Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,415,996 (T)6→7 coding (37/171 nt) ydaE ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,415,9901.T99.6% 966.2 / ‑4.2 269coding (43/171 nt)ydaEhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (145/123);  total (146/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 309 total aligned reads displayed.

AGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAG  >  NC_000913/1415898‑1416077
                                                                                             |                                                                                       
 gCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑t                                                                                        <  1:3651800/93‑1 (MQ=255)
  cTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tt                                                                                       <  1:8331428/93‑1 (MQ=255)
   ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttt                                                                                      >  2:717701/1‑93 (MQ=255)
   ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttt                                                                                      >  2:7057284/1‑93 (MQ=255)
     cACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttt                                                                                    <  1:6200428/93‑1 (MQ=255)
      aCTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttt                                                                                   >  2:4057098/1‑93 (MQ=255)
      aCTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttt                                                                                   <  1:8459927/93‑1 (MQ=255)
          cAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttttgca                                                                               >  2:1618837/1‑89 (MQ=255)
            atatTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCaaa                                                                              <  2:7372107/93‑1 (MQ=255)
            atatTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCaaa                                                                              <  1:3863044/93‑1 (MQ=255)
             tatTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAg                                                                             <  2:6465830/93‑1 (MQ=255)
             tatTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAg                                                                             >  2:6360333/1‑93 (MQ=255)
              atTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAgg                                                                            <  1:6671251/93‑1 (MQ=255)
               ttttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggt                                                                           >  2:378077/1‑93 (MQ=255)
                tttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtg                                                                          >  2:7427025/1‑93 (MQ=255)
                tttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtg                                                                          <  1:6750543/93‑1 (MQ=255)
                tttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtg                                                                          <  2:1563094/93‑1 (MQ=255)
                 ttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtgg                                                                         <  2:3843948/93‑1 (MQ=255)
                 ttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtgg                                                                         >  2:7336759/1‑93 (MQ=255)
                  tctcGGTTCGTCTTTTGATAAAATACCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtcgt                                                                        >  2:6088050/1‑93 (MQ=255)
                    tcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTaa                                                                      >  1:1198810/1‑93 (MQ=255)
                    tcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTaa                                                                      >  1:1032457/1‑93 (MQ=255)
                    tcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTaa                                                                      >  1:2367391/1‑93 (MQ=255)
                     cGGTTTGTTTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAg                                                                     <  2:6734496/93‑1 (MQ=255)
                     cGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAg                                                                     >  2:3618714/1‑93 (MQ=255)
                     cGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAg                                                                     <  2:6526154/93‑1 (MQ=255)
                      ggTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTCCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGc                                                                    >  1:7764117/1‑93 (MQ=255)
                       gTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGca                                                                   >  2:3011761/1‑93 (MQ=255)
                       gTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGca                                                                   >  1:6321845/1‑93 (MQ=255)
                        ttCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcac                                                                  >  1:8088484/1‑93 (MQ=255)
                         tCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcaca                                                                 >  2:8007572/1‑93 (MQ=255)
                         tCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcaca                                                                 <  1:1986612/93‑1 (MQ=255)
                          cGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACat                                                                <  2:2665427/93‑1 (MQ=255)
                          cGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACat                                                                >  2:6821370/1‑93 (MQ=255)
                          cGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACat                                                                >  2:5198515/1‑93 (MQ=255)
                           gTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatt                                                               >  2:8459795/1‑93 (MQ=255)
                            tCTTTTGATAAAATCCCTTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattt                                                              >  1:8160549/1‑93 (MQ=255)
                            tCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattt                                                              >  2:5756060/1‑93 (MQ=255)
                            tCTTTTGATAAAATCACGTGCATGAAGGGAAGCATTCTTTCAATAGCTTTGATTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattt                                                              >  1:4388616/1‑93 (MQ=255)
