Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,431	T→C	D235G (GAT→GGT)	sapA ←	SapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,431	0	T	C	98.9%	908.3 / ‑3.1	263	D235G (GAT→GGT)	sapA	SapA
Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (132/128); total (132/131)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 280 total aligned reads displayed.

CCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCC  >  NC_000913/1354341‑1354520

ccGGTCAGGAGTTTCGACAGACGTCCGGTGCCACCGGATCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtcatca                                                                                         >  2:6325890/1‑93 (MQ=255)

   gTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGa                                                                                      <  2:7762222/93‑1 (MQ=255)

    tCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGAc                                                                                     >  1:3109334/1‑93 (MQ=255)

     cAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACg                                                                                    >  2:5457130/1‑93 (MQ=255)

      cggAGTTTCGAACGGCGCCCGGCGCCGCCGGAGCCAAAATCCGCCACTACCTGCGGCATTACCGGGTTACCGCGCCAGAAGTCACCATCACGt                                                                                   <  2:7411665/92‑1 (MQ=255)

      aGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGt                                                                                   <  1:8649053/93‑1 (MQ=255)

      aGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGt                                                                                   >  1:6557392/1‑93 (MQ=255)

       ggAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGtt                                                                                  <  1:8009994/93‑1 (MQ=255)

       ggAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGtt                                                                                  >  2:5133523/1‑93 (MQ=255)

        gAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTg                                                                                 <  1:6334642/93‑1 (MQ=255)

        gAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTg                                                                                 <  1:5621211/93‑1 (MQ=255)

        gAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTg                                                                                 <  1:482813/93‑1 (MQ=255)

         aGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGt                                                                                <  1:7304031/93‑1 (MQ=255)

          gatacGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTa                                                                               <  1:609718/89‑1 (MQ=255)

          gTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTa                                                                               <  2:5763414/93‑1 (MQ=255)

             tCGACAGGCGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGc                                                                            <  1:504472/93‑1 (MQ=255)

               gACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGa                                                                          >  1:3516429/1‑93 (MQ=255)

                      gTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGGCGTTGTAGGCGAATAAAtt                                                                   <  2:2468346/93‑1 (MQ=255)

                      gTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAAtt                                                                   <  2:1953329/93‑1 (MQ=255)

                      gTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAAtt                                                                   >  2:4632975/1‑93 (MQ=255)

                        ccGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGc                                                                 >  2:2875562/1‑93 (MQ=255)

                          ggTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGccc                                                               <  2:3595896/93‑1 (MQ=255)

                            tGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCgg                                                             <  2:1589996/93‑1 (MQ=255)

                            tGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCgg                                                             >  1:2067416/1‑93 (MQ=255)

                              tcgccgGAGCGTAAATCCTCCACTACCTGCGGCATTATCGGTTTACCGCGCAAAAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGgcg                                                           <  2:5500882/92‑1 (MQ=255)

                                gccgGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGcgcg                                                         <  2:5802146/93‑1 (MQ=255)

                                   ggAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTa                                                      >  2:8528299/1‑93 (MQ=255)

                                    gAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTAt                                                     <  2:3904069/93‑1 (MQ=255)

                                     aGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTAtt                                                    >  1:1153651/1‑93 (MQ=255)

                                      gCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTc                                                   <  1:5234750/93‑1 (MQ=255)

                                      gCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTc                                                   <  2:767271/93‑1 (MQ=255)

                                      gCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTc                                                   <  2:5142594/93‑1 (MQ=255)

                                       ccTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTcc                                                  >  1:3933476/1‑93 (MQ=255)

                                        cTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCg                                                 <  1:5605374/93‑1 (MQ=255)

                                        cTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCg                                                 >  1:5355498/1‑93 (MQ=255)

                                        cTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCg                                                 <  2:6761826/93‑1 (MQ=255)

                                        cTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTCCg                                                 >  2:4350445/1‑93 (MQ=255)

                                        cTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTCCg                                                 >  2:40518/1‑93 (MQ=255)

                                         tAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGa                                                >  2:2121895/1‑93 (MQ=255)

                                         tAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGa                                                <  2:1062848/93‑1 (MQ=255)

                                         tAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGa                                                >  2:1819662/1‑93 (MQ=255)

                                           aaTCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACa                                              >  2:4571637/1‑93 (MQ=255)

                                            aTCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACaa                                             >  1:672448/1‑93 (MQ=255)

                                            aTCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACaa                                             >  2:2668676/1‑93 (MQ=255)

                                             tCCACCACTACCTGAGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAAc                                            <  2:8519499/93‑1 (MQ=255)

