Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,636,465	T→C	intergenic (‑296/+14)	b1551 ← / ← cspI	hypothetical protein/cold shock‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,636,465	0	T	C	99.6%	969.1 / ‑4.8	276	intergenic (‑296/+14)	b1551/cspI	hypothetical protein/cold shock‑like protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (137/138); total (137/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

Only 100 of 281 total aligned reads displayed.

CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTC  >  NC_000913/1636375‑1636553

 cccGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAat                                                                                       >  1:1707328/1‑93 (MQ=255)

 cccGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAat                                                                                       >  1:814001/1‑93 (MQ=255)

   cGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAatat                                                                                     >  2:3092857/1‑93 (MQ=255)

   cGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAatat                                                                                     <  2:1313420/93‑1 (MQ=255)

    gAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATAtt                                                                                    <  2:2629833/93‑1 (MQ=255)

    gAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATAtt                                                                                    >  2:6212246/1‑93 (MQ=255)

    gAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATAtt                                                                                    <  1:8213370/93‑1 (MQ=255)

    gAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATAtt                                                                                    <  2:4568235/93‑1 (MQ=255)

      aaGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGt                                                                                  <  1:6486607/93‑1 (MQ=255)

       aGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTc                                                                                 <  2:3630374/93‑1 (MQ=255)

       aGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTc                                                                                 <  1:987605/93‑1 (MQ=255)

        ggATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCt                                                                                <  2:6019055/93‑1 (MQ=255)

         gATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTa                                                                               >  1:523318/1‑93 (MQ=255)

          aTTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTAc                                                                              <  2:4853226/93‑1 (MQ=255)

           ttCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTAcc                                                                             <  1:845144/93‑1 (MQ=255)

           ttCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTAcc                                                                             >  1:5040222/1‑93 (MQ=255)

              aTGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCa                                                                          >  2:6871515/1‑93 (MQ=255)

              aTGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCa                                                                          >  1:3691425/1‑93 (MQ=255)

                gTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCaaa                                                                        <  2:7095858/93‑1 (MQ=255)

                gTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCaaa                                                                        <  1:8783115/93‑1 (MQ=255)

                gTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCaaa                                                                        <  1:6574514/93‑1 (MQ=255)

                 tCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACCGGCATCTAAAGTGAATATGGTTTGCAATCTTGTCTACCTCAAAg                                                                       >  1:5199593/1‑93 (MQ=255)

                   aGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAgcg                                                                     >  1:5512040/1‑93 (MQ=255)

                   aGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAgcg                                                                     >  2:4414995/1‑93 (MQ=255)

                    gAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAgcgc                                                                    >  1:3458330/1‑93 (MQ=255)

                    gAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAgcgc                                                                    >  1:3835180/1‑93 (MQ=255)

                       aCATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCAc                                                                 >  1:2884311/1‑93 (MQ=255)

                       aCATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCAc                                                                 >  2:7507835/1‑93 (MQ=255)

                         aTCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTa                                                               <  2:5708353/93‑1 (MQ=255)

                         aTCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTa                                                               <  1:1451273/93‑1 (MQ=255)

                           cAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACa                                                             <  2:8168566/93‑1 (MQ=255)

                            aaaaGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACAt                                                            <  1:1005058/93‑1 (MQ=255)

                            aaaaGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACAt                                                            <  1:2298683/93‑1 (MQ=255)

                            aaaaGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACAt                                                            >  1:5282385/1‑93 (MQ=255)

                            aaaaGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACAt                                                            <  2:5185650/93‑1 (MQ=255)

                             aaaGATAATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATg                                                           >  1:4078364/1‑93 (MQ=255)

                              aaGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGa                                                          <  1:7894031/93‑1 (MQ=255)

                                gatgatGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAAc                                                        >  2:5079801/1‑93 (MQ=255)

                                 atgatgTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACa                                                       <  2:4398707/93‑1 (MQ=255)

                                  tgatgTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAg                                                      >  1:75276/1‑93 (MQ=255)

                                   gatgTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGc                                                     <  1:4634968/93‑1 (MQ=255)

