Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,415,996	(T)_6→7	coding (37/171 nt)	ydaE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,415,990	1	.	T	99.1%	839.2 / ‑0.4	212	coding (43/171 nt)	ydaE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (115/95); total (116/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 246 total aligned reads displayed.

AGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAG  >  NC_000913/1415898‑1416077

aGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCTACGTc                                                                                            >  1:1862397/1‑93 (MQ=255)

aGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTc                                                                                            >  2:4669778/1‑93 (MQ=255)

 gCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑t                                                                                          <  1:6108709/93‑1 (MQ=255)

 gCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑t                                                                                          >  2:2122580/1‑93 (MQ=255)

  cTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tt                                                                                         >  1:6299755/1‑93 (MQ=255)

   ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttt                                                                                        >  2:3428693/1‑93 (MQ=255)

   ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttt                                                                                        >  2:489348/1‑93 (MQ=255)

     cACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttt                                                                                      <  1:3189608/93‑1 (MQ=255)

      aCTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttt                                                                                     >  2:2979286/1‑93 (MQ=255)

      aCTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttt                                                                                     >  1:3230284/1‑93 (MQ=255)

       cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttttt                                                                                    <  1:1647679/93‑2 (MQ=255)

       cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttttt                                                                                    <  1:3357834/93‑2 (MQ=255)

             tatTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAg                                                                             <  2:3212245/93‑1 (MQ=255)

              atTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAgg                                                                            <  2:1079500/93‑1 (MQ=255)

              atTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAgg                                                                            <  1:4221413/93‑1 (MQ=255)

               ttttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAggt                                                                           >  2:5649727/1‑93 (MQ=255)

                tttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAggtg                                                                          <  1:1178315/93‑1 (MQ=255)

                tttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAggtg                                                                          <  2:6032453/93‑1 (MQ=255)

                 ttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAggtgg                                                                         <  2:1677679/93‑1 (MQ=255)

                  tctcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAggtggt                                                                        >  2:2267041/1‑93 (MQ=255)

                     cGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAg                                                                     >  1:3466628/1‑93 (MQ=255)

                        ttCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGcac                                                                  >  1:6425596/1‑93 (MQ=255)

                         tCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGcaca                                                                 >  2:4107864/1‑93 (MQ=255)

                          cGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACat                                                                <  2:2802083/93‑1 (MQ=255)

                          cGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACat                                                                >  1:2757916/1‑93 (MQ=255)

                           gTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatt                                                               <  2:5821445/93‑1 (MQ=255)

                           gTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatt                                                               >  2:2347265/1‑93 (MQ=255)

                            tCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattt                                                              >  2:4229655/1‑93 (MQ=255)

                            tCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattt                                                              <  2:5381259/93‑1 (MQ=255)

                            tCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattt                                                              >  1:4067544/1‑93 (MQ=255)

                            tCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattt                                                              >  2:2759063/1‑93 (MQ=255)

                             cTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatttt                                                             >  1:6408983/1‑93 (MQ=255)

                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatttta                                                            <  2:4762180/93‑1 (MQ=255)

                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatttta                                                            <  1:5628217/93‑1 (MQ=255)

                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatttta                                                            >  1:566818/1‑93 (MQ=255)

                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatttta                                                            <  2:5164160/93‑1 (MQ=255)

                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACatttta                                                            >  1:5903297/1‑93 (MQ=255)

                               tttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattttat                                                           <  1:2891284/93‑1 (MQ=255)

                               tttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattttat                                                           <  1:4834788/93‑1 (MQ=255)

                                ttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattttatt                                                          <  2:549529/93‑1 (MQ=255)

                                 tGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattttattt                                                         <  1:3976992/93‑1 (MQ=255)

                                  gATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACattttatttt                                                        >  2:5063043/1‑93 (MQ=255)

                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTc                                                       <  2:1116994/93‑1 (MQ=255)

                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTc                                                       <  1:4747493/93‑1 (MQ=255)

                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTc                                                       <  2:802718/93‑1 (MQ=255)

