Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,608,595 2 bp→GC intergenic (+23/‑369) prfC → / → osmY peptide chain release factor RF‑3/OsmY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,5950CG99.8% 1850.0 / ‑5.8 486intergenic (+23/‑370)prfC/osmYpeptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (225/260);  minor base T (1/0);  total (226/260)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
*NC_0009134,608,5960GC99.6% 1898.4 / NA 486intergenic (+24/‑369)prfC/osmYpeptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (225/259);  minor base A (1/1);  total (226/260)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 505 total aligned reads displayed.

TGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCC  >  NC_000913/4608505‑4608685
                                                                                          ||                                                                                         
tGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCt                                                                                       <  1:7866948/96‑1 (MQ=255)
 ggTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTg                                                                                      >  1:5520905/1‑96 (MQ=255)
  gTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                     <  2:7566032/96‑1 (MQ=255)
  gTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                     <  1:971362/96‑1 (MQ=255)
   acAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTGCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgcg                                                                                    <  2:9016637/95‑1 (MQ=255)
      aCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                                 >  2:3736425/1‑96 (MQ=255)
      aCCTGCGCCGGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                                 >  1:14249082/1‑96 (MQ=255)
        cTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTg                                                                               <  2:12473322/96‑1 (MQ=255)
         tGCGCCGGGCCCAGGAACCTTATCCGGACGTTCAGTTACACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGt                                                                              <  1:4736509/96‑1 (MQ=255)
           cgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTGGCCTCCAGGGCGCGGTAGCCGCTGCGCCCCGTCa                                                                            >  2:3257017/1‑96 (MQ=255)
            gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCaa                                                                           >  2:246865/1‑96 (MQ=255)
            gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCaa                                                                           >  1:725228/1‑96 (MQ=255)
            gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCaa                                                                           <  1:13120896/96‑1 (MQ=255)
              cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAtt                                                                         >  1:8777540/1‑96 (MQ=255)
               tGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAttt                                                                        >  1:14132265/1‑96 (MQ=255)
               tGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAttt                                                                        >  1:3539794/1‑96 (MQ=255)
                gtcacaGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTc                                                                       <  1:12875274/94‑1 (MQ=255)
                 gCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTcc                                                                      <  1:10769562/96‑1 (MQ=255)
                  cacaGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGTGGTAGCGGCGGCGACCGGTCAATTTccc                                                                     >  1:7505794/1‑96 (MQ=255)
                  cacaGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTccc                                                                     >  2:1593717/1‑96 (MQ=255)
                   acaGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                    >  2:11449069/1‑96 (MQ=255)
                     aGGAACTTTAACCGGCCGTTCAGTTCCACCAGACCCGCGAAAATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcctgc                                                                  <  1:9385685/96‑3 (MQ=255)
                     aGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttc                                                                  <  2:10943185/96‑1 (MQ=255)
                      ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcc                                                                 >  2:8375705/1‑96 (MQ=255)
                            ttATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                           <  1:8471359/96‑1 (MQ=255)
                                ccGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCg                                                       <  2:11960781/96‑1 (MQ=255)
                                 cGGCCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGc                                                      <  1:3256704/96‑1 (MQ=255)
                                  ggACGTTCAGTTCCCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCt                                                     <  2:1292786/96‑1 (MQ=255)
                                  ggACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCt                                                     >  2:8231442/1‑96 (MQ=255)
                                  ggACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCt                                                     >  1:4230133/1‑96 (MQ=255)
                                   gACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCtt                                                    >  1:9235821/1‑96 (MQ=255)
                                   gACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCtt                                                    <  2:13766440/96‑1 (MQ=255)
                                    aCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                   <  2:5086060/96‑1 (MQ=255)
                                     cGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAc                                                  >  2:5524453/1‑96 (MQ=255)
                                      gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                                 >  1:5123730/1‑96 (MQ=255)
                                      gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                                 <  2:2216902/96‑1 (MQ=255)
                                      gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                                 <  1:1595027/96‑1 (MQ=255)
                                       ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                                >  2:4336537/1‑96 (MQ=255)
