Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,608,595	2 bp→GC	intergenic (+23/‑369)	prfC → / → osmY	peptide chain release factor RF‑3/OsmY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,608,595	0	C	G	100.0%	776.2 / NA	226	intergenic (+23/‑370)	prfC/osmY	peptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (103/123); total (103/123)
*	NC_000913	4,608,596	0	G	C	100.0%	794.6 / NA	226	intergenic (+24/‑369)	prfC/osmY	peptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (103/123); total (103/123)

Only 100 of 237 total aligned reads displayed.

TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT  >  NC_000913/4608508‑4608681

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tCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCt                                                                                   >  1:583240/1‑93 (MQ=255)

  aaCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                 >  2:5044134/1‑93 (MQ=255)

  aaCCTGCGCCTGGAACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                 >  1:4504078/1‑93 (MQ=255)

   aCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgcg                                                                                >  2:385359/1‑93 (MQ=255)

   aCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgac                                                                                >  1:5659087/1‑91 (MQ=255)

    ccTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgcgc                                                                               <  2:736802/93‑1 (MQ=255)

     cTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGcc                                                                              >  1:3138429/1‑93 (MQ=255)

      tGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                             <  2:350150/93‑1 (MQ=255)

      tGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                             >  1:2199956/1‑93 (MQ=255)

       gcgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCt                                                                            <  1:1132398/93‑1 (MQ=255)

       gcgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCt                                                                            <  1:1042523/93‑1 (MQ=255)

       gcgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCt                                                                            <  2:4803299/93‑1 (MQ=255)

          ccTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTc                                                                         >  2:436846/1‑93 (MQ=255)

          ccTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTc                                                                         <  2:217128/93‑1 (MQ=255)

          ccTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTc                                                                         <  1:2384143/93‑1 (MQ=255)

           cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCa                                                                        >  1:2558017/1‑93 (MQ=255)

           cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCa                                                                        >  1:378347/1‑93 (MQ=255)

           cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCa                                                                        >  1:3650732/1‑93 (MQ=255)

            tGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCaa                                                                       <  1:6291652/93‑1 (MQ=255)

             ggCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAt                                                                      <  1:1268574/93‑1 (MQ=255)

              gCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAtt                                                                     <  1:5786559/93‑1 (MQ=255)

                acaGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTc                                                                   >  1:1775805/1‑93 (MQ=255)

                 caGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTcc                                                                  >  2:916811/1‑93 (MQ=255)

                  aGGGACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTccc                                                                 >  2:5252125/1‑93 (MQ=255)

                  aGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTccc                                                                 <  1:6234546/93‑1 (MQ=255)

                   ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                >  1:2067293/1‑93 (MQ=255)

                   ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                <  2:542347/93‑1 (MQ=255)

                   ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                >  1:4942581/1‑93 (MQ=255)

                      aCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcc                                                             <  1:3689755/93‑1 (MQ=255)

                       cGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcct                                                            <  1:805887/93‑1 (MQ=255)

                       cGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcct                                                            <  2:598481/93‑1 (MQ=255)

                        gTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcctt                                                           >  2:649052/1‑93 (MQ=255)

                              cGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGc                                                     <  2:4217387/93‑1 (MQ=255)

                               ggACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGcc                                                    <  2:933396/93‑1 (MQ=255)

                               ggACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGcc                                                    <  1:4395457/93‑1 (MQ=255)

                               ggACGTTCAGTGCCCCCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGcc                                                    <  2:5849726/93‑1 (MQ=255)

                                gACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCg                                                   >  2:5091173/1‑93 (MQ=255)

                                 aCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGc                                                  <  2:190370/93‑1 (MQ=255)

                                   gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCtt                                                >  2:279476/1‑93 (MQ=255)

                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCCATTTTCCCTCCTTATTAGCCGCTTa                                               >  1:2938556/1‑93 (MQ=255)

                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                               >  1:4708611/1‑93 (MQ=255)

                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                               <  1:1678935/93‑1 (MQ=255)

                                      tacttCCACCAGACCCGCGCGCATTAATCCTCGCCTCCACGGCGCGGTACCCGCTGCGCCGTGTCAATCTCCCTTCCTTATTAGCCGCTTACg                                             <  2:4836646/90‑1 (MQ=255)

                                      cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                             <  1:5155153/93‑1 (MQ=255)