                                                accacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGa                                         <  1:1122105/93‑1 (MQ=255)

                                                accacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGa                                         <  1:3967248/93‑1 (MQ=255)

                                                accacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGa                                         >  2:8393191/1‑93 (MQ=255)

                                                 ccacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGAt                                        >  2:1696796/1‑93 (MQ=255)

                                                 ccacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGAt                                        <  1:2570504/93‑1 (MQ=255)

                                                  cacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATa                                       >  2:7201357/1‑93 (MQ=255)

                                                  cacTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATa                                       >  2:7554124/1‑93 (MQ=255)

                                                   cctcccTGCGGCATTAACGGTTTACCGCGCCAGAAGTCGCCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATAc                                      <  2:6415340/89‑1 (MQ=255)

                                                      aCCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGTCCTGCGCTGCATTCCTACAACTGATACggt                                   >  2:7367615/1‑92 (MQ=255)

                                                       ccTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGAc                                  <  1:1962862/93‑1 (MQ=255)

                                                        cTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGAcc                                 >  1:453916/1‑93 (MQ=255)

                                                        cTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGAcc                                 <  2:4822936/93‑1 (MQ=255)

                                                         tGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCg                                >  2:4923569/1‑93 (MQ=255)

                                                         tGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCg                                >  2:4883292/1‑93 (MQ=255)

                                                          gCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCgg                               <  1:8115692/93‑1 (MQ=255)

                                                          gCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCgg                               <  2:4189762/93‑1 (MQ=255)

                                                           cGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTCCGACAACTGATCCGGACCggg                              >  2:7969636/1‑92 (MQ=255)

                                                            ggCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTg                             <  1:3309733/93‑1 (MQ=255)

                                                             gCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGc                            <  1:2275729/93‑1 (MQ=255)

                                                             gCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGc                            >  2:7574802/1‑93 (MQ=255)

                                                             gCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGc                            <  1:4009366/93‑1 (MQ=255)

                                                              cATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATCCGGACCGGTGcc                           >  1:2800985/1‑93 (MQ=255)

                                                               aTTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCAACAACTGATACGGACCGGTGCCg                          >  1:7739171/1‑93 (MQ=255)

                                                                ttAACGGTTTACCGCGTCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTCAGACAACCGATCCGGCCCGGTGCCgc                         >  2:5931696/1‑92 (MQ=255)

                                                                ttAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCgc                         >  2:6522441/1‑92 (MQ=255)

                                                                ttAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGa                         <  2:6841599/93‑1 (MQ=255)

                                                                ttAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTCCGACAACTGATACGGACCGATGCCgc                         >  1:7260397/1‑92 (MQ=255)

                                                                   aCGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCg                      >  1:6146016/1‑93 (MQ=255)

                                                                    cGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCgg                     >  2:831113/1‑93 (MQ=255)

                                                                     ggTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGt                    <  1:8584042/93‑1 (MQ=255)

                                                                     ggTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGt                    <  2:6232156/93‑1 (MQ=255)

                                                                      gTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGtt                   >  2:3679290/1‑93 (MQ=255)

                                                                      gTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGtt                   <  1:1305583/93‑1 (MQ=255)

                                                                      gTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGtt                   >  1:1692104/1‑93 (MQ=255)

                                                                         tACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGAc                <  2:1459564/93‑1 (MQ=255)

                                                                          aCCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACg               <  1:6247275/93‑1 (MQ=255)

                                                                           acgcgCCAGTAGTCATCATGTTCTTGTAGGCTAATGAAGTGCACGGCTTGGTATTCCGACAACTGACACTGACCGGTGCCGACCGGTTGACgg              <  1:6007654/92‑1 (MQ=255)

                                                                            cgcgCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGt             <  1:142743/93‑1 (MQ=255)

                                                                            cgcgCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGt             <  1:8756117/93‑1 (MQ=255)

                                                                               gcCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGa          >  2:6100450/1‑93 (MQ=255)

                                                                                ccAGTAGTCGCCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAg         <  2:123705/93‑1 (MQ=255)

                                                                                 cAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGt        <  1:7449498/93‑1 (MQ=255)

                                                                                 cAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGt        <  2:1884409/93‑1 (MQ=255)

                                                                                 cAGAAGTCACCATGACGGTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGt        <  2:7123981/93‑1 (MQ=255)

                                                                                  aGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGtt       <  1:3247369/93‑1 (MQ=255)

                                                                                  aGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGtt       <  2:843194/93‑1 (MQ=255)

                                                                                   gAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTg      <  1:8355466/93‑1 (MQ=255)

