Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,078	C→T	W135* (TGG→TAG)	yjjP ←	putative structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,078	0	C	T	99.8%	2190.6 / NA	557	W135* (TGG→TAG)	yjjP	putative structural protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (324/232); minor base G (0/1); total (324/233)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 559 total aligned reads displayed.

GGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT  >  NC_000913/4599987‑4600168

 gCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATc                                                                                         >  2:4741523/1‑94 (MQ=255)

 gCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATc                                                                                         <  1:7423632/94‑1 (MQ=255)

   aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                       >  1:6270270/1‑94 (MQ=255)

   aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                       >  1:3317068/1‑94 (MQ=255)

   aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                       >  1:3238197/1‑94 (MQ=255)

   aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                       >  2:6962785/1‑94 (MQ=255)

   aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGACAACCATTAAGGCTACCAGCTATCtt                                                                                       >  2:9061451/1‑94 (MQ=255)

    aagaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTg                                                                                      <  1:10460109/94‑1 (MQ=255)

      gaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggg                                                                                    >  1:5428545/1‑94 (MQ=255)

        agGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTa                                                                                  >  2:812601/1‑94 (MQ=255)

        agGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTa                                                                                  >  2:6902479/1‑94 (MQ=255)

         ggTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTaa                                                                                 <  2:3375206/94‑1 (MQ=255)

          gTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAc                                                                                <  2:8891879/94‑1 (MQ=255)

           tgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACg                                                                               >  2:3712718/1‑94 (MQ=255)

               gaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAAt                                                                           <  1:1729564/94‑1 (MQ=255)

               gaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAAt                                                                           >  1:3090947/1‑94 (MQ=255)

                aCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATg                                                                          >  1:462151/1‑94 (MQ=255)

                aCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATg                                                                          >  1:359449/1‑94 (MQ=255)

                aCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATg                                                                          <  2:9895268/94‑1 (MQ=255)

                 cGGCCCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATgg                                                                         <  1:8660602/94‑1 (MQ=255)

                 cGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATgg                                                                         <  2:2310640/94‑1 (MQ=255)

                 cGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATgg                                                                         >  2:7496468/1‑94 (MQ=255)

                 cGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATgg                                                                         >  1:7654179/1‑94 (MQ=255)

                  ggCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGc                                                                        >  1:5577230/1‑94 (MQ=255)

                   gccccATCCCAGACACCATTATTGAGTTTACAGAAACAGGCGCGAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCt                                                                       <  1:9401595/91‑1 (MQ=255)

                   gCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCt                                                                       >  1:7900961/1‑94 (MQ=255)

                    cACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTg                                                                      <  2:4973657/94‑1 (MQ=255)

                     aCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGa                                                                     >  1:1243034/1‑94 (MQ=255)

                     aCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGa                                                                     >  1:6995425/1‑94 (MQ=255)

                       cATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAt                                                                   >  2:1859447/1‑94 (MQ=255)

                        aTCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                  >  1:9724617/1‑94 (MQ=255)

                          cccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTg                                                                <  2:252727/94‑1 (MQ=255)

                            cAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGc                                                              >  1:158891/1‑94 (MQ=255)

                               ccACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaa                                                           >  2:6774975/1‑94 (MQ=255)

                                cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                          >  1:10311078/1‑94 (MQ=255)

                                cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                          <  1:1001595/94‑1 (MQ=255)

                                  ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATc                                                        <  2:7866290/94‑1 (MQ=255)

                                  ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATc                                                        >  1:6516162/1‑94 (MQ=255)

                                  ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATc                                                        <  1:3809444/94‑1 (MQ=255)

                                  ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATc                                                        >  2:8194833/1‑94 (MQ=255)

                                   cGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCg                                                       <  2:7704548/94‑1 (MQ=255)

                                     ttATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGtt                                                     >  2:6071535/1‑94 (MQ=255)

                                     ttATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGtt                                                     <  1:8503287/94‑1 (MQ=255)

                                      tATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGttt                                                    <  2:5960352/94‑1 (MQ=255)

                                      tATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGttt                                                    <  2:1138293/94‑1 (MQ=255)

