Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,154,920 C→T P192S (CCG→TCG)  tmk → thymidylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,154,9200CT99.1% 810.9 / ‑3.9 234P192S (CCG→TCG) tmkthymidylate kinase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (123/108);  total (125/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 245 total aligned reads displayed.

GAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGA  >  NC_000913/1154830‑1155008
                                                                                          |                                                                                        
gAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCg                                                                                        >  2:6519758/1‑93 (MQ=255)
gAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCg                                                                                        >  2:4354260/1‑93 (MQ=255)
 aaTCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGc                                                                                       <  1:4600091/93‑1 (MQ=255)
 aaTCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGc                                                                                       >  2:7338271/1‑93 (MQ=255)
  aTCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCt                                                                                      <  1:4059301/93‑1 (MQ=255)
  aTCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCt                                                                                      <  1:3987129/93‑1 (MQ=255)
   tCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTg                                                                                     >  1:2878189/1‑93 (MQ=255)
   tCTTTCCATTTCTTTAATTGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTg                                                                                     <  2:7169817/93‑1 (MQ=255)
     tttCGATTTCTTTAATCGCCCCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGa                                                                                   <  1:6525481/93‑1 (MQ=255)
     tttCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGa                                                                                   <  2:3626251/93‑1 (MQ=255)
     tttCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGa                                                                                   <  1:3296493/93‑1 (MQ=255)
      ttCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAg                                                                                  <  1:919578/93‑1 (MQ=255)
        cGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGc                                                                                >  1:621898/1‑93 (MQ=255)
        cGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCACTCGCTGGAGGc                                                                                >  2:2541011/1‑93 (MQ=255)
         gATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGcc                                                                               <  2:3792760/93‑1 (MQ=255)
         gATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGcc                                                                               >  2:8155980/1‑93 (MQ=255)
          aTTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCg                                                                              >  1:6300351/1‑93 (MQ=255)
           tttCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGt                                                                             <  2:3051051/93‑1 (MQ=255)
            ttCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTg                                                                            <  1:8580026/93‑1 (MQ=255)
             tCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTga                                                                           <  1:4127978/93‑1 (MQ=255)
               tttAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTgatg                                                                         >  1:5867293/1‑93 (MQ=255)
                 tAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTgatgga                                                                       >  2:4449083/1‑93 (MQ=255)
                 tAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTgatgga                                                                       >  1:748535/1‑93 (MQ=255)
                 tAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTgatgga                                                                       >  1:1838910/1‑93 (MQ=255)
                   aTCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTgatggatg                                                                     >  2:3596518/1‑93 (MQ=255)
                      gCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCaa                                                                  <  2:2649227/93‑1 (MQ=255)
                       cACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAAt                                                                 <  1:2938481/93‑1 (MQ=255)
                          ccGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCg                                                              >  1:4241564/1‑93 (MQ=255)
                           cgcgCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGc                                                             >  1:1031205/1‑93 (MQ=255)
                            gcgcCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCa                                                            >  1:4693922/1‑93 (MQ=255)
                            gcgcCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGAAATCCGCa                                                            >  2:2985430/1‑93 (MQ=255)
                             cgcCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCAc                                                           <  2:8658462/93‑1 (MQ=255)
                               cccGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTa                                                         <  1:6036351/93‑1 (MQ=255)
                                ccGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCTCCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAc                                                        <  2:1201996/93‑1 (MQ=255)
                                ccGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAc                                                        >  1:83162/1‑93 (MQ=255)
                                ccGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAc                                                        >  1:5713255/1‑93 (MQ=255)
                                 cGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAcc                                                       >  1:8157812/1‑93 (MQ=255)
                                 cGCTATCTGGAACTGGCAGCACAAGATAAAAGCATGCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAcc                                                       <  2:8496019/93‑1 (MQ=255)
                                  gCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCg                                                      >  2:8766670/1‑93 (MQ=255)
                                  gCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCg                                                      >  1:2609974/1‑93 (MQ=255)