                                        tCAGTTCCACCAGACCCGCGAGCATTTATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGa                                               >  1:4535854/1‑96 (MQ=255)
                                        tCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGa                                               >  2:12209120/1‑96 (MQ=255)
                                         cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                              >  2:2559652/1‑96 (MQ=255)
                                          aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                             >  1:6125272/1‑96 (MQ=255)
                                          aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                             <  2:578732/96‑1 (MQ=255)
                                            ttCCACCAGACCCGCGAGCATTATTTCGTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCAGTCAATTGCCCTTCCTCATTAGCCGCGTACGGAATGt                                           <  1:10106091/96‑1 (MQ=255)
                                            ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                           <  2:14259960/96‑1 (MQ=255)
                                            ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                           <  1:12014423/96‑1 (MQ=255)
                                             tCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGtt                                          >  1:8138406/1‑96 (MQ=255)
                                              ccaccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                         >  2:10286252/1‑96 (MQ=255)
                                               caccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCt                                        >  2:8498244/1‑96 (MQ=255)
                                                accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCtt                                       <  2:6670143/96‑1 (MQ=255)
                                                accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCtt                                       >  2:5304741/1‑96 (MQ=255)
                                                accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCtt                                       <  2:8777540/96‑1 (MQ=255)
                                                accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCtt                                       <  2:14372375/96‑1 (MQ=255)
                                                accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCtt                                       >  2:13874098/1‑96 (MQ=255)
                                                   cgccccGCGAGCCTTCATTCTTGCCTCCAGGGCGCGGCAGCCGCTGCCCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACCGCATGTTCTTaaa                                    <  1:2115809/93‑1 (MQ=255)
                                                   aGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaa                                    <  1:267737/96‑1 (MQ=255)
                                                    gACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCTCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                                   <  2:4726445/96‑1 (MQ=255)
                                                    gACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                                   >  1:11490309/1‑96 (MQ=255)
                                                     aCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAc                                  <  1:9860290/96‑1 (MQ=255)
                                                       ccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAt                                >  1:2996629/1‑96 (MQ=255)
                                                        cgcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAtt                               <  2:10760634/96‑1 (MQ=255)
                                                        cgcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAtt                               <  1:2312170/96‑1 (MQ=255)
                                                        cgcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAtt                               <  2:9554639/96‑1 (MQ=255)
                                                         gcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                              <  1:13236246/96‑1 (MQ=255)
                                                         gcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                              >  1:6883304/1‑96 (MQ=255)
                                                          cgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCa                             >  2:13561101/1‑96 (MQ=255)
                                                          cgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCa                             <  2:10607459/96‑1 (MQ=255)
                                                           gAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                            <  2:1950495/96‑1 (MQ=255)
                                                           gAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                            >  2:7366447/1‑96 (MQ=255)
                                                            aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                           <  1:5838651/96‑1 (MQ=255)
                                                              cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                         >  1:4360702/1‑96 (MQ=255)
                                                              cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                         >  1:13369214/1‑96 (MQ=255)
                                                              cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                         <  1:6584798/96‑1 (MQ=255)
                                                               cttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtttt                        >  1:11297554/2‑96 (MQ=255)
                                                                 tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                      <  1:5302715/96‑1 (MQ=255)
                                                                 tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                      <  2:2838534/96‑1 (MQ=255)
                                                                  aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCt                     >  1:5689501/1‑96 (MQ=255)
                                                                  aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCt                     >  2:9317598/1‑96 (MQ=255)
                                                                   aTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCtt                    <  1:12266761/96‑1 (MQ=255)
