Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,142,917	T→C	D225G (GAC→GGC)	rne ←	RNase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,142,917	0	T	C	98.7%	754.5 / ‑1.8	226	D225G (GAC→GGC)	rne	RNase E
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (135/88); total (136/90)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 236 total aligned reads displayed.

TTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTT  >  NC_000913/1142827‑1143006

ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGccc                                                                                         <  1:4356851/93‑1 (MQ=255)

 tgctgcTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCt                                                                                        <  2:2588616/93‑1 (MQ=255)

  gctgctGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTg                                                                                       >  1:8435809/1‑93 (MQ=255)

  gctgctGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTg                                                                                       >  1:3669365/1‑93 (MQ=255)

   ctgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGa                                                                                      <  2:2061556/93‑1 (MQ=255)

    tgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTTGAGCACTTTCGGGTTATCGATAAGGATCTCGCCGATGACCTGAc                                                                                     >  2:270997/1‑93 (MQ=255)

    tgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGAc                                                                                     <  2:4672444/93‑1 (MQ=255)

    tgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGAc                                                                                     >  1:2242314/1‑93 (MQ=255)

    tgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGAc                                                                                     >  2:7059269/1‑93 (MQ=255)

       tgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgta                                                                                  >  1:5861690/1‑93 (MQ=255)

        gAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaa                                                                                 >  1:8231093/1‑93 (MQ=255)

        gAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaa                                                                                 >  2:2926841/1‑93 (MQ=255)

         aaaTCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaag                                                                                <  2:2230073/93‑1 (MQ=255)

         aaaTCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaag                                                                                >  1:5504327/1‑93 (MQ=255)

          aaTCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaagt                                                                               >  2:1280437/1‑93 (MQ=255)

            tCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaagtaa                                                                             <  1:7898342/93‑1 (MQ=255)

            tCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACgtaagtaa                                                                             <  2:3632768/93‑1 (MQ=255)

              cGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATc                                                                           >  2:5065543/1‑93 (MQ=255)

                   gACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGa                                                                      >  2:5557341/1‑93 (MQ=255)

                   gACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGCTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGa                                                                      >  1:8648490/1‑93 (MQ=255)

                    aCCTAATGGAGGGATAGGCTGGCTTGCCAGTTCGGGGACTTTCGGGTTTTCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGaa                                                                     <  2:8057335/93‑1 (MQ=255)

                    aCCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGaa                                                                     <  2:1008224/93‑1 (MQ=255)

                    aCCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGaa                                                                     >  2:7702778/1‑93 (MQ=255)

                      cTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATg                                                                   >  2:4610831/1‑93 (MQ=255)

                       tAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATgc                                                                  <  2:7166695/93‑1 (MQ=255)

                       tAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATgc                                                                  <  2:6675242/93‑1 (MQ=255)

                       tAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATgc                                                                  >  2:6223273/1‑93 (MQ=255)

                        aaTGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATgcg                                                                 >  2:1948462/1‑93 (MQ=255)

                        aaTGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATgcg                                                                 >  2:353196/1‑93 (MQ=255)

                         aTGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATgcgc                                                                >  1:4632887/1‑93 (MQ=255)

                          tGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGcgcg                                                               <  2:1791053/93‑1 (MQ=255)

                             aGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAAc                                                            <  1:2332381/93‑1 (MQ=255)

                              gCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACg                                                           >  2:7947371/1‑93 (MQ=255)

                                gATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAt                                                         >  1:5811515/1‑93 (MQ=255)

                                gATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAt                                                         >  1:3935744/1‑93 (MQ=255)

                                gATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAt                                                         >  2:4361170/1‑93 (MQ=255)

                                 atatGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt                                                        >  1:2855786/1‑93 (MQ=255)

                                  tatGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa                                                       >  2:6936952/1‑93 (MQ=255)

                                   atGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc                                                      <  1:6817723/93‑1 (MQ=255)

                                   atGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc                                                      >  1:6441980/1‑93 (MQ=255)

                                    tGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATTCGCGCACGAttgct                                                     >  1:947969/1‑90 (MQ=255)

                                    tGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACg                                                     >  1:4897982/1‑93 (MQ=255)

                                     gCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt                                                    <  1:813479/93‑1 (MQ=255)

                                     gCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt                                                    >  2:3356886/1‑93 (MQ=255)

                                     gCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt                                                    <  1:1245413/93‑1 (MQ=255)

                                      cTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGtt                                                   <  1:183650/93‑1 (MQ=255)

