Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,143,101 T→C M164V (ATG→GTG)  rne ← RNase E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,1010TC99.6% 854.0 / ‑4.5 254M164V (ATG→GTG) rneRNase E
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (146/107);  total (147/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

Only 100 of 260 total aligned reads displayed.

TGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCG  >  NC_000913/1143011‑1143190
                                                                                          |                                                                                         
 ggCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGcc                                                                                        <  2:3152896/93‑1 (MQ=255)
 ggCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGcc                                                                                        >  1:6176968/1‑93 (MQ=255)
  gCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCt                                                                                       >  2:8051887/1‑93 (MQ=255)
      cccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCg                                                                                   >  2:912619/1‑93 (MQ=255)
      cccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCg                                                                                   >  2:4338974/1‑93 (MQ=255)
       ccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCgg                                                                                  >  2:4618613/1‑93 (MQ=255)
       ccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCgg                                                                                  <  1:3356886/93‑1 (MQ=255)
       ccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCgg                                                                                  >  1:2899074/1‑93 (MQ=255)
        cAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGc                                                                                 <  2:6845215/93‑1 (MQ=255)
        cAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGc                                                                                 >  2:2233337/1‑93 (MQ=255)
        cAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGc                                                                                 <  1:8695329/93‑1 (MQ=255)
          gtgtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAg                                                                               >  1:5798934/1‑93 (MQ=255)
           tgtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGt                                                                              <  2:1633702/93‑1 (MQ=255)
            gtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGtt                                                                             >  1:2752618/1‑93 (MQ=255)
            gtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGtt                                                                             <  2:7981959/93‑1 (MQ=255)
             tttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTc                                                                            >  1:5933351/1‑93 (MQ=255)
             tttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTc                                                                            >  1:5229434/1‑93 (MQ=255)
               tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                          <  2:3665263/93‑1 (MQ=255)
               tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                          <  1:3193021/93‑1 (MQ=255)
               tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                          >  1:8156970/1‑93 (MQ=255)
               tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                          <  2:5967625/93‑1 (MQ=255)
               tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                          >  2:6001403/1‑93 (MQ=255)
                cAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAg                                                                         >  1:3629771/1‑93 (MQ=255)
                 aGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAgg                                                                        >  2:8274117/1‑93 (MQ=255)
                  gACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGc                                                                       <  2:1485060/93‑1 (MQ=255)
                  gACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGc                                                                       >  1:5177117/1‑93 (MQ=255)
                  gACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGc                                                                       <  2:8435809/93‑1 (MQ=255)
                  gACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGc                                                                       >  2:8535003/1‑93 (MQ=255)
                  aaCGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGc                                                                       <  1:3206922/92‑1 (MQ=255)
                   aCGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCt                                                                      >  1:4761409/1‑93 (MQ=255)
                   aCGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCt                                                                      >  1:3263962/1‑93 (MQ=255)
                    cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCtt                                                                     <  1:8823512/93‑1 (MQ=255)
                    cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCtt                                                                     >  2:7849329/1‑93 (MQ=255)
                    cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCtt                                                                     <  1:2276538/93‑1 (MQ=255)
                     ggAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTg                                                                    >  2:3631972/1‑93 (MQ=255)
                     ggAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTg                                                                    >  2:278999/1‑93 (MQ=255)
                      gAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGc                                                                   <  1:311185/93‑1 (MQ=255)
                       aaGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGcc                                                                  <  2:3741214/93‑1 (MQ=255)
                        aGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCa                                                                 <  2:5570756/93‑1 (MQ=255)
                         gCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAg                                                                <  2:446560/93‑1 (MQ=255)
                         gCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAg                                                                <  2:3428118/93‑1 (MQ=255)
                            tAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGTTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGc                                                             <  1:7947371/93‑1 (MQ=255)
                            tAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGc                                                             >  1:5641692/1‑93 (MQ=255)
                             aaaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGct                                                            >  2:3833882/1‑93 (MQ=255)
                               agcccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGcttc                                                          <  2:1433062/91‑1 (MQ=255)
                                tCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGcttct                                                         >  1:7875701/1‑93 (MQ=255)
