‑94 (MQ=60)

                                                               GCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                                 <  SRR4302533.4420956/94‑1 (MQ=60)

                                                               GCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                                 <  SRR4302533.3820551/94‑1 (MQ=60)

                                                               GCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGCAATACGCCCTGCCGCGTTTGTT                                 >  SRR4302533.1062435/1‑94 (MQ=60)

                                                                 GGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTT                               >  SRR4302533.251120/1‑94 (MQ=60)

                                                                 GGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTT                               <  SRR4302533.1576311/94‑1 (MQ=60)

                                                                  GGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTG                              <  SRR4302533.4584011/94‑1 (MQ=60)

                                                                  GGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTG                              <  SRR4302533.2716026/94‑1 (MQ=60)

                                                                   GCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGC                             >  SRR4302533.5945782/1‑94 (MQ=60)

                                                                    CACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                            >  SRR4302533.1805966/1‑94 (MQ=60)

                                                                     ACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302533.1950104/94‑1 (MQ=60)

                                                                     ACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302533.3641321/94‑1 (MQ=60)

                                                                      CGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                          >  SRR4302533.5782309/1‑94 (MQ=60)

                                                                      CGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                          <  SRR4302533.5201950/94‑1 (MQ=60)

                                                                      CGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                          >  SRR4302533.2971419/1‑94 (MQ=60)

                                                                      CGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                          <  SRR4302533.3662236/94‑1 (MQ=60)

                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                         <  SRR4302533.2074700/94‑1 (MQ=60)

                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                         >  SRR4302533.4615703/1‑94 (MQ=60)

                                                                        TAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302533.5291568/94‑1 (MQ=60)

                                                                         AGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       <  SRR4302533.5528261/94‑1 (MQ=60)

                                                                         AGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       >  SRR4302533.4661751/1‑94 (MQ=60)

                                                                          GTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                      <  SRR4302533.2328746/94‑1 (MQ=60)

                                                                          GTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                      <  SRR4302533.1851591/94‑1 (MQ=60)

                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     >  SRR4302533.3023429/1‑94 (MQ=60)

                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     >  SRR4302533.765849/1‑94 (MQ=60)

                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     >  SRR4302533.1981430/1‑94 (MQ=60)

                                                                            AAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                    <  SRR4302533.5958244/94‑1 (MQ=60)

                                                                             AATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATACATCGGG                   <  SRR4302533.1787681/94‑1 (MQ=60)

                                                                               TCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAA                 <  SRR4302533.3444387/94‑1 (MQ=60)

                                                                                 AACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA               <  SRR4302533.5907468/94‑1 (MQ=60)

                                                                                 AACTTGAAGTAC‑GCTGGCGCTATACCACAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA               >  SRR4302533.6012298/1‑94 (MQ=60)

                                                                                  ACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAggacggggaaaaaggccctgcccggtttgttttgcttccatcgggattat              >  SRR4302533.4066387/1‑44 (MQ=60)

                                                                                  ACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAC              >  SRR4302533.3516390/1‑94 (MQ=60)

                                                                                   CTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT             >  SRR4302533.1750315/1‑94 (MQ=60)

                                                                                    TTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTC            <  SRR4302533.2923614/94‑1 (MQ=60)

                                                                                     TGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCA           <  SRR4302533.1870672/94‑1 (MQ=60)

                                                                                     TGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCA           >  SRR4302533.2290942/1‑94 (MQ=60)

                                                                                      GAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT          <  SRR4302533.1058041/94‑1 (MQ=60)

                                                                                      GAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGGTTGTTTTGCATCCATCGGGAATACTCAT          >  SRR4302533.4349933/1‑94 (MQ=60)

                                                                                       AAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT         <  SRR4302533.4470575/94‑1 (MQ=60)

                                                                                       AAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT         <  SRR4302533.4425768/94‑1 (MQ=60)

                                                                                        AGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTT        >  SRR4302533.5063503/1‑94 (MQ=60)

                                                                                        AGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTT        >  SRR4302533.5063504/1‑94 (MQ=60)

                                                                                         GTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       <  SRR4302533.1226906/94‑1 (MQ=60)

                                                                                         GTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       >  SRR4302533.2204540/1‑94 (MQ=60)

                                                                                         GTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       >  SRR4302533.4902754/1‑94 (MQ=60)

                                                                                         GTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       >  SRR4302533.4435137/1‑94 (MQ=60)

                                                                                              C‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  SRR4302533.3197041/1‑94 (MQ=60)

ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  NC_000913/4001624‑4001810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`