                               tttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattttat                                                           >  1:5694827/1‑93 (MQ=255)
                                ttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattttatt                                                          >  2:2118237/1‑93 (MQ=255)
                                ttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattttatt                                                          >  2:8154341/1‑93 (MQ=255)
                                  gATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACattttatttt                                                        <  2:4323173/93‑1 (MQ=255)
                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTc                                                       <  1:7221855/93‑1 (MQ=255)
                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTc                                                       <  2:3280060/93‑1 (MQ=255)
                                    tAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCt                                                      <  2:8501983/93‑1 (MQ=255)
                                     aaaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCtt                                                     <  1:1231767/93‑1 (MQ=255)
                                      aaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTa                                                    <  2:5110018/93‑1 (MQ=255)
                                      aaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTa                                                    >  2:8605604/1‑93 (MQ=255)
                                       aaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAg                                                   <  2:7122436/93‑1 (MQ=255)
                                        aTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGt                                                  >  2:5297510/1‑93 (MQ=255)
                                         tCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtc                                                 <  2:5927033/93‑1 (MQ=255)
                                          cccGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtca                                                <  2:1028504/93‑1 (MQ=255)
                                           ccGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcat                                               >  1:1482956/1‑93 (MQ=255)
                                            cGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcatc                                              <  2:7679836/93‑1 (MQ=255)
                                              tGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCAc                                            >  1:7367887/1‑93 (MQ=255)
                                                cATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                          <  1:4024934/93‑1 (MQ=255)
                                                cATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                          <  1:1720863/93‑1 (MQ=255)
                                                 aTGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAt                                         >  1:4880210/1‑93 (MQ=255)
                                                 aTGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAt                                         <  2:4907412/93‑1 (MQ=255)
                                                  tgaagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATg                                        >  1:8766492/1‑93 (MQ=255)
                                                    aagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGAc                                      >  1:3128581/1‑93 (MQ=255)
                                                    aagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGAc                                      >  2:5480996/1‑93 (MQ=255)
                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                    >  1:7587019/1‑93 (MQ=255)
                                                      atgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                    <  1:4561966/92‑1 (MQ=255)
                                                           gCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCt                               >  1:2554378/1‑93 (MQ=255)
                                                           gCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCt                               <  2:3495347/93‑1 (MQ=255)
                                                            cATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCtt                              <  2:8553946/93‑1 (MQ=255)
                                                            cATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCtt                              <  1:2027497/93‑1 (MQ=255)
                                                             aTTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCttt                             >  2:217677/1‑93 (MQ=255)
                                                               tCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTAc                           <  2:1382821/93‑1 (MQ=255)
                                                                cTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACa                          >  1:609671/1‑93 (MQ=255)
                                                                 tttCGATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAg                         >  1:7857892/1‑93 (MQ=255)
                                                                  ttCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAgg                        <  1:2154431/93‑1 (MQ=255)
                                                                     aaTAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaa                     >  1:8006883/1‑93 (MQ=255)
                                                                      aTAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaaa                    >  1:5789083/1‑93 (MQ=255)
                                                                       tAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAAc                   <  1:4227040/93‑1 (MQ=255)
                                                                        aGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAAcc                  <  1:889893/93‑1 (MQ=255)
                                                                        aGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAAcc                  <  1:8584839/93‑1 (MQ=255)
                                                                         gctttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCa                 <  1:4813157/93‑1 (MQ=255)
                                                                          ctttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAt                >  1:5489801/1‑93 (MQ=255)
                                                                          ctttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAt                <  2:1905705/93‑1 (MQ=255)
                                                                          ctttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAt                <  2:395518/93‑1 (MQ=255)
