Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,141,015 T→C Q859R (CAG→CGG)  rne ← RNase E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,0150TC99.6% 918.7 / ‑5.1 268Q859R (CAG→CGG) rneRNase E
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (130/137);  total (131/137)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

Only 100 of 290 total aligned reads displayed.

ACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACG  >  NC_000913/1140925‑1141104
                                                                                          |                                                                                         
acaacTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGcgccgc                                                                                         >  1:4338485/1‑93 (MQ=255)
    cTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCtt                                                                                     >  2:6451961/1‑93 (MQ=255)
     tGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTc                                                                                    <  2:8032336/93‑1 (MQ=255)
     cggCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTc                                                                                    <  1:5688221/92‑1 (MQ=255)
      ggCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTTCTGTTCGCGCCCCTCTTCa                                                                                   >  1:3113113/1‑93 (MQ=255)
      ggCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCa                                                                                   <  1:5913388/93‑1 (MQ=255)
      ggCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCa                                                                                   >  2:6979482/1‑93 (MQ=255)
        cgcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAAc                                                                                 <  2:5226013/93‑1 (MQ=255)
        cgcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAAc                                                                                 <  1:7288796/93‑1 (MQ=255)
         gcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAAcc                                                                                >  2:6020962/1‑93 (MQ=255)
         gcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAAcc                                                                                >  2:6331892/1‑93 (MQ=255)
         gcgcTAACAACCGGTTCGAGAGCGGCGGCGACTGGGACCTCAGTCACCATCGGCTGCACATGTACTTCCTTCTGTTCGCGCCGCTCTTCAAcc                                                                                >  1:7802196/1‑93 (MQ=255)
          cgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCt                                                                               <  1:3826685/93‑1 (MQ=255)
          cgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCt                                                                               <  2:6164035/93‑1 (MQ=255)
           gcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTg                                                                              <  2:7996150/93‑1 (MQ=255)
           gcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTg                                                                              >  1:1649496/1‑93 (MQ=255)
            cTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGt                                                                             >  1:6959966/1‑93 (MQ=255)
            cTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGt                                                                             <  1:3235638/93‑1 (MQ=255)
             tAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTa                                                                            <  1:933027/93‑1 (MQ=255)
               acaacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACa                                                                          >  2:4374386/1‑93 (MQ=255)
               acaacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACa                                                                          <  2:2048419/93‑1 (MQ=255)
               acaacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACa                                                                          <  2:1961237/93‑1 (MQ=255)
                caacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACAt                                                                         <  1:5383813/93‑1 (MQ=255)
                 aacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATc                                                                        >  1:4759653/1‑93 (MQ=255)
                  acCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCt                                                                       <  1:106330/93‑1 (MQ=255)
                  acCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCt                                                                       <  2:1718301/93‑1 (MQ=255)
                  acCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCt                                                                       <  2:8106072/93‑1 (MQ=255)
                   ccGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCtt                                                                      <  1:6933975/93‑1 (MQ=255)
                     ggTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGc                                                                    >  2:2874844/1‑93 (MQ=255)
                     ggTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGc                                                                    <  1:4892051/93‑1 (MQ=255)
                      gTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCg                                                                   <  2:6226685/93‑1 (MQ=255)
                      gTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCg                                                                   >  1:2181073/1‑93 (MQ=255)
                      gTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACACGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCg                                                                   <  1:6613889/93‑1 (MQ=255)
                       ttCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCgg                                                                  <  2:7449693/93‑1 (MQ=255)
                        tCGATAGCGGCGGCTACAGGGCCCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGAGCCGCTCGTAAACCTGTACATATTGCGGa                                                                 <  2:5425801/93‑1 (MQ=255)
                        tCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGa                                                                 <  1:808673/93‑1 (MQ=255)
                        tCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGa                                                                 <  2:1061884/93‑1 (MQ=255)