                                                                     tCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTAt                  <  2:6615004/96‑1 (MQ=255)
                                                                        tGCCTCCCGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtt               <  2:2062985/96‑1 (MQ=255)
                                                                          ccTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtttt             <  2:6095215/96‑1 (MQ=255)
                                                                           cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGTATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc            >  1:1768537/1‑96 (MQ=255)
                                                                           cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc            >  2:7112917/1‑96 (MQ=255)
                                                                           cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc            <  2:13111291/96‑1 (MQ=255)
                                                                           cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc            >  2:11713268/1‑96 (MQ=255)
                                                                           cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc            <  1:9014570/96‑1 (MQ=255)
                                                                            cccAGGGCGCGGTCGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCg           <  1:4923222/95‑1 (MQ=255)
                                                                              cggggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt         >  1:11000532/3‑96 (MQ=255)
                                                                              cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt         >  1:11824674/1‑96 (MQ=255)
                                                                               aGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTg        >  1:13669165/1‑96 (MQ=255)
                                                                               aGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTg        >  2:6386056/1‑96 (MQ=255)
                                                                                 ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTgtt      <  1:10286252/96‑3 (MQ=255)
                                                                                 ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGat      <  2:12300510/96‑1 (MQ=255)
                                                                                 ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGat      <  1:3459597/96‑1 (MQ=255)
                                                                                 ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGat      <  1:10539509/96‑1 (MQ=255)
                                                                                   cgcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGatat    <  1:9450079/96‑1 (MQ=255)
                                                                                    gcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATc   >  2:3484321/1‑96 (MQ=255)
                                                                                    gcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATc   <  1:13506381/96‑1 (MQ=255)
                                                                                     cgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATcc  <  1:3945554/96‑1 (MQ=255)
                                                                                          ||                                                                                         
TGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCC  >  NC_000913/4608505‑4608685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 301 total aligned reads displayed.
GCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTT  >  NC_000913/4608495‑4608695
                                                                                                    |                                                                                                    
GCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAG                                                                                                      <  SRR4302518.2295302/101‑1 (MQ=60)
GCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGNNN                                                                                                      >  SRR4302518.13058932/1‑101 (MQ=60)
GCTACCAGCATGGACAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGNNN                                                                                                      >  SRR4302518.14038372/1‑101 (MQ=60)
 CTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTNNN                                                                                                     >  SRR4302518.9879115/1‑101 (MQ=60)
 CAACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTNNN                                                                                                     >  SRR4302518.12287769/1‑101 (MQ=60)
  TGCCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCTCGAGCATTAATTCTTGCCTCCAGGGCGCGGTANNN                                                                                                    >  SRR4302518.10537115/1‑101 (MQ=60)
     NNNCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                                 <  SRR4302518.8262274/101‑1 (MQ=60)
     CAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                                 >  SRR4302518.5363024/1‑101 (MQ=60)
     CAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                                 >  SRR4302518.1629995/1‑101 (MQ=60)
      AGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTG                                                                                                >  SRR4302518.2039462/1‑101 (MQ=60)
      AGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTG                                                                                                <  SRR4302518.6178022/101‑1 (MQ=60)
      AGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTG                                                                                                >  SRR4302518.1450763/1‑101 (MQ=60)
       NNNTGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                               <  SRR4302518.11168375/101‑1 (MQ=60)
       NNNTGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCCCCAGGGCGCGGTAGCCGCTGC                                                                                               <  SRR4302518.13446477/101‑1 (MQ=60)
       NNNTGGTCAACCTGCGCCTGCCACAGGAACGTTCTCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                               <  SRR4302518.10181637/101‑1 (MQ=60)
       GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                               <  SRR4302518.1009000/101‑1 (MQ=60)
        NNNGGTCATCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCG                                                                                              <  SRR4302518.9586433/101‑1 (MQ=60)
         ATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGC                                                                                             >  SRR4302518.6365143/1‑101 (MQ=60)
         ATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGC                                                                                             >  SRR4302518.4262762/1‑101 (MQ=60)
          TGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCC                                                                                            <  SRR4302518.5178797/101‑1 (MQ=60)
           GGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGNNN                                                                                           >  SRR4302518.14405427/1‑101 (MQ=60)
           GGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                           >  SRR4302518.5801575/1‑101 (MQ=60)
            GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                                          <  SRR4302518.4590549/101‑1 (MQ=60)
            GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGCCGCGGTAGCCGCTGCGCNNN                                                                                          >  SRR4302518.13855870/1‑101 (MQ=60)
              CAACCTGCGCCGGGCCCAGGAACCTTATCCGGACGTTCAGTTACACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGT                                                                                        <  SRR4302518.4984982/101‑1 (MQ=60)
               AACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTC                                                                                       >  SRR4302518.6951761/1‑101 (MQ=60)
                NNNTGCGCCTCGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCT                                                                                      <  SRR4302518.11981615/101‑1 (MQ=60)
                ACCTGCGCCTGTCACAGGAACGCTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACCCCCTGCGCCCGGTCA                                                                                      >  SRR4302518.4626261/1‑101 (MQ=60)
                ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                                      <  SRR4302518.4546342/101‑1 (MQ=60)
                ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                                      >  SRR4302518.1224220/1‑101 (MQ=60)
                ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                                      >  SRR4302518.1671662/1‑101 (MQ=60)
                ACCTGCGCCGGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGNNN                                                                                      >  SRR4302518.14905897/1‑101 (MQ=60)
                 CGTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTNNN                                                                                     >  SRR4302518.8456673/1‑101 (MQ=60)
                  CTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAT                                                                                    >  SRR4302518.3635237/1‑101 (MQ=60)
                   TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                                   <  SRR4302518.2337425/101‑1 (MQ=60)
                   TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                                   <  SRR4302518.3336181/101‑1 (MQ=60)
                   NNNGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                                   <  SRR4302518.12854154/101‑1 (MQ=60)
                     CGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATNNN                                                                                 >  SRR4302518.13696037/1‑101 (MQ=60)
                      NNNTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                                <  SRR4302518.8376315/101‑1 (MQ=60)
                      GCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                                >  SRR4302518.753629/1‑101 (MQ=60)
                      GCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                                >  SRR4302518.5750610/1‑101 (MQ=60)
                        CTGGCACAGGAACGTTATCCGGCCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                              <  SRR4302518.2889022/101‑1 (MQ=60)
                        CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGTCGCGGTAGCCGCTGCGCCCTGTCAATTTCNNN                                                                              >  SRR4302518.9203764/1‑101 (MQ=60)
                         TGGCACATGAACGTTATCCGTACGTTCAGTTCCACCAGACCCGCGAGCGTTAATTCTTGCCTCCAGGGGGCGGTAGCCGCTGCGCCTGGTCATTTTCCCTT                                                                             >  SRR4302518.2108514/1‑101 (MQ=60)
                         TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCNNN                                                                             >  SRR4302518.12094070/1‑101 (MQ=60)
                         TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCNNN                                                                             >  SRR4302518.9880411/1‑101 (MQ=60)
                         TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCGTT                                                                             >  SRR4302518.2421606/1‑101 (MQ=60)
                         TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTT                                                                             >  SRR4302518.3705284/1‑101 (MQ=60)
                         NNNCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTT                                                                             <  SRR4302518.10409855/101‑1 (MQ=60)
                         NNNCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATGCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTT                                                                             <  SRR4302518.8658824/101‑1 (MQ=60)
                          NNNACAGGAACTTTAACCGGCCGTTCAGTTCCACCAGACCCGCGAAAATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTGC                                                                            <  SRR4302518.9839542/101‑1 (MQ=60)
                          GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGAGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCNNN                                                                            >  SRR4302518.10045255/1‑101 (MQ=60)
                           GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCC                                                                           <  SRR4302518.1368178/101‑1 (MQ=60)
                           GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTNNN                                                                           >  SRR4302518.8881054/1‑101 (MQ=60)
                           GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTNNN                                                                           >  SRR4302518.13796009/1‑101 (MQ=60)