                                        gTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCACTTTCCCTTCCTTATTAGCCGCTTACGGa                                           >  2:219229/1‑93 (MQ=255)

                                        gTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGa                                           >  1:1821101/1‑93 (MQ=255)

                                        gTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGa                                           >  2:3186449/1‑93 (MQ=255)

                                          tCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                         <  2:1226788/93‑1 (MQ=255)

                                          tCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                         <  2:1986487/93‑1 (MQ=255)

                                             accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGtt                                      >  2:1758343/1‑93 (MQ=255)

                                              ccaGCCCCGCGAGCATTAATTCTTGCCTCGAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                     <  2:1819963/93‑1 (MQ=255)

                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                     <  1:2603356/93‑1 (MQ=255)

                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                     >  1:5646961/1‑93 (MQ=255)

                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGGAGCCGCTGCGCCCTGTCAATTTACCTTCCTTATTAGCCGCTTACGGAATGCTc                                     >  2:601695/1‑93 (MQ=255)

                                                aGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCtt                                   <  1:3454391/93‑1 (MQ=255)

                                                   cccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaa                                <  1:2808544/93‑1 (MQ=255)

                                                   cccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaa                                <  2:5678346/93‑1 (MQ=255)

                                                    ccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                               <  1:5212404/93‑1 (MQ=255)

                                                    ccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                               <  2:4893535/93‑1 (MQ=255)

                                                     cgcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAc                              >  1:1722065/1‑93 (MQ=255)

                                                     cgcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAc                              <  2:6445313/93‑1 (MQ=255)

                                                       cgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAt                            <  1:5511566/93‑1 (MQ=255)

                                                       cgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAt                            >  2:787146/1‑93 (MQ=255)

                                                         aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                          >  1:270027/1‑93 (MQ=255)

                                                         aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                          >  1:4826753/1‑93 (MQ=255)

                                                         aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                          >  2:372104/1‑93 (MQ=255)

                                                          gCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCa                         <  2:4063149/93‑1 (MQ=255)

                                                           cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTTAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                        <  2:6381141/93‑1 (MQ=255)

                                                           cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                        >  2:2317081/1‑93 (MQ=255)

                                                           cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                        <  1:939490/93‑1 (MQ=255)

                                                            aTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                       >  1:2849739/1‑93 (MQ=255)

                                                            aTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                       <  1:3312533/93‑1 (MQ=255)

                                                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtt                      <  2:3592183/93‑1 (MQ=255)

                                                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtt                      <  2:5618918/93‑1 (MQ=255)

                                                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtt                      >  2:4087695/1‑93 (MQ=255)

                                                              tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                     <  2:112492/93‑1 (MQ=255)

                                                              tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                     >  1:2429624/1‑93 (MQ=255)

                                                              tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                     >  2:4420007/1‑93 (MQ=255)

                                                              tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                     <  2:1703137/93‑1 (MQ=255)

                                                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtttt                    >  1:1983998/1‑93 (MQ=255)

                                                                 ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                  >  1:1188526/1‑93 (MQ=255)

                                                                 ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                  <  1:318450/93‑1 (MQ=255)

                                                                  tCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCt                 <  2:4598465/93‑1 (MQ=255)

                                                                    ttGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTa               >  1:5768755/1‑93 (MQ=255)

                                                                        cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtt           >  2:229239/1‑93 (MQ=255)

                                                                         tCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGttt          >  2:5291391/1‑93 (MQ=255)

                                                                         tCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGttt          <  1:4091842/93‑1 (MQ=255)

                                                                          ccAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtttt         <  1:2465288/93‑1 (MQ=255)

                                                                           cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc        <  2:2887745/93‑1 (MQ=255)

                                                                             gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc      >  1:3991177/1‑93 (MQ=255)

                                                                             gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc      <  2:3340101/93‑1 (MQ=255)

                                                                             gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc      <  2:2422948/93‑1 (MQ=255)

                                                                             gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc      >  2:1001572/1‑93 (MQ=255)

                                                                             gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc      >  2:1550943/1‑93 (MQ=255)

                                                                              ggCGCGTTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt     <  2:3928803/93‑1 (MQ=255)

                                                                              ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt     <  2:5460064/93‑1 (MQ=255)

                                                                              ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt     <  1:3114742/93‑1 (MQ=255)

                                                                              ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt     >  1:4561809/1‑93 (MQ=255)

                                                                                 gcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGat  >  2:2162501/1‑93 (MQ=255)

