Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,227,795	A→G	intergenic (‑74/‑138)	pepE ← / → yjbC	peptidase E/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,227,795	0	A	G	100.0%	771.1 / NA	242	intergenic (‑74/‑138)	pepE/yjbC	peptidase E/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (135/107); total (135/107)

Only 100 of 269 total aligned reads displayed.

AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT  >  NC_000913/4227705‑4227884

aaTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTcgcg                                                                                         >  2:1348667/1‑93 (MQ=255)

aaTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTcgcg                                                                                         >  1:2739272/1‑93 (MQ=255)

aaTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTcgcg                                                                                         >  1:6180458/1‑93 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAt                                                                                       >  2:3800349/1‑93 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAt                                                                                       >  1:4191900/1‑93 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAt                                                                                       <  1:36790/93‑1 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACC‑CTGCGAATTTAACCACTTACTGACATGGCTCGCGGTg                                                                                      >  2:5869779/1‑93 (MQ=255)

   aaaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATg                                                                                      <  1:5552547/93‑1 (MQ=255)

    aaaGCAGTTCCATTACTTCTCCGGTGAGTAAGAAAGCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGt                                                                                     <  1:1679654/93‑1 (MQ=255)

     aaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTc                                                                                    >  1:4020937/1‑93 (MQ=255)

     aaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTc                                                                                    >  2:5505022/1‑93 (MQ=255)

       gCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGc                                                                                  >  1:4635304/1‑93 (MQ=255)

         aGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCgtcc                                                                                >  2:3110341/1‑90 (MQ=255)

          gTTCCTTTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGGGATGTCGcaca                                                                               >  1:5221847/1‑93 (MQ=255)

          gTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGGTGTAGcaca                                                                               >  1:5137039/1‑93 (MQ=255)

          gTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTGGccca                                                                               >  1:4316549/1‑93 (MQ=255)

          gTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGcaca                                                                               >  2:701524/1‑93 (MQ=255)

           ttCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAt                                                                              >  2:3998563/1‑93 (MQ=255)

           ttCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAt                                                                              <  1:760856/93‑1 (MQ=255)

            tCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAtt                                                                             <  2:3321624/93‑1 (MQ=255)

              cATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGc                                                                           >  1:1926359/1‑93 (MQ=255)

               aTTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCt                                                                          <  2:320051/93‑1 (MQ=255)

                 tACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCTATGTCGCACATTGCTTc                                                                        <  2:4192810/93‑1 (MQ=255)

                 tACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTc                                                                        >  2:47064/1‑93 (MQ=255)

                  aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCt                                                                       >  2:1836442/1‑93 (MQ=255)

                   cTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTg                                                                      <  2:5187159/93‑1 (MQ=255)

                    ttCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTgg                                                                     >  2:6259212/1‑93 (MQ=255)

                    ttCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGGCGCACATTGCCTCTgg                                                                     >  1:3094120/1‑93 (MQ=255)

                     tctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGa                                                                    >  2:4254139/1‑93 (MQ=255)

                     tctcCCGTTAGTAAGAAATCACGGGAGGCATGCTACTACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGa                                                                    <  1:641061/93‑1 (MQ=255)

                      ctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTggga                                                                   >  1:5304581/1‑91 (MQ=255)

                       tcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGaaa                                                                  >  2:4438094/1‑93 (MQ=255)

                       tcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGaaa                                                                  >  2:1704624/1‑93 (MQ=255)

                           gTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGa                                                              <  2:137985/93‑1 (MQ=255)

                           gTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGa                                                              <  1:4108061/93‑1 (MQ=255)

                           gTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGa                                                              >  1:4441911/1‑93 (MQ=255)

                           gTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGa                                                              >  1:1810909/1‑93 (MQ=255)

                            ttAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAt                                                             >  1:5921268/1‑93 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCTAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           <  1:2226572/93‑1 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           <  2:895404/93‑1 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           <  2:2336485/93‑1 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           >  2:5285009/1‑93 (MQ=255)