                                    tATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGta                                                    >  2:790973/1‑92 (MQ=255)
                                    tATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTg                                                    >  1:5380975/1‑93 (MQ=255)
                                      tCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAc                                                  <  2:4205335/93‑1 (MQ=255)
                                      tCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAc                                                  >  1:5140904/1‑93 (MQ=255)
                                      tCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAc                                                  >  1:3828651/1‑93 (MQ=255)
                                         ggAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCa                                               <  1:5266867/93‑1 (MQ=255)
                                          gAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCAc                                              <  1:4814537/93‑1 (MQ=255)
                                           aaCTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACt                                             >  1:2714241/1‑93 (MQ=255)
                                             cTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTgg                                           >  2:137681/1‑93 (MQ=255)
                                             cTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTgg                                           <  1:7082601/93‑1 (MQ=255)
                                              tGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTggg                                          <  2:554581/93‑1 (MQ=255)
                                                 cagcaCAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGa                                       <  1:3559049/93‑1 (MQ=255)
                                                  agcaCAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGGACTACCGTGACCCACTGGGTGaa                                      >  2:4766755/1‑93 (MQ=255)
                                                  agcaCAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGaa                                      <  2:7136135/93‑1 (MQ=255)
                                                  agcaCAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGaa                                      <  1:51865/93‑1 (MQ=255)
                                                   gcaCAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAg                                     <  2:6151975/93‑1 (MQ=255)
                                                    cacaAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATACGCACTACCGTGACCCACTGGGGGAAgg                                    >  2:5461358/1‑93 (MQ=255)
                                                     acaAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGa                                   >  2:1941995/1‑93 (MQ=255)
                                                     acaAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGa                                   >  1:7077852/1‑93 (MQ=255)
                                                     acaAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGa                                   >  1:1436646/1‑93 (MQ=255)
                                                      caAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAg                                  >  1:2517104/1‑93 (MQ=255)
                                                        agagAAAATCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGtt                                <  2:3960892/93‑1 (MQ=255)
                                                        aGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGtt                                >  1:4525081/1‑93 (MQ=255)
                                                         gATAAAAGCATTCATACCATTGATGCCACCCCGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGTAGGAGTTg                               >  2:5237984/1‑93 (MQ=255)
                                                         gATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTg                               >  1:3685289/1‑93 (MQ=255)
                                                           tAAAAGCATTCATACCATTGATGCCTCCCAGTCGCTGGAGACCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGa                             <  2:1446329/93‑1 (MQ=255)
                                                            aaaaGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGAc                            <  2:1793346/93‑1 (MQ=255)
                                                            aaaaGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGAc                            <  1:1878710/93‑1 (MQ=255)
                                                             aaaGCATTCATACCATTGATGCCACCCAGTCGCTGTAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACg                           >  2:6996652/1‑93 (MQ=255)
                                                             aaaGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACg                           >  1:2041638/1‑93 (MQ=255)
                                                             aaaGCATTAATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACg                           >  2:5358973/1‑93 (MQ=255)
                                                                gCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCAt                        >  2:7263597/1‑93 (MQ=255)
                                                                acATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGCGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGGGTTGGACGCAt                        <  2:2093327/92‑1 (MQ=255)
                                                                  aTTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATga                      <  1:1172244/93‑1 (MQ=255)
                                                                   ttCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATgag                     >  2:3165298/1‑93 (MQ=255)
                                                                    tCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATgaga                    <  1:8071456/93‑1 (MQ=255)
                                                                     cATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGAt                   <  1:4185073/93‑1 (MQ=255)
                                                                      aTACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATg                  <  1:6615201/93‑1 (MQ=255)
                                                                       tACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATgg                 <  2:8248300/93‑1 (MQ=255)
                                                                       tACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGAACAACCTTGACCCACTGGGGGAAGGAGTTGGACGCATGGGATgg                 >  2:6385767/1‑93 (MQ=255)
                                                                        aCCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGt                <  2:1513615/93‑1 (MQ=255)
                                                                         ccATTGATGCCACCCAGTCGCTGGAGGCGGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTa               <  1:3388693/93‑1 (MQ=255)
                                                                         ccATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTa               >  2:2325676/1‑93 (MQ=255)