                        tCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGa                                                                 <  2:14893/93‑1 (MQ=255)
                        tCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGa                                                                 <  2:8380335/93‑1 (MQ=255)
                           aTAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGt                                                              >  1:1360569/1‑93 (MQ=255)
                            tAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTa                                                             <  1:3866931/93‑1 (MQ=255)
                               cggcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACaa                                                          <  1:2941892/93‑1 (MQ=255)
                                ggcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAAt                                                         <  2:5654605/93‑1 (MQ=255)
                                ggcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGGACAAt                                                         >  2:4676593/1‑93 (MQ=255)
                                 gcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAAtt                                                        <  2:212090/93‑1 (MQ=255)
                                 gcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAAtt                                                        >  1:3403926/1‑93 (MQ=255)
                                    gcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGa                                                     <  1:3646222/93‑1 (MQ=255)
                                     cgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGAt                                                    >  1:7163802/1‑93 (MQ=255)
                                     cgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGAt                                                    <  1:6303033/93‑1 (MQ=255)
                                      gACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATa                                                   <  2:8611143/93‑1 (MQ=255)
                                      gACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATa                                                   <  1:7622386/93‑1 (MQ=255)
                                       aCAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAg                                                  >  1:3751756/1‑93 (MQ=255)
                                        cAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAga                                                 >  2:2611057/1‑92 (MQ=255)
                                        cAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGc                                                 <  2:8547668/93‑1 (MQ=255)
                                        cAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGc                                                 >  2:6864251/1‑93 (MQ=255)
                                          gggACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCgg                                               <  2:648651/93‑1 (MQ=255)
                                            gACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGAt                                             >  1:8022536/1‑93 (MQ=255)
                                            gACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGAt                                             >  2:5361966/1‑93 (MQ=255)
                                               cTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCa                                          <  1:6874288/93‑1 (MQ=255)
                                               cTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCa                                          <  2:4873406/93‑1 (MQ=255)
                                                tCAGTCACCATCGGCTGCACCTGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTCACTCTCGCGGCCGTACAATTGGATAGCGGATCCAg                                         >  1:8494989/1‑93 (MQ=255)
                                                   gTCCCCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACt                                      <  1:6798784/93‑1 (MQ=255)
                                                    tCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACtt                                     <  2:7223888/93‑1 (MQ=255)
                                                     cACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACttt                                    >  1:2436489/1‑93 (MQ=255)
                                                        cATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGcc                                 <  2:3545039/93‑1 (MQ=255)
                                                         aTCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCa                                <  1:6700240/93‑1 (MQ=255)
                                                            ggCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCagag                             >  1:1934880/1‑93 (MQ=255)
                                                             gCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAgg                            >  1:2100168/1‑93 (MQ=255)
                                                             gCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAgg                            >  1:5541233/1‑93 (MQ=255)
                                                             gCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAgg                            >  2:1308845/1‑93 (MQ=255)
                                                               tGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGGCTTTGCCAGAGGcc                          >  1:1304908/1‑93 (MQ=255)
                                                               tGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGcc                          >  2:3928484/1‑93 (MQ=255)
                                                               tGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGcc                          <  2:4493555/93‑1 (MQ=255)
                                                               tGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGcc                          >  1:5226184/1‑93 (MQ=255)
                                                                gCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGTATCCACACTTTGCCAGAGGCCa                         >  1:5008636/1‑93 (MQ=255)
                                                                gCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCa                         >  1:332099/1‑93 (MQ=255)
                                                                    aTGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTc                     <  2:3837064/93‑1 (MQ=255)
                                                                    aTGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTc                     <  2:7069971/93‑1 (MQ=255)