                                    aTTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTa                                                     >  2:3082243/1‑93 (MQ=255)
                                      tGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAAt                                                   <  1:5320145/93‑1 (MQ=255)
                                      tGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAAt                                                   <  1:2943059/93‑1 (MQ=255)
                                        cAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTc                                                 <  2:7406917/93‑1 (MQ=255)
                                         aGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCg                                                >  2:1920631/1‑93 (MQ=255)
                                          gcgcCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCgt                                               >  1:6695764/1‑92 (MQ=255)
                                          gcgcCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCgg                                               >  2:2863811/1‑93 (MQ=255)
                                            gcCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTa                                             <  1:400018/93‑1 (MQ=255)
                                             ccTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAc                                            <  2:6397897/93‑1 (MQ=255)
                                             ccTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAc                                            >  1:1496093/1‑93 (MQ=255)
                                              cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                           <  1:1782485/93‑1 (MQ=255)
                                              cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                           <  2:7172915/93‑1 (MQ=255)
                                              cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                           <  1:7557805/93‑1 (MQ=255)
                                               tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACgg                                          >  2:5722463/1‑93 (MQ=255)
                                               tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACgg                                          >  1:1638647/1‑93 (MQ=255)
                                               tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACgg                                          >  2:5445212/1‑93 (MQ=255)
                                                cagcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgt                                         <  1:6652691/93‑1 (MQ=255)
                                                 agcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtc                                        <  2:235853/93‑1 (MQ=255)
                                                 agcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtc                                        <  2:2130491/93‑1 (MQ=255)
                                                   cagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgt                                      >  2:3726/1‑93 (MQ=255)
                                                   cagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgt                                      >  2:6495263/1‑93 (MQ=255)
                                                    agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc                                     <  2:7674977/93‑1 (MQ=255)
                                                      aTTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGc                                   >  1:8496623/1‑93 (MQ=255)
                                                         tGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCtt                                >  1:7098046/1‑93 (MQ=255)
                                                         tGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCtt                                >  2:7910759/1‑93 (MQ=255)
                                                            cGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa                             >  2:5040562/1‑93 (MQ=255)
                                                             gACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAt                            >  1:4049836/1‑93 (MQ=255)
                                                              aCGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATa                           <  2:4897982/93‑1 (MQ=255)
                                                              aCGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATa                           <  2:7192200/93‑1 (MQ=255)
                                                               cGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATAc                          >  1:1770029/1‑93 (MQ=255)
                                                                gCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACg                         >  1:434796/1‑93 (MQ=255)
                                                                   aGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCg                      >  2:4212619/1‑93 (MQ=255)
                                                                    gCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCga                     >  2:5835749/1‑93 (MQ=255)
                                                                      ggTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgaga                   <  1:1293662/93‑1 (MQ=255)
                                                                       gTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa                  >  1:2552981/1‑93 (MQ=255)
                                                                       gTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa                  >  1:1004706/1‑93 (MQ=255)
                                                                        tGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaaa                 >  1:7034016/1‑93 (MQ=255)
                                                                        tGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaaa                 >  2:5625386/1‑93 (MQ=255)
                                                                         gcgcACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAAt                <  1:2077572/93‑1 (MQ=255)
                                                                          cgcACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATg               >  2:7073505/1‑93 (MQ=255)
                                                                            taCGATACGCCCCACGCCTTCTGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGcc             <  2:5120685/92‑1 (MQ=255)
                                                                             aCGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCa            >  2:8227341/1‑93 (MQ=255)
                                                                               gATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAcc          >  1:8375978/1‑93 (MQ=255)
                                                                               aaTAAGCCCCACGCCTTCCGGAAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAcc          <  2:8648490/92‑1 (MQ=255)
                                                                                aTAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAccc         >  2:3976226/1‑93 (MQ=255)
                                                                                aTAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAccc         <  2:1140628/93‑1 (MQ=255)
                                                                                aTAAGCCCAACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAccc         >  1:4983032/1‑93 (MQ=255)
                                                                                 tAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcg        <  1:5575951/93‑1 (MQ=255)
                                                                                  aaGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgc       <  2:5504327/93‑1 (MQ=255)