                                                                                   gAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTg      >  2:3300669/1‑93 (MQ=255)

                                                                                    aaGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGc     <  1:6843489/93‑1 (MQ=255)

                                                                                      gTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTc   >  1:4257506/1‑93 (MQ=255)

                                                                                      gTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTc   <  1:6654404/93‑1 (MQ=255)

                                                                                      gTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTc   >  2:4246373/1‑93 (MQ=255)

                                                                                       tcaccaTGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTcc  <  1:999488/93‑1 (MQ=255)

                                                                                       tcaccaTGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTcc  <  2:7262719/93‑1 (MQ=255)

                                                                                       tcaccaTGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTcc  <  2:5348107/93‑1 (MQ=255)

CCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCC  >  NC_000913/1354341‑1354520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGC  >  NC_000913/1354339‑1354524

CCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACC                                                                                              >  SRR4302541.7067720/1‑94 (MQ=60)

 CCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCA                                                                                             <  SRR4302541.5926648/94‑1 (MQ=60)

 CCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCA                                                                                             <  SRR4302541.5926649/94‑1 (MQ=60)

  CCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCAT                                                                                            >  SRR4302541.5085570/1‑94 (MQ=60)

     GTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGAC                                                                                         >  SRR4302541.3140809/1‑94 (MQ=60)

      TCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACG                                                                                        >  SRR4302541.8690838/1‑94 (MQ=60)

       CAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGT                                                                                       >  SRR4302541.6624432/1‑94 (MQ=60)

       CAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGT                                                                                       >  SRR4302541.61331/1‑94 (MQ=60)

        AGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTT                                                                                      <  SRR4302541.8741666/94‑1 (MQ=60)

        AGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTT                                                                                      >  SRR4302541.2225285/1‑94 (MQ=60)

         GGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTG                                                                                     <  SRR4302541.8098087/94‑1 (MQ=60)

         GGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTG                                                                                     <  SRR4302541.732985/94‑1 (MQ=60)

          GAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGT                                                                                    <  SRR4302541.5678730/94‑1 (MQ=60)

          GAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGT                                                                                    <  SRR4302541.487385/94‑1 (MQ=60)

          GAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGT                                                                                    <  SRR4302541.6399401/94‑1 (MQ=60)

           AGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTA                                                                                   <  SRR4302541.7382001/94‑1 (MQ=60)

           AGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTA                                                                                   <  SRR4302541.3312730/94‑1 (MQ=60)

            gataCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAG                                                                                  <  SRR4302541.615450/90‑1 (MQ=60)

            GTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAG                                                                                  <  SRR4302541.8863700/94‑1 (MQ=60)

            GTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAG                                                                                  <  SRR4302541.6304731/94‑1 (MQ=60)

            GTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAG                                                                                  <  SRR4302541.8427866/94‑1 (MQ=60)

               TCGACAGGCGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCG                                                                               <  SRR4302541.509210/94‑1 (MQ=60)

                CGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGA                                                                              >  SRR4302541.3551800/1‑94 (MQ=60)

                  ACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAAT                                                                            >  SRR4302541.1929096/1‑94 (MQ=60)

                   CAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATA                                                                           <  SRR4302541.7194352/94‑1 (MQ=60)

                    AGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAA                                                                          >  SRR4302541.2731430/1‑94 (MQ=60)

                    AGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAA                                                                          >  SRR4302541.3499727/1‑94 (MQ=60)

                    AGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAA                                                                          >  SRR4302541.6276305/1‑94 (MQ=60)

                    AGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAA                                                                          <  SRR4302541.6190551/94‑1 (MQ=60)

                      ACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAAT                                                                        <  SRR4302541.8818641/94‑1 (MQ=60)

                      ACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAAT                                                                        >  SRR4302541.3554628/1‑94 (MQ=60)

                       CGTCCGGTGCCGCCGGAGCCTAAATCCACGACTGCCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATT                                                                       <  SRR4302541.3055034/94‑1 (MQ=60)

                        GTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTG                                                                      <  SRR4302541.5064949/94‑1 (MQ=60)

                        GTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTG                                                                      <  SRR4302541.3370094/94‑1 (MQ=60)

                        GTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTG                                                                      >  SRR4302541.5784705/1‑94 (MQ=60)

                        GTCCGGTGCCGCCGGAGCATAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTG                                                                      <  SRR4302541.5186806/94‑1 (MQ=60)

                         TCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGC                                                                     <  SRR4302541.7127335/94‑1 (MQ=60)

                         TCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGC                                                                     >  SRR4302541.8184349/1‑94 (MQ=60)

                          CCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCC                                                                    <  SRR4302541.3575397/94‑1 (MQ=60)