                                                                           tttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATc               <  2:1249293/93‑1 (MQ=255)
                                                                             tgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCAc             <  2:6936788/93‑1 (MQ=255)
                                                                              gctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACg            >  2:6248543/1‑93 (MQ=255)
                                                                              gctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACg            >  2:5878204/1‑93 (MQ=255)
                                                                              gctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACg            >  1:4152331/1‑93 (MQ=255)
                                                                                tttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAc          >  1:5181895/1‑93 (MQ=255)
                                                                                tttCTTCAACGTCTTTTTTTGCAAAGGGGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAc          >  2:7515834/1‑93 (MQ=255)
                                                                                  tcttcAACGTCTTTTTTTGCAAAGGTGGTAAGCGCATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCg        <  1:6704604/93‑1 (MQ=255)
                                                                                  tcttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCg        >  1:7037732/1‑93 (MQ=255)
                                                                                  tcttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCg        >  1:2819548/1‑93 (MQ=255)
                                                                                  tcttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCg        <  2:2291241/93‑1 (MQ=255)
                                                                                    ttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAg      >  2:7862547/1‑93 (MQ=255)
                                                                                    ttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAg      <  2:3082000/93‑1 (MQ=255)
                                                                                     tcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCTTTTACAGGTAAACCATCACGACCGAgg     <  2:4584544/93‑1 (MQ=255)
                                                                                     tcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg     >  2:2889269/1‑93 (MQ=255)
                                                                                       aaCGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGaa   >  2:2436848/1‑93 (MQ=255)
                                                                                       aaCGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGaa   >  1:299518/1‑93 (MQ=255)
                                                                                             |                                                                                       
AGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAG  >  NC_000913/1415898‑1416077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATG  >  NC_000913/1415903‑1416089
                                                                                              |                                                                                             
CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTT                                                                                               <  SRR4302541.6263785/94‑1 (MQ=60)
CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTT                                                                                               >  SRR4302541.2018346/1‑94 (MQ=60)
 ACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTT                                                                                              <  SRR4302541.8551192/94‑1 (MQ=60)
 ACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTT                                                                                              >  SRR4302541.1585707/1‑94 (MQ=60)
   TGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTtgc                                                                                            >  SRR4302541.2758022/1‑91 (MQ=60)
     CAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAA                                                                                           >  SRR4302541.3557606/1‑94 (MQ=60)
       ATATTTTCTCGGTTCTGCTTTTGATAAAATCCTGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAG                                                                                         <  SRR4302541.2182167/94‑1 (MQ=60)
       ATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAG                                                                                         <  SRR4302541.3901903/94‑1 (MQ=60)
       ATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAG                                                                                         >  SRR4302541.8495892/1‑94 (MQ=60)
        TATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGG                                                                                        >  SRR4302541.6087118/1‑94 (MQ=60)
         ATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGT                                                                                       <  SRR4302541.8918430/94‑1 (MQ=60)
         ATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGT                                                                                       <  SRR4302541.6739881/94‑1 (MQ=60)
           TTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGG                                                                                     <  SRR4302541.3137749/94‑1 (MQ=60)
           TTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGG                                                                                     <  SRR4302541.6283344/94‑1 (MQ=60)
           TTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGG                                                                                     <  SRR4302541.6822704/94‑1 (MQ=60)
           TTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGG                                                                                     <  SRR4302541.6822705/94‑1 (MQ=60)
            TTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGT                                                                                    >  SRR4302541.44202/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  <  SRR4302541.6767563/94‑1 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302541.8574359/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302541.1210438/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302541.52490/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302541.2390489/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302541.1041984/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302541.1627016/1‑94 (MQ=60)
              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGGGGTAA                                                                                  >  SRR4302541.3738510/1‑94 (MQ=60)
               TCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAG                                                                                 <  SRR4302541.7402782/94‑1 (MQ=60)
               TCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAG                                                                                 <  SRR4302541.2963467/94‑1 (MQ=60)
                CGGTTCGTCTTTTGATCAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGC                                                                                >  SRR4302541.3666138/1‑94 (MQ=60)
                CGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGC                                                                                >  SRR4302541.44196/1‑94 (MQ=60)
                CGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTCCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGC                                                                                >  SRR4302541.7846716/1‑94 (MQ=60)
                 GGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCA                                                                               <  SRR4302541.810015/94‑1 (MQ=60)
                 GGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCA                                                                               >  SRR4302541.8276611/1‑94 (MQ=60)
                 GGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCA                                                                               >  SRR4302541.6386479/1‑94 (MQ=60)
                  GTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCACAGGTGGTAAGAAC                                                                              >  SRR4302541.6405869/1‑94 (MQ=60)
                  GTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCAC                                                                              >  SRR4302541.5282144/1‑94 (MQ=60)
                  GTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCAC                                                                              >  SRR4302541.8177162/1‑94 (MQ=60)
                   TTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACA                                                                             >  SRR4302541.992338/1‑94 (MQ=60)
                    TCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACAT                                                                            <  SRR4302541.2005820/94‑1 (MQ=60)
                     CGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATT                                                                           <  SRR4302541.8668816/94‑1 (MQ=60)
                     CGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATT                                                                           >  SRR4302541.7296817/1‑94 (MQ=60)
                     CGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATT                                                                           >  SRR4302541.837330/1‑94 (MQ=60)
                      GTCTTTTGATAAAATCCCTTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302541.8249707/1‑94 (MQ=60)
                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302541.4199647/1‑94 (MQ=60)
                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302541.8714044/1‑94 (MQ=60)
                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302541.1197612/1‑94 (MQ=60)
                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          <  SRR4302541.3911363/94‑1 (MQ=60)
                      GTCTTTTGATAAAATCACGTGCATGAAGGGAAGCATTCTTTCAATAGCTTTGATTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302541.4432514/1‑94 (MQ=60)
                       TCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTT                                                                         >  SRR4302541.6759387/1‑94 (MQ=60)
                        CTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTA                                                                        >  SRR4302541.123071/1‑94 (MQ=60)
                         TTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTAT                                                                       >  SRR4302541.5752902/1‑94 (MQ=60)
                          TTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATT                                                                      <  SRR4302541.1165513/94‑1 (MQ=60)
                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    >  SRR4302541.6816509/1‑94 (MQ=60)
                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    >  SRR4302541.4481192/1‑94 (MQ=60)
                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    >  SRR4302541.4481193/1‑94 (MQ=60)
                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    <  SRR4302541.4214333/94‑1 (MQ=60)
                              ATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCT                                                                  <  SRR4302541.7298901/94‑1 (MQ=60)
                              ATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCT                                                                  <  SRR4302541.7298902/94‑1 (MQ=60)
                                AAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTA                                                                <  SRR4302541.1243882/94‑1 (MQ=60)
                                AAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTA                                                                <  SRR4302541.1243881/94‑1 (MQ=60)
                                AAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTA                                                                <  SRR4302541.8222233/94‑1 (MQ=60)
                                 AAATCCCGTGCATGAGGTCAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAG                                                               <  SRR4302541.7476137/94‑1 (MQ=60)
                                 AAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAG                                                               <  SRR4302541.6178532/94‑1 (MQ=60)
                                  AATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGT                                                              <  SRR4302541.739159/94‑1 (MQ=60)
                                  AATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGT                                                              <  SRR4302541.2743258/94‑1 (MQ=60)
                                   ATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTC                                                             >  SRR4302541.3162045/1‑94 (MQ=60)
                                   ATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTC                                                             <  SRR4302541.2141704/94‑1 (MQ=60)
                                     CCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCAT                                                           >  SRR4302541.1497534/1‑94 (MQ=60)
                                     CCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCAT                                                           >  SRR4302541.1497535/1‑94 (MQ=60)
                                      CCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATC                                                          <  SRR4302541.778716/94‑1 (MQ=60)