                                                                                  aaGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgc       >  1:3575714/1‑93 (MQ=255)
                                                                                    gCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgc     >  1:3736182/1‑93 (MQ=255)
                                                                                      cccACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgc   >  1:2771459/1‑93 (MQ=255)
                                                                                       ccACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgcg  >  1:1651888/1‑93 (MQ=255)
                                                                                       ccACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgcg  >  2:6147675/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCG  >  NC_000913/1143011‑1143190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTT  >  NC_000913/1143008‑1143194
                                                                                             |                                                                                             
TGATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCAC                                                                                               <  SRR4302541.5932016/94‑1 (MQ=60)
TGATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCAC                                                                                               <  SRR4302541.8332321/94‑1 (MQ=60)
   TGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCC                                                                                            >  SRR4302541.6240072/1‑94 (MQ=60)
     GCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTT                                                                                          <  SRR4302541.8902065/94‑1 (MQ=60)
     GCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTT                                                                                          >  SRR4302541.5715154/1‑94 (MQ=60)
      CTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTC                                                                                         >  SRR4302541.6215160/1‑94 (MQ=60)
        TCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCG                                                                                       <  SRR4302541.1057248/94‑1 (MQ=60)
        TCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCG                                                                                       >  SRR4302541.7179381/1‑94 (MQ=60)
         CCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGG                                                                                      >  SRR4302541.2928202/1‑94 (MQ=60)
          CCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGC                                                                                     <  SRR4302541.3390388/94‑1 (MQ=60)
          CCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGC                                                                                     >  SRR4302541.3212029/1‑94 (MQ=60)
           CAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCA                                                                                    <  SRR4302541.5984863/94‑1 (MQ=60)
           CAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCA                                                                                    <  SRR4302541.8788398/94‑1 (MQ=60)
            AGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAG                                                                                   >  SRR4302541.5857838/1‑94 (MQ=60)
             GTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGT                                                                                  <  SRR4302541.8479138/94‑1 (MQ=60)
              TGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTT                                                                                 >  SRR4302541.2780271/1‑94 (MQ=60)
               GTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTC                                                                                >  SRR4302541.5283320/1‑94 (MQ=60)
               GTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTC                                                                                >  SRR4302541.5993790/1‑94 (MQ=60)
                 TTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAA                                                                              >  SRR4302541.8246111/1‑94 (MQ=60)
                 TTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAA                                                                              >  SRR4302541.345507/1‑94 (MQ=60)
                 TTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAA                                                                              <  SRR4302541.5117617/94‑1 (MQ=60)
                  TCAGGCGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAG                                                                             <  SRR4302541.3225277/94‑1 (MQ=60)
                  TCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAG                                                                             <  SRR4302541.3225276/94‑1 (MQ=60)
                  TCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAG                                                                             <  SRR4302541.2834894/94‑1 (MQ=60)
                  TCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAG                                                                             >  SRR4302541.3666568/1‑94 (MQ=60)
                   CAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGG                                                                            <  SRR4302541.6500905/94‑1 (MQ=60)
                    AGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGC                                                                           >  SRR4302541.1425460/1‑94 (MQ=60)
                    AGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGC                                                                           >  SRR4302541.5230378/1‑94 (MQ=60)
                    AGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGC                                                                           >  SRR4302541.8018385/1‑94 (MQ=60)
                    AGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGC                                                                           >  SRR4302541.5698746/1‑94 (MQ=60)
                     GACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCT                                                                          >  SRR4302541.4810036/1‑94 (MQ=60)
                     GACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCT                                                                          >  SRR4302541.3296893/1‑94 (MQ=60)
                     AACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCT                                                                          <  SRR4302541.3239318/94‑1 (MQ=60)
                      ACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTT                                                                         <  SRR4302541.1624024/94‑1 (MQ=60)
                       CGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTG                                                                        <  SRR4302541.8917670/94‑1 (MQ=60)
                       CGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTG                                                                        <  SRR4302541.2298553/94‑1 (MQ=60)
                        GGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGC                                                                       >  SRR4302541.3247686/1‑94 (MQ=60)
                         GAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCC                                                                      <  SRR4302541.313946/94‑1 (MQ=60)