                               gTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTg                                                          >  2:4596614/1‑93 (MQ=255)

                               gTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAATGCTTCTGGGAAGTGATTTg                                                          >  1:5658473/1‑93 (MQ=255)

                                tAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGa                                                         >  1:4884830/1‑93 (MQ=255)

                                 aaGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAg                                                        <  1:2509564/93‑1 (MQ=255)

                                 aaGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAg                                                        <  1:237804/93‑1 (MQ=255)

                                 aaGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAg                                                        >  2:4132085/1‑93 (MQ=255)

                                  aGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGc                                                       <  1:3124303/93‑1 (MQ=255)

                                  aGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGc                                                       <  2:1036719/93‑1 (MQ=255)

                                    aaaTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGTCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAg                                                     <  2:5536123/93‑1 (MQ=255)

                                    aaaTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAg                                                     <  1:1378954/93‑1 (MQ=255)

                                    aaaTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAg                                                     <  2:6080312/93‑1 (MQ=255)

                                      aTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGt                                                   >  2:53157/1‑93 (MQ=255)

                                       tCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTa                                                  >  1:1325798/1‑93 (MQ=255)

                                        cACGGGAGGCATGCTGCCACTGCGAATTTAACCACCTGCTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTaa                                                 <  2:5219039/93‑1 (MQ=255)

                                         aCGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAc                                                <  2:5665978/93‑1 (MQ=255)

                                            ggAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGTAACGaa                                             >  1:1860345/1‑93 (MQ=255)

                                               ggCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTc                                          <  2:5658473/93‑1 (MQ=255)

                                               ggCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTc                                          >  2:4562484/1‑93 (MQ=255)

                                                gCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCa                                         <  1:1510987/93‑1 (MQ=255)

                                                  aTGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAg                                       <  1:4409031/93‑1 (MQ=255)

                                                  aTGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAg                                       >  2:5067556/1‑93 (MQ=255)

                                                  aTGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAg                                       >  1:3603765/1‑93 (MQ=255)

                                                   tGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAAGAATTCAAGc                                      >  1:92484/1‑93 (MQ=255)

                                                   tGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTCGAAAGTGATTTGAGCAGGTAACGAATTCAAGc                                      >  1:3773556/1‑93 (MQ=255)

                                                    gCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGcc                                     <  1:603370/93‑1 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   >  1:3366600/1‑93 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   >  2:2185205/1‑93 (MQ=255)

                                                      tACCACTGCAAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   <  2:1425916/93‑1 (MQ=255)

                                                       aCCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCCCATTGCTTCTGGAAAGTGATTTGAGCAGGTAAAGAATTCAAGCCAAt                                  >  2:2823202/1‑93 (MQ=255)

                                                         cACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATgg                                >  2:4914100/1‑93 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGa                               <  1:723976/93‑1 (MQ=255)

                                                            tGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGaaa                             >  1:301113/1‑93 (MQ=255)

                                                              cGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATa                           <  1:2279362/93‑1 (MQ=255)

                                                               gAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATaa                          >  1:6147713/1‑93 (MQ=255)

                                                               gAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATaa                          >  2:2733750/1‑93 (MQ=255)

                                                               gAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAATGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATaa                          >  1:1544735/1‑93 (MQ=255)

                                                                 aTTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGGAACGAATTCAAGCCAATGGAAATAACa                        >  1:478533/1‑93 (MQ=255)

                                                                  tttAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACaa                       >  1:5003447/1‑93 (MQ=255)

                                                                   ttAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACaaa                      <  1:5368246/93‑1 (MQ=255)

                                                                    tAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAt                     <  2:2995365/93‑1 (MQ=255)

                                                                     aaCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATa                    <  2:2650241/93‑1 (MQ=255)