                                       aTTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtc                                                   >  1:2536982/1‑94 (MQ=255)

                                          gAGTTTACAGAAACAGGCGCAAGCAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTctct                                                <  1:5839427/94‑1 (MQ=255)

                                           aGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                               >  1:5018547/1‑94 (MQ=255)

                                            gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAc                                              >  2:8314677/1‑94 (MQ=255)

                                             tttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAACGGCTGAATTTGGCTAAATCGTTTCTCTACg                                             >  1:10624581/1‑94 (MQ=255)

                                              ttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGc                                            >  1:2413717/1‑94 (MQ=255)

                                              ttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGc                                            <  1:2784637/94‑1 (MQ=255)

                                                 cAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCtt                                         >  2:5411949/1‑94 (MQ=255)

                                                     aaCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTa                                     >  2:9443067/1‑94 (MQ=255)

                                                     aaCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGCTTCTCTACGCCTTTGTa                                     >  1:1157777/1‑94 (MQ=255)

                                                      aCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTat                                    >  2:8688108/1‑93 (MQ=255)

                                                      aCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTaa                                    <  1:7232015/94‑1 (MQ=255)

                                                      aCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTaa                                    >  1:7551113/1‑94 (MQ=255)

                                                       cAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAAt                                   <  2:8569030/94‑1 (MQ=255)

                                                         ggCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGACTTAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATcc                                 <  2:10171628/94‑1 (MQ=255)

                                                          gcgcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCca                                >  1:2965492/1‑94 (MQ=255)

                                                           cgcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcag                               >  2:5179230/1‑94 (MQ=255)

                                                            gcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagc                              <  2:3169257/94‑1 (MQ=255)

                                                             cAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                             >  2:6547861/1‑94 (MQ=255)

                                                              aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                            >  1:8321286/1‑94 (MQ=255)

                                                                gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgat                          <  1:6437072/94‑1 (MQ=255)

                                                                  aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                        <  1:198730/94‑1 (MQ=255)

                                                                  aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                        >  1:1865702/1‑94 (MQ=255)

                                                                  aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                        >  1:1420206/1‑94 (MQ=255)

                                                                   agtccatccatTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatgat                       >  2:9372806/4‑94 (MQ=255)

                                                                   aGGCCAACCATTAAGGCTACCAGCTATCTTGGTTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAaatgat                       <  2:5782517/94‑1 (MQ=255)

                                                                   aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatgat                       >  2:409743/1‑94 (MQ=255)

                                                                   aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatgat                       <  1:4406366/94‑1 (MQ=255)

                                                                     gCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGc                     <  2:6389103/94‑1 (MQ=255)

                                                                      ccAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCt                    <  2:2668176/94‑1 (MQ=255)

                                                                       cAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTc                   <  1:8180614/94‑1 (MQ=255)

                                                                       cAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTc                   >  1:9233769/1‑94 (MQ=255)

                                                                       cAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATACAGCAGATGATGCTc                   >  1:3891712/1‑94 (MQ=255)

                                                                        aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGCTGCTcc                  >  2:457399/1‑94 (MQ=255)

                                                                        aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                  >  1:345223/1‑94 (MQ=255)

                                                                        aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                  <  2:4343904/94‑1 (MQ=255)

                                                                        aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                  >  1:4728081/1‑94 (MQ=255)

                                                                         aCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCg                 <  1:2168316/94‑1 (MQ=255)

                                                                          ccATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc                >  1:529469/1‑94 (MQ=255)

                                                                           cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa               <  1:4106501/94‑1 (MQ=255)

                                                                           cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa               >  1:8901419/1‑94 (MQ=255)

                                                                             ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAg             >  1:688542/1‑94 (MQ=255)

                                                                               aaGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGaa           <  2:3493127/94‑1 (MQ=255)

                                                                                aGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAt          <  1:6698937/94‑1 (MQ=255)

                                                                                 ggCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATc         <  2:10505592/94‑1 (MQ=255)

                                                                                  gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATca        <  1:731140/94‑1 (MQ=255)

                                                                                  gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATca        <  2:191273/94‑1 (MQ=255)