                                       tGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTg                                                  >  2:2834734/1‑93 (MQ=255)

                                       tGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTg                                                  >  2:1585992/1‑93 (MQ=255)

                                         aCGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGct                                                >  1:1137495/1‑93 (MQ=255)

                                          cGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGctc                                               >  2:7961496/1‑93 (MQ=255)

                                          cGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGctc                                               >  1:7172915/1‑93 (MQ=255)

                                             gCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTcc                                            >  1:1073365/1‑93 (MQ=255)

                                             gCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTcc                                            <  1:3653545/93‑1 (MQ=255)

                                               cAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtg                                          <  1:6033939/93‑1 (MQ=255)

                                               cAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtg                                          >  1:2545976/1‑93 (MQ=255)

                                               cAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtg                                          >  2:5853904/1‑93 (MQ=255)

                                               cAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtg                                          <  1:4301141/93‑1 (MQ=255)

                                                 gTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgat                                        >  1:848696/1‑93 (MQ=255)

                                                   tCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgatga                                      >  1:4477010/1‑93 (MQ=255)

                                                    cGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGaa                                     >  2:1739933/1‑93 (MQ=255)

                                                     gAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAAt                                    <  2:8575850/93‑1 (MQ=255)

                                                      aGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATc                                   <  1:2099253/93‑1 (MQ=255)

                                                       gCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCa                                  >  1:4007643/1‑93 (MQ=255)

                                                        cACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAg                                 >  1:7722605/1‑93 (MQ=255)

                                                          cTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGa                               >  2:3161761/1‑93 (MQ=255)

                                                            ttCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAAc                             <  2:1540655/93‑1 (MQ=255)

                                                            ttCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAAc                             <  1:5342275/93‑1 (MQ=255)

                                                            ttCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAAc                             <  2:5511580/93‑1 (MQ=255)

                                                             tCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACg                            <  2:8022091/93‑1 (MQ=255)

                                                               gggTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACggg                          <  2:7415444/93‑1 (MQ=255)

                                                                ggTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACgggg                         >  2:2949951/1‑93 (MQ=255)

                                                                ggTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACgggg                         >  1:7549232/1‑93 (MQ=255)

                                                                ggTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACgggg                         >  2:3104703/1‑93 (MQ=255)

                                                                 gTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGc                        <  2:3868748/93‑1 (MQ=255)

                                                                   tATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCg                      <  1:5586879/93‑1 (MQ=255)

                                                                    aTCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCgg                     >  1:6257873/1‑93 (MQ=255)

                                                                       gATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcg                  >  1:1712872/1‑93 (MQ=255)

                                                                       gATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcg                  <  2:5937997/93‑1 (MQ=255)

                                                                        aTAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcgg                 >  1:8396767/1‑93 (MQ=255)

                                                                         tAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcggc                >  2:5206041/1‑93 (MQ=255)

                                                                         tAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcggc                >  1:2775594/1‑93 (MQ=255)

                                                                          aaGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCGCTCCTGATGAATCAGGAACGGGGCCGGGCGGCt               <  1:5725004/93‑1 (MQ=255)

                                                                            ggATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCttt             >  2:6495014/1‑93 (MQ=255)

                                                                            ggATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCttt             >  1:4783410/1‑93 (MQ=255)

                                                                             gATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTc            <  2:5167146/93‑1 (MQ=255)

                                                                             gATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTc            <  1:1529527/93‑1 (MQ=255)

                                                                              aTTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCa           <  1:6987637/93‑1 (MQ=255)

                                                                                    ccGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCt     >  2:4138293/1‑93 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   <  2:8256854/93‑1 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   >  1:3041041/1‑93 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   <  2:5616989/93‑1 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   >  1:3435306/1‑93 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   >  1:5246809/1‑93 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   >  2:7618867/1‑93 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   >  2:7764466/1‑93 (MQ=255)

                                                                                      gATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt   >  1:8666466/1‑93 (MQ=255)

                                                                                       aTGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttt  >  1:8518945/1‑93 (MQ=255)

                                                                                       aTGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttt  >  2:7332169/1‑93 (MQ=255)

                                                                                       aTGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttt  >  1:3357335/1‑93 (MQ=255)

                                                                                       aTGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttt  <  1:7117583/93‑1 (MQ=255)

TTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTT  >  NC_000913/1142827‑1143006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGA  >  NC_000913/1142824‑1143010

ATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGC                                                                                               <  SRR4302541.6537324/94‑1 (MQ=60)

   TTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCT                                                                                            <  SRR4302541.4400375/94‑1 (MQ=60)

    TGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTG                                                                                           >  SRR4302541.3706573/1‑94 (MQ=60)

    TGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTG                                                                                           >  SRR4302541.150334/1‑94 (MQ=60)

    TGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTG                                                                                           >  SRR4302541.8526939/1‑94 (MQ=60)

      CTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGAC                                                                                         >  SRR4302541.2264024/1‑94 (MQ=60)

      CTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGAC                                                                                         <  SRR4302541.6423373/94‑1 (MQ=60)

      CTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGAC                                                                                         <  SRR4302541.7841816/94‑1 (MQ=60)

       TGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACG                                                                                        >  SRR4302541.237711/1‑94 (MQ=60)

       TGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACG                                                                                        >  SRR4302541.2287695/1‑94 (MQ=60)

       TGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACG                                                                                        >  SRR4302541.3772518/1‑94 (MQ=60)

         CTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTA                                                                                      >  SRR4302541.5921310/1‑94 (MQ=60)

          TGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAA                                                                                     >  SRR4302541.8320822/1‑94 (MQ=60)

           GAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAG                                                                                    >  SRR4302541.5560705/1‑94 (MQ=60)

            AAATCCGGGCGACCTAATGGAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGT                                                                                   <  SRR4302541.4966300/94‑1 (MQ=60)

               TCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAAT                                                                                <  SRR4302541.7985212/94‑1 (MQ=60)

                CCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATC                                                                               <  SRR4302541.8407606/94‑1 (MQ=60)

                 CGGGAGAACTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACGTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCG                                                                              <  SRR4302541.1856654/94‑1 (MQ=60)

                  GGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGC                                                                             >  SRR4302541.6128808/1‑94 (MQ=60)

                     ggaacagATGCAGCGATATGCTGGCGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGA                                                                          <  SRR4302541.2704894/87‑1 (MQ=60)

                     CGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTGGCCGATGCCCTGACGTAAGTAATCGCGGA                                                                          >  SRR4302541.1105160/1‑94 (MQ=60)

                     CGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGCTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGA                                                                          >  SRR4302541.8741099/1‑94 (MQ=60)

                      GACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAA                                                                         >  SRR4302541.3842395/1‑94 (MQ=60)

                      GACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGCAA                                                                         >  SRR4302541.760744/1‑94 (MQ=60)

                       ACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAT                                                                        <  SRR4302541.8139665/94‑1 (MQ=60)

                       ACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAAT                                                                        <  SRR4302541.3449962/94‑1 (MQ=60)

                        CCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATG                                                                       <  SRR4302541.3478332/94‑1 (MQ=60)

                        CCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGACGATGCCCTGACGTAAGTAATCGCGGAATG                                                                       >  SRR4302541.3462355/1‑94 (MQ=60)

                         CTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGC                                                                      >  SRR4302541.5627788/1‑94 (MQ=60)

                           cctcgggcgatatcggttgtcgccagttgcagcagTTTCGGGTGATCGATAAGGATTTCGCCGATGGCCTGACGTAAGTAATCGCGGAATGCGC                                                                    <  SRR4302541.4884906/59‑1 (MQ=60)

                           AATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGC                                                                    >  SRR4302541.4680030/1‑94 (MQ=60)

                            ATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCG                                                                   <  SRR4302541.7203821/94‑1 (MQ=60)

                             TGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGA                                                                  <  SRR4302541.4336923/94‑1 (MQ=60)

                                AGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAGTCGCGGAATGCGCGAACG                                                               <  SRR4302541.2831027/94‑1 (MQ=60)

                                AGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACG                                                               <  SRR4302541.2355028/94‑1 (MQ=60)

                                AGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACG                                                               <  SRR4302541.6342499/94‑1 (MQ=60)

                                AGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACG                                                               >  SRR4302541.4631928/1‑94 (MQ=60)

                                  CGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAT                                                             >  SRR4302541.6918406/1‑94 (MQ=60)

                                  CGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAT                                                             >  SRR4302541.5870560/1‑94 (MQ=60)

                                  CGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGAT                                                             >  SRR4302541.3974929/1‑94 (MQ=60)

                                   NANANGNNGNCGTGCCAGTTCNNGCGCTTTCGGGTTATCGATAAGGATTTCNCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATT                                                            <  SRR4302541.5342298/94‑1 (MQ=60)

                                   GATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATT                                                            <  SRR4302541.1343139/94‑1 (MQ=60)