                                     aaaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCtt                                                     >  2:6429006/1‑93 (MQ=255)

                                     aaaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCtt                                                     >  2:144369/1‑93 (MQ=255)

                                      aaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTa                                                    <  1:5539596/93‑1 (MQ=255)

                                        aTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGt                                                  >  2:3059667/1‑93 (MQ=255)

                                         tCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtc                                                 >  2:1518743/1‑93 (MQ=255)

                                         tCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtc                                                 <  2:6362759/93‑1 (MQ=255)

                                         tCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtc                                                 >  2:3911897/1‑93 (MQ=255)

                                         tCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtc                                                 >  1:2986121/1‑93 (MQ=255)

                                          cccGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtca                                                <  2:1712194/93‑1 (MQ=255)

                                          cccGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtca                                                <  2:5938593/93‑1 (MQ=255)

                                           ccGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcat                                               >  1:2309531/1‑93 (MQ=255)

                                            cGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcatc                                              <  1:3403957/93‑1 (MQ=255)

                                            cGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGtcatc                                              <  1:1021913/93‑1 (MQ=255)

                                               gCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCAcc                                           >  2:2510046/1‑93 (MQ=255)

                                                cATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                          <  2:1037986/93‑1 (MQ=255)

                                                 aTGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAt                                         <  1:2550507/93‑1 (MQ=255)

                                                 aTGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAt                                         <  1:16982/93‑1 (MQ=255)

                                                  tgaagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATg                                        >  2:5641926/1‑93 (MQ=255)

                                                  tgaagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATg                                        >  2:1873340/1‑93 (MQ=255)

                                                   gaagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGa                                       >  2:3631095/1‑93 (MQ=255)

                                                     agtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACt                                     >  2:4008877/1‑93 (MQ=255)

                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                    <  1:701229/93‑1 (MQ=255)

                                                       tgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTcc                                   >  1:386584/1‑93 (MQ=255)

                                                        gaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCg                                  >  2:5666525/1‑93 (MQ=255)

                                                        gaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCg                                  >  2:916023/1‑93 (MQ=255)

                                                         aagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGc                                 >  1:5534558/1‑93 (MQ=255)

                                                          agCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGcc                                <  2:2902143/93‑1 (MQ=255)

                                                          agCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGcc                                >  2:4447547/1‑93 (MQ=255)

                                                           gCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCt                               >  2:3714419/1‑93 (MQ=255)

                                                             aTTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCttt                             >  2:5159957/1‑93 (MQ=255)

                                                             aTTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCttt                             >  1:6006482/1‑93 (MQ=255)

                                                              ttCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTa                            >  2:3394305/1‑93 (MQ=255)

                                                                cTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACa                          >  1:5751606/1‑93 (MQ=255)

                                                                 tttCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAg                         >  2:500621/1‑93 (MQ=255)

                                                                   tCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGt                       >  2:6368879/1‑93 (MQ=255)

                                                                   tCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGt                       >  1:4548112/1‑93 (MQ=255)

                                                                   tCAATAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGt                       >  2:1194520/1‑93 (MQ=255)

                                                                      aTAGCTTTGCTTTCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaaa                    <  1:2859012/93‑1 (MQ=255)

                                                                      aTAGCTTTGCTTTCTTCAACGTC‑TTTTTTTGGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaa                     >  2:2728692/1‑93 (MQ=255)

                                                                         gctttgctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCa                 >  1:3676057/1‑93 (MQ=255)

                                                                         gctttgctttCTTCAACGTC‑TTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAt                <  2:1395659/93‑1 (MQ=255)

                                                                           tttgctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATc               <  2:3529971/93‑1 (MQ=255)

                                                                            ttgctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCa              >  1:948369/1‑93 (MQ=255)

                                                                              gctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACg            >  1:4966976/1‑93 (MQ=255)

                                                                              gctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACg            >  1:2624908/1‑93 (MQ=255)

                                                                               ctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGa           <  1:5295157/93‑1 (MQ=255)