                                      CCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATC                                                          >  SRR4302541.4997588/1‑94 (MQ=60)
                                       CGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCA                                                         <  SRR4302541.4911133/94‑1 (MQ=60)
                                        GTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCAC                                                        >  SRR4302541.7446638/1‑94 (MQ=60)
                                         TGCATGAAGTGAAGCATTCTTTCAATAGCTTAGCTTTCTTCATCGTCTTTTTTTGCAAAGGCGGTAAGCACATTTTATTTTCTTAGTCATCACC                                                       >  SRR4302541.5882828/1‑94 (MQ=60)
                                           CATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAT                                                     <  SRR4302541.1737686/94‑1 (MQ=60)
                                           CATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAT                                                     <  SRR4302541.4064702/94‑1 (MQ=60)
                                           CATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAT                                                     >  SRR4302541.4930014/1‑94 (MQ=60)
                                           CATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTATCTTTGTCATCACCAT                                                     >  SRR4302541.7931629/1‑94 (MQ=60)
                                            ATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATG                                                    >  SRR4302541.8860231/1‑94 (MQ=60)
                                            ATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATG                                                    <  SRR4302541.1265352/94‑1 (MQ=60)
                                             TGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGA                                                   <  SRR4302541.7989375/94‑1 (MQ=60)
                                              GAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGAC                                                  >  SRR4302541.3160211/1‑94 (MQ=60)
                                                AGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTC                                                >  SRR4302541.7668081/1‑94 (MQ=60)
                                                AGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTC                                                >  SRR4302541.2917140/1‑94 (MQ=60)
                                                AGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTC                                                >  SRR4302541.7668082/1‑94 (MQ=60)
                                                 GTGAAGCATTCTTTCGATAGCTTTGCTTTCTTCGACGTCTTTTTTTGCAAAGGTGGTAAGCACTTTTTATTTTCTTAGTCATCACCATGACTCC                                               <  SRR4302541.7903712/94‑1 (MQ=60)
                                                 GTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCC                                               <  SRR4302541.4867001/94‑1 (MQ=60)
                                                 GTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCC                                               >  SRR4302541.2470683/1‑94 (MQ=60)
                                                 GTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCC                                               <  SRR4302541.2056755/94‑1 (MQ=60)
                                                 ATGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCC                                               <  SRR4302541.4608148/94‑1 (MQ=60)
                                                  TGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCG                                              <  SRR4302541.1949119/94‑1 (MQ=60)
                                                  TGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCG                                              <  SRR4302541.2556077/94‑1 (MQ=60)
                                                     AGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCT                                           >  SRR4302541.56650/1‑94 (MQ=60)
                                                     AGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCT                                           <  SRR4302541.8165718/94‑1 (MQ=60)
                                                     AGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCT                                           >  SRR4302541.2579986/1‑94 (MQ=60)
                                                       CATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTT                                         <  SRR4302541.2047089/94‑1 (MQ=60)
                                                          TCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACA                                      >  SRR4302541.615403/1‑94 (MQ=60)
                                                           CTTTCGATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAG                                     >  SRR4302541.7944181/1‑94 (MQ=60)
                                                             TTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGT                                   <  SRR4302541.2175260/94‑1 (MQ=60)
                                                               CAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAA                                 >  SRR4302541.8094959/1‑94 (MQ=60)
                                                                AATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAA                                >  SRR4302541.4149886/1‑94 (MQ=60)
                                                                AATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAA                                >  SRR4302541.4149885/1‑94 (MQ=60)
                                                                AATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAA                                >  SRR4302541.5847910/1‑94 (MQ=60)
                                                                AATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAA                                >  SRR4302541.3127635/1‑94 (MQ=60)
                                                                AATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAA                                >  SRR4302541.3989138/1‑94 (MQ=60)
                                                                  TAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACC                              <  SRR4302541.4269141/94‑1 (MQ=60)
                                                                  TAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACC                              <  SRR4302541.961530/94‑1 (MQ=60)
                                                                   AGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCA                             <  SRR4302541.898064/94‑1 (MQ=60)
                                                                   AGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCA                             <  SRR4302541.8676993/94‑1 (MQ=60)
                                                                    GCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAT                            <  SRR4302541.4862232/94‑1 (MQ=60)