                                   GATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATT                                                            >  SRR4302541.2884494/1‑94 (MQ=60)

                                     TATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAC                                                          >  SRR4302541.6507643/1‑94 (MQ=60)

                                      ATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATTCGCGCACGATTgct                                                         >  SRR4302541.956974/1‑91 (MQ=60)

                                      ATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACG                                                         >  SRR4302541.4947959/1‑94 (MQ=60)

                                      ATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACG                                                         <  SRR4302541.6890681/94‑1 (MQ=60)

                                       TGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGT                                                        >  SRR4302541.8153717/1‑94 (MQ=60)

                                        GCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTT                                                       <  SRR4302541.820911/94‑1 (MQ=60)

                                        GCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTT                                                       <  SRR4302541.1257757/94‑1 (MQ=60)

                                         CTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTG                                                      <  SRR4302541.185027/94‑1 (MQ=60)

                                           GACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCT                                                    <  SRR4302541.5880170/94‑1 (MQ=60)

                                           GACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCT                                                    >  SRR4302541.1148293/1‑94 (MQ=60)

                                            ACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTC                                                   >  SRR4302541.7249391/1‑94 (MQ=60)

                                             CGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTGGCCGATTCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCT                                                  <  SRR4302541.1659164/94‑1 (MQ=60)

                                             CGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCT                                                  >  SRR4302541.7599330/1‑94 (MQ=60)

                                             CGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCT                                                  <  SRR4302541.1974451/94‑1 (MQ=60)

                                             CGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCT                                                  >  SRR4302541.8594361/1‑94 (MQ=60)

                                               TGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCC                                                >  SRR4302541.1083363/1‑94 (MQ=60)

                                                GCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCT                                               <  SRR4302541.3690612/94‑1 (MQ=60)

                                                 CCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTG                                              >  SRR4302541.2571518/1‑94 (MQ=60)

                                                  CAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGA                                             <  SRR4302541.6095721/94‑1 (MQ=60)

                                                  CAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGA                                             >  SRR4302541.965937/1‑94 (MQ=60)

                                                  CAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGA                                             <  SRR4302541.4344095/94‑1 (MQ=60)

                                                   AGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCTTGAT                                            >  SRR4302541.2799921/1‑94 (MQ=60)

                                                   AGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGAT                                            >  SRR4302541.5758252/1‑94 (MQ=60)

                                                   AGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGAT                                            >  SRR4302541.856415/1‑94 (MQ=60)

                                                   AGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGAT                                            >  SRR4302541.856417/1‑94 (MQ=60)

                                                    GTTCGAGCACTTTCGGGTTATCGATAATGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATG                                           <  SRR4302541.2976908/94‑1 (MQ=60)

                                                     TTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGA                                          >  SRR4302541.4522123/1‑94 (MQ=60)

                                                      TCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAA                                         >  SRR4302541.8906414/1‑94 (MQ=60)

                                                       CGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGCACGATTACGTTGCTCTCCTGATGAAC                                        >  SRR4302541.4926967/1‑94 (MQ=60)

                                                        GAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATC                                       >  SRR4302541.523592/1‑94 (MQ=60)

                                                        GAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAATAATCGCGGAATGCGCGAACCATTAAGTTGCTCTCCTGATGAATC                                       <  SRR4302541.4176521/94‑1 (MQ=60)

                                                         AGCGCTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCA                                      <  SRR4302541.3986608/94‑1 (MQ=60)

                                                         AGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCA                                      >  SRR4302541.1832155/1‑94 (MQ=60)

                                                         AGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCA                                      >  SRR4302541.4047290/1‑94 (MQ=60)

                                                         AGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCA                                      <  SRR4302541.2119594/94‑1 (MQ=60)

                                                          GCACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAG                                     >  SRR4302541.7804756/1‑94 (MQ=60)

                                                            ACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGA                                   >  SRR4302541.2981448/1‑94 (MQ=60)

                                                            ACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGA                                   >  SRR4302541.5853591/1‑94 (MQ=60)

                                                            ACTTTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGA                                   >  SRR4302541.3702449/1‑94 (MQ=60)

                                                               TTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACG                                >  SRR4302541.532024/1‑94 (MQ=60)

                                                               TTCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACG                                <  SRR4302541.5397190/94‑1 (MQ=60)

                                                                TCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGG                               <  SRR4302541.1227778/94‑1 (MQ=60)

                                                                TCGGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGG                               <  SRR4302541.4758368/94‑1 (MQ=60)