                            CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCT                                                                          >  SRR4302518.4262053/1‑101 (MQ=60)
                             ACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                                         <  SRR4302518.3976953/101‑1 (MQ=60)
                             ACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                                         <  SRR4302518.474101/101‑1 (MQ=60)
                              nnnccccgttctcaggccaatccGTTCCACCAGACCCGCCAACATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCTCCCTGTCAATTTCCCcccctcc                                                                        <  SRR4302518.11875647/78‑8 (MQ=60)
                              CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCNNN                                                                        >  SRR4302518.10666031/1‑101 (MQ=60)
                               nnnttccgatctCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                                       <  SRR4302518.7820444/89‑1 (MQ=60)
                               AGGAACGTTATCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                                       <  SRR4302518.3408669/101‑1 (MQ=60)
                               AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                                       >  SRR4302518.1514617/1‑101 (MQ=60)
                                 GAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTANNN                                                                     >  SRR4302518.13991918/1‑101 (MQ=60)
                                 GAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTANNN                                                                     >  SRR4302518.14645997/1‑101 (MQ=60)
                                  AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGGGCGGTAGCCGCTCCGCCCTGTCAATTCCCCTTCCTTATNNN                                                                    >  SRR4302518.13798645/1‑101 (MQ=60)
                                  AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                                    >  SRR4302518.6120515/1‑101 (MQ=60)
                                  AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                                    >  SRR4302518.5693166/1‑101 (MQ=60)
                                    NNNTATCCGGACGTTCAGTTCCCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                                  <  SRR4302518.12485727/101‑1 (MQ=60)
                                    NNNTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGTC                                                                  <  SRR4302518.11621392/101‑1 (MQ=60)
                                    CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                                  <  SRR4302518.6530747/101‑1 (MQ=60)
                                    CGTTATCCGGACGGTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTANNN                                                                  >  SRR4302518.14065464/1‑101 (MQ=60)
                                     GTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGNNN                                                                 >  SRR4302518.11366881/1‑101 (MQ=60)
                                      TTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGC                                                                >  SRR4302518.5198976/1‑101 (MQ=60)
                                       nnnccgtcCGTTCAGTTCCCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGAT                                                               <  SRR4302518.12061564/93‑1 (MQ=60)
                                       TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                               <  SRR4302518.7082688/101‑1 (MQ=60)
                                       NNNCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                               <  SRR4302518.11372574/101‑1 (MQ=60)
                                        NNNCGGACGTTCAGTTCCACCAGACCCGAGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCAT                                                              <  SRR4302518.10762986/101‑1 (MQ=60)
                                        ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                              >  SRR4302518.1478852/1‑101 (MQ=60)
                                        AACCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCggnnn                                                              >  SRR4302518.12644382/1‑96 (MQ=60)
                                          CCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                            <  SRR4302518.592282/101‑1 (MQ=60)
                                          CCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                            <  SRR4302518.1878484/101‑1 (MQ=60)
                                          CCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                            <  SRR4302518.6293479/101‑1 (MQ=60)
                                          CCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTNNN                                                            >  SRR4302518.10150280/1‑101 (MQ=60)
                                           CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTNNN                                                           >  SRR4302518.8138104/1‑101 (MQ=60)
                                           CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACG                                                           <  SRR4302518.5463581/101‑1 (MQ=60)
                                            GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTANNN                                                          >  SRR4302518.7438300/1‑101 (MQ=60)
                                            GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGG                                                          >  SRR4302518.1239430/1‑101 (MQ=60)
                                            GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGG                                                          >  SRR4302518.4437458/1‑101 (MQ=60)
                                            GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGG                                                          <  SRR4302518.5178027/101‑1 (MQ=60)
                                             NNNGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                                         <  SRR4302518.12577835/101‑1 (MQ=60)
                                             GAGGTTCGGTTCGACCAGACCCGCGAGCATGAATTCTTGCCTCCAGGGCGCGGTAGCCTCTGCGCCCCGTCAATTGCCCTTCCatactaacagcttactga                                                         >  SRR4302518.2912501/1‑83 (MQ=38)
                                             GACGTTCAGTTCCCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                                         <  SRR4302518.6738969/101‑1 (MQ=60)