                            GGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCG                                                                  >  SRR4302541.4161446/1‑94 (MQ=60)

                             GTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGG                                                                 >  SRR4302541.4722316/1‑94 (MQ=60)

                             GTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGG                                                                 >  SRR4302541.2087391/1‑94 (MQ=60)

                              TGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGC                                                                <  SRR4302541.4305873/94‑1 (MQ=60)

                               GCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCG                                                               <  SRR4302541.7348781/94‑1 (MQ=60)

                                  GCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCTCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGG                                                            <  SRR4302541.3996097/94‑1 (MQ=60)

                                  GCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGG                                                            <  SRR4302541.135666/94‑1 (MQ=60)

                                    CGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTA                                                          >  SRR4302541.6828941/1‑94 (MQ=60)

                                      GAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATT                                                        <  SRR4302541.1434479/94‑1 (MQ=60)

                                      GAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATT                                                        >  SRR4302541.1164682/1‑94 (MQ=60)

                                      GAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATT                                                        <  SRR4302541.7436598/94‑1 (MQ=60)

                                       AGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTC                                                       >  SRR4302541.3272087/1‑94 (MQ=60)

                                       AGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTC                                                       >  SRR4302541.5669646/1‑94 (MQ=60)

                                        GCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCC                                                      <  SRR4302541.8683743/94‑1 (MQ=60)

                                        GCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCC                                                      >  SRR4302541.3972652/1‑94 (MQ=60)

                                        GCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCC                                                      <  SRR4302541.7598312/94‑1 (MQ=60)

                                        GCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCC                                                      <  SRR4302541.5288683/94‑1 (MQ=60)

                                         CCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCG                                                     <  SRR4302541.6002577/94‑1 (MQ=60)

                                         CCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCG                                                     <  SRR4302541.3326152/94‑1 (MQ=60)

                                         CCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCG                                                     >  SRR4302541.5410520/1‑94 (MQ=60)

                                          CTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGA                                                    >  SRR4302541.6476178/1‑94 (MQ=60)

                                          CTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGA                                                    >  SRR4302541.1287790/1‑94 (MQ=60)

                                          CTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGA                                                    >  SRR4302541.8767415/1‑94 (MQ=60)

                                          CTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGA                                                    <  SRR4302541.5662747/94‑1 (MQ=60)

                                            AAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACA                                                  <  SRR4302541.3977775/94‑1 (MQ=60)

                                             AATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAA                                                 <  SRR4302541.1139553/94‑1 (MQ=60)

                                             AATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAA                                                 >  SRR4302541.678800/1‑94 (MQ=60)

                                               CCCACCACTACCTGCGGCATTAACGGTCAGACGCGCCAGAACTCCCCATGACGTTGTAGGCAAATAAATTGCCCGGCGCGGTATTCCGACAACG                                               <  SRR4302541.4467805/94‑1 (MQ=60)

                                                 CACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGA                                             >  SRR4302541.5324610/1‑94 (MQ=60)

                                                  ACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGAT                                            <  SRR4302541.4006634/94‑1 (MQ=60)

                                                  ACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGAT                                            <  SRR4302541.1132668/94‑1 (MQ=60)

                                                   CCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATA                                           <  SRR4302541.6810169/94‑1 (MQ=60)

                                                   CCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATA                                           <  SRR4302541.2596275/94‑1 (MQ=60)

                                                     ACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACG                                         <  SRR4302541.801815/94‑1 (MQ=60)

                                                      CTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGG                                        <  SRR4302541.2239929/94‑1 (MQ=60)

                                                      CTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGG                                        <  SRR4302541.3060646/94‑1 (MQ=60)

                                                        ACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGAC                                      <  SRR4302541.6738071/94‑1 (MQ=60)

                                                        ACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACCGATACGGAC                                      >  SRR4302541.2050462/1‑94 (MQ=60)

                                                         CCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACC                                     <  SRR4302541.252317/94‑1 (MQ=60)

                                                         CCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACC                                     <  SRR4302541.2544782/94‑1 (MQ=60)

                                                         CCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACC                                     <  SRR4302541.1981788/94‑1 (MQ=60)

                                                         CCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACC                                     >  SRR4302541.458244/1‑94 (MQ=60)

                                                            GCGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGT                                  <  SRR4302541.8204598/94‑1 (MQ=60)

                                                             CGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTG                                 >  SRR4302541.193665/1‑94 (MQ=60)

                                                             CGGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTG                                 >  SRR4302541.5485819/1‑94 (MQ=60)

                                                              GGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGC                                <  SRR4302541.3342967/94‑1 (MQ=60)