                                   gatgTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGc                                                     <  2:467830/93‑1 (MQ=255)

                                      gTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGc                                                  >  1:2427952/1‑93 (MQ=255)

                                       ttCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCa                                                 <  2:6687258/93‑1 (MQ=255)

                                        tCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAg                                                >  2:1539745/1‑93 (MQ=255)

                                        tCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAg                                                <  1:6016062/93‑1 (MQ=255)

                                        tCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAg                                                >  2:4483232/1‑93 (MQ=255)

                                        tCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAg                                                <  2:5432972/93‑1 (MQ=255)

                                         cTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAgg                                               <  2:2194323/93‑1 (MQ=255)

                                           ataCTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGAc                                             >  2:1156040/1‑93 (MQ=255)

                                            taCTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGAcc                                            <  1:7144097/93‑1 (MQ=255)

                                            taCTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGAcc                                            <  1:1120453/93‑1 (MQ=255)

                                             aCTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCt                                           <  1:6752316/93‑1 (MQ=255)

                                              cTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCtt                                          >  1:5159799/1‑93 (MQ=255)

                                               tGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCttt                                         <  2:5756557/93‑1 (MQ=255)

                                                gggAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTa                                        <  2:7156657/93‑1 (MQ=255)

                                                aggAACCAATGCAACACGGGCACCTAAAGTGAATATGGTTTTCAATATTTTCAACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTa                                        <  2:6346531/92‑1 (MQ=255)

                                                  gAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAgg                                      >  2:7333880/1‑93 (MQ=255)

                                                  gAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAgg                                      >  2:7621523/1‑93 (MQ=255)

                                                    aCCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTc                                    <  2:3359152/93‑1 (MQ=255)

                                                      cATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCg                                  >  2:3966388/1‑93 (MQ=255)

                                                       aTGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGt                                 <  1:959065/93‑1 (MQ=255)

                                                       aTGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGt                                 >  1:6318348/1‑93 (MQ=255)

                                                       aTGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGt                                 <  1:8070282/93‑1 (MQ=255)

                                                         gACAACACGGGCATCTAAAGTGAATATTGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTtc                               <  1:893288/93‑1 (MQ=255)

                                                          acaacaCGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTtct                              <  2:2327402/93‑1 (MQ=255)

                                                             acgcGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGGCCTTTAGGTCCGTTCTCaa                           <  2:2234661/90‑1 (MQ=255)

                                                             acacGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCaa                           <  1:3122783/93‑1 (MQ=255)

                                                              cacGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAAt                          <  2:7919906/93‑1 (MQ=255)

                                                              cacGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAAt                          <  1:662691/93‑1 (MQ=255)

                                                               acGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATa                         >  1:6141059/1‑93 (MQ=255)

                                                                cGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATAc                        <  1:2811504/93‑1 (MQ=255)

                                                                  ggCATCTAAAGTGAATATGGTTTGCAATATTTGCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTACGTCCGTTCTCAATACCa                      >  1:8350170/1‑93 (MQ=255)

                                                                  ggCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCa                      <  1:3576754/93‑1 (MQ=255)

                                                                  ggCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCa                      <  1:455658/93‑1 (MQ=255)

                                                                    cATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCaaa                    <  1:5943069/93‑1 (MQ=255)

                                                                       cTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTc                 <  1:8749920/93‑1 (MQ=255)

                                                                        tAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCa                <  2:8113106/93‑1 (MQ=255)

                                                                        tAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCa                >  1:1978522/1‑93 (MQ=255)

                                                                         aaaGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCaa               >  2:3691671/1‑93 (MQ=255)

                                                                          aaGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAAc              <  2:847499/93‑1 (MQ=255)

                                                                          aaGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAAc              <  2:8608641/93‑1 (MQ=255)

                                                                          aaGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAAc              >  2:417591/1‑93 (MQ=255)

                                                                            gTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACtt            <  1:3491509/93‑1 (MQ=255)

                                                                              gAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTcc          >  2:6591059/1‑93 (MQ=255)