                                             GACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACNNN                                                         >  SRR4302518.9681480/1‑101 (MQ=60)
                                               TGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGNNN                                                       >  SRR4302518.11974989/1‑101 (MQ=60)
                                               NNNTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAACTTCCCTTCCTTATTAGCCGCTTACGGAAT                                                       <  SRR4302518.9813996/101‑1 (MQ=60)
                                               NNNTCAGTTACACCAGACCCGCGCGCCTTAACTCTTGCCTCCAGGGCGCGGTCGCCGCTGCGCCCTGTCAATTACCCTTCCTTACTCGCCGCTTACGGCAT                                                       <  SRR4302518.12098656/101‑1 (MQ=41)
                                               CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCTCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                                       <  SRR4302518.5360588/101‑1 (MQ=60)
                                               CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                                       <  SRR4302518.6495636/101‑1 (MQ=60)
                                               CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                                       <  SRR4302518.581707/101‑1 (MQ=60)
                                                GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATG                                                      >  SRR4302518.5537076/1‑101 (MQ=60)
                                                 TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                                     >  SRR4302518.6146062/1‑101 (MQ=60)
                                                 NNNAGTTCCACCAGACCCGCGAGCATTATTTCGTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCAGTCAATTGCCCTTCCTCATTAGCCGCGTACGGAATGT                                                     <  SRR4302518.10584276/101‑1 (MQ=60)
                                                 NNNAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                                     <  SRR4302518.12586822/101‑1 (MQ=60)
                                                  TTAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATNNN                                                    >  SRR4302518.11604576/1‑101 (MQ=60)
                                                  TTAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATNNN                                                    >  SRR4302518.11243603/1‑101 (MQ=60)
                                                  TCAGTTCCACCAGACCCGCGAGCATTTATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                                    >  SRR4302518.4773867/1‑101 (MQ=60)
                                                  TCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                                    <  SRR4302518.727629/101‑1 (MQ=60)
                                                  TCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                                    >  SRR4302518.2228970/1‑101 (MQ=60)
                                                  TCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                                    <  SRR4302518.976772/101‑1 (MQ=60)
                                                  NNNGTTCCCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                                    <  SRR4302518.7972930/101‑1 (MQ=60)
                                                     GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGACGCTTACGGAATGGTCTT                                                 >  SRR4302518.373288/1‑101 (MQ=60)
                                                       TCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTNNN                                               >  SRR4302518.8541991/1‑101 (MQ=60)
                                                        CCACCCGCCCCGCGAGCCTTCATTCTTGCCTCCAGGGCGCGGCAGCCGCTGCCCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACCGCATGTTCTTAAA                                              <  SRR4302518.2215491/101‑1 (MQ=58)
                                                        CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                              <  SRR4302518.149494/101‑1 (MQ=60)
                                                        CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                              <  SRR4302518.278580/101‑1 (MQ=60)
                                                         NNNCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                             <  SRR4302518.8562882/101‑1 (MQ=60)
                                                         CCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                             <  SRR4302518.5053253/101‑1 (MQ=60)
                                                          ccccgacccgcccGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                            <  SRR4302518.4052058/88‑1 (MQ=60)
                                                          NNNAGATCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCCCTTACGGAATGTTCTTAAAAC                                            <  SRR4302518.10883109/101‑1 (MQ=60)
                                                          NNNAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                            <  SRR4302518.10331627/101‑1 (MQ=60)
                                                          ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                            <  SRR4302518.5844512/101‑1 (MQ=60)
                                                          ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                            >  SRR4302518.4921406/1‑101 (MQ=60)
                                                          ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                            <  SRR4302518.36020/101‑1 (MQ=60)
                                                          ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTGACGGAATGTTCTTAAAAC                                            >  SRR4302518.1406591/1‑101 (MQ=60)
                                                           CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACA                                           >  SRR4302518.6961016/1‑101 (MQ=60)
                                                            CAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                                          >  SRR4302518.3280936/1‑101 (MQ=60)
                                                             cgcCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                                         <  SRR4302518.4452530/98‑1 (MQ=60)
                                                             AGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                                         <  SRR4302518.2424671/101‑1 (MQ=60)
                                                              NNNCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                                        <  SRR4302518.13846562/101‑1 (MQ=60)