                                                                  GGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGG                             >  SRR4302541.4346475/1‑94 (MQ=60)

                                                                  GGGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGG                             >  SRR4302541.7629908/1‑94 (MQ=60)

                                                                   GGTTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGC                            >  SRR4302541.3238468/1‑94 (MQ=60)

                                                                     TTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCG                          <  SRR4302541.8252111/94‑1 (MQ=60)

                                                                     TTATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCG                          >  SRR4302541.6029849/1‑94 (MQ=60)

                                                                      TATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGG                         >  SRR4302541.6321794/1‑94 (MQ=60)

                                                                      TATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGG                         <  SRR4302541.5644072/94‑1 (MQ=60)

                                                                       ATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGG                        >  SRR4302541.4733546/1‑94 (MQ=60)

                                                                       ATCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGG                        >  SRR4302541.8681050/1‑94 (MQ=60)

                                                                        TCGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGC                       >  SRR4302541.4430872/1‑94 (MQ=60)

                                                                         CGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCG                      >  SRR4302541.7711707/1‑94 (MQ=60)

                                                                         CGATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCG                      >  SRR4302541.1729639/1‑94 (MQ=60)

                                                                          GATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGG                     >  SRR4302541.8880884/1‑94 (MQ=60)

                                                                          GATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGG                     >  SRR4302541.8487641/1‑94 (MQ=60)

                                                                          GATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATCACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGG                     >  SRR4302541.8755945/1‑94 (MQ=60)

                                                                           ATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGC                    >  SRR4302541.7169725/1‑94 (MQ=60)

                                                                           ATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGC                    >  SRR4302541.5551069/1‑94 (MQ=60)

                                                                           ATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGC                    >  SRR4302541.2803557/1‑94 (MQ=60)

                                                                           ATAAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTATCCTGATGAATCAGGAACGGGGCCGGGGGGC                    >  SRR4302541.8487674/1‑94 (MQ=60)

                                                                             AAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTT                  <  SRR4302541.7082365/94‑1 (MQ=60)

                                                                             AAGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCGCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTT                  <  SRR4302541.5783367/94‑1 (MQ=60)

                                                                              AGGATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTT                 >  SRR4302541.4832232/1‑94 (MQ=60)

                                                                                GATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCA               <  SRR4302541.1544835/94‑1 (MQ=60)

                                                                                 ATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAG              >  SRR4302541.2137427/1‑94 (MQ=60)

                                                                                 ATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAG              <  SRR4302541.8629978/94‑1 (MQ=60)

                                                                                 ATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAG              <  SRR4302541.7062048/94‑1 (MQ=60)

                                                                                 ATTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAG              <  SRR4302541.5639167/94‑1 (MQ=60)

                                                                                  TTTCGCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGC             <  SRR4302541.6735823/94‑1 (MQ=60)

                                                                                   TTCGCCGATGCCCTGACGTAAGTAATCCCGGAATGCGCGAACGATTACGTTGCTCTCCTGATTAATCAGGAACGGGGCCGGGCGGCTTTCAGCG            <  SRR4302541.4357993/94‑1 (MQ=60)

                                                                                      GCCGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCT         >  SRR4302541.2357035/1‑94 (MQ=60)

                                                                                        CGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTT       >  SRR4302541.5300870/1‑94 (MQ=60)

                                                                                        CGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTT       >  SRR4302541.8759226/1‑94 (MQ=60)

                                                                                        CGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTT       >  SRR4302541.84948/1‑94 (MQ=60)

                                                                                        CGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTT       >  SRR4302541.3071772/1‑94 (MQ=60)

                                                                                        CGATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTT       >  SRR4302541.3469625/1‑94 (MQ=60)

                                                                                         GATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTT      >  SRR4302541.8610631/1‑94 (MQ=60)

                                                                                         GATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTT      <  SRR4302541.588609/94‑1 (MQ=60)

                                                                                         GATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTT      >  SRR4302541.3390837/1‑94 (MQ=60)

                                                                                          ATGCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTT     <  SRR4302541.7193392/94‑1 (MQ=60)

                                                                                            GCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTG   <  SRR4302541.8420042/94‑1 (MQ=60)

                                                                                            GCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTG   >  SRR4302541.4430000/1‑94 (MQ=60)

                                                                                            GCCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTG   >  SRR4302541.490088/1‑94 (MQ=60)

                                                                                             CCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGA  >  SRR4302541.3625124/1‑94 (MQ=60)

ATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGA  >  NC_000913/1142824‑1143010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`