                                                                                   cTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATcac       <  2:1029977/94‑1 (MQ=255)

                                                                                   cTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATcac       >  1:953284/1‑94 (MQ=255)

                                                                                    tACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATcaca      >  1:5170938/1‑94 (MQ=255)

                                                                                     aCCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa     >  2:7211641/1‑94 (MQ=255)

                                                                                      ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt    >  1:9933891/1‑94 (MQ=255)

                                                                                      ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt    <  2:9643329/94‑1 (MQ=255)

                                                                                       cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg   <  2:1512192/94‑1 (MQ=255)

                                                                                        aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtgt  >  2:3026349/1‑94 (MQ=255)

GGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT  >  NC_000913/4599987‑4600168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 263 total aligned reads displayed.

GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608502‑4608684

GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAG                                                                                           <  SRR4302545.10868663/94‑1 (MQ=60)

  ATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGCCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTA‑GCG                                                                                        >  SRR4302545.8149033/1‑94 (MQ=60)

     GTCAGCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      <  SRR4302545.7214787/94‑1 (MQ=60)

     GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      <  SRR4302545.1679300/94‑1 (MQ=60)

     GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      <  SRR4302545.6815309/94‑1 (MQ=60)

      TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTG                                                                                     <  SRR4302545.8121987/94‑1 (MQ=60)

      TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTG                                                                                     <  SRR4302545.6094585/94‑1 (MQ=60)

       CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                    <  SRR4302545.10550895/94‑1 (MQ=60)

       CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                    >  SRR4302545.8942671/1‑94 (MQ=60)

        AACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCTGACCCGCGAGCATTAATTCTTGCCTCCAGGGTGCGGTAGCCGCTGCG                                                                                   >  SRR4302545.9660007/1‑94 (MQ=60)

           CTGCGCCTGGCACAGGGACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                >  SRR4302545.7468027/1‑94 (MQ=60)

           CTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                <  SRR4302545.2487359/94‑1 (MQ=60)

           CTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                >  SRR4302545.3452828/1‑94 (MQ=60)

           CTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCCCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                >  SRR4302545.4714867/1‑94 (MQ=60)

            TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               >  SRR4302545.2507170/1‑94 (MQ=60)

             GCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG                                                                              >  SRR4302545.7612348/1‑94 (MQ=60)

              CGCCTGGCACAGGAACGTTATCCGGACGTTCAGTCCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGT                                                                             <  SRR4302545.2003797/94‑1 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           <  SRR4302545.197149/94‑1 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           <  SRR4302545.6041566/94‑1 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           <  SRR4302545.5384992/94‑1 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           <  SRR4302545.4408075/94‑1 (MQ=60)

                 CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          >  SRR4302545.7946660/1‑94 (MQ=60)

                 CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          >  SRR4302545.1289669/1‑94 (MQ=60)

                 CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          >  SRR4302545.4545779/1‑94 (MQ=60)

                  TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAT                                                                         <  SRR4302545.6829653/94‑1 (MQ=60)

                  TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAT                                                                         >  SRR4302545.3971053/1‑94 (MQ=60)

                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302545.8991152/94‑1 (MQ=60)

                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302545.8671552/94‑1 (MQ=60)

                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        >  SRR4302545.491431/1‑94 (MQ=60)

                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302545.5396196/94‑1 (MQ=60)

                    GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTT                                                                       >  SRR4302545.9795781/1‑94 (MQ=60)

                     CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      <  SRR4302545.8165145/94‑1 (MQ=60)

                     CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      >  SRR4302545.8444593/1‑94 (MQ=60)

                     CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      >  SRR4302545.10029433/1‑94 (MQ=60)

                      ACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                     >  SRR4302545.2396741/1‑94 (MQ=60)

                      ACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                     <  SRR4302545.9354868/94‑1 (MQ=60)

                       CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    >  SRR4302545.880672/1‑94 (MQ=60)

                       CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    <  SRR4302545.5718737/94‑1 (MQ=60)

                       CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    >  SRR4302545.5852053/1‑94 (MQ=60)

                       CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    >  SRR4302545.5852054/1‑94 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302545.8239015/1‑94 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   <  SRR4302545.1340010/94‑1 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302545.7806266/1‑94 (MQ=60)