                                                                         ccATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTa               <  2:5048056/93‑1 (MQ=255)
                                                                         ccATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTa               >  2:6701378/1‑93 (MQ=255)
                                                                           aTTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATc             >  2:2340160/1‑93 (MQ=255)
                                                                           aTTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATc             >  1:221452/1‑93 (MQ=255)
                                                                           aTTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGCGCGAAGGAGGTAGAGGCATGAGATGGGATc             >  2:6808308/1‑93 (MQ=255)
                                                                             tgctGCCGCCCAGTCGCTGGAGGTCGTTATGGATGCAATCCGCCCTACCGTGACCCCCTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCa           <  2:5975803/90‑1 (MQ=255)
                                                                             tGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCa           <  2:1702339/93‑1 (MQ=255)
                                                                             tGAAGCCACCCAGACGCTGGAGGCCGTGATGGATGCAATCCGCTCTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCa           <  1:4956261/93‑1 (MQ=255)
                                                                              gATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCAt          <  1:3896977/93‑1 (MQ=255)
                                                                               aTGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATg         <  1:4893434/93‑1 (MQ=255)
                                                                               aTGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATg         <  1:7887992/93‑1 (MQ=255)
                                                                                tGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATgg        <  1:6798276/93‑1 (MQ=255)
                                                                                  ccACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGtt      >  1:5763661/1‑93 (MQ=255)
                                                                                    aCCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTAc    >  1:5603776/1‑93 (MQ=255)
                                                                                     cccAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACg   >  1:580375/1‑93 (MQ=255)
                                                                                      ccAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGa  >  1:8714857/1‑93 (MQ=255)
                                                                                      ccAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGa  >  1:4702540/1‑93 (MQ=255)
                                                                                          |                                                                                        
GAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGA  >  NC_000913/1154830‑1155008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCT  >  NC_000913/1154827‑1155011
                                                                                             |                                                                                           
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGT                                                                                             >  SRR4302541.7356537/1‑94 (MQ=60)
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGT                                                                                             <  SRR4302541.5626654/94‑1 (MQ=60)
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGT                                                                                             >  SRR4302541.4208405/1‑94 (MQ=60)
 AAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTC                                                                                            >  SRR4302541.5651033/1‑94 (MQ=60)
 AAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTC                                                                                            >  SRR4302541.5423259/1‑94 (MQ=60)
  AGAATCTTTCGATTTCTTTAATCTCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCG                                                                                           >  SRR4302541.1528688/1‑94 (MQ=60)
  AGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCG                                                                                           <  SRR4302541.2779901/94‑1 (MQ=60)
    AATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCT                                                                                         <  SRR4302541.4646775/94‑1 (MQ=60)
    AATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCT                                                                                         <  SRR4302541.827317/94‑1 (MQ=60)
    AATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCT                                                                                         >  SRR4302541.5720041/1‑94 (MQ=60)
     ATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTG                                                                                        <  SRR4302541.4099362/94‑1 (MQ=60)
     ATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTG                                                                                        <  SRR4302541.4026627/94‑1 (MQ=60)
     ATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTG                                                                                        >  SRR4302541.2907073/1‑94 (MQ=60)
        TTTCGATTTCTTTAATCGCCCCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAG                                                                                     <  SRR4302541.6592082/94‑1 (MQ=60)
        TTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAG                                                                                     <  SRR4302541.4677790/94‑1 (MQ=60)
        TTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAG                                                                                     <  SRR4302541.3329652/94‑1 (MQ=60)
         TTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGG                                                                                    <  SRR4302541.928185/94‑1 (MQ=60)
          TCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGC                                                                                   >  SRR4302541.627734/1‑94 (MQ=60)
           CGATTTCTTTAATCGCACCCGCTCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCC                                                                                  >  SRR4302541.6671417/1‑94 (MQ=60)
           CGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCC                                                                                  >  SRR4302541.3307183/1‑94 (MQ=60)
           CGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCC                                                                                  >  SRR4302541.5867265/1‑94 (MQ=60)
            GATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCG                                                                                 >  SRR4302541.3751986/1‑94 (MQ=60)
            GATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCG                                                                                 >  SRR4302541.5783528/1‑94 (MQ=60)
            GATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCG                                                                                 >  SRR4302541.5783527/1‑94 (MQ=60)
            GATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCG                                                                                 >  SRR4302541.6364746/1‑94 (MQ=60)
             ATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGT                                                                                >  SRR4302541.4954628/1‑94 (MQ=60)
              TTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTG                                                                               <  SRR4302541.6652362/94‑1 (MQ=60)
               TTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGA                                                                              <  SRR4302541.8672139/94‑1 (MQ=60)
                TCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGAT                                                                             >  SRR4302541.7578620/1‑94 (MQ=60)
                TCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGAT                                                                             <  SRR4302541.4168759/94‑1 (MQ=60)
                TCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGAT                                                                             <  SRR4302541.4168760/94‑1 (MQ=60)
                 CTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATG                                                                            >  SRR4302541.5926981/1‑94 (MQ=60)
                 CTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATG                                                                            <  SRR4302541.475423/94‑1 (MQ=60)
                 CTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATG                                                                            >  SRR4302541.5830555/1‑94 (MQ=60)
                 CTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATG                                                                            >  SRR4302541.8838939/1‑94 (MQ=60)
                   TTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGA                                                                          >  SRR4302541.755617/1‑94 (MQ=60)
                   TTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGA                                                                          >  SRR4302541.1856732/1‑94 (MQ=60)
                    TAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGAT                                                                         <  SRR4302541.8197514/94‑1 (MQ=60)
                     AATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCCCAAGATAAAAGCATTCATACCATTGATGCCCCACAGTCGCTGGCGCCCGTGATGGATG                                                                        >  SRR4302541.2580792/1‑94 (MQ=60)
                       TCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCA                                                                      <  SRR4302541.698381/94‑1 (MQ=60)
                        CGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAA                                                                     >  SRR4302541.4958536/1‑94 (MQ=60)
                         GCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGaact                                                                    >  SRR4302541.115832/1‑90 (MQ=60)
                         GCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAAT                                                                    <  SRR4302541.8080583/94‑1 (MQ=60)
                          CACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATC                                                                   <  SRR4302541.6027379/94‑1 (MQ=60)
                          CACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATC                                                                   <  SRR4302541.941241/94‑1 (MQ=60)
                          CACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATC                                                                   <  SRR4302541.2968034/94‑1 (MQ=60)
                           CCCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCC                                                                  <  SRR4302541.4004079/94‑1 (MQ=60)
                            CCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCG                                                                 >  SRR4302541.4283886/1‑94 (MQ=60)
                             CCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGC                                                                >  SRR4302541.1040727/1‑94 (MQ=60)
                              CGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCA                                                               >  SRR4302541.4741754/1‑94 (MQ=60)
                               GCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCAC                                                              >  SRR4302541.3663588/1‑94 (MQ=60)
                               GCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCAACCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCAC                                                              >  SRR4302541.4043144/1‑94 (MQ=60)
                                CGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACT                                                             >  SRR4302541.4872948/1‑94 (MQ=60)
                                  CCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAC                                                           >  SRR4302541.5771513/1‑94 (MQ=60)
                                  CCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAC                                                           >  SRR4302541.83881/1‑94 (MQ=60)
                                  CCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAC                                                           <  SRR4302541.6098152/94‑1 (MQ=60)
                                  CCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAC                                                           >  SRR4302541.4444001/1‑94 (MQ=60)
                                  CCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTAC                                                           >  SRR4302541.3891401/1‑94 (MQ=60)
                                   CCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACC                                                          >  SRR4302541.8246957/1‑94 (MQ=60)
                                   CCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACC                                                          >  SRR4302541.3825509/1‑94 (MQ=60)
                                    CGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCG                                                         <  SRR4302541.5200354/94‑1 (MQ=60)
                                    CGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCG                                                         >  SRR4302541.2636073/1‑94 (MQ=60)
                                    CGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGACGCTGGAGGCCGTGATGGATGCAATCCGCACTACCG                                                         >  SRR4302541.9218/1‑94 (MQ=60)