                                                                    aTGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTc                     >  1:8820265/1‑93 (MQ=255)
                                                                       tACTTCTCCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCgg                  <  2:2324848/93‑1 (MQ=255)
                                                                        tcttcttCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGga                 <  2:7278394/92‑1 (MQ=255)
                                                                          ttcttcCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGgaga               <  1:8506794/93‑1 (MQ=255)
                                                                          ttcttcCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAAGTCCTgaga               >  1:6407953/1‑93 (MQ=255)
                                                                           tcttcCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGTACGTACAATTGGATAGCGAATCCAGACTTTGCCAGAGGCCACTTCCTGAAAc              >  1:3774756/1‑93 (MQ=255)
                                                                            cttcCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAcg             <  2:5556872/93‑1 (MQ=255)
                                                                              tcCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAcgcg           <  2:6717859/93‑1 (MQ=255)
                                                                              tcCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAcgcg           >  2:3069430/1‑93 (MQ=255)
                                                                               ccTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACgcgc          >  1:1024789/1‑93 (MQ=255)
                                                                                cTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCa         >  1:2840292/1‑93 (MQ=255)
                                                                                 tGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCAc        <  1:5571773/93‑1 (MQ=255)
                                                                                  gTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACg       >  2:5792191/1‑93 (MQ=255)
                                                                                   tccgctCTTCTCTTCAACCTGTACATCTTGCGGACGTACAAGTGGATAGCGGTTCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGc      <  2:3209078/91‑1 (MQ=255)
                                                                                    tCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTGTGCCAGAGGCCAGTTCCGGAGACGCGCACGCt     <  1:8310966/93‑1 (MQ=255)
                                                                                     cgcgCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTa    >  2:7734401/1‑93 (MQ=255)
                                                                                     cgcgCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTa    >  2:2094347/1‑93 (MQ=255)
                                                                                     cgcgCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTa    <  1:1518237/93‑1 (MQ=255)
                                                                                      gcgcCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTAc   <  1:2572710/93‑1 (MQ=255)
                                                                                      gcgcCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTAc   >  1:4416072/1‑93 (MQ=255)
                                                                                      gcgcCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGAGGATCCAGACTTTGCCAGAGGCCAGTACCGGAGCCGAGCCCGCTAc   >  1:7484152/1‑93 (MQ=255)
                                                                                       cgccgcTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACg  <  1:4223346/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACG  >  NC_000913/1140925‑1141104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCA  >  NC_000913/1140922‑1141108
                                                                                             |                                                                                             
TCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCC                                                                                               <  SRR4302541.7459196/94‑1 (MQ=60)
 CAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCG                                                                                              >  SRR4302541.6579939/1‑94 (MQ=60)
 CAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCG                                                                                              >  SRR4302541.7024571/1‑94 (MQ=60)
  AACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGC                                                                                             >  SRR4302541.2395333/1‑94 (MQ=60)
  AACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGC                                                                                             >  SRR4302541.4381800/1‑94 (MQ=60)
   ACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCT                                                                                            <  SRR4302541.3840718/94‑1 (MQ=60)
       CTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTC                                                                                        <  SRR4302541.1327717/94‑1 (MQ=60)
       CTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTC                                                                                        >  SRR4302541.2914459/1‑94 (MQ=60)
        TGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTTCTGTTCGCGCCCCTCTTCA                                                                                       >  SRR4302541.3144630/1‑94 (MQ=60)
        TGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCA                                                                                       <  SRR4302541.1256333/94‑1 (MQ=60)
        TGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCA                                                                                       <  SRR4302541.3369293/94‑1 (MQ=60)
        CGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCA                                                                                       <  SRR4302541.5746255/94‑1 (MQ=60)
         GGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAA                                                                                      <  SRR4302541.5973589/94‑1 (MQ=60)
         GGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAA                                                                                      >  SRR4302541.3904721/1‑94 (MQ=60)
           CGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACC                                                                                    >  SRR4302541.1959431/1‑94 (MQ=60)
           CGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACC                                                                                    <  SRR4302541.7366584/94‑1 (MQ=60)