                                                                               ctttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGa           <  1:6331640/93‑1 (MQ=255)

                                                                                tttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAc          <  2:660976/93‑1 (MQ=255)

                                                                                tttCTTCAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAc          <  1:119472/93‑1 (MQ=255)

                                                                                 ttcttcAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAcc         >  2:2483540/1‑93 (MQ=255)

                                                                                     tcAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg     <  1:5851840/93‑1 (MQ=255)

                                                                                     tcAACGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg     <  1:1518743/93‑1 (MQ=255)

                                                                                       aaCGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGaa   >  1:5429938/1‑93 (MQ=255)

                                                                                       aaCGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGaa   >  1:3520660/1‑93 (MQ=255)

                                                                                        aCGTCTTTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAg  >  2:6224675/1‑93 (MQ=255)

AGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTT‑‑GCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAG  >  NC_000913/1415898‑1416077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATG  >  NC_000913/1415903‑1416089

CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTT                                                                                               <  SRR4302534.3274039/94‑1 (MQ=60)

CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTT                                                                                               >  SRR4302534.3316128/1‑94 (MQ=60)

 ACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTT                                                                                              <  SRR4302534.3667562/94‑1 (MQ=60)

  CTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTtg                                                                                             <  SRR4302534.3447771/94‑3 (MQ=60)

  CTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTtg                                                                                             <  SRR4302534.1690624/94‑3 (MQ=60)

   TGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTtgc                                                                                            >  SRR4302534.6099845/1‑91 (MQ=60)

     CAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAA                                                                                           >  SRR4302534.2578827/1‑94 (MQ=60)

      AATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAA                                                                                          <  SRR4302534.3250739/94‑1 (MQ=60)

         ATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGT                                                                                       >  SRR4302534.6566629/1‑94 (MQ=60)

         ATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGT                                                                                       <  SRR4302534.4335214/94‑1 (MQ=60)

          TTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTG                                                                                      <  SRR4302534.6471436/94‑1 (MQ=60)

           TTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGG                                                                                     <  SRR4302534.1209288/94‑1 (MQ=60)

            TTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGT                                                                                    >  SRR4302534.2246150/1‑94 (MQ=60)

              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  <  SRR4302534.6540148/94‑1 (MQ=60)

              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  >  SRR4302534.6620746/1‑94 (MQ=60)

              CTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAA                                                                                  <  SRR4302534.4948295/94‑1 (MQ=60)

               TCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAG                                                                                 <  SRR4302534.1020082/94‑1 (MQ=60)

               TCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAG                                                                                 >  SRR4302534.3559811/1‑94 (MQ=60)

                  GTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCAC                                                                              >  SRR4302534.6598890/1‑94 (MQ=60)

                   TTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACA                                                                             >  SRR4302534.1725852/1‑94 (MQ=60)

                    TCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACAT                                                                            >  SRR4302534.2830563/1‑94 (MQ=60)

                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302534.5981880/1‑94 (MQ=60)

                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302534.2654837/1‑94 (MQ=60)

                      GTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTT                                                                          >  SRR4302534.4176676/1‑94 (MQ=60)

                       TCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTT                                                                         >  SRR4302534.6581944/1‑94 (MQ=60)

                       TCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTT                                                                         <  SRR4302534.6463228/94‑1 (MQ=60)

                        CTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTA                                                                        >  SRR4302534.580737/1‑94 (MQ=60)

                        CTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTA                                                                        <  SRR4302534.3291683/94‑1 (MQ=60)

                        CTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTA                                                                        >  SRR4302534.3742281/1‑94 (MQ=60)

                        CTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTA                                                                        >  SRR4302534.6063581/1‑94 (MQ=60)

                         TTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTAT                                                                       <  SRR4302534.1592197/94‑1 (MQ=60)

                         TTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTAT                                                                       <  SRR4302534.5780536/94‑1 (MQ=60)

                          TTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATT                                                                      <  SRR4302534.2967152/94‑1 (MQ=60)

                          TTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATT                                                                      <  SRR4302534.353321/94‑1 (MQ=60)