                                     GCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGT                                                        >  SRR4302541.6702011/1‑94 (MQ=60)
                                      CTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTG                                                       >  SRR4302541.5436204/1‑94 (MQ=60)
                                      CTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTG                                                       <  SRR4302541.6322643/94‑1 (MQ=60)
                                       TATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGA                                                      >  SRR4302541.3557954/1‑94 (MQ=60)
                                       TATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGA                                                      <  SRR4302541.3139660/94‑1 (MQ=60)
                                        ATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAC                                                     >  SRR4302541.3867221/1‑94 (MQ=60)
                                        ATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAC                                                     >  SRR4302541.5193782/1‑94 (MQ=60)
                                         TCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACC                                                    <  SRR4302541.5420181/94‑1 (MQ=60)
                                          CTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCC                                                   >  SRR4302541.7277544/1‑94 (MQ=60)
                                           TGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCA                                                  <  SRR4302541.5912714/94‑1 (MQ=60)
                                            GGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCAC                                                 <  SRR4302541.5321137/94‑1 (MQ=60)
                                             GAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACT                                                <  SRR4302541.4863626/94‑1 (MQ=60)
                                             GAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACT                                                >  SRR4302541.2741292/1‑94 (MQ=60)
                                             GAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACT                                                >  SRR4302541.2741293/1‑94 (MQ=60)
                                              AACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTG                                               >  SRR4302541.8457739/1‑94 (MQ=60)
                                                CTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGG                                             <  SRR4302541.7157939/94‑1 (MQ=60)
                                                 TGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGT                                            <  SRR4302541.4124713/94‑1 (MQ=60)
                                                 TGACAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGTAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGT                                            <  SRR4302541.4675806/94‑1 (MQ=60)
                                                    CAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAA                                         <  SRR4302541.3595064/94‑1 (MQ=60)
                                                     AGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAG                                        <  SRR4302541.52271/94‑1 (MQ=60)
                                                      GCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGG                                       <  SRR4302541.702689/94‑1 (MQ=60)
                                                       CACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGA                                      >  SRR4302541.7153132/1‑94 (MQ=60)
                                                       CACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGA                                      >  SRR4302541.1450626/1‑94 (MQ=60)
                                                       CACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGA                                      >  SRR4302541.434506/1‑94 (MQ=60)
                                                        ACAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAG                                     >  SRR4302541.2542443/1‑94 (MQ=60)
                                                         CAAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGT                                    <  SRR4302541.6166732/94‑1 (MQ=60)
                                                          AAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTT                                   >  SRR4302541.2172034/1‑94 (MQ=60)
                                                          AAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTT                                   >  SRR4302541.5570741/1‑94 (MQ=60)
                                                          AAGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTT                                   >  SRR4302541.4570750/1‑94 (MQ=60)
                                                           AGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTG                                  >  SRR4302541.8727335/1‑94 (MQ=60)
                                                           AGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTG                                  >  SRR4302541.3722594/1‑94 (MQ=60)
                                                           AGATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGCGAAGGAGTTG                                  >  SRR4302541.2033244/1‑94 (MQ=60)
                                                             ATAAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGA                                >  SRR4302541.4602793/1‑94 (MQ=60)
                                                               AAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACG                              >  SRR4302541.2061342/1‑94 (MQ=60)
                                                               AAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACG                              <  SRR4302541.1896771/94‑1 (MQ=60)
                                                               AAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACG                              <  SRR4302541.5298597/94‑1 (MQ=60)
                                                               AAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACG                              <  SRR4302541.3515404/94‑1 (MQ=60)
                                                               AAAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACG                              >  SRR4302541.1875299/1‑94 (MQ=60)
                                                                AAAGCATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGC                             >  SRR4302541.5060172/1‑94 (MQ=60)
                                                                    CATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGA                         >  SRR4302541.7487680/1‑94 (MQ=60)
                                                                     ATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAG                        >  SRR4302541.1280827/1‑94 (MQ=60)
                                                                     ATTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAG                        <  SRR4302541.1183546/94‑1 (MQ=60)