                                                                    GCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAT                            >  SRR4302541.5546317/1‑94 (MQ=60)
                                                                    GCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAT                            >  SRR4302541.5546059/1‑94 (MQ=60)
                                                                     CTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATC                           <  SRR4302541.5811516/94‑1 (MQ=60)
                                                                     CTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATC                           <  SRR4302541.7985547/94‑1 (MQ=60)
                                                                      TTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCA                          <  SRR4302541.1872439/94‑1 (MQ=60)
                                                                       TTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCAC                         <  SRR4302541.5169896/94‑1 (MQ=60)
                                                                        TGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACG                        >  SRR4302541.4193392/1‑94 (MQ=60)
                                                                         GCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGA                       >  SRR4302541.3861922/1‑94 (MQ=60)
                                                                          CTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAC                      >  SRR4302541.5235203/1‑94 (MQ=60)
                                                                           TTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACC                     <  SRR4302541.8813872/94‑1 (MQ=60)
                                                                           TTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACC                     >  SRR4302541.4838408/1‑94 (MQ=60)
                                                                           TTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACC                     <  SRR4302541.5289406/94‑1 (MQ=60)
                                                                            TTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCG                    >  SRR4302541.2848003/1‑94 (MQ=60)
                                                                            TTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCG                    <  SRR4302541.8473668/94‑1 (MQ=60)
                                                                            TTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCG                    >  SRR4302541.7112550/1‑94 (MQ=60)
                                                                             TCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCGCATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGA                   <  SRR4302541.6775141/94‑1 (MQ=60)
                                                                              CTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAG                  >  SRR4302541.8243748/1‑94 (MQ=60)
                                                                              CTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAG                  <  SRR4302541.8898872/94‑1 (MQ=60)
                                                                              CTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAG                  <  SRR4302541.8898873/94‑1 (MQ=60)
                                                                              CTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAG                  >  SRR4302541.7038220/1‑94 (MQ=60)
                                                                               TTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGG                 >  SRR4302541.6370080/1‑94 (MQ=60)
                                                                                 CAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA               >  SRR4302541.5795839/1‑94 (MQ=60)
                                                                                 CAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA               >  SRR4302541.302141/1‑94 (MQ=60)
                                                                                  AACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTGCAGGTAAACCATCACGACCGAGGAAG              >  SRR4302541.2244889/1‑94 (MQ=60)
                                                                                  AACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAG              >  SRR4302541.3139956/1‑94 (MQ=60)
                                                                                  AACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAG              >  SRR4302541.6556162/1‑94 (MQ=60)
                                                                                    CGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGAC            >  SRR4302541.363518/1‑94 (MQ=60)
                                                                                     GTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACT           <  SRR4302541.7085281/94‑1 (MQ=60)
                                                                                     GTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACT           >  SRR4302541.1011421/1‑94 (MQ=60)
                                                                                       ctTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTT         <  SRR4302541.8751668/92‑1 (MQ=60)
                                                                                       T‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTA        <  SRR4302541.8376196/94‑1 (MQ=60)
                                                                                          TTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAAT      <  SRR4302541.8292817/94‑1 (MQ=60)
                                                                                             TTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCAT   <  SRR4302541.1338065/94‑1 (MQ=60)
                                                                                             TTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCAT   <  SRR4302541.7844963/94‑1 (MQ=60)
                                                                                             TTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCAT   <  SRR4302541.7405033/94‑1 (MQ=60)
                                                                                             TTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCAT   <  SRR4302541.6036849/94‑1 (MQ=60)
                                                                                              TGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATG  <  SRR4302541.4415373/94‑1 (MQ=60)
                                                                                              TGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATG  >  SRR4302541.7555728/1‑94 (MQ=60)
                                                                                              |                                                                                             
CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATG  >  NC_000913/1415903‑1416089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