                          AAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCG                                                                     >  SRR4302541.2897771/1‑94 (MQ=60)
                           AGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAG                                                                    <  SRR4302541.7074141/94‑1 (MQ=60)
                             CTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTG                                                                  >  SRR4302541.7790454/1‑94 (MQ=60)
                              TTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGC                                                                 >  SRR4302541.5699363/1‑94 (MQ=60)
                               TAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGTTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCT                                                                <  SRR4302541.8034919/94‑1 (MQ=60)
                                AAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTT                                                               <  SRR4302541.2967587/94‑1 (MQ=60)
                                  ATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCT                                                             >  SRR4302541.7962235/1‑94 (MQ=60)
                                  ATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCT                                                             >  SRR4302541.89585/1‑94 (MQ=60)
                                   TCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTT                                                            >  SRR4302541.2595760/1‑94 (MQ=60)
                                   TCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTT                                                            <  SRR4302541.2670470/94‑1 (MQ=60)
                                    CCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTT                                                           >  SRR4302541.8669652/1‑94 (MQ=60)
                                    CCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTT                                                           <  SRR4302541.2143473/94‑1 (MQ=60)
                                     CCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTT                                                          >  SRR4302541.4676053/1‑94 (MQ=60)
                                      CATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTA                                                         >  SRR4302541.696368/1‑94 (MQ=60)
                                      CATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTA                                                         >  SRR4302541.39390/1‑94 (MQ=60)
                                       ATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAA                                                        >  SRR4302541.3089348/1‑94 (MQ=60)
                                         TGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATT                                                      <  SRR4302541.5374906/94‑1 (MQ=60)
                                         TGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATT                                                      >  SRR4302541.3169592/1‑94 (MQ=60)
                                         TGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATT                                                      <  SRR4302541.2972656/94‑1 (MQ=60)
                                            AGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGT                                                   >  SRR4302541.6765601/1‑94 (MQ=60)
                                              CGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTA                                                 >  SRR4302541.8668413/1‑94 (MQ=60)
                                              CGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTA                                                 >  SRR4302541.5114254/1‑94 (MQ=60)
                                               GCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAC                                                <  SRR4302541.403721/94‑1 (MQ=60)
                                               GCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAC                                                >  SRR4302541.1762654/1‑94 (MQ=60)
                                               GCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAC                                                >  SRR4302541.4346499/1‑94 (MQ=60)
                                               GCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAC                                                >  SRR4302541.2017929/1‑94 (MQ=60)
                                               GCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAC                                                >  SRR4302541.1510868/1‑94 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              <  SRR4302541.1799788/94‑1 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              >  SRR4302541.3266811/1‑94 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              >  SRR4302541.673236/1‑94 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              >  SRR4302541.1654858/1‑94 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              <  SRR4302541.7638570/94‑1 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              <  SRR4302541.573203/94‑1 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              >  SRR4302541.1345590/1‑94 (MQ=60)
                                                 CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGG                                              >  SRR4302541.406704/1‑94 (MQ=60)
                                                  TCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGT                                             >  SRR4302541.3930940/1‑94 (MQ=60)
                                                  TCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGT                                             <  SRR4302541.8063147/94‑1 (MQ=60)
                                                  TAAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGT                                             <  SRR4302541.7856921/94‑1 (MQ=60)
                                                   CAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTC                                            <  SRR4302541.6720945/94‑1 (MQ=60)
                                                     GCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGT                                          >  SRR4302541.1663045/1‑94 (MQ=60)
                                                        GATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGC                                       >  SRR4302541.8588152/1‑94 (MQ=60)
                                                        GATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGC                                       >  SRR4302541.3037422/1‑94 (MQ=60)
                                                         ATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCC                                      <  SRR4302541.3361593/94‑1 (MQ=60)
                                                           TTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTTCTTCTTTTAATTCGGTACGGTCGTCGCCTT                                    <  SRR4302541.7134345/94‑1 (MQ=60)
                                                           TTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTT                                    <  SRR4302541.5272406/94‑1 (MQ=60)
                                                           TTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTT                                    >  SRR4302541.7173572/1‑94 (MQ=60)
                                                               CGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCTAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAT                                >  SRR4302541.958577/1‑94 (MQ=60)
                                                               CGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAT                                >  SRR4302541.760823/1‑94 (MQ=60)