                                                                      TTCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGA                       <  SRR4302541.2312880/94‑1 (MQ=60)
                                                                       TCATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGAT                      <  SRR4302541.8160061/94‑1 (MQ=60)
                                                                        tatacgcgtggTGCCACCCAGTCGCTGGAGGCGTTGATGGAGGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATG                     <  SRR4302541.8616815/83‑1 (MQ=60)
                                                                        CATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATG                     <  SRR4302541.6496052/94‑1 (MQ=60)
                                                                        CATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATG                     <  SRR4302541.4226596/94‑1 (MQ=60)
                                                                         ATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGG                    <  SRR4302541.4830557/94‑1 (MQ=60)
                                                                         ATACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGG                    <  SRR4302541.6683002/94‑1 (MQ=60)
                                                                           ACCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTA                  >  SRR4302541.5453556/1‑94 (MQ=60)
                                                                            CCATTGATGCCACCCAGTCGCTGGAGGCGGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTAT                 <  SRR4302541.3422488/94‑1 (MQ=60)
                                                                            CCATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTAT                 >  SRR4302541.4273077/1‑94 (MQ=60)
                                                                             CATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATC                >  SRR4302541.223161/1‑94 (MQ=60)
                                                                              ATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCC               >  SRR4302541.2491251/1‑94 (MQ=60)
                                                                              ATTGATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGCTGAAGGAGTTGGACGCATGAGATGGTATCC               >  SRR4302541.4101548/1‑94 (MQ=60)
                                                                                TGAAGCCACCCAGACGCTGGAGGCCGTGATGGATGCAATCCGCTCTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCAT             <  SRR4302541.5006952/94‑1 (MQ=60)
                                                                                 GATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATG            <  SRR4302541.3935996/94‑1 (MQ=60)
                                                                                 GATGCAACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATG            <  SRR4302541.6275814/94‑1 (MQ=60)
                                                                                  agtCCACCCAGTCGCTGGAGTCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGTGTAGTACGCATGAGATGGTATCCATGG           <  SRR4302541.7974715/91‑1 (MQ=60)
                                                                                  ATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGG           <  SRR4302541.4943363/94‑1 (MQ=60)
                                                                                  ATGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGG           <  SRR4302541.7974714/94‑1 (MQ=60)
                                                                                   TGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGT          <  SRR4302541.4000022/94‑1 (MQ=60)
                                                                                   TGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGT          <  SRR4302541.6870980/94‑1 (MQ=60)
                                                                                   TGCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGT          <  SRR4302541.3359123/94‑1 (MQ=60)
                                                                                    GCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTT         <  SRR4302541.7157456/94‑1 (MQ=60)
                                                                                    GCCACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTT         >  SRR4302541.5822316/1‑94 (MQ=60)
                                                                                      CACCCAGTCGCTGGAGTCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTAC       <  SRR4302541.2033039/94‑1 (MQ=60)
                                                                                      CACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTAC       >  SRR4302541.5661137/1‑94 (MQ=60)
                                                                                       ACCCAGTCGCTGGAGGCCGTGATTGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTAGG      >  SRR4302541.2954071/1‑94 (MQ=60)
                                                                                       ACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGGGTTGGACGCATGAGATGGTATCCATGGTTACG      >  SRR4302541.2687362/1‑94 (MQ=60)
                                                                                       ACCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACG      >  SRR4302541.585843/1‑94 (MQ=60)
                                                                                        CCCAGTCGCTGGAGGCCGTGATGGCTGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGCTGGACGCCTGAGCTGCTATCCATGGTTACGA     >  SRR4302541.2810256/1‑94 (MQ=60)
                                                                                        CCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGA     >  SRR4302541.4750479/1‑94 (MQ=60)
                                                                                        CCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGA     >  SRR4302541.4750481/1‑94 (MQ=60)
                                                                                        CCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGA     >  SRR4302541.8808077/1‑94 (MQ=60)
                                                                                        CCCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGA     >  SRR4302541.1570848/1‑94 (MQ=60)
                                                                                         CCAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGAC    <  SRR4302541.8437452/94‑1 (MQ=60)
                                                                                          CAGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACC   >  SRR4302541.3374946/1‑94 (MQ=60)
                                                                                           AGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCT  <  SRR4302541.7175451/94‑1 (MQ=60)
                                                                                           AGTCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCG  >  SRR4302541.712998/1‑94 (MQ=60)
                                                                                             |                                                                                           
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCT  >  NC_000913/1154827‑1155011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