                                                              GACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACANNN                                        >  SRR4302518.12029006/1‑101 (MQ=60)
                                                                nnngccAGCATTAATTCTTGCCTCCAGGGCCCGGCAGCCGCTGCGCCCTGTCCATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCCC                                      <  SRR4302518.10276916/95‑1 (MQ=60)
                                                                NNNGCGAGCATTAATTCTTGCCTCCAGGGCGAGGTAGCCGCTGCGCCCTGTCAATTTCCCTGCCTTATAAGCCGCTTACGGAATGTTCTTAAAACATTCCC                                      <  SRR4302518.7922062/101‑1 (MQ=60)
                                                                CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                                      <  SRR4302518.3441683/101‑1 (MQ=60)
                                                                 CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                                     >  SRR4302518.3137437/1‑101 (MQ=60)
                                                                  CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                                    >  SRR4302518.4397769/1‑101 (MQ=60)
                                                                   NNNAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                                   <  SRR4302518.10329547/101‑1 (MQ=60)
                                                                    CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                                  <  SRR4302518.5194543/101‑1 (MQ=60)
                                                                    CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                                  >  SRR4302518.7288100/1‑101 (MQ=60)
                                                                    CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                                  >  SRR4302518.3275711/1‑101 (MQ=60)
                                                                    CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTNNN                                  >  SRR4302518.13349075/1‑101 (MQ=60)
                                                                    CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTNNN                                  >  SRR4302518.14819806/1‑101 (MQ=60)
                                                                     GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTG                                 >  SRR4302518.4639192/1‑101 (MQ=60)
                                                                     GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTC                                 >  SRR4302518.6044256/1‑101 (MQ=60)
                                                                     GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTC                                 >  SRR4302518.3647783/1‑101 (MQ=60)
                                                                      AGCATTATTTCTTGCCTCCAGGGCGCGGTAGACGCTGCGCCCTATCAATCTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGC                                <  SRR4302518.5659417/101‑1 (MQ=60)
                                                                      AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTNNN                                >  SRR4302518.13530567/1‑101 (MQ=60)
                                                                      AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTNNN                                >  SRR4302518.14334182/1‑101 (MQ=60)
                                                                       GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                               <  SRR4302518.6924900/101‑1 (MQ=60)
                                                                        NNNTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                              <  SRR4302518.11246078/101‑1 (MQ=60)
                                                                        NNNTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                              <  SRR4302518.12847210/101‑1 (MQ=60)
                                                                        CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                              >  SRR4302518.4579351/1‑101 (MQ=60)
                                                                         NNNAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                             <  SRR4302518.14252986/101‑1 (MQ=60)
                                                                         CTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCNNN                             >  SRR4302518.11826639/1‑101 (MQ=60)
                                                                           TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATG                           <  SRR4302518.934582/101‑1 (MQ=60)
                                                                           TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATG                           <  SRR4302518.6742164/101‑1 (MQ=60)
                                                                           NNNTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATG                           <  SRR4302518.9735889/101‑1 (MQ=60)
                                                                            AATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTAaga                          >  SRR4302518.5978088/1‑98 (MQ=60)
                                                                            AATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT                          <  SRR4302518.2622643/101‑1 (MQ=60)
                                                                             ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTT                         <  SRR4302518.1732203/101‑1 (MQ=60)
                                                                             ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTACTAGCCGCTTACGGAATGTTCTTAAACCATTCACTTTTGCTTATGTT                         <  SRR4302518.4134876/101‑1 (MQ=60)
                                                                              TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGNNN                        >  SRR4302518.9762607/1‑101 (MQ=60)
                                                                              TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGNNN                        >  SRR4302518.14837195/1‑101 (MQ=60)
                                                                              NNNTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTT                        <  SRR4302518.13238899/101‑1 (MQ=60)
                                                                               NNNTGCCTACAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTACCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT                       <  SRR4302518.13912470/101‑1 (MQ=60)
                                                                                NNNGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC                      <  SRR4302518.9450335/101‑1 (MQ=60)