                                                               CGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAT                                >  SRR4302541.4089798/1‑94 (MQ=60)
                                                               CGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAT                                <  SRR4302541.5581777/94‑1 (MQ=60)
                                                                GACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATC                               >  SRR4302541.2498159/1‑94 (MQ=60)
                                                                GACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATA                               <  SRR4302541.8309503/94‑1 (MQ=60)
                                                                 ACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATAC                              >  SRR4302541.1787238/1‑94 (MQ=60)
                                                                  CGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACG                             <  SRR4302541.600011/94‑1 (MQ=60)
                                                                  CGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACG                             >  SRR4302541.438888/1‑94 (MQ=60)
                                                                  CGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACG                             <  SRR4302541.4657661/94‑1 (MQ=60)
                                                                   GCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGG                            <  SRR4302541.4987866/94‑1 (MQ=60)
                                                                   GCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGG                            >  SRR4302541.8061391/1‑94 (MQ=60)
                                                                    CCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGC                           <  SRR4302541.3740739/94‑1 (MQ=60)
                                                                     CAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCG                          <  SRR4302541.8520808/94‑1 (MQ=60)
                                                                      AGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGA                         >  SRR4302541.3656887/1‑94 (MQ=60)
                                                                       GCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAG                        <  SRR4302541.6698745/94‑1 (MQ=60)
                                                                        CGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTAAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGA                       <  SRR4302541.5495/94‑1 (MQ=60)
                                                                         GGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAA                      >  SRR4302541.2578579/1‑94 (MQ=60)
                                                                         GGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAA                      >  SRR4302541.5342784/1‑94 (MQ=60)
                                                                         GGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAA                      >  SRR4302541.1014035/1‑94 (MQ=60)
                                                                         GGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAA                      <  SRR4302541.1756929/94‑1 (MQ=60)
                                                                         GGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAA                      <  SRR4302541.1306430/94‑1 (MQ=60)
                                                                          GTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAA                     >  SRR4302541.7108789/1‑94 (MQ=60)
                                                                            GCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATG                   <  SRR4302541.2097670/94‑1 (MQ=60)
                                                                             CGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGC                  <  SRR4302541.4159628/94‑1 (MQ=60)
                                                                                ACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAC               >  SRR4302541.1659470/1‑94 (MQ=60)
                                                                                ACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAC               >  SRR4302541.2386113/1‑94 (MQ=60)
                                                                                 CGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACC              >  SRR4302541.8466729/1‑94 (MQ=60)
                                                                                  GATAAGCCCAACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCC             >  SRR4302541.5034146/1‑94 (MQ=60)
                                                                                   ATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCG            <  SRR4302541.356373/94‑1 (MQ=60)
                                                                                    TAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGC           >  SRR4302541.3611945/1‑94 (MQ=60)
                                                                                    TAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGC           <  SRR4302541.5633030/94‑1 (MQ=60)
                                                                                     AAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCG          <  SRR4302541.1881528/94‑1 (MQ=60)
                                                                                      AGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGC         >  SRR4302541.3773790/1‑94 (MQ=60)
                                                                                        CCCCACGCCTTCCGGCAGTTCAAGGGTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTGGCCTTTGTTACGGCGAGAAATACCACCCGCGCGC       >  SRR4302541.2799367/1‑94 (MQ=60)
                                                                                        CCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGC       >  SRR4302541.2799369/1‑94 (MQ=60)
                                                                                         CCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCG      >  SRR4302541.1668203/1‑94 (MQ=60)
                                                                                         CCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCG      >  SRR4302541.5357471/1‑94 (MQ=60)
                                                                                         CCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCG      >  SRR4302541.1790159/1‑94 (MQ=60)
                                                                                          CCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGG     <  SRR4302541.3654448/94‑1 (MQ=60)
                                                                                           CACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGG    >  SRR4302541.8166217/1‑94 (MQ=60)
                                                                                            ACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCCCCCGCGCGCGGGT   >  SRR4302541.8800932/1‑94 (MQ=60)
                                                                                            ACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGT   >  SRR4302541.8801070/1‑94 (MQ=60)
                                                                                             CGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTT  <  SRR4302541.6857903/94‑1 (MQ=60)
                                                                                             CGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGGCGTCGCCTTCGATACGGCGCGAAATGCCACCCcccgcggggtg  >  SRR4302541.7123532/1‑83 (MQ=60)
                                                                                             |                                                                                             
TGATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTT  >  NC_000913/1143008‑1143194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