                                                                      aCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAg                   >  2:5439033/1‑93 (MQ=255)

                                                                        cACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 >  2:1758267/1‑93 (MQ=255)

                                                                        cACTTACTGACATGGCTCGCGATATCACACATTGCTTCTGGGAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 >  2:25911/1‑93 (MQ=255)

                                                                        cACTTACTGACATGGCTCGAGAGGTCGCACATTGCTTGTGAAAGGTGATTAGAGCAAGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 <  2:1121561/93‑1 (MQ=255)

                                                                           ttACTGACATGGCTCGCGATGTCGCACATTGCTTCTGAAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGc              <  1:2603290/93‑1 (MQ=255)

                                                                            tACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCt             >  1:5072996/1‑93 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAt          >  1:4066538/1‑93 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAt          >  2:2926682/1‑93 (MQ=255)

                                                                                 aCATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGa        >  1:3441192/1‑93 (MQ=255)

                                                                                  cATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGAc       >  1:4531995/1‑93 (MQ=255)

                                                                                   aTGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACt      >  2:1209957/1‑93 (MQ=255)

                                                                                   aTGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACt      >  1:2072039/1‑93 (MQ=255)

                                                                                     ggCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAt    <  1:3317766/93‑1 (MQ=255)

                                                                                     ggCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAt    >  2:3624202/1‑93 (MQ=255)

                                                                                     ggCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAt    >  2:1584399/1‑93 (MQ=255)

                                                                                     ggCTCGCGATGGCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGGAACGAATTCAAGCCCATGGAAATAACAAATAGTTCGCTCATGACTAt    >  2:2408484/1‑93 (MQ=255)

                                                                                       cTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAttt  <  2:5160801/93‑1 (MQ=255)

AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT  >  NC_000913/4227705‑4227884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTTA  >  NC_000913/4227702‑4227888

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCG                                                                                               <  SRR4302533.509994/94‑1 (MQ=60)

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCG                                                                                               <  SRR4302533.1703070/94‑1 (MQ=60)

  CAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCG                                                                                             >  SRR4302533.2756069/1‑94 (MQ=60)

  CAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCG                                                                                             >  SRR4302533.6217576/1‑94 (MQ=60)

   AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGA                                                                                            >  SRR4302533.2294164/1‑94 (MQ=60)

   AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGA                                                                                            <  SRR4302533.2592526/94‑1 (MQ=60)

   AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTCAACCACTTACTGACATGGCTCGCGA                                                                                            >  SRR4302533.3527027/1‑94 (MQ=60)

    ATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAT                                                                                           >  SRR4302533.4217270/1‑94 (MQ=60)

     TAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATG                                                                                          <  SRR4302533.37032/94‑1 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                         <  SRR4302533.5586079/94‑1 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                         >  SRR4302533.1544337/1‑94 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                        >  SRR4302533.4507612/1‑94 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                        <  SRR4302533.6184584/94‑1 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                        <  SRR4302533.3756772/94‑1 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                        >  SRR4302533.4045423/1‑94 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                        >  SRR4302533.4485334/1‑94 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTGAGTAAGAAAGCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                        <  SRR4302533.1689828/94‑1 (MQ=60)

         AGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGC                                                                                      >  SRR4302533.4663390/1‑94 (MQ=60)

            AGTTCCTTTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGGGATGTCGCACA                                                                                   >  SRR4302533.5253268/1‑94 (MQ=60)

            AGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGGTGTAGCACA                                                                                   >  SRR4302533.5168080/1‑94 (MQ=60)

            AGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTGGCCCA                                                                                   >  SRR4302533.4342542/1‑94 (MQ=60)

             GTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAT                                                                                  <  SRR4302533.324464/94‑1 (MQ=60)

             GTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAT                                                                                  <  SRR4302533.1913334/94‑1 (MQ=60)

             GTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAT                                                                                  <  SRR4302533.3060796/94‑1 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                                 >  SRR4302533.186258/1‑94 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                                 <  SRR4302533.765333/94‑1 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                                 <  SRR4302533.6036134/94‑1 (MQ=60)

               TCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTG                                                                                <  SRR4302533.183632/94‑1 (MQ=60)

                CCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGC                                                                               >  SRR4302533.1938112/1‑94 (MQ=60)

                 CATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCT                                                                              >  SRR4302533.3973064/1‑94 (MQ=60)

                  ATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTT                                                                             >  SRR4302533.4944402/1‑94 (MQ=60)

                    TACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCT                                                                           <  SRR4302533.2490503/94‑1 (MQ=60)

                     TCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTG                                                                          <  SRR4302533.1985094/94‑1 (MQ=60)

                     ACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTG                                                                          >  SRR4302533.2273513/1‑94 (MQ=60)

                      CTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTTG                                                                         >  SRR4302533.1532192/1‑94 (MQ=60)

                      CTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGGCGCACATTGCCTCTGG                                                                         >  SRR4302533.3113143/1‑94 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                        >  SRR4302533.3255271/1‑94 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                        <  SRR4302533.5966148/94‑1 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                        <  SRR4302533.5234593/94‑1 (MQ=60)

                        TCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGA                                                                       >  SRR4302533.5336368/1‑94 (MQ=60)

                        TCTCCCGTTAGTAAGAAATCACGGGAGGCATGCTACTACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAA                                                                       <  SRR4302533.644830/94‑1 (MQ=60)

                         CTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGACA                                                                      >  SRR4302533.4356786/1‑94 (MQ=60)

                         CTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAA                                                                      >  SRR4302533.590041/1‑94 (MQ=60)

                         CTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAA                                                                      <  SRR4302533.780620/94‑1 (MQ=60)

                           CCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGT                                                                    >  SRR4302533.1466881/1‑94 (MQ=60)

                             GGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGA                                                                  >  SRR4302533.1821973/1‑94 (MQ=60)

                             GGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGA                                                                  >  SRR4302533.4468769/1‑94 (MQ=60)

                              GTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAT                                                                 <  SRR4302533.4133037/94‑1 (MQ=60)

                              GTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAT                                                                 >  SRR4302533.5957061/1‑94 (MQ=60)

                               TTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATT                                                                <  SRR4302533.4212448/94‑1 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCTAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTG                                                              <  SRR4302533.2240317/94‑1 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAATGCTTCTGGGAAGTGATTTG                                                              >  SRR4302533.5692665/1‑94 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGA                                                             >  SRR4302533.4914424/1‑94 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGA                                                             >  SRR4302533.2706404/1‑94 (MQ=60)

                                    AAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGC                                                           <  SRR4302533.239276/94‑1 (MQ=60)

                                    AAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGC                                                           >  SRR4302533.4885294/1‑94 (MQ=60)

                                    AAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGC                                                           <  SRR4302533.2525026/94‑1 (MQ=60)

                                    AAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGGCGCCCATTGCCTCTGGAAAGTGATTTtacc                                                           >  SRR4302533.510679/1‑90 (MQ=60)

                                     AGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCA                                                          <  SRR4302533.3143508/94‑1 (MQ=60)

                                      GAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAG                                                         <  SRR4302533.763381/94‑1 (MQ=60)

                                       AAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGG                                                        <  SRR4302533.642969/94‑1 (MQ=60)

                                       AAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGG                                                        <  SRR4302533.1387250/94‑1 (MQ=60)

                                        AATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGT                                                       <  SRR4302533.4144062/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                      <  SRR4302533.5886406/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                      >  SRR4302533.1333750/1‑94 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                      <  SRR4302533.3370952/94‑1 (MQ=60)

                                          TCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAA                                                     <  SRR4302533.5044311/94‑1 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCACACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACG                                                   <  SRR4302533.5222384/94‑1 (MQ=60)