                                                                              gAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTcc          <  1:4915583/93‑1 (MQ=255)

                                                                              gAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTcc          <  2:7988277/93‑1 (MQ=255)

                                                                               aaTATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCt         >  2:5340936/1‑93 (MQ=255)

                                                                               aaTATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCt         >  2:7888614/1‑93 (MQ=255)

                                                                                atatGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTg        >  1:580190/1‑93 (MQ=255)

                                                                                atatGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTg        >  1:2907595/1‑93 (MQ=255)

                                                                                 tatGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTgt       >  1:2159524/1‑92 (MQ=255)

                                                                                 tatGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGa       >  2:2884586/1‑93 (MQ=255)

                                                                                 tatGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGa       >  1:1312721/1‑93 (MQ=255)

                                                                                 tatGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGa       <  2:1782232/93‑1 (MQ=255)

                                                                                  atGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGAt      <  1:735884/93‑1 (MQ=255)

                                                                                    ggTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATtc    >  1:6258143/1‑93 (MQ=255)

                                                                                      tttGCAATTTTTTCTACCTCAAAGCCCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATtctc  <  2:7865574/93‑1 (MQ=255)

                                                                                      tttGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATtctc  >  2:8252826/1‑93 (MQ=255)

                                                                                      tttGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATtctc  <  2:1890150/93‑1 (MQ=255)

CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTC  >  NC_000913/1636375‑1636553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTA  >  NC_000913/1636373‑1636558

TGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCA                                                                                              >  SRR4302541.4533920/1‑94 (MQ=60)

 GCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAA                                                                                             >  SRR4302541.8300211/1‑94 (MQ=60)

  CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAAT                                                                                            <  SRR4302541.4810104/94‑1 (MQ=60)

  CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAAT                                                                                            <  SRR4302541.5977038/94‑1 (MQ=60)

  CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAAT                                                                                            >  SRR4302541.2588014/1‑94 (MQ=60)

  CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAAT                                                                                            >  SRR4302541.1724038/1‑94 (MQ=60)

  CCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAAT                                                                                            >  SRR4302541.821434/1‑94 (MQ=60)

   CCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATA                                                                                           <  SRR4302541.5321188/94‑1 (MQ=60)

   CCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATA                                                                                           >  SRR4302541.7275849/1‑94 (MQ=60)

     CGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATT                                                                                         >  SRR4302541.4622926/1‑94 (MQ=60)

     CGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATT                                                                                         >  SRR4302541.8467055/1‑94 (MQ=60)

      GAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTG                                                                                        <  SRR4302541.99305/94‑1 (MQ=60)

      GAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTG                                                                                        <  SRR4302541.3516905/94‑1 (MQ=60)

      GAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTG                                                                                        <  SRR4302541.8302973/94‑1 (MQ=60)

      GAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTG                                                                                        <  SRR4302541.8302972/94‑1 (MQ=60)

        AAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTC                                                                                      <  SRR4302541.6552725/94‑1 (MQ=60)

        AAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTC                                                                                      <  SRR4302541.1868604/94‑1 (MQ=60)

         AGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCT                                                                                     <  SRR4302541.996814/94‑1 (MQ=60)

          GGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTA                                                                                    >  SRR4302541.528211/1‑94 (MQ=60)

          GGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTA                                                                                    <  SRR4302541.5264682/94‑1 (MQ=60)

           GATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTAC                                                                                   <  SRR4302541.4519774/94‑1 (MQ=60)

           GATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTAC                                                                                   <  SRR4302541.7232262/94‑1 (MQ=60)

            ATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACC                                                                                  <  SRR4302541.6677683/94‑1 (MQ=60)

            ATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACC                                                                                  >  SRR4302541.5092084/1‑94 (MQ=60)

            ATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACC                                                                                  >  SRR4302541.5092083/1‑94 (MQ=60)

             TTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCT                                                                                 <  SRR4302541.5552664/94‑1 (MQ=60)

             TTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCT                                                                                 <  SRR4302541.852821/94‑1 (MQ=60)

               CATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCA                                                                               >  SRR4302541.3728772/1‑94 (MQ=60)

               CATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCA                                                                               >  SRR4302541.6590832/1‑94 (MQ=60)

               CATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCA                                                                               >  SRR4302541.127687/1‑94 (MQ=60)

                ATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAA                                                                              >  SRR4302541.1890372/1‑94 (MQ=60)

                  GTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAG                                                                            <  SRR4302541.8876967/94‑1 (MQ=60)

                  GTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAG                                                                            >  SRR4302541.5253137/1‑94 (MQ=60)

                  GTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAG                                                                            <  SRR4302541.8876968/94‑1 (MQ=60)

                  GTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAG                                                                            <  SRR4302541.6641776/94‑1 (MQ=60)

                  GTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACCGGCATCTAAAGTGAATATGGTTTGCAATCTTGTCTACCTCAAAG                                                                            >  SRR4302541.5253136/1‑94 (MQ=60)

                    CAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCG                                                                          >  SRR4302541.5568480/1‑94 (MQ=60)

                    CAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCG                                                                          <  SRR4302541.629325/94‑1 (MQ=60)

                    CAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCG                                                                          >  SRR4302541.3758650/1‑94 (MQ=60)

                     AGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGC                                                                         >  SRR4302541.3492885/1‑94 (MQ=60)

                     AGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGC                                                                         >  SRR4302541.3492886/1‑94 (MQ=60)

                     AGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGC                                                                         >  SRR4302541.7500635/1‑94 (MQ=60)

                     AGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGC                                                                         >  SRR4302541.4013749/1‑94 (MQ=60)

                     AGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGC                                                                         >  SRR4302541.6835207/1‑94 (MQ=60)

                     AGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGC                                                                         >  SRR4302541.3873814/1‑94 (MQ=60)

                      GAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCC                                                                        >  SRR4302541.814812/1‑94 (MQ=60)

                      GAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCC                                                                        <  SRR4302541.7600606/94‑1 (MQ=60)

                        AACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCAC                                                                      >  SRR4302541.2913266/1‑94 (MQ=60)

                         ACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACT                                                                     <  SRR4302541.6011275/94‑1 (MQ=60)

                          CATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTA                                                                    >  SRR4302541.3585406/1‑94 (MQ=60)

                           ATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTAC                                                                   <  SRR4302541.1465419/94‑1 (MQ=60)

                            TCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACA                                                                  >  SRR4302541.8510384/1‑94 (MQ=60)

                             CAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACAT                                                                 <  SRR4302541.3841329/94‑1 (MQ=60)

                             CAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACAT                                                                 >  SRR4302541.5336772/1‑94 (MQ=60)

                              AAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATG                                                                <  SRR4302541.2320971/94‑1 (MQ=60)

                              AAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATG                                                                <  SRR4302541.1014391/94‑1 (MQ=60)

                              AAAAGATAATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATG                                                                >  SRR4302541.4118618/1‑94 (MQ=60)

                               AAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGA                                                               >  SRR4302541.1018683/1‑94 (MQ=60)

                               AAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGA                                                               >  SRR4302541.1721035/1‑94 (MQ=60)

                                AAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAA                                                              <  SRR4302541.7980830/94‑1 (MQ=60)

                                AAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAA                                                              <  SRR4302541.2222357/94‑1 (MQ=60)

                                AAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAA                                                              <  SRR4302541.6378280/94‑1 (MQ=60)

                                AAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAA                                                              >  SRR4302541.291860/1‑94 (MQ=60)

                                   ATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAG                                                           >  SRR4302541.75915/1‑94 (MQ=60)

                                   ATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAG                                                           >  SRR4302541.5188163/1‑94 (MQ=60)

                                   ATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAG                                                           <  SRR4302541.5791189/94‑1 (MQ=60)

                                    TGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGC                                                          >  SRR4302541.3373550/1‑94 (MQ=60)

                                     GATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCG                                                         <  SRR4302541.4681849/94‑1 (MQ=60)

                                     GATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCG                                                         <  SRR4302541.4682136/94‑1 (MQ=60)

                                      ATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGG                                                        >  SRR4302541.2504740/1‑94 (MQ=60)