                         GGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTT                                                                  >  SRR4302545.1441962/1‑94 (MQ=60)

                           AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCC                                                                >  SRR4302545.7322980/1‑94 (MQ=60)

                           AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCC                                                                >  SRR4302545.3212089/1‑94 (MQ=60)

                            ACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCT                                                               <  SRR4302545.7167246/94‑1 (MQ=60)

                            ACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCT                                                               <  SRR4302545.9160147/94‑1 (MQ=60)

                             CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              >  SRR4302545.7770461/1‑94 (MQ=60)

                             CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              >  SRR4302545.5766781/1‑94 (MQ=60)

                             CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              <  SRR4302545.1753043/94‑1 (MQ=60)

                             CGTTATCCGGACGTTCAGTTCCAACAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGTGCACTGCCACTTTCCCTTCCTT                                                              >  SRR4302545.7614401/1‑94 (MQ=60)

                              GTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTA                                                             <  SRR4302545.3306659/94‑1 (MQ=60)

                              GTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGAGCCCTGTCAATTTCCCTTCCTTA                                                             >  SRR4302545.5995231/1‑94 (MQ=60)

                                TATCCGGACGTTCAGCTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           >  SRR4302545.4340018/1‑94 (MQ=60)

                                 TTCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302545.8364574/94‑1 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302545.10138894/94‑1 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302545.8364573/94‑1 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302545.3132496/1‑94 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302545.1019779/94‑1 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302545.2929241/1‑94 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302545.7539038/1‑94 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302545.2518301/1‑94 (MQ=60)

                                  TCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                         <  SRR4302545.141266/94‑1 (MQ=60)

                                  TCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                         <  SRR4302545.1384362/94‑1 (MQ=60)

                                    CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       <  SRR4302545.1447976/94‑1 (MQ=60)

                                      GACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGC                                                     >  SRR4302545.6313061/1‑94 (MQ=60)

                                       ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    <  SRR4302545.5145477/94‑1 (MQ=60)

                                       ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    <  SRR4302545.6427662/94‑1 (MQ=60)

                                       ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    <  SRR4302545.2136566/94‑1 (MQ=60)

                                        CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   <  SRR4302545.2755296/94‑1 (MQ=60)

                                        CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   <  SRR4302545.5082716/94‑1 (MQ=60)

                                        CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   >  SRR4302545.1602817/1‑94 (MQ=60)

                                         GTTCAGTTCCACTAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAGTTTCCCTTCCTTATTAGCCGCTTA                                                  <  SRR4302545.186350/94‑1 (MQ=60)

                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302545.7060024/1‑94 (MQ=60)

                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302545.10893334/94‑1 (MQ=60)

                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302545.10164706/94‑1 (MQ=60)

                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302545.647675/94‑1 (MQ=60)

                                           TCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACG                                                <  SRR4302545.6631881/94‑1 (MQ=60)

                                           TCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACG                                                <  SRR4302545.1027684/94‑1 (MQ=60)

                                             AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCACTTTGCCTTCCTTATTAGCCGCTTACGGA                                              >  SRR4302545.10192488/1‑94 (MQ=60)

                                             AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                              >  SRR4302545.771035/1‑94 (MQ=60)

                                             AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                              >  SRR4302545.10192490/1‑94 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGTGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302545.10553357/94‑1 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302545.2686747/94‑1 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302545.177938/94‑1 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302545.7338305/94‑1 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302545.4530954/94‑1 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302545.3876822/94‑1 (MQ=60)

                                                TCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATG                                           <  SRR4302545.7900714/94‑1 (MQ=60)

                                                TCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATG                                           <  SRR4302545.1905211/94‑1 (MQ=60)

                                                TCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATG                                           >  SRR4302545.6320465/1‑94 (MQ=60)

                                                 CCACTAGACCCTCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          >  SRR4302545.9617840/1‑94 (MQ=60)

                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          <  SRR4302545.9281963/94‑1 (MQ=60)

                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          <  SRR4302545.3145293/94‑1 (MQ=60)

                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          <  SRR4302545.6248112/94‑1 (MQ=60)