           CGCGCTAACAACCGGTTCGAGAGCGGCGGCGACTGGGACCTCAGTCACCATCGGCTGCACATGTACTTCCTTCTGTTCGCGCCGCTCTTCAACC                                                                                    >  SRR4302541.7886245/1‑94 (MQ=60)
             CGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTG                                                                                  >  SRR4302541.1665790/1‑94 (MQ=60)
             CGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTG                                                                                  <  SRR4302541.5430764/94‑1 (MQ=60)
             CGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTG                                                                                  <  SRR4302541.3865238/94‑1 (MQ=60)
              GCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGT                                                                                 >  SRR4302541.7034157/1‑94 (MQ=60)
               CTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTA                                                                                <  SRR4302541.3268311/94‑1 (MQ=60)
               CTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTA                                                                                <  SRR4302541.3091469/94‑1 (MQ=60)
               CTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTA                                                                                <  SRR4302541.8148178/94‑1 (MQ=60)
                TAACAACCGGTTCGCTAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTAC                                                                               <  SRR4302541.704546/94‑1 (MQ=60)
                TAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTAC                                                                               <  SRR4302541.941848/94‑1 (MQ=60)
                TAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTAC                                                                               >  SRR4302541.3157524/1‑94 (MQ=60)
                TAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTAC                                                                               >  SRR4302541.2795981/1‑94 (MQ=60)
                 AACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCCACCTGTACA                                                                              >  SRR4302541.921874/1‑94 (MQ=60)
                  ACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACAT                                                                             <  SRR4302541.2885455/94‑1 (MQ=60)
                  ACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACAT                                                                             >  SRR4302541.7609325/1‑94 (MQ=60)
                  ACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACAT                                                                             <  SRR4302541.7057009/94‑1 (MQ=60)
                  ACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCCCTCTTCAACCTGTACAT                                                                             >  SRR4302541.4416762/1‑94 (MQ=60)
                   CAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATC                                                                            <  SRR4302541.5439079/94‑1 (MQ=60)
                   CAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATC                                                                            >  SRR4302541.4808265/1‑94 (MQ=60)
                    AACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCT                                                                           >  SRR4302541.8903115/1‑94 (MQ=60)
                    AACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCT                                                                           >  SRR4302541.7487323/1‑94 (MQ=60)
                     ACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTT                                                                          <  SRR4302541.107212/94‑1 (MQ=60)
                     ACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTT                                                                          <  SRR4302541.3797938/94‑1 (MQ=60)
                     ACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTT                                                                          >  SRR4302541.5008689/1‑94 (MQ=60)
                      CCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTG                                                                         <  SRR4302541.4670235/94‑1 (MQ=60)
                      CCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTG                                                                         <  SRR4302541.7007958/94‑1 (MQ=60)
                       CGGTTCGATAGCGGAGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGC                                                                        <  SRR4302541.7888039/94‑1 (MQ=60)
                        GGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCG                                                                       <  SRR4302541.2522756/94‑1 (MQ=60)
                        GGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCG                                                                       <  SRR4302541.4941965/94‑1 (MQ=60)
                        GGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCG                                                                       >  SRR4302541.6968542/1‑94 (MQ=60)
                        GGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCG                                                                       >  SRR4302541.2202153/1‑94 (MQ=60)
                         GTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGG                                                                      >  SRR4302541.3715786/1‑94 (MQ=60)
                         GTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACACGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGG                                                                      <  SRR4302541.6681675/94‑1 (MQ=60)
                           TCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGAC                                                                    <  SRR4302541.816085/94‑1 (MQ=60)
                            CGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACG                                                                   <  SRR4302541.1647897/94‑1 (MQ=60)
                            CGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACG                                                                   <  SRR4302541.4282989/94‑1 (MQ=60)
                             GATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGT                                                                  >  SRR4302541.7775719/1‑94 (MQ=60)
                             GATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGT                                                                  >  SRR4302541.1373995/1‑94 (MQ=60)