                                      ATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGG                                                        >  SRR4302541.888484/1‑94 (MQ=60)

                                      ATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGG                                                        >  SRR4302541.8082131/1‑94 (MQ=60)

                                      ATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGCGAATATGCTTTGCAATATGGTCTACCTCAAAGCGCCACTAGATCAACAGCGG                                                        >  SRR4302541.888485/1‑94 (MQ=60)

                                       TGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGC                                                       >  SRR4302541.2451954/1‑94 (MQ=60)

                                       TGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGC                                                       >  SRR4302541.164778/1‑94 (MQ=60)

                                          TCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGG                                                    >  SRR4302541.5230341/1‑94 (MQ=60)

                                          TCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGG                                                    <  SRR4302541.6077718/94‑1 (MQ=60)

                                           CTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGA                                                   <  SRR4302541.2901533/94‑1 (MQ=60)

                                           CTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGA                                                   <  SRR4302541.608807/94‑1 (MQ=60)

                                              TACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCT                                                <  SRR4302541.1130997/94‑1 (MQ=60)

                                              TACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCT                                                <  SRR4302541.7220231/94‑1 (MQ=60)

                                               ACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTT                                               <  SRR4302541.6824505/94‑1 (MQ=60)

                                               ACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTT                                               <  SRR4302541.6479146/94‑1 (MQ=60)

                                               ACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTT                                               >  SRR4302541.5212881/1‑94 (MQ=60)

                                                CTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTT                                              >  SRR4302541.8715580/1‑94 (MQ=60)

                                                 TGTGACCCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTA                                             <  SRR4302541.8319446/94‑1 (MQ=60)

                                                 TGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTA                                             <  SRR4302541.8751807/94‑1 (MQ=60)

                                                 TGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTA                                             >  SRR4302541.3434028/1‑94 (MQ=60)

                                                  GGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAG                                            <  SRR4302541.5304947/94‑1 (MQ=60)

                                                  GGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAG                                            >  SRR4302541.6494982/1‑94 (MQ=60)

                                                   GGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGG                                           <  SRR4302541.3657662/94‑1 (MQ=60)

                                                   GGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGG                                           <  SRR4302541.2144005/94‑1 (MQ=60)

                                                     AACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTC                                         >  SRR4302541.3993152/1‑94 (MQ=60)

                                                      ACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAccggaacgcacgttaggacc                                        >  SRR4302541.1514340/1‑74 (MQ=60)

                                                      ACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCC                                        <  SRR4302541.6787378/94‑1 (MQ=60)

                                                      ACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCC                                        <  SRR4302541.7898994/94‑1 (MQ=60)

                                                      ACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCA                                        <  SRR4302541.4827323/94‑1 (MQ=60)

                                                       CCATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCG                                       <  SRR4302541.1395248/94‑1 (MQ=60)

                                                        CATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGT                                      >  SRR4302541.6382944/1‑94 (MQ=60)

                                                        CATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGT                                      <  SRR4302541.948396/94‑1 (MQ=60)

                                                        CATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGT                                      >  SRR4302541.5774402/1‑94 (MQ=60)

                                                         ATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTT                                     <  SRR4302541.8158885/94‑1 (MQ=60)

                                                         ATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTT                                     >  SRR4302541.5145302/1‑94 (MQ=60)

                                                         ATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTT                                     <  SRR4302541.968112/94‑1 (MQ=60)

                                                         ATGACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTT                                     >  SRR4302541.6951042/1‑94 (MQ=60)

                                                           GACAACACGGGCATCTAAAGTGAATATTGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCT                                   <  SRR4302541.901508/94‑1 (MQ=60)

                                                           GACAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCT                                   >  SRR4302541.3598305/1‑94 (MQ=60)

                                                             CAACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCA                                 >  SRR4302541.5245601/1‑94 (MQ=60)

                                                              AACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAA                                >  SRR4302541.6563888/1‑94 (MQ=60)

                                                               ACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAAT                               <  SRR4302541.3154369/94‑1 (MQ=60)

                                                               ACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAAT                               <  SRR4302541.6069060/94‑1 (MQ=60)