                                                                                 gcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGat  >  2:3700015/1‑93 (MQ=255)

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TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT  >  NC_000913/4608508‑4608681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608502‑4608684

GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAG                                                                                           <  SRR4302537.3087979/94‑1 (MQ=60)

     GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      >  SRR4302537.3180121/1‑94 (MQ=60)

     GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      >  SRR4302537.587227/1‑94 (MQ=60)

       CAACCTGCGCCTGGAACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                    >  SRR4302537.4535225/1‑94 (MQ=60)

        AACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCG                                                                                   >  SRR4302537.2921246/1‑94 (MQ=60)

         ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGC                                                                                  >  SRR4302537.2671522/1‑94 (MQ=60)

          CCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCC                                                                                 >  SRR4302537.3160454/1‑94 (MQ=60)

           CTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                >  SRR4302537.2215462/1‑94 (MQ=60)

             GCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG                                                                              <  SRR4302537.1140260/94‑1 (MQ=60)

             GCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG                                                                              <  SRR4302537.1049721/94‑1 (MQ=60)

              CGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGT                                                                             >  SRR4302537.173089/1‑94 (MQ=60)

              CGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGT                                                                             >  SRR4302537.3195049/1‑94 (MQ=60)

               GCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTC                                                                            >  SRR4302537.3649584/1‑94 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302537.2575948/1‑94 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           <  SRR4302537.2400938/94‑1 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302537.3676432/1‑94 (MQ=60)

                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302537.380969/1‑94 (MQ=60)

                 CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          >  SRR4302537.2561294/1‑94 (MQ=60)

                 CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          <  SRR4302537.4677840/94‑1 (MQ=60)

                 CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          >  SRR4302537.6079046/1‑94 (MQ=60)

                  TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAT                                                                         <  SRR4302537.6334765/94‑1 (MQ=60)

                  TGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAT                                                                         <  SRR4302537.2857132/94‑1 (MQ=60)

                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302537.5321174/94‑1 (MQ=60)

                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302537.1277399/94‑1 (MQ=60)

                    GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTT                                                                       <  SRR4302537.5826235/94‑1 (MQ=60)

                     CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      >  SRR4302537.1788170/1‑94 (MQ=60)

                      ACAGGAACGTAATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                     >  SRR4302537.6514735/1‑94 (MQ=60)

                       CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    <  SRR4302537.5012678/94‑1 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302537.4976665/1‑94 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302537.3442814/1‑94 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   <  SRR4302537.6277427/94‑1 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   <  SRR4302537.947738/94‑1 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302537.1114007/1‑94 (MQ=60)

                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302537.2081816/1‑94 (MQ=60)

                            ACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCT                                                               <  SRR4302537.3715711/94‑1 (MQ=60)

                            ACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCT                                                               >  SRR4302537.125655/1‑94 (MQ=60)

                             CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              <  SRR4302537.811418/94‑1 (MQ=60)

                               TTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                            >  SRR4302537.369886/1‑94 (MQ=60)

                                TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           >  SRR4302537.1267438/1‑94 (MQ=60)

                                TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           >  SRR4302537.2449533/1‑94 (MQ=60)

                                TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           <  SRR4302537.5716691/94‑1 (MQ=60)

                                TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           >  SRR4302537.2386695/1‑94 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302537.5843299/1‑94 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302537.2970060/94‑1 (MQ=60)

                                 ATCCGGACGTTCAGTTCCACCAGAACGGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302537.4562283/1‑94 (MQ=60)

                                 AGCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302537.1747945/94‑1 (MQ=60)

                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      >  SRR4302537.2958701/1‑94 (MQ=60)

                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      >  SRR4302537.3144989/1‑94 (MQ=60)

                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      <  SRR4302537.4425780/94‑1 (MQ=60)

                                       ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    <  SRR4302537.5167502/94‑1 (MQ=60)

                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCCATTTTCCCTCCTTATTAGCCGCTTA                                                  >  SRR4302537.2959184/1‑94 (MQ=60)

                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302537.1792648/1‑94 (MQ=60)

                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302537.4741188/1‑94 (MQ=60)

                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302537.2496846/94‑1 (MQ=60)

                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302537.4297910/94‑1 (MQ=60)

                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302537.1690684/94‑1 (MQ=60)

                                           TCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACG                                                >  SRR4302537.214974/1‑94 (MQ=60)