                             GATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTACTGTTCGCGCCGCTCTTCAAACTGTACATCTTGCGGACGT                                                                  >  SRR4302541.812819/1‑94 (MQ=60)
                              ATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATTTACTTCTTCCTTTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTA                                                                 >  SRR4302541.6756707/1‑94 (MQ=60)
                               TAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTAC                                                                <  SRR4302541.3905825/94‑1 (MQ=60)
                               TAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTAC                                                                >  SRR4302541.6563341/1‑94 (MQ=60)
                               TAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTAC                                                                >  SRR4302541.7647532/1‑94 (MQ=60)
                                  CGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAAT                                                             <  SRR4302541.2971480/94‑1 (MQ=60)
                                   GGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATT                                                            >  SRR4302541.3437893/1‑94 (MQ=60)
                                   GGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATT                                                            <  SRR4302541.6930549/94‑1 (MQ=60)
                                       GCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGAT                                                        >  SRR4302541.7240166/1‑94 (MQ=60)
                                       GCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGAT                                                        <  SRR4302541.3683201/94‑1 (MQ=60)
                                       GCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGAT                                                        >  SRR4302541.1259055/1‑94 (MQ=60)
                                        CGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATA                                                       <  SRR4302541.6367465/94‑1 (MQ=60)
                                        CGACAGGGACCTCAGTCACCATACGCTCCACATGTACTTCTTCCTGTTCCTGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATA                                                       >  SRR4302541.1244829/1‑94 (MQ=60)
                                         GACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAG                                                      <  SRR4302541.7703764/94‑1 (MQ=60)
                                         GACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAG                                                      <  SRR4302541.1434823/94‑1 (MQ=60)
                                         GACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAG                                                      >  SRR4302541.3789494/1‑94 (MQ=60)
                                          ACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGC                                                     >  SRR4302541.3342078/1‑94 (MQ=60)
                                          ACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGC                                                     >  SRR4302541.7828662/1‑94 (MQ=60)
                                           CAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCG                                                    <  SRR4302541.2032939/94‑1 (MQ=60)
                                           CAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCG                                                    >  SRR4302541.4880633/1‑94 (MQ=60)
                                            AGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGG                                                   >  SRR4302541.5399253/1‑94 (MQ=60)
                                            AGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGG                                                   >  SRR4302541.3350282/1‑94 (MQ=60)
                                              GGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGAT                                                 >  SRR4302541.8110733/1‑94 (MQ=60)
                                              GGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGAT                                                 <  SRR4302541.3235859/94‑1 (MQ=60)
                                               GACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATC                                                >  SRR4302541.96015/1‑94 (MQ=60)
                                                ACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCC                                               >  SRR4302541.7525507/1‑94 (MQ=60)
                                                  CTCAGTCACCATCGGCTGCACCTGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTCACTCTCGCGGCCGTACAATTGGATAGCGGATCCAG                                             >  SRR4302541.8586511/1‑94 (MQ=60)
                                                  CTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAG                                             <  SRR4302541.1629770/94‑1 (MQ=60)
                                                  CTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAG                                             <  SRR4302541.6947694/94‑1 (MQ=60)
                                                  CTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAG                                             <  SRR4302541.6947695/94‑1 (MQ=60)
                                                  CTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAG                                             >  SRR4302541.8586512/1‑94 (MQ=60)
                                                   TCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGA                                            >  SRR4302541.3792946/1‑94 (MQ=60)
                                                    CAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGAC                                           <  SRR4302541.2443468/94‑1 (MQ=60)
                                                     AGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCCTGCGGACGTACAATTGGATAGCGGATCCAGACT                                          >  SRR4302541.8576616/1‑94 (MQ=60)
                                                      GTCCCCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTT                                         <  SRR4302541.6871495/94‑1 (MQ=60)
                                                      GTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTT                                         >  SRR4302541.5518807/1‑94 (MQ=60)
                                                      GTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTT                                         <  SRR4302541.6871494/94‑1 (MQ=60)