                                                               ACACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAAT                               <  SRR4302541.7633716/94‑1 (MQ=60)

                                                                CACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATA                              <  SRR4302541.668938/94‑1 (MQ=60)

                                                                CACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATA                              <  SRR4302541.1261641/94‑1 (MQ=60)

                                                                CACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATA                              >  SRR4302541.2242837/1‑94 (MQ=60)

                                                                CACGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATA                              >  SRR4302541.6203780/1‑94 (MQ=60)

                                                                  CGGGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACC                            <  SRR4302541.2839914/94‑1 (MQ=60)

                                                                   GGGCATCTAAAGTGAATATGGTTTGCAATATTTGCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTACGTCCGTTCTCAATACCA                           >  SRR4302541.8440797/1‑94 (MQ=60)

                                                                    GGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAA                          <  SRR4302541.3612996/94‑1 (MQ=60)

                                                                    GGCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAA                          <  SRR4302541.459998/94‑1 (MQ=60)

                                                                     GCATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAA                         >  SRR4302541.840674/1‑94 (MQ=60)

                                                                      CATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAAT                        <  SRR4302541.6003620/94‑1 (MQ=60)

                                                                      CATCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAAT                        >  SRR4302541.61182/1‑94 (MQ=60)

                                                                        TCTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTC                      >  SRR4302541.1392832/1‑94 (MQ=60)

                                                                         CTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCA                     >  SRR4302541.1997636/1‑94 (MQ=60)

                                                                         CTAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCA                     <  SRR4302541.8843524/94‑1 (MQ=60)

                                                                          TAAAGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAA                    <  SRR4302541.2993972/94‑1 (MQ=60)

                                                                             AGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTT                 >  SRR4302541.8009132/1‑94 (MQ=60)

                                                                             AGTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTT                 >  SRR4302541.973949/1‑94 (MQ=60)

                                                                              GTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTC                <  SRR4302541.3526496/94‑1 (MQ=60)

                                                                              GTGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTC                >  SRR4302541.839476/1‑94 (MQ=60)

                                                                               TGAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCC               <  SRR4302541.5954662/94‑1 (MQ=60)

                                                                                GAATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCT              <  SRR4302541.4965749/94‑1 (MQ=60)

                                                                                 AATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTG             >  SRR4302541.2936828/1‑94 (MQ=60)

                                                                                 AATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTG             <  SRR4302541.906462/94‑1 (MQ=60)

                                                                                 AATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTG             >  SRR4302541.585655/1‑94 (MQ=60)

                                                                                 AATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTG             <  SRR4302541.4405889/94‑1 (MQ=60)

                                                                                  ATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGT            >  SRR4302541.2180411/1‑94 (MQ=60)

                                                                                  ATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGC            >  SRR4302541.308492/1‑94 (MQ=60)

                                                                                  ATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGA            >  SRR4302541.2450698/1‑94 (MQ=60)

                                                                                  ATATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGA            >  SRR4302541.1325642/1‑94 (MQ=60)

                                                                                    ATGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATT          <  SRR4302541.742889/94‑1 (MQ=60)

                                                                                     TGGTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTC         >  SRR4302541.6322066/1‑94 (MQ=60)

                                                                                       GTTTGCAATATTTTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTC       <  SRR4302541.6351009/94‑1 (MQ=60)

                                                                                       GTTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTC       <  SRR4302541.2361097/94‑1 (MQ=60)

                                                                                        TTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCA      <  SRR4302541.2891177/94‑1 (MQ=60)

                                                                                        TTTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCA      <  SRR4302541.6665966/94‑1 (MQ=60)

                                                                                         TTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAT     >  SRR4302541.4011171/1‑94 (MQ=60)

                                                                                         TTGCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAG     >  SRR4302541.1849963/1‑94 (MQ=60)

                                                                                           GCAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTT   <  SRR4302541.8238365/94‑1 (MQ=60)

                                                                                            CAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTA  <  SRR4302541.8435921/94‑1 (MQ=60)

TGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTA  >  NC_000913/1636373‑1636558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`