                                            CAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGG                                               <  SRR4302537.5190673/94‑1 (MQ=60)

                                             AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                              >  SRR4302537.1833795/1‑94 (MQ=60)

                                              GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAA                                             >  SRR4302537.5678546/1‑94 (MQ=60)

                                              GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAA                                             >  SRR4302537.841806/1‑94 (MQ=60)

                                              GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAA                                             >  SRR4302537.2603087/1‑94 (MQ=60)

                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302537.6036091/94‑1 (MQ=60)

                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          >  SRR4302537.3925548/1‑94 (MQ=60)

                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          <  SRR4302537.449795/94‑1 (MQ=60)

                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          <  SRR4302537.5412958/94‑1 (MQ=60)

                                                  CACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                         >  SRR4302537.3656376/1‑94 (MQ=60)

                                                   ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTT                                        >  SRR4302537.4622502/1‑94 (MQ=60)

                                                   ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTC                                        >  SRR4302537.5685607/1‑94 (MQ=60)

                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302537.2621560/94‑1 (MQ=60)

                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302537.3931234/94‑1 (MQ=60)

                                                     CAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTT                                      <  SRR4302537.379374/94‑1 (MQ=60)

                                                      AGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     <  SRR4302537.3478822/94‑1 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  <  SRR4302537.2828179/94‑1 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  <  SRR4302537.4081748/94‑1 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  <  SRR4302537.277621/94‑1 (MQ=60)

                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  <  SRR4302537.3232729/94‑1 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302537.1734099/1‑94 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 <  SRR4302537.5248244/94‑1 (MQ=60)

                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 >  SRR4302537.1168347/1‑94 (MQ=60)

                                                            GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTAAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               <  SRR4302537.480601/94‑1 (MQ=60)

                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              <  SRR4302537.5549200/94‑1 (MQ=60)

                                                              GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             >  SRR4302537.4860092/1‑94 (MQ=60)

                                                              GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             >  SRR4302537.271928/1‑94 (MQ=60)

                                                               AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302537.5796104/94‑1 (MQ=60)

                                                               AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302537.2321673/94‑1 (MQ=60)

                                                               AGCATTAATTCTTCCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302537.1332045/94‑1 (MQ=60)

                                                                GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302537.4956637/94‑1 (MQ=60)

                                                                 CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          >  SRR4302537.2869694/1‑94 (MQ=60)

                                                                 CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          <  SRR4302537.945972/94‑1 (MQ=60)

                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302537.1990727/94‑1 (MQ=60)

                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         >  SRR4302537.6314423/1‑94 (MQ=60)

                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302537.3335966/94‑1 (MQ=60)

                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        >  SRR4302537.2446744/1‑94 (MQ=60)

                                                                    TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       >  SRR4302537.1997786/1‑94 (MQ=60)

                                                                    TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       <  SRR4302537.4199979/94‑1 (MQ=60)

                                                                      ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGC                     >  SRR4302537.1196781/1‑94 (MQ=60)

                                                                      ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGC                     <  SRR4302537.207314/94‑1 (MQ=60)

                                                                      ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGC                     <  SRR4302537.1740801/94‑1 (MQ=60)

                                                                       TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    <  SRR4302537.320657/94‑1 (MQ=60)

                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  >  SRR4302537.5808292/1‑94 (MQ=60)

                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  <  SRR4302537.5821454/94‑1 (MQ=60)

                                                                          TTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTAT                 <  SRR4302537.2810355/94‑1 (MQ=60)

                                                                               TCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT            <  SRR4302537.4120419/94‑1 (MQ=60)

                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           >  SRR4302537.2081782/1‑94 (MQ=60)

                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302537.2482620/94‑1 (MQ=60)

                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302537.2402813/94‑1 (MQ=60)

                                                                                  AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302537.4019124/1‑94 (MQ=60)

                                                                                   GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCT        >  SRR4302537.4593386/1‑94 (MQ=60)

                                                                                   GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCT        <  SRR4302537.5688531/94‑1 (MQ=60)

                                                                                    GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTG       <  SRR4302537.6471718/94‑1 (MQ=60)

                                                                                    GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTG       <  SRR4302537.3136496/94‑1 (MQ=60)

                                                                                    GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCGTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTG       <  SRR4302537.5247806/94‑1 (MQ=60)

                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  SRR4302537.3993514/1‑94 (MQ=60)

                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  SRR4302537.437904/1‑94 (MQ=60)

GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608502‑4608684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`