                                                      GTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTT                                         <  SRR4302541.918873/94‑1 (MQ=60)
                                                       TCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTT                                        >  SRR4302541.2460628/1‑94 (MQ=60)
                                                        CACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTG                                       <  SRR4302541.7934221/94‑1 (MQ=60)
                                                         CCCATCGGCGGCACATTTACTTCTTTCTGTTCGCCCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGC                                      <  SRR4302541.6814171/94‑1 (MQ=60)
                                                            ATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAG                                   <  SRR4302541.1355224/94‑1 (MQ=60)
                                                            ATCGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAG                                   <  SRR4302541.6770394/94‑1 (MQ=60)
                                                              CGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAG                                 <  SRR4302541.6241032/94‑1 (MQ=60)
                                                              CGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAG                                 >  SRR4302541.1953513/1‑94 (MQ=60)
                                                              CGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAG                                 >  SRR4302541.4818532/1‑94 (MQ=60)
                                                              CGGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAG                                 >  SRR4302541.6678384/1‑94 (MQ=60)
                                                               GGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGG                                >  SRR4302541.2120524/1‑94 (MQ=60)
                                                               GGCTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGG                                >  SRR4302541.5597972/1‑94 (MQ=60)
                                                                 CTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGGCTTTGCCAGAGGCC                              >  SRR4302541.1317767/1‑94 (MQ=60)
                                                                 CTGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCC                              >  SRR4302541.5280035/1‑94 (MQ=60)
                                                                  TGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGTATCCACACTTTGCCAGAGGCCA                             >  SRR4302541.5060118/1‑94 (MQ=60)
                                                                  TGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCA                             <  SRR4302541.725857/94‑1 (MQ=60)
                                                                  TGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCA                             >  SRR4302541.335082/1‑94 (MQ=60)
                                                                  CGCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCA                             <  SRR4302541.1747612/94‑1 (MQ=60)
                                                                   GCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAG                            <  SRR4302541.5268844/94‑1 (MQ=60)
                                                                   GCACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAG                            >  SRR4302541.3102217/1‑94 (MQ=60)
                                                                    CACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGT                           <  SRR4302541.5614005/94‑1 (MQ=60)
                                                                     ACATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTT                          >  SRR4302541.2476969/1‑94 (MQ=60)
                                                                      CATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTC                         >  SRR4302541.8914405/1‑94 (MQ=60)
                                                                      CATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTC                         <  SRR4302541.5827410/94‑1 (MQ=60)
                                                                      CATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTC                         >  SRR4302541.5841813/1‑94 (MQ=60)
                                                                       ATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCC                        <  SRR4302541.8358846/94‑1 (MQ=60)
                                                                       ATGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAACTGGATAGCGGATCCAGAATTTGCCAGAGGCCAGTTCC                        >  SRR4302541.8880967/1‑94 (MQ=60)
                                                                        TGTACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCG                       >  SRR4302541.7263349/1‑94 (MQ=60)
                                                                          TACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGA                     >  SRR4302541.5639347/1‑94 (MQ=60)
                                                                          TACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGA                     >  SRR4302541.5639348/1‑94 (MQ=60)
                                                                           ACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAG                    <  SRR4302541.3420705/94‑1 (MQ=60)
                                                                           ACTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAG                    >  SRR4302541.822190/1‑94 (MQ=60)
                                                                            CTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTTCGGACGTACAATTGGATAGCGTATCCAGACTTTGCCAGAGGCCAGTTCCGGAGA                   >  SRR4302541.4954847/1‑94 (MQ=60)
                                                                            CTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAagtcctgaga                   >  SRR4302541.6473316/1‑84 (MQ=60)
                                                                            CTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGC                   >  SRR4302541.410966/1‑94 (MQ=60)
                                                                            CTTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGGTCCGGAGC                   >  SRR4302541.5974379/1‑94 (MQ=60)
                                                                             TTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGTACGTACAATTGGATAGCGAATCCAGACTTTGCCAGAGGCCACTTCCTGAAAC                  >  SRR4302541.3812675/1‑94 (MQ=60)
                                                                             TTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAC                  <  SRR4302541.185552/94‑1 (MQ=60)