                                             CGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGA                                                  >  SRR4302533.6161108/1‑94 (MQ=60)

                                              GGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGTAACGAA                                                 >  SRR4302533.1871691/1‑94 (MQ=60)

                                               GGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCCATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAAT                                                >  SRR4302533.1447817/1‑94 (MQ=60)

                                                 AGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTC                                              >  SRR4302533.5346265/1‑94 (MQ=60)

                                                  GGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCA                                             <  SRR4302533.3131174/94‑1 (MQ=60)

                                                  GGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCA                                             <  SRR4302533.1729450/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                            <  SRR4302533.698581/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                            <  SRR4302533.1520093/94‑1 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                           >  SRR4302533.3625945/1‑94 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                           <  SRR4302533.3479271/94‑1 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                          <  SRR4302533.4435657/94‑1 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                          >  SRR4302533.6314862/1‑94 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                          <  SRR4302533.4059931/94‑1 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAAAGAATTCAAGC                                          >  SRR4302533.93069/1‑94 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTCGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                          >  SRR4302533.3796688/1‑94 (MQ=60)

                                                      TGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCC                                         <  SRR4302533.5449596/94‑1 (MQ=60)

                                                       GCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCA                                        <  SRR4302533.606915/94‑1 (MQ=60)

                                                       GCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCA                                        <  SRR4302533.4277277/94‑1 (MQ=60)

                                                        CTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAA                                       >  SRR4302533.2645229/1‑94 (MQ=60)

                                                        CTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAA                                       >  SRR4302533.3387395/1‑94 (MQ=60)

                                                          ACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATG                                     <  SRR4302533.4798731/94‑1 (MQ=60)

                                                           CCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGG                                    <  SRR4302533.2322011/94‑1 (MQ=60)

                                                           CCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGG                                    <  SRR4302533.4872650/94‑1 (MQ=60)

                                                            CACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGA                                   >  SRR4302533.2429928/1‑94 (MQ=60)

                                                            CACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGA                                   >  SRR4302533.2791042/1‑94 (MQ=60)

                                                             ACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAA                                  >  SRR4302533.673676/1‑94 (MQ=60)

                                                             ACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAA                                  <  SRR4302533.728254/94‑1 (MQ=60)

                                                              CTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAGAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAA                                 >  SRR4302533.5211119/1‑94 (MQ=60)

                                                              CTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAA                                 >  SRR4302533.302954/1‑94 (MQ=60)

                                                              CTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGGGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAA                                 >  SRR4302533.4344857/1‑94 (MQ=60)

                                                                GCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGACAATGGAAATA                               >  SRR4302533.3681391/1‑94 (MQ=60)

                                                                 CGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAA                              >  SRR4302533.6184724/1‑94 (MQ=60)

                                                                 CGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAA                              <  SRR4302533.1959733/94‑1 (MQ=60)

                                                                 CGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAA                              <  SRR4302533.2293477/94‑1 (MQ=60)

                                                                 CGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAATGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAA                              >  SRR4302533.1554041/1‑94 (MQ=60)

                                                                  GAATTTAACCACTGCCTGACATGGCTCGCAATGTCGCACATTGCTTCTGGAAAGGGATGTGAGCAGGTAACGAATTCAAGCCAATGGAAATAAC                             <  SRR4302533.2805833/94‑1 (MQ=60)

                                                                   AATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGGAACGAATTCAAGCCAATGGAAATAACA                            >  SRR4302533.481390/1‑94 (MQ=60)

                                                                    ATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAA                           >  SRR4302533.5033772/1‑94 (MQ=60)

                                                                    ATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAA                           <  SRR4302533.4566194/94‑1 (MQ=60)

                                                                    ATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTACTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAA                           >  SRR4302533.3393167/1‑94 (MQ=60)

                                                                     TTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAA                          >  SRR4302533.5013432/1‑94 (MQ=60)

                                                                     TTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAA                          >  SRR4302533.4440484/1‑94 (MQ=60)