                          TTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATT                                                                      <  SRR4302534.4965963/94‑1 (MQ=60)

                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    <  SRR4302534.401388/94‑1 (MQ=60)

                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    <  SRR4302534.4206986/94‑1 (MQ=60)

                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    <  SRR4302534.5126877/94‑1 (MQ=60)

                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    <  SRR4302534.4083415/94‑1 (MQ=60)

                            TGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTT                                                                    >  SRR4302534.2137103/1‑94 (MQ=60)

                              ATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCT                                                                  <  SRR4302534.4876364/94‑1 (MQ=60)

                                 AAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAG                                                               <  SRR4302534.5689127/94‑1 (MQ=60)

                                 AAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAG                                                               <  SRR4302534.3158337/94‑1 (MQ=60)

                                 AAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAG                                                               <  SRR4302534.1287231/94‑1 (MQ=60)

                                 AAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAG                                                               <  SRR4302534.60220/94‑1 (MQ=60)

                                   ATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTC                                                             <  SRR4302534.4466124/94‑1 (MQ=60)

                                   ATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTC                                                             >  SRR4302534.3064220/1‑94 (MQ=60)

                                     CCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCAT                                                           >  SRR4302534.2618429/1‑94 (MQ=60)

                                     CCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCAT                                                           >  SRR4302534.2370830/1‑94 (MQ=60)

                                       CGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCA                                                         <  SRR4302534.3495363/94‑1 (MQ=60)

                                       CGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCA                                                         <  SRR4302534.6271051/94‑1 (MQ=60)

                                       CGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCA                                                         <  SRR4302534.1048227/94‑1 (MQ=60)

                                       CGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCA                                                         <  SRR4302534.304661/94‑1 (MQ=60)

                                           CATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAT                                                     >  SRR4302534.4732604/1‑94 (MQ=60)

                                           CATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCAT                                                     <  SRR4302534.3451869/94‑1 (MQ=60)

                                            ATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATG                                                    <  SRR4302534.122635/94‑1 (MQ=60)

                                            ATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATG                                                    <  SRR4302534.2618125/94‑1 (MQ=60)

                                            ATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATG                                                    <  SRR4302534.17433/94‑1 (MQ=60)

                                             TGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGA                                                   >  SRR4302534.1743587/1‑94 (MQ=60)

                                              GAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGAC                                                  >  SRR4302534.60410/1‑94 (MQ=60)

                                               AAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACT                                                 <  SRR4302534.747497/94‑1 (MQ=60)

                                                 GTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCC                                               >  SRR4302534.396475/1‑94 (MQ=60)

                                                 GTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCC                                               <  SRR4302534.718415/94‑1 (MQ=60)

                                                  TGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCG                                              >  SRR4302534.6613748/1‑94 (MQ=60)

                                                   GAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGC                                             >  SRR4302534.5683953/1‑94 (MQ=60)

                                                   GAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGC                                             >  SRR4302534.3328419/1‑94 (MQ=60)

                                                    AAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCC                                            >  SRR4302534.3547270/1‑94 (MQ=60)

                                                     AGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCT                                           >  SRR4302534.4293686/1‑94 (MQ=60)

                                                     AGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCT                                           <  SRR4302534.3781230/94‑1 (MQ=60)

                                                       CATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTT                                         >  SRR4302534.6169666/1‑94 (MQ=60)

                                                        ATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTA                                        >  SRR4302534.557626/1‑94 (MQ=60)

                                                         TTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTAC                                       <  SRR4302534.5695356/94‑1 (MQ=60)

                                                          TCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACA                                      >  SRR4302534.5907570/1‑94 (MQ=60)

                                                           CTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAG                                     <  SRR4302534.3427463/94‑1 (MQ=60)

                                                             TTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGT                                   >  SRR4302534.4671412/1‑94 (MQ=60)

                                                               CAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAA                                 >  SRR4302534.5001697/1‑94 (MQ=60)

                                                                AATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAA                                >  SRR4302534.5727313/1‑94 (MQ=60)