                                                                             TTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAC                  <  SRR4302541.2177015/94‑1 (MQ=60)
                                                                             TTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAC                  <  SRR4302541.8598393/94‑1 (MQ=60)
                                                                             TTCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAC                  >  SRR4302541.3812677/1‑94 (MQ=60)
                                                                              TCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACG                 <  SRR4302541.5583275/94‑1 (MQ=60)
                                                                              TCTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACG                 >  SRR4302541.3574244/1‑94 (MQ=60)
                                                                               CTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGC                >  SRR4302541.7020206/1‑94 (MQ=60)
                                                                               CTTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGC                >  SRR4302541.4126959/1‑94 (MQ=60)
                                                                               CTGCCTGTTCGCGCCGCTCTTCAACTTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTGCCGGAGACGC                <  SRR4302541.5177387/94‑1 (MQ=60)
                                                                                TTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCG               <  SRR4302541.1043777/94‑1 (MQ=60)
                                                                                TTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCG               <  SRR4302541.6692643/94‑1 (MQ=60)
                                                                                TTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCG               >  SRR4302541.1458535/1‑94 (MQ=60)
                                                                                TTCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCC               >  SRR4302541.4660789/1‑94 (MQ=60)
                                                                                 TCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGC              <  SRR4302541.972342/94‑1 (MQ=60)
                                                                                 TCCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGC              >  SRR4302541.1034256/1‑94 (MQ=60)
                                                                                  CCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCA             <  SRR4302541.8469130/94‑1 (MQ=60)
                                                                                  CCTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCA             >  SRR4302541.2868854/1‑94 (MQ=60)
                                                                                   CTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAACGGATCCAGACTTTGCCAGACGCCAGCTCCGGAGACGCCCAC            >  SRR4302541.4441255/1‑94 (MQ=60)
                                                                                   CTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCAC            <  SRR4302541.6996128/94‑1 (MQ=60)
                                                                                   CTGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCAC            <  SRR4302541.6466973/94‑1 (MQ=60)
                                                                                    TGTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACG           <  SRR4302541.5628816/94‑1 (MQ=60)
                                                                                     GTTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGC          >  SRR4302541.3678656/1‑94 (MQ=60)
                                                                                      TTCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCT         <  SRR4302541.6399933/94‑1 (MQ=60)
                                                                                       TCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTA        <  SRR4302541.3718421/94‑1 (MQ=60)
                                                                                       TCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTA        <  SRR4302541.4346559/94‑1 (MQ=60)
                                                                                       TCGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTGTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTA        <  SRR4302541.8401303/94‑1 (MQ=60)
                                                                                        CGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTAC       >  SRR4302541.2196095/1‑94 (MQ=60)
                                                                                        CGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTAC       <  SRR4302541.1533390/94‑1 (MQ=60)
                                                                                        CGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTAC       >  SRR4302541.4460356/1‑94 (MQ=60)
                                                                                        CGCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGAGGATCCAGACTTTGCCAGAGGCCAGTACCGGAGCCGAGCCCGCTAC       >  SRR4302541.7564205/1‑94 (MQ=60)
                                                                                         GCGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACG      <  SRR4302541.2598508/94‑1 (MQ=60)
                                                                                          CGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGG     <  SRR4302541.4265394/94‑1 (MQ=60)
                                                                                          CGCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGG     >  SRR4302541.3989550/1‑94 (MQ=60)
                                                                                           GCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGT    >  SRR4302541.7692269/1‑94 (MQ=60)
                                                                                           GCCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGT    >  SRR4302541.8895173/1‑94 (MQ=60)
                                                                                            CCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTC   <  SRR4302541.637284/94‑1 (MQ=60)
                                                                                            CCGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTC   <  SRR4302541.2631727/94‑1 (MQ=60)
                                                                                             CGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCA  >  SRR4302541.6636112/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCA  >  NC_000913/1140922‑1141108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