                                                                      TTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAT                         <  SRR4302533.5400495/94‑1 (MQ=60)

                                                                       TAACCACTTACTGACATGGCTCGGGAGGTCGCACATTGCTTTTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATA                        >  SRR4302533.4233134/1‑94 (MQ=60)

                                                                        AACCACTTACTGACATGGCTCGCGATGTCGGACATTGCATCTGGAAAGCAACTTCAGCAGGTAACCAATTCAAGCCAATGGAAATAACAAATAG                       <  SRR4302533.6230378/94‑1 (MQ=60)

                                                                        AACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCCGGTAACGAATTCAAGCCAATGGTAATAACAAATAG                       >  SRR4302533.1760589/1‑94 (MQ=60)

                                                                         ACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGT                      >  SRR4302533.2779599/1‑94 (MQ=60)

                                                                         ACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGT                      >  SRR4302533.5105526/1‑94 (MQ=60)

                                                                         ACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGT                      <  SRR4302533.4102347/94‑1 (MQ=60)

                                                                          CCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTT                     <  SRR4302533.856283/94‑1 (MQ=60)

                                                                           CACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTC                    <  SRR4302533.6211254/94‑1 (MQ=60)

                                                                           CACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGGTC                    >  SRR4302533.5542035/1‑94 (MQ=60)

                                                                            ACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCG                   <  SRR4302533.3051295/94‑1 (MQ=60)

                                                                            ACTTACTGACATGGCTCGCGATGTCGCACATGGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCG                   <  SRR4302533.4084834/94‑1 (MQ=60)

                                                                              TTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCT                 >  SRR4302533.5103657/1‑94 (MQ=60)

                                                                              TTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGAAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCT                 <  SRR4302533.2619284/94‑1 (MQ=60)

                                                                               TACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTC                <  SRR4302533.1363996/94‑1 (MQ=60)

                                                                                ACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCA               >  SRR4302533.362979/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT              >  SRR4302533.3983652/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT              >  SRR4302533.4091293/1‑94 (MQ=60)

                                                                                  TGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATG             <  SRR4302533.2129263/94‑1 (MQ=60)

                                                                                  TGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATG             <  SRR4302533.1586135/94‑1 (MQ=60)

                                                                                   GACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGA            >  SRR4302533.3462440/1‑94 (MQ=60)

                                                                                   GACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGA            <  SRR4302533.2171875/94‑1 (MQ=60)

                                                                                    ACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGAC           >  SRR4302533.4559436/1‑94 (MQ=60)

                                                                                     CATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACT          >  SRR4302533.2084803/1‑94 (MQ=60)

                                                                                     CATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACT          >  SRR4302533.5603764/1‑94 (MQ=60)

                                                                                      ATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTA         <  SRR4302533.5308267/94‑1 (MQ=60)

                                                                                       TGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT        <  SRR4302533.53515/94‑1 (MQ=60)

                                                                                        GGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATT       >  SRR4302533.4590135/1‑94 (MQ=60)

                                                                                        GGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATT       <  SRR4302533.3338258/94‑1 (MQ=60)

                                                                                        GGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATT       >  SRR4302533.4312352/1‑94 (MQ=60)

                                                                                        GGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGACAATGGAAATAACAAATAGTTCGCTCATGACTATT       >  SRR4302533.3383012/1‑94 (MQ=60)

                                                                                          CTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTT     >  SRR4302533.390374/1‑94 (MQ=60)

                                                                                          CTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTT     >  SRR4302533.538103/1‑94 (MQ=60)

                                                                                           TCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTT    <  SRR4302533.1203597/94‑1 (MQ=60)

                                                                                            CGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTT   <  SRR4302533.5999442/94‑1 (MQ=60)

                                                                                             GCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTTA  <  SRR4302533.721792/94‑1 (MQ=60)

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTTA  >  NC_000913/4227702‑4227888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`