                                                  CACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACtgcatgat                                         >  SRR4302545.3027463/1‑86 (MQ=60)

                                                   ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTC                                        >  SRR4302545.4282154/1‑94 (MQ=60)

                                                   ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTC                                        <  SRR4302545.9345865/94‑1 (MQ=60)

                                                   ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTC                                        >  SRR4302545.1437624/1‑94 (MQ=60)

                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302545.3862719/94‑1 (MQ=60)

                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302545.4449124/94‑1 (MQ=60)

                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCA                                       <  SRR4302545.1850568/94‑1 (MQ=60)

                                                     CAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTT                                      >  SRR4302545.2760093/1‑94 (MQ=60)

                                                      AGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     <  SRR4302545.4579535/94‑1 (MQ=60)

                                                       GACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAA                                    >  SRR4302545.2383088/1‑94 (MQ=60)

                                                        GCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   <  SRR4302545.4838348/94‑1 (MQ=60)

                                                        ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   <  SRR4302545.10217955/94‑1 (MQ=60)

                                                        ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   >  SRR4302545.9444053/1‑94 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  >  SRR4302545.4603672/1‑94 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  >  SRR4302545.10801992/1‑94 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  <  SRR4302545.190420/94‑1 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 <  SRR4302545.2518833/94‑1 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302545.6164250/1‑94 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302545.8742112/1‑94 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302545.874658/1‑94 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302545.8802722/1‑94 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302545.7150437/1‑94 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302545.3976819/1‑94 (MQ=60)

                                                           CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTGCGGAATGTTCTTAAAACA                                <  SRR4302545.8719376/94‑1 (MQ=60)

                                                           CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACA                                >  SRR4302545.3246534/1‑94 (MQ=60)

                                                           CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACA                                >  SRR4302545.9596830/1‑94 (MQ=60)

                                                            GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               >  SRR4302545.5286357/1‑94 (MQ=60)

                                                            GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               >  SRR4302545.6973889/1‑94 (MQ=60)

                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              >  SRR4302545.10416583/1‑94 (MQ=60)

                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              <  SRR4302545.420330/94‑1 (MQ=60)

                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              >  SRR4302545.4615964/1‑94 (MQ=60)

                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              <  SRR4302545.5161124/94‑1 (MQ=60)

                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              <  SRR4302545.10883711/94‑1 (MQ=60)

                                                              GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             >  SRR4302545.6144552/1‑94 (MQ=60)

                                                              GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTGCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             <  SRR4302545.10824689/94‑1 (MQ=60)

                                                               AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302545.9493271/94‑1 (MQ=60)

                                                                GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTTCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           >  SRR4302545.1737908/1‑94 (MQ=60)

                                                                GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           >  SRR4302545.4328292/1‑94 (MQ=60)

                                                                GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           >  SRR4302545.1934985/1‑94 (MQ=60)

                                                                GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           >  SRR4302545.10664810/1‑94 (MQ=60)

                                                                 CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          >  SRR4302545.2490180/1‑94 (MQ=60)

                                                                 CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          <  SRR4302545.5066294/94‑1 (MQ=60)

                                                                 CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          <  SRR4302545.4858404/94‑1 (MQ=60)

                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302545.6886250/94‑1 (MQ=60)

                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302545.5384009/94‑1 (MQ=60)

                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302545.2621206/94‑1 (MQ=60)

                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        <  SRR4302545.8665245/94‑1 (MQ=60)

                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        <  SRR4302545.2373914/94‑1 (MQ=60)

                                                                       TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    >  SRR4302545.4264952/1‑94 (MQ=60)

                                                                       TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    <  SRR4302545.8819631/94‑1 (MQ=60)

                                                                       TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    <  SRR4302545.7096776/94‑1 (MQ=60)

                                                                       TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    >  SRR4302545.9401728/1‑94 (MQ=60)

                                                                        TCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                   <  SRR4302545.9836794/94‑1 (MQ=60)

                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  >  SRR4302545.7530478/1‑94 (MQ=60)

                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  <  SRR4302545.1157683/94‑1 (MQ=60)

                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  <  SRR4302545.10019807/94‑1 (MQ=60)

                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  >  SRR4302545.3428646/1‑94 (MQ=60)