                                                                                 TTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG                     <  SRR4302518.5427679/101‑1 (MQ=60)
                                                                                  TGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTNNN                    >  SRR4302518.8947367/1‑101 (MQ=60)
                                                                                  TGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC                    <  SRR4302518.903569/101‑1 (MQ=60)
                                                                                   GCCTCCAGGGCTCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTACTAGCCGCTTACGGAATGTTCTTAAAACATTCCCTTTTGCTTATGTTTTCGCT                   <  SRR4302518.1915256/101‑1 (MQ=60)
                                                                                     CTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGTATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGA                 >  SRR4302518.1847712/1‑101 (MQ=60)
                                                                                     CTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTATTAAAACATTCACTTTTGCTTATGTTTTCGCNNN                 >  SRR4302518.9212124/1‑101 (MQ=60)
                                                                                      NNNAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGTT                <  SRR4302518.10771568/101‑1 (MQ=60)
                                                                                      NNNAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT                <  SRR4302518.11032878/101‑1 (MQ=60)
                                                                                       CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGNNN               >  SRR4302518.9469527/1‑101 (MQ=60)
                                                                                       CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA               <  SRR4302518.26560/101‑1 (MQ=60)
                                                                                        NNNGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATCT              <  SRR4302518.7748438/101‑1 (MQ=60)
                                                                                        NNNGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATAT              <  SRR4302518.11725349/101‑1 (MQ=60)
                                                                                        NNNGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATAT              <  SRR4302518.9907513/101‑1 (MQ=60)
                                                                                        CGGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGANNN              >  SRR4302518.11515590/1‑101 (MQ=60)
                                                                                         NNNGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC             <  SRR4302518.14126525/101‑1 (MQ=60)
                                                                                         CGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC             <  SRR4302518.1712655/101‑1 (MQ=60)
                                                                                         AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATNNN             >  SRR4302518.8911393/1‑101 (MQ=60)
                                                                                         AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATNNN             >  SRR4302518.13592643/1‑101 (MQ=60)
                                                                                         AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATNNN             >  SRR4302518.14186190/1‑101 (MQ=60)
                                                                                          GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCC            <  SRR4302518.4133855/101‑1 (MQ=60)
                                                                                          GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCC            <  SRR4302518.3408357/101‑1 (MQ=60)
                                                                                          GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTACTTATGTTTTCGCTGATANNN            >  SRR4302518.10827333/1‑101 (MQ=60)
                                                                                           GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCC           <  SRR4302518.1129465/101‑1 (MQ=60)
                                                                                           GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCC           <  SRR4302518.958812/101‑1 (MQ=60)
                                                                                            GCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTTCTTATTAGCCGCTTACGGAATGGTCTTAAACCATTCACTTTTGCTTATGTTTTCGCTGATATCCCG          <  SRR4302518.1594969/101‑1 (MQ=60)
                                                                                             CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCNNN         >  SRR4302518.12612929/1‑101 (MQ=60)
                                                                                              GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCNNN        >  SRR4302518.13974193/1‑101 (MQ=60)
                                                                                               CGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGNNN       >  SRR4302518.10609469/1‑101 (MQ=60)
                                                                                               CGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGC       <  SRR4302518.2933890/101‑1 (MQ=60)
                                                                                                GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGANNN      >  SRR4302518.14124961/1‑101 (MQ=60)
                                                                                                GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCG      >  SRR4302518.3316580/1‑101 (MQ=60)
                                                                                                GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCG      >  SRR4302518.5542509/1‑101 (MQ=60)
                                                                                                GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCG      >  SRR4302518.4944288/1‑101 (MQ=60)
                                                                                                   NNNCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGAT   <  SRR4302518.11318595/101‑1 (MQ=60)
                                                                                                    |                                                                                                    
GCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTT  >  NC_000913/4608495‑4608695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