                                                              GGCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGC                                >  SRR4302541.1703657/1‑94 (MQ=60)

                                                               GCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATCCGGACCGGTGCC                               >  SRR4302541.2829302/1‑94 (MQ=60)

                                                               GCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCC                               <  SRR4302541.4049031/94‑1 (MQ=60)

                                                               GCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCC                               <  SRR4302541.1536387/94‑1 (MQ=60)

                                                               GCATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCC                               <  SRR4302541.2297734/94‑1 (MQ=60)

                                                                CATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCAACAACTGATACGGACCGGTGCCG                              >  SRR4302541.7821511/1‑94 (MQ=60)

                                                                CATTAACGCTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCAACAACTGATACGGACCGGTGCCG                              >  SRR4302541.7821510/1‑94 (MQ=60)

                                                                 ATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGC                             >  SRR4302541.1317647/1‑94 (MQ=60)

                                                                 ATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGA                             >  SRR4302541.6331301/1‑94 (MQ=60)

                                                                 ATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGA                             >  SRR4302541.4027704/1‑94 (MQ=60)

                                                                 ATTAACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGCATTCCGACAACTGATACGGACCGATGCCGC                             >  SRR4302541.7337850/1‑94 (MQ=60)

                                                                    AACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCG                          <  SRR4302541.968492/94‑1 (MQ=60)

                                                                    AACGGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCG                          >  SRR4302541.6208807/1‑94 (MQ=60)

                                                                       GGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTT                       <  SRR4302541.8676190/94‑1 (MQ=60)

                                                                       GGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTT                       >  SRR4302541.1708715/1‑94 (MQ=60)

                                                                       GGTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTT                       >  SRR4302541.4740440/1‑94 (MQ=60)

                                                                        GTTTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTG                      <  SRR4302541.1318446/94‑1 (MQ=60)

                                                                          TTACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGAC                    >  SRR4302541.6382092/1‑94 (MQ=60)

                                                                            ACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGG                  >  SRR4302541.8203843/1‑94 (MQ=60)

                                                                            ACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGG                  <  SRR4302541.6311099/94‑1 (MQ=60)

                                                                            ACCGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGG                  >  SRR4302541.5677628/1‑94 (MQ=60)

                                                                              CGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTC                <  SRR4302541.143840/94‑1 (MQ=60)

                                                                              CGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTC                >  SRR4302541.4492625/1‑94 (MQ=60)

                                                                              CGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTC                <  SRR4302541.8849781/94‑1 (MQ=60)

                                                                              CGCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTC                <  SRR4302541.6721966/94‑1 (MQ=60)

                                                                               GCGCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCG               <  SRR4302541.2613511/94‑1 (MQ=60)

                                                                                 GCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAG             <  SRR4302541.6173358/94‑1 (MQ=60)

                                                                                 GCCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAG             <  SRR4302541.8457752/94‑1 (MQ=60)

                                                                                  CCAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGT            >  SRR4302541.8344604/1‑94 (MQ=60)

                                                                                   CAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGCCGGTCGAGTT           >  SRR4302541.6514505/1‑94 (MQ=60)

                                                                                   CAGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTT           <  SRR4302541.7529074/94‑1 (MQ=60)

                                                                                   CAGAAGTCACCATGACATTATAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTT           <  SRR4302541.7875284/94‑1 (MQ=60)

                                                                                    AGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTG          <  SRR4302541.3280153/94‑1 (MQ=60)

                                                                                    AGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTG          >  SRR4302541.4149813/1‑94 (MQ=60)

                                                                                    AGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTG          >  SRR4302541.5306034/1‑94 (MQ=60)

                                                                                    AGAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTG          <  SRR4302541.542358/94‑1 (MQ=60)

                                                                                     GAAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGC         <  SRR4302541.8446125/94‑1 (MQ=60)

                                                                                      AAGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCT        <  SRR4302541.6916664/94‑1 (MQ=60)

                                                                                       AGTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTC       >  SRR4302541.4300061/1‑94 (MQ=60)

                                                                                        GTCAGCATGACGTTGTACGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCC      <  SRR4302541.6722675/94‑1 (MQ=60)

                                                                                        GTCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCC      <  SRR4302541.6722674/94‑1 (MQ=60)

                                                                                         TCACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCT     <  SRR4302541.1008790/94‑1 (MQ=60)

                                                                                          CACCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTG    >  SRR4302541.5414096/1‑94 (MQ=60)

                                                                                            CCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGC  <  SRR4302541.4716409/94‑1 (MQ=60)

                                                                                            CCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGC  >  SRR4302541.6933175/1‑94 (MQ=60)

CCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCGGTATTCCGACAACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGC  >  NC_000913/1354339‑1354524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`