                                                                 ATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAAC                               <  SRR4302534.2934106/94‑1 (MQ=60)

                                                                  TAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGAGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACC                              >  SRR4302534.1297952/1‑94 (MQ=60)

                                                                   AGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCA                             >  SRR4302534.3774873/1‑94 (MQ=60)

                                                                   AGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCA                             >  SRR4302534.1001099/1‑94 (MQ=60)

                                                                     CTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATC                           <  SRR4302534.5484725/94‑1 (MQ=60)

                                                                      TTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCGGCCTTTACAGGTAAACCATCA                          <  SRR4302534.5393296/94‑1 (MQ=60)

                                                                      TTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCA                          >  SRR4302534.972506/1‑94 (MQ=60)

                                                                       TTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCAC                         >  SRR4302534.2956911/1‑94 (MQ=60)

                                                                        TGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACG                        >  SRR4302534.2694345/1‑94 (MQ=60)

                                                                        TGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACG                        >  SRR4302534.5101456/1‑94 (MQ=60)

                                                                         GCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGA                       >  SRR4302534.3167883/1‑94 (MQ=60)

                                                                         GCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGA                       >  SRR4302534.4965096/1‑94 (MQ=60)

                                                                         GCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGA                       >  SRR4302534.2580057/1‑94 (MQ=60)

                                                                          CTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAC                      >  SRR4302534.5632468/1‑94 (MQ=60)

                                                                          CTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAC                      <  SRR4302534.6502702/94‑1 (MQ=60)

                                                                          CTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAC                      <  SRR4302534.5438072/94‑1 (MQ=60)

                                                                          CTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGAATCCGCCTTTACAGGTAAACCATCACGAC                      <  SRR4302534.2886239/94‑1 (MQ=60)

                                                                           TTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACC                     <  SRR4302534.3568411/94‑1 (MQ=60)

                                                                           TTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACC                     <  SRR4302534.122644/94‑1 (MQ=60)

                                                                            TTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCG                    >  SRR4302534.3973672/1‑94 (MQ=60)

                                                                              CTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAG                  >  SRR4302534.6457688/1‑94 (MQ=60)

                                                                               TTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGG                 >  SRR4302534.4092516/1‑94 (MQ=60)

                                                                                TCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGA                <  SRR4302534.6010738/94‑1 (MQ=60)

                                                                                TCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGA                <  SRR4302534.1137200/94‑1 (MQ=60)

                                                                                TCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGA                <  SRR4302534.1558631/94‑1 (MQ=60)

                                                                                 CAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA               >  SRR4302534.5576099/1‑94 (MQ=60)

                                                                                 CAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA               >  SRR4302534.2852432/1‑94 (MQ=60)

                                                                                 CAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAA               >  SRR4302534.3615360/1‑94 (MQ=60)

                                                                                     GTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACT           >  SRR4302534.6258289/1‑94 (MQ=60)

                                                                                     GTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACT           >  SRR4302534.6244426/1‑94 (MQ=60)

                                                                                     GTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACT           >  SRR4302534.311928/1‑94 (MQ=60)

                                                                                      tctTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTT          >  SRR4302534.732346/4‑94 (MQ=60)

                                                                                      tctTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTT          >  SRR4302534.5640135/4‑94 (MQ=60)

                                                                                      tctTTTTTTGCAAAGGCGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGAATT          >  SRR4302534.5447515/4‑94 (MQ=60)

                                                                                       ctTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTT         <  SRR4302534.5597434/92‑1 (MQ=60)

                                                                                       ctTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTT         <  SRR4302534.3857646/92‑1 (MQ=60)

                                                                                       ctTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTT         >  SRR4302534.1873552/3‑94 (MQ=60)

                                                                                           TTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATC     >  SRR4302534.679953/1‑94 (MQ=60)

                                                                                              TGCAAAGGTGGTATGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGCAAACCATCACGACCGAGGAAGAaattttttttt  >  SRR4302534.5028425/1‑83 (MQ=60)

CACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATG  >  NC_000913/1415903‑1416089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`