                                                                          TTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTAT                 <  SRR4302545.2489984/94‑1 (MQ=60)

                                                                          TTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTAT                 <  SRR4302545.5253280/94‑1 (MQ=60)

                                                                           TGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATG                <  SRR4302545.8078416/94‑1 (MQ=60)

                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               <  SRR4302545.3081080/94‑1 (MQ=60)

                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               >  SRR4302545.3008561/1‑94 (MQ=60)

                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               >  SRR4302545.4831938/1‑94 (MQ=60)

                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               <  SRR4302545.1700830/94‑1 (MQ=60)

                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               >  SRR4302545.5165818/1‑94 (MQ=60)

                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               >  SRR4302545.9728815/1‑94 (MQ=60)

                                                                             CCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTCAAACATTCACTTTTGCTTATGCT              >  SRR4302545.7298437/1‑94 (MQ=60)

                                                                             CCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTT              <  SRR4302545.2003999/94‑1 (MQ=60)

                                                                             CCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTT              <  SRR4302545.8135372/94‑1 (MQ=60)

                                                                              CTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTT             >  SRR4302545.5957057/1‑94 (MQ=60)

                                                                               TCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT            >  SRR4302545.3495073/1‑94 (MQ=60)

                                                                               TCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT            >  SRR4302545.10419233/1‑94 (MQ=60)

                                                                               TCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT            >  SRR4302545.3212074/1‑94 (MQ=60)

                                                                               CCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT            <  SRR4302545.8147465/94‑1 (MQ=60)

                                                                                CGAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302545.1164676/94‑1 (MQ=60)

                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302545.5586546/94‑1 (MQ=60)

                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302545.9822725/94‑1 (MQ=60)

                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302545.9822726/94‑1 (MQ=60)

                                                                                 CAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG          <  SRR4302545.436195/94‑1 (MQ=60)

                                                                                 CAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG          <  SRR4302545.4552977/94‑1 (MQ=60)

                                                                                 CAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG          >  SRR4302545.7859028/1‑94 (MQ=60)

                                                                                 CAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG          <  SRR4302545.7487979/94‑1 (MQ=60)

                                                                                 CAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG          <  SRR4302545.1759208/94‑1 (MQ=60)

                                                                                  AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         <  SRR4302545.226991/94‑1 (MQ=60)

                                                                                  AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302545.8642575/1‑94 (MQ=60)

                                                                                  AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302545.3867014/1‑94 (MQ=60)

                                                                                  AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302545.4928651/1‑94 (MQ=60)

                                                                                  AGGGCACGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302545.6987880/1‑94 (MQ=60)

                                                                                   GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCT        >  SRR4302545.5818053/1‑94 (MQ=60)

                                                                                    GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTG       <  SRR4302545.2128415/94‑1 (MQ=60)

                                                                                     GCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGA      <  SRR4302545.1638906/94‑1 (MQ=60)

                                                                                     GCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGA      <  SRR4302545.5338430/94‑1 (MQ=60)

                                                                                     GCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGA      <  SRR4302545.10864890/94‑1 (MQ=60)

                                                                                      CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT     >  SRR4302545.4929763/1‑94 (MQ=60)

                                                                                      CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT     <  SRR4302545.8930429/94‑1 (MQ=60)

                                                                                      CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT     >  SRR4302545.10392495/1‑94 (MQ=60)

                                                                                       GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA    <  SRR4302545.10213557/94‑1 (MQ=60)

                                                                                       GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA    >  SRR4302545.5053747/1‑94 (MQ=60)

                                                                                       GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA    >  SRR4302545.4016441/1‑94 (MQ=60)

                                                                                       GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTACGTTTTCGCTGATA    >  SRR4302545.4537855/1‑94 (MQ=60)

                                                                                        CGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATAT   >  SRR4302545.5056160/1‑94 (MQ=60)

                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGCATGTTCTTAAAAGATTCACTTTTGCTTATGCGTTCGCTGATATC  >  SRR4302545.10823212/1‑94 (MQ=60)

                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  <  SRR4302545.9738116/94‑1 (MQ=60)

                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  <  SRR4302545.6179161/94‑1 (MQ=60)

GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608502‑4608684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`